Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38207.t1 | XP_003617956.1 | 98.2 | 503 | 9 | 0 | 1 | 503 | 63 | 565 | 3.70E-249 | 870.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38207.t1 | Q84N37 | 89.3 | 503 | 54 | 0 | 1 | 503 | 11 | 513 | 6.2e-229 | 794.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38207.t1 | G7KEP0 | 98.2 | 503 | 9 | 0 | 1 | 503 | 63 | 565 | 2.7e-249 | 870.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene38207 | 0.808346 | 3.24E-50 | -1.69E-46 |
| MS.gene049254 | MS.gene38207 | 0.804345 | 2.26E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene38207 | 0.812822 | 3.49E-51 | -1.69E-46 |
| MS.gene049499 | MS.gene38207 | 0.829394 | 5.28E-55 | -1.69E-46 |
| MS.gene049711 | MS.gene38207 | 0.813038 | 3.13E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene38207 | 0.80787 | 4.09E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene38207 | 0.809546 | 1.79E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene38207 | 0.810158 | 1.32E-50 | -1.69E-46 |
| MS.gene050562 | MS.gene38207 | 0.8009 | 1.16E-48 | -1.69E-46 |
| MS.gene050646 | MS.gene38207 | 0.842009 | 3.36E-58 | -1.69E-46 |
| MS.gene050945 | MS.gene38207 | 0.821647 | 3.61E-53 | -1.69E-46 |
| MS.gene051019 | MS.gene38207 | 0.815587 | 8.57E-52 | -1.69E-46 |
| MS.gene051154 | MS.gene38207 | 0.822731 | 2.02E-53 | -1.69E-46 |
| MS.gene051234 | MS.gene38207 | 0.80072 | 1.26E-48 | -1.69E-46 |
| MS.gene051279 | MS.gene38207 | 0.811808 | 5.82E-51 | -1.69E-46 |
| MS.gene051699 | MS.gene38207 | 0.849288 | 3.56E-60 | -1.69E-46 |
| MS.gene051744 | MS.gene38207 | 0.816864 | 4.44E-52 | -1.69E-46 |
| MS.gene051832 | MS.gene38207 | 0.804872 | 1.75E-49 | -1.69E-46 |
| MS.gene051975 | MS.gene38207 | 0.802753 | 4.83E-49 | -1.69E-46 |
| MS.gene052016 | MS.gene38207 | 0.815065 | 1.12E-51 | -1.69E-46 |
| MS.gene052118 | MS.gene38207 | 0.801881 | 7.31E-49 | -1.69E-46 |
| MS.gene052239 | MS.gene38207 | -0.802731 | 4.88E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene38207 | 0.813168 | 2.93E-51 | -1.69E-46 |
| MS.gene052533 | MS.gene38207 | 0.829359 | 5.38E-55 | -1.69E-46 |
| MS.gene052609 | MS.gene38207 | 0.80111 | 1.05E-48 | -1.69E-46 |
| MS.gene053272 | MS.gene38207 | -0.844535 | 7.12E-59 | -1.69E-46 |
| MS.gene054178 | MS.gene38207 | 0.823299 | 1.49E-53 | -1.69E-46 |
| MS.gene05423 | MS.gene38207 | 0.822189 | 2.71E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene38207 | 0.81319 | 2.90E-51 | -1.69E-46 |
| MS.gene054709 | MS.gene38207 | 0.812816 | 3.51E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene38207 | 0.858267 | 9.26E-63 | -1.69E-46 |
| MS.gene054893 | MS.gene38207 | 0.853157 | 2.88E-61 | -1.69E-46 |
| MS.gene055150 | MS.gene38207 | 0.802367 | 5.80E-49 | -1.69E-46 |
| MS.gene055229 | MS.gene38207 | 0.826132 | 3.21E-54 | -1.69E-46 |
| MS.gene055386 | MS.gene38207 | 0.803032 | 4.23E-49 | -1.69E-46 |
| MS.gene056082 | MS.gene38207 | 0.800197 | 1.61E-48 | -1.69E-46 |
| MS.gene056174 | MS.gene38207 | 0.808219 | 3.45E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene38207 | 0.814161 | 1.77E-51 | -1.69E-46 |
| MS.gene056444 | MS.gene38207 | 0.808317 | 3.29E-50 | -1.69E-46 |
| MS.gene056677 | MS.gene38207 | 0.827135 | 1.85E-54 | -1.69E-46 |
| MS.gene056699 | MS.gene38207 | 0.823896 | 1.08E-53 | -1.69E-46 |
| MS.gene05686 | MS.gene38207 | 0.860157 | 2.51E-63 | -1.69E-46 |
| MS.gene05690 | MS.gene38207 | 0.807905 | 4.02E-50 | -1.69E-46 |
| MS.gene056973 | MS.gene38207 | 0.816359 | 5.76E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene38207 | 0.881519 | 2.24E-70 | -1.69E-46 |
