Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065886.t1 | XP_013456625.1 | 95.1 | 123 | 4 | 1 | 1 | 121 | 1 | 123 | 2.60E-55 | 224.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065886.t1 | A0A072UMP2 | 95.1 | 123 | 4 | 1 | 1 | 121 | 1 | 123 | 1.8e-55 | 224.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052493 | MS.gene065886 | 0.800428 | 1.45E-48 | -1.69E-46 |
| MS.gene053094 | MS.gene065886 | 0.867816 | 1.04E-65 | -1.69E-46 |
| MS.gene053963 | MS.gene065886 | 0.827327 | 1.66E-54 | -1.69E-46 |
| MS.gene054222 | MS.gene065886 | 0.815905 | 7.28E-52 | -1.69E-46 |
| MS.gene05439 | MS.gene065886 | 0.826413 | 2.75E-54 | -1.69E-46 |
| MS.gene054479 | MS.gene065886 | 0.814084 | 1.84E-51 | -1.69E-46 |
| MS.gene054576 | MS.gene065886 | 0.814663 | 1.37E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene065886 | 0.873746 | 1.16E-67 | -1.69E-46 |
| MS.gene054893 | MS.gene065886 | 0.869644 | 2.65E-66 | -1.69E-46 |
| MS.gene055147 | MS.gene065886 | 0.868747 | 5.19E-66 | -1.69E-46 |
| MS.gene055148 | MS.gene065886 | 0.850264 | 1.90E-60 | -1.69E-46 |
| MS.gene055149 | MS.gene065886 | 0.839882 | 1.21E-57 | -1.69E-46 |
| MS.gene055226 | MS.gene065886 | 0.829488 | 5.01E-55 | -1.69E-46 |
| MS.gene055827 | MS.gene065886 | 0.838652 | 2.53E-57 | -1.69E-46 |
| MS.gene056699 | MS.gene065886 | 0.802442 | 5.60E-49 | -1.69E-46 |
| MS.gene058429 | MS.gene065886 | 0.804465 | 2.13E-49 | -1.69E-46 |
| MS.gene058592 | MS.gene065886 | 0.804371 | 2.23E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene065886 | 0.816148 | 6.42E-52 | -1.69E-46 |
| MS.gene059686 | MS.gene065886 | 0.800716 | 1.27E-48 | -1.69E-46 |
| MS.gene059774 | MS.gene065886 | 0.837606 | 4.71E-57 | -1.69E-46 |
| MS.gene05985 | MS.gene065886 | -0.801811 | 7.55E-49 | -1.69E-46 |
| MS.gene060012 | MS.gene065886 | 0.804669 | 1.93E-49 | -1.69E-46 |
| MS.gene060412 | MS.gene065886 | 0.814738 | 1.32E-51 | -1.69E-46 |
| MS.gene060413 | MS.gene065886 | 0.836647 | 8.28E-57 | -1.69E-46 |
| MS.gene060697 | MS.gene065886 | 0.835657 | 1.48E-56 | -1.69E-46 |
| MS.gene060840 | MS.gene065886 | 0.803018 | 4.26E-49 | -1.69E-46 |
| MS.gene061047 | MS.gene065886 | 0.810946 | 8.95E-51 | -1.69E-46 |
| MS.gene061188 | MS.gene065886 | 0.813 | 3.19E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065886.t1 | MTR_4g077777 | 95.122 | 123 | 4 | 1 | 1 | 121 | 1 | 123 | 3.23e-82 | 236 |
| MS.gene065886.t1 | MTR_4g111840 | 60.265 | 151 | 28 | 5 | 1 | 121 | 1 | 149 | 2.58e-50 | 157 |
| MS.gene065886.t1 | MTR_0077s0010 | 50.000 | 124 | 53 | 4 | 1 | 117 | 1 | 122 | 1.02e-27 | 99.4 |
| MS.gene065886.t1 | MTR_0077s0010 | 50.000 | 124 | 53 | 4 | 1 | 117 | 1 | 122 | 1.12e-27 | 99.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065886.t1 | AT1G49000 | 53.086 | 81 | 37 | 1 | 42 | 121 | 72 | 152 | 2.09e-23 | 89.0 |
