Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32801.t1 | XP_003603170.1 | 100 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 8.30E-53 | 216.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32801.t1 | G7J8B4 | 100.0 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 5.9e-53 | 216.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene32801 | 0.813599 | 2.36E-51 | -1.69E-46 |
| MS.gene04920 | MS.gene32801 | 0.81387 | 2.06E-51 | -1.69E-46 |
| MS.gene049254 | MS.gene32801 | 0.850012 | 2.24E-60 | -1.69E-46 |
| MS.gene049283 | MS.gene32801 | 0.863929 | 1.75E-64 | -1.69E-46 |
| MS.gene049343 | MS.gene32801 | 0.803818 | 2.91E-49 | -1.69E-46 |
| MS.gene049419 | MS.gene32801 | -0.815702 | 8.07E-52 | -1.69E-46 |
| MS.gene049499 | MS.gene32801 | 0.837466 | 5.11E-57 | -1.69E-46 |
| MS.gene049567 | MS.gene32801 | 0.806883 | 6.63E-50 | -1.69E-46 |
| MS.gene049652 | MS.gene32801 | 0.850578 | 1.55E-60 | -1.69E-46 |
| MS.gene049680 | MS.gene32801 | 0.829862 | 4.06E-55 | -1.69E-46 |
| MS.gene049711 | MS.gene32801 | 0.800654 | 1.30E-48 | -1.69E-46 |
| MS.gene049983 | MS.gene32801 | 0.826801 | 2.22E-54 | -1.69E-46 |
| MS.gene050559 | MS.gene32801 | 0.838207 | 3.30E-57 | -1.69E-46 |
| MS.gene050561 | MS.gene32801 | 0.82987 | 4.04E-55 | -1.69E-46 |
| MS.gene050562 | MS.gene32801 | 0.828881 | 7.03E-55 | -1.69E-46 |
| MS.gene050571 | MS.gene32801 | 0.802321 | 5.93E-49 | -1.69E-46 |
| MS.gene051332 | MS.gene32801 | 0.80479 | 1.82E-49 | -1.69E-46 |
| MS.gene051492 | MS.gene32801 | 0.808479 | 3.03E-50 | -1.69E-46 |
| MS.gene051637 | MS.gene32801 | 0.818066 | 2.38E-52 | -1.69E-46 |
| MS.gene051699 | MS.gene32801 | 0.846924 | 1.60E-59 | -1.69E-46 |
| MS.gene051744 | MS.gene32801 | 0.821719 | 3.48E-53 | -1.69E-46 |
| MS.gene051831 | MS.gene32801 | 0.804941 | 1.70E-49 | -1.69E-46 |
| MS.gene051832 | MS.gene32801 | 0.847797 | 9.21E-60 | -1.69E-46 |
| MS.gene051848 | MS.gene32801 | -0.8108 | 9.63E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene32801 | 0.827715 | 1.34E-54 | -1.69E-46 |
| MS.gene052037 | MS.gene32801 | 0.808212 | 3.46E-50 | -1.69E-46 |
| MS.gene052118 | MS.gene32801 | 0.821708 | 3.50E-53 | -1.69E-46 |
| MS.gene052493 | MS.gene32801 | 0.817296 | 3.55E-52 | -1.69E-46 |
| MS.gene052533 | MS.gene32801 | 0.841979 | 3.42E-58 | -1.69E-46 |
| MS.gene052534 | MS.gene32801 | 0.819632 | 1.05E-52 | -1.69E-46 |
| MS.gene052685 | MS.gene32801 | 0.827077 | 1.91E-54 | -1.69E-46 |
| MS.gene052686 | MS.gene32801 | 0.815052 | 1.13E-51 | -1.69E-46 |
| MS.gene052717 | MS.gene32801 | 0.806432 | 8.25E-50 | -1.69E-46 |
| MS.gene052855 | MS.gene32801 | 0.804419 | 2.18E-49 | -1.69E-46 |
| MS.gene052889 | MS.gene32801 | 0.808245 | 3.41E-50 | -1.69E-46 |
| MS.gene053006 | MS.gene32801 | 0.801293 | 9.64E-49 | -1.69E-46 |
| MS.gene05308 | MS.gene32801 | 0.803687 | 3.09E-49 | -1.69E-46 |
