AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84849

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84849.t1 MTR_1g041085 99.153 354 3 0 1 354 168 521 0.0 739
MS.gene84849.t1 MTR_1g076430 90.960 354 31 1 1 354 162 514 0.0 689
MS.gene84849.t1 MTR_2g054940 85.470 351 51 0 1 351 161 511 0.0 637
MS.gene84849.t1 MTR_7g081695 83.626 342 44 1 1 342 161 490 0.0 593
MS.gene84849.t1 MTR_4g113280 80.627 351 57 1 1 351 150 489 0.0 588
MS.gene84849.t1 MTR_7g118170 76.504 349 79 2 1 346 181 529 0.0 568
MS.gene84849.t1 MTR_4g070270 74.128 344 88 1 2 345 160 502 0.0 543
MS.gene84849.t1 MTR_1g110930 69.341 349 106 1 3 351 149 496 0.0 517
MS.gene84849.t1 MTR_5g056510 68.195 349 110 1 3 351 149 496 7.30e-180 508
MS.gene84849.t1 MTR_6g074880 66.954 348 114 1 1 347 123 470 1.40e-176 498
MS.gene84849.t1 MTR_6g032965 66.474 346 116 0 1 346 40 385 1.43e-176 496
MS.gene84849.t1 MTR_4g054415 66.763 346 114 1 1 345 80 425 7.29e-175 493
MS.gene84849.t1 MTR_4g054630 66.763 346 114 1 1 345 121 466 7.88e-175 494
MS.gene84849.t1 MTR_3g105550 64.162 346 115 1 9 345 191 536 2.91e-172 490
MS.gene84849.t1 MTR_7g118160 65.443 327 100 3 26 344 97 418 1.71e-157 449
MS.gene84849.t1 MTR_2g013740 59.878 329 128 2 9 337 179 503 6.26e-140 407
MS.gene84849.t1 MTR_2g007020 55.202 346 153 2 2 346 108 452 5.51e-138 400
MS.gene84849.t1 MTR_2g436480 53.179 346 159 2 2 345 107 451 5.59e-136 395
MS.gene84849.t1 MTR_1g102290 45.906 342 184 1 3 343 143 484 2.72e-108 325
MS.gene84849.t1 MTR_4g054260 58.984 256 87 2 91 345 87 325 1.83e-104 310
MS.gene84849.t1 MTR_3g072940 45.455 341 185 1 3 343 144 483 1.81e-102 310
MS.gene84849.t1 MTR_3g072980 45.455 341 185 1 3 343 144 483 1.87e-102 310
MS.gene84849.t1 MTR_4g062190 43.909 353 176 3 6 342 96 442 5.22e-101 306
MS.gene84849.t1 MTR_4g062270 44.868 341 181 2 6 342 96 433 9.52e-101 305
MS.gene84849.t1 MTR_2g096210 42.535 355 184 3 6 344 96 446 5.16e-100 304
MS.gene84849.t1 MTR_1g115560 41.457 357 185 3 7 342 98 451 2.19e-98 300
MS.gene84849.t1 MTR_6g051440 45.190 343 187 1 1 343 141 482 1.35e-97 298
MS.gene84849.t1 MTR_4g062170 43.840 349 181 3 6 342 96 441 4.40e-96 293
MS.gene84849.t1 MTR_4g053905 67.296 159 52 0 187 345 1 159 5.59e-76 231
MS.gene84849.t1 MTR_7g086260 59.615 52 21 0 185 236 25 76 2.60e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84849.t1 AT1G04220 84.488 361 49 3 1 354 164 524 0.0 644
MS.gene84849.t1 AT2G26640 82.906 351 60 0 1 351 159 509 0.0 627
MS.gene84849.t1 AT5G43760 84.167 360 51 3 1 354 170 529 0.0 624
MS.gene84849.t1 AT1G01120 72.965 344 92 1 3 345 183 526 0.0 551
MS.gene84849.t1 AT1G19440 74.194 341 87 1 3 343 173 512 0.0 544
MS.gene84849.t1 AT2G16280 72.141 341 94 1 3 343 169 508 0.0 535
MS.gene84849.t1 AT4G34510 70.349 344 100 2 3 345 143 485 0.0 529
MS.gene84849.t1 AT1G68530 67.335 349 113 1 3 351 150 497 1.34e-179 507
MS.gene84849.t1 AT1G25450 66.762 349 115 1 3 351 145 492 1.30e-177 502
MS.gene84849.t1 AT2G46720 64.928 345 117 2 4 345 122 465 8.91e-174 491
MS.gene84849.t1 AT2G15090 65.497 342 117 1 4 345 139 479 6.23e-166 472
MS.gene84849.t1 AT3G10280 63.188 345 116 3 4 345 122 458 8.78e-166 471
MS.gene84849.t1 AT2G26250 60.665 361 116 2 9 343 188 548 1.11e-162 466
MS.gene84849.t1 AT4G34520 64.451 346 121 2 1 345 146 490 1.20e-162 464
