AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070320


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070320.t1 AT5G22250 64.662 266 90 3 9 270 12 277 6.40e-120 344
MS.gene070320.t1 AT3G44260 61.132 265 98 3 9 270 17 279 4.51e-116 335
MS.gene070320.t1 AT2G32070 50.746 268 127 5 4 270 5 268 4.63e-92 273
MS.gene070320.t1 AT5G10960 50.562 267 128 4 4 270 5 267 7.14e-91 270
MS.gene070320.t1 AT1G80780 49.064 267 132 4 4 270 5 267 1.27e-88 265
MS.gene070320.t1 AT1G80780 49.064 267 132 4 4 270 5 267 1.27e-88 265
MS.gene070320.t1 AT1G80780 49.064 267 132 4 4 270 5 267 1.73e-88 265
MS.gene070320.t1 AT1G15920 47.407 270 134 5 5 269 10 276 8.64e-81 245
MS.gene070320.t1 AT1G15920 47.407 270 134 5 5 269 10 276 8.64e-81 245
MS.gene070320.t1 AT1G61470 37.037 243 130 7 13 249 4 229 1.14e-38 137
MS.gene070320.t1 AT1G06450 33.700 273 157 9 1 268 1 254 1.51e-38 138
MS.gene070320.t1 AT1G27820 35.510 245 143 5 7 248 2 234 8.44e-34 125
MS.gene070320.t1 AT1G27890 35.565 239 139 5 13 248 3 229 1.60e-32 121
MS.gene070320.t1 AT3G44240 35.242 227 132 7 25 249 3 216 3.77e-32 119

Find 62 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCACAGCGCTTATGATTT+TGG 0.104590 4.1:-6049026 MS.gene070320:CDS
ATCAAATTCAACCTCTAAAT+TGG 0.207989 4.1:+6049457 None:intergenic
GATGGCAACCTTCCTCATTT+TGG 0.210731 4.1:-6049254 MS.gene070320:CDS
AAACACGCTGGGGTACTATT+TGG 0.216392 4.1:-6048729 MS.gene070320:CDS
GCTGAATTGATGCTCACTTC+TGG 0.258268 4.1:-6049083 MS.gene070320:CDS
CAATCATCATCCGTCAAGTT+TGG 0.286714 4.1:-6049484 MS.gene070320:CDS
TCTTCAACAAATCAGTTGTT+TGG 0.295934 4.1:-6049055 MS.gene070320:CDS
AACGGTTCGGCAGATTCCTT+CGG 0.352418 4.1:+6048982 None:intergenic
CTTAACGTGGGTCGGGTTGC+TGG 0.360050 4.1:-6048843 MS.gene070320:CDS
AGATGACGGCGGTCAACTTT+GGG 0.368577 4.1:+6049362 None:intergenic
CTTCAACAAATCAGTTGTTT+GGG 0.376564 4.1:-6049054 MS.gene070320:CDS
GGACAAATAGCCGCTACATA+TGG 0.395638 4.1:-6049232 MS.gene070320:CDS
ACGGTTCGGCAGATTCCTTC+GGG 0.395658 4.1:+6048983 None:intergenic
AATCATCATCCGTCAAGTTT+GGG 0.414340 4.1:-6049483 MS.gene070320:CDS
AAGATGACGGCGGTCAACTT+TGG 0.418710 4.1:+6049361 None:intergenic
GAATCTGCCGAACCGTTTAG+AGG 0.420121 4.1:-6048976 MS.gene070320:CDS
AGTTTGGGCATCCAATTTAG+AGG 0.424325 4.1:-6049468 MS.gene070320:CDS
TCCATGGATACTGAATTTCC+AGG 0.437255 4.1:-6049398 MS.gene070320:CDS
GGTTGCCATCACCATCAGAT+AGG 0.449581 4.1:+6049267 None:intergenic
