AlfalfaGEDB | MS.gene21665

M. sativa cultivar XinJiangDaYe / MS.gene21665

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21665.t1	XP_003610991.1	91.2	148	7	1	1	148	1	142	1.70E-64	255.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21665.t1	B7FME5	91.2	148	7	1	1	148	1	142	1.2e-64	255.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049993	MS.gene21665	0.846984	1.54E-59	-1.69E-46
MS.gene050168	MS.gene21665	0.819358	1.21E-52	-1.69E-46
MS.gene051525	MS.gene21665	0.831463	1.65E-55	-1.69E-46
MS.gene051582	MS.gene21665	0.808518	2.98E-50	-1.69E-46
MS.gene052011	MS.gene21665	-0.850271	1.89E-60	-1.69E-46
MS.gene052253	MS.gene21665	0.824438	8.07E-54	-1.69E-46
MS.gene052589	MS.gene21665	0.8043	2.31E-49	-1.69E-46
MS.gene053330	MS.gene21665	0.803074	4.15E-49	-1.69E-46
MS.gene053411	MS.gene21665	0.800675	1.29E-48	-1.69E-46
MS.gene053836	MS.gene21665	0.807995	3.85E-50	-1.69E-46
MS.gene053873	MS.gene21665	0.816219	6.19E-52	-1.69E-46
MS.gene054102	MS.gene21665	0.85877	6.56E-63	-1.69E-46
MS.gene054108	MS.gene21665	0.833505	5.13E-56	-1.69E-46
MS.gene054110	MS.gene21665	0.816203	6.24E-52	-1.69E-46
MS.gene05413	MS.gene21665	0.829486	5.01E-55	-1.69E-46
MS.gene054183	MS.gene21665	0.859376	4.32E-63	-1.69E-46
MS.gene054719	MS.gene21665	0.815393	9.46E-52	-1.69E-46
MS.gene054918	MS.gene21665	0.808751	2.65E-50	-1.69E-46
MS.gene054920	MS.gene21665	0.8128	3.53E-51	-1.69E-46
MS.gene055049	MS.gene21665	0.801319	9.52E-49	-1.69E-46
MS.gene055217	MS.gene21665	0.843629	1.25E-58	-1.69E-46
MS.gene055358	MS.gene21665	0.80178	7.66E-49	-1.69E-46
MS.gene055369	MS.gene21665	0.816821	4.54E-52	-1.69E-46
MS.gene05553	MS.gene21665	0.826576	2.51E-54	-1.69E-46
MS.gene05594	MS.gene21665	0.811497	6.80E-51	-1.69E-46
MS.gene056514	MS.gene21665	0.848313	6.64E-60	-1.69E-46
MS.gene056723	MS.gene21665	0.809951	1.47E-50	-1.69E-46
MS.gene056981	MS.gene21665	0.804739	1.87E-49	-1.69E-46
MS.gene05702	MS.gene21665	0.814565	1.44E-51	-1.69E-46
MS.gene057476	MS.gene21665	0.850358	1.79E-60	-1.69E-46
MS.gene057749	MS.gene21665	0.816785	4.62E-52	-1.69E-46
MS.gene057823	MS.gene21665	0.809475	1.86E-50	-1.69E-46
MS.gene057825	MS.gene21665	0.836831	7.43E-57	-1.69E-46
MS.gene057833	MS.gene21665	0.816841	4.49E-52	-1.69E-46
MS.gene057841	MS.gene21665	0.836965	6.87E-57	-1.69E-46
MS.gene057843	MS.gene21665	0.81671	4.81E-52	-1.69E-46
MS.gene057888	MS.gene21665	0.837095	6.36E-57	-1.69E-46
MS.gene058142	MS.gene21665	0.802721	4.90E-49	-1.69E-46
MS.gene058220	MS.gene21665	0.810359	1.20E-50	-1.69E-46
MS.gene05886	MS.gene21665	0.825411	4.76E-54	-1.69E-46
MS.gene059011	MS.gene21665	0.803873	2.83E-49	-1.69E-46
MS.gene05907	MS.gene21665	0.812038	5.18E-51	-1.69E-46
MS.gene059420	MS.gene21665	0.840799	6.99E-58	-1.69E-46
MS.gene059462	MS.gene21665	0.801228	9.94E-49	-1.69E-46
MS.gene059820	MS.gene21665	0.836148	1.11E-56	-1.69E-46
MS.gene060038	MS.gene21665	0.832894	7.28E-56	-1.69E-46
MS.gene061049	MS.gene21665	0.816035	6.81E-52	-1.69E-46
MS.gene061114	MS.gene21665	-0.828869	7.07E-55	-1.69E-46
MS.gene061159	MS.gene21665	0.804492	2.11E-49	-1.69E-46
MS.gene061214	MS.gene21665	0.817093	3.94E-52	-1.69E-46
MS.gene061215	MS.gene21665	0.817406	3.35E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21665.t1	MTR_5g009290	91.216	148	7	1	1	148	1	142	5.21e-94	268
MS.gene21665.t1	MTR_8g099485	38.926	149	67	7	1	148	1	126	5.59e-19	77.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 30 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAGAACATGATGTCATTT+TGG	0.200719	5.2:-2962320	MS.gene21665:CDS
TCCTAACTGCCTTTCTTGTT+AGG	0.234019	5.2:+2962362	None:intergenic
AATTATATTGACGAATTATT+AGG	0.235132	5.2:-2962247	MS.gene21665:CDS
CTTGTCTTGAATTTCTTAAT+AGG	0.282631	5.2:-2962477	MS.gene21665:CDS
ATTGCCTACCTCATCTTTGT+AGG	0.314350	5.2:+2962402	None:intergenic
CCTAACTGCCTTTCTTGTTA+GGG	0.331209	5.2:+2962363	None:intergenic
GTTGGTTCAAGTTGATAGAA+TGG	0.448567	5.2:+2962069	None:intergenic
CCCTAACAAGAAAGGCAGTT+AGG	0.456411	5.2:-2962363	MS.gene21665:CDS
TCAAAATTTAAATGCAAAGA+AGG	0.460513	5.2:-2962154	MS.gene21665:CDS
