Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33046.t1 | AES59174.2 | 92 | 337 | 18 | 1 | 1 | 328 | 1 | 337 | 9.30E-177 | 629.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33046.t1 | Q9S7X6 | 64.6 | 339 | 106 | 3 | 1 | 327 | 1 | 337 | 2.1e-124 | 446.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33046.t1 | G7I904 | 92.0 | 337 | 18 | 1 | 1 | 328 | 1 | 337 | 6.7e-177 | 629.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050133 | MS.gene33046 | 0.808775 | 2.62E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene33046 | -0.810444 | 1.15E-50 | -1.69E-46 |
| MS.gene050315 | MS.gene33046 | -0.811762 | 5.95E-51 | -1.69E-46 |
| MS.gene050710 | MS.gene33046 | 0.81182 | 5.78E-51 | -1.69E-46 |
| MS.gene050809 | MS.gene33046 | 0.80253 | 5.37E-49 | -1.69E-46 |
| MS.gene05081 | MS.gene33046 | -0.855129 | 7.76E-62 | -1.69E-46 |
| MS.gene051552 | MS.gene33046 | 0.961719 | 4.98E-120 | -1.69E-46 |
| MS.gene051838 | MS.gene33046 | -0.849332 | 3.46E-60 | -1.69E-46 |
| MS.gene051973 | MS.gene33046 | 0.806083 | 9.77E-50 | -1.69E-46 |
| MS.gene052469 | MS.gene33046 | -0.812547 | 4.01E-51 | -1.69E-46 |
| MS.gene052825 | MS.gene33046 | -0.809332 | 1.99E-50 | -1.69E-46 |
| MS.gene053097 | MS.gene33046 | 0.804781 | 1.83E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene33046 | -0.829198 | 5.89E-55 | -1.69E-46 |
| MS.gene053824 | MS.gene33046 | -0.818996 | 1.46E-52 | -1.69E-46 |
| MS.gene054107 | MS.gene33046 | -0.839962 | 1.16E-57 | -1.69E-46 |
| MS.gene054343 | MS.gene33046 | -0.819538 | 1.10E-52 | -1.69E-46 |
| MS.gene054344 | MS.gene33046 | -0.817085 | 3.96E-52 | -1.69E-46 |
| MS.gene054706 | MS.gene33046 | 0.807416 | 5.11E-50 | -1.69E-46 |
| MS.gene054742 | MS.gene33046 | 0.807885 | 4.06E-50 | -1.69E-46 |
| MS.gene054884 | MS.gene33046 | 0.821553 | 3.80E-53 | -1.69E-46 |
| MS.gene055158 | MS.gene33046 | 0.803988 | 2.68E-49 | -1.69E-46 |
| MS.gene055159 | MS.gene33046 | 0.842521 | 2.46E-58 | -1.69E-46 |
| MS.gene055577 | MS.gene33046 | -0.803824 | 2.90E-49 | -1.69E-46 |
| MS.gene056387 | MS.gene33046 | -0.807329 | 5.33E-50 | -1.69E-46 |
| MS.gene056633 | MS.gene33046 | -0.810781 | 9.72E-51 | -1.69E-46 |
| MS.gene056678 | MS.gene33046 | 0.809199 | 2.13E-50 | -1.69E-46 |
| MS.gene056680 | MS.gene33046 | 0.802972 | 4.35E-49 | -1.69E-46 |
| MS.gene056688 | MS.gene33046 | 0.80728 | 5.46E-50 | -1.69E-46 |
| MS.gene056877 | MS.gene33046 | -0.823236 | 1.54E-53 | -1.69E-46 |
| MS.gene056988 | MS.gene33046 | 0.807222 | 5.62E-50 | -1.69E-46 |
| MS.gene057081 | MS.gene33046 | 0.805436 | 1.34E-49 | -1.69E-46 |
| MS.gene057332 | MS.gene33046 | -0.822655 | 2.11E-53 | -1.69E-46 |