| MS.gene053094 | MS.gene32801 | 0.869757 | 2.44E-66 | -1.69E-46 |
| MS.gene053298 | MS.gene32801 | 0.845198 | 4.72E-59 | -1.69E-46 |
| MS.gene053340 | MS.gene32801 | 0.828622 | 8.11E-55 | -1.69E-46 |
| MS.gene053963 | MS.gene32801 | 0.880624 | 4.71E-70 | -1.69E-46 |
| MS.gene053995 | MS.gene32801 | 0.804974 | 1.67E-49 | -1.69E-46 |
| MS.gene05410 | MS.gene32801 | 0.814207 | 1.73E-51 | -1.69E-46 |
| MS.gene054222 | MS.gene32801 | 0.873553 | 1.34E-67 | -1.69E-46 |
| MS.gene05423 | MS.gene32801 | 0.801171 | 1.02E-48 | -1.69E-46 |
| MS.gene05439 | MS.gene32801 | 0.80201 | 6.87E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene32801 | 0.811345 | 7.33E-51 | -1.69E-46 |
| MS.gene054576 | MS.gene32801 | 0.840704 | 7.41E-58 | -1.69E-46 |
| MS.gene054684 | MS.gene32801 | 0.815142 | 1.08E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene32801 | 0.865262 | 6.71E-65 | -1.69E-46 |
| MS.gene054893 | MS.gene32801 | 0.860343 | 2.21E-63 | -1.69E-46 |
| MS.gene054957 | MS.gene32801 | 0.831574 | 1.55E-55 | -1.69E-46 |
| MS.gene055141 | MS.gene32801 | 0.823649 | 1.24E-53 | -1.69E-46 |
| MS.gene055147 | MS.gene32801 | 0.83217 | 1.10E-55 | -1.69E-46 |
| MS.gene055148 | MS.gene32801 | 0.820359 | 7.15E-53 | -1.69E-46 |
| MS.gene055149 | MS.gene32801 | 0.832076 | 1.16E-55 | -1.69E-46 |
| MS.gene055150 | MS.gene32801 | 0.827681 | 1.37E-54 | -1.69E-46 |
| MS.gene055226 | MS.gene32801 | 0.815568 | 8.65E-52 | -1.69E-46 |
| MS.gene055229 | MS.gene32801 | 0.845742 | 3.36E-59 | -1.69E-46 |
| MS.gene055724 | MS.gene32801 | 0.832173 | 1.10E-55 | -1.69E-46 |
| MS.gene055801 | MS.gene32801 | 0.81718 | 3.77E-52 | -1.69E-46 |
| MS.gene05624 | MS.gene32801 | 0.837794 | 4.21E-57 | -1.69E-46 |
| MS.gene056315 | MS.gene32801 | 0.824108 | 9.65E-54 | -1.69E-46 |
| MS.gene056677 | MS.gene32801 | 0.848859 | 4.69E-60 | -1.69E-46 |
| MS.gene056699 | MS.gene32801 | 0.866822 | 2.15E-65 | -1.69E-46 |
| MS.gene05686 | MS.gene32801 | 0.835512 | 1.61E-56 | -1.69E-46 |
| MS.gene056973 | MS.gene32801 | 0.807238 | 5.57E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene32801 | 0.828662 | 7.94E-55 | -1.69E-46 |
| MS.gene057034 | MS.gene32801 | 0.80604 | 9.98E-50 | -1.69E-46 |
| MS.gene057071 | MS.gene32801 | 0.812406 | 4.31E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene32801 | 0.866773 | 2.23E-65 | -1.69E-46 |
| MS.gene057155 | MS.gene32801 | 0.837005 | 6.71E-57 | -1.69E-46 |
| MS.gene057208 | MS.gene32801 | 0.847768 | 9.38E-60 | -1.69E-46 |
| MS.gene057622 | MS.gene32801 | 0.815171 | 1.06E-51 | -1.69E-46 |
| MS.gene057999 | MS.gene32801 | 0.804889 | 1.74E-49 | -1.69E-46 |
| MS.gene058095 | MS.gene32801 | 0.800784 | 1.22E-48 | -1.69E-46 |
| MS.gene058159 | MS.gene32801 | 0.83981 | 1.27E-57 | -1.69E-46 |
| MS.gene058279 | MS.gene32801 | 0.868932 | 4.52E-66 | -1.69E-46 |