| MS.gene057174 | MS.gene38207 | 0.820577 | 6.37E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene38207 | 0.819494 | 1.13E-52 | -1.69E-46 |
| MS.gene058095 | MS.gene38207 | 0.801229 | 9.94E-49 | -1.69E-46 |
| MS.gene058159 | MS.gene38207 | 0.834762 | 2.49E-56 | -1.69E-46 |
| MS.gene058279 | MS.gene38207 | 0.81401 | 1.92E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene38207 | 0.824928 | 6.19E-54 | -1.69E-46 |
| MS.gene058783 | MS.gene38207 | 0.813098 | 3.04E-51 | -1.69E-46 |
| MS.gene059170 | MS.gene38207 | 0.825891 | 3.66E-54 | -1.69E-46 |
| MS.gene05952 | MS.gene38207 | 0.843204 | 1.62E-58 | -1.69E-46 |
| MS.gene059774 | MS.gene38207 | 0.820059 | 8.38E-53 | -1.69E-46 |
| MS.gene05985 | MS.gene38207 | -0.879245 | 1.46E-69 | -1.69E-46 |
| MS.gene060244 | MS.gene38207 | 0.800812 | 1.21E-48 | -1.69E-46 |
| MS.gene060259 | MS.gene38207 | 0.858132 | 1.02E-62 | -1.69E-46 |
| MS.gene060338 | MS.gene38207 | 0.809512 | 1.82E-50 | -1.69E-46 |
| MS.gene060365 | MS.gene38207 | 0.808371 | 3.20E-50 | -1.69E-46 |
| MS.gene060522 | MS.gene38207 | 0.837536 | 4.91E-57 | -1.69E-46 |
| MS.gene060697 | MS.gene38207 | 0.815304 | 9.90E-52 | -1.69E-46 |
| MS.gene060906 | MS.gene38207 | 0.802414 | 5.67E-49 | -1.69E-46 |
| MS.gene060933 | MS.gene38207 | 0.82287 | 1.88E-53 | -1.69E-46 |
| MS.gene060935 | MS.gene38207 | 0.828946 | 6.78E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene38207 | 0.841487 | 4.61E-58 | -1.69E-46 |
| MS.gene061100 | MS.gene38207 | 0.824161 | 9.38E-54 | -1.69E-46 |
| MS.gene061160 | MS.gene38207 | 0.806339 | 8.63E-50 | -1.69E-46 |
| MS.gene061188 | MS.gene38207 | 0.86434 | 1.31E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene38207 | MS.gene020889 | PPI |
| MS.gene38207 | MS.gene38052 | PPI |
| MS.gene020889 | MS.gene38207 | PPI |
| MS.gene81225 | MS.gene38207 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38207.t1 | MTR_5g097360 | 99.404 | 503 | 3 | 0 | 1 | 503 | 63 | 565 | 0.0 | 1035 |
| MS.gene38207.t1 | MTR_4g039650 | 37.363 | 455 | 268 | 10 | 30 | 482 | 785 | 1224 | 1.07e-84 | 285 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38207.t1 | AT5G48160 | 72.636 | 497 | 127 | 6 | 14 | 503 | 80 | 574 | 0.0 | 731 |
| MS.gene38207.t1 | AT5G48160 | 72.636 | 497 | 127 | 6 | 14 | 503 | 80 | 574 | 0.0 | 731 |
| MS.gene38207.t1 | AT3G07780 | 67.129 | 505 | 158 | 5 | 2 | 503 | 67 | 566 | 0.0 | 665 |
| MS.gene38207.t1 | AT3G07780 | 67.129 | 505 | 158 | 5 | 2 | 503 | 67 | 566 | 0.0 | 665 |
| MS.gene38207.t1 | AT3G07780 | 67.129 | 505 | 158 | 5 | 2 | 503 | 78 | 577 | 0.0 | 664 |
| MS.gene38207.t1 | AT3G63500 | 36.105 | 421 | 251 | 6 | 30 | 444 | 432 | 840 | 1.41e-78 | 263 |
| MS.gene38207.t1 | AT3G63500 | 36.105 | 421 | 251 | 6 | 30 | 444 | 707 | 1115 | 3.90e-77 | 263 |
| MS.gene38207.t1 | AT3G63500 | 36.105 | 421 | 251 | 6 | 30 | 444 | 707 | 1115 | 3.90e-77 | 263 |
| MS.gene38207.t1 | AT1G14740 | 34.169 | 439 | 244 | 6 | 27 | 460 | 305 | 703 | 3.73e-72 | 243 |
Find 116 sgRNAs with CRISPR-Local
Find 135 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCCTGAATTCCAGTATTTA+TGG | 0.176236 | 5.3:-78685080 | None:intergenic |
| CTAATGACTGCCACCCTTTC+CGG | 0.193878 | 5.3:+78686607 | MS.gene38207:CDS |
| GTAAAGGAGATCATGGATTT+TGG | 0.213920 | 5.3:-78686556 | None:intergenic |
| TGCCATAAATACTGGAATTC+AGG | 0.237888 | 5.3:+78685078 | MS.gene38207:CDS |
| CTTGAGTTCTTCTGCTTCTC+TGG | 0.253475 | 5.3:-78686232 | None:intergenic |
