Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071685.t1 | XP_013454503.1 | 95.8 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 1.40E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071685.t1 | Q9SUE0 | 86.2 | 87 | 12 | 0 | 7 | 93 | 127 | 213 | 3.0e-39 | 162.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071685.t1 | A0A072UFK1 | 95.8 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 9.8e-44 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049805 | MS.gene071685 | 0.80131 | 9.56E-49 | -1.69E-46 |
| MS.gene050163 | MS.gene071685 | 0.809484 | 1.85E-50 | -1.69E-46 |
| MS.gene050333 | MS.gene071685 | 0.801744 | 7.79E-49 | -1.69E-46 |
| MS.gene050767 | MS.gene071685 | 0.821617 | 3.67E-53 | -1.69E-46 |
| MS.gene050770 | MS.gene071685 | 0.824731 | 6.89E-54 | -1.69E-46 |
| MS.gene050812 | MS.gene071685 | 0.80462 | 1.98E-49 | -1.69E-46 |
| MS.gene051228 | MS.gene071685 | 0.818661 | 1.74E-52 | -1.69E-46 |
| MS.gene051666 | MS.gene071685 | 0.811553 | 6.61E-51 | -1.69E-46 |
| MS.gene052290 | MS.gene071685 | 0.813252 | 2.81E-51 | -1.69E-46 |
| MS.gene052322 | MS.gene071685 | 0.814677 | 1.36E-51 | -1.69E-46 |
| MS.gene052379 | MS.gene071685 | 0.802824 | 4.67E-49 | -1.69E-46 |
| MS.gene053009 | MS.gene071685 | 0.824572 | 7.51E-54 | -1.69E-46 |
| MS.gene053284 | MS.gene071685 | 0.917916 | 2.96E-86 | -1.69E-46 |
| MS.gene053285 | MS.gene071685 | 0.938377 | 7.44E-99 | -1.69E-46 |
| MS.gene053322 | MS.gene071685 | 0.805196 | 1.50E-49 | -1.69E-46 |
| MS.gene053632 | MS.gene071685 | 0.802258 | 6.11E-49 | -1.69E-46 |
| MS.gene053916 | MS.gene071685 | 0.803336 | 3.66E-49 | -1.69E-46 |
| MS.gene054376 | MS.gene071685 | 0.807323 | 5.35E-50 | -1.69E-46 |
| MS.gene055254 | MS.gene071685 | 0.802152 | 6.43E-49 | -1.69E-46 |
| MS.gene055397 | MS.gene071685 | 0.806444 | 8.20E-50 | -1.69E-46 |
| MS.gene055412 | MS.gene071685 | 0.80591 | 1.06E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene071685 | 0.802456 | 5.56E-49 | -1.69E-46 |
| MS.gene056719 | MS.gene071685 | 0.811391 | 7.17E-51 | -1.69E-46 |
| MS.gene056796 | MS.gene071685 | 0.812604 | 3.90E-51 | -1.69E-46 |
| MS.gene056897 | MS.gene071685 | 0.827339 | 1.65E-54 | -1.69E-46 |
| MS.gene056903 | MS.gene071685 | 0.802741 | 4.86E-49 | -1.69E-46 |
| MS.gene057129 | MS.gene071685 | 0.849337 | 3.45E-60 | -1.69E-46 |
| MS.gene057295 | MS.gene071685 | 0.827819 | 1.27E-54 | -1.69E-46 |
| MS.gene058082 | MS.gene071685 | 0.82584 | 3.76E-54 | -1.69E-46 |
| MS.gene058293 | MS.gene071685 | 0.826897 | 2.11E-54 | -1.69E-46 |
| MS.gene058748 | MS.gene071685 | 0.810296 | 1.24E-50 | -1.69E-46 |
| MS.gene058787 | MS.gene071685 | 0.821854 | 3.24E-53 | -1.69E-46 |
| MS.gene058993 | MS.gene071685 | 0.801683 | 8.02E-49 | -1.69E-46 |
| MS.gene05959 | MS.gene071685 | 0.817601 | 3.03E-52 | -1.69E-46 |
| MS.gene059676 | MS.gene071685 | 0.810016 | 1.42E-50 | -1.69E-46 |
| MS.gene059960 | MS.gene071685 | 0.828401 | 9.18E-55 | -1.69E-46 |
