Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72416.t1 | XP_013468054.1 | 79.4 | 218 | 3 | 1 | 1 | 176 | 1 | 218 | 1.80E-89 | 338.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72416.t1 | O81864 | 83.6 | 146 | 24 | 0 | 29 | 174 | 80 | 225 | 3.9e-69 | 262.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72416.t1 | A0A072VKD1 | 79.4 | 218 | 3 | 1 | 1 | 176 | 1 | 218 | 1.3e-89 | 338.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene72416 | 0.809243 | 2.08E-50 | -1.69E-46 |
| MS.gene049171 | MS.gene72416 | 0.876249 | 1.62E-68 | -1.69E-46 |
| MS.gene049222 | MS.gene72416 | 0.825741 | 3.97E-54 | -1.69E-46 |
| MS.gene049257 | MS.gene72416 | 0.83707 | 6.46E-57 | -1.69E-46 |
| MS.gene049285 | MS.gene72416 | 0.818679 | 1.73E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene72416 | 0.80038 | 1.48E-48 | -1.69E-46 |
| MS.gene049373 | MS.gene72416 | 0.800113 | 1.68E-48 | -1.69E-46 |
| MS.gene049609 | MS.gene72416 | 0.830036 | 3.68E-55 | -1.69E-46 |
| MS.gene049633 | MS.gene72416 | 0.839284 | 1.74E-57 | -1.69E-46 |
| MS.gene050170 | MS.gene72416 | 0.861974 | 7.03E-64 | -1.69E-46 |
| MS.gene050315 | MS.gene72416 | 0.827402 | 1.60E-54 | -1.69E-46 |
| MS.gene050323 | MS.gene72416 | 0.804445 | 2.15E-49 | -1.69E-46 |
| MS.gene050479 | MS.gene72416 | -0.811295 | 7.52E-51 | -1.69E-46 |
| MS.gene050496 | MS.gene72416 | 0.80224 | 6.16E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene72416 | 0.824967 | 6.06E-54 | -1.69E-46 |
| MS.gene050538 | MS.gene72416 | 0.829007 | 6.55E-55 | -1.69E-46 |
| MS.gene050547 | MS.gene72416 | -0.811277 | 7.59E-51 | -1.69E-46 |
| MS.gene050577 | MS.gene72416 | 0.859945 | 2.91E-63 | -1.69E-46 |
| MS.gene050612 | MS.gene72416 | 0.826958 | 2.04E-54 | -1.69E-46 |
| MS.gene050699 | MS.gene72416 | 0.836639 | 8.32E-57 | -1.69E-46 |
| MS.gene050747 | MS.gene72416 | 0.839202 | 1.82E-57 | -1.69E-46 |
| MS.gene050772 | MS.gene72416 | 0.803481 | 3.41E-49 | -1.69E-46 |
| MS.gene05080 | MS.gene72416 | 0.832508 | 9.08E-56 | -1.69E-46 |
| MS.gene05081 | MS.gene72416 | 0.897348 | 1.53E-76 | -1.69E-46 |
| MS.gene050868 | MS.gene72416 | 0.832667 | 8.30E-56 | -1.69E-46 |
| MS.gene050871 | MS.gene72416 | 0.829618 | 4.65E-55 | -1.69E-46 |
| MS.gene050904 | MS.gene72416 | 0.825111 | 5.60E-54 | -1.69E-46 |
| MS.gene050950 | MS.gene72416 | 0.835301 | 1.82E-56 | -1.69E-46 |
| MS.gene050986 | MS.gene72416 | 0.809259 | 2.07E-50 | -1.69E-46 |
| MS.gene051138 | MS.gene72416 | -0.807183 | 5.72E-50 | -1.69E-46 |
| MS.gene051317 | MS.gene72416 | 0.832634 | 8.45E-56 | -1.69E-46 |
| MS.gene051552 | MS.gene72416 | -0.825983 | 3.48E-54 | -1.69E-46 |
| MS.gene051838 | MS.gene72416 | 0.891503 | 3.74E-74 | -1.69E-46 |
| MS.gene051973 | MS.gene72416 | -0.839006 | 2.05E-57 | -1.69E-46 |
| MS.gene052015 | MS.gene72416 | -0.818673 | 1.73E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene72416 | 0.830791 | 2.41E-55 | -1.69E-46 |
| MS.gene052289 | MS.gene72416 | -0.811774 | 5.92E-51 | -1.69E-46 |
| MS.gene05240 | MS.gene72416 | -0.801516 | 8.68E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene72416 | 0.817495 | 3.20E-52 | -1.69E-46 |
| MS.gene052486 | MS.gene72416 | 0.816469 | 5.44E-52 | -1.69E-46 |
| MS.gene052494 | MS.gene72416 | -0.809523 | 1.81E-50 | -1.69E-46 |
