AlfalfaGEDB | MS.gene59101

M. sativa cultivar XinJiangDaYe / MS.gene59101

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59101.t1	XP_003589664.1	76.2	143	28	1	1	137	1	143	1.30E-48	202.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59101.t1	G7IA04	76.2	143	28	1	1	137	1	143	9.2e-49	202.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049137	MS.gene59101	0.833501	5.14E-56	-1.69E-46
MS.gene049171	MS.gene59101	0.856221	3.72E-62	-1.69E-46
MS.gene049222	MS.gene59101	0.80065	1.30E-48	-1.69E-46
MS.gene049257	MS.gene59101	0.805219	1.48E-49	-1.69E-46
MS.gene049285	MS.gene59101	0.813087	3.06E-51	-1.69E-46
MS.gene050163	MS.gene59101	0.803013	4.27E-49	-1.69E-46
MS.gene050315	MS.gene59101	0.80502	1.63E-49	-1.69E-46
MS.gene050479	MS.gene59101	-0.801765	7.72E-49	-1.69E-46
MS.gene050507	MS.gene59101	0.804956	1.68E-49	-1.69E-46
MS.gene050577	MS.gene59101	0.832261	1.05E-55	-1.69E-46
MS.gene050699	MS.gene59101	0.831424	1.68E-55	-1.69E-46
MS.gene050747	MS.gene59101	0.810249	1.27E-50	-1.69E-46
MS.gene050781	MS.gene59101	0.824816	6.57E-54	-1.69E-46
MS.gene05081	MS.gene59101	0.82044	6.85E-53	-1.69E-46
MS.gene050950	MS.gene59101	0.810936	9.00E-51	-1.69E-46
MS.gene05100	MS.gene59101	0.800146	1.65E-48	-1.69E-46
MS.gene051171	MS.gene59101	0.800622	1.32E-48	-1.69E-46
MS.gene051244	MS.gene59101	0.833894	4.10E-56	-1.69E-46
MS.gene051666	MS.gene59101	0.809753	1.62E-50	-1.69E-46
MS.gene051838	MS.gene59101	0.810286	1.24E-50	-1.69E-46
MS.gene051973	MS.gene59101	-0.827616	1.42E-54	-1.69E-46
MS.gene052015	MS.gene59101	-0.800137	1.66E-48	-1.69E-46
MS.gene052501	MS.gene59101	0.829815	4.17E-55	-1.69E-46
MS.gene052503	MS.gene59101	0.823231	1.55E-53	-1.69E-46
MS.gene052505	MS.gene59101	0.819855	9.33E-53	-1.69E-46
MS.gene052606	MS.gene59101	-0.803006	4.28E-49	-1.69E-46
MS.gene052615	MS.gene59101	0.814698	1.35E-51	-1.69E-46
MS.gene052823	MS.gene59101	0.824034	1.00E-53	-1.69E-46
MS.gene052825	MS.gene59101	0.828303	9.69E-55	-1.69E-46
MS.gene053203	MS.gene59101	0.808051	3.74E-50	-1.69E-46
MS.gene053330	MS.gene59101	0.827069	1.92E-54	-1.69E-46
MS.gene053480	MS.gene59101	0.809301	2.02E-50	-1.69E-46
MS.gene053579	MS.gene59101	0.819914	9.04E-53	-1.69E-46
MS.gene053824	MS.gene59101	0.807983	3.87E-50	-1.69E-46
MS.gene054107	MS.gene59101	0.801318	9.53E-49	-1.69E-46
MS.gene054343	MS.gene59101	0.862687	4.25E-64	-1.69E-46
MS.gene054344	MS.gene59101	0.825151	5.48E-54	-1.69E-46
