AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45607


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45607.t1 MTR_4g097500 98.113 106 2 0 23 128 146 251 4.41e-74 221
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45607.t1 AT5G65840 74.528 106 27 0 23 128 170 275 1.56e-55 174

Find 32 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACACTGGCTGGGTTGAAAA+AGG 0.282027 8.4:+25120218 None:intergenic
TTTGACCTCTACTATGGATT+TGG 0.321070 8.4:-25120246 MS.gene45607:CDS
GGAGGGATGTTTGTGTTTAG+AGG 0.357019 8.4:-25119862 MS.gene45607:CDS
AACTCACAGAAACTTCCTTT+TGG 0.368762 8.4:+25120913 None:intergenic
TAAAGACAATCCATTGGAAA+TGG 0.422792 8.4:+25120385 None:intergenic
AAGGTAAAGCTTACACTGGC+TGG 0.424360 8.4:+25120207 None:intergenic
ATACAGTACAGTTAAGCAAC+AGG 0.447939 8.4:+25119754 None:intergenic
CCGTGCATTACCTTACGGTC+AGG 0.448777 8.4:+25120355 None:intergenic
GTTTGCCTTTGTGAGGGAGG+TGG 0.453478 8.4:-25120936 MS.gene45607:CDS
AGGGTTTGCCTTTGTGAGGG+AGG 0.474618 8.4:-25120939 MS.gene45607:CDS
GAGGGATGTTTGTGTTTAGA+GGG 0.508209 8.4:-25119861 MS.gene45607:CDS
TGTTCAGTTACCATTTCCAA+TGG 0.515281 8.4:-25120395 MS.gene45607:intron
TCTCACCTTCACCATATGGT+GGG 0.516049 8.4:-25120978 None:intergenic
AAAACACCACTTCTATCATC+TGG 0.525139 8.4:+25119994 None:intergenic
CGTGCATTACCTTACGGTCA+GGG 0.525659 8.4:+25120356 None:intergenic
TGGGTGGAGTGTGTAGAGGA+AGG 0.526759 8.4:-25120959 MS.gene45607:CDS
TTGTGAGGGAGGTGGCCAAA+AGG 0.532373 8.4:-25120928 MS.gene45607:CDS
GGGTGGAGTGTGTAGAGGAA+GGG 0.535800 8.4:-25120958 MS.gene45607:CDS
GGAAGGGTTTGCCTTTGTGA+GGG 0.535969 8.4:-25120942 MS.gene45607:CDS
AGGAAGGGTTTGCCTTTGTG+AGG 0.551194 8.4:-25120943 MS.gene45607:CDS
CACCTTCACCATATGGTGGG+TGG 0.557233 8.4:-25120975 None:intergenic
AGGTAAAGCTTACACTGGCT+GGG 0.557305 8.4:+25120208 None:intergenic
TTATGCAGACCCTGACCGTA+AGG 0.565546 8.4:-25120365 MS.gene45607:intron
GGAAAGAGCTATTATATGCA+AGG 0.568553 8.4:-25119840 MS.gene45607:CDS
GCAAGGAAAGACGAAGGCAC+AGG 0.589567 8.4:-25119823 MS.gene45607:CDS
TCTGCATAAAGACAATCCAT+TGG 0.596451 8.4:+25120379 None:intergenic
TTTGGCCACCTCCCTCACAA+AGG 0.623186 8.4:+25120931 None:intergenic
GCCACTCCAGATGATAGAAG+TGG 0.626798 8.4:-25120000 MS.gene45607:CDS
ACCACTTCTATCATCTGGAG+TGG 0.632768 8.4:+25119999 None:intergenic
TTCGGCCAAATCCATAGTAG+AGG 0.635276 8.4:+25120241 None:intergenic
ATGGTGGGTGGAGTGTGTAG+AGG 0.681847 8.4:-25120963 MS.gene45607:CDS