| MS.gene06111 | MS.gene071685 | 0.818288 | 2.12E-52 | -1.69E-46 |
| MS.gene061150 | MS.gene071685 | 0.833725 | 4.52E-56 | -1.69E-46 |
| MS.gene061200 | MS.gene071685 | 0.819535 | 1.10E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071685.t1 | MTR_4g005520 | 95.789 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 4.30e-62 | 187 |
| MS.gene071685.t1 | MTR_3g105100 | 47.368 | 76 | 35 | 3 | 15 | 89 | 120 | 191 | 2.63e-14 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071685.t1 | AT4G27990 | 85.227 | 88 | 13 | 0 | 7 | 94 | 127 | 214 | 8.55e-52 | 162 |
| MS.gene071685.t1 | AT3G07430 | 83.908 | 87 | 14 | 0 | 7 | 93 | 141 | 227 | 5.25e-51 | 160 |
| MS.gene071685.t1 | AT5G21920 | 50.000 | 76 | 33 | 3 | 15 | 89 | 133 | 204 | 8.24e-15 | 67.4 |
Find 24 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAACCATTGTCTGCAATT+AGG | 0.283838 | 4.3:+88875786 | MS.gene071685:CDS |
| ACAACCATTGTCTGCAATTA+GGG | 0.307081 | 4.3:+88875787 | MS.gene071685:CDS |
| ACAGTGGTGTGTTGTTGGTT+AGG | 0.327645 | 4.3:+88875726 | MS.gene071685:CDS |
| ATCCTTATCTAAGTTTGTTT+AGG | 0.329465 | 4.3:+88875819 | MS.gene071685:CDS |
| CAGTGGTGTGTTGTTGGTTA+GGG | 0.363509 | 4.3:+88875727 | MS.gene071685:CDS |
| TAGCGGCGGGGTTGAAGAAA+TGG | 0.403666 | 4.3:+88875693 | MS.gene071685:CDS |
| TATTTACAGTGGTGTGTTGT+TGG | 0.416695 | 4.3:+88875721 | MS.gene071685:CDS |
| GACGGTGAACACGCCGTTGA+CGG | 0.439588 | 4.3:+88875667 | MS.gene071685:CDS |
| CCGTTGACGGTTGTAGCGGC+GGG | 0.441420 | 4.3:+88875680 | MS.gene071685:CDS |
| TGTCTTTCCCATGGAATGTT+GGG | 0.454889 | 4.3:-88875767 | None:intergenic |
| GTTGGTTTCCCAACATTCCA+TGG | 0.512936 | 4.3:+88875759 | MS.gene071685:CDS |
| GACAATGGTTGTCTTTCCCA+TGG | 0.515980 | 4.3:-88875776 | None:intergenic |
| TTCCTAAACAAACTTAGATA+AGG | 0.528049 | 4.3:-88875821 | None:intergenic |
| GCCGTTGACGGTTGTAGCGG+CGG | 0.535668 | 4.3:+88875679 | MS.gene071685:CDS |
| TTGGTTTCCCAACATTCCAT+GGG | 0.548737 | 4.3:+88875760 | MS.gene071685:CDS |
| CCCGCCGCTACAACCGTCAA+CGG | 0.573983 | 4.3:-88875680 | None:intergenic |
| TTGTCTTTCCCATGGAATGT+TGG | 0.574451 | 4.3:-88875768 | None:intergenic |
| AAAACAGCAAAAGCAAGAAG+AGG | 0.574512 | 4.3:-88875881 | None:intergenic |
| CACGCCGTTGACGGTTGTAG+CGG | 0.575214 | 4.3:+88875676 | MS.gene071685:CDS |
| CGTTGACGGTTGTAGCGGCG+GGG | 0.610796 | 4.3:+88875681 | MS.gene071685:CDS |
| GGCGGGGTTGAAGAAATGGT+TGG | 0.623671 | 4.3:+88875697 | MS.gene071685:CDS |
| AAATGGTTGGATATTTACAG+TGG | 0.642279 | 4.3:+88875710 | MS.gene071685:CDS |
| AGATCCCTAATTGCAGACAA+TGG | 0.662621 | 4.3:-88875791 | None:intergenic |
| GGATCTAAACAGGAACAGTG+AGG | 0.722011 | 4.3:-88875922 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCCTAAACAAACTTAGATA+AGG | - | chr4.3:88875824-88875843 | None:intergenic | 25.0% |
| !! | ATCCTTATCTAAGTTTGTTT+AGG | + | chr4.3:88875819-88875838 | MS.gene071685:CDS | 25.0% |
| !!! | ATATCAAGAGTATCAAAAAC+AGG | - | chr4.3:88875857-88875876 | None:intergenic | 25.0% |
