Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60720.t1 | XP_003592044.1 | 99.5 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 1.50E-221 | 778.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60720.t1 | P30075 | 98.2 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 9.7e-222 | 770.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60720.t1 | G7IAK9 | 99.5 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 1.1e-221 | 778.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049281 | MS.gene60720 | 0.818838 | 1.59E-52 | -1.69E-46 |
| MS.gene049312 | MS.gene60720 | 0.817289 | 3.56E-52 | -1.69E-46 |
| MS.gene050363 | MS.gene60720 | 0.830446 | 2.92E-55 | -1.69E-46 |
| MS.gene050381 | MS.gene60720 | 0.877136 | 8.00E-69 | -1.69E-46 |
| MS.gene050504 | MS.gene60720 | -0.808605 | 2.85E-50 | -1.69E-46 |
| MS.gene050612 | MS.gene60720 | -0.80674 | 7.10E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene60720 | -0.804221 | 2.40E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene60720 | -0.804776 | 1.84E-49 | -1.69E-46 |
| MS.gene050883 | MS.gene60720 | 0.931359 | 4.24E-94 | -1.69E-46 |
| MS.gene051044 | MS.gene60720 | 0.849418 | 3.28E-60 | -1.69E-46 |
| MS.gene051127 | MS.gene60720 | 0.853544 | 2.23E-61 | -1.69E-46 |
| MS.gene051364 | MS.gene60720 | 0.802878 | 4.55E-49 | -1.69E-46 |
| MS.gene05141 | MS.gene60720 | 0.823226 | 1.55E-53 | -1.69E-46 |
| MS.gene051946 | MS.gene60720 | 0.859883 | 3.04E-63 | -1.69E-46 |
| MS.gene052486 | MS.gene60720 | -0.800702 | 1.27E-48 | -1.69E-46 |
| MS.gene053069 | MS.gene60720 | 0.804902 | 1.73E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene60720 | 0.810194 | 1.30E-50 | -1.69E-46 |
| MS.gene053488 | MS.gene60720 | 0.815441 | 9.23E-52 | -1.69E-46 |
| MS.gene053549 | MS.gene60720 | 0.833733 | 4.50E-56 | -1.69E-46 |
| MS.gene053561 | MS.gene60720 | 0.833144 | 6.31E-56 | -1.69E-46 |
| MS.gene053826 | MS.gene60720 | 0.839019 | 2.03E-57 | -1.69E-46 |
| MS.gene054054 | MS.gene60720 | 0.807078 | 6.03E-50 | -1.69E-46 |
| MS.gene054056 | MS.gene60720 | 0.824987 | 5.99E-54 | -1.69E-46 |
| MS.gene054708 | MS.gene60720 | 0.83746 | 5.13E-57 | -1.69E-46 |
| MS.gene055124 | MS.gene60720 | 0.851808 | 6.97E-61 | -1.69E-46 |
| MS.gene055139 | MS.gene60720 | 0.822335 | 2.50E-53 | -1.69E-46 |
| MS.gene055154 | MS.gene60720 | 0.858426 | 8.31E-63 | -1.69E-46 |
| MS.gene055202 | MS.gene60720 | 0.808509 | 2.99E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene60720 | -0.807331 | 5.33E-50 | -1.69E-46 |
| MS.gene055549 | MS.gene60720 | 0.834766 | 2.48E-56 | -1.69E-46 |
| MS.gene055550 | MS.gene60720 | 0.837233 | 5.86E-57 | -1.69E-46 |
| MS.gene055551 | MS.gene60720 | 0.818252 | 2.16E-52 | -1.69E-46 |
| MS.gene05589 | MS.gene60720 | -0.802794 | 4.74E-49 | -1.69E-46 |
| MS.gene055942 | MS.gene60720 | 0.803449 | 3.47E-49 | -1.69E-46 |
| MS.gene055961 | MS.gene60720 | 0.818147 | 2.28E-52 | -1.69E-46 |
| MS.gene056310 | MS.gene60720 | 0.882435 | 1.04E-70 | -1.69E-46 |
| MS.gene056311 | MS.gene60720 | 0.906366 | 1.60E-80 | -1.69E-46 |
| MS.gene056313 | MS.gene60720 | 0.867259 | 1.56E-65 | -1.69E-46 |
| MS.gene056408 | MS.gene60720 | 0.807869 | 4.09E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene60720 | -0.825593 | 4.31E-54 | -1.69E-46 |
| MS.gene056530 | MS.gene60720 | 0.81178 | 5.90E-51 | -1.69E-46 |
