AlfalfaGEDB | MS.gene62955

M. sativa cultivar XinJiangDaYe / MS.gene62955

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62955.t1	XP_003589664.1	76.2	143	28	1	1	137	1	143	1.30E-48	202.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62955.t1	G7IA04	76.2	143	28	1	1	137	1	143	9.2e-49	202.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049137	MS.gene62955	0.826119	3.23E-54	-1.69E-46
MS.gene049171	MS.gene62955	0.848789	4.90E-60	-1.69E-46
MS.gene049257	MS.gene62955	0.801852	7.41E-49	-1.69E-46
MS.gene049285	MS.gene62955	0.803263	3.79E-49	-1.69E-46
MS.gene050315	MS.gene62955	0.805413	1.35E-49	-1.69E-46
MS.gene050507	MS.gene62955	0.803845	2.87E-49	-1.69E-46
MS.gene050577	MS.gene62955	0.819351	1.22E-52	-1.69E-46
MS.gene050699	MS.gene62955	0.824804	6.62E-54	-1.69E-46
MS.gene050747	MS.gene62955	0.800967	1.12E-48	-1.69E-46
MS.gene050781	MS.gene62955	0.819036	1.43E-52	-1.69E-46
MS.gene05081	MS.gene62955	0.82145	4.01E-53	-1.69E-46
MS.gene050950	MS.gene62955	0.803429	3.50E-49	-1.69E-46
MS.gene051171	MS.gene62955	0.817729	2.83E-52	-1.69E-46
MS.gene051244	MS.gene62955	0.846217	2.50E-59	-1.69E-46
MS.gene051666	MS.gene62955	0.806306	8.77E-50	-1.69E-46
MS.gene051838	MS.gene62955	0.808428	3.11E-50	-1.69E-46
MS.gene051973	MS.gene62955	-0.822252	2.62E-53	-1.69E-46
MS.gene052501	MS.gene62955	0.818768	1.65E-52	-1.69E-46
MS.gene052503	MS.gene62955	0.808847	2.53E-50	-1.69E-46
MS.gene052505	MS.gene62955	0.810034	1.41E-50	-1.69E-46
MS.gene052615	MS.gene62955	0.807113	5.92E-50	-1.69E-46
MS.gene052823	MS.gene62955	0.807512	4.87E-50	-1.69E-46
MS.gene052825	MS.gene62955	0.813099	3.04E-51	-1.69E-46
MS.gene053330	MS.gene62955	0.836794	7.59E-57	-1.69E-46
MS.gene053480	MS.gene62955	0.800036	1.74E-48	-1.69E-46
MS.gene053579	MS.gene62955	0.812436	4.24E-51	-1.69E-46
MS.gene053824	MS.gene62955	0.802568	5.27E-49	-1.69E-46
MS.gene054343	MS.gene62955	0.861386	1.06E-63	-1.69E-46
MS.gene054344	MS.gene62955	0.820265	7.52E-53	-1.69E-46
MS.gene05470	MS.gene62955	0.852075	5.85E-61	-1.69E-46
MS.gene054884	MS.gene62955	-0.847136	1.40E-59	-1.69E-46
MS.gene055206	MS.gene62955	0.803676	3.11E-49	-1.69E-46
MS.gene055370	MS.gene62955	0.871406	7.00E-67	-1.69E-46
MS.gene055502	MS.gene62955	0.805335	1.40E-49	-1.69E-46
MS.gene055577	MS.gene62955	0.805457	1.32E-49	-1.69E-46
MS.gene056351	MS.gene62955	0.825971	3.50E-54	-1.69E-46
MS.gene056633	MS.gene62955	0.880603	4.79E-70	-1.69E-46
MS.gene056664	MS.gene62955	0.822299	2.55E-53	-1.69E-46
MS.gene056877	MS.gene62955	0.818109	2.33E-52	-1.69E-46
MS.gene056988	MS.gene62955	-0.816257	6.07E-52	-1.69E-46
MS.gene057048	MS.gene62955	0.826699	2.35E-54	-1.69E-46
MS.gene057196	MS.gene62955	0.89615	4.86E-76	-1.69E-46
MS.gene057332	MS.gene62955	0.862579	4.59E-64	-1.69E-46
MS.gene057658	MS.gene62955	0.877653	5.29E-69	-1.69E-46
MS.gene057831	MS.gene62955	0.81714	3.85E-52	-1.69E-46
MS.gene058076	MS.gene62955	-0.809801	1.58E-50	-1.69E-46
MS.gene058233	MS.gene62955	0.807898	4.04E-50	-1.69E-46
MS.gene058418	MS.gene62955	-0.81029	1.24E-50	-1.69E-46
MS.gene058419	MS.gene62955	0.825572	4.36E-54	-1.69E-46
MS.gene058544	MS.gene62955	0.854094	1.55E-61	-1.69E-46
MS.gene058746	MS.gene62955	0.872823	2.36E-67	-1.69E-46
MS.gene058838	MS.gene62955	0.846561	2.01E-59	-1.69E-46
MS.gene059023	MS.gene62955	0.858894	6.02E-63	-1.69E-46
MS.gene05914	MS.gene62955	0.892198	1.98E-74	-1.69E-46
MS.gene05934	MS.gene62955	0.804586	2.01E-49	-1.69E-46
MS.gene059512	MS.gene62955	0.847693	9.84E-60	-1.69E-46
MS.gene059677	MS.gene62955	0.831324	1.78E-55	-1.69E-46
MS.gene059756	MS.gene62955	0.838537	2.71E-57	-1.69E-46
MS.gene06077	MS.gene62955	0.808886	2.48E-50	-1.69E-46
MS.gene06092	MS.gene62955	0.848054	7.82E-60	-1.69E-46
MS.gene061150	MS.gene62955	0.842036	3.30E-58	-1.69E-46
MS.gene061231	MS.gene62955	0.826273	2.97E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62955.t1	MTR_1g031740	84.615	143	16	1	1	137	1	143	1.85e-78	230

