Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83864.t1 | KEH25543.1 | 82.1 | 67 | 5 | 1 | 1 | 67 | 29 | 88 | 1.70E-21 | 111.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83864.t1 | A0A072U8W6 | 82.1 | 67 | 5 | 1 | 1 | 67 | 29 | 88 | 1.3e-21 | 111.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene83864 | 0.806608 | 7.57E-50 | -1.69E-46 |
| MS.gene04920 | MS.gene83864 | 0.805506 | 1.29E-49 | -1.69E-46 |
| MS.gene049499 | MS.gene83864 | 0.833999 | 3.86E-56 | -1.69E-46 |
| MS.gene049680 | MS.gene83864 | 0.818171 | 2.25E-52 | -1.69E-46 |
| MS.gene049711 | MS.gene83864 | 0.81902 | 1.45E-52 | -1.69E-46 |
| MS.gene049807 | MS.gene83864 | 0.827578 | 1.45E-54 | -1.69E-46 |
| MS.gene050238 | MS.gene83864 | 0.810673 | 1.03E-50 | -1.69E-46 |
| MS.gene051019 | MS.gene83864 | 0.875909 | 2.13E-68 | -1.69E-46 |
| MS.gene051121 | MS.gene83864 | 0.823312 | 1.48E-53 | -1.69E-46 |
| MS.gene051538 | MS.gene83864 | 0.815476 | 9.07E-52 | -1.69E-46 |
| MS.gene051699 | MS.gene83864 | 0.810843 | 9.42E-51 | -1.69E-46 |
| MS.gene052533 | MS.gene83864 | 0.826319 | 2.90E-54 | -1.69E-46 |
| MS.gene052579 | MS.gene83864 | 0.829667 | 4.53E-55 | -1.69E-46 |
| MS.gene052793 | MS.gene83864 | 0.844319 | 8.14E-59 | -1.69E-46 |
| MS.gene053272 | MS.gene83864 | -0.84606 | 2.75E-59 | -1.69E-46 |
| MS.gene053427 | MS.gene83864 | 0.803226 | 3.86E-49 | -1.69E-46 |
| MS.gene054142 | MS.gene83864 | 0.813585 | 2.38E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene83864 | 0.823255 | 1.53E-53 | -1.69E-46 |
| MS.gene055229 | MS.gene83864 | 0.809248 | 2.08E-50 | -1.69E-46 |
| MS.gene055339 | MS.gene83864 | 0.811854 | 5.68E-51 | -1.69E-46 |
| MS.gene055506 | MS.gene83864 | 0.80575 | 1.15E-49 | -1.69E-46 |
| MS.gene056677 | MS.gene83864 | 0.834358 | 3.14E-56 | -1.69E-46 |
| MS.gene056693 | MS.gene83864 | 0.812496 | 4.12E-51 | -1.69E-46 |
| MS.gene056699 | MS.gene83864 | 0.817399 | 3.37E-52 | -1.69E-46 |
| MS.gene05686 | MS.gene83864 | 0.832881 | 7.34E-56 | -1.69E-46 |
| MS.gene057102 | MS.gene83864 | 0.865663 | 5.01E-65 | -1.69E-46 |
| MS.gene057132 | MS.gene83864 | 0.845966 | 2.92E-59 | -1.69E-46 |
| MS.gene058781 | MS.gene83864 | 0.815718 | 8.01E-52 | -1.69E-46 |
| MS.gene05952 | MS.gene83864 | 0.810313 | 1.23E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene83864 | -0.864067 | 1.59E-64 | -1.69E-46 |
| MS.gene059900 | MS.gene83864 | -0.811388 | 7.18E-51 | -1.69E-46 |
| MS.gene060244 | MS.gene83864 | 0.800688 | 1.28E-48 | -1.69E-46 |
| MS.gene060338 | MS.gene83864 | 0.844462 | 7.45E-59 | -1.69E-46 |
| MS.gene060522 | MS.gene83864 | 0.812194 | 4.79E-51 | -1.69E-46 |