| MS.gene057014 | MS.gene60720 | 0.876346 | 1.50E-68 | -1.69E-46 |
| MS.gene058093 | MS.gene60720 | 0.808726 | 2.69E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene60720 | 0.841963 | 3.45E-58 | -1.69E-46 |
| MS.gene058108 | MS.gene60720 | 0.839876 | 1.22E-57 | -1.69E-46 |
| MS.gene058109 | MS.gene60720 | 0.887763 | 1.07E-72 | -1.69E-46 |
| MS.gene058111 | MS.gene60720 | 0.884572 | 1.71E-71 | -1.69E-46 |
| MS.gene058117 | MS.gene60720 | 0.800569 | 1.36E-48 | -1.69E-46 |
| MS.gene058171 | MS.gene60720 | 0.877641 | 5.34E-69 | -1.69E-46 |
| MS.gene058173 | MS.gene60720 | 0.873251 | 1.70E-67 | -1.69E-46 |
| MS.gene058175 | MS.gene60720 | 0.879279 | 1.42E-69 | -1.69E-46 |
| MS.gene058493 | MS.gene60720 | 0.850788 | 1.35E-60 | -1.69E-46 |
| MS.gene058494 | MS.gene60720 | 0.837192 | 6.01E-57 | -1.69E-46 |
| MS.gene058496 | MS.gene60720 | 0.804067 | 2.58E-49 | -1.69E-46 |
| MS.gene058628 | MS.gene60720 | 0.869797 | 2.37E-66 | -1.69E-46 |
| MS.gene058629 | MS.gene60720 | 0.859302 | 4.54E-63 | -1.69E-46 |
| MS.gene058630 | MS.gene60720 | 0.870921 | 1.01E-66 | -1.69E-46 |
| MS.gene059022 | MS.gene60720 | -0.800697 | 1.28E-48 | -1.69E-46 |
| MS.gene059045 | MS.gene60720 | 0.830553 | 2.75E-55 | -1.69E-46 |
| MS.gene059223 | MS.gene60720 | 0.817751 | 2.80E-52 | -1.69E-46 |
| MS.gene059690 | MS.gene60720 | 0.860801 | 1.60E-63 | -1.69E-46 |
| MS.gene059691 | MS.gene60720 | 0.842482 | 2.52E-58 | -1.69E-46 |
| MS.gene059694 | MS.gene60720 | 0.86981 | 2.34E-66 | -1.69E-46 |
| MS.gene059697 | MS.gene60720 | 0.873403 | 1.51E-67 | -1.69E-46 |
| MS.gene059865 | MS.gene60720 | -0.813475 | 2.51E-51 | -1.69E-46 |
| MS.gene059916 | MS.gene60720 | 0.808176 | 3.52E-50 | -1.69E-46 |
| MS.gene060595 | MS.gene60720 | 0.801309 | 9.57E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene60720 | -0.819298 | 1.25E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60720.t1 | MTR_1g098140 | 99.486 | 389 | 2 | 0 | 1 | 389 | 1 | 389 | 0.0 | 803 |
| MS.gene60720.t1 | MTR_1g097935 | 99.229 | 389 | 3 | 0 | 1 | 389 | 1 | 389 | 0.0 | 801 |
| MS.gene60720.t1 | MTR_1g097910 | 98.972 | 389 | 4 | 0 | 1 | 389 | 1 | 389 | 0.0 | 801 |
| MS.gene60720.t1 | MTR_7g016800 | 98.715 | 389 | 5 | 0 | 1 | 389 | 1 | 389 | 0.0 | 798 |
| MS.gene60720.t1 | MTR_7g016820 | 97.943 | 389 | 8 | 0 | 1 | 389 | 1 | 389 | 0.0 | 792 |
| MS.gene60720.t1 | MTR_7g016700 | 97.943 | 389 | 8 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene60720.t1 | MTR_7g016720 | 98.201 | 389 | 7 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene60720.t1 | MTR_5g007730 | 97.429 | 389 | 10 | 0 | 1 | 389 | 1 | 389 | 0.0 | 791 |
| MS.gene60720.t1 | MTR_5g007760 | 97.686 | 389 | 9 | 0 | 1 | 389 | 1 | 389 | 0.0 | 790 |
| MS.gene60720.t1 | MTR_7g016780 | 97.429 | 389 | 10 | 0 | 1 | 389 | 1 | 389 | 0.0 | 788 |
| MS.gene60720.t1 | MTR_5g007713 | 96.658 | 389 | 13 | 0 | 1 | 389 | 1 | 389 | 0.0 | 783 |
| MS.gene60720.t1 | MTR_5g007717 | 96.401 | 389 | 14 | 0 | 1 | 389 | 1 | 389 | 0.0 | 782 |
| MS.gene60720.t1 | MTR_3g083920 | 90.979 | 388 | 35 | 0 | 1 | 388 | 1 | 388 | 0.0 | 751 |
| MS.gene60720.t1 | MTR_3g083910 | 90.181 | 387 | 38 | 0 | 1 | 387 | 1 | 387 | 0.0 | 746 |
| MS.gene60720.t1 | MTR_2g058470 | 87.404 | 389 | 49 | 0 | 1 | 389 | 1 | 389 | 0.0 | 716 |