MS.gene054408	MS.gene59101	0.808937	2.42E-50	-1.69E-46
MS.gene05470	MS.gene59101	0.863246	2.86E-64	-1.69E-46
MS.gene054884	MS.gene59101	-0.841588	4.34E-58	-1.69E-46
MS.gene055206	MS.gene59101	0.800779	1.23E-48	-1.69E-46
MS.gene055362	MS.gene59101	0.809879	1.52E-50	-1.69E-46
MS.gene055370	MS.gene59101	0.866003	3.92E-65	-1.69E-46
MS.gene055502	MS.gene59101	0.813511	2.47E-51	-1.69E-46
MS.gene055577	MS.gene59101	0.811854	5.68E-51	-1.69E-46
MS.gene055748	MS.gene59101	0.806912	6.53E-50	-1.69E-46
MS.gene05589	MS.gene59101	0.801814	7.54E-49	-1.69E-46
MS.gene056351	MS.gene59101	0.823283	1.51E-53	-1.69E-46
MS.gene056633	MS.gene59101	0.871071	9.04E-67	-1.69E-46
MS.gene056664	MS.gene59101	0.810019	1.42E-50	-1.69E-46
MS.gene056692	MS.gene59101	0.807206	5.66E-50	-1.69E-46
MS.gene056877	MS.gene59101	0.817965	2.51E-52	-1.69E-46
MS.gene056988	MS.gene59101	-0.821431	4.05E-53	-1.69E-46
MS.gene057048	MS.gene59101	0.834184	3.47E-56	-1.69E-46
MS.gene057196	MS.gene59101	0.895123	1.29E-75	-1.69E-46
MS.gene057332	MS.gene59101	0.85703	2.15E-62	-1.69E-46
MS.gene057658	MS.gene59101	0.885408	8.35E-72	-1.69E-46
MS.gene057831	MS.gene59101	0.827529	1.49E-54	-1.69E-46
MS.gene058076	MS.gene59101	-0.804638	1.96E-49	-1.69E-46
MS.gene05837	MS.gene59101	0.801934	7.12E-49	-1.69E-46
MS.gene058418	MS.gene59101	-0.815828	7.57E-52	-1.69E-46
MS.gene058419	MS.gene59101	0.833768	4.41E-56	-1.69E-46
MS.gene058444	MS.gene59101	0.802854	4.60E-49	-1.69E-46
MS.gene058544	MS.gene59101	0.863532	2.33E-64	-1.69E-46
MS.gene058746	MS.gene59101	0.857344	1.74E-62	-1.69E-46
MS.gene058838	MS.gene59101	0.855207	7.36E-62	-1.69E-46
MS.gene059022	MS.gene59101	0.800577	1.35E-48	-1.69E-46
MS.gene059023	MS.gene59101	0.862788	3.95E-64	-1.69E-46
MS.gene059103	MS.gene59101	-0.802662	5.04E-49	-1.69E-46
MS.gene05914	MS.gene59101	0.890088	1.35E-73	-1.69E-46
MS.gene05934	MS.gene59101	0.806051	9.93E-50	-1.69E-46
MS.gene059480	MS.gene59101	-0.801591	8.38E-49	-1.69E-46
MS.gene059512	MS.gene59101	0.844103	9.30E-59	-1.69E-46
MS.gene059677	MS.gene59101	0.837396	5.33E-57	-1.69E-46
MS.gene059718	MS.gene59101	0.806575	7.70E-50	-1.69E-46
MS.gene059756	MS.gene59101	0.839473	1.55E-57	-1.69E-46
MS.gene060087	MS.gene59101	-0.805032	1.62E-49	-1.69E-46
MS.gene060762	MS.gene59101	0.804345	2.26E-49	-1.69E-46
MS.gene06077	MS.gene59101	0.825388	4.82E-54	-1.69E-46
MS.gene06092	MS.gene59101	0.85067	1.46E-60	-1.69E-46
