Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72620.t1 | XP_024641403.1 | 95.7 | 69 | 3 | 0 | 4 | 72 | 1 | 69 | 1.60E-28 | 134.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72620.t1 | G7KQA5 | 95.5 | 66 | 3 | 0 | 4 | 69 | 1 | 66 | 6.3e-27 | 129.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04919 | MS.gene72620 | 0.804614 | 1.98E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene72620 | 0.827862 | 1.24E-54 | -1.69E-46 |
| MS.gene049283 | MS.gene72620 | 0.860177 | 2.48E-63 | -1.69E-46 |
| MS.gene049419 | MS.gene72620 | -0.839509 | 1.52E-57 | -1.69E-46 |
| MS.gene049499 | MS.gene72620 | 0.839508 | 1.52E-57 | -1.69E-46 |
| MS.gene049680 | MS.gene72620 | 0.806751 | 7.07E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene72620 | 0.814588 | 1.43E-51 | -1.69E-46 |
| MS.gene050271 | MS.gene72620 | 0.82235 | 2.48E-53 | -1.69E-46 |
| MS.gene050646 | MS.gene72620 | 0.800923 | 1.15E-48 | -1.69E-46 |
| MS.gene050945 | MS.gene72620 | 0.802362 | 5.82E-49 | -1.69E-46 |
| MS.gene051043 | MS.gene72620 | 0.806039 | 9.98E-50 | -1.69E-46 |
| MS.gene051275 | MS.gene72620 | 0.808932 | 2.43E-50 | -1.69E-46 |
| MS.gene051597 | MS.gene72620 | 0.818833 | 1.59E-52 | -1.69E-46 |
| MS.gene051699 | MS.gene72620 | 0.819179 | 1.33E-52 | -1.69E-46 |
| MS.gene051832 | MS.gene72620 | 0.818885 | 1.55E-52 | -1.69E-46 |
| MS.gene051902 | MS.gene72620 | 0.820201 | 7.77E-53 | -1.69E-46 |
| MS.gene052954 | MS.gene72620 | 0.801008 | 1.10E-48 | -1.69E-46 |
| MS.gene053045 | MS.gene72620 | 0.800761 | 1.24E-48 | -1.69E-46 |
| MS.gene053094 | MS.gene72620 | 0.803888 | 2.81E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene72620 | 0.808192 | 3.49E-50 | -1.69E-46 |
| MS.gene053298 | MS.gene72620 | 0.8233 | 1.49E-53 | -1.69E-46 |
| MS.gene053340 | MS.gene72620 | 0.810553 | 1.09E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene72620 | 0.808747 | 2.66E-50 | -1.69E-46 |
| MS.gene053879 | MS.gene72620 | 0.813691 | 2.25E-51 | -1.69E-46 |
| MS.gene053931 | MS.gene72620 | 0.808615 | 2.84E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene72620 | 0.87364 | 1.26E-67 | -1.69E-46 |
| MS.gene054222 | MS.gene72620 | 0.825692 | 4.08E-54 | -1.69E-46 |
| MS.gene054892 | MS.gene72620 | 0.828861 | 7.11E-55 | -1.69E-46 |
| MS.gene054893 | MS.gene72620 | 0.831458 | 1.65E-55 | -1.69E-46 |
| MS.gene054957 | MS.gene72620 | 0.811495 | 6.81E-51 | -1.69E-46 |
| MS.gene055149 | MS.gene72620 | 0.810058 | 1.39E-50 | -1.69E-46 |
| MS.gene055150 | MS.gene72620 | 0.815866 | 7.42E-52 | -1.69E-46 |
| MS.gene055841 | MS.gene72620 | 0.803869 | 2.84E-49 | -1.69E-46 |
| MS.gene056068 | MS.gene72620 | 0.802993 | 4.31E-49 | -1.69E-46 |
| MS.gene056100 | MS.gene72620 | 0.803142 | 4.02E-49 | -1.69E-46 |