| MS.gene058592 | MS.gene32801 | 0.878158 | 3.52E-69 | -1.69E-46 |
| MS.gene058635 | MS.gene32801 | 0.804936 | 1.70E-49 | -1.69E-46 |
| MS.gene058781 | MS.gene32801 | 0.80547 | 1.31E-49 | -1.69E-46 |
| MS.gene05878 | MS.gene32801 | 0.803679 | 3.11E-49 | -1.69E-46 |
| MS.gene058897 | MS.gene32801 | 0.806434 | 8.24E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene32801 | 0.801238 | 9.90E-49 | -1.69E-46 |
| MS.gene059051 | MS.gene32801 | 0.846527 | 2.05E-59 | -1.69E-46 |
| MS.gene059170 | MS.gene32801 | 0.817458 | 3.26E-52 | -1.69E-46 |
| MS.gene05952 | MS.gene32801 | 0.88566 | 6.71E-72 | -1.69E-46 |
| MS.gene05956 | MS.gene32801 | 0.806083 | 9.77E-50 | -1.69E-46 |
| MS.gene059774 | MS.gene32801 | 0.842407 | 2.64E-58 | -1.69E-46 |
| MS.gene05985 | MS.gene32801 | -0.889598 | 2.10E-73 | -1.69E-46 |
| MS.gene059901 | MS.gene32801 | -0.822516 | 2.27E-53 | -1.69E-46 |
| MS.gene059935 | MS.gene32801 | 0.826372 | 2.81E-54 | -1.69E-46 |
| MS.gene059965 | MS.gene32801 | 0.818776 | 1.64E-52 | -1.69E-46 |
| MS.gene060012 | MS.gene32801 | 0.858275 | 9.21E-63 | -1.69E-46 |
| MS.gene060244 | MS.gene32801 | 0.805919 | 1.06E-49 | -1.69E-46 |
| MS.gene060257 | MS.gene32801 | 0.806962 | 6.37E-50 | -1.69E-46 |
| MS.gene060338 | MS.gene32801 | 0.808193 | 3.49E-50 | -1.69E-46 |
| MS.gene060365 | MS.gene32801 | 0.840374 | 9.04E-58 | -1.69E-46 |
| MS.gene060373 | MS.gene32801 | 0.805605 | 1.23E-49 | -1.69E-46 |
| MS.gene060412 | MS.gene32801 | 0.855592 | 5.69E-62 | -1.69E-46 |
| MS.gene060413 | MS.gene32801 | 0.800125 | 1.67E-48 | -1.69E-46 |
| MS.gene060520 | MS.gene32801 | 0.802523 | 5.39E-49 | -1.69E-46 |
| MS.gene060527 | MS.gene32801 | 0.833059 | 6.63E-56 | -1.69E-46 |
| MS.gene060697 | MS.gene32801 | 0.837849 | 4.08E-57 | -1.69E-46 |
| MS.gene060716 | MS.gene32801 | 0.809178 | 2.15E-50 | -1.69E-46 |
| MS.gene060777 | MS.gene32801 | 0.828285 | 9.79E-55 | -1.69E-46 |
| MS.gene060840 | MS.gene32801 | 0.842145 | 3.09E-58 | -1.69E-46 |
| MS.gene060879 | MS.gene32801 | 0.804861 | 1.76E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene32801 | 0.806743 | 7.09E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene32801 | 0.812785 | 3.56E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene32801 | 0.831709 | 1.43E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene32801 | 0.846424 | 2.19E-59 | -1.69E-46 |
| MS.gene061047 | MS.gene32801 | 0.830743 | 2.47E-55 | -1.69E-46 |
| MS.gene061100 | MS.gene32801 | 0.809445 | 1.89E-50 | -1.69E-46 |
| MS.gene061188 | MS.gene32801 | 0.858748 | 6.66E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32801.t1 | MTR_3g104640 | 100.000 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 1.63e-74 | 216 |
| MS.gene32801.t1 | MTR_4g125990 | 55.906 | 127 | 35 | 5 | 1 | 110 | 1 | 123 | 8.48e-35 | 116 |
| MS.gene32801.t1 | MTR_1g015810 | 67.241 | 116 | 30 | 5 | 1 | 108 | 1 | 116 | 6.41e-32 | 108 |