| MS.gene060906 | MS.gene83864 | 0.820559 | 6.43E-53 | -1.69E-46 |
| MS.gene060935 | MS.gene83864 | 0.810013 | 1.42E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene83864 | 0.825801 | 3.84E-54 | -1.69E-46 |
| MS.gene061100 | MS.gene83864 | 0.82287 | 1.88E-53 | -1.69E-46 |
| MS.gene061188 | MS.gene83864 | 0.888659 | 4.85E-73 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACCACTCCCAGGGGATTT+TGG | 0.070443 | 6.1:+9646526 | None:intergenic |
| GATGGGAAGAAGGGTCAATT+TGG | 0.296094 | 6.1:-9646593 | MS.gene83864:CDS |
| GGTTCATTGATGTTGGAAAA+AGG | 0.327022 | 6.1:-9646649 | MS.gene83864:CDS |
| CACCAGGCCCTTCATAATCA+TGG | 0.396641 | 6.1:+9646566 | None:intergenic |
| TTGGAACTCCATGATTATGA+AGG | 0.463143 | 6.1:-9646574 | MS.gene83864:CDS |
| ATGAAAGATTGATGGGAAGA+AGG | 0.472148 | 6.1:-9646603 | MS.gene83864:CDS |
| TGAAAGATTGATGGGAAGAA+GGG | 0.490161 | 6.1:-9646602 | MS.gene83864:CDS |
| ACATATGGATGAAAGATTGA+TGG | 0.490804 | 6.1:-9646611 | MS.gene83864:CDS |
| TTATGTTCTTTATTTGCACC+AGG | 0.490997 | 6.1:+9646550 | None:intergenic |
| GCACTAACCTTATATAAAGA+AGG | 0.507798 | 6.1:+9647570 | None:intergenic |
| CTCCATGATTATGAAGGGCC+TGG | 0.512793 | 6.1:-9646568 | MS.gene83864:CDS |
| GGAACATGGTTCATTGATGT+TGG | 0.518756 | 6.1:-9646656 | MS.gene83864:CDS |
| CATATGGATGAAAGATTGAT+GGG | 0.518975 | 6.1:-9646610 | MS.gene83864:CDS |
| GAACATAACCCAAAATCCCC+TGG | 0.526511 | 6.1:-9646535 | MS.gene83864:CDS |
| AACCCAAAATCCCCTGGGAG+TGG | 0.548305 | 6.1:-9646529 | MS.gene83864:CDS |
| TTTGCACATCAACCACTCCC+AGG | 0.556615 | 6.1:+9646517 | None:intergenic |
| TGGAACTCCATGATTATGAA+GGG | 0.574524 | 6.1:-9646573 | MS.gene83864:CDS |
| TTGCACATCAACCACTCCCA+GGG | 0.587694 | 6.1:+9646518 | None:intergenic |
| AACATAACCCAAAATCCCCT+GGG | 0.609618 | 6.1:-9646534 | MS.gene83864:CDS |
| TGCACATCAACCACTCCCAG+GGG | 0.679834 | 6.1:+9646519 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGTGAAATATATTGTATT+GGG | + | chr6.1:9647173-9647192 | None:intergenic | 15.0% |
| !! | AGAAAAATGTATAAATAAAG+AGG | + | chr6.1:9647196-9647215 | None:intergenic | 15.0% |
| !! | ATTTAGTGTTATATTGTATT+GGG | + | chr6.1:9647132-9647151 | None:intergenic | 15.0% |
| !! | TATTTAGTGTTATATTGTAT+TGG | + | chr6.1:9647133-9647152 | None:intergenic | 15.0% |
| !!! | GTTAATTAGTTAAACTTTTT+TGG | + | chr6.1:9647412-9647431 | None:intergenic | 15.0% |
| !!! | TTTACTTTTCAAATTTTTGT+TGG | - | chr6.1:9646820-9646839 | MS.gene83864:intron | 15.0% |
| !!! | TTTTTTATTCTTTTAAATCG+TGG | + | chr6.1:9646954-9646973 | None:intergenic | 15.0% |
| !! | GAAAGTGAAATATATTGTAT+TGG | + | chr6.1:9647174-9647193 | None:intergenic | 20.0% |