CAAACTTGACGGATGATGAT+TGG 0.450788 4.1:+6049485 None:intergenic
TACTCATTTCTTGAATAATG+AGG 0.477666 4.1:-6048757 MS.gene070320:CDS
TGACCACTATCGTTACTTGA+AGG 0.492905 4.1:-6049330 MS.gene070320:CDS
GACAAATAGCCGCTACATAT+GGG 0.500452 4.1:-6049231 MS.gene070320:CDS
CAATTGAGCCTTGGTTGTAA+AGG 0.504025 4.1:+6049174 None:intergenic
TGCGCTTGAAGTCAATCCCT+TGG 0.505546 4.1:+6049138 None:intergenic
TCAATATCCTTTCATCTCCA+TGG 0.506697 4.1:-6049414 MS.gene070320:CDS
TACAACACTTAACGTGGGTC+GGG 0.508105 4.1:-6048850 MS.gene070320:CDS
AATTGGATGCCCAAACTTGA+CGG 0.510297 4.1:+6049474 None:intergenic
ACCTGGAAATTCAGTATCCA+TGG 0.514112 4.1:+6049397 None:intergenic
AGGTTCTGCAACGCTTTGTA+CGG 0.515634 4.1:-6048891 MS.gene070320:CDS
TTCAAGCGCAATTTATCTCA+TGG 0.520248 4.1:-6049125 MS.gene070320:CDS
CAGATAGGGTGATACCAACT+TGG 0.523809 4.1:+6049282 None:intergenic
GATATTGATTGACAACTTGA+CGG 0.523978 4.1:+6049429 None:intergenic
GCATGCATTTAAGAAAATGA+TGG 0.528476 4.1:-6048781 MS.gene070320:CDS
CTACAACACTTAACGTGGGT+CGG 0.533742 4.1:-6048851 MS.gene070320:CDS
CGGCTATTTGTCCCAAAATG+AGG 0.540450 4.1:+6049242 None:intergenic
GCTGGAAAGTCTCATCAAGC+TGG 0.542113 4.1:-6048825 MS.gene070320:CDS
ATTGACATGCTCTGCCGCCA+AGG 0.544877 4.1:-6049155 MS.gene070320:CDS
AGAGCATGTCAATTGAGCCT+TGG 0.548111 4.1:+6049165 None:intergenic
TTGACATGCTCTGCCGCCAA+GGG 0.557345 4.1:-6049154 MS.gene070320:CDS
GCTTGAAGTCAATCCCTTGG+CGG 0.562753 4.1:+6049141 None:intergenic
GCTCTTAAACTCATCCAAGT+TGG 0.568904 4.1:-6049296 MS.gene070320:CDS
ATGATATGAAACACATGATG+AGG 0.569362 4.1:-6048911 MS.gene070320:CDS
TTGGGTGAATAAATTACACC+TGG 0.570783 4.1:+6049380 None:intergenic
GTTGCCATCACCATCAGATA+GGG 0.583084 4.1:+6049268 None:intergenic
TTCTGCAACGCTTTGTACGG+TGG 0.584961 4.1:-6048888 MS.gene070320:CDS
ATTCAAAATGAACCAGCTGA+AGG 0.587598 4.1:-6049513 None:intergenic
GGTCAGAGGGTTTAAGATGA+CGG 0.592880 4.1:+6049348 None:intergenic
AAGTAACGATAGTGGTCAGA+GGG 0.593594 4.1:+6049335 None:intergenic
TAGTGAAGATATTGACCCGA+AGG 0.595020 4.1:-6048998 MS.gene070320:CDS
TCAGTATCCATGGAGATGAA+AGG 0.598752 4.1:+6049407 None:intergenic
GTTGGTATCACCCTATCTGA+TGG 0.609828 4.1:-6049278 MS.gene070320:CDS
TGACCTTCAAGTAACGATAG+TGG 0.622378 4.1:+6049327 None:intergenic
TATTTGTCCCAAAATGAGGA+AGG 0.623706 4.1:+6049246 None:intergenic
AGTCGCTACAACACTTAACG+TGG 0.625698 4.1:-6048856 MS.gene070320:CDS
CAAGTAACGATAGTGGTCAG+AGG 0.631701 4.1:+6049334 None:intergenic
