Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061884.t1 | XP_003625487.2 | 100 | 95 | 0 | 0 | 1 | 95 | 555 | 649 | 1.60E-44 | 188.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061884.t1 | Q9LHA8 | 79.4 | 97 | 17 | 3 | 1 | 95 | 555 | 650 | 7.0e-33 | 141.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061884.t1 | Q2HT97 | 100.0 | 95 | 0 | 0 | 1 | 95 | 555 | 649 | 1.1e-44 | 188.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene061884 | 0.800525 | 1.38E-48 | -1.69E-46 |
| MS.gene049887 | MS.gene061884 | 0.817765 | 2.78E-52 | -1.69E-46 |
| MS.gene050277 | MS.gene061884 | 0.825297 | 5.06E-54 | -1.69E-46 |
| MS.gene050815 | MS.gene061884 | 0.806149 | 9.46E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene061884 | 0.851553 | 8.24E-61 | -1.69E-46 |
| MS.gene051063 | MS.gene061884 | 0.82336 | 1.44E-53 | -1.69E-46 |
| MS.gene051245 | MS.gene061884 | 0.813055 | 3.11E-51 | -1.69E-46 |
| MS.gene051328 | MS.gene061884 | 0.828699 | 7.78E-55 | -1.69E-46 |
| MS.gene051746 | MS.gene061884 | 0.85024 | 1.93E-60 | -1.69E-46 |
| MS.gene051748 | MS.gene061884 | 0.83299 | 6.90E-56 | -1.69E-46 |
| MS.gene051850 | MS.gene061884 | 0.84587 | 3.10E-59 | -1.69E-46 |
| MS.gene051903 | MS.gene061884 | 0.8292 | 5.88E-55 | -1.69E-46 |
| MS.gene052434 | MS.gene061884 | 0.802218 | 6.23E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene061884 | 0.833289 | 5.81E-56 | -1.69E-46 |
| MS.gene053109 | MS.gene061884 | 0.843356 | 1.47E-58 | -1.69E-46 |
| MS.gene053381 | MS.gene061884 | 0.81932 | 1.24E-52 | -1.69E-46 |
| MS.gene054250 | MS.gene061884 | 0.805488 | 1.30E-49 | -1.69E-46 |
| MS.gene055162 | MS.gene061884 | 0.812207 | 4.76E-51 | -1.69E-46 |
| MS.gene055174 | MS.gene061884 | 0.834501 | 2.89E-56 | -1.69E-46 |
| MS.gene055468 | MS.gene061884 | 0.806373 | 8.49E-50 | -1.69E-46 |
| MS.gene056069 | MS.gene061884 | -0.800934 | 1.14E-48 | -1.69E-46 |
| MS.gene056432 | MS.gene061884 | 0.859377 | 4.31E-63 | -1.69E-46 |
| MS.gene056580 | MS.gene061884 | 0.828009 | 1.14E-54 | -1.69E-46 |
| MS.gene056631 | MS.gene061884 | 0.814894 | 1.22E-51 | -1.69E-46 |
| MS.gene056752 | MS.gene061884 | 0.803033 | 4.23E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene061884 | 0.857996 | 1.11E-62 | -1.69E-46 |
| MS.gene057094 | MS.gene061884 | 0.829578 | 4.76E-55 | -1.69E-46 |
| MS.gene057140 | MS.gene061884 | 0.825972 | 3.50E-54 | -1.69E-46 |
| MS.gene057234 | MS.gene061884 | 0.867047 | 1.83E-65 | -1.69E-46 |