| !!! | AACTTTTTTGGCAAAAATTA+AGG | + | chr6.1:9647400-9647419 | None:intergenic | 20.0% |
| !!! | AGTTTTTCCTTCTTTATATA+AGG | - | chr6.1:9646556-9646575 | MS.gene83864:CDS | 20.0% |
| !!! | ATTTTTTCATCCAATATCTA+AGG | - | chr6.1:9646650-9646669 | MS.gene83864:CDS | 20.0% |
| !!! | GACACTTTTTTTTAGAAAAT+GGG | + | chr6.1:9647063-9647082 | None:intergenic | 20.0% |
| ! | TGAAAAAATGATGGTACATA+TGG | - | chr6.1:9647507-9647526 | MS.gene83864:intron | 25.0% |
| !! | AAAGTCATGGTTATGTATTT+TGG | - | chr6.1:9646625-9646644 | MS.gene83864:CDS | 25.0% |
| !! | ATCTTCACCATTCAAATTTT+TGG | + | chr6.1:9646531-9646550 | None:intergenic | 25.0% |
| !! | TCATTATTTGTGTGTTTTGT+AGG | - | chr6.1:9647456-9647475 | MS.gene83864:intron | 25.0% |
| !! | TTGAAATGACTCAGTTATTT+TGG | + | chr6.1:9647086-9647105 | None:intergenic | 25.0% |
| !!! | GGACACTTTTTTTTAGAAAA+TGG | + | chr6.1:9647064-9647083 | None:intergenic | 25.0% |
| !!! | TGAAATGACTCAGTTATTTT+GGG | + | chr6.1:9647085-9647104 | None:intergenic | 25.0% |
| ACATATGGATGAAAGATTGA+TGG | - | chr6.1:9647522-9647541 | MS.gene83864:intron | 30.0% | |
| AGAAAATGGGTCATATATTC+CGG | + | chr6.1:9647050-9647069 | None:intergenic | 30.0% | |
| CATATGGATGAAAGATTGAT+GGG | - | chr6.1:9647523-9647542 | MS.gene83864:intron | 30.0% | |
| CCAATATCTAAGGAAAAATG+AGG | - | chr6.1:9646660-9646679 | MS.gene83864:intron | 30.0% | |
| GCACTAACCTTATATAAAGA+AGG | + | chr6.1:9646566-9646585 | None:intergenic | 30.0% | |
| GGAAAAAGGTGAAAAAATGA+TGG | - | chr6.1:9647498-9647517 | MS.gene83864:intron | 30.0% | |
| ! | TTATGTTCTTTATTTGCACC+AGG | + | chr6.1:9647586-9647605 | None:intergenic | 30.0% |
| !! | CCTCATTTTTCCTTAGATAT+TGG | + | chr6.1:9646663-9646682 | None:intergenic | 30.0% |
| AACCATGACTTTGCAAGAAT+AGG | + | chr6.1:9646617-9646636 | None:intergenic | 35.0% | |
| AATCTGTGTGAAATGATCCA+AGG | - | chr6.1:9647217-9647236 | MS.gene83864:intron | 35.0% | |
| ATCTGTGTGAAATGATCCAA+GGG | - | chr6.1:9647218-9647237 | MS.gene83864:intron | 35.0% | |
| ATGAAAGATTGATGGGAAGA+AGG | - | chr6.1:9647530-9647549 | MS.gene83864:intron | 35.0% | |
| GAAAATGGGTCATATATTCC+GGG | + | chr6.1:9647049-9647068 | None:intergenic | 35.0% | |
| GGTTCATTGATGTTGGAAAA+AGG | - | chr6.1:9647484-9647503 | MS.gene83864:intron | 35.0% | |
| TGAAAGATTGATGGGAAGAA+GGG | - | chr6.1:9647531-9647550 | MS.gene83864:intron | 35.0% | |
| TGGAACTCCATGATTATGAA+GGG | - | chr6.1:9647560-9647579 | MS.gene83864:intron | 35.0% | |
| TTCCTATTCTTGCAAAGTCA+TGG | - | chr6.1:9646612-9646631 | MS.gene83864:CDS | 35.0% | |
| TTGGAACTCCATGATTATGA+AGG | - | chr6.1:9647559-9647578 | MS.gene83864:intron | 35.0% | |
