AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88351


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88351.t1 MTR_5g049230 99.603 252 1 0 1 252 47 298 0.0 509
MS.gene88351.t1 MTR_5g049240 86.508 252 34 0 1 252 2 253 1.04e-160 449
MS.gene88351.t1 MTR_5g049240 86.508 252 34 0 1 252 47 298 1.47e-159 447
MS.gene88351.t1 MTR_8g069850 76.984 252 58 0 1 252 47 298 3.06e-147 414
MS.gene88351.t1 MTR_4g086150 44.017 234 124 2 19 252 61 287 2.54e-64 204
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88351.t1 AT1G13580 69.841 252 76 0 1 252 47 298 1.03e-130 372
MS.gene88351.t1 AT1G13580 69.841 252 76 0 1 252 47 298 1.03e-130 372
MS.gene88351.t1 AT3G25540 63.922 255 88 2 1 252 47 300 2.49e-118 340
MS.gene88351.t1 AT3G25540 63.922 255 88 2 1 252 47 300 2.49e-118 340
MS.gene88351.t1 AT1G26200 56.870 262 97 3 1 252 46 301 3.54e-104 305
MS.gene88351.t1 AT1G26200 54.444 270 102 3 1 252 46 312 2.67e-101 298
MS.gene88351.t1 AT1G13580 71.354 192 55 0 1 192 47 238 1.34e-99 290
MS.gene88351.t1 AT3G25540 64.615 195 66 1 1 192 47 241 2.03e-90 267
MS.gene88351.t1 AT1G26200 60.309 194 74 1 1 194 46 236 6.60e-82 245
MS.gene88351.t1 AT3G19260 45.528 246 127 2 7 252 51 289 9.46e-73 224

Find 37 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAAGATTAACAAATTTA+AGG 0.125028 5.3:+40933940 MS.gene88351:CDS
CCATACTTGCTTTAATATTC+TGG 0.136312 5.3:+40934247 MS.gene88351:CDS
TGCTTGTTCTTAACATTTAT+TGG 0.179322 5.3:+40935413 MS.gene88351:CDS
TGATGCTACTGATGTGTTTC+TGG 0.182167 5.3:+40934558 MS.gene88351:CDS
GTGTCGTTTACTACCCATTT+TGG 0.238501 5.3:+40934668 MS.gene88351:CDS
CCAGAATATTAAAGCAAGTA+TGG 0.250823 5.3:-40934247 None:intergenic
ACTGGTTGCCACCTTGAAAT+TGG 0.253838 5.3:+40935330 MS.gene88351:CDS
ACACTCAGGTTTGTTCGAAT+TGG 0.325304 5.3:+40934514 MS.gene88351:intron
GAAACAAGACGCTCTGACTT+TGG 0.337924 5.3:+40934270 MS.gene88351:CDS
GTACTTCGAAGAACCCAAAA+TGG 0.344972 5.3:-40934682 None:intergenic
CTGATTCTTCGGATGCTTGT+TGG 0.351437 5.3:+40935442 MS.gene88351:CDS
CCTTGAAATTGGAAAATCAT+TGG 0.362965 5.3:+40935341 MS.gene88351:CDS
AAACAAGACGCTCTGACTTT+GGG 0.369876 5.3:+40934271 MS.gene88351:CDS
CATACTTGCTTTAATATTCT+GGG 0.380439 5.3:+40934248 MS.gene88351:CDS
TTGGAAAATCATTGGGTGAA+TGG 0.396407 5.3:+40935349 MS.gene88351:CDS
ACTGATGTGTTTCTGGAGAT+AGG 0.414778 5.3:+40934565 MS.gene88351:CDS