| MS.gene056973 | MS.gene72620 | 0.81971 | 1.01E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene72620 | 0.802307 | 5.97E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene72620 | 0.848858 | 4.69E-60 | -1.69E-46 |
| MS.gene057622 | MS.gene72620 | 0.822073 | 2.88E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene72620 | 0.826359 | 2.83E-54 | -1.69E-46 |
| MS.gene058159 | MS.gene72620 | 0.826234 | 3.03E-54 | -1.69E-46 |
| MS.gene058279 | MS.gene72620 | 0.854803 | 9.65E-62 | -1.69E-46 |
| MS.gene058592 | MS.gene72620 | 0.823273 | 1.51E-53 | -1.69E-46 |
| MS.gene059051 | MS.gene72620 | 0.802385 | 5.75E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene72620 | 0.853669 | 2.05E-61 | -1.69E-46 |
| MS.gene05985 | MS.gene72620 | -0.84712 | 1.41E-59 | -1.69E-46 |
| MS.gene060012 | MS.gene72620 | 0.800118 | 1.67E-48 | -1.69E-46 |
| MS.gene06018 | MS.gene72620 | 0.821164 | 4.67E-53 | -1.69E-46 |
| MS.gene060244 | MS.gene72620 | 0.827181 | 1.80E-54 | -1.69E-46 |
| MS.gene060365 | MS.gene72620 | 0.810854 | 9.37E-51 | -1.69E-46 |
| MS.gene060527 | MS.gene72620 | 0.83417 | 3.50E-56 | -1.69E-46 |
| MS.gene060935 | MS.gene72620 | 0.807953 | 3.93E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene72620 | 0.824401 | 8.23E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72620.t1 | MTR_6g071040 | 95.455 | 66 | 3 | 0 | 4 | 69 | 1 | 66 | 4.71e-40 | 127 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72620.t1 | AT1G67920 | 54.545 | 66 | 28 | 2 | 7 | 71 | 3 | 67 | 1.18e-11 | 54.7 |
Find 23 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTCCCCGTTGGGAATTTC+AGG | 0.186797 | 6.4:+48467742 | MS.gene72620:CDS |
| CTGAGATAAGACCTGTTCTA+AGG | 0.312109 | 6.4:+48467698 | MS.gene72620:CDS |
| TTCACAACTTGACTATGAAT+TGG | 0.323676 | 6.4:-48467628 | None:intergenic |
| CGAATTTCGCGGTCCCCGTT+GGG | 0.367639 | 6.4:+48467733 | MS.gene72620:CDS |
| ACGAATTTCGCGGTCCCCGT+TGG | 0.391377 | 6.4:+48467732 | MS.gene72620:CDS |
| CTTAGAACAGGTCTTATCTC+AGG | 0.409951 | 6.4:-48467697 | None:intergenic |
| GGATGAAGGTGTTGGACAAA+AGG | 0.436976 | 6.4:+48467600 | MS.gene72620:CDS |
| CGCGAAATCTCCCTTAGAAC+AGG | 0.449387 | 6.4:-48467709 | None:intergenic |
| CTTGGCCTGAAATTCCCAAC+GGG | 0.491052 | 6.4:-48467747 | None:intergenic |
| GATGAAGGTGTTGGACAAAA+GGG | 0.511350 | 6.4:+48467601 | MS.gene72620:CDS |
| TCAGGCCAAGCTATTTCAGT+TGG | 0.530276 | 6.4:+48467760 | MS.gene72620:CDS |
| AATCACCAACTGAAATAGCT+TGG | 0.541732 | 6.4:-48467765 | None:intergenic |
| AAGAGTAACAAGAAGGATGA+AGG | 0.544397 | 6.4:+48467586 | MS.gene72620:CDS |
| AATCAGAGAAAGTATTCGAT+TGG | 0.545615 | 6.4:+48467662 | MS.gene72620:CDS |
| GGTCTTATCTCAGGTTTACC+AGG | 0.545864 | 6.4:-48467688 | None:intergenic |
| TTTCAGTTGGTGATTCATAG+AGG | 0.548698 | 6.4:+48467773 | MS.gene72620:CDS |
| AAAGTATTCGATTGGTCACC+TGG | 0.550724 | 6.4:+48467670 | MS.gene72620:CDS |
| AGATTTCGCGACGAATTTCG+CGG | 0.565935 | 6.4:+48467722 | MS.gene72620:CDS |