MS.gene84849.t1 AT4G34250 64.723 343 120 1 3 345 146 487 1.31e-162 464
MS.gene84849.t1 AT1G71160 52.339 342 161 2 2 343 107 446 1.22e-130 382
MS.gene84849.t1 AT5G49070 50.423 355 169 3 1 351 110 461 3.55e-126 370
MS.gene84849.t1 AT3G52160 53.067 326 122 2 9 334 42 336 4.28e-121 353
MS.gene84849.t1 AT3G52160 53.067 326 122 2 9 334 155 449 1.53e-119 353
MS.gene84849.t1 AT2G28630 44.286 350 181 3 7 342 97 446 3.01e-103 312
MS.gene84849.t1 AT2G28630 44.286 350 181 3 7 342 97 446 3.01e-103 312
MS.gene84849.t1 AT1G07720 44.886 352 177 3 7 342 97 447 1.81e-101 308
MS.gene84849.t1 AT1G07720 44.886 352 177 3 7 342 140 490 3.90e-101 308
MS.gene84849.t1 AT5G04530 42.857 343 187 3 6 342 98 437 8.14e-99 300
MS.gene84849.t1 AT1G68530 65.000 200 70 0 3 202 150 349 1.22e-94 287
MS.gene84849.t1 AT5G49070 47.059 204 108 0 1 204 110 313 1.74e-63 205

Find 78 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTAAGGATATTGGGATTT+TGG 0.160476 1.2:+25292894 MS.gene84849:CDS
CAAGGATTCTTCTCCTTTGC+AGG 0.220809 1.2:-25295156 None:intergenic
CCTAAAGTGTCAAAAGTTAT+TGG 0.251553 1.2:+25295213 MS.gene84849:CDS
TGCTGGTGGAAGAGCTGTTT+TGG 0.263824 1.2:+25294912 MS.gene84849:CDS
CCCATTCTAAAGAGACAATT+TGG 0.276277 1.2:-25294544 None:intergenic
TGGCAAATTGCATTTGGTTC+TGG 0.315520 1.2:+25295087 MS.gene84849:CDS
CTATTCAGCGTCATCCTTGA+TGG 0.322075 1.2:-25294979 None:intergenic
GAGTTATAACCTTGGTGGTA+TGG 0.351468 1.2:+25292999 MS.gene84849:CDS
ATAACACTAAACTGGTATTT+CGG 0.355736 1.2:+25294499 MS.gene84849:CDS
AAAACAAACATCACAACATT+AGG 0.368559 1.2:+25294760 MS.gene84849:CDS
AAAATCTTGATTTAAGTGAT+TGG 0.369920 1.2:+25294947 MS.gene84849:CDS
GAGGCGGTCGCGGGGCTTGT+TGG 0.372909 1.2:-25294587 None:intergenic
TGATTTAAGTGATTGGCATA+TGG 0.379553 1.2:+25294954 MS.gene84849:CDS
ACTACTAAGGCATATGAGTT+TGG 0.381631 1.2:-25294463 None:intergenic
GCTTCAGCCATACAAGGATT+AGG 0.382875 1.2:-25292805 None:intergenic
GCCAAATTGTCTCTTTAGAA+TGG 0.383170 1.2:+25294543 MS.gene84849:CDS
AGAACTTGGCAAATTGCATT+TGG 0.384648 1.2:+25295081 MS.gene84849:CDS
GGTGTGAAGGCTAAGGATAT+TGG 0.386847 1.2:+25292886 MS.gene84849:CDS
TAGTTCTTTGTGGTATGAAT+TGG 0.397511 1.2:+25295020 MS.gene84849:CDS
CTTCTCCGGCAACAGCCATT+AGG 0.398163 1.2:-25294732 None:intergenic
CGTCTGCACCTTTGTGCGTT+CGG 0.398737 1.2:-25294639 None:intergenic
GACACTTTAGGCACATGAAC+AGG 0.403570 1.2:-25295201 None:intergenic
GTGTGAAGGCTAAGGATATT+GGG 0.408098 1.2:+25292887 MS.gene84849:CDS
AATTTAAAATCAGGAATGTA+TGG 0.409132 1.2:-25294865 None:intergenic
GATGAGGTTCTTCAGAAAAC+TGG 0.416638 1.2:+25292865 MS.gene84849:CDS
AAAGACCTAATGGCTGTTGC+CGG 0.418979 1.2:+25294727 MS.gene84849:CDS
GGTGGTATGGGTTGTAGTGC+TGG 0.422422 1.2:+25293012 MS.gene84849:CDS
AGGATGACGCTGAATAGATT+TGG 0.423603 1.2:+25294985 MS.gene84849:CDS
TCAAAAGCTAATTTAAAATC+AGG 0.424969 1.2:-25294874 None:intergenic
TGGAGAACATAACACTAAAC+TGG 0.426255 1.2:+25294491 MS.gene84849:CDS
TTAAGTGTAATAGTGCTGTT+TGG 0.431690 1.2:+25295112 MS.gene84849:CDS
GAAGCTGAAGAGGTTATGTT+TGG 0.442456 1.2:+25292835 MS.gene84849:CDS
ATCACTCTCAAAAGACCTAA+TGG 0.453901 1.2:+25294717 MS.gene84849:CDS
CCAAATTGTCTCTTTAGAAT+GGG 0.459229 1.2:+25294544 MS.gene84849:CDS
TTTATAATGATTCACAATCA+TGG 0.459935 1.2:-25292959 None:intergenic
TTCCTTGCTTCAGCCATACA+AGG 0.461441 1.2:-25292811 None:intergenic
GTGAATCATTATAAATTGAG+AGG 0.462455 1.2:+25292967 MS.gene84849:CDS