GAGCATGACCTTTACAACCA+AGG 0.646186 4.1:-6049182 MS.gene070320:CDS
AGTTGAATTCCCATATGTAG+CGG 0.649610 4.1:+6049222 None:intergenic
GATCAGATAGTTTGTTGACG+TGG 0.660381 4.1:-6048803 MS.gene070320:CDS
ATCACCCTATCTGATGGTGA+TGG 0.683961 4.1:-6049272 MS.gene070320:CDS
GTCGCTACAACACTTAACGT+GGG 0.701411 4.1:-6048855 MS.gene070320:CDS
CAGAGGGTTTAAGATGACGG+CGG 0.736962 4.1:+6049351 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAAAAAGTCCTCTAAA+CGG + chr4.1:6049255-6049274 None:intergenic 20.0%
! ATCAAATTCAACCTCTAAAT+TGG + chr4.1:6048762-6048781 None:intergenic 25.0%
! TACTCATTTCTTGAATAATG+AGG - chr4.1:6049459-6049478 MS.gene070320:CDS 25.0%
ATGATATGAAACACATGATG+AGG - chr4.1:6049305-6049324 MS.gene070320:CDS 30.0%
CTTCAACAAATCAGTTGTTT+GGG - chr4.1:6049162-6049181 MS.gene070320:CDS 30.0%
GCATGCATTTAAGAAAATGA+TGG - chr4.1:6049435-6049454 MS.gene070320:CDS 30.0%
TCTTCAACAAATCAGTTGTT+TGG - chr4.1:6049161-6049180 MS.gene070320:CDS 30.0%
! GATATTGATTGACAACTTGA+CGG + chr4.1:6048790-6048809 None:intergenic 30.0%
AAAAAGTCCTCTAAACGGTT+CGG + chr4.1:6049250-6049269 None:intergenic 35.0%
AATCATCATCCGTCAAGTTT+GGG - chr4.1:6048733-6048752 MS.gene070320:CDS 35.0%
AGTTGAATTCCCATATGTAG+CGG + chr4.1:6048997-6049016 None:intergenic 35.0%
TATTTGTCCCAAAATGAGGA+AGG + chr4.1:6048973-6048992 None:intergenic 35.0%
TCAATATCCTTTCATCTCCA+TGG - chr4.1:6048802-6048821 MS.gene070320:CDS 35.0%
TTCAAGCGCAATTTATCTCA+TGG - chr4.1:6049091-6049110 MS.gene070320:CDS 35.0%
TTGGGTGAATAAATTACACC+TGG + chr4.1:6048839-6048858 None:intergenic 35.0%
! TTCACAGCGCTTATGATTTT+GGG - chr4.1:6049191-6049210 MS.gene070320:CDS 35.0%
! TTTCACAGCGCTTATGATTT+TGG - chr4.1:6049190-6049209 MS.gene070320:CDS 35.0%
AATTGGATGCCCAAACTTGA+CGG + chr4.1:6048745-6048764 None:intergenic 40.0%
ACCTGGAAATTCAGTATCCA+TGG + chr4.1:6048822-6048841 None:intergenic 40.0%
AGTTTGGGCATCCAATTTAG+AGG - chr4.1:6048748-6048767 MS.gene070320:CDS 40.0%
CAATCATCATCCGTCAAGTT+TGG - chr4.1:6048732-6048751 MS.gene070320:CDS 40.0%
CAATTGAGCCTTGGTTGTAA+AGG + chr4.1:6049045-6049064 None:intergenic 40.0%
GACAAATAGCCGCTACATAT+GGG - chr4.1:6048985-6049004 MS.gene070320:CDS 40.0%
GCTCTTAAACTCATCCAAGT+TGG - chr4.1:6048920-6048939 MS.gene070320:CDS 40.0%
TAGTGAAGATATTGACCCGA+AGG - chr4.1:6049218-6049237 MS.gene070320:CDS 40.0%
TCAGTATCCATGGAGATGAA+AGG + chr4.1:6048812-6048831 None:intergenic 40.0%