| MS.gene052501 | MS.gene72416 | 0.830431 | 2.95E-55 | -1.69E-46 |
| MS.gene052503 | MS.gene72416 | 0.852753 | 3.75E-61 | -1.69E-46 |
| MS.gene052505 | MS.gene72416 | 0.822015 | 2.97E-53 | -1.69E-46 |
| MS.gene052606 | MS.gene72416 | -0.834671 | 2.62E-56 | -1.69E-46 |
| MS.gene052615 | MS.gene72416 | 0.821038 | 4.99E-53 | -1.69E-46 |
| MS.gene052823 | MS.gene72416 | 0.850578 | 1.55E-60 | -1.69E-46 |
| MS.gene052825 | MS.gene72416 | 0.856351 | 3.41E-62 | -1.69E-46 |
| MS.gene052870 | MS.gene72416 | 0.810403 | 1.17E-50 | -1.69E-46 |
| MS.gene052872 | MS.gene72416 | 0.827485 | 1.52E-54 | -1.69E-46 |
| MS.gene053097 | MS.gene72416 | -0.813677 | 2.27E-51 | -1.69E-46 |
| MS.gene053203 | MS.gene72416 | 0.860589 | 1.86E-63 | -1.69E-46 |
| MS.gene053207 | MS.gene72416 | 0.807551 | 4.78E-50 | -1.69E-46 |
| MS.gene053421 | MS.gene72416 | -0.80429 | 2.32E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene72416 | 0.833538 | 5.04E-56 | -1.69E-46 |
| MS.gene053480 | MS.gene72416 | 0.853988 | 1.66E-61 | -1.69E-46 |
| MS.gene053579 | MS.gene72416 | 0.801251 | 9.83E-49 | -1.69E-46 |
| MS.gene053632 | MS.gene72416 | 0.811318 | 7.43E-51 | -1.69E-46 |
| MS.gene053824 | MS.gene72416 | 0.847621 | 1.03E-59 | -1.69E-46 |
| MS.gene054107 | MS.gene72416 | 0.815001 | 1.16E-51 | -1.69E-46 |
| MS.gene054343 | MS.gene72416 | 0.877983 | 4.06E-69 | -1.69E-46 |
| MS.gene054344 | MS.gene72416 | 0.886522 | 3.18E-72 | -1.69E-46 |
| MS.gene054408 | MS.gene72416 | 0.817265 | 3.61E-52 | -1.69E-46 |
| MS.gene054600 | MS.gene72416 | 0.822384 | 2.44E-53 | -1.69E-46 |
| MS.gene054706 | MS.gene72416 | -0.826738 | 2.30E-54 | -1.69E-46 |
| MS.gene05470 | MS.gene72416 | 0.889947 | 1.53E-73 | -1.69E-46 |
| MS.gene054742 | MS.gene72416 | -0.805321 | 1.41E-49 | -1.69E-46 |
| MS.gene054884 | MS.gene72416 | -0.863529 | 2.33E-64 | -1.69E-46 |
| MS.gene054927 | MS.gene72416 | 0.826171 | 3.14E-54 | -1.69E-46 |
| MS.gene054954 | MS.gene72416 | 0.811616 | 6.40E-51 | -1.69E-46 |
| MS.gene054959 | MS.gene72416 | -0.807466 | 4.99E-50 | -1.69E-46 |
| MS.gene055206 | MS.gene72416 | 0.843536 | 1.32E-58 | -1.69E-46 |
| MS.gene055289 | MS.gene72416 | 0.829709 | 4.42E-55 | -1.69E-46 |
| MS.gene055362 | MS.gene72416 | 0.827212 | 1.77E-54 | -1.69E-46 |
| MS.gene055370 | MS.gene72416 | 0.847338 | 1.23E-59 | -1.69E-46 |
| MS.gene055502 | MS.gene72416 | 0.842854 | 2.01E-58 | -1.69E-46 |
| MS.gene055577 | MS.gene72416 | 0.861964 | 7.09E-64 | -1.69E-46 |
| MS.gene055729 | MS.gene72416 | -0.801388 | 9.22E-49 | -1.69E-46 |
| MS.gene055730 | MS.gene72416 | -0.800204 | 1.61E-48 | -1.69E-46 |
| MS.gene055731 | MS.gene72416 | -0.822755 | 2.00E-53 | -1.69E-46 |
| MS.gene055748 | MS.gene72416 | 0.84348 | 1.37E-58 | -1.69E-46 |
| MS.gene055751 | MS.gene72416 | 0.828106 | 1.08E-54 | -1.69E-46 |
| MS.gene05589 | MS.gene72416 | 0.87688 | 9.82E-69 | -1.69E-46 |
| MS.gene055941 | MS.gene72416 | 0.809921 | 1.49E-50 | -1.69E-46 |
| MS.gene055977 | MS.gene72416 | -0.804318 | 2.29E-49 | -1.69E-46 |
| MS.gene056040 | MS.gene72416 | 0.803729 | 3.03E-49 | -1.69E-46 |
| MS.gene056351 | MS.gene72416 | 0.823743 | 1.18E-53 | -1.69E-46 |