| ! | TTGTGTGTTTTGTAGGAACA+TGG | - | chr6.1:9647463-9647482 | MS.gene83864:intron | 35.0% |
| AACATAACCCAAAATCCCCT+GGG | - | chr6.1:9647599-9647618 | MS.gene83864:CDS | 40.0% | |
| ATCCAAGGGGTCAGATAAAT+TGG | - | chr6.1:9647232-9647251 | MS.gene83864:intron | 40.0% | |
| CAACAATTGTGTCAAGCAGA+AGG | - | chr6.1:9646774-9646793 | MS.gene83864:intron | 40.0% | |
| GGAACATGGTTCATTGATGT+TGG | - | chr6.1:9647477-9647496 | MS.gene83864:intron | 40.0% | |
| TCCAAGGGGTCAGATAAATT+GGG | - | chr6.1:9647233-9647252 | MS.gene83864:intron | 40.0% | |
| TCTGTGTGAAATGATCCAAG+GGG | - | chr6.1:9647219-9647238 | MS.gene83864:intron | 40.0% | |
| AGGGGTCAGATAAATTGGGA+CGG | - | chr6.1:9647237-9647256 | MS.gene83864:intron | 45.0% | |
| ATGGGTCATATATTCCGGGA+CGG | + | chr6.1:9647045-9647064 | None:intergenic | 45.0% | |
| GAACATAACCCAAAATCCCC+TGG | - | chr6.1:9647598-9647617 | MS.gene83864:CDS | 45.0% | |
| GATGGGAAGAAGGGTCAATT+TGG | - | chr6.1:9647540-9647559 | MS.gene83864:intron | 45.0% | |
| GTCAGATAAATTGGGACGGA+GGG | - | chr6.1:9647241-9647260 | MS.gene83864:intron | 45.0% | |
| TCCCAATTTATCTGACCCCT+TGG | + | chr6.1:9647237-9647256 | None:intergenic | 45.0% | |
| !!! | ATTAACTTTTTAATTTATTT+TGG | - | chr6.1:9647426-9647445 | MS.gene83864:intron | 5.0% |
| CACCAGGCCCTTCATAATCA+TGG | + | chr6.1:9647570-9647589 | None:intergenic | 50.0% | |
| CTATATTACTCCCTCCGTCC+CGG | - | chr6.1:9647028-9647047 | MS.gene83864:intron | 50.0% | |
| CTCCATGATTATGAAGGGCC+TGG | - | chr6.1:9647565-9647584 | MS.gene83864:intron | 50.0% | |
| GGTCAGATAAATTGGGACGG+AGG | - | chr6.1:9647240-9647259 | MS.gene83864:intron | 50.0% | |
| GTCATATATTCCGGGACGGA+GGG | + | chr6.1:9647041-9647060 | None:intergenic | 50.0% | |
| ! | AACCACTCCCAGGGGATTTT+GGG | + | chr6.1:9647609-9647628 | None:intergenic | 50.0% |
| AACCCAAAATCCCCTGGGAG+TGG | - | chr6.1:9647604-9647623 | MS.gene83864:CDS | 55.0% | |
| GGTCATATATTCCGGGACGG+AGG | + | chr6.1:9647042-9647061 | None:intergenic | 55.0% | |
| ! | CAACCACTCCCAGGGGATTT+TGG | + | chr6.1:9647610-9647629 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 9646525 | 9647630 | 9646525 | ID=MS.gene83864 |
| chr6.1 | mRNA | 9646525 | 9647630 | 9646525 | ID=MS.gene83864.t1;Parent=MS.gene83864 |
| chr6.1 | exon | 9647578 | 9647630 | 9647578 | ID=MS.gene83864.t1.exon1;Parent=MS.gene83864.t1 |
| chr6.1 | CDS | 9647578 | 9647630 | 9647578 | ID=cds.MS.gene83864.t1;Parent=MS.gene83864.t1 |
| chr6.1 | exon | 9646525 | 9646677 | 9646525 | ID=MS.gene83864.t1.exon2;Parent=MS.gene83864.t1 |
| chr6.1 | CDS | 9646525 | 9646677 | 9646525 | ID=cds.MS.gene83864.t1;Parent=MS.gene83864.t1 |
| Gene Sequence |
| Protein sequence |