| GGCACTGATCCACTCTTTAC+AGG | 0.283245 | 5.3:-78685382 | None:intergenic |
| CATCAGCTGGAAGCTGGTTT+TGG | 0.287912 | 5.3:-78685183 | None:intergenic |
| TTAGATTGACTGTCTGCTTT+GGG | 0.315236 | 5.3:-78686588 | None:intergenic |
| ATCCTTGTTGCCATAAATAC+TGG | 0.341420 | 5.3:+78685070 | MS.gene38207:CDS |
| CTTCTGTTTCAAATAGTTGC+TGG | 0.354580 | 5.3:-78686424 | None:intergenic |
| ATCGTGGGATGAACCTCTTT+TGG | 0.374814 | 5.3:+78685563 | MS.gene38207:CDS |
| ATTAGATTGACTGTCTGCTT+TGG | 0.379288 | 5.3:-78686589 | None:intergenic |
| TGGCCTCATTAGCCTTCAAT+TGG | 0.386901 | 5.3:-78686335 | None:intergenic |
| GAGCCTCTCAGCCTCTCGTT+TGG | 0.409388 | 5.3:-78686355 | None:intergenic |
| TGCGGACAGAATCAGTCTTC+AGG | 0.422728 | 5.3:+78684832 | MS.gene38207:CDS |
| GACATTGATTCGAGCTCATC+GGG | 0.423325 | 5.3:+78685036 | MS.gene38207:CDS |
| GTATGAGAAAATCAAATTGC+AGG | 0.425425 | 5.3:+78686479 | MS.gene38207:CDS |
| TTTGTTAGTAGGATCTTTCA+TGG | 0.429043 | 5.3:+78685529 | MS.gene38207:CDS |
| GATGTGATTTGTGTTCTCAT+TGG | 0.433032 | 5.3:+78685311 | MS.gene38207:CDS |
| CACCTGAAAACCATTTCACT+TGG | 0.434640 | 5.3:-78685403 | None:intergenic |
| GGCTGATATGTTCCAATTGA+AGG | 0.436823 | 5.3:+78686323 | MS.gene38207:CDS |
| TGGATCAGTGCCAAGTGAAA+TGG | 0.456270 | 5.3:+78685393 | MS.gene38207:CDS |
| TCTTGCGAAGTCTGATAAAT+CGG | 0.461104 | 5.3:+78686389 | MS.gene38207:CDS |
| GCCATAAATACTGGAATTCA+GGG | 0.463071 | 5.3:+78685079 | MS.gene38207:CDS |
| CCAGGCTCTTTGGAGAGTCT+AGG | 0.472168 | 5.3:-78686038 | None:intergenic |
| GATCGTCACACTTCCAAAAG+AGG | 0.472222 | 5.3:-78685576 | None:intergenic |
| ATACTCATCACGGGTTTGTC+CGG | 0.472397 | 5.3:-78686626 | None:intergenic |
| TGGAAGGTTGATTGCTCCAC+AGG | 0.475183 | 5.3:+78686074 | MS.gene38207:CDS |
| TGGAAGCTAAAGCAGCATGC+AGG | 0.476217 | 5.3:+78685626 | MS.gene38207:CDS |
| TCCAACATTGTGCACCATCA+TGG | 0.480513 | 5.3:+78685476 | MS.gene38207:CDS |
| GGCCATTGTAAAGGAGATCA+TGG | 0.483195 | 5.3:-78686563 | None:intergenic |
| CAGAATTGCTGAAGTGGTGC+AGG | 0.488827 | 5.3:+78686107 | MS.gene38207:CDS |
| CGACGAATAACTGACTGATC+AGG | 0.490361 | 5.3:-78684800 | None:intergenic |
| CTTTGGGAGGGCCATTGTAA+AGG | 0.496266 | 5.3:-78686572 | None:intergenic |
| GATTGACTGTCTGCTTTGGG+AGG | 0.497767 | 5.3:-78686585 | None:intergenic |
| ATTGCAGAAGCCACTGTTGT+TGG | 0.498959 | 5.3:-78685227 | None:intergenic |
| GGACTTCGAGTTATCCTTGA+GGG | 0.499090 | 5.3:+78684935 | MS.gene38207:CDS |
| AACAATCATCAGCTGGAAGC+TGG | 0.500810 | 5.3:-78685189 | None:intergenic |
| GCAGGAGGCTATACGAAAGA+TGG | 0.504278 | 5.3:+78686125 | MS.gene38207:CDS |
| ACTTCGCAAGAGCAATCCTC+TGG | 0.508330 | 5.3:-78686377 | None:intergenic |
| GCAAATAAGTTGCTCCCGTA+TGG | 0.512263 | 5.3:-78685350 | None:intergenic |
| TCAGTCTTCAGGACATTGCT+CGG | 0.513007 | 5.3:+78684843 | MS.gene38207:CDS |
| GGACTTCAGAACTGTTAGGC+TGG | 0.513412 | 5.3:+78685440 | MS.gene38207:CDS |
| CTCACACAGATTGTGCCATA+CGG | 0.513413 | 5.3:+78685335 | MS.gene38207:CDS |
| CCTAGACTCTCCAAAGAGCC+TGG | 0.513548 | 5.3:+78686038 | MS.gene38207:CDS |
| GTAAATACATGCCGTTGGAT+TGG | 0.515809 | 5.3:+78685289 | MS.gene38207:CDS |
| GAAGGCTCGCTTAGCTGTTG+AGG | 0.516614 | 5.3:+78686182 | MS.gene38207:CDS |
| ATCACGGGTTTGTCCGGAAA+GGG | 0.518375 | 5.3:-78686620 | None:intergenic |
| GTTCCAATTGAAGGCTAATG+AGG | 0.521411 | 5.3:+78686332 | MS.gene38207:CDS |
| TTGAAGTAAATACATGCCGT+TGG | 0.522716 | 5.3:+78685284 | MS.gene38207:CDS |
| CCCATGATGGTGCACAATGT+TGG | 0.522804 | 5.3:-78685477 | None:intergenic |
| GACTTCAGAACTGTTAGGCT+GGG | 0.527791 | 5.3:+78685441 | MS.gene38207:CDS |
