Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82269.t1 | XP_013453015.1 | 90.9 | 231 | 17 | 2 | 1 | 227 | 1 | 231 | 3.90E-97 | 364.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82269.t1 | A0A072UD80 | 90.9 | 231 | 17 | 2 | 1 | 227 | 1 | 231 | 2.8e-97 | 364.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene82269 | 0.81358 | 2.38E-51 | -1.69E-46 |
| MS.gene04920 | MS.gene82269 | 0.815234 | 1.03E-51 | -1.69E-46 |
| MS.gene049254 | MS.gene82269 | 0.801359 | 9.35E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene82269 | 0.826806 | 2.22E-54 | -1.69E-46 |
| MS.gene049419 | MS.gene82269 | -0.811182 | 7.96E-51 | -1.69E-46 |
| MS.gene049499 | MS.gene82269 | 0.813979 | 1.95E-51 | -1.69E-46 |
| MS.gene049652 | MS.gene82269 | 0.809752 | 1.62E-50 | -1.69E-46 |
| MS.gene049680 | MS.gene82269 | 0.823285 | 1.50E-53 | -1.69E-46 |
| MS.gene050571 | MS.gene82269 | 0.827631 | 1.41E-54 | -1.69E-46 |
| MS.gene050752 | MS.gene82269 | 0.806474 | 8.08E-50 | -1.69E-46 |
| MS.gene050955 | MS.gene82269 | 0.81311 | 3.02E-51 | -1.69E-46 |
| MS.gene051332 | MS.gene82269 | 0.802958 | 4.38E-49 | -1.69E-46 |
| MS.gene051343 | MS.gene82269 | 0.829031 | 6.46E-55 | -1.69E-46 |
| MS.gene051597 | MS.gene82269 | 0.812405 | 4.31E-51 | -1.69E-46 |
| MS.gene051676 | MS.gene82269 | 0.839309 | 1.71E-57 | -1.69E-46 |
| MS.gene051699 | MS.gene82269 | 0.813117 | 3.01E-51 | -1.69E-46 |
| MS.gene051831 | MS.gene82269 | 0.800044 | 1.73E-48 | -1.69E-46 |
| MS.gene051832 | MS.gene82269 | 0.816669 | 4.91E-52 | -1.69E-46 |
| MS.gene051848 | MS.gene82269 | -0.80398 | 2.69E-49 | -1.69E-46 |
| MS.gene05188 | MS.gene82269 | 0.819376 | 1.20E-52 | -1.69E-46 |
| MS.gene051975 | MS.gene82269 | 0.88132 | 2.65E-70 | -1.69E-46 |
| MS.gene052889 | MS.gene82269 | 0.805054 | 1.61E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene82269 | 0.806735 | 7.12E-50 | -1.69E-46 |
| MS.gene053006 | MS.gene82269 | 0.800195 | 1.61E-48 | -1.69E-46 |
| MS.gene053094 | MS.gene82269 | 0.848849 | 4.71E-60 | -1.69E-46 |
| MS.gene053298 | MS.gene82269 | 0.812472 | 4.17E-51 | -1.69E-46 |
| MS.gene053340 | MS.gene82269 | 0.836768 | 7.71E-57 | -1.69E-46 |
| MS.gene053564 | MS.gene82269 | 0.820244 | 7.60E-53 | -1.69E-46 |
| MS.gene053963 | MS.gene82269 | 0.836325 | 1.00E-56 | -1.69E-46 |
| MS.gene054222 | MS.gene82269 | 0.809211 | 2.12E-50 | -1.69E-46 |
| MS.gene054403 | MS.gene82269 | 0.810641 | 1.04E-50 | -1.69E-46 |
| MS.gene054576 | MS.gene82269 | 0.808508 | 2.99E-50 | -1.69E-46 |
| MS.gene05469 | MS.gene82269 | 0.865116 | 7.46E-65 | -1.69E-46 |
| MS.gene054892 | MS.gene82269 | 0.811923 | 5.49E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene82269 | 0.808611 | 2.84E-50 | -1.69E-46 |
| MS.gene055147 | MS.gene82269 | 0.817919 | 2.57E-52 | -1.69E-46 |
| MS.gene055148 | MS.gene82269 | 0.806027 | 1.00E-49 | -1.69E-46 |