| MS.gene057658 | MS.gene33046 | -0.817671 | 2.92E-52 | -1.69E-46 |
| MS.gene057717 | MS.gene33046 | 0.801803 | 7.58E-49 | -1.69E-46 |
| MS.gene058076 | MS.gene33046 | 0.814055 | 1.87E-51 | -1.69E-46 |
| MS.gene058158 | MS.gene33046 | 0.845344 | 4.31E-59 | -1.69E-46 |
| MS.gene05837 | MS.gene33046 | -0.818663 | 1.74E-52 | -1.69E-46 |
| MS.gene058418 | MS.gene33046 | 0.957821 | 1.07E-115 | -1.69E-46 |
| MS.gene058444 | MS.gene33046 | -0.802749 | 4.84E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene33046 | -0.80377 | 2.97E-49 | -1.69E-46 |
| MS.gene058838 | MS.gene33046 | -0.825322 | 4.99E-54 | -1.69E-46 |
| MS.gene059023 | MS.gene33046 | -0.833441 | 5.32E-56 | -1.69E-46 |
| MS.gene05914 | MS.gene33046 | -0.834545 | 2.82E-56 | -1.69E-46 |
| MS.gene05926 | MS.gene33046 | 0.83194 | 1.26E-55 | -1.69E-46 |
| MS.gene059303 | MS.gene33046 | -0.80788 | 4.07E-50 | -1.69E-46 |
| MS.gene059512 | MS.gene33046 | -0.849622 | 2.87E-60 | -1.69E-46 |
| MS.gene05960 | MS.gene33046 | 0.827965 | 1.17E-54 | -1.69E-46 |
| MS.gene059891 | MS.gene33046 | 0.810714 | 1.00E-50 | -1.69E-46 |
| MS.gene060087 | MS.gene33046 | 0.810796 | 9.65E-51 | -1.69E-46 |
| MS.gene06092 | MS.gene33046 | -0.811349 | 7.32E-51 | -1.69E-46 |
| MS.gene061231 | MS.gene33046 | -0.801917 | 7.18E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33046.t1 | MTR_1g015300 | 91.988 | 337 | 18 | 1 | 1 | 328 | 1 | 337 | 0.0 | 634 |
| MS.gene33046.t1 | MTR_3g104960 | 68.935 | 338 | 85 | 4 | 1 | 327 | 1 | 329 | 7.94e-169 | 473 |
| MS.gene33046.t1 | MTR_5g006140 | 63.787 | 301 | 100 | 1 | 32 | 323 | 25 | 325 | 3.76e-139 | 397 |
| MS.gene33046.t1 | MTR_5g006140 | 72.093 | 215 | 60 | 0 | 32 | 246 | 25 | 239 | 5.09e-117 | 338 |
| MS.gene33046.t1 | MTR_5g034470 | 34.156 | 243 | 143 | 8 | 11 | 246 | 33 | 265 | 8.08e-37 | 135 |
| MS.gene33046.t1 | MTR_5g034470 | 34.156 | 243 | 143 | 8 | 11 | 246 | 33 | 265 | 9.57e-37 | 135 |
| MS.gene33046.t1 | MTR_8g063940 | 31.507 | 219 | 136 | 6 | 34 | 246 | 54 | 264 | 6.27e-32 | 122 |
| MS.gene33046.t1 | MTR_3g078190 | 33.103 | 145 | 96 | 1 | 47 | 190 | 59 | 203 | 1.17e-21 | 94.0 |
| MS.gene33046.t1 | MTR_3g091090 | 31.188 | 202 | 133 | 4 | 46 | 246 | 83 | 279 | 8.48e-21 | 92.0 |
| MS.gene33046.t1 | MTR_1g028360 | 33.333 | 186 | 120 | 3 | 46 | 230 | 88 | 270 | 5.70e-19 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33046.t1 | AT5G19530 | 64.602 | 339 | 106 | 3 | 1 | 327 | 1 | 337 | 2.68e-159 | 449 |
| MS.gene33046.t1 | AT5G19530 | 65.846 | 325 | 100 | 2 | 12 | 327 | 8 | 330 | 1.85e-156 | 441 |
| MS.gene33046.t1 | AT1G23820 | 34.158 | 202 | 127 | 4 | 46 | 246 | 105 | 301 | 2.24e-35 | 131 |