| MS.gene056387 | MS.gene72416 | 0.807861 | 4.11E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene72416 | 0.839478 | 1.55E-57 | -1.69E-46 |
| MS.gene056590 | MS.gene72416 | -0.813269 | 2.79E-51 | -1.69E-46 |
| MS.gene056591 | MS.gene72416 | -0.814639 | 1.39E-51 | -1.69E-46 |
| MS.gene056633 | MS.gene72416 | 0.842572 | 2.38E-58 | -1.69E-46 |
| MS.gene056692 | MS.gene72416 | 0.832175 | 1.10E-55 | -1.69E-46 |
| MS.gene056694 | MS.gene72416 | -0.808388 | 3.17E-50 | -1.69E-46 |
| MS.gene056748 | MS.gene72416 | -0.805363 | 1.38E-49 | -1.69E-46 |
| MS.gene056877 | MS.gene72416 | 0.828658 | 7.95E-55 | -1.69E-46 |
| MS.gene056988 | MS.gene72416 | -0.842299 | 2.82E-58 | -1.69E-46 |
| MS.gene057048 | MS.gene72416 | 0.837284 | 5.69E-57 | -1.69E-46 |
| MS.gene057196 | MS.gene72416 | 0.85868 | 6.98E-63 | -1.69E-46 |
| MS.gene057332 | MS.gene72416 | 0.870991 | 9.60E-67 | -1.69E-46 |
| MS.gene057658 | MS.gene72416 | 0.892037 | 2.30E-74 | -1.69E-46 |
| MS.gene057831 | MS.gene72416 | 0.842683 | 2.23E-58 | -1.69E-46 |
| MS.gene058076 | MS.gene72416 | -0.814443 | 1.54E-51 | -1.69E-46 |
| MS.gene058157 | MS.gene72416 | 0.800763 | 1.24E-48 | -1.69E-46 |
| MS.gene058334 | MS.gene72416 | -0.833303 | 5.76E-56 | -1.69E-46 |
| MS.gene058338 | MS.gene72416 | 0.844489 | 7.32E-59 | -1.69E-46 |
| MS.gene05837 | MS.gene72416 | 0.848633 | 5.41E-60 | -1.69E-46 |
| MS.gene058418 | MS.gene72416 | -0.848011 | 8.04E-60 | -1.69E-46 |
| MS.gene058419 | MS.gene72416 | 0.831186 | 1.93E-55 | -1.69E-46 |
| MS.gene058444 | MS.gene72416 | 0.809819 | 1.57E-50 | -1.69E-46 |
| MS.gene058544 | MS.gene72416 | 0.872231 | 3.73E-67 | -1.69E-46 |
| MS.gene058603 | MS.gene72416 | -0.822218 | 2.66E-53 | -1.69E-46 |
| MS.gene058746 | MS.gene72416 | 0.830122 | 3.51E-55 | -1.69E-46 |
| MS.gene058838 | MS.gene72416 | 0.884737 | 1.49E-71 | -1.69E-46 |
| MS.gene059022 | MS.gene72416 | 0.825134 | 5.53E-54 | -1.69E-46 |
| MS.gene059023 | MS.gene72416 | 0.901903 | 1.67E-78 | -1.69E-46 |
| MS.gene059103 | MS.gene72416 | -0.813446 | 2.55E-51 | -1.69E-46 |
| MS.gene05914 | MS.gene72416 | 0.910949 | 1.06E-82 | -1.69E-46 |
| MS.gene059303 | MS.gene72416 | 0.85321 | 2.78E-61 | -1.69E-46 |
| MS.gene059480 | MS.gene72416 | -0.813876 | 2.05E-51 | -1.69E-46 |
| MS.gene059512 | MS.gene72416 | 0.866977 | 1.92E-65 | -1.69E-46 |
| MS.gene05960 | MS.gene72416 | -0.842971 | 1.87E-58 | -1.69E-46 |
| MS.gene059667 | MS.gene72416 | 0.832369 | 9.83E-56 | -1.69E-46 |
| MS.gene059677 | MS.gene72416 | 0.842681 | 2.23E-58 | -1.69E-46 |
| MS.gene059718 | MS.gene72416 | 0.830422 | 2.96E-55 | -1.69E-46 |
| MS.gene059756 | MS.gene72416 | 0.836534 | 8.84E-57 | -1.69E-46 |
| MS.gene059865 | MS.gene72416 | 0.804699 | 1.91E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene72416 | 0.829986 | 3.79E-55 | -1.69E-46 |
| MS.gene060087 | MS.gene72416 | -0.813822 | 2.11E-51 | -1.69E-46 |
| MS.gene06027 | MS.gene72416 | 0.820153 | 7.97E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene72416 | 0.854303 | 1.35E-61 | -1.69E-46 |
| MS.gene06077 | MS.gene72416 | 0.85362 | 2.12E-61 | -1.69E-46 |
| MS.gene06092 | MS.gene72416 | 0.855711 | 5.25E-62 | -1.69E-46 |
| MS.gene060982 | MS.gene72416 | 0.83395 | 3.97E-56 | -1.69E-46 |
| MS.gene061044 | MS.gene72416 | -0.812953 | 3.27E-51 | -1.69E-46 |