| MS.gene60720.t1 | MTR_3g086260 | 81.912 | 387 | 70 | 0 | 1 | 387 | 1 | 387 | 0.0 | 679 |
| MS.gene60720.t1 | MTR_7g084300 | 80.463 | 389 | 76 | 0 | 1 | 389 | 1 | 389 | 0.0 | 674 |
| MS.gene60720.t1 | MTR_7g113405 | 95.710 | 303 | 13 | 0 | 87 | 389 | 1 | 303 | 0.0 | 598 |
| MS.gene60720.t1 | MTR_3g088675 | 66.324 | 389 | 131 | 0 | 1 | 389 | 1 | 389 | 0.0 | 577 |
| MS.gene60720.t1 | MTR_5g007740 | 80.061 | 326 | 65 | 0 | 64 | 389 | 1 | 326 | 0.0 | 549 |
| MS.gene60720.t1 | MTR_5g007723 | 69.018 | 397 | 74 | 5 | 12 | 389 | 32 | 398 | 0.0 | 534 |
| MS.gene60720.t1 | MTR_5g007770 | 77.667 | 300 | 34 | 2 | 70 | 369 | 1 | 267 | 1.77e-167 | 469 |
| MS.gene60720.t1 | MTR_3g088670 | 49.100 | 389 | 184 | 6 | 10 | 387 | 2 | 387 | 2.42e-130 | 380 |
| MS.gene60720.t1 | MTR_4g075560 | 42.632 | 380 | 211 | 4 | 11 | 387 | 13 | 388 | 1.51e-102 | 309 |
| MS.gene60720.t1 | MTR_4g078730 | 37.802 | 373 | 225 | 2 | 15 | 387 | 24 | 389 | 7.12e-94 | 286 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60720.t1 | AT5G13930 | 82.732 | 388 | 66 | 1 | 3 | 389 | 8 | 395 | 0.0 | 675 |
| MS.gene60720.t1 | AT4G34850 | 40.849 | 377 | 219 | 3 | 15 | 389 | 18 | 392 | 1.30e-97 | 296 |
| MS.gene60720.t1 | AT4G00040 | 40.584 | 377 | 214 | 5 | 14 | 387 | 16 | 385 | 1.30e-95 | 291 |
| MS.gene60720.t1 | AT1G02050 | 40.053 | 377 | 216 | 5 | 15 | 388 | 24 | 393 | 1.64e-88 | 273 |
| MS.gene60720.t1 | AT4G34850 | 40.312 | 320 | 188 | 2 | 72 | 389 | 1 | 319 | 4.31e-82 | 254 |
| MS.gene60720.t1 | AT3G52160 | 26.749 | 243 | 158 | 9 | 150 | 387 | 97 | 324 | 3.51e-12 | 67.4 |
| MS.gene60720.t1 | AT3G52160 | 26.749 | 243 | 158 | 8 | 150 | 387 | 210 | 437 | 8.67e-12 | 67.0 |
Find 91 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGGTTTCTCAATTTC+TGG | 0.101255 | 1.2:+71200923 | None:intergenic |
| TGCAGTTCCAATGGCTAAAA+TGG | 0.233615 | 1.2:+71201688 | None:intergenic |
| TGCAGGGCCTTCTGCCCTTT+GGG | 0.236458 | 1.2:+71201712 | None:intergenic |
| CTGATTACAATTCAATCTTT+TGG | 0.254027 | 1.2:-71200733 | MS.gene60720:CDS |
| TTGCAGGGCCTTCTGCCCTT+TGG | 0.271491 | 1.2:+71201711 | None:intergenic |
| GGTTTCTCAATTTCTGGTAT+TGG | 0.282073 | 1.2:+71200929 | None:intergenic |
| TACCAACAAGGTTGCTTTGC+AGG | 0.283953 | 1.2:-71201133 | MS.gene60720:CDS |
| AGAGAAGTGCTTAGTGAATA+TGG | 0.295286 | 1.2:-71200629 | MS.gene60720:CDS |
| CTTTCAAAATCTCCTCTGTT+AGG | 0.305425 | 1.2:+71201398 | None:intergenic |
| CCTTTGGGCATTGCGAATTT+CGG | 0.305637 | 1.2:+71201727 | None:intergenic |
| CTTGTTGGACAAGCACTATT+TGG | 0.314421 | 1.2:-71200986 | MS.gene60720:CDS |
| GCTTCGTTTGGCTAAAGATT+TGG | 0.342769 | 1.2:-71201101 | MS.gene60720:CDS |
| TGAATACATGGCACCTTCAT+TGG | 0.347646 | 1.2:-71201359 | MS.gene60720:CDS |
| TCAATGGCTCCTTCACTATC+TGG | 0.348362 | 1.2:+71200869 | None:intergenic |
| TCAACTTGATCTAGAATTGC+AGG | 0.376968 | 1.2:+71200692 | None:intergenic |
| AAGTGAGTAATCTTTGACTT+TGG | 0.381814 | 1.2:+71201253 | None:intergenic |
| AGGAGGCACGGTGCTTCGTT+TGG | 0.381968 | 1.2:-71201113 | MS.gene60720:CDS |
| TACCAACTCACAAAACTCTT+AGG | 0.382277 | 1.2:-71201187 | MS.gene60720:CDS |
| CTTTGTTCAACACAATTGGC+TGG | 0.390254 | 1.2:+71201662 | None:intergenic |
| CCAATGGCTAAAATGGTTGC+AGG | 0.393820 | 1.2:+71201695 | None:intergenic |
| CACCTTCTTAAAGATGTTCC+CGG | 0.401953 | 1.2:-71200815 | MS.gene60720:CDS |
| GGAGCTGCTGCACTGATTGT+TGG | 0.411529 | 1.2:-71200959 | MS.gene60720:CDS |