CTTGAAATTGGAAAATCATT+GGG 0.421846 5.3:+40935342 MS.gene88351:CDS
ATTGGTGGGTGCTGATTCTT+CGG 0.444061 5.3:+40935431 MS.gene88351:CDS
TCTGGCCATCTCTGATTCCC+TGG 0.444917 5.3:-40934067 None:intergenic
TCTTTGTTGCAGCTATGAAC+TGG 0.456363 5.3:+40935312 MS.gene88351:intron
CTGTAACATACGACGAGCCT+TGG 0.465040 5.3:+40934014 MS.gene88351:CDS
CTGATGTGTTTCTGGAGATA+GGG 0.490323 5.3:+40934566 MS.gene88351:CDS
TTTGGGTTCTTCGAAGTACA+AGG 0.496763 5.3:+40934686 MS.gene88351:CDS
TTGTTCTTAACATTTATTGG+TGG 0.510641 5.3:+40935416 MS.gene88351:CDS
GTTAGTGAAGATATACGATC+TGG 0.513493 5.3:+40935487 MS.gene88351:CDS
GTAGTAAACGACACGTAGTA+TGG 0.517462 5.3:-40934659 None:intergenic
GGGCTGTATATGTATGGTGC+TGG 0.528738 5.3:+40934210 MS.gene88351:CDS
TGTTCTTAACATTTATTGGT+GGG 0.530572 5.3:+40935417 MS.gene88351:CDS
TTGAAAGGGCTGTATATGTA+TGG 0.545225 5.3:+40934204 MS.gene88351:CDS
TACTTCGAAGAACCCAAAAT+GGG 0.551224 5.3:-40934681 None:intergenic
TTGGGCCAGGGAATCAGAGA+TGG 0.559128 5.3:+40934062 MS.gene88351:CDS
GTTGGTCAAATTCGAGCAAG+AGG 0.587622 5.3:+40935460 MS.gene88351:CDS
TTGGTCAAATTCGAGCAAGA+GGG 0.591303 5.3:+40935461 MS.gene88351:CDS
ATGTGTAGAAATTGGCCAGA+CGG 0.612314 5.3:+40933864 None:intergenic
AAATGTAGGATAACACAATG+AGG 0.666787 5.3:-40934326 None:intergenic
GGGAAAATGTCCAAATACAG+CGG 0.695232 5.3:+40934586 MS.gene88351:CDS
ACAAATTTAAGGAATCAGCG+TGG 0.724000 5.3:+40933951 MS.gene88351:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAGATTAACAAATTTA+AGG + chr5.3:40933940-40933959 MS.gene88351:CDS 15.0%
!! AATAAATTACTCAGTTTATA+CGG + chr5.3:40935254-40935273 MS.gene88351:intron 15.0%
!! ATATCCTTTAAATTTACATT+TGG - chr5.3:40934823-40934842 None:intergenic 15.0%
!! ATTGAAAAGTTAAGTAAAAT+AGG - chr5.3:40934494-40934513 None:intergenic 15.0%
!! TATCCTTTAAATTTACATTT+GGG - chr5.3:40934822-40934841 None:intergenic 15.0%
!!! TACTGATACAAAATATTTTT+GGG + chr5.3:40934039-40934058 MS.gene88351:CDS 15.0%
!!! TTACTGATACAAAATATTTT+TGG + chr5.3:40934038-40934057 MS.gene88351:CDS 15.0%
!! AATCCCAAATGTAAATTTAA+AGG + chr5.3:40934816-40934835 MS.gene88351:intron 20.0%
!! TATATGAGTAAAGTATAAAG+TGG + chr5.3:40935121-40935140 MS.gene88351:intron 20.0%
!! TCTTGTAAAAAAGTAATCAT+TGG + chr5.3:40934866-40934885 MS.gene88351:intron 20.0%
!! TTCAATCTGAAAATTGTAAA+TGG - chr5.3:40934183-40934202 None:intergenic 20.0%