TTATATGCAAGGAAAGACGA+AGG 0.699801 8.4:-25119829 MS.gene45607:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTAAAAAACTTAAAATTAT+TGG + chr8.4:25120021-25120040 None:intergenic 10.0%
!! CTATAATTAAAACTCATAAA+TGG + chr8.4:25119906-25119925 None:intergenic 15.0%
!! TAATTTACGCAATTATTATA+TGG - chr8.4:25120250-25120269 MS.gene45607:CDS 15.0%
!!! AATATTTTACTTTACAACTT+GGG - chr8.4:25120807-25120826 MS.gene45607:intron 15.0%
!!! GTAAAGAATTTTATCAATTT+AGG + chr8.4:25120836-25120855 None:intergenic 15.0%
!!! TAATATTTTACTTTACAACT+TGG - chr8.4:25120806-25120825 MS.gene45607:intron 15.0%
!! TTTAAATTCACTTCATTGAA+GGG - chr8.4:25120121-25120140 MS.gene45607:intron 20.0%
!!! AAAATTCTTTACATTTTGTC+AGG - chr8.4:25120844-25120863 MS.gene45607:intron 20.0%
!!! AAATTCTTTACATTTTGTCA+GGG - chr8.4:25120845-25120864 MS.gene45607:intron 20.0%
!!! TTTTAAATTCACTTCATTGA+AGG - chr8.4:25120120-25120139 MS.gene45607:intron 20.0%
!!! TTTTTGTTTCAAGTTATCTT+GGG - chr8.4:25120053-25120072 MS.gene45607:CDS 20.0%
! AAAATGATATTAACAGGCAA+AGG - chr8.4:25120652-25120671 MS.gene45607:intron 25.0%
! AAACTTAAAATTATTGGCCA+TGG + chr8.4:25120015-25120034 None:intergenic 25.0%
! GACTTCAAAATGATATTAAC+AGG - chr8.4:25120646-25120665 MS.gene45607:intron 25.0%
!! AGTATGGATGATCATTTTAA+TGG - chr8.4:25120178-25120197 MS.gene45607:intron 25.0%
!! CTGATATTTTCTTTTTCTAC+TGG - chr8.4:25120303-25120322 MS.gene45607:intron 25.0%
!! TTTACAAAATGAGTTCTAGT+AGG + chr8.4:25120418-25120437 None:intergenic 25.0%
!!! AATTTTTTTGACCTCTACTA+TGG - chr8.4:25120476-25120495 MS.gene45607:intron 25.0%
!!! GATGATCATTTTAATGGATA+TGG - chr8.4:25120184-25120203 MS.gene45607:intron 25.0%
!!! GTTTTTGTTTCAAGTTATCT+TGG - chr8.4:25120052-25120071 MS.gene45607:CDS 25.0%
AAAATTGTATGCCTGAAAGA+AGG + chr8.4:25120462-25120481 None:intergenic 30.0%
AACATCAAAAACATCGTCTA+AGG + chr8.4:25120942-25120961 None:intergenic 30.0%
ACATCAAAAACATCGTCTAA+GGG + chr8.4:25120941-25120960 None:intergenic 30.0%
ATATTTACCAGCATGCAAAA+TGG + chr8.4:25120619-25120638 None:intergenic 30.0%
ATTTGATGCTTTGCAAAAAG+CGG - chr8.4:25120685-25120704 MS.gene45607:intron 30.0%
CATAAATGGCAAAAACAAAC+AGG + chr8.4:25119892-25119911 None:intergenic 30.0%
TAAAGACAATCCATTGGAAA+TGG + chr8.4:25120346-25120365 None:intergenic 30.0%
! TTGTATTTCAGAAAAGTGCA+AGG + chr8.4:25120543-25120562 None:intergenic 30.0%
AAAAAGAGGTAAGTCGTACA+TGG + chr8.4:25119977-25119996 None:intergenic 35.0%