| TGAGATAAGACCTGTTCTAA+GGG | 0.567147 | 6.4:+48467699 | MS.gene72620:CDS |
| AACAAGAAGGATGAAGGTGT+TGG | 0.575319 | 6.4:+48467592 | MS.gene72620:CDS |
| GAACATGAAGAGTAACAAGA+AGG | 0.578943 | 6.4:+48467579 | MS.gene72620:CDS |
| GCTTGGCCTGAAATTCCCAA+CGG | 0.666118 | 6.4:-48467748 | None:intergenic |
| TTGGCCTGAAATTCCCAACG+GGG | 0.805867 | 6.4:-48467746 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCACAACTTGACTATGAAT+TGG | - | chr6.4:48467631-48467650 | None:intergenic | 30.0% | |
| !! | AATCAGAGAAAGTATTCGAT+TGG | + | chr6.4:48467662-48467681 | MS.gene72620:CDS | 30.0% |
| AAGAGTAACAAGAAGGATGA+AGG | + | chr6.4:48467586-48467605 | MS.gene72620:CDS | 35.0% | |
| AATCACCAACTGAAATAGCT+TGG | - | chr6.4:48467768-48467787 | None:intergenic | 35.0% | |
| GAACATGAAGAGTAACAAGA+AGG | + | chr6.4:48467579-48467598 | MS.gene72620:CDS | 35.0% | |
| TGAGATAAGACCTGTTCTAA+GGG | + | chr6.4:48467699-48467718 | MS.gene72620:CDS | 35.0% | |
| CTGAGATAAGACCTGTTCTA+AGG | + | chr6.4:48467698-48467717 | MS.gene72620:CDS | 40.0% | |
| ! | AAAGTATTCGATTGGTCACC+TGG | + | chr6.4:48467670-48467689 | MS.gene72620:CDS | 40.0% |
| ! | AACAAGAAGGATGAAGGTGT+TGG | + | chr6.4:48467592-48467611 | MS.gene72620:CDS | 40.0% |
| ! | CTTAGAACAGGTCTTATCTC+AGG | - | chr6.4:48467700-48467719 | None:intergenic | 40.0% |
| !! | GATGAAGGTGTTGGACAAAA+GGG | + | chr6.4:48467601-48467620 | MS.gene72620:CDS | 40.0% |
| AGATTTCGCGACGAATTTCG+CGG | + | chr6.4:48467722-48467741 | MS.gene72620:CDS | 45.0% | |
| ! | GGTCTTATCTCAGGTTTACC+AGG | - | chr6.4:48467691-48467710 | None:intergenic | 45.0% |
| ! | TCAGGCCAAGCTATTTCAGT+TGG | + | chr6.4:48467760-48467779 | MS.gene72620:CDS | 45.0% |
| !! | GGATGAAGGTGTTGGACAAA+AGG | + | chr6.4:48467600-48467619 | MS.gene72620:CDS | 45.0% |
| CGCGAAATCTCCCTTAGAAC+AGG | - | chr6.4:48467712-48467731 | None:intergenic | 50.0% | |
| CTTGGCCTGAAATTCCCAAC+GGG | - | chr6.4:48467750-48467769 | None:intergenic | 50.0% | |
| GCTTGGCCTGAAATTCCCAA+CGG | - | chr6.4:48467751-48467770 | None:intergenic | 50.0% | |
| TTGGCCTGAAATTCCCAACG+GGG | - | chr6.4:48467749-48467768 | None:intergenic | 50.0% | |
| ACGAATTTCGCGGTCCCCGT+TGG | + | chr6.4:48467732-48467751 | MS.gene72620:CDS | 60.0% | |
| CGAATTTCGCGGTCCCCGTT+GGG | + | chr6.4:48467733-48467752 | MS.gene72620:CDS | 60.0% | |
| CGGTCCCCGTTGGGAATTTC+AGG | + | chr6.4:48467742-48467761 | MS.gene72620:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 48467574 | 48467792 | 48467574 | ID=MS.gene72620 |
| chr6.4 | mRNA | 48467574 | 48467792 | 48467574 | ID=MS.gene72620.t1;Parent=MS.gene72620 |
| chr6.4 | exon | 48467574 | 48467792 | 48467574 | ID=MS.gene72620.t1.exon1;Parent=MS.gene72620.t1 |
| chr6.4 | CDS | 48467574 | 48467792 | 48467574 | ID=cds.MS.gene72620.t1;Parent=MS.gene72620.t1 |
| Gene Sequence |
| Protein sequence |