| GGCTGCGACAGACTAATGTT+AGG | 0.528186 | 5.3:-78685112 | None:intergenic |
| ATTGACTGTCTGCTTTGGGA+GGG | 0.528716 | 5.3:-78686584 | None:intergenic |
| AGAAGCAGAAGAACTCAAGA+TGG | 0.529192 | 5.3:+78686236 | MS.gene38207:CDS |
| ACGGGAGCAACTTATTTGCA+TGG | 0.531202 | 5.3:+78685354 | MS.gene38207:CDS |
| AGACATTGATTCGAGCTCAT+CGG | 0.534133 | 5.3:+78685035 | MS.gene38207:CDS |
| ATGAGCTCGAATCAATGTCT+TGG | 0.537389 | 5.3:-78685032 | None:intergenic |
| AGCCTGGAAAATGTAGAAAG+TGG | 0.542593 | 5.3:+78686054 | MS.gene38207:CDS |
| GTTATCCTTGAGGGAGGGAA+TGG | 0.544054 | 5.3:+78684944 | MS.gene38207:CDS |
| TTGGAAGTGTGACGATCTCA+AGG | 0.544756 | 5.3:+78685582 | MS.gene38207:CDS |
| CCAACATTGTGCACCATCAT+GGG | 0.546323 | 5.3:+78685477 | MS.gene38207:CDS |
| CAGTCTTCAGGACATTGCTC+GGG | 0.548827 | 5.3:+78684844 | MS.gene38207:CDS |
| ATTCTGTTGCATGCCTCCTG+TGG | 0.548889 | 5.3:-78686090 | None:intergenic |
| AAAATCAAGCTCCCTCATCA+AGG | 0.557639 | 5.3:-78685509 | None:intergenic |
| TCACAAGAACAATCATCAGC+TGG | 0.558649 | 5.3:-78685196 | None:intergenic |
| CGGGAGCAACTTATTTGCAT+GGG | 0.558837 | 5.3:+78685355 | MS.gene38207:CDS |
| ACAGAAGTTAAGTGAGGCTG+AGG | 0.558933 | 5.3:+78686440 | MS.gene38207:CDS |
| GGAAGCTAAAGCAGCATGCA+GGG | 0.560687 | 5.3:+78685627 | MS.gene38207:CDS |
| GCTGCGAAGAAGATGGAGAA+TGG | 0.561971 | 5.3:+78684758 | None:intergenic |
| TCACACAGATTGTGCCATAC+GGG | 0.562732 | 5.3:+78685336 | MS.gene38207:CDS |
| TCAGGTGTCAATCATGCAGT+AGG | 0.562912 | 5.3:+78685419 | MS.gene38207:CDS |
| GAATACATCAGCATTGAAGA+GGG | 0.569622 | 5.3:-78686534 | None:intergenic |
| ATTGTGCACCATCATGGGAT+GGG | 0.570954 | 5.3:+78685482 | MS.gene38207:CDS |
| CATTGTGCACCATCATGGGA+TGG | 0.578553 | 5.3:+78685481 | MS.gene38207:CDS |
| AGTAGGACTTCAGAACTGTT+AGG | 0.579004 | 5.3:+78685436 | MS.gene38207:CDS |
| GGACATTGCTCGGGAAAGAG+TGG | 0.584255 | 5.3:+78684853 | MS.gene38207:CDS |
| GGAATACATCAGCATTGAAG+AGG | 0.585828 | 5.3:-78686535 | None:intergenic |
| CAATTGGAACATATCAGCCT+CGG | 0.587516 | 5.3:-78686319 | None:intergenic |
| GCATGCCATTCCCTCCCTCA+AGG | 0.588171 | 5.3:-78684949 | None:intergenic |
| CAGAAGAACTCAAGATGGAG+AGG | 0.588684 | 5.3:+78686241 | MS.gene38207:CDS |
| CATCACGGGTTTGTCCGGAA+AGG | 0.588989 | 5.3:-78686621 | None:intergenic |
| TTGTGCACCATCATGGGATG+GGG | 0.590598 | 5.3:+78685483 | MS.gene38207:CDS |
| AAGGAATCCCCATCCCATGA+TGG | 0.593870 | 5.3:-78685490 | None:intergenic |
| GAAGATGAGAATGTTCAAGA+AGG | 0.594556 | 5.3:+78686164 | MS.gene38207:CDS |
| ATGGTAGTAAAGACCATCGT+GGG | 0.598320 | 5.3:+78685548 | MS.gene38207:CDS |
| GAGAGGCTGAGAGGCTCCAG+AGG | 0.598779 | 5.3:+78686361 | MS.gene38207:CDS |
| TTCCAGTATTTATGGCAACA+AGG | 0.603589 | 5.3:-78685072 | None:intergenic |
| AGAAATATCTCAATCAGCGA+TGG | 0.603706 | 5.3:-78685133 | None:intergenic |
| TGGAAAATGTAGAAAGTGGA+AGG | 0.603944 | 5.3:+78686058 | MS.gene38207:CDS |
| ATGCAACAGAATTGCTGAAG+TGG | 0.604458 | 5.3:+78686101 | MS.gene38207:CDS |
| ATCCATGATCTCCTTTACAA+TGG | 0.611502 | 5.3:+78686561 | MS.gene38207:CDS |
| CTTCGAGTTATCCTTGAGGG+AGG | 0.614388 | 5.3:+78684938 | MS.gene38207:CDS |
| TTCGAGTTATCCTTGAGGGA+GGG | 0.615596 | 5.3:+78684939 | MS.gene38207:CDS |
| GGCTAATGAGGCCAAACGAG+AGG | 0.618483 | 5.3:+78686344 | MS.gene38207:CDS |
| AATTGCTGAAGTGGTGCAGG+AGG | 0.618880 | 5.3:+78686110 | MS.gene38207:CDS |
| CACAGATTGAAGAACTAGAG+AGG | 0.620430 | 5.3:+78686277 | MS.gene38207:CDS |
| TATTCGTCGTGTTTCTAGTG+CGG | 0.628975 | 5.3:+78684814 | MS.gene38207:CDS |
| ACGGGTTTGTCCGGAAAGGG+TGG | 0.629535 | 5.3:-78686617 | None:intergenic |