CTGCAAAGGAGAAGAATCCT+TGG 0.462852 1.2:+25295157 MS.gene84849:CDS
AGTTATAACCTTGGTGGTAT+GGG 0.464051 1.2:+25293000 MS.gene84849:CDS
GGTCCCACCTAATCCTTGTA+TGG 0.472798 1.2:+25292798 MS.gene84849:CDS
ACACTTCAAGTAGTTCTTTG+TGG 0.479476 1.2:+25295010 MS.gene84849:CDS
GTTCTCCATGCTCACTACTA+AGG 0.483955 1.2:-25294476 None:intergenic
GGTCGCGGGGCTTGTTGGAG+AGG 0.485143 1.2:-25294582 None:intergenic
TGCACCTTTGTGCGTTCGGA+CGG 0.496713 1.2:-25294635 None:intergenic
ATCTTGAGTTATAACCTTGG+TGG 0.510802 1.2:+25292994 MS.gene84849:CDS
AATAAGAGCTGTTCCGACAT+AGG 0.515411 1.2:-25294796 None:intergenic
TGGCTTATACTGAAGCTAAA+GGG 0.515795 1.2:+25295040 MS.gene84849:CDS
TTCGGACGGTGTGGACTAGT+TGG 0.519520 1.2:-25294621 None:intergenic
TTGGCTTATACTGAAGCTAA+AGG 0.523513 1.2:+25295039 MS.gene84849:CDS
CCTTGTCAAACAGCTCCTAC+AGG 0.533062 1.2:+25293050 MS.gene84849:CDS
GAAAACTGGTGTGAAGGCTA+AGG 0.535066 1.2:+25292879 MS.gene84849:CDS
TCCGACATAGGTAGGACTAA+TGG 0.554529 1.2:-25294784 None:intergenic
TGAATCATTATAAATTGAGA+GGG 0.566439 1.2:+25292968 MS.gene84849:CDS
AGCAAGGAAAGAAGCTGAAG+AGG 0.567920 1.2:+25292825 MS.gene84849:CDS
ATATGCCTTAGTAGTGAGCA+TGG 0.583220 1.2:+25294471 MS.gene84849:CDS
TATGTTTGGTGCTATTGATG+AGG 0.584923 1.2:+25292849 MS.gene84849:CDS
CTTTGTGCGTTCGGACGGTG+TGG 0.588769 1.2:-25294630 None:intergenic
GAAGACTATTAATCCTGCAA+AGG 0.592395 1.2:+25295143 MS.gene84849:CDS
ATGGCAGAAAGAGAAGGTGT+TGG 0.592954 1.2:-25292940 None:intergenic
TCATGAATCTCATCAATCCA+AGG 0.593092 1.2:-25295174 None:intergenic
ACCATTAGTCCTACCTATGT+CGG 0.593399 1.2:+25294783 MS.gene84849:CDS
ATCCTTGTATGGCTGAAGCA+AGG 0.593578 1.2:+25292809 MS.gene84849:CDS
TCTTCAGAAAACTGGTGTGA+AGG 0.595889 1.2:+25292873 MS.gene84849:CDS
AGAGCTGTTCCGACATAGGT+AGG 0.601625 1.2:-25294792 None:intergenic
AATTGTCTCTTTAGAATGGG+AGG 0.604004 1.2:+25294547 MS.gene84849:CDS
AATATCTTGAGTTATAACCT+TGG 0.611816 1.2:+25292991 MS.gene84849:CDS
ACAATCATGGCAGAAAGAGA+AGG 0.617963 1.2:-25292946 None:intergenic
TCAGCCATACAAGGATTAGG+TGG 0.618571 1.2:-25292802 None:intergenic
TTTGATCGGAGGCGGTCGCG+GGG 0.627458 1.2:-25294595 None:intergenic
TTCTTGAAAGACGCAGCCGT+AGG 0.629235 1.2:-25294668 None:intergenic
ATTGGCATATGGAACCATCA+AGG 0.630727 1.2:+25294965 MS.gene84849:CDS
CACACCGTCCGAACGCACAA+AGG 0.645075 1.2:+25294631 MS.gene84849:CDS
CCTGTAGGAGCTGTTTGACA+AGG 0.645491 1.2:-25293050 None:intergenic
CAGCCATACAAGGATTAGGT+GGG 0.655239 1.2:-25292801 None:intergenic
CTTATACTGAAGCTAAAGGG+AGG 0.665669 1.2:+25295043 MS.gene84849:CDS
GGTGCAGACGACAAATCCTA+CGG 0.666672 1.2:+25294652 MS.gene84849:CDS
CACTACAACCCATACCACCA+AGG 0.705198 1.2:-25293008 None:intergenic
GAAGACGACACAAAGCAAGT+CGG 0.719234 1.2:+25294691 MS.gene84849:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTATATATAAATAAAA+TGG - chr1.2:25294292-25294311 None:intergenic 0.0%
!!! ATATAATTTTTATCAAATGA+TGG + chr1.2:25293951-25293970 MS.gene84849:intron 10.0%
!!! CATAATATTTTATAAACAAA+AGG - chr1.2:25294160-25294179 None:intergenic 10.0%
!!! CTTTTGTTTATAAAATATTA+TGG + chr1.2:25294158-25294177 MS.gene84849:intron 10.0%
!! ATGATAAACAATGTATATAT+AGG + chr1.2:25294435-25294454 MS.gene84849:intron 15.0%
!! TATATATACATTGTTTATCA+TGG - chr1.2:25294436-25294455 None:intergenic 15.0%
!!! GATGAAATAATATATAGTTT+TGG - chr1.2:25293107-25293126 None:intergenic 15.0%
