Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60722.t1 | XP_013469604.1 | 99.7 | 389 | 1 | 0 | 1 | 389 | 1 | 389 | 1.20E-220 | 775.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60722.t1 | P30076 | 99.0 | 389 | 4 | 0 | 1 | 389 | 1 | 389 | 2.8e-221 | 768.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60722.t1 | A0A072VPA5 | 99.7 | 389 | 1 | 0 | 1 | 389 | 1 | 389 | 9.0e-221 | 775.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene60722 | -0.800439 | 1.44E-48 | -1.69E-46 |
| MS.gene049181 | MS.gene60722 | 0.802357 | 5.83E-49 | -1.69E-46 |
| MS.gene049259 | MS.gene60722 | 0.812755 | 3.61E-51 | -1.69E-46 |
| MS.gene049281 | MS.gene60722 | 0.809644 | 1.71E-50 | -1.69E-46 |
| MS.gene049312 | MS.gene60722 | 0.803448 | 3.47E-49 | -1.69E-46 |
| MS.gene049320 | MS.gene60722 | -0.815915 | 7.24E-52 | -1.69E-46 |
| MS.gene049611 | MS.gene60722 | 0.805854 | 1.09E-49 | -1.69E-46 |
| MS.gene05015 | MS.gene60722 | 0.809357 | 1.97E-50 | -1.69E-46 |
| MS.gene050216 | MS.gene60722 | -0.807717 | 4.41E-50 | -1.69E-46 |
| MS.gene050363 | MS.gene60722 | 0.83058 | 2.71E-55 | -1.69E-46 |
| MS.gene050381 | MS.gene60722 | 0.879526 | 1.16E-69 | -1.69E-46 |
| MS.gene050504 | MS.gene60722 | -0.825885 | 3.67E-54 | -1.69E-46 |
| MS.gene050612 | MS.gene60722 | -0.82297 | 1.78E-53 | -1.69E-46 |
| MS.gene050868 | MS.gene60722 | -0.823178 | 1.59E-53 | -1.69E-46 |
| MS.gene050871 | MS.gene60722 | -0.822407 | 2.41E-53 | -1.69E-46 |
| MS.gene050883 | MS.gene60722 | 0.939193 | 1.92E-99 | -1.69E-46 |
| MS.gene050986 | MS.gene60722 | -0.802696 | 4.96E-49 | -1.69E-46 |
| MS.gene051044 | MS.gene60722 | 0.856706 | 2.68E-62 | -1.69E-46 |
| MS.gene05104 | MS.gene60722 | 0.800824 | 1.20E-48 | -1.69E-46 |
| MS.gene051127 | MS.gene60722 | 0.865213 | 6.95E-65 | -1.69E-46 |
| MS.gene051138 | MS.gene60722 | 0.830589 | 2.70E-55 | -1.69E-46 |
| MS.gene051364 | MS.gene60722 | 0.821356 | 4.22E-53 | -1.69E-46 |
| MS.gene051366 | MS.gene60722 | 0.80288 | 4.55E-49 | -1.69E-46 |
| MS.gene051410 | MS.gene60722 | 0.803942 | 2.74E-49 | -1.69E-46 |
| MS.gene05141 | MS.gene60722 | 0.827262 | 1.72E-54 | -1.69E-46 |
| MS.gene051946 | MS.gene60722 | 0.872768 | 2.47E-67 | -1.69E-46 |
| MS.gene052053 | MS.gene60722 | 0.828411 | 9.13E-55 | -1.69E-46 |
| MS.gene052089 | MS.gene60722 | 0.803977 | 2.69E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene60722 | -0.820749 | 5.82E-53 | -1.69E-46 |
| MS.gene052486 | MS.gene60722 | -0.816907 | 4.34E-52 | -1.69E-46 |
| MS.gene052566 | MS.gene60722 | 0.812889 | 3.38E-51 | -1.69E-46 |
| MS.gene052802 | MS.gene60722 | 0.813505 | 2.47E-51 | -1.69E-46 |
| MS.gene053069 | MS.gene60722 | 0.827379 | 1.62E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene60722 | -0.801675 | 8.05E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene60722 | 0.849283 | 3.57E-60 | -1.69E-46 |
| MS.gene053488 | MS.gene60722 | 0.835793 | 1.36E-56 | -1.69E-46 |
| MS.gene053549 | MS.gene60722 | 0.827044 | 1.94E-54 | -1.69E-46 |
| MS.gene053561 | MS.gene60722 | 0.857214 | 1.90E-62 | -1.69E-46 |
| MS.gene053826 | MS.gene60722 | 0.844586 | 6.90E-59 | -1.69E-46 |
| MS.gene054054 | MS.gene60722 | 0.816716 | 4.79E-52 | -1.69E-46 |
| MS.gene054056 | MS.gene60722 | 0.835925 | 1.26E-56 | -1.69E-46 |
| MS.gene054616 | MS.gene60722 | 0.802856 | 4.60E-49 | -1.69E-46 |
| MS.gene054707 | MS.gene60722 | 0.813788 | 2.14E-51 | -1.69E-46 |
| MS.gene054708 | MS.gene60722 | 0.844839 | 5.90E-59 | -1.69E-46 |
| MS.gene054954 | MS.gene60722 | -0.803203 | 3.90E-49 | -1.69E-46 |
