AlfalfaGEDB | MS.gene34444

M. sativa cultivar XinJiangDaYe / MS.gene34444

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34444.t1	XP_003592328.1	99	104	1	0	1	104	1	104	3.90E-52	213.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34444.t1	G7IC10	99.0	104	1	0	1	104	1	104	2.8e-52	213.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene34444	0.82437	8.37E-54	-1.69E-46
MS.gene049419	MS.gene34444	-0.837067	6.47E-57	-1.69E-46
MS.gene049499	MS.gene34444	0.806647	7.43E-50	-1.69E-46
MS.gene049567	MS.gene34444	0.806847	6.74E-50	-1.69E-46
MS.gene049652	MS.gene34444	0.810279	1.25E-50	-1.69E-46
MS.gene049669	MS.gene34444	0.836398	9.58E-57	-1.69E-46
MS.gene050559	MS.gene34444	0.821857	3.23E-53	-1.69E-46
MS.gene050561	MS.gene34444	0.819919	9.02E-53	-1.69E-46
MS.gene050562	MS.gene34444	0.818638	1.77E-52	-1.69E-46
MS.gene050780	MS.gene34444	0.814915	1.21E-51	-1.69E-46
MS.gene051279	MS.gene34444	0.800613	1.33E-48	-1.69E-46
MS.gene051332	MS.gene34444	0.826059	3.34E-54	-1.69E-46
MS.gene051490	MS.gene34444	0.811259	7.65E-51	-1.69E-46
MS.gene051492	MS.gene34444	0.845187	4.75E-59	-1.69E-46
MS.gene051744	MS.gene34444	0.810049	1.40E-50	-1.69E-46
MS.gene051831	MS.gene34444	0.803474	3.43E-49	-1.69E-46
MS.gene051832	MS.gene34444	0.825935	3.57E-54	-1.69E-46
MS.gene052176	MS.gene34444	0.804924	1.71E-49	-1.69E-46
MS.gene052493	MS.gene34444	0.809231	2.10E-50	-1.69E-46
MS.gene052500	MS.gene34444	0.804876	1.75E-49	-1.69E-46
MS.gene052502	MS.gene34444	0.800693	1.28E-48	-1.69E-46
MS.gene052534	MS.gene34444	0.805827	1.11E-49	-1.69E-46
MS.gene052582	MS.gene34444	0.801875	7.33E-49	-1.69E-46
MS.gene052586	MS.gene34444	0.850851	1.30E-60	-1.69E-46
MS.gene052685	MS.gene34444	0.820408	6.97E-53	-1.69E-46
MS.gene052826	MS.gene34444	0.802919	4.46E-49	-1.69E-46
MS.gene052855	MS.gene34444	0.801507	8.72E-49	-1.69E-46
MS.gene052954	MS.gene34444	0.811544	6.64E-51	-1.69E-46
MS.gene053006	MS.gene34444	0.829871	4.04E-55	-1.69E-46
MS.gene053094	MS.gene34444	0.888065	8.21E-73	-1.69E-46
MS.gene053189	MS.gene34444	0.810972	8.84E-51	-1.69E-46
MS.gene053298	MS.gene34444	0.822025	2.95E-53	-1.69E-46
MS.gene053340	MS.gene34444	0.852809	3.62E-61	-1.69E-46
MS.gene053647	MS.gene34444	0.800011	1.76E-48	-1.69E-46
MS.gene053963	MS.gene34444	0.852427	4.65E-61	-1.69E-46
MS.gene05410	MS.gene34444	0.826313	2.91E-54	-1.69E-46
MS.gene05414	MS.gene34444	0.809467	1.87E-50	-1.69E-46
MS.gene05415	MS.gene34444	0.831144	1.97E-55	-1.69E-46