!!! TAATATATAGTTTTGGATTA+TGG - chr1.2:25293100-25293119 None:intergenic 15.0%
!!! TTATAAAATATTATGGGTTA+TGG + chr1.2:25294165-25294184 MS.gene84849:intron 15.0%
!! AAAATCTTGATTTAAGTGAT+TGG + chr1.2:25294947-25294966 MS.gene84849:CDS 20.0%
!! AAACCTAGTTATTATTATGT+AGG - chr1.2:25293332-25293351 None:intergenic 20.0%
!! AACCTAGTTATTATTATGTA+GGG - chr1.2:25293331-25293350 None:intergenic 20.0%
!! AAGGAAAAAAATAAACTAGT+TGG - chr1.2:25294141-25294160 None:intergenic 20.0%
!! AATAAAAGATCCTAAAAATG+GGG - chr1.2:25293666-25293685 None:intergenic 20.0%
!! CAATAAAAGATCCTAAAAAT+GGG - chr1.2:25293667-25293686 None:intergenic 20.0%
!! CTTAAAAGTTAAAAGAGATT+GGG + chr1.2:25293694-25293713 MS.gene84849:intron 20.0%
!! TGAATCATTATAAATTGAGA+GGG + chr1.2:25292968-25292987 MS.gene84849:CDS 20.0%
!! TTTATAATGATTCACAATCA+TGG - chr1.2:25292962-25292981 None:intergenic 20.0%
!!! AATTTAAAATCAGGAATGTA+TGG - chr1.2:25294868-25294887 None:intergenic 20.0%
!!! TCAAAAGCTAATTTAAAATC+AGG - chr1.2:25294877-25294896 None:intergenic 20.0%
!!! TTATTGTGTAATATGGATTA+TGG - chr1.2:25294328-25294347 None:intergenic 20.0%
!!! TTTAAAATCTAAACTTGTCA+TGG - chr1.2:25294096-25294115 None:intergenic 20.0%
! AAAACAAACATCACAACATT+AGG + chr1.2:25294760-25294779 MS.gene84849:CDS 25.0%
! AAATAAATTATATCCACGAG+TGG - chr1.2:25293616-25293635 None:intergenic 25.0%
! AATATCTTGAGTTATAACCT+TGG + chr1.2:25292991-25293010 MS.gene84849:CDS 25.0%
! ACCTAGTTATTATTATGTAG+GGG - chr1.2:25293330-25293349 None:intergenic 25.0%
! ATAACACTAAACTGGTATTT+CGG + chr1.2:25294499-25294518 MS.gene84849:CDS 25.0%
! CCATATTACACAATAACAAT+TGG + chr1.2:25294332-25294351 MS.gene84849:intron 25.0%
! GCAATAAAAGATCCTAAAAA+TGG - chr1.2:25293668-25293687 None:intergenic 25.0%
! GCTTAAAAGTTAAAAGAGAT+TGG + chr1.2:25293693-25293712 MS.gene84849:intron 25.0%
! GTGAATCATTATAAATTGAG+AGG + chr1.2:25292967-25292986 MS.gene84849:CDS 25.0%
! TTAAAAAAGGTGATAGAACT+TGG + chr1.2:25295067-25295086 MS.gene84849:CDS 25.0%
!! AATTCAAGTCTATTTCTTCT+TGG + chr1.2:25293414-25293433 MS.gene84849:intron 25.0%
!! CCAATTGTTATTGTGTAATA+TGG - chr1.2:25294335-25294354 None:intergenic 25.0%
!! GGATTGAAAATAATTAGTCT+TGG - chr1.2:25293454-25293473 None:intergenic 25.0%
!!! TTAAAACATTTGATTTGCAC+AGG + chr1.2:25294111-25294130 MS.gene84849:intron 25.0%
AAATGTGATTGGATTGAACT+AGG - chr1.2:25293149-25293168 None:intergenic 30.0%
ACAACATGTCAACAAAAACA+AGG - chr1.2:25293840-25293859 None:intergenic 30.0%
ACTTTAGTAAATGCGTTCAA+AGG + chr1.2:25293559-25293578 MS.gene84849:intron 30.0%
ATCTGATCAACAAATGTGAT+TGG - chr1.2:25293160-25293179 None:intergenic 30.0%
CAACATGTCAACAAAAACAA+GGG - chr1.2:25293839-25293858 None:intergenic 30.0%
CGATATAATATACTACCTGT+AGG - chr1.2:25293068-25293087 None:intergenic 30.0%
CTACGTGCATGTTAATATTT+AGG + chr1.2:25293584-25293603 MS.gene84849:intron 30.0%
GAATTCTTGTAGTATGTAAC+AGG - chr1.2:25293399-25293418 None:intergenic 30.0%
GATCCTTATTATTTGTAGAG+TGG - chr1.2:25293478-25293497 None:intergenic 30.0%
TACAACCTGTACATTTGAAT+TGG - chr1.2:25293729-25293748 None:intergenic 30.0%
TAGTTCTTTGTGGTATGAAT+TGG + chr1.2:25295020-25295039 MS.gene84849:CDS 30.0%
TCCCCTACATAATAATAACT+AGG + chr1.2:25293326-25293345 MS.gene84849:intron 30.0%
TGATTTAAGTGATTGGCATA+TGG + chr1.2:25294954-25294973 MS.gene84849:CDS 30.0%