| MS.gene055124 | MS.gene60722 | 0.846746 | 1.79E-59 | -1.69E-46 |
| MS.gene055139 | MS.gene60722 | 0.822389 | 2.43E-53 | -1.69E-46 |
| MS.gene055154 | MS.gene60722 | 0.868948 | 4.47E-66 | -1.69E-46 |
| MS.gene055202 | MS.gene60722 | 0.817345 | 3.46E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene60722 | -0.81004 | 1.40E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene60722 | -0.823988 | 1.03E-53 | -1.69E-46 |
| MS.gene055549 | MS.gene60722 | 0.85197 | 6.27E-61 | -1.69E-46 |
| MS.gene055550 | MS.gene60722 | 0.849505 | 3.10E-60 | -1.69E-46 |
| MS.gene055551 | MS.gene60722 | 0.823898 | 1.08E-53 | -1.69E-46 |
| MS.gene055750 | MS.gene60722 | -0.800124 | 1.67E-48 | -1.69E-46 |
| MS.gene05589 | MS.gene60722 | -0.818212 | 2.20E-52 | -1.69E-46 |
| MS.gene055942 | MS.gene60722 | 0.826381 | 2.80E-54 | -1.69E-46 |
| MS.gene055961 | MS.gene60722 | 0.810624 | 1.05E-50 | -1.69E-46 |
| MS.gene056224 | MS.gene60722 | 0.820885 | 5.41E-53 | -1.69E-46 |
| MS.gene056310 | MS.gene60722 | 0.880195 | 6.70E-70 | -1.69E-46 |
| MS.gene056311 | MS.gene60722 | 0.919222 | 5.90E-87 | -1.69E-46 |
| MS.gene056313 | MS.gene60722 | 0.863479 | 2.42E-64 | -1.69E-46 |
| MS.gene056365 | MS.gene60722 | 0.807572 | 4.73E-50 | -1.69E-46 |
| MS.gene056408 | MS.gene60722 | 0.808371 | 3.20E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene60722 | -0.83672 | 7.93E-57 | -1.69E-46 |
| MS.gene056530 | MS.gene60722 | 0.822765 | 1.99E-53 | -1.69E-46 |
| MS.gene057014 | MS.gene60722 | 0.892714 | 1.23E-74 | -1.69E-46 |
| MS.gene058068 | MS.gene60722 | 0.812427 | 4.26E-51 | -1.69E-46 |
| MS.gene058093 | MS.gene60722 | 0.800462 | 1.42E-48 | -1.69E-46 |
| MS.gene058099 | MS.gene60722 | 0.857809 | 1.27E-62 | -1.69E-46 |
| MS.gene058108 | MS.gene60722 | 0.835632 | 1.50E-56 | -1.69E-46 |
| MS.gene058109 | MS.gene60722 | 0.879657 | 1.04E-69 | -1.69E-46 |
| MS.gene058111 | MS.gene60722 | 0.903385 | 3.66E-79 | -1.69E-46 |
| MS.gene058117 | MS.gene60722 | 0.812446 | 4.22E-51 | -1.69E-46 |
| MS.gene058171 | MS.gene60722 | 0.891651 | 3.27E-74 | -1.69E-46 |
| MS.gene058173 | MS.gene60722 | 0.891242 | 4.75E-74 | -1.69E-46 |
| MS.gene058175 | MS.gene60722 | 0.895805 | 6.76E-76 | -1.69E-46 |
| MS.gene058268 | MS.gene60722 | 0.815892 | 7.33E-52 | -1.69E-46 |
| MS.gene058493 | MS.gene60722 | 0.874934 | 4.58E-68 | -1.69E-46 |
| MS.gene058494 | MS.gene60722 | 0.871771 | 5.30E-67 | -1.69E-46 |
| MS.gene058496 | MS.gene60722 | 0.828972 | 6.68E-55 | -1.69E-46 |
| MS.gene058628 | MS.gene60722 | 0.870897 | 1.03E-66 | -1.69E-46 |
| MS.gene058629 | MS.gene60722 | 0.859758 | 3.31E-63 | -1.69E-46 |
| MS.gene058630 | MS.gene60722 | 0.865017 | 8.01E-65 | -1.69E-46 |
| MS.gene05884 | MS.gene60722 | 0.814648 | 1.38E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene60722 | -0.832035 | 1.19E-55 | -1.69E-46 |
| MS.gene059045 | MS.gene60722 | 0.826665 | 2.40E-54 | -1.69E-46 |
| MS.gene059223 | MS.gene60722 | 0.838062 | 3.59E-57 | -1.69E-46 |
| MS.gene059690 | MS.gene60722 | 0.865085 | 7.63E-65 | -1.69E-46 |
| MS.gene059691 | MS.gene60722 | 0.837305 | 5.62E-57 | -1.69E-46 |
| MS.gene059694 | MS.gene60722 | 0.869183 | 3.75E-66 | -1.69E-46 |
| MS.gene059697 | MS.gene60722 | 0.870184 | 1.77E-66 | -1.69E-46 |
| MS.gene059865 | MS.gene60722 | -0.83317 | 6.22E-56 | -1.69E-46 |
| MS.gene059916 | MS.gene60722 | 0.805356 | 1.39E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene60722 | 0.813338 | 2.69E-51 | -1.69E-46 |