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 43 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGAGGAGAATCTGGGTTT+TGG	0.221469	1.3:-16905237	None:intergenic
GGAGAGTGGCTGGGTGAAAA+TGG	0.232098	1.3:+16905684	MS.gene62955:CDS
GAAGAAGAAGGAGGAGAATC+TGG	0.270359	1.3:-16905244	None:intergenic
TGGAGGAAGATTGGTTTGTT+TGG	0.288556	1.3:-16905269	None:intergenic
GGTCGACGGGGAAGCTTGTT+TGG	0.309086	1.3:-16905340	None:intergenic
GGAGGAAGATTGGTTTGTTT+GGG	0.355312	1.3:-16905268	None:intergenic
GACCACGATGGATTTGTTCT+TGG	0.365556	1.3:+16905617	MS.gene62955:CDS
TAATGAAGTAGTGGAGTTCT+TGG	0.377298	1.3:-16905314	None:intergenic
ACCACGATGGATTTGTTCTT+GGG	0.379690	1.3:+16905618	MS.gene62955:CDS
GAGAGTGGCTGGGTGAAAAT+GGG	0.391269	1.3:+16905685	MS.gene62955:CDS
GGTGTTAGTGGAGCGAAATC+TGG	0.391808	1.3:-16905712	None:intergenic
ATTAGTAATAAGAATGGGTT+TGG	0.396058	1.3:-16905203	None:intergenic
TTGCTACTTACTGGTGTTAG+TGG	0.404594	1.3:-16905724	None:intergenic
CGAAGATGGTGATGGTCGAC+GGG	0.447807	1.3:-16905353	None:intergenic
GAGGGAAACAGGAGAGTGGC+TGG	0.452230	1.3:+16905674	MS.gene62955:CDS
TCAATTTGCATGAGCGAAGA+TGG	0.459616	1.3:-16905367	None:intergenic
AAGAAGAAGGAGGAGAATCT+GGG	0.467707	1.3:-16905243	None:intergenic
GTTGCATTAGTAATAAGAAT+GGG	0.473568	1.3:-16905208	None:intergenic
GTTTGTTTGGGTGAAGAAGA+AGG	0.476332	1.3:-16905256	None:intergenic
AGTTGCATTAGTAATAAGAA+TGG	0.478851	1.3:-16905209	None:intergenic
TTCTTGGGACAAGTATTTGA+AGG	0.485835	1.3:+16905633	MS.gene62955:CDS
ATTGAACGTGCCCTTGGCGC+TGG	0.500974	1.3:+16905385	MS.gene62955:CDS
ATGCAAATTGAACGTGCCCT+TGG	0.511985	1.3:+16905379	MS.gene62955:CDS
GAGCATGCATACTTGAGGTC+AGG	0.520818	1.3:-16905427	None:intergenic
TGTTATAGGTGATTATGAGT+GGG	0.521671	1.3:+16905809	MS.gene62955:intron
CTCTGAAGCTACCAGCGCCA+AGG	0.529782	1.3:-16905396	None:intergenic
GTTATAGGTGATTATGAGTG+GGG	0.532217	1.3:+16905810	MS.gene62955:intron
GCGAAGATGGTGATGGTCGA+CGG	0.545160	1.3:-16905354	None:intergenic
TCTTGGGACAAGTATTTGAA+GGG	0.567555	1.3:+16905634	MS.gene62955:CDS
GAGTTCTTGGATCTTAGTGA+AGG	0.568920	1.3:-16905301	None:intergenic
CGACGGGGAAGCTTGTTTGG+AGG	0.576924	1.3:-16905337	None:intergenic
AGAGTGGCTGGGTGAAAATG+GGG	0.590303	1.3:+16905686	MS.gene62955:CDS
TCTGAAGCTACCAGCGCCAA+GGG	0.590855	1.3:-16905395	None:intergenic
TTGTTATAGGTGATTATGAG+TGG	0.595225	1.3:+16905808	MS.gene62955:intron
GTTTGGAGGTAATGAAGTAG+TGG	0.605102	1.3:-16905323	None:intergenic
TGTTTGGGTGAAGAAGAAGG+AGG	0.610626	1.3:-16905253	None:intergenic
AGGGAAACAGGAGAGTGGCT+GGG	0.620803	1.3:+16905675	MS.gene62955:CDS
GGCGCTGGTAGCTTCAGAGA+CGG	0.635842	1.3:+16905400	MS.gene62955:CDS
AGCTGAGGGAAACAGGAGAG+TGG	0.671817	1.3:+16905670	MS.gene62955:CDS
GAAGATGGTGATGGTCGACG+GGG	0.719959	1.3:-16905352	None:intergenic
TGCATGAGCGAAGATGGTGA+TGG	0.732147	1.3:-16905361	None:intergenic