| MS.gene058055 | MS.gene061884 | 0.833399 | 5.45E-56 | -1.69E-46 |
| MS.gene058084 | MS.gene061884 | 0.817881 | 2.62E-52 | -1.69E-46 |
| MS.gene058182 | MS.gene061884 | 0.816445 | 5.51E-52 | -1.69E-46 |
| MS.gene058646 | MS.gene061884 | 0.812808 | 3.52E-51 | -1.69E-46 |
| MS.gene058654 | MS.gene061884 | 0.832871 | 7.38E-56 | -1.69E-46 |
| MS.gene058906 | MS.gene061884 | 0.855545 | 5.87E-62 | -1.69E-46 |
| MS.gene059898 | MS.gene061884 | 0.85039 | 1.75E-60 | -1.69E-46 |
| MS.gene06001 | MS.gene061884 | 0.853553 | 2.21E-61 | -1.69E-46 |
| MS.gene06011 | MS.gene061884 | 0.825176 | 5.41E-54 | -1.69E-46 |
| MS.gene060463 | MS.gene061884 | 0.810616 | 1.05E-50 | -1.69E-46 |
| MS.gene060465 | MS.gene061884 | 0.811191 | 7.92E-51 | -1.69E-46 |
| MS.gene060869 | MS.gene061884 | 0.834689 | 2.59E-56 | -1.69E-46 |
| MS.gene060870 | MS.gene061884 | 0.816035 | 6.81E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061884.t1 | MTR_7g099680 | 100.000 | 95 | 0 | 0 | 1 | 95 | 555 | 649 | 1.74e-60 | 194 |
| MS.gene061884.t1 | MTR_2g040355 | 84.783 | 92 | 13 | 1 | 2 | 93 | 212 | 302 | 1.42e-45 | 148 |
| MS.gene061884.t1 | MTR_7g024580 | 76.000 | 100 | 14 | 3 | 1 | 95 | 555 | 649 | 8.55e-41 | 141 |
| MS.gene061884.t1 | MTR_7g024390 | 77.895 | 95 | 20 | 1 | 1 | 95 | 555 | 648 | 3.82e-37 | 131 |
| MS.gene061884.t1 | MTR_4g063720 | 73.684 | 95 | 25 | 0 | 1 | 95 | 484 | 578 | 1.88e-36 | 129 |
| MS.gene061884.t1 | MTR_4g063720 | 73.684 | 95 | 25 | 0 | 1 | 95 | 555 | 649 | 2.30e-36 | 129 |
| MS.gene061884.t1 | MTR_4g063710 | 73.196 | 97 | 22 | 2 | 1 | 95 | 555 | 649 | 5.18e-36 | 128 |
| MS.gene061884.t1 | MTR_4g130540 | 65.000 | 100 | 30 | 1 | 1 | 95 | 554 | 653 | 2.53e-33 | 120 |
| MS.gene061884.t1 | MTR_2g040220 | 58.947 | 95 | 34 | 2 | 4 | 95 | 1466 | 1558 | 8.44e-26 | 99.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061884.t1 | AT3G09440 | 74.737 | 95 | 24 | 0 | 1 | 95 | 555 | 649 | 7.74e-39 | 136 |
| MS.gene061884.t1 | AT3G09440 | 74.737 | 95 | 24 | 0 | 1 | 95 | 555 | 649 | 7.74e-39 | 136 |
| MS.gene061884.t1 | AT3G09440 | 74.737 | 95 | 24 | 0 | 1 | 95 | 555 | 649 | 7.74e-39 | 136 |
| MS.gene061884.t1 | AT3G09440 | 74.737 | 95 | 24 | 0 | 1 | 95 | 555 | 649 | 7.74e-39 | 136 |
| MS.gene061884.t1 | AT5G02500 | 73.196 | 97 | 24 | 1 | 1 | 95 | 425 | 521 | 6.80e-37 | 130 |
| MS.gene061884.t1 | AT3G12580 | 77.320 | 97 | 19 | 2 | 1 | 95 | 555 | 650 | 1.17e-36 | 130 |
| MS.gene061884.t1 | AT5G02500 | 73.196 | 97 | 24 | 1 | 1 | 95 | 555 | 651 | 1.39e-36 | 130 |