| MS.gene060778 | MS.gene60722 | -0.802487 | 5.48E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene60722 | -0.836877 | 7.23E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60722.t1 | MTR_1g097935 | 99.743 | 389 | 1 | 0 | 1 | 389 | 1 | 389 | 0.0 | 803 |
| MS.gene60722.t1 | MTR_1g097910 | 99.486 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 0.0 | 803 |
| MS.gene60722.t1 | MTR_1g098140 | 99.486 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 0.0 | 801 |
| MS.gene60722.t1 | MTR_7g016800 | 98.201 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene60722.t1 | MTR_7g016720 | 98.201 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 0.0 | 789 |
| MS.gene60722.t1 | MTR_7g016820 | 97.943 | 389 | 8 | 0 | 1 | 389 | 1 | 389 | 0.0 | 789 |
| MS.gene60722.t1 | MTR_5g007730 | 97.429 | 389 | 10 | 0 | 1 | 389 | 1 | 389 | 0.0 | 788 |
| MS.gene60722.t1 | MTR_7g016700 | 97.429 | 389 | 10 | 0 | 1 | 389 | 1 | 389 | 0.0 | 788 |
| MS.gene60722.t1 | MTR_7g016780 | 97.429 | 389 | 10 | 0 | 1 | 389 | 1 | 389 | 0.0 | 785 |
| MS.gene60722.t1 | MTR_5g007760 | 97.172 | 389 | 11 | 0 | 1 | 389 | 1 | 389 | 0.0 | 785 |
| MS.gene60722.t1 | MTR_5g007713 | 96.144 | 389 | 15 | 0 | 1 | 389 | 1 | 389 | 0.0 | 779 |
| MS.gene60722.t1 | MTR_5g007717 | 95.887 | 389 | 16 | 0 | 1 | 389 | 1 | 389 | 0.0 | 777 |
| MS.gene60722.t1 | MTR_3g083920 | 90.464 | 388 | 37 | 0 | 1 | 388 | 1 | 388 | 0.0 | 746 |
| MS.gene60722.t1 | MTR_3g083910 | 89.691 | 388 | 40 | 0 | 1 | 388 | 1 | 388 | 0.0 | 743 |
| MS.gene60722.t1 | MTR_2g058470 | 86.889 | 389 | 51 | 0 | 1 | 389 | 1 | 389 | 0.0 | 712 |
| MS.gene60722.t1 | MTR_3g086260 | 81.443 | 388 | 72 | 0 | 1 | 388 | 1 | 388 | 0.0 | 674 |
| MS.gene60722.t1 | MTR_7g084300 | 79.949 | 389 | 78 | 0 | 1 | 389 | 1 | 389 | 0.0 | 668 |
| MS.gene60722.t1 | MTR_7g113405 | 96.040 | 303 | 12 | 0 | 87 | 389 | 1 | 303 | 0.0 | 598 |
| MS.gene60722.t1 | MTR_3g088675 | 66.324 | 389 | 131 | 0 | 1 | 389 | 1 | 389 | 0.0 | 575 |
| MS.gene60722.t1 | MTR_5g007740 | 80.061 | 326 | 65 | 0 | 64 | 389 | 1 | 326 | 0.0 | 547 |
| MS.gene60722.t1 | MTR_5g007723 | 68.514 | 397 | 76 | 5 | 12 | 389 | 32 | 398 | 0.0 | 530 |
| MS.gene60722.t1 | MTR_5g007770 | 77.667 | 300 | 34 | 2 | 70 | 369 | 1 | 267 | 1.56e-166 | 467 |
| MS.gene60722.t1 | MTR_3g088670 | 48.718 | 390 | 186 | 6 | 10 | 388 | 2 | 388 | 7.02e-129 | 376 |
| MS.gene60722.t1 | MTR_4g075560 | 42.105 | 380 | 213 | 4 | 11 | 387 | 13 | 388 | 3.05e-100 | 303 |
| MS.gene60722.t1 | MTR_4g078730 | 37.802 | 373 | 225 | 2 | 15 | 387 | 24 | 389 | 5.87e-93 | 284 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60722.t1 | AT5G13930 | 82.216 | 388 | 68 | 1 | 3 | 389 | 8 | 395 | 0.0 | 669 |
| MS.gene60722.t1 | AT4G34850 | 40.849 | 377 | 219 | 3 | 15 | 389 | 18 | 392 | 1.21e-96 | 294 |
| MS.gene60722.t1 | AT4G00040 | 40.584 | 377 | 214 | 5 | 14 | 387 | 16 | 385 | 6.58e-95 | 289 |
| MS.gene60722.t1 | AT1G02050 | 39.894 | 376 | 216 | 5 | 15 | 387 | 24 | 392 | 4.75e-87 | 269 |
| MS.gene60722.t1 | AT4G34850 | 40.000 | 320 | 189 | 2 | 72 | 389 | 1 | 319 | 1.48e-80 | 250 |
Find 88 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGGTTTCTCAATTTC+TGG | 0.101255 | 1.2:+71213449 | None:intergenic |
| AGAAGGCCCTGCAACTATTT+TGG | 0.216290 | 1.2:-71214220 | MS.gene60722:CDS |
| CTGATTACAATTCAATCTTT+TGG | 0.254027 | 1.2:-71213259 | MS.gene60722:CDS |
| TACCAACAAGGTTGCTTTGC+AGG | 0.283953 | 1.2:-71213659 | MS.gene60722:CDS |
| CTTTCAAAATCTCCTCTGTT+AGG | 0.305425 | 1.2:+71213924 | None:intergenic |