| MS.gene061150 | MS.gene72416 | 0.836568 | 8.67E-57 | -1.69E-46 |
| MS.gene061231 | MS.gene72416 | 0.84506 | 5.14E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72416.t1 | MTR_1g061080 | 79.358 | 218 | 3 | 1 | 1 | 176 | 1 | 218 | 6.17e-120 | 338 |
| MS.gene72416.t1 | MTR_7g053470 | 40.449 | 89 | 44 | 3 | 49 | 136 | 47 | 127 | 1.28e-13 | 68.2 |
| MS.gene72416.t1 | MTR_7g006570 | 32.680 | 153 | 88 | 4 | 25 | 171 | 92 | 235 | 7.92e-13 | 64.7 |
| MS.gene72416.t1 | MTR_8g080980 | 41.176 | 102 | 50 | 4 | 37 | 136 | 38 | 131 | 9.19e-13 | 65.9 |
| MS.gene72416.t1 | MTR_3g060750 | 30.935 | 139 | 79 | 3 | 35 | 173 | 83 | 204 | 1.04e-12 | 63.9 |
| MS.gene72416.t1 | MTR_1g061850 | 37.838 | 111 | 60 | 4 | 29 | 136 | 127 | 231 | 9.67e-12 | 62.0 |
| MS.gene72416.t1 | MTR_5g008680 | 35.537 | 121 | 45 | 3 | 54 | 174 | 413 | 500 | 1.32e-11 | 62.4 |
| MS.gene72416.t1 | MTR_8g006755 | 35.000 | 120 | 61 | 5 | 45 | 162 | 358 | 462 | 2.25e-11 | 61.6 |
| MS.gene72416.t1 | MTR_8g107010 | 35.398 | 113 | 56 | 5 | 32 | 135 | 134 | 238 | 3.68e-11 | 60.5 |
| MS.gene72416.t1 | MTR_8g012340 | 39.326 | 89 | 45 | 3 | 49 | 136 | 53 | 133 | 4.91e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72416.t1 | AT4G19830 | 82.993 | 147 | 25 | 0 | 29 | 175 | 80 | 226 | 1.25e-88 | 259 |
| MS.gene72416.t1 | AT4G26555 | 32.692 | 156 | 87 | 4 | 17 | 171 | 20 | 158 | 3.82e-17 | 74.7 |
| MS.gene72416.t1 | AT4G26555 | 32.692 | 156 | 87 | 4 | 17 | 171 | 66 | 204 | 5.16e-17 | 75.5 |
| MS.gene72416.t1 | AT5G48570 | 33.766 | 154 | 68 | 4 | 22 | 174 | 33 | 153 | 1.81e-15 | 73.6 |
| MS.gene72416.t1 | AT5G13410 | 34.211 | 152 | 87 | 5 | 25 | 171 | 108 | 251 | 4.09e-15 | 71.2 |
| MS.gene72416.t1 | AT3G25230 | 40.196 | 102 | 51 | 4 | 37 | 136 | 39 | 132 | 1.54e-13 | 68.2 |
| MS.gene72416.t1 | AT3G25230 | 40.196 | 102 | 51 | 4 | 37 | 136 | 39 | 132 | 1.56e-13 | 68.2 |
| MS.gene72416.t1 | AT5G05420 | 30.137 | 146 | 67 | 4 | 31 | 174 | 31 | 143 | 1.80e-12 | 62.0 |
| MS.gene72416.t1 | AT3G60370 | 31.298 | 131 | 57 | 4 | 45 | 175 | 129 | 226 | 7.28e-12 | 62.0 |
| MS.gene72416.t1 | AT3G60370 | 31.298 | 131 | 57 | 4 | 45 | 175 | 158 | 255 | 7.69e-12 | 62.4 |
| MS.gene72416.t1 | AT1G18170 | 39.474 | 114 | 56 | 5 | 32 | 136 | 121 | 230 | 1.62e-11 | 61.2 |
| MS.gene72416.t1 | AT3G10060 | 30.667 | 150 | 81 | 6 | 4 | 136 | 61 | 204 | 2.19e-11 | 60.8 |
Find 48 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGGTGAAGGTTCGACTTT+AGG | 0.241757 | 1.4:-50273567 | MS.gene72416:CDS |
| TTCTCTGAACTCCCCAATTC+TGG | 0.241954 | 1.4:-50274911 | MS.gene72416:CDS |
| TTTGTCTTTGTCCTTGGTTC+TGG | 0.255718 | 1.4:-50274451 | MS.gene72416:CDS |
| TTGGAACTCCTTGTTGGTTC+AGG | 0.280949 | 1.4:-50274875 | MS.gene72416:CDS |
| ATAATAAAATACTGTGTCTT+TGG | 0.282096 | 1.4:-50275109 | MS.gene72416:CDS |
| ATGGTGAAGGTTCGACTTTA+GGG | 0.294637 | 1.4:-50273566 | MS.gene72416:CDS |
| GGCCTTAACACCACCAGAAT+TGG | 0.297767 | 1.4:+50274898 | None:intergenic |
| TTCTGGTGGTGTTAAGGCCT+TGG | 0.355031 | 1.4:-50274894 | MS.gene72416:CDS |
| ATTCTCATCTTTGTGGTCAT+AGG | 0.366810 | 1.4:+50274489 | None:intergenic |
| GCCTTAACACCACCAGAATT+GGG | 0.386314 | 1.4:+50274899 | None:intergenic |
| CCCCAATTCTGGTGGTGTTA+AGG | 0.386678 | 1.4:-50274900 | MS.gene72416:CDS |