| MS.gene33046.t1 | AT1G23820 | 34.158 | 202 | 127 | 4 | 46 | 246 | 61 | 257 | 3.47e-35 | 130 |
| MS.gene33046.t1 | AT1G70310 | 32.420 | 219 | 134 | 6 | 34 | 246 | 51 | 261 | 1.37e-33 | 126 |
| MS.gene33046.t1 | AT5G53120 | 30.469 | 256 | 156 | 6 | 46 | 291 | 84 | 327 | 1.68e-21 | 94.0 |
| MS.gene33046.t1 | AT5G53120 | 30.469 | 256 | 156 | 6 | 46 | 291 | 84 | 327 | 1.68e-21 | 94.0 |
| MS.gene33046.t1 | AT5G53120 | 30.469 | 256 | 156 | 6 | 46 | 291 | 84 | 327 | 1.68e-21 | 94.0 |
| MS.gene33046.t1 | AT5G53120 | 30.469 | 256 | 156 | 6 | 46 | 291 | 84 | 327 | 1.68e-21 | 94.0 |
| MS.gene33046.t1 | AT5G53120 | 30.469 | 256 | 156 | 6 | 46 | 291 | 84 | 327 | 1.68e-21 | 94.0 |
| MS.gene33046.t1 | AT5G53120 | 31.754 | 211 | 138 | 4 | 46 | 255 | 101 | 306 | 2.04e-21 | 94.0 |
| MS.gene33046.t1 | AT5G53120 | 27.562 | 283 | 156 | 8 | 46 | 291 | 84 | 354 | 7.36e-18 | 84.0 |
Find 69 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGAACCTGTTGAGTTTAA+AGG | 0.204551 | 1.1:-6547668 | None:intergenic |
| TCATTAACGTAAAACATTTC+TGG | 0.211926 | 1.1:-6547544 | None:intergenic |
| AAAATACCTGGTTGCAAATA+AGG | 0.242099 | 1.1:+6557877 | MS.gene33046:CDS |
| ACATGGTTTGGTTGAACTTC+AGG | 0.282517 | 1.1:-6547583 | None:intergenic |
| AGAATTGAAGCAAGAATAAA+AGG | 0.285461 | 1.1:+6559104 | MS.gene33046:CDS |
| TCCACAGCATCAATCAATAT+TGG | 0.287518 | 1.1:-6559071 | None:intergenic |
| CCCCAAGACGGTGTTTATAA+TGG | 0.300655 | 1.1:+6557003 | MS.gene33046:CDS |
| CCCATTATAAACACCGTCTT+GGG | 0.300710 | 1.1:-6557004 | None:intergenic |
| CTCTTGGATACAAAGCGTTT+TGG | 0.309876 | 1.1:+6547953 | MS.gene33046:CDS |
| TGATATCATTGTTGGAGATT+TGG | 0.339425 | 1.1:+6558113 | MS.gene33046:CDS |
| CTTCACAACTTTCTCATAGA+AGG | 0.351490 | 1.1:-6558181 | None:intergenic |
| ATGCCATTGTCAGTGAGTTT+AGG | 0.356612 | 1.1:-6558204 | None:intergenic |
| GAGAAGTTTGATATCATTGT+TGG | 0.364473 | 1.1:+6558105 | MS.gene33046:CDS |
| TTCTTATAGGCTGGACCAGC+AGG | 0.390978 | 1.1:+6558499 | MS.gene33046:intron |
| AATGGTCCTTTAAACTCAAC+AGG | 0.400428 | 1.1:+6547662 | MS.gene33046:CDS |
| ATACCAGGACATTGCTCTCT+TGG | 0.416640 | 1.1:+6547937 | MS.gene33046:CDS |
| TGCTCTATCTAGACGGTGCT+TGG | 0.419252 | 1.1:+6559132 | MS.gene33046:CDS |
| CCCAAGACGGTGTTTATAAT+GGG | 0.420987 | 1.1:+6557004 | MS.gene33046:CDS |
| ATTGATTGATGCTGTGGAAA+TGG | 0.421971 | 1.1:+6559076 | MS.gene33046:CDS |
| GATAATGCTAGATTTATTCC+TGG | 0.440598 | 1.1:+6559321 | MS.gene33046:CDS |
| CATGGTTTGGTTGAACTTCA+GGG | 0.443362 | 1.1:-6547582 | None:intergenic |
| TGATTCTTCCACATGGTATG+AGG | 0.443438 | 1.1:+6547618 | MS.gene33046:CDS |
| TATACACAACACCATAAAAC+AGG | 0.448583 | 1.1:+6558552 | MS.gene33046:CDS |
| TCAACGTTGTTGACATGGTT+TGG | 0.458692 | 1.1:-6547595 | None:intergenic |
