Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06421.t1 | XP_003602589.1 | 98.9 | 177 | 2 | 0 | 1 | 177 | 1 | 177 | 1.30E-92 | 349 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06421.t1 | O49603 | 50.3 | 179 | 86 | 1 | 1 | 176 | 1 | 179 | 3.3e-44 | 179.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06421.t1 | G7JBI0 | 98.9 | 177 | 2 | 0 | 1 | 177 | 1 | 177 | 9.5e-93 | 349.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049298 | MS.gene06421 | 0.946963 | 1.69E-105 | -1.69E-46 |
| MS.gene050326 | MS.gene06421 | 0.803547 | 3.31E-49 | -1.69E-46 |
| MS.gene050338 | MS.gene06421 | 0.83123 | 1.88E-55 | -1.69E-46 |
| MS.gene050707 | MS.gene06421 | 0.838731 | 2.42E-57 | -1.69E-46 |
| MS.gene050710 | MS.gene06421 | 0.808436 | 3.10E-50 | -1.69E-46 |
| MS.gene050875 | MS.gene06421 | 0.807715 | 4.41E-50 | -1.69E-46 |
| MS.gene051914 | MS.gene06421 | 0.814505 | 1.49E-51 | -1.69E-46 |
| MS.gene052169 | MS.gene06421 | 0.82253 | 2.25E-53 | -1.69E-46 |
| MS.gene052209 | MS.gene06421 | 0.825933 | 3.58E-54 | -1.69E-46 |
| MS.gene052308 | MS.gene06421 | 0.843835 | 1.10E-58 | -1.69E-46 |
| MS.gene053108 | MS.gene06421 | 0.8052 | 1.50E-49 | -1.69E-46 |
| MS.gene054551 | MS.gene06421 | 0.822646 | 2.12E-53 | -1.69E-46 |
| MS.gene054593 | MS.gene06421 | 0.812889 | 3.38E-51 | -1.69E-46 |
| MS.gene054633 | MS.gene06421 | 0.80483 | 1.79E-49 | -1.69E-46 |
| MS.gene054662 | MS.gene06421 | 0.811509 | 6.76E-51 | -1.69E-46 |
| MS.gene054850 | MS.gene06421 | 0.815815 | 7.62E-52 | -1.69E-46 |
| MS.gene054941 | MS.gene06421 | 0.999072 | 8.64E-289 | -1.69E-46 |
| MS.gene055308 | MS.gene06421 | 0.812292 | 4.56E-51 | -1.69E-46 |
| MS.gene055578 | MS.gene06421 | 0.811773 | 5.92E-51 | -1.69E-46 |
| MS.gene056041 | MS.gene06421 | 0.800808 | 1.21E-48 | -1.69E-46 |
| MS.gene056387 | MS.gene06421 | -0.854239 | 1.41E-61 | -1.69E-46 |
| MS.gene056528 | MS.gene06421 | 0.807679 | 4.49E-50 | -1.69E-46 |
| MS.gene05652 | MS.gene06421 | 0.873507 | 1.39E-67 | -1.69E-46 |
| MS.gene056618 | MS.gene06421 | 0.848205 | 7.11E-60 | -1.69E-46 |
| MS.gene056697 | MS.gene06421 | 0.812499 | 4.11E-51 | -1.69E-46 |
| MS.gene057101 | MS.gene06421 | 0.8182 | 2.22E-52 | -1.69E-46 |
| MS.gene057356 | MS.gene06421 | 0.811568 | 6.56E-51 | -1.69E-46 |
| MS.gene057367 | MS.gene06421 | 0.82123 | 4.51E-53 | -1.69E-46 |
| MS.gene057669 | MS.gene06421 | 0.866532 | 2.66E-65 | -1.69E-46 |
| MS.gene057717 | MS.gene06421 | 0.851427 | 8.94E-61 | -1.69E-46 |
| MS.gene058128 | MS.gene06421 | 0.866423 | 2.88E-65 | -1.69E-46 |
| MS.gene058853 | MS.gene06421 | 0.869868 | 2.24E-66 | -1.69E-46 |
| MS.gene05926 | MS.gene06421 | 0.876737 | 1.10E-68 | -1.69E-46 |
| MS.gene059616 | MS.gene06421 | 0.865596 | 5.26E-65 | -1.69E-46 |