MS.gene054222	MS.gene34444	0.841266	5.28E-58	-1.69E-46
MS.gene054479	MS.gene34444	0.801896	7.25E-49	-1.69E-46
MS.gene054576	MS.gene34444	0.848245	6.93E-60	-1.69E-46
MS.gene05469	MS.gene34444	0.804283	2.33E-49	-1.69E-46
MS.gene054892	MS.gene34444	0.837502	5.01E-57	-1.69E-46
MS.gene054893	MS.gene34444	0.837262	5.77E-57	-1.69E-46
MS.gene055141	MS.gene34444	0.804864	1.76E-49	-1.69E-46
MS.gene055147	MS.gene34444	0.827245	1.74E-54	-1.69E-46
MS.gene055148	MS.gene34444	0.809283	2.04E-50	-1.69E-46
MS.gene055149	MS.gene34444	0.814221	1.72E-51	-1.69E-46
MS.gene055225	MS.gene34444	0.814763	1.31E-51	-1.69E-46
MS.gene055226	MS.gene34444	0.82194	3.09E-53	-1.69E-46
MS.gene056506	MS.gene34444	0.800196	1.61E-48	-1.69E-46
MS.gene05686	MS.gene34444	0.809036	2.31E-50	-1.69E-46
MS.gene057026	MS.gene34444	0.811058	8.46E-51	-1.69E-46
MS.gene057208	MS.gene34444	0.835527	1.59E-56	-1.69E-46
MS.gene058037	MS.gene34444	0.805361	1.39E-49	-1.69E-46
MS.gene058477	MS.gene34444	0.807071	6.04E-50	-1.69E-46
MS.gene059051	MS.gene34444	0.82685	2.16E-54	-1.69E-46
MS.gene059272	MS.gene34444	0.805785	1.13E-49	-1.69E-46
MS.gene059439	MS.gene34444	0.812913	3.34E-51	-1.69E-46
MS.gene05952	MS.gene34444	0.836702	8.01E-57	-1.69E-46
MS.gene059774	MS.gene34444	0.838211	3.29E-57	-1.69E-46
MS.gene05985	MS.gene34444	-0.810331	1.22E-50	-1.69E-46
MS.gene060012	MS.gene34444	0.828668	7.91E-55	-1.69E-46
MS.gene060365	MS.gene34444	0.81579	7.72E-52	-1.69E-46
MS.gene060373	MS.gene34444	0.805554	1.26E-49	-1.69E-46
MS.gene060412	MS.gene34444	0.834246	3.35E-56	-1.69E-46
MS.gene060697	MS.gene34444	0.835888	1.29E-56	-1.69E-46
MS.gene060716	MS.gene34444	0.809307	2.02E-50	-1.69E-46
MS.gene060840	MS.gene34444	0.818407	1.99E-52	-1.69E-46
MS.gene060879	MS.gene34444	0.863216	2.92E-64	-1.69E-46
MS.gene061047	MS.gene34444	0.818461	1.94E-52	-1.69E-46
MS.gene061188	MS.gene34444	0.810675	1.02E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34444.t1	MTR_1g101680	99.038	104	1	0	1	104	1	104	6.79e-74	214
MS.gene34444.t1	MTR_1g101750	98.361	61	1	0	44	104	6	66	8.73e-41	129
MS.gene34444.t1	MTR_8g043660	61.458	96	31	2	6	96	18	112	1.39e-37	122
MS.gene34444.t1	MTR_8g032890	60.417	96	32	2	6	96	18	112	1.44e-35	117
MS.gene34444.t1	MTR_7g104250	63.333	90	31	1	10	97	6	95	4.10e-32	108
MS.gene34444.t1	MTR_1g060430	51.923	104	36	3	2	92	7	109	5.59e-31	106
MS.gene34444.t1	MTR_8g032940	63.043	92	29	1	6	92	18	109	1.02e-30	105
MS.gene34444.t1	MTR_5g038200	44.928	69	35	1	16	84	9	74	2.89e-15	65.1