| ACTCACTTGGACAGCCTTGT+TGG | 0.426712 | 1.2:-71201001 | MS.gene60720:CDS |
| AGTGAAAGCTATAAAAGAAT+GGG | 0.430526 | 1.2:-71201281 | MS.gene60720:CDS |
| GTCACTGCAGTTACATTTCG+CGG | 0.450823 | 1.2:-71201034 | MS.gene60720:CDS |
| TTGCGTAGTGTCACTATATG+AGG | 0.451569 | 1.2:-71200461 | None:intergenic |
| GTGATAAATCTATGATCAAA+AGG | 0.453113 | 1.2:-71201432 | MS.gene60720:CDS |
| ACGCAAAACAACGGTTTCAA+TGG | 0.461439 | 1.2:+71200478 | None:intergenic |
| ATATTCACTAAGCACTTCTC+TGG | 0.467287 | 1.2:+71200631 | None:intergenic |
| TTCACGAAGGTGACCATCAA+TGG | 0.471758 | 1.2:+71200853 | None:intergenic |
| CTCCTGCAAAGCAACCTTGT+TGG | 0.478750 | 1.2:+71201131 | None:intergenic |
| GTGAGTTGGTAATCAGCTCC+AGG | 0.479782 | 1.2:+71201199 | None:intergenic |
| GATAGTGAAGGAGCCATTGA+TGG | 0.482535 | 1.2:-71200866 | MS.gene60720:CDS |
| CAGTGAAAGCTATAAAAGAA+TGG | 0.482912 | 1.2:-71201282 | MS.gene60720:CDS |
| CTGTCCAAGTGAGTATCACT+AGG | 0.483602 | 1.2:+71201010 | None:intergenic |
| AAAGAACATCAATAAAGCAT+TGG | 0.485622 | 1.2:-71200783 | MS.gene60720:CDS |
| GATGGTCACCTTCGTGAAGC+TGG | 0.486168 | 1.2:-71200848 | MS.gene60720:CDS |
| TGTGCTTTGTTCAACACAAT+TGG | 0.494990 | 1.2:+71201658 | None:intergenic |
| CGAAATTCGCAATGCCCAAA+GGG | 0.497687 | 1.2:-71201726 | MS.gene60720:CDS |
| TCTAGAATTGCAGGTCCACC+AGG | 0.500155 | 1.2:+71200701 | None:intergenic |
| GGCTGGGTTTGCAGTTCCAA+TGG | 0.502395 | 1.2:+71201679 | None:intergenic |
| GTCTTCGCCCATATGTGAAA+AGG | 0.504788 | 1.2:-71201165 | MS.gene60720:CDS |
| TGGCACCTTCATTGGATGCT+AGG | 0.506096 | 1.2:-71201351 | MS.gene60720:CDS |
| GATGTTCTTTGAAACAATCC+CGG | 0.506109 | 1.2:+71200796 | None:intergenic |
| GGACTGAAGACCACGGGAGA+AGG | 0.524843 | 1.2:-71200542 | MS.gene60720:CDS |
| TCCTAGTGTTTGTGAATACA+TGG | 0.528394 | 1.2:-71201371 | MS.gene60720:CDS |
| CGCAATGCCCAAAGGGCAGA+AGG | 0.529398 | 1.2:-71201719 | MS.gene60720:CDS |
| CGGCCCTAGTGATACTCACT+TGG | 0.529910 | 1.2:-71201014 | MS.gene60720:CDS |
| CCGAAATTCGCAATGCCCAA+AGG | 0.537611 | 1.2:-71201727 | MS.gene60720:CDS |
| TTTGTTCAACACAATTGGCT+GGG | 0.538613 | 1.2:+71201663 | None:intergenic |
| CATCAATAAAGCATTGGTTG+AGG | 0.540608 | 1.2:-71200777 | MS.gene60720:CDS |
| ACCTTCTTAAAGATGTTCCC+GGG | 0.543150 | 1.2:-71200814 | MS.gene60720:CDS |
| ATGTTCTTTGAAACAATCCC+GGG | 0.547822 | 1.2:+71200797 | None:intergenic |
| AGGTTGCTTTGCAGGAGGCA+CGG | 0.555669 | 1.2:-71201125 | MS.gene60720:CDS |
| AGAAAGAAGTCAGCTCAAGA+TGG | 0.559235 | 1.2:-71200563 | MS.gene60720:CDS |
| GGACAAGCACTATTTGGAGA+TGG | 0.563405 | 1.2:-71200980 | MS.gene60720:CDS |
| TTCACTAAGCACTTCTCTGG+TGG | 0.567752 | 1.2:+71200634 | None:intergenic |
| GCAGCCTCCTTGCCTAGTCT+AGG | 0.568229 | 1.2:+71201304 | None:intergenic |
| TCCCGGGAACATCTTTAAGA+AGG | 0.569831 | 1.2:+71200813 | None:intergenic |
| AGGCATGTCTACACCACTTG+TGG | 0.569916 | 1.2:+71201219 | None:intergenic |
| GCAAGACATGGTGGTTGTAG+AGG | 0.570536 | 1.2:-71201329 | MS.gene60720:CDS |
| ACAAGTGGTGTAGACATGCC+TGG | 0.572074 | 1.2:-71201217 | MS.gene60720:CDS |
| CACGGGAGAAGGACTTGAGT+GGG | 0.572386 | 1.2:-71200531 | MS.gene60720:CDS |
| CAAATAGTGCTTGTCCAACA+AGG | 0.572762 | 1.2:+71200987 | None:intergenic |
| TCTTGCCTAGCATCCAATGA+AGG | 0.579296 | 1.2:+71201346 | None:intergenic |
| TTGATCGTTTGCACCACAAG+TGG | 0.595788 | 1.2:-71201232 | MS.gene60720:CDS |