| MS.gene059905 | MS.gene06421 | 0.810924 | 9.05E-51 | -1.69E-46 |
| MS.gene060985 | MS.gene06421 | 0.823167 | 1.60E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06421.t1 | MTR_3g095980 | 98.870 | 177 | 2 | 0 | 1 | 177 | 1 | 177 | 6.06e-129 | 359 |
| MS.gene06421.t1 | MTR_4g122740 | 29.310 | 174 | 114 | 4 | 5 | 177 | 13 | 178 | 7.52e-17 | 74.3 |
| MS.gene06421.t1 | MTR_2g033630 | 32.335 | 167 | 106 | 5 | 12 | 176 | 15 | 176 | 5.16e-16 | 72.0 |
| MS.gene06421.t1 | MTR_4g122890 | 30.726 | 179 | 105 | 6 | 7 | 176 | 7 | 175 | 7.75e-15 | 68.9 |
| MS.gene06421.t1 | MTR_8g009300 | 26.012 | 173 | 116 | 6 | 13 | 177 | 13 | 181 | 4.57e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06421.t1 | AT5G64620 | 50.000 | 180 | 87 | 1 | 1 | 177 | 1 | 180 | 2.61e-63 | 193 |
| MS.gene06421.t1 | AT3G17220 | 27.879 | 165 | 114 | 3 | 12 | 176 | 14 | 173 | 2.08e-13 | 65.1 |
| MS.gene06421.t1 | AT4G15750 | 29.197 | 137 | 95 | 1 | 9 | 145 | 8 | 142 | 4.80e-13 | 64.3 |
| MS.gene06421.t1 | AT5G46930 | 28.313 | 166 | 104 | 6 | 5 | 161 | 1 | 160 | 4.11e-11 | 59.3 |
Find 38 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACATAGATTGTAGTATT+TGG | 0.248640 | 3.2:+80171811 | None:intergenic |
| ATTCAAAAGGGTTCCTGATT+TGG | 0.274182 | 3.2:-80171492 | MS.gene06421:CDS |
| GAGCTTGCCACTAGAGAAAA+TGG | 0.275669 | 3.2:-80171458 | MS.gene06421:CDS |
| GCACACTATGCATTTGTAAA+TGG | 0.303461 | 3.2:-80171869 | MS.gene06421:CDS |
| CCAAGCTTCAGTACAAGATT+TGG | 0.336276 | 3.2:-80171588 | MS.gene06421:CDS |
| AGGCCCTTTGTGTCTGCGTT+TGG | 0.336742 | 3.2:+80171761 | None:intergenic |
| GGTTGGAGTAAGAGATTTAG+AGG | 0.338526 | 3.2:+80171679 | None:intergenic |
| CAACAACACTACCTTGAAAA+AGG | 0.368143 | 3.2:-80171657 | MS.gene06421:CDS |
| ACAAGCATTTGGGTAGTCTT+TGG | 0.377171 | 3.2:+80171523 | None:intergenic |
| ACTTATCTGCACACTCTTTG+AGG | 0.396879 | 3.2:+80171633 | None:intergenic |
| AATGCATTGTGACAAGCATT+TGG | 0.398656 | 3.2:+80171512 | None:intergenic |
| CTTGTCACAATGCATTCAAA+AGG | 0.421269 | 3.2:-80171505 | MS.gene06421:CDS |
| TCAGGAGGGTAAACCAAATC+AGG | 0.429293 | 3.2:+80171479 | None:intergenic |
| GCCTAGCTGTGATCATGGTT+GGG | 0.442882 | 3.2:-80171742 | MS.gene06421:CDS |
| TGCGTTTGGACTGCTAGGAT+TGG | 0.446964 | 3.2:+80171775 | None:intergenic |
| TTCAAGGTAGTGTTGTTGGT+TGG | 0.453115 | 3.2:+80171662 | None:intergenic |
| CCAAATCTTGTACTGAAGCT+TGG | 0.454754 | 3.2:+80171588 | None:intergenic |
| GTGTCTGCGTTTGGACTGCT+AGG | 0.461087 | 3.2:+80171770 | None:intergenic |
| GGCCTAGCTGTGATCATGGT+TGG | 0.485514 | 3.2:-80171743 | MS.gene06421:CDS |
| CATATATGTGATGTAGCTAT+GGG | 0.497869 | 3.2:-80171428 | MS.gene06421:CDS |
| ATGCATTGTGACAAGCATTT+GGG | 0.500606 | 3.2:+80171513 | None:intergenic |
| ACATATATGTGATGTAGCTA+TGG | 0.505961 | 3.2:-80171429 | MS.gene06421:CDS |