!! TTTATACTTTACTCATATAG+AGG - chr5.3:40935120-40935139 None:intergenic 20.0%
!! TTTGTTAAAATTTCAACTCT+TGG - chr5.3:40935152-40935171 None:intergenic 20.0%
!!! ATACAAAATATTTTTGGGTT+GGG + chr5.3:40934044-40934063 MS.gene88351:CDS 20.0%
!!! ATTTTCAGATTGAAATTGAA+AGG + chr5.3:40934189-40934208 MS.gene88351:intron 20.0%
!!! GAAATGTGTTTATTTTCTAT+CGG + chr5.3:40933973-40933992 MS.gene88351:CDS 20.0%
!!! TTTTCAGATTGAAATTGAAA+GGG + chr5.3:40934190-40934209 MS.gene88351:intron 20.0%
!!! TTTTTTTAACTTCCACTAAA+TGG - chr5.3:40934798-40934817 None:intergenic 20.0%
! AAAAAGTAATCATTGGTGAA+TGG + chr5.3:40934873-40934892 MS.gene88351:intron 25.0%
! AAAATAGGAAATTGAACTAG+TGG - chr5.3:40934479-40934498 None:intergenic 25.0%
! ACAACTGACAAATAACAATT+GGG - chr5.3:40934125-40934144 None:intergenic 25.0%
! CATACTTGCTTTAATATTCT+GGG + chr5.3:40934248-40934267 MS.gene88351:CDS 25.0%
! CTAGGTGAATTATATAATGT+CGG + chr5.3:40934766-40934785 MS.gene88351:intron 25.0%
! CTTGAAATTGGAAAATCATT+GGG + chr5.3:40935342-40935361 MS.gene88351:CDS 25.0%
! TGCTTGTTCTTAACATTTAT+TGG + chr5.3:40935413-40935432 MS.gene88351:CDS 25.0%
! TGTTCTTAACATTTATTGGT+GGG + chr5.3:40935417-40935436 MS.gene88351:CDS 25.0%
! TTGTTCTTAACATTTATTGG+TGG + chr5.3:40935416-40935435 MS.gene88351:CDS 25.0%
!! TATTTTGTATCAGTAAACCA+AGG - chr5.3:40934034-40934053 None:intergenic 25.0%
!! TCTTACTTAATCTTTTGATC+TGG - chr5.3:40934088-40934107 None:intergenic 25.0%
!! TTGTGTTATCCTACATTTTT+AGG + chr5.3:40934331-40934350 MS.gene88351:CDS 25.0%
!!! GATACAAAATATTTTTGGGT+TGG + chr5.3:40934043-40934062 MS.gene88351:CDS 25.0%
AAATGTAGGATAACACAATG+AGG - chr5.3:40934329-40934348 None:intergenic 30.0%
CCAGAATATTAAAGCAAGTA+TGG - chr5.3:40934250-40934269 None:intergenic 30.0%
CCATACTTGCTTTAATATTC+TGG + chr5.3:40934247-40934266 MS.gene88351:CDS 30.0%
CCTTGAAATTGGAAAATCAT+TGG + chr5.3:40935341-40935360 MS.gene88351:CDS 30.0%
CTAAACTCTTAACAAAACAG+AGG + chr5.3:40934923-40934942 MS.gene88351:intron 30.0%
GAAATCACACCTAAAAATGT+AGG - chr5.3:40934343-40934362 None:intergenic 30.0%
GACAACTGACAAATAACAAT+TGG - chr5.3:40934126-40934145 None:intergenic 30.0%
! ATGATTTTCCAATTTCAAGG+TGG - chr5.3:40935341-40935360 None:intergenic 30.0%
! CCAATGATTTTCCAATTTCA+AGG - chr5.3:40935344-40935363 None:intergenic 30.0%
! CTTAACTTTTCAATACACTC+AGG + chr5.3:40934500-40934519 MS.gene88351:intron 30.0%