| TCAATGGCCCCTTCACTATC+TGG | 0.309674 | 1.2:+71213395 | None:intergenic |
| CTTGTTGGACAAGCACTATT+TGG | 0.314421 | 1.2:-71213512 | MS.gene60722:CDS |
| AGAGAAGTGCTCAGTGAATA+TGG | 0.317887 | 1.2:-71213155 | MS.gene60722:CDS |
| GCTTCGTTTGGCTAAAGATT+TGG | 0.342769 | 1.2:-71213627 | MS.gene60722:CDS |
| TCTACTTGATCTAGAATTGC+AGG | 0.346751 | 1.2:+71213218 | None:intergenic |
| TGAATACATGGCACCTTCAT+TGG | 0.347646 | 1.2:-71213885 | MS.gene60722:CDS |
| GGTTTCTCAATTTCTGGTAC+TGG | 0.362281 | 1.2:+71213455 | None:intergenic |
| CCAATGGCCAAAATAGTTGC+AGG | 0.368786 | 1.2:+71214213 | None:intergenic |
| AAGTGAGTAATCTTTGACTT+TGG | 0.381814 | 1.2:+71213779 | None:intergenic |
| AGGAGGCACGGTGCTTCGTT+TGG | 0.381968 | 1.2:-71213639 | MS.gene60722:CDS |
| TACCAACTCACAAAACTCTT+AGG | 0.382277 | 1.2:-71213713 | MS.gene60722:CDS |
| CACCTTCTTAAAGATGTTCC+CGG | 0.401953 | 1.2:-71213341 | MS.gene60722:CDS |
| ATATTCACTGAGCACTTCTC+TGG | 0.410278 | 1.2:+71213157 | None:intergenic |
| GGAGCTGCTGCACTGATTGT+TGG | 0.411529 | 1.2:-71213485 | MS.gene60722:CDS |
| ACTCACTTGGACAGCCTTGT+TGG | 0.426712 | 1.2:-71213527 | MS.gene60722:CDS |
| ACGGGAGAAGGACTTGAGTT+TGG | 0.429869 | 1.2:-71213056 | MS.gene60722:CDS |
| AGTGAAAGCTATAAAAGAAT+GGG | 0.430526 | 1.2:-71213807 | MS.gene60722:CDS |
| GATAGTGAAGGGGCCATTGA+TGG | 0.440309 | 1.2:-71213392 | MS.gene60722:CDS |
| GTCACTGCAGTTACATTTCG+CGG | 0.450823 | 1.2:-71213560 | MS.gene60722:CDS |
| GCGCAAAACAACGGTTTCAA+TGG | 0.461439 | 1.2:+71213004 | None:intergenic |
| CTTTGTTCAACACAATTGGC+TGG | 0.464913 | 1.2:+71214180 | None:intergenic |
| CTGAAATTCGCAATGCTCAA+AGG | 0.468723 | 1.2:-71214245 | MS.gene60722:CDS |
| TTCACGAAGGTGACCATCAA+TGG | 0.470504 | 1.2:+71213379 | None:intergenic |
| GCAAGACATGGTGGTGGTAG+AGG | 0.474656 | 1.2:-71213855 | MS.gene60722:CDS |
| CTCCTGCAAAGCAACCTTGT+TGG | 0.478750 | 1.2:+71213657 | None:intergenic |
| GTGAGTTGGTAATCAGCTCC+AGG | 0.479782 | 1.2:+71213725 | None:intergenic |
| CAGTGAAAGCTATAAAAGAA+TGG | 0.482912 | 1.2:-71213808 | MS.gene60722:CDS |
| CTGTCCAAGTGAGTATCACT+AGG | 0.483602 | 1.2:+71213536 | None:intergenic |
| AAAGAACATCAATAAAGCAT+TGG | 0.485622 | 1.2:-71213309 | MS.gene60722:CDS |
| GATGGTCACCTTCGTGAAGC+TGG | 0.486168 | 1.2:-71213374 | MS.gene60722:CDS |
| TGTGCTTTGTTCAACACAAT+TGG | 0.494990 | 1.2:+71214176 | None:intergenic |
| TGAAATTCGCAATGCTCAAA+GGG | 0.495386 | 1.2:-71214244 | MS.gene60722:CDS |
| TCTAGAATTGCAGGTCCACC+AGG | 0.500155 | 1.2:+71213227 | None:intergenic |
| TTGCGCAGTGTCGCTATATG+AGG | 0.502378 | 1.2:-71212987 | None:intergenic |
| GTCTTCGCCCATATGTGAAA+AGG | 0.504788 | 1.2:-71213691 | MS.gene60722:CDS |
| TGGCACCTTCATTGGATGCT+AGG | 0.506096 | 1.2:-71213877 | MS.gene60722:CDS |
| GATGTTCTTTGAAACAATCC+CGG | 0.506109 | 1.2:+71213322 | None:intergenic |
| TTCACTGAGCACTTCTCTGG+TGG | 0.523654 | 1.2:+71213160 | None:intergenic |
| GGACTGAAGACCACGGGAGA+AGG | 0.524843 | 1.2:-71213068 | MS.gene60722:CDS |
| TCCTAGTGTTTGTGAATACA+TGG | 0.528394 | 1.2:-71213897 | MS.gene60722:CDS |
| CGGCCCTAGTGATACTCACT+TGG | 0.529910 | 1.2:-71213540 | MS.gene60722:CDS |
| CATCAATAAAGCATTGGTTG+AGG | 0.540608 | 1.2:-71213303 | MS.gene60722:CDS |
| ACCTTCTTAAAGATGTTCCC+GGG | 0.543150 | 1.2:-71213340 | MS.gene60722:CDS |
| ATGTTCTTTGAAACAATCCC+GGG | 0.547822 | 1.2:+71213323 | None:intergenic |
| CGCAATGCTCAAAGGGCAGA+AGG | 0.548900 | 1.2:-71214237 | MS.gene60722:CDS |
| AGGTTGCTTTGCAGGAGGCA+CGG | 0.555669 | 1.2:-71213651 | MS.gene60722:CDS |