| TGCTGTGTTCAGGTAATTGC+TGG | 0.390936 | 1.4:-50273965 | MS.gene72416:intron |
| CTTTGTCCTTGGTTCTGGTA+AGG | 0.410071 | 1.4:-50274446 | MS.gene72416:intron |
| ACTTATATTTGATATTGAAC+TGG | 0.414468 | 1.4:-50273541 | MS.gene72416:CDS |
| GATCACCATTCTCATCTTTG+TGG | 0.422175 | 1.4:+50274482 | None:intergenic |
| AAGGCCTTGGAACTCCTTGT+TGG | 0.425790 | 1.4:-50274881 | MS.gene72416:CDS |
| AGGACAAAGACAAAAGGATT+AGG | 0.454496 | 1.4:+50274460 | None:intergenic |
| AAGGTTGAGATTCATTACTA+TGG | 0.463418 | 1.4:-50274550 | MS.gene72416:CDS |
| TTTCTTACATTAGGCGGGAT+AGG | 0.475564 | 1.4:+50273851 | None:intergenic |
| AATCCGACACGACTTGCAAA+TGG | 0.477408 | 1.4:-50273585 | MS.gene72416:CDS |
| GTCATCATACCTCCCTCACT+TGG | 0.492147 | 1.4:-50273896 | MS.gene72416:CDS |
| AAAAGTTCTTTGACAGGCAG+AGG | 0.504470 | 1.4:-50273626 | MS.gene72416:intron |
| AAGGATTTCTTACATTAGGC+GGG | 0.525661 | 1.4:+50273846 | None:intergenic |
| ATATTTGATATTGAACTGGT+TGG | 0.533481 | 1.4:-50273537 | MS.gene72416:CDS |
| AAAGGATTTCTTACATTAGG+CGG | 0.534022 | 1.4:+50273845 | None:intergenic |
| GAACCAAGGACAAAGACAAA+AGG | 0.544303 | 1.4:+50274454 | None:intergenic |
| TCAGGTGAAGTTCCTCAAGA+TGG | 0.562507 | 1.4:-50274857 | MS.gene72416:CDS |
| TCTGAACTCCCCAATTCTGG+TGG | 0.572089 | 1.4:-50274908 | MS.gene72416:CDS |
| TATGACCACAAAGATGAGAA+TGG | 0.579877 | 1.4:-50274487 | MS.gene72416:CDS |
| GGTAGACTTGCAGCAAAACA+AGG | 0.581972 | 1.4:-50274529 | MS.gene72416:CDS |
| TCACCATTTGCAAGTCGTGT+CGG | 0.595307 | 1.4:+50273582 | None:intergenic |
| GACTTGCAGCAAAACAAGGA+TGG | 0.607232 | 1.4:-50274525 | MS.gene72416:CDS |
| TTCTGATACCCAAGTGAGGG+AGG | 0.616633 | 1.4:+50273887 | None:intergenic |
| TCATCATACCTCCCTCACTT+GGG | 0.617977 | 1.4:-50273895 | MS.gene72416:CDS |
| TGAACCAACAAGGAGTTCCA+AGG | 0.619826 | 1.4:+50274877 | None:intergenic |
| ACACGACTTGCAAATGGTGA+AGG | 0.624081 | 1.4:-50273579 | MS.gene72416:CDS |
| CCTTAACACCACCAGAATTG+GGG | 0.629737 | 1.4:+50274900 | None:intergenic |
| TATACCTGGTCTCCATCTTG+AGG | 0.633736 | 1.4:+50274845 | None:intergenic |
| ATATTGAACTGGTTGGTCTG+AGG | 0.637937 | 1.4:-50273530 | MS.gene72416:CDS |
| TGGGTATCAGAACACATCAC+AGG | 0.647520 | 1.4:-50273876 | MS.gene72416:CDS |
| GAACTTCACCTGAACCAACA+AGG | 0.650571 | 1.4:+50274867 | None:intergenic |
| AGTTCCTCAAGATGGAGACC+AGG | 0.651081 | 1.4:-50274849 | MS.gene72416:intron |
| GTGAGATCAATGAAAGTCGG+TGG | 0.665800 | 1.4:-50273929 | MS.gene72416:CDS |
| AGCTTACCTTACCAGAACCA+AGG | 0.667313 | 1.4:+50274440 | None:intergenic |
| GGTAATTGCTGGAATTGATG+TGG | 0.679818 | 1.4:-50273954 | MS.gene72416:CDS |
| GTGTTCTGATACCCAAGTGA+GGG | 0.691969 | 1.4:+50273884 | None:intergenic |
| GCAGTGAGATCAATGAAAGT+CGG | 0.693946 | 1.4:-50273932 | MS.gene72416:CDS |
| TGTGTTCTGATACCCAAGTG+AGG | 0.735364 | 1.4:+50273883 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTGTTTTATTTTATTTTGT+TGG | - | chr1.4:50273926-50273945 | MS.gene72416:CDS | 10.0% |
| !! | AAAAATTTCAGACTTATATA+TGG | - | chr1.4:50274568-50274587 | MS.gene72416:intron | 15.0% |
| !! | AAATTCAGAATTTATAGATA+TGG | - | chr1.4:50273886-50273905 | MS.gene72416:CDS | 15.0% |
| !! | AAATTCTGAATTTAGCATAT+TGG | + | chr1.4:50273879-50273898 | None:intergenic | 20.0% |