! CTTTACCTTTAGGAAATTGT+CGG + chr5.3:40934376-40934395 MS.gene88351:intron 30.0%
!! CTCTGTTTTGTTAAGAGTTT+AGG - chr5.3:40934925-40934944 None:intergenic 30.0%
!!! ATATGTTAGCTTTTCAACAG+TGG + chr5.3:40935072-40935091 MS.gene88351:intron 30.0%
!!! GGCTGTGCAATTTAATTTTT+TGG + chr5.3:40934944-40934963 MS.gene88351:intron 30.0%
AATCATTGGTGAATGGTGAT+AGG + chr5.3:40934880-40934899 MS.gene88351:intron 35.0%
ACAAATTTAAGGAATCAGCG+TGG + chr5.3:40933951-40933970 MS.gene88351:CDS 35.0%
ACCTAGATTAGCAACTACAA+TGG - chr5.3:40934752-40934771 None:intergenic 35.0%
CAGTTGTTTAATCCATCATG+AGG - chr5.3:40935018-40935037 None:intergenic 35.0%
CGACAATTTCCTAAAGGTAA+AGG - chr5.3:40934378-40934397 None:intergenic 35.0%
GGTGAATTATATAATGTCGG+CGG + chr5.3:40934769-40934788 MS.gene88351:intron 35.0%
GTCTAAATGCCTTTACCTTT+AGG + chr5.3:40934366-40934385 MS.gene88351:intron 35.0%
TACAAAAAGTGACCTCATGA+TGG + chr5.3:40935003-40935022 MS.gene88351:intron 35.0%
TACTTCGAAGAACCCAAAAT+GGG - chr5.3:40934684-40934703 None:intergenic 35.0%
TTGAAAGGGCTGTATATGTA+TGG + chr5.3:40934204-40934223 MS.gene88351:CDS 35.0%
TTGGAAAATCATTGGGTGAA+TGG + chr5.3:40935349-40935368 MS.gene88351:CDS 35.0%
!! AGACGGTTCATTTTTGGAAA+GGG + chr5.3:40933881-40933900 MS.gene88351:CDS 35.0%
!! GCAACTGAGGGAATTTTAAT+AGG - chr5.3:40934730-40934749 None:intergenic 35.0%
!! TGTCGTTTACTACCCATTTT+GGG + chr5.3:40934669-40934688 MS.gene88351:CDS 35.0%
AAACAAGACGCTCTGACTTT+GGG + chr5.3:40934271-40934290 MS.gene88351:CDS 40.0%
AGACACCGACAATTTCCTAA+AGG - chr5.3:40934384-40934403 None:intergenic 40.0%
CTTTCCAAAAATGAACCGTC+TGG - chr5.3:40933882-40933901 None:intergenic 40.0%
GCCATTGTAGTTGCTAATCT+AGG + chr5.3:40934748-40934767 MS.gene88351:intron 40.0%
GGGAAAATGTCCAAATACAG+CGG + chr5.3:40934586-40934605 MS.gene88351:CDS 40.0%
GTACTTCGAAGAACCCAAAA+TGG - chr5.3:40934685-40934704 None:intergenic 40.0%
GTAGTAAACGACACGTAGTA+TGG - chr5.3:40934662-40934681 None:intergenic 40.0%
TCTTTGTTGCAGCTATGAAC+TGG + chr5.3:40935312-40935331 MS.gene88351:intron 40.0%
TGATGCTACTGATGTGTTTC+TGG + chr5.3:40934558-40934577 MS.gene88351:CDS 40.0%
TTGGTCAAATTCGAGCAAGA+GGG + chr5.3:40935461-40935480 MS.gene88351:CDS 40.0%
TTGGTGAATGGTGATAGGAT+AGG + chr5.3:40934885-40934904 MS.gene88351:intron 40.0%
! ACACTCAGGTTTGTTCGAAT+TGG + chr5.3:40934514-40934533 MS.gene88351:intron 40.0%