AAAACACCACTTCTATCATC+TGG + chr8.4:25120737-25120756 None:intergenic 35.0%
AATTAAACCACAAACCGTTG+AGG + chr8.4:25120102-25120121 None:intergenic 35.0%
ATCAAAAACATCGTCTAAGG+GGG + chr8.4:25120939-25120958 None:intergenic 35.0%
CATCAAAAACATCGTCTAAG+GGG + chr8.4:25120940-25120959 None:intergenic 35.0%
GCAGGTTTGATACATTAATC+TGG - chr8.4:25120760-25120779 MS.gene45607:intron 35.0%
GGAAAGAGCTATTATATGCA+AGG - chr8.4:25120888-25120907 MS.gene45607:intron 35.0%
GGTTTGATACATTAATCTGG+TGG - chr8.4:25120763-25120782 MS.gene45607:intron 35.0%
TCAAGTATATTCTGCCTCAA+CGG - chr8.4:25120085-25120104 MS.gene45607:intron 35.0%
TCAAGTTATCTTGGGTTACT+AGG - chr8.4:25120061-25120080 MS.gene45607:CDS 35.0%
TCTGCATAAAGACAATCCAT+TGG + chr8.4:25120352-25120371 None:intergenic 35.0%
TGTTCAGTTACCATTTCCAA+TGG - chr8.4:25120333-25120352 MS.gene45607:intron 35.0%
TTATATGCAAGGAAAGACGA+AGG - chr8.4:25120899-25120918 MS.gene45607:intron 35.0%
TTTGACCTCTACTATGGATT+TGG - chr8.4:25120482-25120501 MS.gene45607:intron 35.0%
TTTGCATGCTGGTAAATATG+TGG - chr8.4:25120620-25120639 MS.gene45607:intron 35.0%
! AACTCACAGAAACTTCCTTT+TGG + chr8.4:25119818-25119837 None:intergenic 35.0%
! ACAATTTCCATTTTGCATGC+TGG - chr8.4:25120609-25120628 MS.gene45607:intron 35.0%
! ATGTACGACTTACCTCTTTT+TGG - chr8.4:25119976-25119995 MS.gene45607:intron 35.0%
! TACCAATTTGGAACAATCAG+TGG - chr8.4:25119859-25119878 MS.gene45607:CDS 35.0%
! TCTTTACATTTTGTCAGGGA+GGG - chr8.4:25120849-25120868 MS.gene45607:intron 35.0%
! TTCTTTACATTTTGTCAGGG+AGG - chr8.4:25120848-25120867 MS.gene45607:intron 35.0%
AACCAATTTCGCCTTCTTTC+AGG - chr8.4:25120448-25120467 MS.gene45607:intron 40.0%
ATCATCCATACTGCTATCCT+TGG + chr8.4:25120170-25120189 None:intergenic 40.0%
ATTTGCCAAGGATAGCAGTA+TGG - chr8.4:25120162-25120181 MS.gene45607:intron 40.0%
GAGGGATGTTTGTGTTTAGA+GGG - chr8.4:25120867-25120886 MS.gene45607:intron 40.0%
GATATCCGTGCATTACCTTA+CGG + chr8.4:25120381-25120400 None:intergenic 40.0%
TCTGTGTTGCAAATTTGCCA+AGG - chr8.4:25120150-25120169 MS.gene45607:intron 40.0%
TTCCTACCTTCACCAAAAAG+AGG + chr8.4:25119991-25120010 None:intergenic 40.0%
! AGCCACTGATTGTTCCAAAT+TGG + chr8.4:25119864-25119883 None:intergenic 40.0%
! CAGGGTAGGTTTTACCAATT+TGG - chr8.4:25119847-25119866 MS.gene45607:CDS 40.0%
!! GACTTACCTCTTTTTGGTGA+AGG - chr8.4:25119982-25120001 MS.gene45607:intron 40.0%
!! TACCTCTTTTTGGTGAAGGT+AGG - chr8.4:25119986-25120005 MS.gene45607:intron 40.0%