TTCAAATGTACAGGTTGTAA+AGG + chr1.2:25293730-25293749 MS.gene84849:intron 30.0%
TTTCATCAAACGACTGTAAT+TGG - chr1.2:25293891-25293910 None:intergenic 30.0%
! ATTTGTTGATCAGATGTGAT+TGG + chr1.2:25293165-25293184 MS.gene84849:intron 30.0%
! CAATTGGATCACAAGTTTTT+CGG + chr1.2:25294348-25294367 MS.gene84849:intron 30.0%
! CCAATAACTTTTGACACTTT+AGG - chr1.2:25295216-25295235 None:intergenic 30.0%
! CCTAAAGTGTCAAAAGTTAT+TGG + chr1.2:25295213-25295232 MS.gene84849:CDS 30.0%
! TGATAACGATTGATTGTCTA+CGG - chr1.2:25294003-25294022 None:intergenic 30.0%
! TTAAGTGTAATAGTGCTGTT+TGG + chr1.2:25295112-25295131 MS.gene84849:CDS 30.0%
!! AACATTGGAGGGTATTAATT+AGG - chr1.2:25293786-25293805 None:intergenic 30.0%
!! CCAAATTGTCTCTTTAGAAT+GGG + chr1.2:25294544-25294563 MS.gene84849:CDS 30.0%
!! GGATTTGTTGACAAGATTTA+AGG - chr1.2:25294271-25294290 None:intergenic 30.0%
AAAACAAGGGTCCATAACTA+TGG - chr1.2:25293826-25293845 None:intergenic 35.0%
AAACAAGGGTCCATAACTAT+GGG - chr1.2:25293825-25293844 None:intergenic 35.0%
ACACTTCAAGTAGTTCTTTG+TGG + chr1.2:25295010-25295029 MS.gene84849:CDS 35.0%
ACTACTAAGGCATATGAGTT+TGG - chr1.2:25294466-25294485 None:intergenic 35.0%
AGAACTTGGCAAATTGCATT+TGG + chr1.2:25295081-25295100 MS.gene84849:CDS 35.0%
ATCACTCTCAAAAGACCTAA+TGG + chr1.2:25294717-25294736 MS.gene84849:CDS 35.0%
ATCCAAGTTTCCCATAGTTA+TGG + chr1.2:25293812-25293831 MS.gene84849:intron 35.0%
ATCTTGAGTTATAACCTTGG+TGG + chr1.2:25292994-25293013 MS.gene84849:CDS 35.0%
ATGTCTCCATGATAGAGTTA+GGG - chr1.2:25293500-25293519 None:intergenic 35.0%
CCTCCACTCTACAAATAATA+AGG + chr1.2:25293472-25293491 MS.gene84849:intron 35.0%
CCTTATTATTTGTAGAGTGG+AGG - chr1.2:25293475-25293494 None:intergenic 35.0%
CTGATCCAATTCAAATGTAC+AGG + chr1.2:25293721-25293740 MS.gene84849:intron 35.0%
GAAGACTATTAATCCTGCAA+AGG + chr1.2:25295143-25295162 MS.gene84849:CDS 35.0%
GCTAAAGGGAGGATTAAAAA+AGG + chr1.2:25295054-25295073 MS.gene84849:CDS 35.0%
GTCGTTTGATGAAAATCAGA+CGG + chr1.2:25293897-25293916 MS.gene84849:intron 35.0%
TATGGTTATCCAACTAGCTA+GGG + chr1.2:25294183-25294202 MS.gene84849:intron 35.0%
TCATGAATCTCATCAATCCA+AGG - chr1.2:25295177-25295196 None:intergenic 35.0%
TGGAGAACATAACACTAAAC+TGG + chr1.2:25294491-25294510 MS.gene84849:CDS 35.0%
TGGCTTATACTGAAGCTAAA+GGG + chr1.2:25295040-25295059 MS.gene84849:CDS 35.0%
TTATGGTTATCCAACTAGCT+AGG + chr1.2:25294182-25294201 MS.gene84849:intron 35.0%
TTGGCTTATACTGAAGCTAA+AGG + chr1.2:25295039-25295058 MS.gene84849:CDS 35.0%
! AGTTATAACCTTGGTGGTAT+GGG + chr1.2:25293000-25293019 MS.gene84849:CDS 35.0%
! ATTTAGGTATTTTCCACTCG+TGG + chr1.2:25293600-25293619 MS.gene84849:intron 35.0%
! CATTTTTGTATTCATGCTGG+TGG + chr1.2:25294898-25294917 MS.gene84849:CDS 35.0%
! CCCATTCTAAAGAGACAATT+TGG - chr1.2:25294547-25294566 None:intergenic 35.0%
! GAGCATTTTTGTATTCATGC+TGG + chr1.2:25294895-25294914 MS.gene84849:CDS 35.0%
! GCCAAATTGTCTCTTTAGAA+TGG + chr1.2:25294543-25294562 MS.gene84849:CDS 35.0%
!! AATTGTCTCTTTAGAATGGG+AGG + chr1.2:25294547-25294566 MS.gene84849:CDS 35.0%
!! TATGTTTGGTGCTATTGATG+AGG + chr1.2:25292849-25292868 MS.gene84849:CDS 35.0%
!! TTGGAGGGTATTAATTAGGT+TGG - chr1.2:25293782-25293801 None:intergenic 35.0%
!!! GGACTAGTTGGTATTTTGAT+CGG - chr1.2:25294612-25294631 None:intergenic 35.0%
!!! TCGGAACAGCTCTTATTTTT+CGG + chr1.2:25294802-25294821 MS.gene84849:CDS 35.0%
