AlfalfaGEDB | MS.gene071569

M. sativa cultivar XinJiangDaYe / MS.gene071569

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071569.t1	XP_013447314.1	92.1	114	9	0	1	114	23	136	3.40E-50	207.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071569.t1	I3S9W1	92.1	114	9	0	1	114	23	136	2.4e-50	207.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04919	MS.gene071569	0.808336	3.26E-50	-1.69E-46
MS.gene04920	MS.gene071569	0.826754	2.28E-54	-1.69E-46
MS.gene049283	MS.gene071569	0.843701	1.19E-58	-1.69E-46
MS.gene049499	MS.gene071569	0.84272	2.18E-58	-1.69E-46
MS.gene049983	MS.gene071569	0.828743	7.59E-55	-1.69E-46
MS.gene051699	MS.gene071569	0.837252	5.80E-57	-1.69E-46
MS.gene052118	MS.gene071569	0.822248	2.62E-53	-1.69E-46
MS.gene052685	MS.gene071569	0.814205	1.73E-51	-1.69E-46
MS.gene053963	MS.gene071569	0.837372	5.40E-57	-1.69E-46
MS.gene054222	MS.gene071569	0.809541	1.80E-50	-1.69E-46
MS.gene054684	MS.gene071569	0.801001	1.11E-48	-1.69E-46
MS.gene054892	MS.gene071569	0.832701	8.14E-56	-1.69E-46
MS.gene054893	MS.gene071569	0.842932	1.91E-58	-1.69E-46
MS.gene054957	MS.gene071569	0.809264	2.06E-50	-1.69E-46
MS.gene055150	MS.gene071569	0.821025	5.03E-53	-1.69E-46
MS.gene055724	MS.gene071569	0.822209	2.68E-53	-1.69E-46
MS.gene05624	MS.gene071569	0.834097	3.65E-56	-1.69E-46
MS.gene056677	MS.gene071569	0.809272	2.05E-50	-1.69E-46
MS.gene056699	MS.gene071569	0.810627	1.05E-50	-1.69E-46
MS.gene05686	MS.gene071569	0.812332	4.47E-51	-1.69E-46
MS.gene057102	MS.gene071569	0.801188	1.01E-48	-1.69E-46
MS.gene057155	MS.gene071569	0.801768	7.71E-49	-1.69E-46
MS.gene057622	MS.gene071569	0.806068	9.84E-50	-1.69E-46
MS.gene058095	MS.gene071569	0.803909	2.78E-49	-1.69E-46
MS.gene058159	MS.gene071569	0.838369	3.00E-57	-1.69E-46
MS.gene058279	MS.gene071569	0.801268	9.76E-49	-1.69E-46
MS.gene058429	MS.gene071569	0.805581	1.25E-49	-1.69E-46
MS.gene058592	MS.gene071569	0.82485	6.46E-54	-1.69E-46
MS.gene058781	MS.gene071569	0.806662	7.38E-50	-1.69E-46
MS.gene05878	MS.gene071569	0.889318	2.70E-73	-1.69E-46
MS.gene05952	MS.gene071569	0.959826	7.15E-118	-1.69E-46
MS.gene05956	MS.gene071569	0.874375	7.09E-68	-1.69E-46
MS.gene059686	MS.gene071569	0.818895	1.54E-52	-1.69E-46
MS.gene05985	MS.gene071569	-0.834868	2.34E-56	-1.69E-46
MS.gene059901	MS.gene071569	-0.806207	9.20E-50	-1.69E-46
MS.gene060012	MS.gene071569	0.831309	1.80E-55	-1.69E-46
MS.gene06023	MS.gene071569	0.803911	2.78E-49	-1.69E-46
MS.gene060412	MS.gene071569	0.800552	1.37E-48	-1.69E-46
MS.gene060840	MS.gene071569	0.800019	1.75E-48	-1.69E-46
MS.gene060933	MS.gene071569	0.812861	3.43E-51	-1.69E-46
MS.gene060935	MS.gene071569	0.816826	4.53E-52	-1.69E-46
MS.gene060937	MS.gene071569	0.833967	3.93E-56	-1.69E-46
MS.gene061243	MS.gene071569	0.804849	1.77E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071569.t1	MTR_8g102755	92.105	114	9	0	1	114	23	136	2.56e-74	220