| AATATTCCTGCAGGAGGTGG+TGG | 0.466008 | 1.1:+6557844 | MS.gene33046:intron |
| ACCTACTAGTAAAAGATTTG+AGG | 0.468209 | 1.1:-6558599 | None:intergenic |
| ATATAGGCGTTGATGCTTGA+TGG | 0.475521 | 1.1:+6556106 | MS.gene33046:intron |
| TGGTGAAGGTTCAGCAGCTA+GGG | 0.487536 | 1.1:+6557030 | MS.gene33046:CDS |
| TCCCATTATAAACACCGTCT+TGG | 0.488858 | 1.1:-6557005 | None:intergenic |
| CATTTCTGGTGCCTCTCCCA+TGG | 0.496543 | 1.1:-6547530 | None:intergenic |
| TTTATAATGGGAGGTGGTGA+AGG | 0.498635 | 1.1:+6557016 | MS.gene33046:CDS |
| TATTCATGATGGTAGATGAA+TGG | 0.501719 | 1.1:-6559157 | None:intergenic |
| GGCTGAATTAGAGAAAAGAA+AGG | 0.510493 | 1.1:+6558083 | MS.gene33046:CDS |
| GCTAGATTTATTCCTGGTCA+TGG | 0.513206 | 1.1:+6559327 | MS.gene33046:CDS |
| TTGGCTGACCCACTTGAAGA+TGG | 0.518779 | 1.1:+6558132 | MS.gene33046:CDS |
| AGCTCTCAAGCACAAGTCAA+TGG | 0.518812 | 1.1:+6557054 | MS.gene33046:CDS |
| TCATTGATGATGATCTCAAA+TGG | 0.521281 | 1.1:+6547644 | MS.gene33046:CDS |
| GCATAGCATGTCGTCCCTAA+AGG | 0.523854 | 1.1:+6559349 | MS.gene33046:CDS |
| GTGGTGAAGGTTCAGCAGCT+AGG | 0.528642 | 1.1:+6557029 | MS.gene33046:CDS |
| AATCATCAACGTTGTTGACA+TGG | 0.529958 | 1.1:-6547600 | None:intergenic |
| ACCAATATTGATTGATGCTG+TGG | 0.532194 | 1.1:+6559070 | MS.gene33046:CDS |
| AACAAATAAAACCATGGGAG+AGG | 0.560741 | 1.1:+6547519 | None:intergenic |
| CGACATGCTATGCCATGACC+AGG | 0.567260 | 1.1:-6559339 | None:intergenic |
| AACTTTCTCATAGAAGGACT+TGG | 0.568835 | 1.1:-6558175 | None:intergenic |
| TATCCAAGAGAGCAATGTCC+TGG | 0.569197 | 1.1:-6547940 | None:intergenic |
| TTACGTTAATGAGTTTGCAA+AGG | 0.578372 | 1.1:+6547555 | MS.gene33046:CDS |
| TTGTGTGAAAAGATACCTGC+TGG | 0.579877 | 1.1:-6558514 | None:intergenic |
| GAACACGGTCTTATTCATGA+TGG | 0.581081 | 1.1:-6559168 | None:intergenic |
| ATGCCTCCTTATTTGCAACC+AGG | 0.583465 | 1.1:-6557883 | None:intergenic |
| CAGCATCAATCAATATTGGT+TGG | 0.586960 | 1.1:-6559067 | None:intergenic |
| TTTGGATGACATAAAAGAGA+GGG | 0.601046 | 1.1:-6556184 | None:intergenic |
| GGATTTCTGCAGAAAATACC+TGG | 0.607431 | 1.1:+6557865 | MS.gene33046:CDS |
| GGAAAATTGCTCTATCTAGA+CGG | 0.609264 | 1.1:+6559125 | MS.gene33046:CDS |
| AAGCCTAAACTCACTGACAA+TGG | 0.615534 | 1.1:+6558201 | MS.gene33046:CDS |
| CCATTATAAACACCGTCTTG+GGG | 0.615981 | 1.1:-6557003 | None:intergenic |
| TAAAGCGATTAGTGAATACC+AGG | 0.616434 | 1.1:+6547922 | MS.gene33046:CDS |
| ACGGTGTTTATAATGGGAGG+TGG | 0.620093 | 1.1:+6557010 | MS.gene33046:CDS |
| TTGGTGTAGAGCTGATAGCA+AGG | 0.624084 | 1.1:-6558156 | None:intergenic |
| ACTTTGCACAGTTGATGACA+AGG | 0.637400 | 1.1:-6557925 | None:intergenic |
| TAGCAAGGACCATCTTCAAG+TGG | 0.638054 | 1.1:-6558141 | None:intergenic |