| TTTGAAACAGAAGTTAAGTG+AGG | 0.634933 | 5.3:+78686434 | MS.gene38207:CDS |
| AAAATGCTCCGTCTACCTGG+TGG | 0.635565 | 5.3:+78684890 | MS.gene38207:CDS |
| AAGAACTAGAGAGGATTGTG+AGG | 0.642172 | 5.3:+78686286 | MS.gene38207:CDS |
| TGATCGTGAACTAGCAGACA+AGG | 0.644648 | 5.3:+78686209 | MS.gene38207:CDS |
| ACAAATCACATCCAATCCAA+CGG | 0.645811 | 5.3:-78685300 | None:intergenic |
| GGATGGGGATTCCTTGATGA+GGG | 0.648889 | 5.3:+78685498 | MS.gene38207:CDS |
| GGGATGGGGATTCCTTGATG+AGG | 0.651572 | 5.3:+78685497 | MS.gene38207:CDS |
| AGGCCAAACGAGAGGCTGAG+AGG | 0.658904 | 5.3:+78686352 | MS.gene38207:CDS |
| AAGGTTGATTGCTCCACAGG+AGG | 0.659574 | 5.3:+78686077 | MS.gene38207:CDS |
| ATGGGTCCTCCTGTAAAGAG+TGG | 0.666155 | 5.3:+78685373 | MS.gene38207:CDS |
| TCTCGCTTATCGCAGAGCAG+TGG | 0.671798 | 5.3:+78686507 | MS.gene38207:CDS |
| ACTGATCCACTCTTTACAGG+AGG | 0.681348 | 5.3:-78685379 | None:intergenic |
| CATGGTAGTAAAGACCATCG+TGG | 0.686464 | 5.3:+78685547 | MS.gene38207:CDS |
| GAAACATGCACCAACAACAG+TGG | 0.702295 | 5.3:+78685217 | MS.gene38207:CDS |
| TGGACTTCGAGTTATCCTTG+AGG | 0.704359 | 5.3:+78684934 | MS.gene38207:CDS |
| AAAAGAGGTTCATCCCACGA+TGG | 0.725729 | 5.3:-78685561 | None:intergenic |
| AATACATCAGCATTGAAGAG+GGG | 0.734552 | 5.3:-78686533 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCATTAAAAAAACTAAATT+AGG | + | chr5.3:78685800-78685819 | MS.gene38207:intron | 10.0% |
| !! | AAAATTGAAAAGTAGAAAAA+TGG | + | chr5.3:78685606-78685625 | MS.gene38207:CDS | 15.0% |
| !! | GAATGAGGATAAATAAATAA+AGG | - | chr5.3:78685986-78686005 | None:intergenic | 20.0% |
| !!! | ATTCGTATGTGTTTAAAATT+GGG | - | chr5.3:78685880-78685899 | None:intergenic | 20.0% |
| !!! | TATTCGTATGTGTTTAAAAT+TGG | - | chr5.3:78685881-78685900 | None:intergenic | 20.0% |
| CTCACTTTCTATATCACATT+TGG | + | chr5.3:78685756-78685775 | MS.gene38207:intron | 30.0% | |
| GTATGAGAAAATCAAATTGC+AGG | + | chr5.3:78686479-78686498 | MS.gene38207:CDS | 30.0% | |
| TTTGGTTATGTCTGAACAAA+TGG | + | chr5.3:78685774-78685793 | MS.gene38207:intron | 30.0% | |
| TTTGTTAGTAGGATCTTTCA+TGG | + | chr5.3:78685529-78685548 | MS.gene38207:CDS | 30.0% | |
| ! | TATCTGCATTGACATAATTG+TGG | + | chr5.3:78685712-78685731 | MS.gene38207:intron | 30.0% |
| ! | TTTGAAACAGAAGTTAAGTG+AGG | + | chr5.3:78686434-78686453 | MS.gene38207:CDS | 30.0% |
| !! | GAAAAGCATTTGTTAGAATG+AGG | - | chr5.3:78686001-78686020 | None:intergenic | 30.0% |
| !! | GCAATATTGACTGTTTTTCA+AGG | + | chr5.3:78685649-78685668 | MS.gene38207:CDS | 30.0% |
| ACAAATCACATCCAATCCAA+CGG | - | chr5.3:78685303-78685322 | None:intergenic | 35.0% | |
| AGAAATATCTCAATCAGCGA+TGG | - | chr5.3:78685136-78685155 | None:intergenic | 35.0% | |
| ATCCATGATCTCCTTTACAA+TGG | + | chr5.3:78686561-78686580 | MS.gene38207:CDS | 35.0% | |
| ATCCTTGTTGCCATAAATAC+TGG | + | chr5.3:78685070-78685089 | MS.gene38207:CDS | 35.0% | |
| ATTAGATTGACTGTCTGCTT+TGG | - | chr5.3:78686592-78686611 | None:intergenic | 35.0% | |
| CTTCTGTTTCAAATAGTTGC+TGG | - | chr5.3:78686427-78686446 | None:intergenic | 35.0% | |
| GAAGATGAGAATGTTCAAGA+AGG | + | chr5.3:78686164-78686183 | MS.gene38207:CDS | 35.0% | |
| GCCATAAATACTGGAATTCA+GGG | + | chr5.3:78685079-78685098 | MS.gene38207:CDS | 35.0% | |
| TCCCTGAATTCCAGTATTTA+TGG | - | chr5.3:78685083-78685102 | None:intergenic | 35.0% | |
| TCTTGCGAAGTCTGATAAAT+CGG | + | chr5.3:78686389-78686408 | MS.gene38207:CDS | 35.0% | |
| TGCCATAAATACTGGAATTC+AGG | + | chr5.3:78685078-78685097 | MS.gene38207:CDS | 35.0% | |