| MS.gene32801.t1 | MTR_2g099630 | 49.541 | 109 | 44 | 4 | 6 | 109 | 4 | 106 | 4.69e-26 | 93.6 |
| MS.gene32801.t1 | MTR_8g018320 | 50.495 | 101 | 49 | 1 | 6 | 106 | 4 | 103 | 6.89e-22 | 83.2 |
| MS.gene32801.t1 | MTR_8g018360 | 50.000 | 102 | 50 | 1 | 6 | 107 | 4 | 104 | 7.16e-21 | 82.8 |
| MS.gene32801.t1 | MTR_7g109290 | 46.667 | 60 | 32 | 0 | 51 | 110 | 32 | 91 | 5.29e-14 | 62.4 |
| MS.gene32801.t1 | MTR_1g098310 | 57.407 | 54 | 20 | 1 | 56 | 109 | 35 | 85 | 2.54e-13 | 60.8 |
| MS.gene32801.t1 | MTR_1g087730 | 42.373 | 59 | 34 | 0 | 52 | 110 | 48 | 106 | 9.62e-13 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32801.t1 | AT5G11970 | 59.091 | 110 | 39 | 3 | 1 | 110 | 1 | 104 | 1.79e-35 | 117 |
| MS.gene32801.t1 | AT2G19460 | 45.455 | 121 | 46 | 5 | 1 | 107 | 1 | 115 | 4.13e-21 | 81.6 |
| MS.gene32801.t1 | AT2G19460 | 45.455 | 121 | 46 | 5 | 1 | 107 | 53 | 167 | 1.95e-20 | 81.3 |
| MS.gene32801.t1 | AT2G47480 | 51.613 | 62 | 27 | 1 | 50 | 108 | 47 | 108 | 5.75e-15 | 65.5 |
| MS.gene32801.t1 | AT3G13910 | 58.621 | 58 | 23 | 1 | 45 | 101 | 40 | 97 | 2.48e-14 | 63.9 |
| MS.gene32801.t1 | AT3G13910 | 58.621 | 58 | 23 | 1 | 45 | 101 | 40 | 97 | 5.38e-14 | 63.2 |
| MS.gene32801.t1 | AT1G72720 | 62.222 | 45 | 17 | 0 | 65 | 109 | 81 | 125 | 6.28e-14 | 63.5 |
| MS.gene32801.t1 | AT3G62640 | 51.111 | 45 | 22 | 0 | 65 | 109 | 66 | 110 | 6.18e-13 | 60.5 |
| MS.gene32801.t1 | AT3G05725 | 35.115 | 131 | 55 | 7 | 1 | 109 | 1 | 123 | 1.61e-12 | 59.7 |
| MS.gene32801.t1 | AT4G09890 | 53.846 | 39 | 18 | 0 | 66 | 104 | 39 | 77 | 5.58e-11 | 54.7 |
Find 21 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTACTCACAGGTGGTTTA+TGG | 0.228498 | 3.1:-82510144 | MS.gene32801:CDS |
| CTCTTCTTCCTCTGAAGTTC+AGG | 0.289847 | 3.1:+82510246 | None:intergenic |
| AGCTTTCTATCTGCATCATT+TGG | 0.373432 | 3.1:+82510409 | None:intergenic |
| TATAAAATGTATTCTGTTGA+AGG | 0.385236 | 3.1:-82510213 | MS.gene32801:CDS |
| CTAAGATCATAAGGCTTAGA+TGG | 0.404348 | 3.1:+82510378 | None:intergenic |
| ATGCAGATAGAAAGCTACTA+TGG | 0.407129 | 3.1:-82510402 | MS.gene32801:CDS |
| TATCTGCATCATTTGGCCAT+TGG | 0.440208 | 3.1:+82510416 | None:intergenic |
| CTATAGCTTCTAAGATCATA+AGG | 0.444134 | 3.1:+82510369 | None:intergenic |
| CTGAACTTCAGAGGAAGAAG+AGG | 0.478478 | 3.1:-82510245 | MS.gene32801:CDS |
| TTCTTCAACTTCAAATCCTT+AGG | 0.481282 | 3.1:+82510324 | None:intergenic |
| AAGGATTTGAAGTTGAAGAA+AGG | 0.511800 | 3.1:-82510321 | MS.gene32801:CDS |
| CTTAGGAGTTTGTTGTACAT+AGG | 0.522202 | 3.1:+82510341 | None:intergenic |
| CTATGTACAACAAACTCCTA+AGG | 0.555597 | 3.1:-82510340 | MS.gene32801:CDS |
| TCTGTTGAAGGCAAAGTCAA+AGG | 0.571369 | 3.1:-82510201 | MS.gene32801:CDS |
| CTTTCAGAAAGAGTTTCAGA+TGG | 0.576426 | 3.1:-82510176 | MS.gene32801:CDS |
| ACTCACAGGTGGTTTATGGT+TGG | 0.580184 | 3.1:-82510140 | MS.gene32801:CDS |