MS.gene061150	MS.gene59101	0.845266	4.52E-59	-1.69E-46
MS.gene061231	MS.gene59101	0.822904	1.85E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59101.t1	MTR_1g031740	84.615	143	16	1	1	137	1	143	1.85e-78	230

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 43 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGAGGAGAATCTGGGTTT+TGG	0.221469	1.4:-16166829	None:intergenic
GGAGAGTGGCTGGGTGAAAA+TGG	0.232098	1.4:+16167277	MS.gene59101:CDS
GAAGAAGAAGGAGGAGAATC+TGG	0.270359	1.4:-16166836	None:intergenic
TGGAGGAAGATTGGTTTGTT+TGG	0.288556	1.4:-16166861	None:intergenic
GGTCGACGGGGAAGCTTGTT+TGG	0.309086	1.4:-16166932	None:intergenic
GGAGGAAGATTGGTTTGTTT+GGG	0.355312	1.4:-16166860	None:intergenic
GACCACGATGGATTTGTTCT+TGG	0.365556	1.4:+16167210	MS.gene59101:CDS
TAATGAAGTAGTGGAGTTCT+TGG	0.377298	1.4:-16166906	None:intergenic
ACCACGATGGATTTGTTCTT+GGG	0.379690	1.4:+16167211	MS.gene59101:CDS
GAGAGTGGCTGGGTGAAAAT+GGG	0.391269	1.4:+16167278	MS.gene59101:CDS
GGTGTTAGTGGAGCGAAATC+TGG	0.391808	1.4:-16167305	None:intergenic
ATTAGTAATAAGAATGGGTT+TGG	0.396058	1.4:-16166795	None:intergenic
TTGCTACTTACTGGTGTTAG+TGG	0.404594	1.4:-16167317	None:intergenic
CGAAGATGGTGATGGTCGAC+GGG	0.447807	1.4:-16166945	None:intergenic
GAGGGAAACAGGAGAGTGGC+TGG	0.452230	1.4:+16167267	MS.gene59101:CDS
TCAATTTGCATGAGCGAAGA+TGG	0.459616	1.4:-16166959	None:intergenic
AAGAAGAAGGAGGAGAATCT+GGG	0.467707	1.4:-16166835	None:intergenic
GTTGCATTAGTAATAAGAAT+GGG	0.473568	1.4:-16166800	None:intergenic
GTTTGTTTGGGTGAAGAAGA+AGG	0.476332	1.4:-16166848	None:intergenic
AGTTGCATTAGTAATAAGAA+TGG	0.478851	1.4:-16166801	None:intergenic
TTCTTGGGACAAGTATTTGA+AGG	0.485835	1.4:+16167226	MS.gene59101:CDS
ATTGAACGTGCCCTTGGCGC+TGG	0.500974	1.4:+16166977	MS.gene59101:CDS
ATGCAAATTGAACGTGCCCT+TGG	0.511985	1.4:+16166971	MS.gene59101:CDS
GACAGAGCATACTTGAGGTC+AGG	0.519678	1.4:-16167019	None:intergenic
TGTTATAGGTGATTATGAGT+GGG	0.521671	1.4:+16167402	MS.gene59101:intron
CTCTGAAGCTACCAGCGCCA+AGG	0.529782	1.4:-16166988	None:intergenic
GTTATAGGTGATTATGAGTG+GGG	0.532217	1.4:+16167403	MS.gene59101:intron
GCGAAGATGGTGATGGTCGA+CGG	0.545160	1.4:-16166946	None:intergenic
TCTTGGGACAAGTATTTGAA+GGG	0.567555	1.4:+16167227	MS.gene59101:CDS
GAGTTCTTGGATCTTAGTGA+AGG	0.568920	1.4:-16166893	None:intergenic
CGACGGGGAAGCTTGTTTGG+AGG	0.576924	1.4:-16166929	None:intergenic
AGAGTGGCTGGGTGAAAATG+GGG	0.590303	1.4:+16167279	MS.gene59101:CDS