| TGGAAAATGTAGAAAGTGGA+AGG | + | chr5.3:78686058-78686077 | MS.gene38207:CDS | 35.0% | |
| TTAGATTGACTGTCTGCTTT+GGG | - | chr5.3:78686591-78686610 | None:intergenic | 35.0% | |
| TTCCAGTATTTATGGCAACA+AGG | - | chr5.3:78685075-78685094 | None:intergenic | 35.0% | |
| TTGAAGTAAATACATGCCGT+TGG | + | chr5.3:78685284-78685303 | MS.gene38207:CDS | 35.0% | |
| ! | AATACATCAGCATTGAAGAG+GGG | - | chr5.3:78686536-78686555 | None:intergenic | 35.0% |
| ! | GAATACATCAGCATTGAAGA+GGG | - | chr5.3:78686537-78686556 | None:intergenic | 35.0% |
| ! | GATGTGATTTGTGTTCTCAT+TGG | + | chr5.3:78685311-78685330 | MS.gene38207:CDS | 35.0% |
| ! | GTAAAGGAGATCATGGATTT+TGG | - | chr5.3:78686559-78686578 | None:intergenic | 35.0% |
| ! | TTCCACTTTCTACATTTTCC+AGG | - | chr5.3:78686059-78686078 | None:intergenic | 35.0% |
| !! | TTTTTAGAGGAGCTGAAAAC+TGG | + | chr5.3:78684914-78684933 | MS.gene38207:CDS | 35.0% |
| AAAATCAAGCTCCCTCATCA+AGG | - | chr5.3:78685512-78685531 | None:intergenic | 40.0% | |
| AAGAACTAGAGAGGATTGTG+AGG | + | chr5.3:78686286-78686305 | MS.gene38207:CDS | 40.0% | |
| AGAAGCAGAAGAACTCAAGA+TGG | + | chr5.3:78686236-78686255 | MS.gene38207:CDS | 40.0% | |
| AGCCTGGAAAATGTAGAAAG+TGG | + | chr5.3:78686054-78686073 | MS.gene38207:CDS | 40.0% | |
| AGTAGGACTTCAGAACTGTT+AGG | + | chr5.3:78685436-78685455 | MS.gene38207:CDS | 40.0% | |
| ATGAGCTCGAATCAATGTCT+TGG | - | chr5.3:78685035-78685054 | None:intergenic | 40.0% | |
| ATGCAACAGAATTGCTGAAG+TGG | + | chr5.3:78686101-78686120 | MS.gene38207:CDS | 40.0% | |
| ATGGTAGTAAAGACCATCGT+GGG | + | chr5.3:78685548-78685567 | MS.gene38207:CDS | 40.0% | |
| CAATTGGAACATATCAGCCT+CGG | - | chr5.3:78686322-78686341 | None:intergenic | 40.0% | |
| CACAGATTGAAGAACTAGAG+AGG | + | chr5.3:78686277-78686296 | MS.gene38207:CDS | 40.0% | |
| CACCTGAAAACCATTTCACT+TGG | - | chr5.3:78685406-78685425 | None:intergenic | 40.0% | |
| CTAAAAATCCACCAGGTAGA+CGG | - | chr5.3:78684901-78684920 | None:intergenic | 40.0% | |
| GGCTGATATGTTCCAATTGA+AGG | + | chr5.3:78686323-78686342 | MS.gene38207:CDS | 40.0% | |
| GTAAATACATGCCGTTGGAT+TGG | + | chr5.3:78685289-78685308 | MS.gene38207:CDS | 40.0% | |
| GTTCCAATTGAAGGCTAATG+AGG | + | chr5.3:78686332-78686351 | MS.gene38207:CDS | 40.0% | |
| TCACAAGAACAATCATCAGC+TGG | - | chr5.3:78685199-78685218 | None:intergenic | 40.0% | |
| ! | GGAATACATCAGCATTGAAG+AGG | - | chr5.3:78686538-78686557 | None:intergenic | 40.0% |
| ! | GGGAGCTTGATTTTGTTAGT+AGG | + | chr5.3:78685518-78685537 | MS.gene38207:CDS | 40.0% |
| ! | TCTACATTTTCCAGGCTCTT+TGG | - | chr5.3:78686051-78686070 | None:intergenic | 40.0% |
| ! | TGCCAAGTGAAATGGTTTTC+AGG | + | chr5.3:78685401-78685420 | MS.gene38207:CDS | 40.0% |
| !! | AGACATTGATTCGAGCTCAT+CGG | + | chr5.3:78685035-78685054 | MS.gene38207:CDS | 40.0% |
| !! | GGTTTTGGCAAGCTATGTTT+CGG | - | chr5.3:78685171-78685190 | None:intergenic | 40.0% |
| !! | TATTCGTCGTGTTTCTAGTG+CGG | + | chr5.3:78684814-78684833 | MS.gene38207:CDS | 40.0% |
| !!! | TCTACCTGGTGGATTTTTAG+AGG | + | chr5.3:78684901-78684920 | MS.gene38207:CDS | 40.0% |
| AAAAGAGGTTCATCCCACGA+TGG | - | chr5.3:78685564-78685583 | None:intergenic | 45.0% | |
| AAAGGCAACAGCAGCATATC+TGG | - | chr5.3:78685968-78685987 | None:intergenic | 45.0% | |
| AACAATCATCAGCTGGAAGC+TGG | - | chr5.3:78685192-78685211 | None:intergenic | 45.0% | |
| ACAGAAGTTAAGTGAGGCTG+AGG | + | chr5.3:78686440-78686459 | MS.gene38207:CDS | 45.0% | |
| ACGGGAGCAACTTATTTGCA+TGG | + | chr5.3:78685354-78685373 | MS.gene38207:CDS | 45.0% | |
| ACTGATCCACTCTTTACAGG+AGG | - | chr5.3:78685382-78685401 | None:intergenic | 45.0% | |