AAGGTAAAGCTTACACTGGC+TGG + chr8.4:25120524-25120543 None:intergenic 45.0%
ACCACTTCTATCATCTGGAG+TGG + chr8.4:25120732-25120751 None:intergenic 45.0%
GGAGGGATGTTTGTGTTTAG+AGG - chr8.4:25120866-25120885 MS.gene45607:intron 45.0%
GTGCAAGGTAAAGCTTACAC+TGG + chr8.4:25120528-25120547 None:intergenic 45.0%
TATTCTGCCTCAACGGTTTG+TGG - chr8.4:25120092-25120111 MS.gene45607:intron 45.0%
TGCCTGAAAGAAGGCGAAAT+TGG + chr8.4:25120453-25120472 None:intergenic 45.0%
TTCGGCCAAATCCATAGTAG+AGG + chr8.4:25120490-25120509 None:intergenic 45.0%
TTGGTGAAGGTAGGAATCCA+TGG - chr8.4:25119995-25120014 MS.gene45607:CDS 45.0%
! AGGTAAAGCTTACACTGGCT+GGG + chr8.4:25120523-25120542 None:intergenic 45.0%
! CTGTGAGTTGAAGAGATGCA+GGG - chr8.4:25119829-25119848 MS.gene45607:CDS 45.0%
! TACACTGGCTGGGTTGAAAA+AGG + chr8.4:25120513-25120532 None:intergenic 45.0%
! TAGAAGTGGTGTTTTGCAGC+AGG - chr8.4:25120742-25120761 MS.gene45607:intron 45.0%
! TCTGTGAGTTGAAGAGATGC+AGG - chr8.4:25119828-25119847 MS.gene45607:CDS 45.0%
! TGGCTGGGTTGAAAAAGGTT+CGG + chr8.4:25120508-25120527 None:intergenic 45.0%
AGGAAGGGTTTGCCTTTGTG+AGG - chr8.4:25119785-25119804 MS.gene45607:CDS 50.0%
CCTGACCGTAAGGTAATGCA+CGG - chr8.4:25120373-25120392 MS.gene45607:CDS 50.0%
CGTGCATTACCTTACGGTCA+GGG + chr8.4:25120375-25120394 None:intergenic 50.0%
GAGTTGAAGAGATGCAGGGT+AGG - chr8.4:25119833-25119852 MS.gene45607:CDS 50.0%
GCCACTCCAGATGATAGAAG+TGG - chr8.4:25120728-25120747 MS.gene45607:intron 50.0%
GGAAGGGTTTGCCTTTGTGA+GGG - chr8.4:25119786-25119805 MS.gene45607:CDS 50.0%
TTATGCAGACCCTGACCGTA+AGG - chr8.4:25120363-25120382 MS.gene45607:intron 50.0%
AGGGTTTGCCTTTGTGAGGG+AGG - chr8.4:25119789-25119808 MS.gene45607:CDS 55.0%
CCGTGCATTACCTTACGGTC+AGG + chr8.4:25120376-25120395 None:intergenic 55.0%
GGGTGGAGTGTGTAGAGGAA+GGG - chr8.4:25119770-25119789 MS.gene45607:CDS 55.0%
GTTTGCCTTTGTGAGGGAGG+TGG - chr8.4:25119792-25119811 MS.gene45607:CDS 55.0%
TGGGTGGAGTGTGTAGAGGA+AGG - chr8.4:25119769-25119788 MS.gene45607:CDS 55.0%
TTGTGAGGGAGGTGGCCAAA+AGG - chr8.4:25119800-25119819 MS.gene45607:CDS 55.0%
TTTGGCCACCTCCCTCACAA+AGG + chr8.4:25119800-25119819 None:intergenic 55.0%
! GCAAGGAAAGACGAAGGCAC+AGG - chr8.4:25120905-25120924 MS.gene45607:intron 55.0%
!! ATGGTGGGTGGAGTGTGTAG+AGG - chr8.4:25119765-25119784 MS.gene45607:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 25119765 25120985 25119765 ID=MS.gene45607