TCTGAAGCTACCAGCGCCAA+GGG	0.590855	1.4:-16166987	None:intergenic
TTGTTATAGGTGATTATGAG+TGG	0.595225	1.4:+16167401	MS.gene59101:intron
GTTTGGAGGTAATGAAGTAG+TGG	0.605102	1.4:-16166915	None:intergenic
TGTTTGGGTGAAGAAGAAGG+AGG	0.610626	1.4:-16166845	None:intergenic
AGGGAAACAGGAGAGTGGCT+GGG	0.620803	1.4:+16167268	MS.gene59101:CDS
GGCGCTGGTAGCTTCAGAGA+CGG	0.635842	1.4:+16166992	MS.gene59101:CDS
AGCTGAGGGAAACAGGAGAG+TGG	0.671817	1.4:+16167263	MS.gene59101:CDS
GAAGATGGTGATGGTCGACG+GGG	0.719959	1.4:-16166944	None:intergenic
TGCATGAGCGAAGATGGTGA+TGG	0.732147	1.4:-16166953	None:intergenic
TCCCAAGAACAAATCCATCG+TGG	0.736351	1.4:-16167212	None:intergenic
AGACGTAAAGAAGACCACGA+TGG	0.745068	1.4:+16167198	MS.gene59101:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTATAACATTATGATGAAT+AGG	+	chr1.4:16167072-16167091	MS.gene59101:intron	15.0%
!!!	CACTTTTAACATTATGAAAT+TGG	-	chr1.4:16167114-16167133	None:intergenic	20.0%
!	AGTTGCATTAGTAATAAGAA+TGG	-	chr1.4:16166804-16166823	None:intergenic	25.0%
!	ATCAAAATCCAGAATGAAAT+TGG	-	chr1.4:16167139-16167158	None:intergenic	25.0%
!	GTTGCATTAGTAATAAGAAT+GGG	-	chr1.4:16166803-16166822	None:intergenic	25.0%
!	TGTTAAAGAGGCAAAAAATA+CGG	-	chr1.4:16167358-16167377	None:intergenic	25.0%
!!	ATTAGTAATAAGAATGGGTT+TGG	-	chr1.4:16166798-16166817	None:intergenic	25.0%
!!!	TATTTTTTGCCTCTTTAACA+AGG	+	chr1.4:16167358-16167377	MS.gene59101:intron	25.0%
	TGTTATAGGTGATTATGAGT+GGG	+	chr1.4:16167402-16167421	MS.gene59101:intron	30.0%
	TTGTTATAGGTGATTATGAG+TGG	+	chr1.4:16167401-16167420	MS.gene59101:intron	30.0%
!!	CATGTCTTTTGCTTTGTTAT+AGG	+	chr1.4:16167388-16167407	MS.gene59101:intron	30.0%
	GTTATAGGTGATTATGAGTG+GGG	+	chr1.4:16167403-16167422	MS.gene59101:intron	35.0%
	TCTTGGGACAAGTATTTGAA+GGG	+	chr1.4:16167227-16167246	MS.gene59101:CDS	35.0%
	TGCTACAATCCTTGTTAAAG+AGG	-	chr1.4:16167370-16167389	None:intergenic	35.0%
	TTCTTGGGACAAGTATTTGA+AGG	+	chr1.4:16167226-16167245	MS.gene59101:CDS	35.0%
!	AAAGTGCTCCAATTTCATTC+TGG	+	chr1.4:16167128-16167147	MS.gene59101:intron	35.0%
!	CTTAGTGAAGGTTTTTGCTT+TGG	-	chr1.4:16166884-16166903	None:intergenic	35.0%
!	TAATGAAGTAGTGGAGTTCT+TGG	-	chr1.4:16166909-16166928	None:intergenic	35.0%
!!!	TTTTTGCTTTGGAGGAAGAT+TGG	-	chr1.4:16166873-16166892	None:intergenic	35.0%
	AAACAGACAGAGCATACTTG+AGG	-	chr1.4:16167027-16167046	None:intergenic	40.0%