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 21 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGACAGAAACAAAGAAATT+TGG	0.303541	4.3:-2520434	MS.gene071569:CDS
ATTGAAGATGATGCTACTTT+TGG	0.318948	4.3:-2520489	None:intergenic
CAAAGCTTTCATGATGAAAA+TGG	0.325691	4.3:-2520167	MS.gene071569:CDS
ATTCAAATCAAGCTTGTTGC+TGG	0.398775	4.3:-2520410	MS.gene071569:CDS
ATCTATTACACCATTATTTG+CGG	0.399079	4.3:+2520196	None:intergenic
GGATGTGGACATAAATTGTT+TGG	0.414508	4.3:-2520389	MS.gene071569:CDS
GAATCTACCTTTCCTGAGTC+AGG	0.424703	4.3:+2520326	None:intergenic
ATCAAGCTTGTTGCTGGATG+TGG	0.461106	4.3:-2520404	MS.gene071569:CDS
GTGGAGAGTGCCGCAAATAA+TGG	0.466076	4.3:-2520206	MS.gene071569:CDS
CATAAATTGTTTGGACGGAA+AGG	0.490271	4.3:-2520380	MS.gene071569:CDS
TCAGGAAAAGCTCTTTCATC+GGG	0.511579	4.3:+2520344	None:intergenic
ATAAATTGTTTGGACGGAAA+GGG	0.528425	4.3:-2520379	MS.gene071569:CDS
GTCAGGAAAAGCTCTTTCAT+CGG	0.531583	4.3:+2520343	None:intergenic
TCAGGAAAGGTAGATTCTGT+TGG	0.537763	4.3:-2520320	MS.gene071569:CDS
GTGGACATAAATTGTTTGGA+CGG	0.566125	4.3:-2520385	MS.gene071569:CDS
ACAACTTGGAGTTGAAGAGA+AGG	0.600247	4.3:-2520252	MS.gene071569:CDS
CAACTTGGAGTTGAAGAGAA+GGG	0.601023	4.3:-2520251	MS.gene071569:CDS
CTTGATTTGATATAACAACC+AGG	0.620765	4.3:+2520457	None:intergenic
TGAAAATGGTGTTCGCCTGA+CGG	0.637093	4.3:-2520153	MS.gene071569:CDS
AAATAATGGTGTAATAGATG+AGG	0.639630	4.3:-2520192	MS.gene071569:CDS
AAATGGTGTTCGCCTGACGG+TGG	0.727191	4.3:-2520150	MS.gene071569:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAATAATGGTGTAATAGATG+AGG	-	chr4.3:2520411-2520430	MS.gene071569:CDS	25.0%
!	AAGACAGAAACAAAGAAATT+TGG	-	chr4.3:2520169-2520188	MS.gene071569:CDS	25.0%
!	ATCTATTACACCATTATTTG+CGG	+	chr4.3:2520410-2520429	None:intergenic	25.0%
!!	AAAGAAAAAGAAGTACAACT+TGG	-	chr4.3:2520337-2520356	MS.gene071569:CDS	25.0%
	AAACAAGATGCAGAAAAATG+AGG	-	chr4.3:2520375-2520394	MS.gene071569:CDS	30.0%
	ATAAATTGTTTGGACGGAAA+GGG	-	chr4.3:2520224-2520243	MS.gene071569:CDS	30.0%
	CAAAGCTTTCATGATGAAAA+TGG	-	chr4.3:2520436-2520455	MS.gene071569:CDS	30.0%
!	CATTTTCATCATGAAAGCTT+TGG	+	chr4.3:2520438-2520457	None:intergenic	30.0%
!	CTTGATTTGATATAACAACC+AGG	+	chr4.3:2520149-2520168	None:intergenic	30.0%
	CATAAATTGTTTGGACGGAA+AGG	-	chr4.3:2520223-2520242	MS.gene071569:CDS	35.0%