! TTTGGGTTCTTCGAAGTACA+AGG + chr5.3:40934686-40934705 MS.gene88351:CDS 40.0%
!! ACTGATGTGTTTCTGGAGAT+AGG + chr5.3:40934565-40934584 MS.gene88351:CDS 40.0%
!! CAGACGGTTCATTTTTGGAA+AGG + chr5.3:40933880-40933899 MS.gene88351:CDS 40.0%
!! CTGATGTGTTTCTGGAGATA+GGG + chr5.3:40934566-40934585 MS.gene88351:CDS 40.0%
!! TTTTTCAGCACCGCTGTATT+TGG - chr5.3:40934599-40934618 None:intergenic 40.0%
!!! AAATATTTTTGGGTTGGGCC+AGG + chr5.3:40934049-40934068 MS.gene88351:CDS 40.0%
!!! AATATTTTTGGGTTGGGCCA+GGG + chr5.3:40934050-40934069 MS.gene88351:CDS 40.0%
!!! GTGTCGTTTACTACCCATTT+TGG + chr5.3:40934668-40934687 MS.gene88351:CDS 40.0%
ACTGGTTGCCACCTTGAAAT+TGG + chr5.3:40935330-40935349 MS.gene88351:CDS 45.0%
AGCAACTACAATGGCAACTG+AGG - chr5.3:40934743-40934762 None:intergenic 45.0%
CTGATTCTTCGGATGCTTGT+TGG + chr5.3:40935442-40935461 MS.gene88351:CDS 45.0%
GAAACAAGACGCTCTGACTT+TGG + chr5.3:40934270-40934289 MS.gene88351:CDS 45.0%
GCAACTACAATGGCAACTGA+GGG - chr5.3:40934742-40934761 None:intergenic 45.0%
GTTGGTCAAATTCGAGCAAG+AGG + chr5.3:40935460-40935479 MS.gene88351:CDS 45.0%
! GGAAATTGTCGGTGTCTCTA+CGG + chr5.3:40934387-40934406 MS.gene88351:intron 45.0%
! TTGGCCAGACGGTTCATTTT+TGG + chr5.3:40933875-40933894 MS.gene88351:CDS 45.0%
!! ATTGGTGGGTGCTGATTCTT+CGG + chr5.3:40935431-40935450 MS.gene88351:CDS 45.0%
CTGTAACATACGACGAGCCT+TGG + chr5.3:40934014-40934033 MS.gene88351:CDS 50.0%
GGGCTGTATATGTATGGTGC+TGG + chr5.3:40934210-40934229 MS.gene88351:CDS 50.0%
TGTCGGCGGTAACCATTTAG+TGG + chr5.3:40934783-40934802 MS.gene88351:intron 50.0%
! GTCGGTGTCTCTACGGAAAT+TGG + chr5.3:40934394-40934413 MS.gene88351:intron 50.0%
TCTGGCCATCTCTGATTCCC+TGG - chr5.3:40934070-40934089 None:intergenic 55.0%
TTGGGCCAGGGAATCAGAGA+TGG + chr5.3:40934062-40934081 MS.gene88351:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 40933872 40935508 40933872 ID=MS.gene88351
chr5.3 mRNA 40933872 40935508 40933872 ID=MS.gene88351.t1;Parent=MS.gene88351
chr5.3 exon 40933872 40934101 40933872 ID=MS.gene88351.t1.exon1;Parent=MS.gene88351.t1
chr5.3 CDS 40933872 40934101 40933872 ID=cds.MS.gene88351.t1;Parent=MS.gene88351.t1
chr5.3 exon 40934197 40934352 40934197 ID=MS.gene88351.t1.exon2;Parent=MS.gene88351.t1
chr5.3 CDS 40934197 40934352 40934197 ID=cds.MS.gene88351.t1;Parent=MS.gene88351.t1