chr8.4 mRNA 25119765 25120985 25119765 ID=MS.gene45607.t1;Parent=MS.gene45607
chr8.4 exon 25120920 25120985 25120920 ID=MS.gene45607.t1.exon1;Parent=MS.gene45607.t1
chr8.4 CDS 25120920 25120985 25120920 ID=cds.MS.gene45607.t1;Parent=MS.gene45607.t1
chr8.4 exon 25120366 25120410 25120366 ID=MS.gene45607.t1.exon2;Parent=MS.gene45607.t1
chr8.4 CDS 25120366 25120410 25120366 ID=cds.MS.gene45607.t1;Parent=MS.gene45607.t1
chr8.4 exon 25120221 25120280 25120221 ID=MS.gene45607.t1.exon3;Parent=MS.gene45607.t1
chr8.4 CDS 25120221 25120280 25120221 ID=cds.MS.gene45607.t1;Parent=MS.gene45607.t1
chr8.4 exon 25119987 25120082 25119987 ID=MS.gene45607.t1.exon4;Parent=MS.gene45607.t1
chr8.4 CDS 25119987 25120082 25119987 ID=cds.MS.gene45607.t1;Parent=MS.gene45607.t1
chr8.4 exon 25119765 25119884 25119765 ID=MS.gene45607.t1.exon5;Parent=MS.gene45607.t1
chr8.4 CDS 25119765 25119884 25119765 ID=cds.MS.gene45607.t1;Parent=MS.gene45607.t1
Gene Sequence

>MS.gene45607

ATGGTGGGTGGAGTGTGTAGAGGAAGGGTTTGCCTTTGTGAGGGAGGTGGCCAAAAGGAAGTTTCTGTGAGTTGAAGAGATGCAGGGTAGGTTTTACCAATTTGGAACAATCAGTGGCTTGACACCTGTTTGTTTTTGCCATTTATGAGTTTTAATTATAGATTTCAGAATAGAATTAGAAAAAAGATAGAGTTAGAGATACATTTATACCATGTACGACTTACCTCTTTTTGGTGAAGGTAGGAATCCATGGCCAATAATTTTAAGTTTTTTAGATCAATTGTTATGTTTTTGTTTCAAGTTATCTTGGGTTACTAGGCTCAAGTATATTCTGCCTCAACGGTTTGTGGTTTAATTTTAAATTCACTTCATTGAAGGGTCACTATCTGTGTTGCAAATTTGCCAAGGATAGCAGTATGGATGATCATTTTAATGGATATGGTTCTCTATACTTTAGCATATCTGAATTTTGAAGAATATTCATCTAATTTACGCAATTATTATATGGTATGAACATGTACTTGACGTCAAGACTCAACTGATATTTTCTTTTTCTACTGGTATTGTATGTTCAGTTACCATTTCCAATGGATTGTCTTTATGCAGACCCTGACCGTAAGGTAATGCACGGATATCATGTTGAACATTTACCTACTAGAACTCATTTTGTAAATGTCTATTTTAACCAATTTCGCCTTCTTTCAGGCATACAATTTTTTTGACCTCTACTATGGATTTGGCCGAACCTTTTTCAACCCAGCCAGTGTAAGCTTTACCTTGCACTTTTCTGAAATACAAATATGTGCGCGCGCACGCGCATACATACATATATATACATTGTTAGACAATTTCCATTTTGCATGCTGGTAAATATGTGGTTTGACTTCAAAATGATATTAACAGGCAAAGGTGTTATCGAGATTTGATGCTTTGCAAAAAGCGGTCAAGAACTATACAATAGAAGCCACTCCAGATGATAGAAGTGGTGTTTTGCAGCAGGTTTGATACATTAATCTGGTGGCAAAAGAATGAAAACAAACTTAATATTTTACTTTACAACTTGGGTTTCCTAAATTGATAAAATTCTTTACATTTTGTCAGGGAGGGATGTTTGTGTTTAGAGGGAAAGAGCTATTATATGCAAGGAAAGACGAAGGCACAGGTGATCATGCCCCCTTAGACGATGTTTTTGATGTTTGCTGCAAAGTGCCTGTTGCTTAA

Protein sequence

>MS.gene45607.t1

MVGGVCRGRVCLCEGGGQKEVSLPFPMDCLYADPDRKAYNFFDLYYGFGRTFFNPASAKVLSRFDALQKAVKNYTIEATPDDRSGVLQQGGMFVFRGKELLYARKDEGTGDHAPLDDVFDVCCKVPVA