| CAAGATGGACTGAAGACCAC+GGG | 0.604373 | 1.2:-71200548 | MS.gene60720:CDS |
| CCACTCAAGTCCTTCTCCCG+TGG | 0.610390 | 1.2:+71200532 | None:intergenic |
| ACGGTTTCAATGGTAAGCCC+CGG | 0.611403 | 1.2:+71200488 | None:intergenic |
| AGAGGTACCTAGACTAGGCA+AGG | 0.616371 | 1.2:-71201311 | MS.gene60720:CDS |
| TTGTAATCAGAAATTCCCAA+TGG | 0.619803 | 1.2:+71200746 | None:intergenic |
| CAACAAAGGTGCTCGTGTGT+TGG | 0.620198 | 1.2:-71201071 | MS.gene60720:CDS |
| GTTGTAGAGGTACCTAGACT+AGG | 0.624277 | 1.2:-71201316 | MS.gene60720:CDS |
| CAACAAGGTTGCTTTGCAGG+AGG | 0.634702 | 1.2:-71201130 | MS.gene60720:CDS |
| CCACGGGAGAAGGACTTGAG+TGG | 0.645541 | 1.2:-71200532 | MS.gene60720:CDS |
| ATTGGATGCTAGGCAAGACA+TGG | 0.648366 | 1.2:-71201341 | MS.gene60720:CDS |
| CAATGGCTAAAATGGTTGCA+GGG | 0.655281 | 1.2:+71201696 | None:intergenic |
| ACAATTGCTCCAGATAGTGA+AGG | 0.659707 | 1.2:-71200878 | MS.gene60720:CDS |
| GTGAAAGCTATAAAAGAATG+GGG | 0.667253 | 1.2:-71201280 | MS.gene60720:CDS |
| TCAAGATGGACTGAAGACCA+CGG | 0.668535 | 1.2:-71200549 | MS.gene60720:CDS |
| TGTCCAAGTGAGTATCACTA+GGG | 0.674277 | 1.2:+71201011 | None:intergenic |
| GCCATGTATTCACAAACACT+AGG | 0.680230 | 1.2:+71201370 | None:intergenic |
| GATTTGGCTGAGAACAACAA+AGG | 0.683242 | 1.2:-71201085 | MS.gene60720:CDS |
| GGTACCTAGACTAGGCAAGG+AGG | 0.684618 | 1.2:-71201308 | MS.gene60720:CDS |
| AGTGACACTACGCAAAACAA+CGG | 0.695175 | 1.2:+71200469 | None:intergenic |
| ATGTTAGTCCAGCTTCACGA+AGG | 0.698828 | 1.2:+71200840 | None:intergenic |
| AGGTATATGATGTACCAACA+AGG | 0.704257 | 1.2:-71201145 | MS.gene60720:CDS |
| CTAGAATTGCAGGTCCACCA+GGG | 0.711261 | 1.2:+71200702 | None:intergenic |
| GAGATACATGTACCTAACAG+AGG | 0.713175 | 1.2:-71201410 | MS.gene60720:CDS |
| ACGGGAGAAGGACTTGAGTG+GGG | 0.725736 | 1.2:-71200530 | MS.gene60720:CDS |
| GGATGCTAGGCAAGACATGG+TGG | 0.737305 | 1.2:-71201338 | MS.gene60720:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAGCAAAATATGTTTAAA+TGG | + | chr1.2:71200686-71200705 | None:intergenic | 20.0% |
| !!! | ATATTTTGCTATCTTTACAT+TGG | - | chr1.2:71200693-71200712 | MS.gene60720:CDS | 20.0% |
| ! | AAAATAGGTTTCTCAATTTC+TGG | + | chr1.2:71201283-71201302 | None:intergenic | 25.0% |
| ! | AAAGAACATCAATAAAGCAT+TGG | - | chr1.2:71201420-71201439 | MS.gene60720:CDS | 25.0% |
| ! | AGTGAAAGCTATAAAAGAAT+GGG | - | chr1.2:71200922-71200941 | MS.gene60720:CDS | 25.0% |
| ! | ATTTCTTCATAATTACCTTC+AGG | - | chr1.2:71200749-71200768 | MS.gene60720:CDS | 25.0% |
| ! | GTGATAAATCTATGATCAAA+AGG | - | chr1.2:71200771-71200790 | MS.gene60720:CDS | 25.0% |
| ! | TATCTAAAAAAGTTAGGTAG+AGG | + | chr1.2:71200659-71200678 | None:intergenic | 25.0% |
| !! | ATCATATACCTTTTCACATA+TGG | + | chr1.2:71201049-71201068 | None:intergenic | 25.0% |
| !! | CTGATTACAATTCAATCTTT+TGG | - | chr1.2:71201470-71201489 | MS.gene60720:intron | 25.0% |
| !! | TCATATACCTTTTCACATAT+GGG | + | chr1.2:71201048-71201067 | None:intergenic | 25.0% |
| !!! | GGAAAGTATCTAAAAAAGTT+AGG | + | chr1.2:71200665-71200684 | None:intergenic | 25.0% |
| !!! | GTGATTTTAAAGTAGAAATC+AGG | + | chr1.2:71200574-71200593 | None:intergenic | 25.0% |
| !!! | TTTCATCTTTTCAGGTTTTA+AGG | + | chr1.2:71201548-71201567 | None:intergenic | 25.0% |
| AAAAATTCTTACACATGCGT+TGG | + | chr1.2:71200633-71200652 | None:intergenic | 30.0% | |