| ATTGCAGAAGCCACTGTTGT+TGG | - | chr5.3:78685230-78685249 | None:intergenic | 45.0% | |
| ATTGTGCACCATCATGGGAT+GGG | + | chr5.3:78685482-78685501 | MS.gene38207:CDS | 45.0% | |
| CAGAAGAACTCAAGATGGAG+AGG | + | chr5.3:78686241-78686260 | MS.gene38207:CDS | 45.0% | |
| CATGGTAGTAAAGACCATCG+TGG | + | chr5.3:78685547-78685566 | MS.gene38207:CDS | 45.0% | |
| CCAACATTGTGCACCATCAT+GGG | + | chr5.3:78685477-78685496 | MS.gene38207:CDS | 45.0% | |
| CGACGAATAACTGACTGATC+AGG | - | chr5.3:78684803-78684822 | None:intergenic | 45.0% | |
| CGGGAGCAACTTATTTGCAT+GGG | + | chr5.3:78685355-78685374 | MS.gene38207:CDS | 45.0% | |
| CTAGGTCAAGCTCTTCATGT+AGG | - | chr5.3:78686023-78686042 | None:intergenic | 45.0% | |
| CTCACACAGATTGTGCCATA+CGG | + | chr5.3:78685335-78685354 | MS.gene38207:CDS | 45.0% | |
| CTTGAGTTCTTCTGCTTCTC+TGG | - | chr5.3:78686235-78686254 | None:intergenic | 45.0% | |
| GAAAAAATGCTCCGTCTACC+TGG | + | chr5.3:78684887-78684906 | MS.gene38207:CDS | 45.0% | |
| GAAACATGCACCAACAACAG+TGG | + | chr5.3:78685217-78685236 | MS.gene38207:CDS | 45.0% | |
| GACTTCAGAACTGTTAGGCT+GGG | + | chr5.3:78685441-78685460 | MS.gene38207:CDS | 45.0% | |
| GATCGTCACACTTCCAAAAG+AGG | - | chr5.3:78685579-78685598 | None:intergenic | 45.0% | |
| GCAAATAAGTTGCTCCCGTA+TGG | - | chr5.3:78685353-78685372 | None:intergenic | 45.0% | |
| GGCCATTGTAAAGGAGATCA+TGG | - | chr5.3:78686566-78686585 | None:intergenic | 45.0% | |
| TCACACAGATTGTGCCATAC+GGG | + | chr5.3:78685336-78685355 | MS.gene38207:CDS | 45.0% | |
| TCAGGTGTCAATCATGCAGT+AGG | + | chr5.3:78685419-78685438 | MS.gene38207:CDS | 45.0% | |
| TCAGTCTTCAGGACATTGCT+CGG | + | chr5.3:78684843-78684862 | MS.gene38207:CDS | 45.0% | |
| TCCAACATTGTGCACCATCA+TGG | + | chr5.3:78685476-78685495 | MS.gene38207:CDS | 45.0% | |
| TGATCGTGAACTAGCAGACA+AGG | + | chr5.3:78686209-78686228 | MS.gene38207:CDS | 45.0% | |
| TGGATCAGTGCCAAGTGAAA+TGG | + | chr5.3:78685393-78685412 | MS.gene38207:CDS | 45.0% | |
| TGTGAGGCTTAAAAATGCCG+AGG | + | chr5.3:78686302-78686321 | MS.gene38207:CDS | 45.0% | |
| ! | AGCTCCTCTAAAAATCCACC+AGG | - | chr5.3:78684908-78684927 | None:intergenic | 45.0% |
| ! | ATCGTGGGATGAACCTCTTT+TGG | + | chr5.3:78685563-78685582 | MS.gene38207:CDS | 45.0% |
| ! | GGACTTCGAGTTATCCTTGA+GGG | + | chr5.3:78684935-78684954 | MS.gene38207:CDS | 45.0% |
| ! | TGGACTTCGAGTTATCCTTG+AGG | + | chr5.3:78684934-78684953 | MS.gene38207:CDS | 45.0% |
| ! | TTCGAGTTATCCTTGAGGGA+GGG | + | chr5.3:78684939-78684958 | MS.gene38207:CDS | 45.0% |
| ! | TTGGAAGTGTGACGATCTCA+AGG | + | chr5.3:78685582-78685601 | MS.gene38207:CDS | 45.0% |
| !! | ATTGACTGTCTGCTTTGGGA+GGG | - | chr5.3:78686587-78686606 | None:intergenic | 45.0% |
| !! | GACATTGATTCGAGCTCATC+GGG | + | chr5.3:78685036-78685055 | MS.gene38207:CDS | 45.0% |
| !! | TGGCCTCATTAGCCTTCAAT+TGG | - | chr5.3:78686338-78686357 | None:intergenic | 45.0% |
| AAAATGCTCCGTCTACCTGG+TGG | + | chr5.3:78684890-78684909 | MS.gene38207:CDS | 50.0% | |
| AAGGAATCCCCATCCCATGA+TGG | - | chr5.3:78685493-78685512 | None:intergenic | 50.0% | |
| AATTGCTGAAGTGGTGCAGG+AGG | + | chr5.3:78686110-78686129 | MS.gene38207:CDS | 50.0% | |
| ACTTCGCAAGAGCAATCCTC+TGG | - | chr5.3:78686380-78686399 | None:intergenic | 50.0% | |
| ATGGGTCCTCCTGTAAAGAG+TGG | + | chr5.3:78685373-78685392 | MS.gene38207:CDS | 50.0% | |
| ATTCTGTTGCATGCCTCCTG+TGG | - | chr5.3:78686093-78686112 | None:intergenic | 50.0% | |
| CAGAATTGCTGAAGTGGTGC+AGG | + | chr5.3:78686107-78686126 | MS.gene38207:CDS | 50.0% | |