| TGTCTGATCCTGAACTTCAG+AGG | 0.591643 | 3.1:-82510254 | MS.gene32801:CDS |
| AGATCAAAATCTTATGCCAA+TGG | 0.604385 | 3.1:-82510432 | MS.gene32801:CDS |
| GCTTAAGAACAAGTACTCAC+AGG | 0.640169 | 3.1:-82510154 | MS.gene32801:CDS |
| TGAACTTCAGAGGAAGAAGA+GGG | 0.657165 | 3.1:-82510244 | MS.gene32801:CDS |
| TAAGAACAAGTACTCACAGG+TGG | 0.685797 | 3.1:-82510151 | MS.gene32801:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAAATGTATTCTGTTGA+AGG | - | chr3.1:82510371-82510390 | MS.gene32801:CDS | 20.0% |
| AGATCAAAATCTTATGCCAA+TGG | - | chr3.1:82510152-82510171 | MS.gene32801:CDS | 30.0% | |
| TTCTTCAACTTCAAATCCTT+AGG | + | chr3.1:82510263-82510282 | None:intergenic | 30.0% | |
| ! | AAAGGTAAAAGCTTTTCATC+TGG | - | chr3.1:82510281-82510300 | MS.gene32801:CDS | 30.0% |
| ! | AAGGATTTGAAGTTGAAGAA+AGG | - | chr3.1:82510263-82510282 | MS.gene32801:CDS | 30.0% |
| !! | CTATAGCTTCTAAGATCATA+AGG | + | chr3.1:82510218-82510237 | None:intergenic | 30.0% |
| AGCTTTCTATCTGCATCATT+TGG | + | chr3.1:82510178-82510197 | None:intergenic | 35.0% | |
| ATGCAGATAGAAAGCTACTA+TGG | - | chr3.1:82510182-82510201 | MS.gene32801:CDS | 35.0% | |
| CTAAGATCATAAGGCTTAGA+TGG | + | chr3.1:82510209-82510228 | None:intergenic | 35.0% | |
| CTATGTACAACAAACTCCTA+AGG | - | chr3.1:82510244-82510263 | MS.gene32801:CDS | 35.0% | |
| CTTTCAGAAAGAGTTTCAGA+TGG | - | chr3.1:82510408-82510427 | MS.gene32801:CDS | 35.0% | |
| !! | CTTAGGAGTTTGTTGTACAT+AGG | + | chr3.1:82510246-82510265 | None:intergenic | 35.0% |
| AAGTACTCACAGGTGGTTTA+TGG | - | chr3.1:82510440-82510459 | MS.gene32801:CDS | 40.0% | |
| GCTTAAGAACAAGTACTCAC+AGG | - | chr3.1:82510430-82510449 | MS.gene32801:CDS | 40.0% | |
| TAAGAACAAGTACTCACAGG+TGG | - | chr3.1:82510433-82510452 | MS.gene32801:CDS | 40.0% | |
| TATCTGCATCATTTGGCCAT+TGG | + | chr3.1:82510171-82510190 | None:intergenic | 40.0% | |
| TCTGTTGAAGGCAAAGTCAA+AGG | - | chr3.1:82510383-82510402 | MS.gene32801:CDS | 40.0% | |
| TGAACTTCAGAGGAAGAAGA+GGG | - | chr3.1:82510340-82510359 | MS.gene32801:CDS | 40.0% | |
| ACTCACAGGTGGTTTATGGT+TGG | - | chr3.1:82510444-82510463 | MS.gene32801:CDS | 45.0% | |
| CTGAACTTCAGAGGAAGAAG+AGG | - | chr3.1:82510339-82510358 | MS.gene32801:CDS | 45.0% | |
| TGTCTGATCCTGAACTTCAG+AGG | - | chr3.1:82510330-82510349 | MS.gene32801:CDS | 45.0% | |
| !! | CTCTTCTTCCTCTGAAGTTC+AGG | + | chr3.1:82510341-82510360 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 82510137 | 82510469 | 82510137 | ID=MS.gene32801 |
| chr3.1 | mRNA | 82510137 | 82510469 | 82510137 | ID=MS.gene32801.t1;Parent=MS.gene32801 |
| chr3.1 | exon | 82510137 | 82510469 | 82510137 | ID=MS.gene32801.t1.exon1;Parent=MS.gene32801.t1 |
| chr3.1 | CDS | 82510137 | 82510469 | 82510137 | ID=cds.MS.gene32801.t1;Parent=MS.gene32801.t1 |
| Gene Sequence |
| Protein sequence |