| MS.gene055313 | MS.gene82269 | 0.808461 | 3.06E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene82269 | 0.804608 | 1.99E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene82269 | 0.828493 | 8.72E-55 | -1.69E-46 |
| MS.gene056986 | MS.gene82269 | 0.876216 | 1.67E-68 | -1.69E-46 |
| MS.gene057102 | MS.gene82269 | 0.833257 | 5.92E-56 | -1.69E-46 |
| MS.gene057208 | MS.gene82269 | 0.83795 | 3.84E-57 | -1.69E-46 |
| MS.gene057890 | MS.gene82269 | -0.819481 | 1.14E-52 | -1.69E-46 |
| MS.gene057893 | MS.gene82269 | -0.801165 | 1.02E-48 | -1.69E-46 |
| MS.gene057999 | MS.gene82269 | 0.821925 | 3.12E-53 | -1.69E-46 |
| MS.gene058279 | MS.gene82269 | 0.83749 | 5.04E-57 | -1.69E-46 |
| MS.gene058592 | MS.gene82269 | 0.826116 | 3.24E-54 | -1.69E-46 |
| MS.gene058708 | MS.gene82269 | -0.801853 | 7.40E-49 | -1.69E-46 |
| MS.gene059051 | MS.gene82269 | 0.820457 | 6.79E-53 | -1.69E-46 |
| MS.gene059146 | MS.gene82269 | 0.815714 | 8.03E-52 | -1.69E-46 |
| MS.gene05952 | MS.gene82269 | 0.810927 | 9.04E-51 | -1.69E-46 |
| MS.gene05985 | MS.gene82269 | -0.851512 | 8.46E-61 | -1.69E-46 |
| MS.gene059901 | MS.gene82269 | -0.823674 | 1.22E-53 | -1.69E-46 |
| MS.gene059935 | MS.gene82269 | 0.807278 | 5.46E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene82269 | 0.835439 | 1.68E-56 | -1.69E-46 |
| MS.gene060244 | MS.gene82269 | 0.825667 | 4.14E-54 | -1.69E-46 |
| MS.gene060365 | MS.gene82269 | 0.861973 | 7.04E-64 | -1.69E-46 |
| MS.gene060527 | MS.gene82269 | 0.812197 | 4.79E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene82269 | 0.820718 | 5.92E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene82269 | 0.825315 | 5.01E-54 | -1.69E-46 |
| MS.gene061100 | MS.gene82269 | 0.806521 | 7.90E-50 | -1.69E-46 |
| MS.gene061188 | MS.gene82269 | 0.844137 | 9.11E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82269.t1 | MTR_6g084600 | 92.641 | 231 | 13 | 2 | 1 | 227 | 1 | 231 | 3.18e-151 | 419 |
| MS.gene82269.t1 | MTR_8g104140 | 39.474 | 228 | 86 | 10 | 5 | 227 | 6 | 186 | 7.69e-27 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82269.t1 | AT1G13360 | 31.905 | 210 | 87 | 9 | 49 | 226 | 8 | 193 | 7.84e-12 | 62.4 |
Find 0 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATGAGTTAACATCAGAAA+AGG | - | 42700:15901-15920 | None:intergenic | 30.0% | |
| ATAACCAAAGTCATCAAAAC+CGG | - | 42700:16333-16352 | None:intergenic | 30.0% | |
| ! | AGTTGATACTAAGATTCGTA+GGG | + | 42700:15869-15888 | MS.gene82269:CDS | 30.0% |
| ATCAACTCTCTTTCTCTTGT+CGG | - | 42700:15814-15833 | None:intergenic | 35.0% | |
| ATCATCAAACCCAACAAAAC+CGG | - | 42700:16303-16322 | None:intergenic | 35.0% | |
| GAACTTAGGTTACTTGTTTG+AGG | + | 42700:16187-16206 | MS.gene82269:CDS | 35.0% | |
| GTTGTTTGAATATGGTGAAC+CGG | + | 42700:16412-16431 | MS.gene82269:CDS | 35.0% | |
| ! | AAGAAGAGATTTTTGCACCT+GGG | + | 42700:16087-16106 | MS.gene82269:CDS | 35.0% |
| ! | CAGTTGATACTAAGATTCGT+AGG | + | 42700:15868-15887 | MS.gene82269:CDS | 35.0% |