| !! | AATGCTAATAGACAAATTAA+TGG | - | chr1.4:50273989-50274008 | MS.gene72416:intron | 20.0% |
| !! | ACTTATATTTGATATTGAAC+TGG | - | chr1.4:50275101-50275120 | MS.gene72416:CDS | 20.0% |
| !! | ATAAAAGGATTTCTTACATT+AGG | + | chr1.4:50274803-50274822 | None:intergenic | 20.0% |
| !! | ATAATAAAATACTGTGTCTT+TGG | - | chr1.4:50273533-50273552 | MS.gene72416:CDS | 20.0% |
| !! | TTGATTATAACCAGTAAATT+TGG | - | chr1.4:50274224-50274243 | MS.gene72416:intron | 20.0% |
| !!! | ACAAACTTATTTTGATCATT+TGG | + | chr1.4:50274392-50274411 | None:intergenic | 20.0% |
| !!! | ATTGGTTTCTAGATATATAT+AGG | + | chr1.4:50273861-50273880 | None:intergenic | 20.0% |
| !!! | CAAACTTATTTTGATCATTT+GGG | + | chr1.4:50274391-50274410 | None:intergenic | 20.0% |
| !!! | GTAGGTTTGTTTTTTATTTA+GGG | - | chr1.4:50273965-50273984 | MS.gene72416:intron | 20.0% |
| !!! | TAATAGACAAATTAATGGAT+AGG | - | chr1.4:50273994-50274013 | MS.gene72416:intron | 20.0% |
| !!! | TGTAGGTTTGTTTTTTATTT+AGG | - | chr1.4:50273964-50273983 | MS.gene72416:intron | 20.0% |
| ! | AAACAAAAAAGTTCTTTGAC+AGG | - | chr1.4:50275010-50275029 | MS.gene72416:intron | 25.0% |
| ! | AGATATATATAGGAGATTTG+AGG | + | chr1.4:50273851-50273870 | None:intergenic | 25.0% |
| ! | AGTAAATGTTGTTACATTGA+AGG | - | chr1.4:50274073-50274092 | MS.gene72416:intron | 25.0% |
| ! | ATATTTGATATTGAACTGGT+TGG | - | chr1.4:50275105-50275124 | MS.gene72416:CDS | 25.0% |
| ! | ATGTAATTCTGGATCATAAA+AGG | + | chr1.4:50274818-50274837 | None:intergenic | 25.0% |
| ! | TATAACCAGTAAATTTGGTT+TGG | - | chr1.4:50274229-50274248 | MS.gene72416:intron | 25.0% |
| !! | TGAAATTTTTACTGTCCTTA+TGG | + | chr1.4:50274558-50274577 | None:intergenic | 25.0% |
| !!! | CATACTAAGTTTTTTCCATA+AGG | - | chr1.4:50274540-50274559 | MS.gene72416:CDS | 25.0% |
| AAACACCAAACCAAATTTAC+TGG | + | chr1.4:50274237-50274256 | None:intergenic | 30.0% | |
| AAAGGATTTCTTACATTAGG+CGG | + | chr1.4:50274800-50274819 | None:intergenic | 30.0% | |
| AAGGTTGAGATTCATTACTA+TGG | - | chr1.4:50274092-50274111 | MS.gene72416:intron | 30.0% | |
| ATCATGTTGCATACTAAGAA+AGG | - | chr1.4:50274632-50274651 | MS.gene72416:intron | 30.0% | |
| CAAACGTAACTATGTAATTC+TGG | + | chr1.4:50274829-50274848 | None:intergenic | 30.0% | |
| CAGACTTATATATGGATCTT+TGG | - | chr1.4:50274576-50274595 | MS.gene72416:intron | 30.0% | |
| CTTAGTATGCAACATGATAA+AGG | + | chr1.4:50274630-50274649 | None:intergenic | 30.0% | |
| GAGATTAAAGACAGAGATAA+GGG | + | chr1.4:50273623-50273642 | None:intergenic | 30.0% | |
| ! | AGCAATTTTAAGCATCAATG+TGG | - | chr1.4:50274484-50274503 | MS.gene72416:CDS | 30.0% |
| ! | GACAAATTAATGGATAGGTA+AGG | - | chr1.4:50273999-50274018 | MS.gene72416:intron | 30.0% |
| ! | TAAAATTGCTTCTTGAGTTG+TGG | + | chr1.4:50274474-50274493 | None:intergenic | 30.0% |
| ! | TACATGGATATTTTGTGTGA+GGG | + | chr1.4:50273586-50273605 | None:intergenic | 30.0% |
| ! | TTACATGGATATTTTGTGTG+AGG | + | chr1.4:50273587-50273606 | None:intergenic | 30.0% |
| AAGGATTTCTTACATTAGGC+GGG | + | chr1.4:50274799-50274818 | None:intergenic | 35.0% | |
| AAGTCGTGTCGGATTAAAAA+TGG | + | chr1.4:50275052-50275071 | None:intergenic | 35.0% | |
| AGGACAAAGACAAAAGGATT+AGG | + | chr1.4:50274185-50274204 | None:intergenic | 35.0% | |
| AGGAGTTAATCTTGTGAGAA+TGG | - | chr1.4:50274652-50274671 | MS.gene72416:intron | 35.0% | |