	AAGAAGAAGGAGGAGAATCT+GGG	-	chr1.4:16166838-16166857	None:intergenic	40.0%
	GAGTTCTTGGATCTTAGTGA+AGG	-	chr1.4:16166896-16166915	None:intergenic	40.0%
	GTTTGGAGGTAATGAAGTAG+TGG	-	chr1.4:16166918-16166937	None:intergenic	40.0%
	GTTTGTTTGGGTGAAGAAGA+AGG	-	chr1.4:16166851-16166870	None:intergenic	40.0%
	TAACACCAGTAAGTAGCAAC+TGG	+	chr1.4:16167321-16167340	MS.gene59101:intron	40.0%
	TCAATTTGCATGAGCGAAGA+TGG	-	chr1.4:16166962-16166981	None:intergenic	40.0%
!	ACCACGATGGATTTGTTCTT+GGG	+	chr1.4:16167211-16167230	MS.gene59101:CDS	40.0%
!	AGTGAAGGTTTTTGCTTTGG+AGG	-	chr1.4:16166881-16166900	None:intergenic	40.0%
!	TTGCTACTTACTGGTGTTAG+TGG	-	chr1.4:16167320-16167339	None:intergenic	40.0%
!!	GGAGGAAGATTGGTTTGTTT+GGG	-	chr1.4:16166863-16166882	None:intergenic	40.0%
!!	TGGAGGAAGATTGGTTTGTT+TGG	-	chr1.4:16166864-16166883	None:intergenic	40.0%
	AGACGTAAAGAAGACCACGA+TGG	+	chr1.4:16167198-16167217	MS.gene59101:CDS	45.0%
	ATGCAAATTGAACGTGCCCT+TGG	+	chr1.4:16166971-16166990	MS.gene59101:CDS	45.0%
	CAGAAAAAGCTGAGGGAAAC+AGG	+	chr1.4:16167256-16167275	MS.gene59101:CDS	45.0%
	GAAGAAGAAGGAGGAGAATC+TGG	-	chr1.4:16166839-16166858	None:intergenic	45.0%
	GACTGTGCAGAAAAAGCTGA+GGG	+	chr1.4:16167249-16167268	MS.gene59101:CDS	45.0%
	TCCCAAGAACAAATCCATCG+TGG	-	chr1.4:16167215-16167234	None:intergenic	45.0%
	TGTTTGGGTGAAGAAGAAGG+AGG	-	chr1.4:16166848-16166867	None:intergenic	45.0%
!	AAGGAGGAGAATCTGGGTTT+TGG	-	chr1.4:16166832-16166851	None:intergenic	45.0%
!	AGGAGGAGAATCTGGGTTTT+GGG	-	chr1.4:16166831-16166850	None:intergenic	45.0%
!	CGTTACCAGTTGCTACTTAC+TGG	-	chr1.4:16167329-16167348	None:intergenic	45.0%
!	GACCACGATGGATTTGTTCT+TGG	+	chr1.4:16167210-16167229	MS.gene59101:CDS	45.0%
	AGAGTGGCTGGGTGAAAATG+GGG	+	chr1.4:16167279-16167298	MS.gene59101:CDS	50.0%
	GACAGAGCATACTTGAGGTC+AGG	-	chr1.4:16167022-16167041	None:intergenic	50.0%
	GAGAGTGGCTGGGTGAAAAT+GGG	+	chr1.4:16167278-16167297	MS.gene59101:CDS	50.0%
	GGACTGTGCAGAAAAAGCTG+AGG	+	chr1.4:16167248-16167267	MS.gene59101:CDS	50.0%
	GGTGTTAGTGGAGCGAAATC+TGG	-	chr1.4:16167308-16167327	None:intergenic	50.0%
	TGCATGAGCGAAGATGGTGA+TGG	-	chr1.4:16166956-16166975	None:intergenic	50.0%
!	GGAGGAGAATCTGGGTTTTG+GGG	-	chr1.4:16166830-16166849	None:intergenic	50.0%
	AGCTGAGGGAAACAGGAGAG+TGG	+	chr1.4:16167263-16167282	MS.gene59101:CDS	55.0%
	AGGGAAACAGGAGAGTGGCT+GGG	+	chr1.4:16167268-16167287	MS.gene59101:CDS	55.0%
	GGAGAGTGGCTGGGTGAAAA+TGG	+	chr1.4:16167277-16167296	MS.gene59101:CDS	55.0%