| !! | GGTTTTGATGACTTTGGTTA+TGG | + | 42700:16332-16351 | MS.gene82269:CDS | 35.0% |
| !!! | GGGTTTGATGATGATTTTAC+CGG | + | 42700:16311-16330 | MS.gene82269:CDS | 35.0% |
| !!! | TTTACCGGTTTTGATGACTT+TGG | + | 42700:16326-16345 | MS.gene82269:CDS | 35.0% |
| GATGACTTTGGTTATGGAAC+TGG | + | 42700:16338-16357 | MS.gene82269:CDS | 40.0% | |
| ! | AGGCTTCTGATGATGAACTT+GGG | + | 42700:16207-16226 | MS.gene82269:CDS | 40.0% |
| ! | GAAGAAGAGATTTTTGCACC+TGG | + | 42700:16086-16105 | MS.gene82269:CDS | 40.0% |
| !! | GTCGAGTCAGGTTTTTGATT+CGG | + | 42700:15965-15984 | MS.gene82269:CDS | 40.0% |
| !! | TTATGGAACTGGCTTGTTGT+CGG | + | 42700:16349-16368 | MS.gene82269:CDS | 40.0% |
| AACTCTCTTTCTCTTGTCGG+TGG | - | 42700:15811-15830 | None:intergenic | 45.0% | |
| ATCCCCAGTCACAAATTCTC+CGG | - | 42700:16390-16409 | None:intergenic | 45.0% | |
| GAGAATTTGTGACTGGGGAT+GGG | + | 42700:16390-16409 | MS.gene82269:CDS | 45.0% | |
| GGTGAGATGGAGATGAACTT+AGG | + | 42700:16173-16192 | MS.gene82269:CDS | 45.0% | |
| GTTTGAATATGGTGAACCGG+CGG | + | 42700:16415-16434 | MS.gene82269:CDS | 45.0% | |
| TGAACCGGGTTTGAATGAAC+CGG | + | 42700:16247-16266 | MS.gene82269:CDS | 45.0% | |
| ! | GAGGCTTCTGATGATGAACT+TGG | + | 42700:16206-16225 | MS.gene82269:CDS | 45.0% |
| !! | GGGGATGGGTTGTTTGAATA+TGG | + | 42700:16404-16423 | MS.gene82269:CDS | 45.0% |
| AAACCCGGTTCAGTGACAGT+TGG | - | 42700:16239-16258 | None:intergenic | 50.0% | |
| ACCGACCTGGTTCAAATGAC+CGG | + | 42700:16146-16165 | MS.gene82269:CDS | 50.0% | |
| ACCGGTCATTTGAACCAGGT+CGG | - | 42700:16150-16169 | None:intergenic | 50.0% | |
| CAAACCCAACAAAACCGGTC+AGG | - | 42700:16298-16317 | None:intergenic | 50.0% | |
| CATCTCCATCTCACCTGAAC+CGG | - | 42700:16168-16187 | None:intergenic | 50.0% | |
| CTGAACCGGTCATTTGAACC+AGG | - | 42700:16154-16173 | None:intergenic | 50.0% | |
| GAACCCGGTTCATTCAAACC+CGG | - | 42700:16254-16273 | None:intergenic | 50.0% | |
| GAACCGGGTTTGAATGAACC+GGG | + | 42700:16248-16267 | MS.gene82269:CDS | 50.0% | |
| GGAGAATTTGTGACTGGGGA+TGG | + | 42700:16389-16408 | MS.gene82269:CDS | 50.0% | |
| GGGTTTGAATGAACCGGGTT+CGG | + | 42700:16253-16272 | MS.gene82269:CDS | 50.0% | |
| GGTCCGGAGAATTTGTGACT+GGG | + | 42700:16384-16403 | MS.gene82269:CDS | 50.0% | |
| GTCCGGAGAATTTGTGACTG+GGG | + | 42700:16385-16404 | MS.gene82269:CDS | 50.0% | |
| TCAAGACCCATCACTGAGTC+AGG | - | 42700:16047-16066 | None:intergenic | 50.0% | |
| TCGACTCACTCGAGTCAACT+CGG | - | 42700:15950-15969 | None:intergenic | 50.0% | |
| TCTGAGCCTGACTCAGTGAT+GGG | + | 42700:16038-16057 | MS.gene82269:CDS | 50.0% | |
| TGAACCAGGTCTGACTGAAC+CGG | + | 42700:16112-16131 | MS.gene82269:CDS | 50.0% | |
| TTCTGAGCCTGACTCAGTGA+TGG | + | 42700:16037-16056 | MS.gene82269:CDS | 50.0% | |
| TTGTTGTCGGAGAGTGATGG+CGG | + | 42700:16362-16381 | MS.gene82269:CDS | 50.0% | |
| ! | AATGACCGGTTCAGGTGAGA+TGG | + | 42700:16160-16179 | MS.gene82269:CDS | 50.0% |
| ! | CTGGTTCAAATGACCGGTTC+AGG | + | 42700:16152-16171 | MS.gene82269:CDS | 50.0% |