TCCCAAGAACAAATCCATCG+TGG	0.736351	1.3:-16905619	None:intergenic
AGACGTAAAGAAGACCACGA+TGG	0.745068	1.3:+16905605	MS.gene62955:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTATAACATTATGATGAAT+AGG	+	chr1.3:16905480-16905499	MS.gene62955:intron	15.0%
!!!	CACTTTTAACATTATGAAAT+TGG	-	chr1.3:16905522-16905541	None:intergenic	20.0%
!	AGTTGCATTAGTAATAAGAA+TGG	-	chr1.3:16905212-16905231	None:intergenic	25.0%
!	ATCAAAATCCAGAATGAAAT+TGG	-	chr1.3:16905547-16905566	None:intergenic	25.0%
!	GTTGCATTAGTAATAAGAAT+GGG	-	chr1.3:16905211-16905230	None:intergenic	25.0%
!	TGTTAAAGAGGCAAAAAATA+CGG	-	chr1.3:16905765-16905784	None:intergenic	25.0%
!!	ATTAGTAATAAGAATGGGTT+TGG	-	chr1.3:16905206-16905225	None:intergenic	25.0%
!!!	TATTTTTTGCCTCTTTAACA+AGG	+	chr1.3:16905765-16905784	MS.gene62955:intron	25.0%
	TGTTATAGGTGATTATGAGT+GGG	+	chr1.3:16905809-16905828	MS.gene62955:intron	30.0%
	TTGTTATAGGTGATTATGAG+TGG	+	chr1.3:16905808-16905827	MS.gene62955:intron	30.0%
!!	CATGTCTTTTGCTTTGTTAT+AGG	+	chr1.3:16905795-16905814	MS.gene62955:intron	30.0%
	GTTATAGGTGATTATGAGTG+GGG	+	chr1.3:16905810-16905829	MS.gene62955:intron	35.0%
	TCTTGGGACAAGTATTTGAA+GGG	+	chr1.3:16905634-16905653	MS.gene62955:CDS	35.0%
	TGCTACAATCCTTGTTAAAG+AGG	-	chr1.3:16905777-16905796	None:intergenic	35.0%
	TTCTTGGGACAAGTATTTGA+AGG	+	chr1.3:16905633-16905652	MS.gene62955:CDS	35.0%
!	AAAGTGCTCCAATTTCATTC+TGG	+	chr1.3:16905536-16905555	MS.gene62955:intron	35.0%
!	CTTAGTGAAGGTTTTTGCTT+TGG	-	chr1.3:16905292-16905311	None:intergenic	35.0%
!	TAATGAAGTAGTGGAGTTCT+TGG	-	chr1.3:16905317-16905336	None:intergenic	35.0%
!!!	TTTTTGCTTTGGAGGAAGAT+TGG	-	chr1.3:16905281-16905300	None:intergenic	35.0%
	AAACAGAGCATGCATACTTG+AGG	-	chr1.3:16905435-16905454	None:intergenic	40.0%
	AAGAAGAAGGAGGAGAATCT+GGG	-	chr1.3:16905246-16905265	None:intergenic	40.0%
	GAGTTCTTGGATCTTAGTGA+AGG	-	chr1.3:16905304-16905323	None:intergenic	40.0%
	GTTTGGAGGTAATGAAGTAG+TGG	-	chr1.3:16905326-16905345	None:intergenic	40.0%
	GTTTGTTTGGGTGAAGAAGA+AGG	-	chr1.3:16905259-16905278	None:intergenic	40.0%
	TAACACCAGTAAGTAGCAAC+TGG	+	chr1.3:16905728-16905747	MS.gene62955:intron	40.0%
	TCAATTTGCATGAGCGAAGA+TGG	-	chr1.3:16905370-16905389	None:intergenic	40.0%
!	ACCACGATGGATTTGTTCTT+GGG	+	chr1.3:16905618-16905637	MS.gene62955:CDS	40.0%
!	AGTGAAGGTTTTTGCTTTGG+AGG	-	chr1.3:16905289-16905308	None:intergenic	40.0%
!	TTGCTACTTACTGGTGTTAG+TGG	-	chr1.3:16905727-16905746	None:intergenic	40.0%
!!	GGAGGAAGATTGGTTTGTTT+GGG	-	chr1.3:16905271-16905290	None:intergenic	40.0%
!!	TGGAGGAAGATTGGTTTGTT+TGG	-	chr1.3:16905272-16905291	None:intergenic	40.0%
	AGACGTAAAGAAGACCACGA+TGG	+	chr1.3:16905605-16905624	MS.gene62955:CDS	45.0%
	ATGCAAATTGAACGTGCCCT+TGG	+	chr1.3:16905379-16905398	MS.gene62955:CDS	45.0%
	CAGAAAAAGCTGAGGGAAAC+AGG	+	chr1.3:16905663-16905682	MS.gene62955:CDS	45.0%