AACTTGGATGCATGGAACAT+TGG - chr1.2:25293801-25293820 None:intergenic 40.0%
AAGGATCCCTAACTCTATCA+TGG + chr1.2:25293491-25293510 MS.gene84849:intron 40.0%
AATAAGAGCTGTTCCGACAT+AGG - chr1.2:25294799-25294818 None:intergenic 40.0%
AATCTTCTGCGGTCTGAATT+AGG - chr1.2:25293294-25293313 None:intergenic 40.0%
ACAATCATGGCAGAAAGAGA+AGG - chr1.2:25292949-25292968 None:intergenic 40.0%
ACCATTAGTCCTACCTATGT+CGG + chr1.2:25294783-25294802 MS.gene84849:CDS 40.0%
AGGATGACGCTGAATAGATT+TGG + chr1.2:25294985-25295004 MS.gene84849:CDS 40.0%
ATATGCCTTAGTAGTGAGCA+TGG + chr1.2:25294471-25294490 MS.gene84849:CDS 40.0%
ATTGGCATATGGAACCATCA+AGG + chr1.2:25294965-25294984 MS.gene84849:CDS 40.0%
CATGTCTCCATGATAGAGTT+AGG - chr1.2:25293501-25293520 None:intergenic 40.0%
CTAACTCTATCATGGAGACA+TGG + chr1.2:25293499-25293518 MS.gene84849:intron 40.0%
CTTATACTGAAGCTAAAGGG+AGG + chr1.2:25295043-25295062 MS.gene84849:CDS 40.0%
GAAGCTGAAGAGGTTATGTT+TGG + chr1.2:25292835-25292854 MS.gene84849:CDS 40.0%
GATGAGGTTCTTCAGAAAAC+TGG + chr1.2:25292865-25292884 MS.gene84849:CDS 40.0%
GTACAGGTTGTAAAGGTTGT+TGG + chr1.2:25293737-25293756 MS.gene84849:intron 40.0%
GTCCATAACTATGGGAAACT+TGG - chr1.2:25293817-25293836 None:intergenic 40.0%
GTGTGAAGGCTAAGGATATT+GGG + chr1.2:25292887-25292906 MS.gene84849:CDS 40.0%
TCACAACAGTTCGTTGAGAT+TGG - chr1.2:25293261-25293280 None:intergenic 40.0%
TCCAGTCAAAGAATCTTCTG+CGG - chr1.2:25293305-25293324 None:intergenic 40.0%
TCTCAACGAACTGTTGTGAT+TGG + chr1.2:25293262-25293281 MS.gene84849:intron 40.0%
TCTTCAGAAAACTGGTGTGA+AGG + chr1.2:25292873-25292892 MS.gene84849:CDS 40.0%
! AACTTGTTTCCCTAGCTAGT+TGG - chr1.2:25294195-25294214 None:intergenic 40.0%
! GAGTTATAACCTTGGTGGTA+TGG + chr1.2:25292999-25293018 MS.gene84849:CDS 40.0%
! GGCTAAGGATATTGGGATTT+TGG + chr1.2:25292894-25292913 MS.gene84849:CDS 40.0%
! TGGCAAATTGCATTTGGTTC+TGG + chr1.2:25295087-25295106 MS.gene84849:CDS 40.0%
!! AAGAGCTGTTTTGGATGAGT+TGG + chr1.2:25294921-25294940 MS.gene84849:CDS 40.0%
!!! CGGAACAGCTCTTATTTTTC+GGG + chr1.2:25294803-25294822 MS.gene84849:CDS 40.0%
!!! CTAGTTGGTATTTTGATCGG+AGG - chr1.2:25294609-25294628 None:intergenic 40.0%
!!! GCTCTTATTTTTCGGGACAT+TGG + chr1.2:25294810-25294829 MS.gene84849:CDS 40.0%
!!! GGTTGTTGGAAGTTTTTTGC+TGG + chr1.2:25293751-25293770 MS.gene84849:intron 40.0%
!!! GTTTGTTTTGAGTGCTTCTC+CGG - chr1.2:25294749-25294768 None:intergenic 40.0%
AAAGACCTAATGGCTGTTGC+CGG + chr1.2:25294727-25294746 MS.gene84849:CDS 45.0%
ACCGCAGAAGATTCTTTGAC+TGG + chr1.2:25293301-25293320 MS.gene84849:intron 45.0%
AGCAAGGAAAGAAGCTGAAG+AGG + chr1.2:25292825-25292844 MS.gene84849:CDS 45.0%
ATCCTTGTATGGCTGAAGCA+AGG + chr1.2:25292809-25292828 MS.gene84849:CDS 45.0%
ATTTATGCCAAGCCGCCTAA+GGG + chr1.2:25293632-25293651 MS.gene84849:intron 45.0%
CAAGGATTCTTCTCCTTTGC+AGG - chr1.2:25295159-25295178 None:intergenic 45.0%
CAGCCATACAAGGATTAGGT+GGG - chr1.2:25292804-25292823 None:intergenic 45.0%
CTATGGGAAACTTGGATGCA+TGG - chr1.2:25293809-25293828 None:intergenic 45.0%
CTATTCAGCGTCATCCTTGA+TGG - chr1.2:25294982-25295001 None:intergenic 45.0%
CTGCAAAGGAGAAGAATCCT+TGG + chr1.2:25295157-25295176 MS.gene84849:CDS 45.0%
GAAAACTGGTGTGAAGGCTA+AGG + chr1.2:25292879-25292898 MS.gene84849:CDS 45.0%