| ATCATAGATTTATCACCTGA+AGG | + | chr1.2:71200767-71200786 | None:intergenic | 30.0% | |
| CAGTGAAAGCTATAAAAGAA+TGG | - | chr1.2:71200921-71200940 | MS.gene60720:CDS | 30.0% | |
| GATATTAGTTCAGCAAACTT+GGG | + | chr1.2:71200729-71200748 | None:intergenic | 30.0% | |
| GTGAAAGCTATAAAAGAATG+GGG | - | chr1.2:71200923-71200942 | MS.gene60720:CDS | 30.0% | |
| TGATATTAGTTCAGCAAACT+TGG | + | chr1.2:71200730-71200749 | None:intergenic | 30.0% | |
| TTGTAATCAGAAATTCCCAA+TGG | + | chr1.2:71201460-71201479 | None:intergenic | 30.0% | |
| ! | AAGTGAGTAATCTTTGACTT+TGG | + | chr1.2:71200953-71200972 | None:intergenic | 30.0% |
| !!! | AACCTATTTTTGAGATGGTT+TGG | - | chr1.2:71201293-71201312 | MS.gene60720:CDS | 30.0% |
| !!! | TGAGAAACCTATTTTTGAGA+TGG | - | chr1.2:71201288-71201307 | MS.gene60720:CDS | 30.0% |
| AGGTATATGATGTACCAACA+AGG | - | chr1.2:71201058-71201077 | MS.gene60720:CDS | 35.0% | |
| ATATTCACTAAGCACTTCTC+TGG | + | chr1.2:71201575-71201594 | None:intergenic | 35.0% | |
| CTTTCAAAATCTCCTCTGTT+AGG | + | chr1.2:71200808-71200827 | None:intergenic | 35.0% | |
| GTCCAAACCATCTCAAAAAT+AGG | + | chr1.2:71201298-71201317 | None:intergenic | 35.0% | |
| TACCAACTCACAAAACTCTT+AGG | - | chr1.2:71201016-71201035 | MS.gene60720:CDS | 35.0% | |
| TCCTAGTGTTTGTGAATACA+TGG | - | chr1.2:71200832-71200851 | MS.gene60720:CDS | 35.0% | |
| TTTGTTCAACACAATTGGCT+GGG | + | chr1.2:71200543-71200562 | None:intergenic | 35.0% | |
| ! | TCAACTTGATCTAGAATTGC+AGG | + | chr1.2:71201514-71201533 | None:intergenic | 35.0% |
| ! | TGTGCTTTGTTCAACACAAT+TGG | + | chr1.2:71200548-71200567 | None:intergenic | 35.0% |
| !! | AGAGAAGTGCTTAGTGAATA+TGG | - | chr1.2:71201574-71201593 | MS.gene60720:intron | 35.0% |
| !! | ATGTTCTTTGAAACAATCCC+GGG | + | chr1.2:71201409-71201428 | None:intergenic | 35.0% |
| !! | CATCAATAAAGCATTGGTTG+AGG | - | chr1.2:71201426-71201445 | MS.gene60720:CDS | 35.0% |
| !! | GATGTTCTTTGAAACAATCC+CGG | + | chr1.2:71201410-71201429 | None:intergenic | 35.0% |
| !! | GGTTTCTCAATTTCTGGTAT+TGG | + | chr1.2:71201277-71201296 | None:intergenic | 35.0% |
| !!! | TGTTTTATTTGGCTTTGGAC+CGG | - | chr1.2:71201696-71201715 | MS.gene60720:CDS | 35.0% |
| ACAATTGCTCCAGATAGTGA+AGG | - | chr1.2:71201325-71201344 | MS.gene60720:CDS | 40.0% | |
| ACCTTCTTAAAGATGTTCCC+GGG | - | chr1.2:71201389-71201408 | MS.gene60720:CDS | 40.0% | |
| ACGCAAAACAACGGTTTCAA+TGG | + | chr1.2:71201728-71201747 | None:intergenic | 40.0% | |
| AGAAAGAAGTCAGCTCAAGA+TGG | - | chr1.2:71201640-71201659 | MS.gene60720:CDS | 40.0% | |
| AGTGACACTACGCAAAACAA+CGG | + | chr1.2:71201737-71201756 | None:intergenic | 40.0% | |
| CAAATAGTGCTTGTCCAACA+AGG | + | chr1.2:71201219-71201238 | None:intergenic | 40.0% | |
| CAATGGCTAAAATGGTTGCA+GGG | + | chr1.2:71200510-71200529 | None:intergenic | 40.0% | |
| CACCTTCTTAAAGATGTTCC+CGG | - | chr1.2:71201388-71201407 | MS.gene60720:CDS | 40.0% | |
| CTTGTTGGACAAGCACTATT+TGG | - | chr1.2:71201217-71201236 | MS.gene60720:CDS | 40.0% | |
| CTTTGTTCAACACAATTGGC+TGG | + | chr1.2:71200544-71200563 | None:intergenic | 40.0% | |
| GAGATACATGTACCTAACAG+AGG | - | chr1.2:71200793-71200812 | MS.gene60720:CDS | 40.0% | |
| GATTTGGCTGAGAACAACAA+AGG | - | chr1.2:71201118-71201137 | MS.gene60720:CDS | 40.0% | |
| GCCATGTATTCACAAACACT+AGG | + | chr1.2:71200836-71200855 | None:intergenic | 40.0% | |