| AGCAAGGACCATCTTCAAGT+GGG | 0.640286 | 1.1:-6558140 | None:intergenic |
| CTTTGGATGACATAAAAGAG+AGG | 0.645177 | 1.1:-6556185 | None:intergenic |
| GTACACTTACGATTTGAACA+CGG | 0.645888 | 1.1:-6559183 | None:intergenic |
| ATACCTGGTTGCAAATAAGG+AGG | 0.651779 | 1.1:+6557880 | MS.gene33046:CDS |
| AGTTATGTGCGATATTGACA+AGG | 0.654516 | 1.1:+6557084 | MS.gene33046:CDS |
| ATATATTGGCAGCCCCAAGA+CGG | 0.656927 | 1.1:+6556991 | MS.gene33046:intron |
| AAGACGGTGTTTATAATGGG+AGG | 0.658751 | 1.1:+6557007 | MS.gene33046:CDS |
| AATGATTTCCTCATACCATG+TGG | 0.685776 | 1.1:-6547626 | None:intergenic |
| ACGTTGATGATTCTTCCACA+TGG | 0.698888 | 1.1:+6547611 | MS.gene33046:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 6547532 | 6559368 | 6547532 | ID=MS.gene33046 |
| chr1.1 | mRNA | 6547532 | 6559368 | 6547532 | ID=MS.gene33046.t1;Parent=MS.gene33046 |
| chr1.1 | exon | 6547532 | 6547683 | 6547532 | ID=MS.gene33046.t1.exon1;Parent=MS.gene33046.t1 |
| chr1.1 | CDS | 6547532 | 6547683 | 6547532 | ID=cds.MS.gene33046.t1;Parent=MS.gene33046.t1 |
| chr1.1 | exon | 6547913 | 6547979 | 6547913 | ID=MS.gene33046.t1.exon2;Parent=MS.gene33046.t1 |
| chr1.1 | CDS | 6547913 | 6547979 | 6547913 | ID=cds.MS.gene33046.t1;Parent=MS.gene33046.t1 |
| chr1.1 | exon | 6556112 | 6556206 | 6556112 | ID=MS.gene33046.t1.exon3;Parent=MS.gene33046.t1 |
| chr1.1 | CDS | 6556112 | 6556206 | 6556112 | ID=cds.MS.gene33046.t1;Parent=MS.gene33046.t1 |
| chr1.1 | exon | 6557003 | 6557105 | 6557003 | ID=MS.gene33046.t1.exon4;Parent=MS.gene33046.t1 |
| chr1.1 | CDS | 6557003 | 6557105 | 6557003 | ID=cds.MS.gene33046.t1;Parent=MS.gene33046.t1 |
| chr1.1 | exon | 6557857 | 6557945 | 6557857 | ID=MS.gene33046.t1.exon5;Parent=MS.gene33046.t1 |
| chr1.1 | CDS | 6557857 | 6557945 | 6557857 | ID=cds.MS.gene33046.t1;Parent=MS.gene33046.t1 |
| chr1.1 | exon | 6558083 | 6558239 | 6558083 | ID=MS.gene33046.t1.exon6;Parent=MS.gene33046.t1 |
| chr1.1 | CDS | 6558083 | 6558239 | 6558083 | ID=cds.MS.gene33046.t1;Parent=MS.gene33046.t1 |
| chr1.1 | exon | 6558508 | 6558615 | 6558508 | ID=MS.gene33046.t1.exon7;Parent=MS.gene33046.t1 |
| chr1.1 | CDS | 6558508 | 6558615 | 6558508 | ID=cds.MS.gene33046.t1;Parent=MS.gene33046.t1 |
| chr1.1 | exon | 6559059 | 6559195 | 6559059 | ID=MS.gene33046.t1.exon8;Parent=MS.gene33046.t1 |
| chr1.1 | CDS | 6559059 | 6559195 | 6559059 | ID=cds.MS.gene33046.t1;Parent=MS.gene33046.t1 |
| chr1.1 | exon | 6559290 | 6559368 | 6559290 | ID=MS.gene33046.t1.exon9;Parent=MS.gene33046.t1 |
| chr1.1 | CDS | 6559290 | 6559368 | 6559290 | ID=cds.MS.gene33046.t1;Parent=MS.gene33046.t1 |
| Gene Sequence |
| Protein sequence |