GAAGACGACACAAAGCAAGT+CGG + chr1.2:25294691-25294710 MS.gene84849:CDS 45.0%
GACACTTTAGGCACATGAAC+AGG - chr1.2:25295204-25295223 None:intergenic 45.0%
GCTTCAGCCATACAAGGATT+AGG - chr1.2:25292808-25292827 None:intergenic 45.0%
GGTGTGAAGGCTAAGGATAT+TGG + chr1.2:25292886-25292905 MS.gene84849:CDS 45.0%
GTTCTCCATGCTCACTACTA+AGG - chr1.2:25294479-25294498 None:intergenic 45.0%
TATTTATGCCAAGCCGCCTA+AGG + chr1.2:25293631-25293650 MS.gene84849:intron 45.0%
TCAGCCATACAAGGATTAGG+TGG - chr1.2:25292805-25292824 None:intergenic 45.0%
TCCGACATAGGTAGGACTAA+TGG - chr1.2:25294787-25294806 None:intergenic 45.0%
TGGATGCATGGAACATTGGA+GGG - chr1.2:25293797-25293816 None:intergenic 45.0%
TTCCTTGCTTCAGCCATACA+AGG - chr1.2:25292814-25292833 None:intergenic 45.0%
TTGGATGCATGGAACATTGG+AGG - chr1.2:25293798-25293817 None:intergenic 45.0%
! ATGGCAGAAAGAGAAGGTGT+TGG - chr1.2:25292943-25292962 None:intergenic 45.0%
! TCGTAGTCGTTGACTGAGAT+TGG - chr1.2:25293358-25293377 None:intergenic 45.0%
!!! GTTGGTATTTTGATCGGAGG+CGG - chr1.2:25294606-25294625 None:intergenic 45.0%
!!! TTTTGTTTATAAAATATTAT+GGG + chr1.2:25294159-25294178 MS.gene84849:intron 5.0%
AGAGCTGTTCCGACATAGGT+AGG - chr1.2:25294795-25294814 None:intergenic 50.0%
CACTACAACCCATACCACCA+AGG - chr1.2:25293011-25293030 None:intergenic 50.0%
CCTGTAGGAGCTGTTTGACA+AGG - chr1.2:25293053-25293072 None:intergenic 50.0%
CCTTGTCAAACAGCTCCTAC+AGG + chr1.2:25293050-25293069 MS.gene84849:CDS 50.0%
GGTCCCACCTAATCCTTGTA+TGG + chr1.2:25292798-25292817 MS.gene84849:CDS 50.0%
GGTGCAGACGACAAATCCTA+CGG + chr1.2:25294652-25294671 MS.gene84849:CDS 50.0%
TCAACGACTACGACTGCGAT+TGG + chr1.2:25293365-25293384 MS.gene84849:intron 50.0%
TTCTTGAAAGACGCAGCCGT+AGG - chr1.2:25294671-25294690 None:intergenic 50.0%
TTTATGCCAAGCCGCCTAAG+GGG + chr1.2:25293633-25293652 MS.gene84849:intron 50.0%
! TGCTGGTGGAAGAGCTGTTT+TGG + chr1.2:25294912-25294931 MS.gene84849:CDS 50.0%
!! GGGAATGGAGCCCCATTTTT+AGG + chr1.2:25293653-25293672 MS.gene84849:intron 50.0%
AATGGGGCTCCATTCCCCTT+AGG - chr1.2:25293650-25293669 None:intergenic 55.0%
CGTCTGCACCTTTGTGCGTT+CGG - chr1.2:25294642-25294661 None:intergenic 55.0%
CTTCTCCGGCAACAGCCATT+AGG - chr1.2:25294735-25294754 None:intergenic 55.0%
TGCACCTTTGTGCGTTCGGA+CGG - chr1.2:25294638-25294657 None:intergenic 55.0%
! GGTGGTATGGGTTGTAGTGC+TGG + chr1.2:25293012-25293031 MS.gene84849:CDS 55.0%
!! ATTTTGATCGGAGGCGGTCG+CGG - chr1.2:25294600-25294619 None:intergenic 55.0%
!! TTCGGACGGTGTGGACTAGT+TGG - chr1.2:25294624-25294643 None:intergenic 55.0%
CACACCGTCCGAACGCACAA+AGG + chr1.2:25294631-25294650 MS.gene84849:CDS 60.0%
CTTTGTGCGTTCGGACGGTG+TGG - chr1.2:25294633-25294652 None:intergenic 60.0%
TCCATTCCCCTTAGGCGGCT+TGG - chr1.2:25293642-25293661 None:intergenic 60.0%
! TTTTGATCGGAGGCGGTCGC+GGG - chr1.2:25294599-25294618 None:intergenic 60.0%
GCCAAGCCGCCTAAGGGGAA+TGG + chr1.2:25293638-25293657 MS.gene84849:intron 65.0%
GGGGCTCCATTCCCCTTAGG+CGG - chr1.2:25293647-25293666 None:intergenic 65.0%
TTTGATCGGAGGCGGTCGCG+GGG - chr1.2:25294598-25294617 None:intergenic 65.0%
!! GGTCGCGGGGCTTGTTGGAG+AGG - chr1.2:25294585-25294604 None:intergenic 70.0%
GAGGCGGTCGCGGGGCTTGT+TGG - chr1.2:25294590-25294609 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 25292793 25295242 25292793 ID=MS.gene84849