| CAGTCTTCAGGACATTGCTC+GGG | + | chr5.3:78684844-78684863 | MS.gene38207:CDS | 50.0% | |
| CATTGTGCACCATCATGGGA+TGG | + | chr5.3:78685481-78685500 | MS.gene38207:CDS | 50.0% | |
| CTAATGACTGCCACCCTTTC+CGG | + | chr5.3:78686607-78686626 | MS.gene38207:CDS | 50.0% | |
| GCAGGAGGCTATACGAAAGA+TGG | + | chr5.3:78686125-78686144 | MS.gene38207:CDS | 50.0% | |
| GGAAGCTAAAGCAGCATGCA+GGG | + | chr5.3:78685627-78685646 | MS.gene38207:CDS | 50.0% | |
| GGACTTCAGAACTGTTAGGC+TGG | + | chr5.3:78685440-78685459 | MS.gene38207:CDS | 50.0% | |
| GGATGGGGATTCCTTGATGA+GGG | + | chr5.3:78685498-78685517 | MS.gene38207:CDS | 50.0% | |
| GGCTGCGACAGACTAATGTT+AGG | - | chr5.3:78685115-78685134 | None:intergenic | 50.0% | |
| GTTATCCTTGAGGGAGGGAA+TGG | + | chr5.3:78684944-78684963 | MS.gene38207:CDS | 50.0% | |
| TGCGGACAGAATCAGTCTTC+AGG | + | chr5.3:78684832-78684851 | MS.gene38207:CDS | 50.0% | |
| TGGAAGCTAAAGCAGCATGC+AGG | + | chr5.3:78685626-78685645 | MS.gene38207:CDS | 50.0% | |
| TTGTGCACCATCATGGGATG+GGG | + | chr5.3:78685483-78685502 | MS.gene38207:CDS | 50.0% | |
| ! | AAGGTTGATTGCTCCACAGG+AGG | + | chr5.3:78686077-78686096 | MS.gene38207:CDS | 50.0% |
| ! | CCCATGATGGTGCACAATGT+TGG | - | chr5.3:78685480-78685499 | None:intergenic | 50.0% |
| ! | CTTCGAGTTATCCTTGAGGG+AGG | + | chr5.3:78684938-78684957 | MS.gene38207:CDS | 50.0% |
| ! | CTTTGGGAGGGCCATTGTAA+AGG | - | chr5.3:78686575-78686594 | None:intergenic | 50.0% |
| ! | GATTGACTGTCTGCTTTGGG+AGG | - | chr5.3:78686588-78686607 | None:intergenic | 50.0% |
| ! | TGGAAGGTTGATTGCTCCAC+AGG | + | chr5.3:78686074-78686093 | MS.gene38207:CDS | 50.0% |
| !! | CATCAGCTGGAAGCTGGTTT+TGG | - | chr5.3:78685186-78685205 | None:intergenic | 50.0% |
| !! | GGCACTGATCCACTCTTTAC+AGG | - | chr5.3:78685385-78685404 | None:intergenic | 50.0% |
| CCTAGACTCTCCAAAGAGCC+TGG | + | chr5.3:78686038-78686057 | MS.gene38207:CDS | 55.0% | |
| GAAGGCTCGCTTAGCTGTTG+AGG | + | chr5.3:78686182-78686201 | MS.gene38207:CDS | 55.0% | |
| GGACATTGCTCGGGAAAGAG+TGG | + | chr5.3:78684853-78684872 | MS.gene38207:CDS | 55.0% | |
| GGCTAATGAGGCCAAACGAG+AGG | + | chr5.3:78686344-78686363 | MS.gene38207:CDS | 55.0% | |
| GGGATGGGGATTCCTTGATG+AGG | + | chr5.3:78685497-78685516 | MS.gene38207:CDS | 55.0% | |
| TCTCGCTTATCGCAGAGCAG+TGG | + | chr5.3:78686507-78686526 | MS.gene38207:CDS | 55.0% | |
| ! | CCAGGCTCTTTGGAGAGTCT+AGG | - | chr5.3:78686041-78686060 | None:intergenic | 55.0% |
| ACGGGTTTGTCCGGAAAGGG+TGG | - | chr5.3:78686620-78686639 | None:intergenic | 60.0% | |
| AGGCCAAACGAGAGGCTGAG+AGG | + | chr5.3:78686352-78686371 | MS.gene38207:CDS | 60.0% | |
| GAGCCTCTCAGCCTCTCGTT+TGG | - | chr5.3:78686358-78686377 | None:intergenic | 60.0% | |
| GCATGCCATTCCCTCCCTCA+AGG | - | chr5.3:78684952-78684971 | None:intergenic | 60.0% | |
| GAGAGGCTGAGAGGCTCCAG+AGG | + | chr5.3:78686361-78686380 | MS.gene38207:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 78684770 | 78686641 | 78684770 | ID=MS.gene38207 |
| chr5.3 | mRNA | 78684770 | 78686641 | 78684770 | ID=MS.gene38207.t1;Parent=MS.gene38207 |
| chr5.3 | exon | 78684770 | 78685670 | 78684770 | ID=MS.gene38207.t1.exon1;Parent=MS.gene38207.t1 |
| chr5.3 | CDS | 78684770 | 78685670 | 78684770 | ID=cds.MS.gene38207.t1;Parent=MS.gene38207.t1 |
| chr5.3 | exon | 78686031 | 78686641 | 78686031 | ID=MS.gene38207.t1.exon2;Parent=MS.gene38207.t1 |
| chr5.3 | CDS | 78686031 | 78686641 | 78686031 | ID=cds.MS.gene38207.t1;Parent=MS.gene38207.t1 |
| Gene Sequence |
| Protein sequence |
