AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08658


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08658.t1 MTR_8g086020 96.875 160 5 0 1 160 1 160 1.85e-114 321
MS.gene08658.t1 MTR_4g070860 52.448 143 62 3 15 155 14 152 9.57e-44 142
MS.gene08658.t1 MTR_1g022495 48.795 166 73 6 1 160 1 160 2.25e-41 136
MS.gene08658.t1 MTR_3g117120 51.064 141 64 2 15 155 14 149 1.02e-37 126
MS.gene08658.t1 MTR_3g112220 57.692 78 33 0 33 110 2 79 4.07e-25 92.8
MS.gene08658.t1 MTR_1g087040 40.141 142 74 5 1 140 1 133 8.61e-22 85.9
MS.gene08658.t1 MTR_8g015250 39.000 100 59 1 4 103 45 142 2.41e-15 70.1
MS.gene08658.t1 MTR_1g017570 40.426 94 53 1 26 119 51 141 5.12e-14 65.5
MS.gene08658.t1 MTR_7g029400 39.080 87 53 0 17 103 66 152 5.40e-14 66.6
MS.gene08658.t1 MTR_5g015090 38.393 112 59 4 2 110 32 136 7.52e-14 65.5
MS.gene08658.t1 MTR_4g097440 33.621 116 74 1 2 114 39 154 1.41e-13 65.1
MS.gene08658.t1 MTR_3g109310 31.132 106 73 0 9 114 100 205 1.88e-11 60.8
MS.gene08658.t1 MTR_6g016375 34.000 100 66 0 4 103 59 158 2.95e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08658.t1 AT1G75390 51.899 158 68 4 2 154 10 164 5.72e-46 148
MS.gene08658.t1 AT4G34590 50.725 138 59 3 31 160 23 159 2.33e-37 126
MS.gene08658.t1 AT2G18160 42.405 158 68 4 21 157 15 170 2.10e-32 114
MS.gene08658.t1 AT1G75390 54.082 98 43 1 2 99 10 105 6.61e-30 105
MS.gene08658.t1 AT3G62420 38.621 145 85 3 11 155 2 142 1.11e-27 100
MS.gene08658.t1 AT4G37730 38.235 102 52 3 13 103 171 272 1.23e-13 67.0
MS.gene08658.t1 AT3G30530 39.216 102 61 1 3 103 48 149 3.97e-12 61.2
MS.gene08658.t1 AT5G49450 45.122 82 45 0 33 114 14 95 4.04e-12 60.8
MS.gene08658.t1 AT1G13600 35.455 110 66 2 17 126 70 174 4.32e-12 61.6
MS.gene08658.t1 AT5G15830 34.426 122 77 1 13 131 52 173 6.77e-12 60.8
MS.gene08658.t1 AT2G04038 37.931 87 53 1 17 103 57 142 3.04e-11 58.9