| AGTTCAGAGAAATCTGCTAT+TGG | + | chr1.4:50273722-50273741 | None:intergenic | 35.0% | |
| ATTCTCATCTTTGTGGTCAT+AGG | + | chr1.4:50274156-50274175 | None:intergenic | 35.0% | |
| GATTAAAGCAGAGAAAGTAG+TGG | + | chr1.4:50273645-50273664 | None:intergenic | 35.0% | |
| GGAGATTAAAGACAGAGATA+AGG | + | chr1.4:50273624-50273643 | None:intergenic | 35.0% | |
| GGTAGAGTATGAGAATTTGT+AGG | - | chr1.4:50273947-50273966 | MS.gene72416:CDS | 35.0% | |
| TATGACCACAAAGATGAGAA+TGG | - | chr1.4:50274155-50274174 | MS.gene72416:intron | 35.0% | |
| TTAAATGAGAGAGCTATACC+TGG | + | chr1.4:50273814-50273833 | None:intergenic | 35.0% | |
| ! | AATCCTTTTGTCTTTGTCCT+TGG | - | chr1.4:50274185-50274204 | MS.gene72416:intron | 35.0% |
| ! | ATGCAACATGATAAAGGCAT+AGG | + | chr1.4:50274624-50274643 | None:intergenic | 35.0% |
| ! | GGTCTTCTTGTTATGTTACA+TGG | + | chr1.4:50273602-50273621 | None:intergenic | 35.0% |
| AAAAGTTCTTTGACAGGCAG+AGG | - | chr1.4:50275016-50275035 | MS.gene72416:intron | 40.0% | |
| AATGGTGAAGGTTCGACTTT+AGG | - | chr1.4:50275075-50275094 | MS.gene72416:CDS | 40.0% | |
| AGAAGGTGAAGGAGAAGAAT+AGG | + | chr1.4:50273690-50273709 | None:intergenic | 40.0% | |
| ATGGTGAAGGTTCGACTTTA+GGG | - | chr1.4:50275076-50275095 | MS.gene72416:CDS | 40.0% | |
| GAACCAAGGACAAAGACAAA+AGG | + | chr1.4:50274191-50274210 | None:intergenic | 40.0% | |
| GATCACCATTCTCATCTTTG+TGG | + | chr1.4:50274163-50274182 | None:intergenic | 40.0% | |
| GCAGTGAGATCAATGAAAGT+CGG | - | chr1.4:50274710-50274729 | MS.gene72416:intron | 40.0% | |
| GGTAATTGCTGGAATTGATG+TGG | - | chr1.4:50274688-50274707 | MS.gene72416:intron | 40.0% | |
| TTTCTTACATTAGGCGGGAT+AGG | + | chr1.4:50274794-50274813 | None:intergenic | 40.0% | |
| TTTGTCTTTGTCCTTGGTTC+TGG | - | chr1.4:50274191-50274210 | MS.gene72416:intron | 40.0% | |
| ! | ATATTTTGTGTGAGGGAGAG+TGG | + | chr1.4:50273579-50273598 | None:intergenic | 40.0% |
| !! | ATATTGAACTGGTTGGTCTG+AGG | - | chr1.4:50275112-50275131 | MS.gene72416:CDS | 40.0% |
| !!! | GCTATTGGACGTTTTGAAGA+AGG | + | chr1.4:50273707-50273726 | None:intergenic | 40.0% |
| AATCCGACACGACTTGCAAA+TGG | - | chr1.4:50275057-50275076 | MS.gene72416:intron | 45.0% | |
| ACACGACTTGCAAATGGTGA+AGG | - | chr1.4:50275063-50275082 | MS.gene72416:CDS | 45.0% | |
| AGCTTACCTTACCAGAACCA+AGG | + | chr1.4:50274205-50274224 | None:intergenic | 45.0% | |
| CCTTAACACCACCAGAATTG+GGG | + | chr1.4:50273745-50273764 | None:intergenic | 45.0% | |
| GAACTTCACCTGAACCAACA+AGG | + | chr1.4:50273778-50273797 | None:intergenic | 45.0% | |
| GAAGAATAGGAAGGTGGAAG+AGG | + | chr1.4:50273677-50273696 | None:intergenic | 45.0% | |
| GAAGGAGAAGAATAGGAAGG+TGG | + | chr1.4:50273683-50273702 | None:intergenic | 45.0% | |
| GACTTGCAGCAAAACAAGGA+TGG | - | chr1.4:50274117-50274136 | MS.gene72416:intron | 45.0% | |
| GCCTTAACACCACCAGAATT+GGG | + | chr1.4:50273746-50273765 | None:intergenic | 45.0% | |
| GGTAGACTTGCAGCAAAACA+AGG | - | chr1.4:50274113-50274132 | MS.gene72416:intron | 45.0% | |
| GGTGAAGGAGAAGAATAGGA+AGG | + | chr1.4:50273686-50273705 | None:intergenic | 45.0% | |
| GTGAGATCAATGAAAGTCGG+TGG | - | chr1.4:50274713-50274732 | MS.gene72416:intron | 45.0% | |