| GCTTCGTTTGGCTAAAGATT+TGG | - | chr1.2:71201102-71201121 | MS.gene60720:CDS | 40.0% | |
| TGAATACATGGCACCTTCAT+TGG | - | chr1.2:71200844-71200863 | MS.gene60720:CDS | 40.0% | |
| TGCAGTTCCAATGGCTAAAA+TGG | + | chr1.2:71200518-71200537 | None:intergenic | 40.0% | |
| TGTCCAAGTGAGTATCACTA+GGG | + | chr1.2:71201195-71201214 | None:intergenic | 40.0% | |
| ! | GTTGAGGCTTTTGAACCATT+GGG | - | chr1.2:71201442-71201461 | MS.gene60720:intron | 40.0% |
| !! | CTTGAGTGGGGTGTTTTATT+TGG | - | chr1.2:71201685-71201704 | MS.gene60720:CDS | 40.0% |
| !! | GACCTAAGAGTTTTGTGAGT+TGG | + | chr1.2:71201021-71201040 | None:intergenic | 40.0% |
| !!! | GTTTTATTTGGCTTTGGACC+GGG | - | chr1.2:71201697-71201716 | MS.gene60720:CDS | 40.0% |
| !!! | TGGGGTGTTTTATTTGGCTT+TGG | - | chr1.2:71201691-71201710 | MS.gene60720:CDS | 40.0% |
| !!! | TTTTATTTGGCTTTGGACCG+GGG | - | chr1.2:71201698-71201717 | MS.gene60720:CDS | 40.0% |
| ATGTTAGTCCAGCTTCACGA+AGG | + | chr1.2:71201366-71201385 | None:intergenic | 45.0% | |
| ATTGGATGCTAGGCAAGACA+TGG | - | chr1.2:71200862-71200881 | MS.gene60720:CDS | 45.0% | |
| CCAATGGCTAAAATGGTTGC+AGG | + | chr1.2:71200511-71200530 | None:intergenic | 45.0% | |
| CGAAATTCGCAATGCCCAAA+GGG | - | chr1.2:71200477-71200496 | MS.gene60720:CDS | 45.0% | |
| CTGTCCAAGTGAGTATCACT+AGG | + | chr1.2:71201196-71201215 | None:intergenic | 45.0% | |
| GATAGTGAAGGAGCCATTGA+TGG | - | chr1.2:71201337-71201356 | MS.gene60720:CDS | 45.0% | |
| GGACAAGCACTATTTGGAGA+TGG | - | chr1.2:71201223-71201242 | MS.gene60720:CDS | 45.0% | |
| GTCACTGCAGTTACATTTCG+CGG | - | chr1.2:71201169-71201188 | MS.gene60720:CDS | 45.0% | |
| GTCTTCGCCCATATGTGAAA+AGG | - | chr1.2:71201038-71201057 | MS.gene60720:CDS | 45.0% | |
| TCAATGGCTCCTTCACTATC+TGG | + | chr1.2:71201337-71201356 | None:intergenic | 45.0% | |
| TCTTGCCTAGCATCCAATGA+AGG | + | chr1.2:71200860-71200879 | None:intergenic | 45.0% | |
| TTCACGAAGGTGACCATCAA+TGG | + | chr1.2:71201353-71201372 | None:intergenic | 45.0% | |
| TTCACTAAGCACTTCTCTGG+TGG | + | chr1.2:71201572-71201591 | None:intergenic | 45.0% | |
| TTGATCGTTTGCACCACAAG+TGG | - | chr1.2:71200971-71200990 | MS.gene60720:CDS | 45.0% | |
| ! | ATCTTTTGGATTGCACACCC+TGG | - | chr1.2:71201484-71201503 | MS.gene60720:intron | 45.0% |
| ! | CCTTTGGGCATTGCGAATTT+CGG | + | chr1.2:71200479-71200498 | None:intergenic | 45.0% |
| ! | CTGGTGGCTTTCATCTTTTC+AGG | + | chr1.2:71201556-71201575 | None:intergenic | 45.0% |
| ! | GGTTGAGGCTTTTGAACCAT+TGG | - | chr1.2:71201441-71201460 | MS.gene60720:intron | 45.0% |
| ! | TACCAACAAGGTTGCTTTGC+AGG | - | chr1.2:71201070-71201089 | MS.gene60720:CDS | 45.0% |
| ! | TCCCGGGAACATCTTTAAGA+AGG | + | chr1.2:71201393-71201412 | None:intergenic | 45.0% |
| !! | CCTGCAACCATTTTAGCCAT+TGG | - | chr1.2:71200508-71200527 | MS.gene60720:CDS | 45.0% |
| !! | GTTGTAGAGGTACCTAGACT+AGG | - | chr1.2:71200887-71200906 | MS.gene60720:CDS | 45.0% |
| !! | TCAAGATGGACTGAAGACCA+CGG | - | chr1.2:71201654-71201673 | MS.gene60720:CDS | 45.0% |
| ACAAGTGGTGTAGACATGCC+TGG | - | chr1.2:71200986-71201005 | MS.gene60720:CDS | 50.0% | |
| ACGGTTTCAATGGTAAGCCC+CGG | + | chr1.2:71201718-71201737 | None:intergenic | 50.0% | |
| AGGCATGTCTACACCACTTG+TGG | + | chr1.2:71200987-71201006 | None:intergenic | 50.0% | |