chr1.2 mRNA 25292793 25295242 25292793 ID=MS.gene84849.t1;Parent=MS.gene84849
chr1.2 exon 25292793 25293071 25292793 ID=MS.gene84849.t1.exon1;Parent=MS.gene84849.t1
chr1.2 CDS 25292793 25293071 25292793 ID=cds.MS.gene84849.t1;Parent=MS.gene84849.t1
chr1.2 exon 25294457 25295242 25294457 ID=MS.gene84849.t1.exon2;Parent=MS.gene84849.t1
chr1.2 CDS 25294457 25295242 25294457 ID=cds.MS.gene84849.t1;Parent=MS.gene84849.t1
Gene Sequence

>MS.gene84849

ATGAGGGTCCCACCTAATCCTTGTATGGCTGAAGCAAGGAAAGAAGCTGAAGAGGTTATGTTTGGTGCTATTGATGAGGTTCTTCAGAAAACTGGTGTGAAGGCTAAGGATATTGGGATTTTGGTTGTGAATTGTAGTTTGTTTAATCCAACACCTTCTCTTTCTGCCATGATTGTGAATCATTATAAATTGAGAGGGAATATCTTGAGTTATAACCTTGGTGGTATGGGTTGTAGTGCTGGTCTTATCTCTATTGACCTTGTCAAACAGCTCCTACAGGTAGTATATTATATCGCTCTATTTTCCATAATCCAAAACTATATATTATTTCATCTTTTACATGCATAGTTTAACCTAGTTCAATCCAATCACATTTGTTGATCAGATGTGATTGGACTAAACATGTTTCAAACTGCAGAAGATTCTTTGACATGAATCTTTTACATTACATATACTTAGTTTAATCCAATCTCAACGAACTGTTGTGATTGGACTAAACCTAATTCAGACCGCAGAAGATTCTTTGACTGGAATCCCCTACATAATAATAACTAGGTTTTATCCAATCTCAGTCAACGACTACGACTGCGATTGGACACTAAACCTGTTACATACTACAAGAATTCAAGTCTATTTCTTCTTGGTTTATAATATGATTCCAAGACTAATTATTTTCAATCCTCCACTCTACAAATAATAAGGATCCCTAACTCTATCATGGAGACATGGCACTTGTTGTTATGCATATATAAGTTGCTATGCAATTACTTTAGTAAATGCGTTCAAAGGTACTACGTGCATGTTAATATTTAGGTATTTTCCACTCGTGGATATAATTTATTTATGCCAAGCCGCCTAAGGGGAATGGAGCCCCATTTTTAGGATCTTTTATTGCATACTGCTTAAAAGTTAAAAGAGATTGGGACATCTGATCCAATTCAAATGTACAGGTTGTAAAGGTTGTTGGAAGTTTTTTGCTGGCTCAGCCAACCTAATTAATACCCTCCAATGTTCCATGCATCCAAGTTTCCCATAGTTATGGACCCTTGTTTTTGTTGACATGTTGTCACATAAATCACGCATTCACATAGTCAACCAATTACAGTCGTTTGATGAAAATCAGACGGCACAAATTTAAACTTCAATGATGTGCAAGTCATATAATTTTTATCAAATGATGGAGACTTTCAACTGCATAAATTCATGTCCGTAGACAATCAATCGTTATCAATGTTTTAAAAACTAAATTCGATGATTCAACTTGTTTTATATTCGAGCAGATATTTTTAAAGTAATTAAACCATGACAAGTTTAGATTTTAAAACATTTGATTTGCACAGGTTATCCAACTAGTTTATTTTTTTCCTTTTGTTTATAAAATATTATGGGTTATGGTTATCCAACTAGCTAGGGAAACAAGTTAGTTTAAATTAAAAAATTTAATAATCACTCAATTATAAGTAATAACTATGCAACCTTAAATCTTGTCAACAAATCCATTTTATTTATATATAATATAATCATATACTTCTCCATAATCCATATTACACAATAACAATTGGATCACAAGTTTTTCGGTTTATAGTATTGTATTTTTACATGACAAGTGATTCTTTTATATATCAAAGTTCAATCTCTAACCATGATAAACAATGTATATATAGGTACATCCAAACTCATATGCCTTAGTAGTGAGCATGGAGAACATAACACTAAACTGGTATTTCGGCAACGACAGATCAATGCTAGTGCCAAATTGTCTCTTTAGAATGGGAGGAGCAGCAGTTCTCCTCTCCAACAAGCCCCGCGACCGCCTCCGATCAAAATACCAACTAGTCCACACCGTCCGAACGCACAAAGGTGCAGACGACAAATCCTACGGCTGCGTCTTTCAAGAAGAAGACGACACAAAGCAAGTCGGTGTATCACTCTCAAAAGACCTAATGGCTGTTGCCGGAGAAGCACTCAAAACAAACATCACAACATTAGGACCATTAGTCCTACCTATGTCGGAACAGCTCTTATTTTTCGGGACATTGGTTGCGCGAAAAGTCTTCAAGATGAAAATCAAACCATACATTCCTGATTTTAAATTAGCTTTTGAGCATTTTTGTATTCATGCTGGTGGAAGAGCTGTTTTGGATGAGTTGGAGAAAAATCTTGATTTAAGTGATTGGCATATGGAACCATCAAGGATGACGCTGAATAGATTTGGCAACACTTCAAGTAGTTCTTTGTGGTATGAATTGGCTTATACTGAAGCTAAAGGGAGGATTAAAAAAGGTGATAGAACTTGGCAAATTGCATTTGGTTCTGGTTTTAAGTGTAATAGTGCTGTTTGGAAAGCATTGAAGACTATTAATCCTGCAAAGGAGAAGAATCCTTGGATTGATGAGATTCATGAGTTTCCTGTTCATGTGCCTAAAGTGTCAAAAGTTATTGGTTCTTAA
Protein sequence

>MS.gene84849.t1

MRVPPNPCMAEARKEAEEVMFGAIDEVLQKTGVKAKDIGILVVNCSLFNPTPSLSAMIVNHYKLRGNILSYNLGGMGCSAGLISIDLVKQLLQVHPNSYALVVSMENITLNWYFGNDRSMLVPNCLFRMGGAAVLLSNKPRDRLRSKYQLVHTVRTHKGADDKSYGCVFQEEDDTKQVGVSLSKDLMAVAGEALKTNITTLGPLVLPMSEQLLFFGTLVARKVFKMKIKPYIPDFKLAFEHFCIHAGGRAVLDELEKNLDLSDWHMEPSRMTLNRFGNTSSSSLWYELAYTEAKGRIKKGDRTWQIAFGSGFKCNSAVWKALKTINPAKEKNPWIDEIHEFPVHVPKVSKVIGS