| GTGTTCTGATACCCAAGTGA+GGG | + | chr1.4:50274761-50274780 | None:intergenic | 45.0% | |
| TATACCTGGTCTCCATCTTG+AGG | + | chr1.4:50273800-50273819 | None:intergenic | 45.0% | |
| TCACCATTTGCAAGTCGTGT+CGG | + | chr1.4:50275063-50275082 | None:intergenic | 45.0% | |
| TCAGGTGAAGTTCCTCAAGA+TGG | - | chr1.4:50273785-50273804 | MS.gene72416:intron | 45.0% | |
| TCATCATACCTCCCTCACTT+GGG | - | chr1.4:50274747-50274766 | MS.gene72416:intron | 45.0% | |
| TGAACCAACAAGGAGTTCCA+AGG | + | chr1.4:50273768-50273787 | None:intergenic | 45.0% | |
| TGCTGTGTTCAGGTAATTGC+TGG | - | chr1.4:50274677-50274696 | MS.gene72416:intron | 45.0% | |
| TGGGTATCAGAACACATCAC+AGG | - | chr1.4:50274766-50274785 | MS.gene72416:intron | 45.0% | |
| TGTGTTCTGATACCCAAGTG+AGG | + | chr1.4:50274762-50274781 | None:intergenic | 45.0% | |
| TTCTCTGAACTCCCCAATTC+TGG | - | chr1.4:50273731-50273750 | MS.gene72416:intron | 45.0% | |
| !! | CTTTGTCCTTGGTTCTGGTA+AGG | - | chr1.4:50274196-50274215 | MS.gene72416:intron | 45.0% |
| !! | GAGAATGGATTGCTGTGTTC+AGG | - | chr1.4:50274667-50274686 | MS.gene72416:intron | 45.0% |
| !! | TTGGAACTCCTTGTTGGTTC+AGG | - | chr1.4:50273767-50273786 | MS.gene72416:intron | 45.0% |
| !!! | GGACGTTTTGAAGAAGGTGA+AGG | + | chr1.4:50273701-50273720 | None:intergenic | 45.0% |
| AAGGCCTTGGAACTCCTTGT+TGG | - | chr1.4:50273761-50273780 | MS.gene72416:intron | 50.0% | |
| AGTTCCTCAAGATGGAGACC+AGG | - | chr1.4:50273793-50273812 | MS.gene72416:intron | 50.0% | |
| GGCCTTAACACCACCAGAAT+TGG | + | chr1.4:50273747-50273766 | None:intergenic | 50.0% | |
| GTCATCATACCTCCCTCACT+TGG | - | chr1.4:50274746-50274765 | MS.gene72416:intron | 50.0% | |
| TCTGAACTCCCCAATTCTGG+TGG | - | chr1.4:50273734-50273753 | MS.gene72416:intron | 50.0% | |
| TTCTGATACCCAAGTGAGGG+AGG | + | chr1.4:50274758-50274777 | None:intergenic | 50.0% | |
| ! | CCCCAATTCTGGTGGTGTTA+AGG | - | chr1.4:50273742-50273761 | MS.gene72416:intron | 50.0% |
| ! | TTCTGGTGGTGTTAAGGCCT+TGG | - | chr1.4:50273748-50273767 | MS.gene72416:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 50273521 | 50275143 | 50273521 | ID=MS.gene72416 |
| chr1.4 | mRNA | 50273521 | 50275143 | 50273521 | ID=MS.gene72416.t1;Parent=MS.gene72416 |
| chr1.4 | exon | 50275062 | 50275143 | 50275062 | ID=MS.gene72416.t1.exon1;Parent=MS.gene72416.t1 |
| chr1.4 | CDS | 50275062 | 50275143 | 50275062 | ID=cds.MS.gene72416.t1;Parent=MS.gene72416.t1 |
| chr1.4 | exon | 50274850 | 50274935 | 50274850 | ID=MS.gene72416.t1.exon2;Parent=MS.gene72416.t1 |
| chr1.4 | CDS | 50274850 | 50274935 | 50274850 | ID=cds.MS.gene72416.t1;Parent=MS.gene72416.t1 |
| chr1.4 | exon | 50274447 | 50274569 | 50274447 | ID=MS.gene72416.t1.exon3;Parent=MS.gene72416.t1 |
| chr1.4 | CDS | 50274447 | 50274569 | 50274447 | ID=cds.MS.gene72416.t1;Parent=MS.gene72416.t1 |
| chr1.4 | exon | 50273859 | 50273975 | 50273859 | ID=MS.gene72416.t1.exon4;Parent=MS.gene72416.t1 |
| chr1.4 | CDS | 50273859 | 50273975 | 50273859 | ID=cds.MS.gene72416.t1;Parent=MS.gene72416.t1 |
| chr1.4 | exon | 50273521 | 50273643 | 50273521 | ID=MS.gene72416.t1.exon5;Parent=MS.gene72416.t1 |
| chr1.4 | CDS | 50273521 | 50273643 | 50273521 | ID=cds.MS.gene72416.t1;Parent=MS.gene72416.t1 |
| Gene Sequence |
| Protein sequence |