| CCGAAATTCGCAATGCCCAA+AGG | - | chr1.2:71200476-71200495 | MS.gene60720:CDS | 50.0% | |
| CTAGAATTGCAGGTCCACCA+GGG | + | chr1.2:71201504-71201523 | None:intergenic | 50.0% | |
| CTCCTGCAAAGCAACCTTGT+TGG | + | chr1.2:71201075-71201094 | None:intergenic | 50.0% | |
| GCAAGACATGGTGGTTGTAG+AGG | - | chr1.2:71200874-71200893 | MS.gene60720:CDS | 50.0% | |
| TGGCACCTTCATTGGATGCT+AGG | - | chr1.2:71200852-71200871 | MS.gene60720:CDS | 50.0% | |
| ! | ACTCACTTGGACAGCCTTGT+TGG | - | chr1.2:71201202-71201221 | MS.gene60720:CDS | 50.0% |
| ! | CAACAAGGTTGCTTTGCAGG+AGG | - | chr1.2:71201073-71201092 | MS.gene60720:CDS | 50.0% |
| ! | TCTAGAATTGCAGGTCCACC+AGG | + | chr1.2:71201505-71201524 | None:intergenic | 50.0% |
| ! | TTTTGGATTGCACACCCTGG+TGG | - | chr1.2:71201487-71201506 | MS.gene60720:intron | 50.0% |
| !! | AGAGGTACCTAGACTAGGCA+AGG | - | chr1.2:71200892-71200911 | MS.gene60720:CDS | 50.0% |
| !! | CAACAAAGGTGCTCGTGTGT+TGG | - | chr1.2:71201132-71201151 | MS.gene60720:CDS | 50.0% |
| !! | CAAGATGGACTGAAGACCAC+GGG | - | chr1.2:71201655-71201674 | MS.gene60720:CDS | 50.0% |
| !! | GTGAGTTGGTAATCAGCTCC+AGG | + | chr1.2:71201007-71201026 | None:intergenic | 50.0% |
| ACGGGAGAAGGACTTGAGTG+GGG | - | chr1.2:71201673-71201692 | MS.gene60720:CDS | 55.0% | |
| CACGGGAGAAGGACTTGAGT+GGG | - | chr1.2:71201672-71201691 | MS.gene60720:CDS | 55.0% | |
| CGGCCCTAGTGATACTCACT+TGG | - | chr1.2:71201189-71201208 | MS.gene60720:CDS | 55.0% | |
| GATGGTCACCTTCGTGAAGC+TGG | - | chr1.2:71201355-71201374 | MS.gene60720:CDS | 55.0% | |
| GGATGCTAGGCAAGACATGG+TGG | - | chr1.2:71200865-71200884 | MS.gene60720:CDS | 55.0% | |
| GGCTGGGTTTGCAGTTCCAA+TGG | + | chr1.2:71200527-71200546 | None:intergenic | 55.0% | |
| GGTACCTAGACTAGGCAAGG+AGG | - | chr1.2:71200895-71200914 | MS.gene60720:CDS | 55.0% | |
| ! | AGGTTGCTTTGCAGGAGGCA+CGG | - | chr1.2:71201078-71201097 | MS.gene60720:CDS | 55.0% |
| !! | GGAGCTGCTGCACTGATTGT+TGG | - | chr1.2:71201244-71201263 | MS.gene60720:CDS | 55.0% |
| CCACGGGAGAAGGACTTGAG+TGG | - | chr1.2:71201671-71201690 | MS.gene60720:CDS | 60.0% | |
| CCACTCAAGTCCTTCTCCCG+TGG | + | chr1.2:71201674-71201693 | None:intergenic | 60.0% | |
| CGCAATGCCCAAAGGGCAGA+AGG | - | chr1.2:71200484-71200503 | MS.gene60720:CDS | 60.0% | |
| GCAGCCTCCTTGCCTAGTCT+AGG | + | chr1.2:71200902-71200921 | None:intergenic | 60.0% | |
| GGACTGAAGACCACGGGAGA+AGG | - | chr1.2:71201661-71201680 | MS.gene60720:CDS | 60.0% | |
| TGCAGGGCCTTCTGCCCTTT+GGG | + | chr1.2:71200494-71200513 | None:intergenic | 60.0% | |
| TTGCAGGGCCTTCTGCCCTT+TGG | + | chr1.2:71200495-71200514 | None:intergenic | 60.0% | |
| ! | AGGAGGCACGGTGCTTCGTT+TGG | - | chr1.2:71201090-71201109 | MS.gene60720:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 71200463 | 71201762 | 71200463 | ID=MS.gene60720 |
| chr1.2 | mRNA | 71200463 | 71201762 | 71200463 | ID=MS.gene60720.t1;Parent=MS.gene60720 |
| chr1.2 | exon | 71201585 | 71201762 | 71201585 | ID=MS.gene60720.t1.exon1;Parent=MS.gene60720.t1 |
| chr1.2 | CDS | 71201585 | 71201762 | 71201585 | ID=cds.MS.gene60720.t1;Parent=MS.gene60720.t1 |
| chr1.2 | exon | 71200463 | 71201454 | 71200463 | ID=MS.gene60720.t1.exon2;Parent=MS.gene60720.t1 |
| chr1.2 | CDS | 71200463 | 71201454 | 71200463 | ID=cds.MS.gene60720.t1;Parent=MS.gene60720.t1 |
| Gene Sequence |
| Protein sequence |
