AlfalfaGEDB | MS.gene99857

M. sativa cultivar XinJiangDaYe / MS.gene99857

KEGG: K01904
GO: GO:0003674 GO:0003824
PFAM: PF00501.31 PF13193.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99857.t1	XP_013465640.1	94.1	560	20	3	3	559	2	551	7.90E-285	989.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99857.t1	O24146	73.3	559	132	3	1	559	1	542	3.2e-234	812.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99857.t1	G7ZYW6	94.1	560	20	3	3	559	2	551	5.7e-285	989.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene006564	MS.gene99857	PPI
MS.gene008350	MS.gene99857	PPI
MS.gene009614	MS.gene99857	PPI
MS.gene028032	MS.gene99857	PPI
MS.gene028035	MS.gene99857	PPI
MS.gene031746	MS.gene99857	PPI
MS.gene05028	MS.gene99857	PPI
MS.gene065895	MS.gene99857	PPI
MS.gene067378	MS.gene99857	PPI
MS.gene067382	MS.gene99857	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99857.t1	MTR_2g105570	94.821	560	16	4	3	559	2	551	0.0	1074
MS.gene99857.t1	MTR_4g005750	80.581	551	90	4	11	559	9	544	0.0	892
MS.gene99857.t1	MTR_5g007640	65.280	553	176	3	3	553	45	583	0.0	740
MS.gene99857.t1	MTR_4g128337	64.801	554	178	5	6	557	10	548	0.0	728
MS.gene99857.t1	MTR_4g057730	41.636	550	298	8	13	557	11	542	7.17e-144	426
MS.gene99857.t1	MTR_3g460770	38.949	552	308	8	8	553	9	537	3.71e-140	417
MS.gene99857.t1	MTR_2g095510	41.959	541	290	9	13	549	11	531	1.43e-133	400
MS.gene99857.t1	MTR_1g026310	39.741	541	308	6	13	549	26	552	2.94e-120	366
MS.gene99857.t1	MTR_4g057730	40.795	478	261	7	13	485	11	471	1.09e-118	359
MS.gene99857.t1	MTR_1g063120	39.709	549	305	12	12	549	11	544	2.32e-117	358

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene99857.t1	AT3G21240	69.009	555	154	6	6	557	15	554	786
MS.gene99857.t1	AT1G51680	68.142	565	157	6	1	557	12	561	759
MS.gene99857.t1	AT1G65060	63.071	547	184	4	10	554	31	561	701
MS.gene99857.t1	AT1G51680	67.297	529	150	6	1	521	12	525	697
MS.gene99857.t1	AT3G21240	68.465	482	135	5	6	484	15	482	678
MS.gene99857.t1	AT3G21240	68.465	482	135	5	6	484	15	482	678
MS.gene99857.t1	AT3G21230	60.695	547	203	5	11	553	26	564	662
MS.gene99857.t1	AT1G51680	67.683	492	137	5	1	484	12	489	652
MS.gene99857.t1	AT3G21230	59.125	526	199	6	11	532	26	539	614
MS.gene99857.t1	AT1G65060	61.790	458	158	3	10	465	31	473	572

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Find 122 sgRNAs with CRISPR-Local