| AGAAAGAAGTCAGCTCAAGA+TGG | 0.559235 | 1.2:-71213089 | MS.gene60722:CDS |
| GGACAAGCACTATTTGGAGA+TGG | 0.563405 | 1.2:-71213506 | MS.gene60722:CDS |
| GGCTGGATTTGCAGTGCCAA+TGG | 0.564489 | 1.2:+71214197 | None:intergenic |
| GCAGCCTCCTTGCCTAGTCT+AGG | 0.568229 | 1.2:+71213830 | None:intergenic |
| TCCCGGGAACATCTTTAAGA+AGG | 0.569831 | 1.2:+71213339 | None:intergenic |
| AGGCATGTCTACACCACTTG+TGG | 0.569916 | 1.2:+71213745 | None:intergenic |
| ACAAGTGGTGTAGACATGCC+TGG | 0.572074 | 1.2:-71213743 | MS.gene60722:CDS |
| CAAATAGTGCTTGTCCAACA+AGG | 0.572762 | 1.2:+71213513 | None:intergenic |
| AGCGACACTGCGCAAAACAA+CGG | 0.575560 | 1.2:+71212995 | None:intergenic |
| TCTTGCCTAGCATCCAATGA+AGG | 0.579296 | 1.2:+71213872 | None:intergenic |
| GTGATAAATCTATGATCAAG+AGG | 0.584564 | 1.2:-71213958 | MS.gene60722:CDS |
| CAATTGCTCCAGATAGTGAA+GGG | 0.593639 | 1.2:-71213403 | MS.gene60722:CDS |
| TTGATCGTTTGCACCACAAG+TGG | 0.595788 | 1.2:-71213758 | MS.gene60722:CDS |
| AGAGGTACCTAGACTAGGCA+AGG | 0.600359 | 1.2:-71213837 | MS.gene60722:CDS |
| CAAGATGGACTGAAGACCAC+GGG | 0.604373 | 1.2:-71213074 | MS.gene60722:CDS |
| ACAATTGCTCCAGATAGTGA+AGG | 0.608112 | 1.2:-71213404 | MS.gene60722:CDS |
| TTGTAATCAGAAATTCCCAA+TGG | 0.619803 | 1.2:+71213272 | None:intergenic |
| CAACAAAGGTGCTCGTGTGT+TGG | 0.620198 | 1.2:-71213597 | MS.gene60722:CDS |
| GTGGTAGAGGTACCTAGACT+AGG | 0.623290 | 1.2:-71213842 | MS.gene60722:CDS |
| CAACAAGGTTGCTTTGCAGG+AGG | 0.634702 | 1.2:-71213656 | MS.gene60722:CDS |
| AAACTCAAGTCCTTCTCCCG+TGG | 0.634843 | 1.2:+71213058 | None:intergenic |
| ATTGGATGCTAGGCAAGACA+TGG | 0.648366 | 1.2:-71213867 | MS.gene60722:CDS |
| CAATGGCCAAAATAGTTGCA+GGG | 0.663320 | 1.2:+71214214 | None:intergenic |
| GTGAAAGCTATAAAAGAATG+GGG | 0.667253 | 1.2:-71213806 | MS.gene60722:CDS |
| GAGATACATGTACCTAACAG+AGG | 0.667740 | 1.2:-71213936 | MS.gene60722:CDS |
| TCAAGATGGACTGAAGACCA+CGG | 0.668535 | 1.2:-71213075 | MS.gene60722:CDS |
| TGTCCAAGTGAGTATCACTA+GGG | 0.674277 | 1.2:+71213537 | None:intergenic |
| ACGGTTTCAATGGTAAGACC+CGG | 0.675121 | 1.2:+71213014 | None:intergenic |
| TGCTAGGCAAGACATGGTGG+TGG | 0.675229 | 1.2:-71213861 | MS.gene60722:CDS |
| GCCATGTATTCACAAACACT+AGG | 0.680230 | 1.2:+71213896 | None:intergenic |
| GATTTGGCTGAGAACAACAA+AGG | 0.683242 | 1.2:-71213611 | MS.gene60722:CDS |
| GGTACCTAGACTAGGCAAGG+AGG | 0.684618 | 1.2:-71213834 | MS.gene60722:CDS |
| ATGTTAGTCCAGCTTCACGA+AGG | 0.698828 | 1.2:+71213366 | None:intergenic |
| AATTGCTCCAGATAGTGAAG+GGG | 0.700404 | 1.2:-71213402 | MS.gene60722:CDS |
| AGGTATATGATGTACCAACA+AGG | 0.704257 | 1.2:-71213671 | MS.gene60722:CDS |
| CTAGAATTGCAGGTCCACCA+GGG | 0.711261 | 1.2:+71213228 | None:intergenic |
| GGATGCTAGGCAAGACATGG+TGG | 0.737305 | 1.2:-71213864 | MS.gene60722:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAGCAAAATATATTTAAA+TGG | + | chr1.2:71213204-71213223 | None:intergenic | 15.0% |
| !!! | ATATTTTGCTATCTTTACAT+TGG | - | chr1.2:71213211-71213230 | MS.gene60722:CDS | 20.0% |
| !!! | ATTTAAATGGAAAGTATCTA+AGG | + | chr1.2:71213191-71213210 | None:intergenic | 20.0% |
| ! | AAAATAGGTTTCTCAATTTC+TGG | + | chr1.2:71213801-71213820 | None:intergenic | 25.0% |
| ! | AAAGAACATCAATAAAGCAT+TGG | - | chr1.2:71213938-71213957 | MS.gene60722:CDS | 25.0% |
| ! | AGTGAAAGCTATAAAAGAAT+GGG | - | chr1.2:71213440-71213459 | MS.gene60722:CDS | 25.0% |
| !! | ATCATATACCTTTTCACATA+TGG | + | chr1.2:71213567-71213586 | None:intergenic | 25.0% |