| MS.gene061884.t1 | AT5G02490 | 71.717 | 99 | 24 | 1 | 1 | 95 | 555 | 653 | 2.45e-35 | 126 |
| MS.gene061884.t1 | AT1G16030 | 63.158 | 95 | 33 | 1 | 1 | 95 | 554 | 646 | 7.97e-34 | 122 |
| MS.gene061884.t1 | AT1G56410 | 66.667 | 63 | 19 | 1 | 3 | 65 | 557 | 617 | 3.19e-19 | 80.9 |
| MS.gene061884.t1 | AT5G42020 | 46.753 | 77 | 35 | 2 | 1 | 76 | 580 | 651 | 7.02e-16 | 71.6 |
| MS.gene061884.t1 | AT5G42020 | 46.753 | 77 | 35 | 2 | 1 | 76 | 585 | 656 | 7.23e-16 | 71.6 |
| MS.gene061884.t1 | AT5G42020 | 46.753 | 77 | 35 | 2 | 1 | 76 | 525 | 596 | 8.15e-16 | 71.2 |
| MS.gene061884.t1 | AT5G28540 | 49.231 | 65 | 32 | 1 | 1 | 64 | 580 | 644 | 2.94e-15 | 69.7 |
| MS.gene061884.t1 | AT1G09080 | 40.000 | 65 | 38 | 1 | 1 | 64 | 584 | 648 | 2.16e-11 | 58.9 |
| MS.gene061884.t1 | AT1G09080 | 40.000 | 65 | 38 | 1 | 1 | 64 | 594 | 658 | 2.29e-11 | 58.5 |
Find 36 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGTCAACCTCCTCAATCTT+AGG | 0.192326 | 7.4:+10615600 | None:intergenic |
| CCTTCCCATGCAGGCGGTGC+TGG | 0.268674 | 7.4:-10615624 | MS.gene061884:CDS |
| TGGTACATCTTGGCAATGAT+AGG | 0.336601 | 7.4:+10615696 | None:intergenic |
| CAACATGAGGAACACCATTA+AGG | 0.368890 | 7.4:-10615868 | None:intergenic |
| CCTGACATGGGTGCTGCTCC+TGG | 0.370835 | 7.4:-10615660 | MS.gene061884:CDS |
| ATTAAGGATGAAAAGATTGC+AGG | 0.418231 | 7.4:-10615852 | MS.gene061884:CDS |
| AGTCAACCTCCTCAATCTTA+GGG | 0.447999 | 7.4:+10615601 | None:intergenic |
| CTCAATCTTCTTCTTGTCAT+CGG | 0.449484 | 7.4:+10615818 | None:intergenic |
| TGCACCACCTTGGTACATCT+TGG | 0.469054 | 7.4:+10615686 | None:intergenic |
| TAGGGCCAGCACCGCCTGCA+TGG | 0.469475 | 7.4:+10615619 | None:intergenic |
| TGCAGAGGCAGATGAGTTTG+AGG | 0.473213 | 7.4:-10615751 | MS.gene061884:CDS |
| AGGGCCAGCACCGCCTGCAT+GGG | 0.495476 | 7.4:+10615620 | None:intergenic |
| ATCTGCCTCTGCAAGCTGGT+TGG | 0.501322 | 7.4:+10615761 | None:intergenic |
| CCAGCACCGCCTGCATGGGA+AGG | 0.501889 | 7.4:+10615624 | None:intergenic |
| AAGATGTACCAAGGTGGTGC+AGG | 0.511591 | 7.4:-10615684 | MS.gene061884:CDS |
| CGGTGCTGGCCCTAAGATTG+AGG | 0.526294 | 7.4:-10615610 | MS.gene061884:CDS |
| CCACTGGATAGCAGCCTCAA+TGG | 0.526619 | 7.4:+10615791 | None:intergenic |
| ACTCATCTGCCTCTGCAAGC+TGG | 0.530015 | 7.4:+10615757 | None:intergenic |
| GGTACATCTTGGCAATGATA+GGG | 0.535730 | 7.4:+10615697 | None:intergenic |
| GCTGGTTGGCATCTAGCCAC+TGG | 0.543227 | 7.4:+10615775 | None:intergenic |