Find 360 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAGGTTGGTTGTATAC+AGG	0.501330	2.1:-309246	MS.gene99857:CDS
AAAGATCCAGCCAGACGATG+TGG	0.660185	2.1:-313859	MS.gene99857:CDS
AAAGGAGAAGACAAATTCAG+GGG	0.673821	2.1:+314169	None:intergenic
AAATCAGGTGGTGCTCCACT+TGG	0.554196	2.1:-313471	MS.gene99857:CDS
AAATGCAGAGATGAAGATTG+TGG	0.577117	2.1:-310760	MS.gene99857:CDS
AAATTTCCAAATGCAATTCT+TGG	0.233854	2.1:-313420	MS.gene99857:CDS
AACAAATTGGGACTCCAACA+AGG	0.578787	2.1:-314221	MS.gene99857:CDS
AACAACAGGTCTACCAAAAG+GGG	0.613682	2.1:-313814	MS.gene99857:CDS
AACAGCAGCATCAGAAATTT+TGG	0.154865	2.1:+309101	None:intergenic
AAGAACTTGAAGAAACTGTT+AGG	0.460522	2.1:-313446	MS.gene99857:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGATTGATTGCAAAGGT+AGG	-	chr2.1:313357-313376	MS.gene99857:intron	30.0%
AACTACACGCTTTCAATATT+CGG	+	chr2.1:313319-313338	None:intergenic	30.0%
AAGAACTTGAAGAAACTGTT+AGG	-	chr2.1:309422-309441	MS.gene99857:intron	30.0%
AATAAGAATGAAGAGGTTGA+TGG	+	chr2.1:308511-308530	None:intergenic	30.0%
ACATAAATTAGGTGACAAGT+AGG	+	chr2.1:314211-314230	None:intergenic	30.0%
AGTATGATCTGTCTTCAATT+AGG	-	chr2.1:309368-309387	MS.gene99857:intron	30.0%
ATACATCACCTTTCATGATT+TGG	+	chr2.1:312195-312214	None:intergenic	30.0%
ATGTGCATAATGTTTGACAA+CGG	-	chr2.1:312485-312504	MS.gene99857:intron	30.0%
ATTAGGATATTGAAATCAGG+TGG	-	chr2.1:309385-309404	MS.gene99857:intron	30.0%
CATAATATATACAAGGTCTG+GGG	+	chr2.1:310249-310268	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	308434	314456	308434	ID=MS.gene99857
chr2.1	mRNA	308434	314456	308434	ID=MS.gene99857.t1;Parent=MS.gene99857
chr2.1	exon	313416	314456	313416	ID=MS.gene99857.t1.exon1;Parent=MS.gene99857.t1
chr2.1	CDS	313416	314456	313416	ID=cds.MS.gene99857.t1;Parent=MS.gene99857.t1
chr2.1	exon	310685	310883	310685	ID=MS.gene99857.t1.exon2;Parent=MS.gene99857.t1
chr2.1	CDS	310685	310883	310685	ID=cds.MS.gene99857.t1;Parent=MS.gene99857.t1
chr2.1	exon	309096	309309	309096	ID=MS.gene99857.t1.exon3;Parent=MS.gene99857.t1
chr2.1	CDS	309096	309309	309096	ID=cds.MS.gene99857.t1;Parent=MS.gene99857.t1
chr2.1	exon	308865	308970	308865	ID=MS.gene99857.t1.exon4;Parent=MS.gene99857.t1
chr2.1	CDS	308865	308970	308865	ID=cds.MS.gene99857.t1;Parent=MS.gene99857.t1
chr2.1	exon	308434	308553	308434	ID=MS.gene99857.t1.exon5;Parent=MS.gene99857.t1
chr2.1	CDS	308434	308553	308434	ID=cds.MS.gene99857.t1;Parent=MS.gene99857.t1