| !! | CTGATTACAATTCAATCTTT+TGG | - | chr1.2:71213988-71214007 | MS.gene60722:intron | 25.0% |
| !! | TCATATACCTTTTCACATAT+GGG | + | chr1.2:71213566-71213585 | None:intergenic | 25.0% |
| !!! | GTGATTTTAAAGTAGAAATC+AGG | + | chr1.2:71213100-71213119 | None:intergenic | 25.0% |
| AAAAATACTTACACATGCGT+TGG | + | chr1.2:71213159-71213178 | None:intergenic | 30.0% | |
| ATCATAGATTTATCACCTGA+AGG | + | chr1.2:71213285-71213304 | None:intergenic | 30.0% | |
| CAGTGAAAGCTATAAAAGAA+TGG | - | chr1.2:71213439-71213458 | MS.gene60722:CDS | 30.0% | |
| GATATTAGTTCAGCAAACTT+GGG | + | chr1.2:71213247-71213266 | None:intergenic | 30.0% | |
| GTGAAAGCTATAAAAGAATG+GGG | - | chr1.2:71213441-71213460 | MS.gene60722:CDS | 30.0% | |
| GTGATAAATCTATGATCAAG+AGG | - | chr1.2:71213289-71213308 | MS.gene60722:CDS | 30.0% | |
| TCATAGATTTATCACCTGAA+GGG | + | chr1.2:71213284-71213303 | None:intergenic | 30.0% | |
| TGATATTAGTTCAGCAAACT+TGG | + | chr1.2:71213248-71213267 | None:intergenic | 30.0% | |
| TTGTAATCAGAAATTCCCAA+TGG | + | chr1.2:71213978-71213997 | None:intergenic | 30.0% | |
| ! | AAGTGAGTAATCTTTGACTT+TGG | + | chr1.2:71213471-71213490 | None:intergenic | 30.0% |
| ! | ATCTTTTCATAATTCCCTTC+AGG | - | chr1.2:71213267-71213286 | MS.gene60722:CDS | 30.0% |
| !! | TTTCATCTTTTCAGGCTTTA+AGG | + | chr1.2:71214066-71214085 | None:intergenic | 30.0% |
| !!! | AACCTATTTTTGAGATGGTT+TGG | - | chr1.2:71213811-71213830 | MS.gene60722:CDS | 30.0% |
| !!! | CTTGAGTTTGGTGTTTTATT+TGG | - | chr1.2:71214203-71214222 | MS.gene60722:CDS | 30.0% |
| !!! | TGAGAAACCTATTTTTGAGA+TGG | - | chr1.2:71213806-71213825 | MS.gene60722:CDS | 30.0% |
| !!! | TTTGGTGTTTTATTTGGCTT+TGG | - | chr1.2:71214209-71214228 | MS.gene60722:CDS | 30.0% |
| AGGTATATGATGTACCAACA+AGG | - | chr1.2:71213576-71213595 | MS.gene60722:CDS | 35.0% | |
| CTTTCAAAATCTCCTCTGTT+AGG | + | chr1.2:71213326-71213345 | None:intergenic | 35.0% | |
| GTCCAAACCATCTCAAAAAT+AGG | + | chr1.2:71213816-71213835 | None:intergenic | 35.0% | |
| TACCAACTCACAAAACTCTT+AGG | - | chr1.2:71213534-71213553 | MS.gene60722:CDS | 35.0% | |
| TCCTAGTGTTTGTGAATACA+TGG | - | chr1.2:71213350-71213369 | MS.gene60722:CDS | 35.0% | |
| TGAAATTCGCAATGCTCAAA+GGG | - | chr1.2:71213003-71213022 | MS.gene60722:CDS | 35.0% | |
| ! | TCTACTTGATCTAGAATTGC+AGG | + | chr1.2:71214032-71214051 | None:intergenic | 35.0% |
| ! | TGTGCTTTGTTCAACACAAT+TGG | + | chr1.2:71213074-71213093 | None:intergenic | 35.0% |
| !! | ATGGAAAGTATCTAAGGTAG+AGG | + | chr1.2:71213185-71213204 | None:intergenic | 35.0% |
| !! | ATGTTCTTTGAAACAATCCC+GGG | + | chr1.2:71213927-71213946 | None:intergenic | 35.0% |
| !! | CATCAATAAAGCATTGGTTG+AGG | - | chr1.2:71213944-71213963 | MS.gene60722:CDS | 35.0% |
| !! | GATGTTCTTTGAAACAATCC+CGG | + | chr1.2:71213928-71213947 | None:intergenic | 35.0% |
| !! | TGGAAAGTATCTAAGGTAGA+GGG | + | chr1.2:71213184-71213203 | None:intergenic | 35.0% |
| !!! | TGTTTTATTTGGCTTTGGAC+CGG | - | chr1.2:71214214-71214233 | MS.gene60722:CDS | 35.0% |
| AATTGCTCCAGATAGTGAAG+GGG | - | chr1.2:71213845-71213864 | MS.gene60722:CDS | 40.0% | |
| ACAATTGCTCCAGATAGTGA+AGG | - | chr1.2:71213843-71213862 | MS.gene60722:CDS | 40.0% | |
| ACCTTCTTAAAGATGTTCCC+GGG | - | chr1.2:71213907-71213926 | MS.gene60722:CDS | 40.0% | |
| AGAAAGAAGTCAGCTCAAGA+TGG | - | chr1.2:71214158-71214177 | MS.gene60722:CDS | 40.0% | |
| ATATTCACTGAGCACTTCTC+TGG | + | chr1.2:71214093-71214112 | None:intergenic | 40.0% | |
| CAAATAGTGCTTGTCCAACA+AGG | + | chr1.2:71213737-71213756 | None:intergenic | 40.0% | |