| GATGATGCTCCTTCCCATGC+AGG | 0.543328 | 7.4:-10615633 | MS.gene061884:CDS |
| CCAGGAGCAGCACCCATGTC+AGG | 0.553016 | 7.4:+10615660 | None:intergenic |
| GACAAGATGAAGGAACTTGA+AGG | 0.560443 | 7.4:-10615729 | MS.gene061884:CDS |
| TGAGTTTGAGGACAAGATGA+AGG | 0.572507 | 7.4:-10615739 | MS.gene061884:CDS |
| AGGTGGTGCAGGTCCTGACA+TGG | 0.578395 | 7.4:-10615673 | MS.gene061884:CDS |
| CGATGACAAGAAGAAGATTG+AGG | 0.584147 | 7.4:-10615817 | MS.gene061884:CDS |
| GGTGGTGCAGGTCCTGACAT+GGG | 0.603706 | 7.4:-10615672 | MS.gene061884:CDS |
| TGTCAGGACCTGCACCACCT+TGG | 0.615607 | 7.4:+10615676 | None:intergenic |
| GAAGGAGCATCATCATCACC+AGG | 0.624959 | 7.4:+10615642 | None:intergenic |
| GAAGATTGAGGACACCATTG+AGG | 0.632637 | 7.4:-10615805 | MS.gene061884:CDS |
| ATCATTGCCAAGATGTACCA+AGG | 0.641899 | 7.4:-10615693 | MS.gene061884:CDS |
| AGATGCCAACCAGCTTGCAG+AGG | 0.642658 | 7.4:-10615766 | MS.gene061884:CDS |
| TGCTGGCCCTAAGATTGAGG+AGG | 0.651604 | 7.4:-10615607 | MS.gene061884:CDS |
| CCATTGAGGCTGCTATCCAG+TGG | 0.659592 | 7.4:-10615791 | MS.gene061884:CDS |
| GATGCTCCTTCCCATGCAGG+CGG | 0.675350 | 7.4:-10615630 | MS.gene061884:CDS |
| ATTGCCAAGATGTACCAAGG+TGG | 0.774020 | 7.4:-10615690 | MS.gene061884:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTAAGGATGAAAAGATTGC+AGG | - | chr7.4:10615611-10615630 | MS.gene061884:CDS | 30.0% | |
| ! | TGCAATCTTTTCATCCTTAA+TGG | + | chr7.4:10615612-10615631 | None:intergenic | 30.0% |
| CTCAATCTTCTTCTTGTCAT+CGG | + | chr7.4:10615648-10615667 | None:intergenic | 35.0% | |
| AGTCAACCTCCTCAATCTTA+GGG | + | chr7.4:10615865-10615884 | None:intergenic | 40.0% | |
| ATCATTGCCAAGATGTACCA+AGG | - | chr7.4:10615770-10615789 | MS.gene061884:CDS | 40.0% | |
| CGATGACAAGAAGAAGATTG+AGG | - | chr7.4:10615646-10615665 | MS.gene061884:CDS | 40.0% | |
| GACAAGATGAAGGAACTTGA+AGG | - | chr7.4:10615734-10615753 | MS.gene061884:CDS | 40.0% | |
| GGTACATCTTGGCAATGATA+GGG | + | chr7.4:10615769-10615788 | None:intergenic | 40.0% | |
| TAGTCAACCTCCTCAATCTT+AGG | + | chr7.4:10615866-10615885 | None:intergenic | 40.0% | |
| TGAGTTTGAGGACAAGATGA+AGG | - | chr7.4:10615724-10615743 | MS.gene061884:CDS | 40.0% | |
| TGGTACATCTTGGCAATGAT+AGG | + | chr7.4:10615770-10615789 | None:intergenic | 40.0% | |
| ATTGCCAAGATGTACCAAGG+TGG | - | chr7.4:10615773-10615792 | MS.gene061884:CDS | 45.0% | |
| GAAGATTGAGGACACCATTG+AGG | - | chr7.4:10615658-10615677 | MS.gene061884:CDS | 45.0% | |