| MS.gene065886.t1 | AT3G18560 | 47.727 | 88 | 34 | 2 | 40 | 115 | 85 | 172 | 4.82e-20 | 81.3 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAACAATATCAGCTATTC+TGG | 0.290432 | 4.2:-32040712 | MS.gene065886:CDS |
| TCTTCTTCATCTTCCCGAAT+TGG | 0.304858 | 4.2:+32040604 | None:intergenic |
| AACAAAGCTTTGTCCCAATT+CGG | 0.318031 | 4.2:-32040618 | MS.gene065886:CDS |
| ATGAATTAAACGGCGGTGTT+TGG | 0.339196 | 4.2:-32040563 | MS.gene065886:CDS |
| CTTCTTCATCTTCCCGAATT+GGG | 0.339635 | 4.2:+32040605 | None:intergenic |
| ACAAAGCTTTGTCCCAATTC+GGG | 0.352806 | 4.2:-32040617 | MS.gene065886:CDS |
| TGCCTTTCCTTTCAAACGGC+TGG | 0.360489 | 4.2:+32040671 | None:intergenic |
| TTATTCATTATGATATTAAC+GGG | 0.416860 | 4.2:-32040488 | MS.gene065886:CDS |
| CATAGAGATAATGAATTAAA+CGG | 0.427132 | 4.2:-32040573 | MS.gene065886:CDS |
| CTACTGTTGGATTGACGCTT+CGG | 0.448546 | 4.2:+32040741 | None:intergenic |
| GTTATTCATTATGATATTAA+CGG | 0.453434 | 4.2:-32040489 | MS.gene065886:CDS |
| TTGGCAGAAGGAGATATTGA+TGG | 0.469704 | 4.2:-32040544 | MS.gene065886:CDS |
| GATATTAACGGGAGACAAAC+CGG | 0.473139 | 4.2:-32040477 | MS.gene065886:CDS |
| TGAATTCAATAACGAGAGAA+AGG | 0.496337 | 4.2:+32040423 | None:intergenic |
| TGTTATGAAGAAGCTACTGT+TGG | 0.497393 | 4.2:+32040728 | None:intergenic |
| CTATTCTGGCTATGCTCTCA+AGG | 0.502283 | 4.2:-32040698 | MS.gene065886:CDS |
| TGGCAGAAGGAGATATTGAT+GGG | 0.520472 | 4.2:-32040543 | MS.gene065886:CDS |
| TGTGAGCCGTTGGATTTCTC+CGG | 0.521477 | 4.2:-32040513 | MS.gene065886:CDS |
| AAACGGCGGTGTTTGGCAGA+AGG | 0.527684 | 4.2:-32040556 | MS.gene065886:CDS |
| AAACCGGTGAAGTTCCTCTC+AGG | 0.529301 | 4.2:-32040461 | MS.gene065886:CDS |
| AGGCAAGCCAGCCGTTTGAA+AGG | 0.530700 | 4.2:-32040678 | MS.gene065886:CDS |
| GATTGACGCTTCGGCGACTC+CGG | 0.537908 | 4.2:+32040750 | None:intergenic |
| GGTGAATCTTACTTCTTCGC+CGG | 0.544106 | 4.2:-32040769 | MS.gene065886:CDS |
| GGGAGGAAAGTGTGAGCCGT+TGG | 0.581512 | 4.2:-32040523 | MS.gene065886:CDS |
| AGCCAGCCGTTTGAAAGGAA+AGG | 0.588702 | 4.2:-32040673 | MS.gene065886:CDS |
| AGACCTGAGAGGAACTTCAC+CGG | 0.627411 | 4.2:+32040458 | None:intergenic |
| CGAGAGAAAGGAATAGCGCG+TGG | 0.637967 | 4.2:+32040435 | None:intergenic |
| AATATCATAATGAATAACGC+CGG | 0.666439 | 4.2:+32040494 | None:intergenic |
| CAGAAGGAGATATTGATGGG+AGG | 0.684772 | 4.2:-32040540 | MS.gene065886:CDS |
| ATAACGCCGGAGAAATCCAA+CGG | 0.714816 | 4.2:+32040507 | None:intergenic |
| ATAGCGCGTGGAGACCTGAG+AGG | 0.722062 | 4.2:+32040447 | None:intergenic |
| AGAGATAATGAATTAAACGG+CGG | 0.734320 | 4.2:-32040570 | MS.gene065886:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTATTCATTATGATATTAA+CGG | - | chr4.2:32040710-32040729 | MS.gene065886:CDS | 15.0% |
| !! | TTATTCATTATGATATTAAC+GGG | - | chr4.2:32040711-32040730 | MS.gene065886:CDS | 15.0% |
| !! | CATAGAGATAATGAATTAAA+CGG | - | chr4.2:32040626-32040645 | MS.gene065886:CDS | 20.0% |
| ! | AATATCATAATGAATAACGC+CGG | + | chr4.2:32040708-32040727 | None:intergenic | 25.0% |