| !!! | TGGACCTGACCGGTTTTGTT+GGG | + | 42700:16291-16310 | MS.gene82269:CDS | 50.0% |
| AGGTCTGACTGAACCGGAAC+CGG | + | 42700:16118-16137 | MS.gene82269:CDS | 55.0% | |
| CCACCAACTGTCACTGAACC+GGG | + | 42700:16233-16252 | MS.gene82269:CDS | 55.0% | |
| CGACTCACTCGAGTCAACTC+GGG | - | 42700:15949-15968 | None:intergenic | 55.0% | |
| GCCACCAACTGTCACTGAAC+CGG | + | 42700:16232-16251 | MS.gene82269:CDS | 55.0% | |
| GGGTCCGGAGAATTTGTGAC+TGG | + | 42700:16383-16402 | MS.gene82269:CDS | 55.0% | |
| TGAACCAGGTCGGTCTGAAC+CGG | - | 42700:16140-16159 | None:intergenic | 55.0% | |
| TGAATATGGTGAACCGGCGG+CGG | + | 42700:16418-16437 | MS.gene82269:CDS | 55.0% | |
| TGACTCGAGTGAGTCGAGTC+AGG | + | 42700:15953-15972 | MS.gene82269:CDS | 55.0% | |
| TGTTGTCGGAGAGTGATGGC+GGG | + | 42700:16363-16382 | MS.gene82269:CDS | 55.0% | |
| TTCGGTTGAACCGGAGAAGG+TGG | + | 42700:16271-16290 | MS.gene82269:CDS | 55.0% | |
| TTCTCCGGTTCAACCGAACC+CGG | - | 42700:16269-16288 | None:intergenic | 55.0% | |
| TTTGCACCTGGGACTGAACC+AGG | + | 42700:16098-16117 | MS.gene82269:CDS | 55.0% | |
| ! | TGAACCGGGTTCGGTTGAAC+CGG | + | 42700:16262-16281 | MS.gene82269:CDS | 55.0% |
| !! | GGCTTGTTGTCGGAGAGTGA+TGG | + | 42700:16359-16378 | MS.gene82269:CDS | 55.0% |
| !! | GTGGACCTGACCGGTTTTGT+TGG | + | 42700:16290-16309 | MS.gene82269:CDS | 55.0% |
| CCCGGTTCAGTGACAGTTGG+TGG | - | 42700:16236-16255 | None:intergenic | 60.0% | |
| GGTTCCGGTTCAGTCAGACC+TGG | - | 42700:16119-16138 | None:intergenic | 60.0% | |
| GTCAGACCTGGTTCAGTCCC+AGG | - | 42700:16107-16126 | None:intergenic | 60.0% | |
| ! | AGGTCGGTCTGAACCGGTTC+CGG | - | 42700:16134-16153 | None:intergenic | 60.0% |
| ! | GGCGGATGTTTTGTGGCGGT+CGG | + | 42700:16439-16458 | MS.gene82269:CDS | 60.0% |
| ! | GGGTTCGGTTGAACCGGAGA+AGG | + | 42700:16268-16287 | MS.gene82269:CDS | 60.0% |
| ATATGGTGAACCGGCGGCGG+CGG | + | 42700:16421-16440 | MS.gene82269:CDS | 65.0% | |
| CACAAAACATCCGCCGCCGC+CGG | - | 42700:16434-16453 | None:intergenic | 65.0% | |
| CCGGAGAAGGTGGACCTGAC+CGG | + | 42700:16281-16300 | MS.gene82269:CDS | 65.0% | |
| CCGGTCAGGTCCACCTTCTC+CGG | - | 42700:16284-16303 | None:intergenic | 65.0% | |
| ! | CGGCGGCGGATGTTTTGTGG+CGG | + | 42700:16435-16454 | MS.gene82269:CDS | 65.0% |
| ! | GGAACCGGTTCAGACCGACC+TGG | + | 42700:16133-16152 | MS.gene82269:CDS | 65.0% |
| !! | TCGGAGAGTGATGGCGGGTC+CGG | + | 42700:16368-16387 | MS.gene82269:CDS | 65.0% |
| ! | CGGCGGCGGCGGATGTTTTG+TGG | + | 42700:16432-16451 | MS.gene82269:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42700 | gene | 15798 | 16481 | 15798 | ID=MS.gene82269 |
| 42700 | mRNA | 15798 | 16481 | 15798 | ID=MS.gene82269.t1;Parent=MS.gene82269 |
| 42700 | exon | 15798 | 16481 | 15798 | ID=MS.gene82269.t1.exon1;Parent=MS.gene82269.t1 |
| 42700 | CDS | 15798 | 16481 | 15798 | ID=cds.MS.gene82269.t1;Parent=MS.gene82269.t1 |
| Gene Sequence |
| Protein sequence |