	GAAGAAGAAGGAGGAGAATC+TGG	-	chr1.3:16905247-16905266	None:intergenic	45.0%
	GACTGTGCAGAAAAAGCTGA+GGG	+	chr1.3:16905656-16905675	MS.gene62955:CDS	45.0%
	TCCCAAGAACAAATCCATCG+TGG	-	chr1.3:16905622-16905641	None:intergenic	45.0%
	TGTTTGGGTGAAGAAGAAGG+AGG	-	chr1.3:16905256-16905275	None:intergenic	45.0%
!	AAGGAGGAGAATCTGGGTTT+TGG	-	chr1.3:16905240-16905259	None:intergenic	45.0%
!	AGGAGGAGAATCTGGGTTTT+GGG	-	chr1.3:16905239-16905258	None:intergenic	45.0%
!	CGTTACCAGTTGCTACTTAC+TGG	-	chr1.3:16905736-16905755	None:intergenic	45.0%
!	GACCACGATGGATTTGTTCT+TGG	+	chr1.3:16905617-16905636	MS.gene62955:CDS	45.0%
	AGAGTGGCTGGGTGAAAATG+GGG	+	chr1.3:16905686-16905705	MS.gene62955:CDS	50.0%
	GAGAGTGGCTGGGTGAAAAT+GGG	+	chr1.3:16905685-16905704	MS.gene62955:CDS	50.0%
	GAGCATGCATACTTGAGGTC+AGG	-	chr1.3:16905430-16905449	None:intergenic	50.0%
	GGACTGTGCAGAAAAAGCTG+AGG	+	chr1.3:16905655-16905674	MS.gene62955:CDS	50.0%
	GGTGTTAGTGGAGCGAAATC+TGG	-	chr1.3:16905715-16905734	None:intergenic	50.0%
	TGCATGAGCGAAGATGGTGA+TGG	-	chr1.3:16905364-16905383	None:intergenic	50.0%
!	GGAGGAGAATCTGGGTTTTG+GGG	-	chr1.3:16905238-16905257	None:intergenic	50.0%
	AGCTGAGGGAAACAGGAGAG+TGG	+	chr1.3:16905670-16905689	MS.gene62955:CDS	55.0%
	AGGGAAACAGGAGAGTGGCT+GGG	+	chr1.3:16905675-16905694	MS.gene62955:CDS	55.0%
	GGAGAGTGGCTGGGTGAAAA+TGG	+	chr1.3:16905684-16905703	MS.gene62955:CDS	55.0%
!	TCTGAAGCTACCAGCGCCAA+GGG	-	chr1.3:16905398-16905417	None:intergenic	55.0%
!!	CGAAGATGGTGATGGTCGAC+GGG	-	chr1.3:16905356-16905375	None:intergenic	55.0%
!!	GAAGATGGTGATGGTCGACG+GGG	-	chr1.3:16905355-16905374	None:intergenic	55.0%
!!	GCGAAGATGGTGATGGTCGA+CGG	-	chr1.3:16905357-16905376	None:intergenic	55.0%
	ATTGAACGTGCCCTTGGCGC+TGG	+	chr1.3:16905385-16905404	MS.gene62955:CDS	60.0%
	GAGGGAAACAGGAGAGTGGC+TGG	+	chr1.3:16905674-16905693	MS.gene62955:CDS	60.0%
!	CGACGGGGAAGCTTGTTTGG+AGG	-	chr1.3:16905340-16905359	None:intergenic	60.0%
!	GGTCGACGGGGAAGCTTGTT+TGG	-	chr1.3:16905343-16905362	None:intergenic	60.0%
!!	CTCTGAAGCTACCAGCGCCA+AGG	-	chr1.3:16905399-16905418	None:intergenic	60.0%
!!	GGCGCTGGTAGCTTCAGAGA+CGG	+	chr1.3:16905400-16905419	MS.gene62955:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	16905172	16905854	16905172	ID=MS.gene62955
chr1.3	mRNA	16905172	16905854	16905172	ID=MS.gene62955.t1;Parent=MS.gene62955
chr1.3	exon	16905172	16905437	16905172	ID=MS.gene62955.t1.exon1;Parent=MS.gene62955.t1
chr1.3	CDS	16905172	16905437	16905172	ID=cds.MS.gene62955.t1;Parent=MS.gene62955.t1
chr1.3	exon	16905590	16905735	16905590	ID=MS.gene62955.t1.exon2;Parent=MS.gene62955.t1
chr1.3	CDS	16905590	16905735	16905590	ID=cds.MS.gene62955.t1;Parent=MS.gene62955.t1
chr1.3	exon	16905817	16905854	16905817	ID=MS.gene62955.t1.exon3;Parent=MS.gene62955.t1
chr1.3	CDS	16905817	16905854	16905817	ID=cds.MS.gene62955.t1;Parent=MS.gene62955.t1