MS.gene34444.t1	MTR_7g055590	74.286	35	9	0	59	93	461	495	1.31e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34444.t1	AT4G25433	52.525	99	36	3	4	92	7	104	8.36e-30	103
MS.gene34444.t1	AT5G62150	58.947	95	37	2	7	101	10	102	9.28e-29	100
MS.gene34444.t1	AT3G52790	51.485	101	37	3	6	96	10	108	3.23e-28	99.0

Find 25 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTATTGGGCATGATATTAT+TGG	0.221140	1.2:+75920710	MS.gene34444:CDS
GTATTGTGCAATAGCGTTAT+TGG	0.257022	1.2:+75920695	MS.gene34444:CDS
CTTCAACAATTTCCTAGTAT+TGG	0.339584	1.2:-75920949	None:intergenic
TTATTGGGCATGATATTATT+GGG	0.350044	1.2:+75920711	MS.gene34444:CDS
GGATTCCTCTCTACAATAAA+CGG	0.377759	1.2:-75920867	None:intergenic
TATTGTGCAATAGCGTTATT+GGG	0.380688	1.2:+75920696	MS.gene34444:CDS
GTGGCTGAATTACAAGTTAG+AGG	0.410773	1.2:+75920753	MS.gene34444:CDS
AGACCAGGGAAAACATCATC+TGG	0.456913	1.2:-75920903	None:intergenic
TCATCACACCGACCAATACT+AGG	0.469317	1.2:+75920937	MS.gene34444:CDS
GGAAAACATCATCTGGATCA+TGG	0.481353	1.2:-75920896	None:intergenic
GATTCCTCTCTACAATAAAC+GGG	0.500635	1.2:-75920866	None:intergenic
CAAGTTAGAGGGAATAGTCT+TGG	0.505805	1.2:+75920765	MS.gene34444:CDS
AACAATTTCCTAGTATTGGT+CGG	0.516529	1.2:-75920945	None:intergenic
TCATCTGGATCATGGATATG+TGG	0.554352	1.2:-75920888	None:intergenic
GAGATATATGTTGTGGGAGA+AGG	0.558851	1.2:+75920807	MS.gene34444:CDS
CACAACATGGGTTCCTCTAG+AGG	0.559347	1.2:+75920657	None:intergenic
CTAGAGGTGATTCAGCATCA+TGG	0.560521	1.2:+75920673	MS.gene34444:CDS
ATGATTCTGATAACAAGACC+AGG	0.590651	1.2:-75920918	None:intergenic
TGGCTGAATTACAAGTTAGA+GGG	0.596295	1.2:+75920754	MS.gene34444:CDS
TGTGATGAGATATATGTTGT+GGG	0.600497	1.2:+75920801	MS.gene34444:CDS
TGATGCTGAATCACCTCTAG+AGG	0.613640	1.2:-75920670	None:intergenic
GTGACCCGTTTATTGTAGAG+AGG	0.616188	1.2:+75920862	MS.gene34444:CDS
ATGTGATGAGATATATGTTG+TGG	0.646471	1.2:+75920800	MS.gene34444:CDS
ATCAATAAGAGAAAGTACTG+TGG	0.691350	1.2:+75920734	MS.gene34444:CDS
TGATTCTGATAACAAGACCA+GGG	0.737080	1.2:-75920917	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTATTGGGCATGATATTATT+GGG	+	chr1.2:75920711-75920730	MS.gene34444:CDS	25.0%
	AACACAATCAGTGAAAAATG+TGG	+	chr1.2:75920840-75920859	MS.gene34444:CDS	30.0%
	ATGTGATGAGATATATGTTG+TGG	+	chr1.2:75920800-75920819	MS.gene34444:CDS	30.0%
	CTTCAACAATTTCCTAGTAT+TGG	-	chr1.2:75920952-75920971	None:intergenic	30.0%
	GTTATTGGGCATGATATTAT+TGG	+	chr1.2:75920710-75920729	MS.gene34444:CDS	30.0%