TCCATGGATACTGAATTTCC+AGG - chr4.1:6048818-6048837 MS.gene070320:CDS 40.0%
TGACCACTATCGTTACTTGA+AGG - chr4.1:6048886-6048905 MS.gene070320:CDS 40.0%
TGACCTTCAAGTAACGATAG+TGG + chr4.1:6048892-6048911 None:intergenic 40.0%
! AAGTAACGATAGTGGTCAGA+GGG + chr4.1:6048884-6048903 None:intergenic 40.0%
! ATGGCAACCTTCCTCATTTT+GGG - chr4.1:6048963-6048982 MS.gene070320:CDS 40.0%
! CAAACTTGACGGATGATGAT+TGG + chr4.1:6048734-6048753 None:intergenic 40.0%
!! GATCAGATAGTTTGTTGACG+TGG - chr4.1:6049413-6049432 MS.gene070320:CDS 40.0%
AATGAGGCTCAAAAACACGC+TGG - chr4.1:6049475-6049494 MS.gene070320:CDS 45.0%
AGAGCATGTCAATTGAGCCT+TGG + chr4.1:6049054-6049073 None:intergenic 45.0%
AGGTTCTGCAACGCTTTGTA+CGG - chr4.1:6049325-6049344 MS.gene070320:CDS 45.0%
AGTCGCTACAACACTTAACG+TGG - chr4.1:6049360-6049379 MS.gene070320:CDS 45.0%
ATGAGGCTCAAAAACACGCT+GGG - chr4.1:6049476-6049495 MS.gene070320:CDS 45.0%
CAGATAGGGTGATACCAACT+TGG + chr4.1:6048937-6048956 None:intergenic 45.0%
CGGCTATTTGTCCCAAAATG+AGG + chr4.1:6048977-6048996 None:intergenic 45.0%
CTACAACACTTAACGTGGGT+CGG - chr4.1:6049365-6049384 MS.gene070320:CDS 45.0%
GAGCATGACCTTTACAACCA+AGG - chr4.1:6049034-6049053 MS.gene070320:CDS 45.0%
GCTGAATTGATGCTCACTTC+TGG - chr4.1:6049133-6049152 MS.gene070320:CDS 45.0%
GGACAAATAGCCGCTACATA+TGG - chr4.1:6048984-6049003 MS.gene070320:CDS 45.0%
GTCGCTACAACACTTAACGT+GGG - chr4.1:6049361-6049380 MS.gene070320:CDS 45.0%
GTTGCCATCACCATCAGATA+GGG + chr4.1:6048951-6048970 None:intergenic 45.0%
GTTGGTATCACCCTATCTGA+TGG - chr4.1:6048938-6048957 MS.gene070320:CDS 45.0%
TACAACACTTAACGTGGGTC+GGG - chr4.1:6049366-6049385 MS.gene070320:CDS 45.0%
! AAACACGCTGGGGTACTATT+TGG - chr4.1:6049487-6049506 MS.gene070320:CDS 45.0%
! ATCACCCTATCTGATGGTGA+TGG - chr4.1:6048944-6048963 MS.gene070320:CDS 45.0%
! CAAGTAACGATAGTGGTCAG+AGG + chr4.1:6048885-6048904 None:intergenic 45.0%
! GATGGCAACCTTCCTCATTT+TGG - chr4.1:6048962-6048981 MS.gene070320:CDS 45.0%
! GGTCAGAGGGTTTAAGATGA+CGG + chr4.1:6048871-6048890 None:intergenic 45.0%
!!! TGATTGGCTTTTCCTTCAGC+TGG + chr4.1:6048718-6048737 None:intergenic 45.0%
!!! AATATTTTAACAATATTATT+TGG - chr4.1:6049271-6049290 MS.gene070320:CDS 5.0%
GAATCTGCCGAACCGTTTAG+AGG - chr4.1:6049240-6049259 MS.gene070320:CDS 50.0%
GCTGGAAAGTCTCATCAAGC+TGG - chr4.1:6049391-6049410 MS.gene070320:CDS 50.0%
GCTTGAAGTCAATCCCTTGG+CGG + chr4.1:6049078-6049097 None:intergenic 50.0%