| CAATGGCCAAAATAGTTGCA+GGG | + | chr1.2:71213036-71213055 | None:intergenic | 40.0% | |
| CAATTGCTCCAGATAGTGAA+GGG | - | chr1.2:71213844-71213863 | MS.gene60722:CDS | 40.0% | |
| CACCTTCTTAAAGATGTTCC+CGG | - | chr1.2:71213906-71213925 | MS.gene60722:CDS | 40.0% | |
| CTGAAATTCGCAATGCTCAA+AGG | - | chr1.2:71213002-71213021 | MS.gene60722:CDS | 40.0% | |
| CTTGTTGGACAAGCACTATT+TGG | - | chr1.2:71213735-71213754 | MS.gene60722:CDS | 40.0% | |
| CTTTGTTCAACACAATTGGC+TGG | + | chr1.2:71213070-71213089 | None:intergenic | 40.0% | |
| GAGATACATGTACCTAACAG+AGG | - | chr1.2:71213311-71213330 | MS.gene60722:CDS | 40.0% | |
| GATTTGGCTGAGAACAACAA+AGG | - | chr1.2:71213636-71213655 | MS.gene60722:CDS | 40.0% | |
| GCCATGTATTCACAAACACT+AGG | + | chr1.2:71213354-71213373 | None:intergenic | 40.0% | |
| GCTTCGTTTGGCTAAAGATT+TGG | - | chr1.2:71213620-71213639 | MS.gene60722:CDS | 40.0% | |
| TGAATACATGGCACCTTCAT+TGG | - | chr1.2:71213362-71213381 | MS.gene60722:CDS | 40.0% | |
| TGTCCAAGTGAGTATCACTA+GGG | + | chr1.2:71213713-71213732 | None:intergenic | 40.0% | |
| ! | GTTGAGGCTTTTGAACCATT+GGG | - | chr1.2:71213960-71213979 | MS.gene60722:CDS | 40.0% |
| !! | AGAGAAGTGCTCAGTGAATA+TGG | - | chr1.2:71214092-71214111 | MS.gene60722:intron | 40.0% |
| !! | GACCTAAGAGTTTTGTGAGT+TGG | + | chr1.2:71213539-71213558 | None:intergenic | 40.0% |
| !! | GGTTTCTCAATTTCTGGTAC+TGG | + | chr1.2:71213795-71213814 | None:intergenic | 40.0% |
| !!! | GTTTTATTTGGCTTTGGACC+GGG | - | chr1.2:71214215-71214234 | MS.gene60722:CDS | 40.0% |
| ACGGTTTCAATGGTAAGACC+CGG | + | chr1.2:71214236-71214255 | None:intergenic | 45.0% | |
| ATGTTAGTCCAGCTTCACGA+AGG | + | chr1.2:71213884-71213903 | None:intergenic | 45.0% | |
| ATTGGATGCTAGGCAAGACA+TGG | - | chr1.2:71213380-71213399 | MS.gene60722:CDS | 45.0% | |
| CCAATGGCCAAAATAGTTGC+AGG | + | chr1.2:71213037-71213056 | None:intergenic | 45.0% | |
| CTGTCCAAGTGAGTATCACT+AGG | + | chr1.2:71213714-71213733 | None:intergenic | 45.0% | |
| GCGCAAAACAACGGTTTCAA+TGG | + | chr1.2:71214246-71214265 | None:intergenic | 45.0% | |
| GGACAAGCACTATTTGGAGA+TGG | - | chr1.2:71213741-71213760 | MS.gene60722:CDS | 45.0% | |
| GTCACTGCAGTTACATTTCG+CGG | - | chr1.2:71213687-71213706 | MS.gene60722:CDS | 45.0% | |
| GTCTTCGCCCATATGTGAAA+AGG | - | chr1.2:71213556-71213575 | MS.gene60722:CDS | 45.0% | |
| TCTTGCCTAGCATCCAATGA+AGG | + | chr1.2:71213378-71213397 | None:intergenic | 45.0% | |
| TTCACGAAGGTGACCATCAA+TGG | + | chr1.2:71213871-71213890 | None:intergenic | 45.0% | |
| TTGATCGTTTGCACCACAAG+TGG | - | chr1.2:71213489-71213508 | MS.gene60722:CDS | 45.0% | |
| ! | AGAAGGCCCTGCAACTATTT+TGG | - | chr1.2:71213027-71213046 | MS.gene60722:CDS | 45.0% |
| ! | ATCTTTTGGATTGCACACCC+TGG | - | chr1.2:71214002-71214021 | MS.gene60722:intron | 45.0% |
| ! | CTGGTGGCTTTCATCTTTTC+AGG | + | chr1.2:71214074-71214093 | None:intergenic | 45.0% |
| ! | GGTTGAGGCTTTTGAACCAT+TGG | - | chr1.2:71213959-71213978 | MS.gene60722:CDS | 45.0% |
| ! | TACCAACAAGGTTGCTTTGC+AGG | - | chr1.2:71213588-71213607 | MS.gene60722:CDS | 45.0% |
| ! | TCCCGGGAACATCTTTAAGA+AGG | + | chr1.2:71213911-71213930 | None:intergenic | 45.0% |
| !! | TCAAGATGGACTGAAGACCA+CGG | - | chr1.2:71214172-71214191 | MS.gene60722:CDS | 45.0% |
| !!! | CCTGCAACTATTTTGGCCAT+TGG | - | chr1.2:71213034-71213053 | MS.gene60722:CDS | 45.0% |
| AAACTCAAGTCCTTCTCCCG+TGG | + | chr1.2:71214192-71214211 | None:intergenic | 50.0% | |