| AGAGATAATGAATTAAACGG+CGG | - | chr4.2:32040629-32040648 | MS.gene065886:CDS | 30.0% | |
| CATAACAATATCAGCTATTC+TGG | - | chr4.2:32040487-32040506 | MS.gene065886:CDS | 30.0% | |
| ! | TTTTTGCCTTTCCTTTCAAA+CGG | + | chr4.2:32040535-32040554 | None:intergenic | 30.0% |
| TGTTATGAAGAAGCTACTGT+TGG | + | chr4.2:32040474-32040493 | None:intergenic | 35.0% | |
| ! | AACAAAGCTTTGTCCCAATT+CGG | - | chr4.2:32040581-32040600 | MS.gene065886:CDS | 35.0% |
| ATGAATTAAACGGCGGTGTT+TGG | - | chr4.2:32040636-32040655 | MS.gene065886:CDS | 40.0% | |
| CTTCTTCATCTTCCCGAATT+GGG | + | chr4.2:32040597-32040616 | None:intergenic | 40.0% | |
| GATATTAACGGGAGACAAAC+CGG | - | chr4.2:32040722-32040741 | MS.gene065886:CDS | 40.0% | |
| TCTTCTTCATCTTCCCGAAT+TGG | + | chr4.2:32040598-32040617 | None:intergenic | 40.0% | |
| TGGCAGAAGGAGATATTGAT+GGG | - | chr4.2:32040656-32040675 | MS.gene065886:CDS | 40.0% | |
| TTGGCAGAAGGAGATATTGA+TGG | - | chr4.2:32040655-32040674 | MS.gene065886:CDS | 40.0% | |
| ! | ACAAAGCTTTGTCCCAATTC+GGG | - | chr4.2:32040582-32040601 | MS.gene065886:CDS | 40.0% |
| ATAACGCCGGAGAAATCCAA+CGG | + | chr4.2:32040695-32040714 | None:intergenic | 45.0% | |
| CAGAAGGAGATATTGATGGG+AGG | - | chr4.2:32040659-32040678 | MS.gene065886:CDS | 45.0% | |
| CTACTGTTGGATTGACGCTT+CGG | + | chr4.2:32040461-32040480 | None:intergenic | 45.0% | |
| GGTGAATCTTACTTCTTCGC+CGG | - | chr4.2:32040430-32040449 | MS.gene065886:CDS | 45.0% | |
| ! | CTATTCTGGCTATGCTCTCA+AGG | - | chr4.2:32040501-32040520 | MS.gene065886:CDS | 45.0% |
| AAACCGGTGAAGTTCCTCTC+AGG | - | chr4.2:32040738-32040757 | MS.gene065886:CDS | 50.0% | |
| AGACCTGAGAGGAACTTCAC+CGG | + | chr4.2:32040744-32040763 | None:intergenic | 50.0% | |
| AGCCAGCCGTTTGAAAGGAA+AGG | - | chr4.2:32040526-32040545 | MS.gene065886:CDS | 50.0% | |
| TGCCTTTCCTTTCAAACGGC+TGG | + | chr4.2:32040531-32040550 | None:intergenic | 50.0% | |
| TGTGAGCCGTTGGATTTCTC+CGG | - | chr4.2:32040686-32040705 | MS.gene065886:CDS | 50.0% | |
| AAACGGCGGTGTTTGGCAGA+AGG | - | chr4.2:32040643-32040662 | MS.gene065886:CDS | 55.0% | |
| AGGCAAGCCAGCCGTTTGAA+AGG | - | chr4.2:32040521-32040540 | MS.gene065886:CDS | 55.0% | |
| CGAGAGAAAGGAATAGCGCG+TGG | + | chr4.2:32040767-32040786 | None:intergenic | 55.0% | |
| ATAGCGCGTGGAGACCTGAG+AGG | + | chr4.2:32040755-32040774 | None:intergenic | 60.0% | |
| GATTGACGCTTCGGCGACTC+CGG | + | chr4.2:32040452-32040471 | None:intergenic | 60.0% | |
| ! | GGGAGGAAAGTGTGAGCCGT+TGG | - | chr4.2:32040676-32040695 | MS.gene065886:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 32040428 | 32040793 | 32040428 | ID=MS.gene065886 |
| chr4.2 | mRNA | 32040428 | 32040793 | 32040428 | ID=MS.gene065886.t1;Parent=MS.gene065886 |
| chr4.2 | exon | 32040428 | 32040793 | 32040428 | ID=MS.gene065886.t1.exon1;Parent=MS.gene065886.t1 |
| chr4.2 | CDS | 32040428 | 32040793 | 32040428 | ID=cds.MS.gene065886.t1;Parent=MS.gene065886.t1 |
| Gene Sequence |
| Protein sequence |