Find 27 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTGAAAATGCTATTCTT+AGG 0.244925 4.3:-14743347 MS.gene08658:CDS
ATGATTGGTTGTTGATTAAC+AGG 0.261835 4.3:+14743186 None:intergenic
TTGATCTTCGTGCTGATTCT+CGG 0.312597 4.3:+14743487 None:intergenic
GTTCAATGATTGTGGATTCT+TGG 0.317045 4.3:-14743226 MS.gene08658:CDS
TGCTATTCTTAGGGTTCAAA+TGG 0.377563 4.3:-14743337 MS.gene08658:CDS
CCTGGTGGAACTTATTCATC+TGG 0.409672 4.3:-14743606 MS.gene08658:CDS
ATCTGAAAATGCTATTCTTA+GGG 0.417867 4.3:-14743346 MS.gene08658:CDS
GATGAAGAAACAGCAACATA+TGG 0.436530 4.3:-14743463 MS.gene08658:CDS
GATCAATTGTTCAATGATTG+TGG 0.455410 4.3:-14743234 MS.gene08658:CDS
TCTTCGTGCTGATTCTCGGT+TGG 0.456704 4.3:+14743491 None:intergenic
TGATCAGAAGAAGCCATGAT+TGG 0.513873 4.3:+14743171 None:intergenic
TGTTAATCAACAACCAATCA+TGG 0.519818 4.3:-14743184 MS.gene08658:CDS
GAGAATCAGCACGAAGATCA+AGG 0.528035 4.3:-14743485 MS.gene08658:CDS
GACCTTGATATGGCTTCTCC+TGG 0.533330 4.3:-14743624 None:intergenic
CTTCAAAACTCTGGATCAGA+GGG 0.550324 4.3:-14743573 MS.gene08658:CDS
ACAAGCTCTCTTCAAAACTC+TGG 0.550443 4.3:-14743582 MS.gene08658:CDS
CTTGATATGGCTTCTCCTGG+TGG 0.579058 4.3:-14743621 None:intergenic
TCTTCAAAACTCTGGATCAG+AGG 0.579120 4.3:-14743574 MS.gene08658:CDS
GGGAGATCATAATAACATGC+AGG 0.579180 4.3:-14743553 MS.gene08658:CDS
AAATGGCTGAACTTAGCCAC+AGG 0.585823 4.3:-14743320 MS.gene08658:CDS
CCAGATGAATAAGTTCCACC+AGG 0.607477 4.3:+14743606 None:intergenic
ATTGTGGATTCTTGGACACA+TGG 0.610654 4.3:-14743218 MS.gene08658:CDS
ACATAACAGATCAAAAGAAG+AGG 0.628528 4.3:-14743527 MS.gene08658:CDS
ATTCAAATAAGCGCAAACGT+TGG 0.641513 4.3:-14743399 MS.gene08658:CDS
CATTAAGTGATTGCAACCTG+TGG 0.653582 4.3:+14743304 None:intergenic
TACTCAGATGTACCTGAACG+TGG 0.654069 4.3:-14743370 MS.gene08658:CDS
TGATTGGTTGTTGATTAACA+GGG 0.670028 4.3:+14743187 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATCTGAAAATGCTATTCTT+AGG - chr4.3:14743423-14743442 MS.gene08658:CDS 25.0%
! ATCTGAAAATGCTATTCTTA+GGG - chr4.3:14743424-14743443 MS.gene08658:CDS 25.0%
ACATAACAGATCAAAAGAAG+AGG - chr4.3:14743243-14743262 MS.gene08658:CDS 30.0%
GATCAATTGTTCAATGATTG+TGG - chr4.3:14743536-14743555 MS.gene08658:CDS 30.0%
TGTTAATCAACAACCAATCA+TGG - chr4.3:14743586-14743605 MS.gene08658:CDS 30.0%
! ATGATTGGTTGTTGATTAAC+AGG + chr4.3:14743587-14743606 None:intergenic 30.0%
! TGATTGGTTGTTGATTAACA+GGG + chr4.3:14743586-14743605 None:intergenic 30.0%
!!! ATCAAGTAACTCACTTTTTC+AGG - chr4.3:14743508-14743527 MS.gene08658:CDS 30.0%
!!! CTTTTTTCAGCTGTTCAATT+TGG + chr4.3:14743346-14743365 None:intergenic 30.0%
ATTCAAATAAGCGCAAACGT+TGG - chr4.3:14743371-14743390 MS.gene08658:CDS 35.0%
GATGAAGAAACAGCAACATA+TGG - chr4.3:14743307-14743326 MS.gene08658:CDS 35.0%
GTTCAATGATTGTGGATTCT+TGG - chr4.3:14743544-14743563 MS.gene08658:CDS 35.0%
TGCTATTCTTAGGGTTCAAA+TGG - chr4.3:14743433-14743452 MS.gene08658:CDS 35.0%
ACAAGCTCTCTTCAAAACTC+TGG - chr4.3:14743188-14743207 MS.gene08658:CDS 40.0%
ATTGTGGATTCTTGGACACA+TGG - chr4.3:14743552-14743571 MS.gene08658:CDS 40.0%
CATTAAGTGATTGCAACCTG+TGG + chr4.3:14743469-14743488 None:intergenic 40.0%
CTTCAAAACTCTGGATCAGA+GGG - chr4.3:14743197-14743216 MS.gene08658:CDS 40.0%
GGGAGATCATAATAACATGC+AGG - chr4.3:14743217-14743236 MS.gene08658:CDS 40.0%
TCTTCAAAACTCTGGATCAG+AGG - chr4.3:14743196-14743215 MS.gene08658:CDS 40.0%
TGATCAGAAGAAGCCATGAT+TGG + chr4.3:14743602-14743621 None:intergenic 40.0%
! CATTTTCAGATTCCACGTTC+AGG + chr4.3:14743415-14743434 None:intergenic 40.0%
!! TTGATCTTCGTGCTGATTCT+CGG + chr4.3:14743286-14743305 None:intergenic 40.0%
AAATGGCTGAACTTAGCCAC+AGG - chr4.3:14743450-14743469 MS.gene08658:CDS 45.0%
CCAGATGAATAAGTTCCACC+AGG + chr4.3:14743167-14743186 None:intergenic 45.0%
CCTGGTGGAACTTATTCATC+TGG - chr4.3:14743164-14743183 MS.gene08658:CDS 45.0%
GAGAATCAGCACGAAGATCA+AGG - chr4.3:14743285-14743304 MS.gene08658:CDS 45.0%
TACTCAGATGTACCTGAACG+TGG - chr4.3:14743400-14743419 MS.gene08658:CDS 45.0%
!! TCTTCGTGCTGATTCTCGGT+TGG + chr4.3:14743282-14743301 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 14743155 14743637 14743155 ID=MS.gene08658
chr4.3 mRNA 14743155 14743637 14743155 ID=MS.gene08658.t1;Parent=MS.gene08658
chr4.3 exon 14743155 14743637 14743155 ID=MS.gene08658.t1.exon1;Parent=MS.gene08658.t1
chr4.3 CDS 14743155 14743637 14743155 ID=cds.MS.gene08658.t1;Parent=MS.gene08658.t1
Gene Sequence

>MS.gene08658

ATGGCTTCTCCTGGTGGAACTTATTCATCTGGCACAAGCTCTCTTCAAAACTCTGGATCAGAGGGAGATCATAATAACATGCAGGTGAACATAACAGATCAAAAGAAGAGGAAGAGAATGCAATCCAACCGAGAATCAGCACGAAGATCAAGGATGAAGAAACAGCAACATATGGAAGATCTAAGCAACCAAATTGAACAGCTGAAAAAAGAGAACATTCAAATAAGCGCAAACGTTGGAGTAACTACTCAGATGTACCTGAACGTGGAATCTGAAAATGCTATTCTTAGGGTTCAAATGGCTGAACTTAGCCACAGGTTGCAATCACTTAATGATATTATTCATTACATTGAATCAAGTAACTCACTTTTTCAGGAGACAGATCAATTGTTCAATGATTGTGGATTCTTGGACACATGGAACACTTTCCCTGTTAATCAACAACCAATCATGGCTTCTTCTGATCATATGTTAATGTATTGA

Protein sequence

>MS.gene08658.t1

MASPGGTYSSGTSSLQNSGSEGDHNNMQVNITDQKKRKRMQSNRESARRSRMKKQQHMEDLSNQIEQLKKENIQISANVGVTTQMYLNVESENAILRVQMAELSHRLQSLNDIIHYIESSNSLFQETDQLFNDCGFLDTWNTFPVNQQPIMASSDHMLMY