| GGATGAGAAATAGTGAGAAG+TGG | 0.509606 | 3.2:+80171900 | None:intergenic |
| TTACAAATGCATAGTGTGCT+AGG | 0.521367 | 3.2:+80171873 | None:intergenic |
| AAAGGGCCTAGCTGTGATCA+TGG | 0.521391 | 3.2:-80171747 | MS.gene06421:CDS |
| GCTGTGATCATGGTTGGGAT+TGG | 0.523662 | 3.2:-80171737 | MS.gene06421:CDS |
| TCTCTAGTGGCAAGCTCAGG+AGG | 0.523727 | 3.2:+80171464 | None:intergenic |
| AATCTTGTACTGAAGCTTGG+AGG | 0.528434 | 3.2:+80171591 | None:intergenic |
| ATGCATAGTGTGCTAGGAAG+AGG | 0.536460 | 3.2:+80171879 | None:intergenic |
| AGTCCAAACGCAGACACAAA+GGG | 0.542036 | 3.2:-80171764 | MS.gene06421:CDS |
| ATCTTGTACTGAAGCTTGGA+GGG | 0.545648 | 3.2:+80171592 | None:intergenic |
| AGAGGACAAATATGAAGAAG+TGG | 0.553621 | 3.2:+80171697 | None:intergenic |
| TTGTCACAATGCATTCAAAA+GGG | 0.564017 | 3.2:-80171504 | MS.gene06421:CDS |
| CTCTAGTGGCAAGCTCAGGA+GGG | 0.593107 | 3.2:+80171465 | None:intergenic |
| CAGTCCAAACGCAGACACAA+AGG | 0.620929 | 3.2:-80171765 | MS.gene06421:CDS |
| TCCCAACCATGATCACAGCT+AGG | 0.662886 | 3.2:+80171741 | None:intergenic |
| GCAGATAAGTACAACTATGC+TGG | 0.666996 | 3.2:-80171620 | MS.gene06421:CDS |
| ATATATGTGATGTAGCTATG+GGG | 0.736245 | 3.2:-80171427 | MS.gene06421:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAACATAGATTGTAGTATT+TGG | + | chr3.2:80171510-80171529 | None:intergenic | 25.0% |
| ACATATATGTGATGTAGCTA+TGG | - | chr3.2:80171889-80171908 | MS.gene06421:CDS | 30.0% | |
| ATATATGTGATGTAGCTATG+GGG | - | chr3.2:80171891-80171910 | MS.gene06421:CDS | 30.0% | |
| CATATATGTGATGTAGCTAT+GGG | - | chr3.2:80171890-80171909 | MS.gene06421:CDS | 30.0% | |
| TTGTCACAATGCATTCAAAA+GGG | - | chr3.2:80171814-80171833 | MS.gene06421:CDS | 30.0% | |
| ! | TTTCAAACCATTTTCTCTAG+TGG | + | chr3.2:80171870-80171889 | None:intergenic | 30.0% |
| AATGCATTGTGACAAGCATT+TGG | + | chr3.2:80171809-80171828 | None:intergenic | 35.0% | |
| AGAGGACAAATATGAAGAAG+TGG | + | chr3.2:80171624-80171643 | None:intergenic | 35.0% | |
| ATGCATTGTGACAAGCATTT+GGG | + | chr3.2:80171808-80171827 | None:intergenic | 35.0% | |
| ATTCAAAAGGGTTCCTGATT+TGG | - | chr3.2:80171826-80171845 | MS.gene06421:CDS | 35.0% | |
| CAACAACACTACCTTGAAAA+AGG | - | chr3.2:80171661-80171680 | MS.gene06421:CDS | 35.0% | |
| CTTGTCACAATGCATTCAAA+AGG | - | chr3.2:80171813-80171832 | MS.gene06421:CDS | 35.0% | |
| GCACACTATGCATTTGTAAA+TGG | - | chr3.2:80171449-80171468 | MS.gene06421:CDS | 35.0% | |
| TTACAAATGCATAGTGTGCT+AGG | + | chr3.2:80171448-80171467 | None:intergenic | 35.0% | |
| !!! | CTTTTTCAAGGTAGTGTTGT+TGG | + | chr3.2:80171663-80171682 | None:intergenic | 35.0% |
| AATCTTGTACTGAAGCTTGG+AGG | + | chr3.2:80171730-80171749 | None:intergenic | 40.0% | |
| ACAAGCATTTGGGTAGTCTT+TGG | + | chr3.2:80171798-80171817 | None:intergenic | 40.0% | |