chr5.3 exon 40934522 40934707 40934522 ID=MS.gene88351.t1.exon3;Parent=MS.gene88351.t1
chr5.3 CDS 40934522 40934707 40934522 ID=cds.MS.gene88351.t1;Parent=MS.gene88351.t1
chr5.3 exon 40935324 40935508 40935324 ID=MS.gene88351.t1.exon4;Parent=MS.gene88351.t1
chr5.3 CDS 40935324 40935508 40935324 ID=cds.MS.gene88351.t1;Parent=MS.gene88351.t1
Gene Sequence

>MS.gene88351

AAATTGGCCAGACGGTTCATTTTTGGAAAGGGACATGAAGTGCTTGATTTTCATACAGATGATAGTAGAAAGAAGATTAACAAATTTAAGGAATCAGCGTGGAAATGTGTTTATTTTCTATCGGCAGAAATTTTTGCGTTGTCTGTAACATACGACGAGCCTTGGTTTACTGATACAAAATATTTTTGGGTTGGGCCAGGGAATCAGAGATGGCCAGATCAAAAGATTAAGTAAGATTTTTATTTACATGCCCAATTGTTATTTGTCAGTTGTCATTTCAAGTTAGTAGTTAACTATCGATTGAGTAACCATTTACAATTTTCAGATTGAAATTGAAAGGGCTGTATATGTATGGTGCTGGATTTTATTCATACTCCATACTTGCTTTAATATTCTGGGAAACAAGACGCTCTGACTTTGGGATTTCTATGACACATCATGTTGCTTCTCTCAGCCTCATTGTGTTATCCTACATTTTTAGGTGTGATTTCATTGTCTAAATGCCTTTACCTTTAGGAAATTGTCGGTGTCTCTACGGAAATTGGTTTTAAGAGCACATTTTGTACTTAATGTCTGCACTAATTCTATTTTATTTAAATTTACTCCACTAGTTCAATTTCCTATTTTACTTAACTTTTCAATACACTCAGGTTTGTTCGAATTGGATCAGTTGTTTTAGCTCTTCATGATGCTACTGATGTGTTTCTGGAGATAGGGAAAATGTCCAAATACAGCGGTGCTGAAAAAATCGCTAGCTTTGCATTTGTTCTGTTTGTTTTATCTTTCACCATACTACGTGTCGTTTACTACCCATTTTGGGTTCTTCGAAGTACAAGGTTTGTTTTCTTTCTTTCACCTATTAAAATTCCCTCAGTTGCCATTGTAGTTGCTAATCTAGGTGAATTATATAATGTCGGCGGTAACCATTTAGTGGAAGTTAAAAAAATCCCAAATGTAAATTTAAAGGATATGATCAGTTTGCTGCTCTTCTTAATCTTGTAAAAAAGTAATCATTGGTGAATGGTGATAGGATAGGTACACTTTTTTTCTCCTAAACTCTTAACAAAACAGAGGCTGTGCAATTTAATTTTTTGGATTTTACTTCTAAAGTGAGCAATTCTCTTCAGACGATACAAAAAGTGACCTCATGATGGATTAAACAACTGTTTATATTGCGTTTATATGCATAACAAATCATATATATGTTAGCTTTTCAACAGTGGATTTTCTCATCAATAGCTGACACCTCTATATGAGTAAAGTATAAAGTGGAAAAACCAAGAGTTGAAATTTTAACAAACTTGTAATTTGTCATGATTTATCTAGAATATTCATCTTTGACTTGTTAATTAATAATTGATAATACTCATCACATAGTCTAAAATAAATTACTCAGTTTATACGGACATATACTTTTATTTCTCTATTTAATTGTTTTATTCTTTGTTGCAGCTATGAACTGGTTGCCACCTTGAAATTGGAAAATCATTGGGTGAATGGTTCTATACATTATTATGTATTCAATAGTCTTCTATTCTGCTTGCTTGTTCTTAACATTTATTGGTGGGTGCTGATTCTTCGGATGCTTGTTGGTCAAATTCGAGCAAGAGGGAAAGTTAGTGAAGATATACGATCTG

Protein sequence

>MS.gene88351.t1

KLARRFIFGKGHEVLDFHTDDSRKKINKFKESAWKCVYFLSAEIFALSVTYDEPWFTDTKYFWVGPGNQRWPDQKIKLKLKGLYMYGAGFYSYSILALIFWETRRSDFGISMTHHVASLSLIVLSYIFRFVRIGSVVLALHDATDVFLEIGKMSKYSGAEKIASFAFVLFVLSFTILRVVYYPFWVLRSTSYELVATLKLENHWVNGSIHYYVFNSLLFCLLVLNIYWWVLILRMLVGQIRARGKVSEDIRS