AGAATGGTAACTCGGTCCAT+TGG	0.465675	5.2:+2962085	None:intergenic
CTAACTGCCTTTCTTGTTAG+GGG	0.467001	5.2:+2962364	None:intergenic
GAATGGTAACTCGGTCCATT+GGG	0.488126	5.2:+2962086	None:intergenic
TGAGCTTGTGGCACTCCCAA+TGG	0.488217	5.2:-2962101	MS.gene21665:CDS
AATTATTAGGAGAAAGAGAA+AGG	0.495743	5.2:-2962234	MS.gene21665:CDS
AAACAATACGGTACTGATCA+TGG	0.499897	5.2:+2962501	None:intergenic
CATATCTCCCCTAACAAGAA+AGG	0.502952	5.2:-2962371	MS.gene21665:CDS
TCAGAAGCAACATCTTTGAA+TGG	0.548770	5.2:+2962121	None:intergenic
GAAAAGGAGGAGTTGATGAA+AGG	0.556432	5.2:-2962289	MS.gene21665:CDS
TTTGGAAAAGGATGAAAAGG+AGG	0.556727	5.2:-2962302	MS.gene21665:CDS
AGGCAGTTAGGAAAAGTGTT+CGG	0.561991	5.2:-2962351	MS.gene21665:CDS
AGATGTTGCTTCTGAGCTTG+TGG	0.567179	5.2:-2962113	MS.gene21665:CDS
TTCAAGACAAGCAAACAATA+CGG	0.568501	5.2:+2962489	None:intergenic
GATGAATGTTACTAGAGTGA+AGG	0.581123	5.2:-2962212	MS.gene21665:CDS
GGCAATAACCTACAAAGATG+AGG	0.605269	5.2:-2962410	MS.gene21665:CDS
AGCAAAATTCAAAGACACAA+AGG	0.611547	5.2:-2962431	MS.gene21665:CDS
ATGAAAGGAAGAAGAAGCTG+TGG	0.613856	5.2:-2962274	MS.gene21665:CDS
AGTGAAGGTAAAGATGACAA+AGG	0.626326	5.2:-2962197	MS.gene21665:CDS
ATAACCTACAAAGATGAGGT+AGG	0.652764	5.2:-2962406	MS.gene21665:CDS
AAATTTAAATGCAAAGAAGG+AGG	0.657803	5.2:-2962151	MS.gene21665:CDS
AAGTTGATAGAATGGTAACT+CGG	0.691380	5.2:+2962077	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTATATTGACGAATTATT+AGG	-	chr5.2:2962327-2962346	MS.gene21665:CDS	15.0%
!!	TCAAAATTTAAATGCAAAGA+AGG	-	chr5.2:2962420-2962439	MS.gene21665:CDS	20.0%
!	AAATTTAAATGCAAAGAAGG+AGG	-	chr5.2:2962423-2962442	MS.gene21665:CDS	25.0%
!	AATTATTAGGAGAAAGAGAA+AGG	-	chr5.2:2962340-2962359	MS.gene21665:CDS	25.0%
!	CTTGTCTTGAATTTCTTAAT+AGG	-	chr5.2:2962097-2962116	MS.gene21665:CDS	25.0%
	AGCAAAATTCAAAGACACAA+AGG	-	chr5.2:2962143-2962162	MS.gene21665:CDS	30.0%
	TTCAAGACAAGCAAACAATA+CGG	+	chr5.2:2962088-2962107	None:intergenic	30.0%
!!	AAGTTGATAGAATGGTAACT+CGG	+	chr5.2:2962500-2962519	None:intergenic	30.0%
!!	CATTTTGGAAAAGGATGAAA+AGG	-	chr5.2:2962269-2962288	MS.gene21665:CDS	30.0%
!!	TTCAGAACATGATGTCATTT+TGG	-	chr5.2:2962254-2962273	MS.gene21665:CDS	30.0%
!!!	ACATGATGTCATTTTGGAAA+AGG	-	chr5.2:2962260-2962279	MS.gene21665:CDS	30.0%
	AGTGAAGGTAAAGATGACAA+AGG	-	chr5.2:2962377-2962396	MS.gene21665:CDS	35.0%
	ATAACCTACAAAGATGAGGT+AGG	-	chr5.2:2962168-2962187	MS.gene21665:CDS	35.0%
	GATGAATGTTACTAGAGTGA+AGG	-	chr5.2:2962362-2962381	MS.gene21665:CDS	35.0%
	TCAGAAGCAACATCTTTGAA+TGG	+	chr5.2:2962456-2962475	None:intergenic	35.0%
	TTTGGAAAAGGATGAAAAGG+AGG	-	chr5.2:2962272-2962291	MS.gene21665:CDS	35.0%
	ATGAAAGGAAGAAGAAGCTG+TGG	-	chr5.2:2962300-2962319	MS.gene21665:CDS	40.0%
	ATTGCCTACCTCATCTTTGT+AGG	+	chr5.2:2962175-2962194	None:intergenic	40.0%
	CATATCTCCCCTAACAAGAA+AGG	-	chr5.2:2962203-2962222	MS.gene21665:CDS	40.0%
	CCTAACTGCCTTTCTTGTTA+GGG	+	chr5.2:2962214-2962233	None:intergenic	40.0%
	CTAACTGCCTTTCTTGTTAG+GGG	+	chr5.2:2962213-2962232	None:intergenic	40.0%
	GGCAATAACCTACAAAGATG+AGG	-	chr5.2:2962164-2962183	MS.gene21665:CDS	40.0%
	TCCTAACTGCCTTTCTTGTT+AGG	+	chr5.2:2962215-2962234	None:intergenic	40.0%
!!	AGGCAGTTAGGAAAAGTGTT+CGG	-	chr5.2:2962223-2962242	MS.gene21665:CDS	40.0%
!!	GAAAAGGAGGAGTTGATGAA+AGG	-	chr5.2:2962285-2962304	MS.gene21665:CDS	40.0%
	AGAATGGTAACTCGGTCCAT+TGG	+	chr5.2:2962492-2962511	None:intergenic	45.0%
	CCCTAACAAGAAAGGCAGTT+AGG	-	chr5.2:2962211-2962230	MS.gene21665:CDS	45.0%
	GAATGGTAACTCGGTCCATT+GGG	+	chr5.2:2962491-2962510	None:intergenic	45.0%
!	AGATGTTGCTTCTGAGCTTG+TGG	-	chr5.2:2962461-2962480	MS.gene21665:CDS	45.0%
	TGAGCTTGTGGCACTCCCAA+TGG	-	chr5.2:2962473-2962492	MS.gene21665:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	2962075	2962521	2962075	ID=MS.gene21665
chr5.2	mRNA	2962075	2962521	2962075	ID=MS.gene21665.t1;Parent=MS.gene21665
chr5.2	exon	2962075	2962521	2962075	ID=MS.gene21665.t1.exon1;Parent=MS.gene21665.t1
chr5.2	CDS	2962075	2962521	2962075	ID=cds.MS.gene21665.t1;Parent=MS.gene21665.t1