	GGATGTGGACATAAATTGTT+TGG	-	chr4.3:2520214-2520233	MS.gene071569:CDS	35.0%
	GTGGACATAAATTGTTTGGA+CGG	-	chr4.3:2520218-2520237	MS.gene071569:CDS	35.0%
!!	ATTCAAATCAAGCTTGTTGC+TGG	-	chr4.3:2520193-2520212	MS.gene071569:CDS	35.0%
	ACAACTTGGAGTTGAAGAGA+AGG	-	chr4.3:2520351-2520370	MS.gene071569:CDS	40.0%
	CAACTTGGAGTTGAAGAGAA+GGG	-	chr4.3:2520352-2520371	MS.gene071569:CDS	40.0%
	CAAGATGCAGAAAAATGAGG+TGG	-	chr4.3:2520378-2520397	MS.gene071569:CDS	40.0%
	GTCAGGAAAAGCTCTTTCAT+CGG	+	chr4.3:2520263-2520282	None:intergenic	40.0%
	TCAGGAAAAGCTCTTTCATC+GGG	+	chr4.3:2520262-2520281	None:intergenic	40.0%
	TCAGGAAAGGTAGATTCTGT+TGG	-	chr4.3:2520283-2520302	MS.gene071569:CDS	40.0%
	GAATCTACCTTTCCTGAGTC+AGG	+	chr4.3:2520280-2520299	None:intergenic	45.0%
	TGAAAATGGTGTTCGCCTGA+CGG	-	chr4.3:2520450-2520469	MS.gene071569:CDS	45.0%
!	AGCTTTTCCTGACTCAGGAA+AGG	-	chr4.3:2520270-2520289	MS.gene071569:CDS	45.0%
!	ATGTTTTCTTCACCACCGTC+AGG	+	chr4.3:2520468-2520487	None:intergenic	45.0%
!	GAAAGAGCTTTTCCTGACTC+AGG	-	chr4.3:2520265-2520284	MS.gene071569:CDS	45.0%
!!	ATCAAGCTTGTTGCTGGATG+TGG	-	chr4.3:2520199-2520218	MS.gene071569:CDS	45.0%
!!	TACTTTTGGCTGTTTGTGCC+TGG	-	chr4.3:2520128-2520147	MS.gene071569:CDS	45.0%
!!	GTGGAGAGTGCCGCAAATAA+TGG	-	chr4.3:2520397-2520416	MS.gene071569:CDS	50.0%
	AAATGGTGTTCGCCTGACGG+TGG	-	chr4.3:2520453-2520472	MS.gene071569:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	2520121	2520504	2520121	ID=MS.gene071569
chr4.3	mRNA	2520121	2520504	2520121	ID=MS.gene071569.t1;Parent=MS.gene071569
chr4.3	exon	2520121	2520504	2520121	ID=MS.gene071569.t1.exon1;Parent=MS.gene071569.t1
chr4.3	CDS	2520121	2520504	2520121	ID=cds.MS.gene071569.t1;Parent=MS.gene071569.t1

Gene Sequence

>MS.gene071569

ATGATGCTACTTTTGGCTGTTTGTGCCTGGTTGTTATATCAAATCAAGAAGACAGAAACAAAGAAATTTGGTATTCAAATCAAGCTTGTTGCTGGATGTGGACATAAATTGTTTGGACGGAAAGGGATGCTATCACGTCCCGATGAAAGAGCTTTTCCTGACTCAGGAAAGGTAGATTCTGTTGGAGAAGTAACAAATGAGTTTAAAAGTAATGACAAAGAAAAAGAAGTACAACTTGGAGTTGAAGAGAAGGGAAACAAGATGCAGAAAAATGAGGTGGAGAGTGCCGCAAATAATGGTGTAATAGATGAGGTCCAAAGCTTTCATGATGAAAATGGTGTTCGCCTGACGGTGGTGAAGAAAACATGTCAACTTTTAGAATAG

Protein sequence

>MS.gene071569.t1

MMLLLAVCAWLLYQIKKTETKKFGIQIKLVAGCGHKLFGRKGMLSRPDERAFPDSGKVDSVGEVTNEFKSNDKEKEVQLGVEEKGNKMQKNEVESAANNGVIDEVQSFHDENGVRLTVVKKTCQLLE