Gene Sequence

>MS.gene62955

ATGTTTTGCTTATGCAACAATCTTTCTATAACCAAACCCATTCTTATTACTAATGCAACTCTTCCCCAAAACCCAGATTCTCCTCCTTCTTCTTCACCCAAACAAACCAATCTTCCTCCAAAGCAAAAACCTTCACTAAGATCCAAGAACTCCACTACTTCATTACCTCCAAACAAGCTTCCCCGTCGACCATCACCATCTTCGCTCATGCAAATTGAACGTGCCCTTGGCGCTGGTAGCTTCAGAGACGGTGAACCTGACCTCAAGTATGCATGCTCTGTTTGAATAAACAACTTATTTTGCAGCTTATTATAACATTATGATGAATAGGCTATTTCTATTCACTACCAATTTCATAATGTTAAAAGTGCTCCAATTTCATTCTGGATTTTGATTTTTCTGCATTTTTTTTTTAAAGAATGAAGAACGATTCAGACGTAAAGAAGACCACGATGGATTTGTTCTTGGGACAAGTATTTGAAGGGACTGTGCAGAAAAAGCTGAGGGAAACAGGAGAGTGGCTGGGTGAAAATGGGGAGACCAGATTTCGCTCCACTAACACCAGTAAGTAGCAACTGGTAACGACTTTTCCGTATTTTTTGCCTCTTTAACAAGGATTGTAGCATGTCTTTTGCTTTGTTATAGGTGATTATGAGTGGGGAAAAACAGAAGTTATAGAGTAA

Protein sequence

>MS.gene62955.t1

MFCLCNNLSITKPILITNATLPQNPDSPPSSSPKQTNLPPKQKPSLRSKNSTTSLPPNKLPRRPSPSSLMQIERALGAGSFRDGEPDLKMKNDSDVKKTTMDLFLGQVFEGTVQKKLRETGEWLGENGETRFRSTNTSDYEWGKTEVIE