GGTTGCCATCACCATCAGAT+AGG + chr4.1:6048952-6048971 None:intergenic 50.0%
TGAGGCTCAAAAACACGCTG+GGG - chr4.1:6049477-6049496 MS.gene070320:CDS 50.0%
TGCGCTTGAAGTCAATCCCT+TGG + chr4.1:6049081-6049100 None:intergenic 50.0%
TTCTGCAACGCTTTGTACGG+TGG - chr4.1:6049328-6049347 MS.gene070320:CDS 50.0%
! AACGGTTCGGCAGATTCCTT+CGG + chr4.1:6049237-6049256 None:intergenic 50.0%
! AAGATGACGGCGGTCAACTT+TGG + chr4.1:6048858-6048877 None:intergenic 50.0%
! AGATGACGGCGGTCAACTTT+GGG + chr4.1:6048857-6048876 None:intergenic 50.0%
! CAGAGGGTTTAAGATGACGG+CGG + chr4.1:6048868-6048887 None:intergenic 50.0%
ATTGACATGCTCTGCCGCCA+AGG - chr4.1:6049061-6049080 MS.gene070320:CDS 55.0%
TTGACATGCTCTGCCGCCAA+GGG - chr4.1:6049062-6049081 MS.gene070320:CDS 55.0%
! ACGGTTCGGCAGATTCCTTC+GGG + chr4.1:6049236-6049255 None:intergenic 55.0%
! CTTAACGTGGGTCGGGTTGC+TGG - chr4.1:6049373-6049392 MS.gene070320:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 6048710 6049528 6048710 ID=MS.gene070320
chr4.1 mRNA 6048710 6049528 6048710 ID=MS.gene070320.t1;Parent=MS.gene070320
chr4.1 exon 6048710 6049528 6048710 ID=MS.gene070320.t1.exon1;Parent=MS.gene070320.t1
chr4.1 CDS 6048710 6049528 6048710 ID=cds.MS.gene070320.t1;Parent=MS.gene070320.t1
Gene Sequence

>MS.gene070320

ATGAACCAGCTGAAGGAAAAGCCAATCATCATCCGTCAAGTTTGGGCATCCAATTTAGAGGTTGAATTTGATCTCATCCGTCAAGTTGTCAATCAATATCCTTTCATCTCCATGGATACTGAATTTCCAGGTGTAATTTATTCACCCAAAGTTGACCGCCGTCATCTTAAACCCTCTGACCACTATCGTTACTTGAAGGTCAACGTTGATGCTCTTAAACTCATCCAAGTTGGTATCACCCTATCTGATGGTGATGGCAACCTTCCTCATTTTGGGACAAATAGCCGCTACATATGGGAATTCAACTTTTGCGATTTTGATTTTGAGCATGACCTTTACAACCAAGGCTCAATTGACATGCTCTGCCGCCAAGGGATTGACTTCAAGCGCAATTTATCTCATGGTGTGAATTCGTCACGTTTTGCTGAATTGATGCTCACTTCTGGACTTGTCTTCAACAAATCAGTTGTTTGGGTCACATTTCACAGCGCTTATGATTTTGGGTATTTAGTGAAGATATTGACCCGAAGGAATCTGCCGAACCGTTTAGAGGACTTTTTAAATATTTTAACAATATTATTTGGAAAGAATGTTTATGATATGAAACACATGATGAGGTTCTGCAACGCTTTGTACGGTGGTCTCGAGCGAGTCGCTACAACACTTAACGTGGGTCGGGTTGCTGGAAAGTCTCATCAAGCTGGATCAGATAGTTTGTTGACGTGGCATGCATTTAAGAAAATGATGGATACTCATTTCTTGAATAATGAGGCTCAAAAACACGCTGGGGTACTATTTGGATTAGAAATTGCAACTTAG

Protein sequence

>MS.gene070320.t1

MNQLKEKPIIIRQVWASNLEVEFDLIRQVVNQYPFISMDTEFPGVIYSPKVDRRHLKPSDHYRYLKVNVDALKLIQVGITLSDGDGNLPHFGTNSRYIWEFNFCDFDFEHDLYNQGSIDMLCRQGIDFKRNLSHGVNSSRFAELMLTSGLVFNKSVVWVTFHSAYDFGYLVKILTRRNLPNRLEDFLNILTILFGKNVYDMKHMMRFCNALYGGLERVATTLNVGRVAGKSHQAGSDSLLTWHAFKKMMDTHFLNNEAQKHAGVLFGLEIAT