!	TCTGAAGCTACCAGCGCCAA+GGG	-	chr1.4:16166990-16167009	None:intergenic	55.0%
!!	CGAAGATGGTGATGGTCGAC+GGG	-	chr1.4:16166948-16166967	None:intergenic	55.0%
!!	GAAGATGGTGATGGTCGACG+GGG	-	chr1.4:16166947-16166966	None:intergenic	55.0%
!!	GCGAAGATGGTGATGGTCGA+CGG	-	chr1.4:16166949-16166968	None:intergenic	55.0%
	ATTGAACGTGCCCTTGGCGC+TGG	+	chr1.4:16166977-16166996	MS.gene59101:CDS	60.0%
	GAGGGAAACAGGAGAGTGGC+TGG	+	chr1.4:16167267-16167286	MS.gene59101:CDS	60.0%
!	CGACGGGGAAGCTTGTTTGG+AGG	-	chr1.4:16166932-16166951	None:intergenic	60.0%
!	GGTCGACGGGGAAGCTTGTT+TGG	-	chr1.4:16166935-16166954	None:intergenic	60.0%
!!	CTCTGAAGCTACCAGCGCCA+AGG	-	chr1.4:16166991-16167010	None:intergenic	60.0%
!!	GGCGCTGGTAGCTTCAGAGA+CGG	+	chr1.4:16166992-16167011	MS.gene59101:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	16166764	16167447	16166764	ID=MS.gene59101
chr1.4	mRNA	16166764	16167447	16166764	ID=MS.gene59101.t1;Parent=MS.gene59101
chr1.4	exon	16166764	16167029	16166764	ID=MS.gene59101.t1.exon1;Parent=MS.gene59101.t1
chr1.4	CDS	16166764	16167029	16166764	ID=cds.MS.gene59101.t1;Parent=MS.gene59101.t1
chr1.4	exon	16167183	16167328	16167183	ID=MS.gene59101.t1.exon2;Parent=MS.gene59101.t1
chr1.4	CDS	16167183	16167328	16167183	ID=cds.MS.gene59101.t1;Parent=MS.gene59101.t1
chr1.4	exon	16167410	16167447	16167410	ID=MS.gene59101.t1.exon3;Parent=MS.gene59101.t1
chr1.4	CDS	16167410	16167447	16167410	ID=cds.MS.gene59101.t1;Parent=MS.gene59101.t1

Gene Sequence

>MS.gene59101

ATGTTTTGCTTATGCAACAATCTTTCTATAACCAAACCCATTCTTATTACTAATGCAACTCTTCCCCAAAACCCAGATTCTCCTCCTTCTTCTTCACCCAAACAAACCAATCTTCCTCCAAAGCAAAAACCTTCACTAAGATCCAAGAACTCCACTACTTCATTACCTCCAAACAAGCTTCCCCGTCGACCATCACCATCTTCGCTCATGCAAATTGAACGTGCCCTTGGCGCTGGTAGCTTCAGAGACGGTGAACCTGACCTCAAGTATGCTCTGTCTGTTTGAATAAACAACTTATTTTGCAGCTTATTATAACATTATGATGAATAGGCTATTTCTATTCACTACCAATTTCATAATGTTAAAAGTGCTCCAATTTCATTCTGGATTTTGATTTCTCTGCATTTTTTTTTTAAAAGAATGAAGAACGATTCAGACGTAAAGAAGACCACGATGGATTTGTTCTTGGGACAAGTATTTGAAGGGACTGTGCAGAAAAAGCTGAGGGAAACAGGAGAGTGGCTGGGTGAAAATGGGGAGACCAGATTTCGCTCCACTAACACCAGTAAGTAGCAACTGGTAACGACTTTTCCGTATTTTTTGCCTCTTTAACAAGGATTGTAGCATGTCTTTTGCTTTGTTATAGGTGATTATGAGTGGGGAAAAACAGAAGTTATAGAGTAA

Protein sequence

>MS.gene59101.t1

MFCLCNNLSITKPILITNATLPQNPDSPPSSSPKQTNLPPKQKPSLRSKNSTTSLPPNKLPRRPSPSSLMQIERALGAGSFRDGEPDLKMKNDSDVKKTTMDLFLGQVFEGTVQKKLRETGEWLGENGETRFRSTNTSDYEWGKTEVIE