| ACTTATCTGCACACTCTTTG+AGG | + | chr3.2:80171688-80171707 | None:intergenic | 40.0% | |
| ATCTTGTACTGAAGCTTGGA+GGG | + | chr3.2:80171729-80171748 | None:intergenic | 40.0% | |
| CCAAATCTTGTACTGAAGCT+TGG | + | chr3.2:80171733-80171752 | None:intergenic | 40.0% | |
| CCAAGCTTCAGTACAAGATT+TGG | - | chr3.2:80171730-80171749 | MS.gene06421:CDS | 40.0% | |
| GCAGATAAGTACAACTATGC+TGG | - | chr3.2:80171698-80171717 | MS.gene06421:CDS | 40.0% | |
| GGATGAGAAATAGTGAGAAG+TGG | + | chr3.2:80171421-80171440 | None:intergenic | 40.0% | |
| GGTTGGAGTAAGAGATTTAG+AGG | + | chr3.2:80171642-80171661 | None:intergenic | 40.0% | |
| ! | GCAAGTGCTTTTGATCAAAG+TGG | + | chr3.2:80171480-80171499 | None:intergenic | 40.0% |
| ! | TTCAAGGTAGTGTTGTTGGT+TGG | + | chr3.2:80171659-80171678 | None:intergenic | 40.0% |
| !! | CTCTTTGAGGACCTTTTTCA+AGG | + | chr3.2:80171675-80171694 | None:intergenic | 40.0% |
| AGTCCAAACGCAGACACAAA+GGG | - | chr3.2:80171554-80171573 | MS.gene06421:CDS | 45.0% | |
| ATGCATAGTGTGCTAGGAAG+AGG | + | chr3.2:80171442-80171461 | None:intergenic | 45.0% | |
| GAGCTTGCCACTAGAGAAAA+TGG | - | chr3.2:80171860-80171879 | MS.gene06421:CDS | 45.0% | |
| TCAGGAGGGTAAACCAAATC+AGG | + | chr3.2:80171842-80171861 | None:intergenic | 45.0% | |
| ! | TTTTCTCTAGTGGCAAGCTC+AGG | + | chr3.2:80171860-80171879 | None:intergenic | 45.0% |
| AAAGGGCCTAGCTGTGATCA+TGG | - | chr3.2:80171571-80171590 | MS.gene06421:CDS | 50.0% | |
| CAGTCCAAACGCAGACACAA+AGG | - | chr3.2:80171553-80171572 | MS.gene06421:CDS | 50.0% | |
| GCCTAGCTGTGATCATGGTT+GGG | - | chr3.2:80171576-80171595 | MS.gene06421:CDS | 50.0% | |
| GCTGTGATCATGGTTGGGAT+TGG | - | chr3.2:80171581-80171600 | MS.gene06421:CDS | 50.0% | |
| TCCCAACCATGATCACAGCT+AGG | + | chr3.2:80171580-80171599 | None:intergenic | 50.0% | |
| TGCGTTTGGACTGCTAGGAT+TGG | + | chr3.2:80171546-80171565 | None:intergenic | 50.0% | |
| AGGCCCTTTGTGTCTGCGTT+TGG | + | chr3.2:80171560-80171579 | None:intergenic | 55.0% | |
| CTCTAGTGGCAAGCTCAGGA+GGG | + | chr3.2:80171856-80171875 | None:intergenic | 55.0% | |
| GGCCTAGCTGTGATCATGGT+TGG | - | chr3.2:80171575-80171594 | MS.gene06421:CDS | 55.0% | |
| GTGTCTGCGTTTGGACTGCT+AGG | + | chr3.2:80171551-80171570 | None:intergenic | 55.0% | |
| TCTCTAGTGGCAAGCTCAGG+AGG | + | chr3.2:80171857-80171876 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 80171404 | 80171936 | 80171404 | ID=MS.gene06421 |
| chr3.2 | mRNA | 80171404 | 80171936 | 80171404 | ID=MS.gene06421.t1;Parent=MS.gene06421 |
| chr3.2 | exon | 80171404 | 80171936 | 80171404 | ID=MS.gene06421.t1.exon1;Parent=MS.gene06421.t1 |
| chr3.2 | CDS | 80171404 | 80171936 | 80171404 | ID=cds.MS.gene06421.t1;Parent=MS.gene06421.t1 |
| Gene Sequence |
| Protein sequence |