| ACAAGTGGTGTAGACATGCC+TGG | - | chr1.2:71213504-71213523 | MS.gene60722:CDS | 50.0% | |
| ACGGGAGAAGGACTTGAGTT+TGG | - | chr1.2:71214191-71214210 | MS.gene60722:CDS | 50.0% | |
| AGCGACACTGCGCAAAACAA+CGG | + | chr1.2:71214255-71214274 | None:intergenic | 50.0% | |
| AGGCATGTCTACACCACTTG+TGG | + | chr1.2:71213505-71213524 | None:intergenic | 50.0% | |
| CTAGAATTGCAGGTCCACCA+GGG | + | chr1.2:71214022-71214041 | None:intergenic | 50.0% | |
| CTCCTGCAAAGCAACCTTGT+TGG | + | chr1.2:71213593-71213612 | None:intergenic | 50.0% | |
| GATAGTGAAGGGGCCATTGA+TGG | - | chr1.2:71213855-71213874 | MS.gene60722:CDS | 50.0% | |
| TCAATGGCCCCTTCACTATC+TGG | + | chr1.2:71213855-71213874 | None:intergenic | 50.0% | |
| TGGCACCTTCATTGGATGCT+AGG | - | chr1.2:71213370-71213389 | MS.gene60722:CDS | 50.0% | |
| TTCACTGAGCACTTCTCTGG+TGG | + | chr1.2:71214090-71214109 | None:intergenic | 50.0% | |
| ! | ACTCACTTGGACAGCCTTGT+TGG | - | chr1.2:71213720-71213739 | MS.gene60722:CDS | 50.0% |
| ! | CAACAAGGTTGCTTTGCAGG+AGG | - | chr1.2:71213591-71213610 | MS.gene60722:CDS | 50.0% |
| ! | TCTAGAATTGCAGGTCCACC+AGG | + | chr1.2:71214023-71214042 | None:intergenic | 50.0% |
| ! | TTTTGGATTGCACACCCTGG+TGG | - | chr1.2:71214005-71214024 | MS.gene60722:intron | 50.0% |
| !! | AGAGGTACCTAGACTAGGCA+AGG | - | chr1.2:71213410-71213429 | MS.gene60722:CDS | 50.0% |
| !! | CAACAAAGGTGCTCGTGTGT+TGG | - | chr1.2:71213650-71213669 | MS.gene60722:CDS | 50.0% |
| !! | CAAGATGGACTGAAGACCAC+GGG | - | chr1.2:71214173-71214192 | MS.gene60722:CDS | 50.0% |
| !! | GTGAGTTGGTAATCAGCTCC+AGG | + | chr1.2:71213525-71213544 | None:intergenic | 50.0% |
| !! | GTGGTAGAGGTACCTAGACT+AGG | - | chr1.2:71213405-71213424 | MS.gene60722:CDS | 50.0% |
| CGCAATGCTCAAAGGGCAGA+AGG | - | chr1.2:71213010-71213029 | MS.gene60722:CDS | 55.0% | |
| CGGCCCTAGTGATACTCACT+TGG | - | chr1.2:71213707-71213726 | MS.gene60722:CDS | 55.0% | |
| GATGGTCACCTTCGTGAAGC+TGG | - | chr1.2:71213873-71213892 | MS.gene60722:CDS | 55.0% | |
| GGATGCTAGGCAAGACATGG+TGG | - | chr1.2:71213383-71213402 | MS.gene60722:CDS | 55.0% | |
| GGCTGGATTTGCAGTGCCAA+TGG | + | chr1.2:71213053-71213072 | None:intergenic | 55.0% | |
| GGTACCTAGACTAGGCAAGG+AGG | - | chr1.2:71213413-71213432 | MS.gene60722:CDS | 55.0% | |
| TGCTAGGCAAGACATGGTGG+TGG | - | chr1.2:71213386-71213405 | MS.gene60722:CDS | 55.0% | |
| ! | AGGTTGCTTTGCAGGAGGCA+CGG | - | chr1.2:71213596-71213615 | MS.gene60722:CDS | 55.0% |
| !! | GCAAGACATGGTGGTGGTAG+AGG | - | chr1.2:71213392-71213411 | MS.gene60722:CDS | 55.0% |
| !! | GGAGCTGCTGCACTGATTGT+TGG | - | chr1.2:71213762-71213781 | MS.gene60722:CDS | 55.0% |
| GCAGCCTCCTTGCCTAGTCT+AGG | + | chr1.2:71213420-71213439 | None:intergenic | 60.0% | |
| GGACTGAAGACCACGGGAGA+AGG | - | chr1.2:71214179-71214198 | MS.gene60722:CDS | 60.0% | |
| ! | AGGAGGCACGGTGCTTCGTT+TGG | - | chr1.2:71213608-71213627 | MS.gene60722:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 71212989 | 71214280 | 71212989 | ID=MS.gene60722 |
| chr1.2 | mRNA | 71212989 | 71214280 | 71212989 | ID=MS.gene60722.t1;Parent=MS.gene60722 |
| chr1.2 | exon | 71214103 | 71214280 | 71214103 | ID=MS.gene60722.t1.exon1;Parent=MS.gene60722.t1 |
| chr1.2 | CDS | 71214103 | 71214280 | 71214103 | ID=cds.MS.gene60722.t1;Parent=MS.gene60722.t1 |
| chr1.2 | exon | 71212989 | 71213980 | 71212989 | ID=MS.gene60722.t1.exon2;Parent=MS.gene60722.t1 |
| chr1.2 | CDS | 71212989 | 71213980 | 71212989 | ID=cds.MS.gene60722.t1;Parent=MS.gene60722.t1 |
| Gene Sequence |
| Protein sequence |