| AAATGGTTGGATATTTACAG+TGG | + | chr4.3:88875710-88875729 | MS.gene071685:CDS | 30.0% | |
| AAAACAGCAAAAGCAAGAAG+AGG | - | chr4.3:88875884-88875903 | None:intergenic | 35.0% | |
| ACAACCATTGTCTGCAATTA+GGG | + | chr4.3:88875787-88875806 | MS.gene071685:CDS | 35.0% | |
| TATTTACAGTGGTGTGTTGT+TGG | + | chr4.3:88875721-88875740 | MS.gene071685:CDS | 35.0% | |
| !! | TCAAGAGTATCAAAAACAGG+TGG | - | chr4.3:88875854-88875873 | None:intergenic | 35.0% |
| !!! | CTTCTTGCTTTTGCTGTTTT+GGG | + | chr4.3:88875884-88875903 | MS.gene071685:CDS | 35.0% |
| !!! | TCTTCTTGCTTTTGCTGTTT+TGG | + | chr4.3:88875883-88875902 | MS.gene071685:CDS | 35.0% |
| AGATCCCTAATTGCAGACAA+TGG | - | chr4.3:88875794-88875813 | None:intergenic | 40.0% | |
| GACAACCATTGTCTGCAATT+AGG | + | chr4.3:88875786-88875805 | MS.gene071685:CDS | 40.0% | |
| TGTCTTTCCCATGGAATGTT+GGG | - | chr4.3:88875770-88875789 | None:intergenic | 40.0% | |
| TTGGTTTCCCAACATTCCAT+GGG | + | chr4.3:88875760-88875779 | MS.gene071685:CDS | 40.0% | |
| TTGTCTTTCCCATGGAATGT+TGG | - | chr4.3:88875771-88875790 | None:intergenic | 40.0% | |
| !!! | TGGTTAGGGTTTTGTTGAGT+TGG | + | chr4.3:88875741-88875760 | MS.gene071685:CDS | 40.0% |
| GACAATGGTTGTCTTTCCCA+TGG | - | chr4.3:88875779-88875798 | None:intergenic | 45.0% | |
| ! | ACAGTGGTGTGTTGTTGGTT+AGG | + | chr4.3:88875726-88875745 | MS.gene071685:CDS | 45.0% |
| ! | CAGTGGTGTGTTGTTGGTTA+GGG | + | chr4.3:88875727-88875746 | MS.gene071685:CDS | 45.0% |
| ! | GTTGGTTTCCCAACATTCCA+TGG | + | chr4.3:88875759-88875778 | MS.gene071685:CDS | 45.0% |
| !! | CCCGTCCTAGTCTTTTTCAT+AGG | - | chr4.3:88875647-88875666 | None:intergenic | 45.0% |
| GAAAAAGACTAGGACGGGGA+CGG | + | chr4.3:88875649-88875668 | MS.gene071685:CDS | 50.0% | |
| !! | GCTGTTTTGGGTACTCTTGC+TGG | + | chr4.3:88875896-88875915 | MS.gene071685:CDS | 50.0% |
| GGCGGGGTTGAAGAAATGGT+TGG | + | chr4.3:88875697-88875716 | MS.gene071685:CDS | 55.0% | |
| TAGCGGCGGGGTTGAAGAAA+TGG | + | chr4.3:88875693-88875712 | MS.gene071685:CDS | 55.0% | |
| CACGCCGTTGACGGTTGTAG+CGG | + | chr4.3:88875676-88875695 | MS.gene071685:CDS | 60.0% | |
| GACGGTGAACACGCCGTTGA+CGG | + | chr4.3:88875667-88875686 | MS.gene071685:CDS | 60.0% | |
| CCCGCCGCTACAACCGTCAA+CGG | - | chr4.3:88875683-88875702 | None:intergenic | 65.0% | |
| CCGTTGACGGTTGTAGCGGC+GGG | + | chr4.3:88875680-88875699 | MS.gene071685:CDS | 65.0% | |
| CGTTGACGGTTGTAGCGGCG+GGG | + | chr4.3:88875681-88875700 | MS.gene071685:CDS | 65.0% | |
| GCCGTTGACGGTTGTAGCGG+CGG | + | chr4.3:88875679-88875698 | MS.gene071685:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 88875647 | 88875934 | 88875647 | ID=MS.gene071685 |
| chr4.3 | mRNA | 88875647 | 88875934 | 88875647 | ID=MS.gene071685.t1;Parent=MS.gene071685 |
| chr4.3 | exon | 88875647 | 88875934 | 88875647 | ID=MS.gene071685.t1.exon1;Parent=MS.gene071685.t1 |
| chr4.3 | CDS | 88875647 | 88875934 | 88875647 | ID=cds.MS.gene071685.t1;Parent=MS.gene071685.t1 |
| Gene Sequence |
| Protein sequence |