	TATTGTGCAATAGCGTTATT+GGG	+	chr1.2:75920696-75920715	MS.gene34444:CDS	30.0%
	TGTGATGAGATATATGTTGT+GGG	+	chr1.2:75920801-75920820	MS.gene34444:CDS	30.0%
!!	AACAATTTCCTAGTATTGGT+CGG	-	chr1.2:75920948-75920967	None:intergenic	30.0%
!!	ATCAATAAGAGAAAGTACTG+TGG	+	chr1.2:75920734-75920753	MS.gene34444:CDS	30.0%
	ATGATTCTGATAACAAGACC+AGG	-	chr1.2:75920921-75920940	None:intergenic	35.0%
	GATTCCTCTCTACAATAAAC+GGG	-	chr1.2:75920869-75920888	None:intergenic	35.0%
	GGATTCCTCTCTACAATAAA+CGG	-	chr1.2:75920870-75920889	None:intergenic	35.0%
	GTATTGTGCAATAGCGTTAT+TGG	+	chr1.2:75920695-75920714	MS.gene34444:CDS	35.0%
	TGATTCTGATAACAAGACCA+GGG	-	chr1.2:75920920-75920939	None:intergenic	35.0%
	TGGCTGAATTACAAGTTAGA+GGG	+	chr1.2:75920754-75920773	MS.gene34444:CDS	35.0%
	CAAGTTAGAGGGAATAGTCT+TGG	+	chr1.2:75920765-75920784	MS.gene34444:CDS	40.0%
	GAGATATATGTTGTGGGAGA+AGG	+	chr1.2:75920807-75920826	MS.gene34444:CDS	40.0%
	GGAAAACATCATCTGGATCA+TGG	-	chr1.2:75920899-75920918	None:intergenic	40.0%
	GTGGCTGAATTACAAGTTAG+AGG	+	chr1.2:75920753-75920772	MS.gene34444:CDS	40.0%
	TCATCTGGATCATGGATATG+TGG	-	chr1.2:75920891-75920910	None:intergenic	40.0%
	AGACCAGGGAAAACATCATC+TGG	-	chr1.2:75920906-75920925	None:intergenic	45.0%
	CTAGAGGTGATTCAGCATCA+TGG	+	chr1.2:75920673-75920692	MS.gene34444:CDS	45.0%
	GTGACCCGTTTATTGTAGAG+AGG	+	chr1.2:75920862-75920881	MS.gene34444:CDS	45.0%
	TCATCACACCGACCAATACT+AGG	+	chr1.2:75920937-75920956	MS.gene34444:CDS	45.0%
	TGATGCTGAATCACCTCTAG+AGG	-	chr1.2:75920673-75920692	None:intergenic	45.0%
!	GATCCAGATGATGTTTTCCC+TGG	+	chr1.2:75920900-75920919	MS.gene34444:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	75920663	75920977	75920663	ID=MS.gene34444
chr1.2	mRNA	75920663	75920977	75920663	ID=MS.gene34444.t1;Parent=MS.gene34444
chr1.2	exon	75920663	75920977	75920663	ID=MS.gene34444.t1.exon1;Parent=MS.gene34444.t1
chr1.2	CDS	75920663	75920977	75920663	ID=cds.MS.gene34444.t1;Parent=MS.gene34444.t1

Gene Sequence	>MS.gene34444 ATGGGTTCCTCTAGAGGTGATTCAGCATCATGGTATTGTGCAATAGCGTTATTGGGCATGATATTATTGGGATCAATAAGAGAAAGTACTGTGGCTGAATTACAAGTTAGAGGGAATAGTCTTGGTGATCACAAAGCATGTGATGAGATATATGTTGTGGGAGAAGGAGAAACACTCAACACAATCAGTGAAAAATGTGGTGACCCGTTTATTGTAGAGAGGAATCCACATATCCATGATCCAGATGATGTTTTCCCTGGTCTTGTTATCAGAATCATCACACCGACCAATACTAGGAAATTGTTGAAGAGCTAG
Protein sequence	>MS.gene34444.t1 MGSSRGDSASWYCAIALLGMILLGSIRESTVAELQVRGNSLGDHKACDEIYVVGEGETLNTISEKCGDPFIVERNPHIHDPDDVFPGLVIRIITPTNTRKLLKS