Gene Sequence

>MS.gene21665

ATGATCAGTACCGTATTGTTTGCTTGTCTTGAATTTCTTAATAGGAAAATATCATATGAAAAATTAGCAGCAAAATTCAAAGACACAAAGGCAATAACCTACAAAGATGAGGTAGGCAATAAAAAGATCATATCTCCCCTAACAAGAAAGGCAGTTAGGAAAAGTGTTCGGTTCGCAGATTCAGAACATGATGTCATTTTGGAAAAGGATGAAAAGGAGGAGTTGATGAAAGGAAGAAGAAGCTGTGGTAGTAATTATATTGACGAATTATTAGGAGAAAGAGAAAGGATGAATGTTACTAGAGTGAAGGTAAAGATGACAAAGGAAGAAGCAGCTAAGTTACTGTCAAAATTTAAATGCAAAGAAGGAGGAGTTCTTCCATTCAAAGATGTTGCTTCTGAGCTTGTGGCACTCCCAATGGACCGAGTTACCATTCTATCAACTTGA

Protein sequence

>MS.gene21665.t1

MISTVLFACLEFLNRKISYEKLAAKFKDTKAITYKDEVGNKKIISPLTRKAVRKSVRFADSEHDVILEKDEKEELMKGRRSCGSNYIDELLGERERMNVTRVKVKMTKEEAAKLLSKFKCKEGGVLPFKDVASELVALPMDRVTILST