| AAGATGTACCAAGGTGGTGC+AGG | - | chr7.4:10615779-10615798 | MS.gene061884:CDS | 50.0% | |
| TGCACCACCTTGGTACATCT+TGG | + | chr7.4:10615780-10615799 | None:intergenic | 50.0% | |
| TGCAGAGGCAGATGAGTTTG+AGG | - | chr7.4:10615712-10615731 | MS.gene061884:CDS | 50.0% | |
| !! | GAAGGAGCATCATCATCACC+AGG | + | chr7.4:10615824-10615843 | None:intergenic | 50.0% |
| ACTCATCTGCCTCTGCAAGC+TGG | + | chr7.4:10615709-10615728 | None:intergenic | 55.0% | |
| AGATGCCAACCAGCTTGCAG+AGG | - | chr7.4:10615697-10615716 | MS.gene061884:CDS | 55.0% | |
| ATCTGCCTCTGCAAGCTGGT+TGG | + | chr7.4:10615705-10615724 | None:intergenic | 55.0% | |
| CCACTGGATAGCAGCCTCAA+TGG | + | chr7.4:10615675-10615694 | None:intergenic | 55.0% | |
| CCATTGAGGCTGCTATCCAG+TGG | - | chr7.4:10615672-10615691 | MS.gene061884:CDS | 55.0% | |
| GATGATGCTCCTTCCCATGC+AGG | - | chr7.4:10615830-10615849 | MS.gene061884:CDS | 55.0% | |
| TGCTGGCCCTAAGATTGAGG+AGG | - | chr7.4:10615856-10615875 | MS.gene061884:CDS | 55.0% | |
| AGGTGGTGCAGGTCCTGACA+TGG | - | chr7.4:10615790-10615809 | MS.gene061884:CDS | 60.0% | |
| GATGCTCCTTCCCATGCAGG+CGG | - | chr7.4:10615833-10615852 | MS.gene061884:CDS | 60.0% | |
| GCTGGTTGGCATCTAGCCAC+TGG | + | chr7.4:10615691-10615710 | None:intergenic | 60.0% | |
| GGTGGTGCAGGTCCTGACAT+GGG | - | chr7.4:10615791-10615810 | MS.gene061884:CDS | 60.0% | |
| TGTCAGGACCTGCACCACCT+TGG | + | chr7.4:10615790-10615809 | None:intergenic | 60.0% | |
| !! | CGGTGCTGGCCCTAAGATTG+AGG | - | chr7.4:10615853-10615872 | MS.gene061884:CDS | 60.0% |
| CCAGGAGCAGCACCCATGTC+AGG | + | chr7.4:10615806-10615825 | None:intergenic | 65.0% | |
| !! | CCTGACATGGGTGCTGCTCC+TGG | - | chr7.4:10615803-10615822 | MS.gene061884:CDS | 65.0% |
| AGGGCCAGCACCGCCTGCAT+GGG | + | chr7.4:10615846-10615865 | None:intergenic | 70.0% | |
| CCAGCACCGCCTGCATGGGA+AGG | + | chr7.4:10615842-10615861 | None:intergenic | 70.0% | |
| CCTTCCCATGCAGGCGGTGC+TGG | - | chr7.4:10615839-10615858 | MS.gene061884:CDS | 70.0% | |
| TAGGGCCAGCACCGCCTGCA+TGG | + | chr7.4:10615847-10615866 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10615599 | 10615886 | 10615599 | ID=MS.gene061884 |
| chr7.4 | mRNA | 10615599 | 10615886 | 10615599 | ID=MS.gene061884.t1;Parent=MS.gene061884 |
| chr7.4 | exon | 10615599 | 10615886 | 10615599 | ID=MS.gene061884.t1.exon1;Parent=MS.gene061884.t1 |
| chr7.4 | CDS | 10615599 | 10615886 | 10615599 | ID=cds.MS.gene061884.t1;Parent=MS.gene061884.t1 |
| Gene Sequence |
| Protein sequence |