Gene Sequence

>MS.gene99857

ATGGCATCATCATCTAAACAAGAAACAACGGAAATAATATTCAAGTCGAAACTTCCAGACATATACATCCCCAACCATCAACCTCTTCATTCTTATTGTTTTGAAAATCTCTCCGAATTTGGTTCACGTCCATGTCTCATCAATGCCCCCACCGGAAAGGTCTACACCTACCACGACGTCCAACTCACCTCCCGTAAAGTTGCCTCTGGTCTCAACAAATTGGGACTCCAACAAGGTGAAGTCATCATGATACTCCTCCCCAATTCCCCTGAATTTGTCTTCTCCTTTCTTGGCGCTTCTTATCTCGGCGCCATAGCCACCGCAGCCAATCCTTTCTTCACGGCTGCTGAGATTGGAAAGCAAGCAAAAGCCTCCAACAGCAAGTTGATCATAACACAACAATGTTACTACGACAAAGTCAAAGATTTGTTGAACAACAATGATCATCATAAGGTGGTGGACGTGGTGTTGGTAGACTCTTCTTCTTCTTCTTCTTCTGCCATAGATGATAATAACAATGATCATGTGCATTTCTCAACGCTGATCGAAGCAGATGAGAATGATCTTCCTGATGCAAAGATCCAGCCAGACGATGTGGTTGCGCTGCCATATTCATCAGGAACAACAGGTCTACCAAAAGGGGTGATGTTAACACACAAAGGTTTGGTGAGTAGCATAGCACAACAAGTAGATGGAGAGAATCCAAATCTATATTACAGCAGTGAAGATGTGATACTTTGTGTCCTTCCTCTGTTTCATATTTATTCACTAAACTCTGTTTTGCTCTGCGGACTCAGAGCAAAAGCTACCATTCTCTTGATGCCAAAATTCGACATAAATGCATTCTTGAATCTTGTTAACAAACATGGAGTAACAGTTGCACCGGTTGTTCCTCCGATTGTATTGGCAATAGCAAAATCACCAGATCTTAATAAGTATGATCTGTCTTCAATTAGGATATTGAAATCAGGTGGTGCTCCACTTGGTAAAGAACTTGAAGAAACTGTTAGGACTAAATTTCCAAATGCAATTCTTGGACAGGTAACTACTACCACCTAATTCATTCATTTCTTTCTTGTTTTTACATTTCTTATTCATTCCCTCACGCCACGCATTTTAATTCTTTTTCCTGCACTTATACGTTAGGGGATATTTTATTACTTTTCCATACTATACTACAAATTAAAACGAAATTGTTGCACCGAGATATCAGATTTTCATGATTATGACACAATCAATGAAAATGAGTAGCTAGCTCTCCATAGATTGCCTTTCTTCATTCATGTTAAAATTTGCATTTTCCTTTAAATTATTCACTGTGATATAATTTTACTGTATTTTTTAGAATTAAATAAATGACAACATAGTATTAGTATATATGACAGTAAATATTTTACGTTTTCTTACATCAATATCATATAGGATATTCTCATTCTTAATATCAATAAATTTACTCATATGACCAGTTGTTTGTTGTTTTTTATGGTTACATGTCAATGCCAACGCCTGGTTACATCTTTATGTTTCACATCAGTTTTGACCTCAAAAATCATGTCTACCTAAATTATATTGTGTGTGTCTTTAATATGAAACTGATAGCTCACCAACGAAATAATATATGAAACTAATATAAGTTCTTATGCATATTTTTTCCTAGATTTCTGTATGATTATTTAGTACTAGTTTGTGATCATGCTCGTGTATAATATTTTCGATTTAGATCATCTAAAATGAATATTGTGTAAAATGAGAAAAATCAAAATGATTGTTAATTTTACAAACTTGTTATTTTTTTTTTGTCCGGAATTCGAACCCCAGACCTTGTATATATTATGTATTGTCCTTACCAACTAAGCTAAACTCACGGGGACAACAAACTTGTTATTTGTTATGACGTAGGAATATCTAAAATAACAAACTTGTTATTTGTTATGACGTAGGAATATCTAAAATATTTACCGATAATGTGTGTTATACGAAAACTTCTATGGTCCTGACCTCTATGGTTCTGATTTAAAAAAAAAAAAAAAACATGTTTCAAAGCATAGTTTCAATGATTATTTTTATTTTTTTCATTTTTTATCGGGAAACAACAACTATTTTGTAGTTCTTTTTATAATTTTTTTGAAAGGATCGACTTTTTAGTTTTTAGTACTTGAAATCACTTCTAAATATATTTTTGAGTTTATTGTGAATATTGTAACTGAATATATATAAATTATGTAGAAAATGCTGAAATTGACTAAAAAAAAATTATTTCATATCAAGATAATTTTATTTAATGGGAAATGCTAATGAATGTCTTTAGGGCATTCTTTAAAGGTTTAAAATAGTAAGTTAAGACTATCTTCAATGGTGAGGCCTTAACCATTTAATACCCGATATCTAATAGGTGCCTACCCAACAATCTTAAGTGGGACTCACACCATTTTAATACTCCCTCCGATCCTTTTTATAAGAAGAAAAACCAAAAATCATCAAAACCAAGACAATCATTATTTGTTATACTTTTTTGAATGTTTTTACTAATATACCCTCATAAAAATTTGTGAAGTATTAAATAGACCCACTAATATTGAAAAGACCAACATTAAATGAGGGCTAGAAAAGTTTTTTAAACAATAAATGTACCTTAAAAGTTGTGACCTTTTCTTATAAATAGTACCAAGATTTTTTTTGAAAATTTTCTTATAAATAGGACTGGAGGGAGTATTAAAAAATTTATATGTACTAATATAAAGTTATTGATAGTTGATTTGTGCGTCCCATTATATTTAGGAAATTTGTGTGAGATGTTGTTTTGGACGGGTTGAATGCTTATTAAGTACTACTATTAAAAAATTATAAATGAGTGATGTGTACACGTGAGATCCATTTAAGTAACAAAAAATGAGGTCTCCATTGTGGATGCTTTAATAATCTTAAAACTTTGGAACTTGTGTATTCAATGTCTTGAAAATTGAACTGTTTGACTTTTTAGTTAATAGTAATTTATAATTGGGAATCCTGAATAGTGCCTTTAGAGCATCTACAATGGTGAGGTCTTCACCATTTAAGACCTGGTACCTAATAGGTACCCCCATTGTGGGAAAAAAAAGTGAGTGTCTTAAAAGATGCAAGGTCTAAATTAAGACCTCATTCTTAAATGGGCCCACATCACTTTTTTATTAAAATAATAATTAAAAAGTGTATTAAAATAGATAGTGTATTAAATAAATAATATTGGTGGGACCCAATTAGACCTTTTGTTAGAGGATCTCCATTGGAGTGGTTGAGTACCTATTAGGTATTATTATTAAAAAATTATTAAAGAGTGATGTGTCAATGTGGGACCCATTTAAGACCTTAAAGATGAGAGTCTCCATTGTGGATGCTCTTAGGGCACTCATTAACAAGACATTTATTCAATACTTCATCCGGTCACTATTATAAAAAAAAATAAATTAAAATATACATGATTAAATTTAATGTACACATTTCAATACATTATTTTACATTTGAATTATTAAATTGTGTCTTAAGGCACAAAGTTAGCATTTTCCTCGTTTAAATATAATAAATGGAACATTACAGGGATACGGAATGACTGAGGCGGGGCCAGTGTTAACGATGAGTTTAGCATTTGCTAAAGAACCAGTGAATGTAAAAGCAGGTGCATGTGGAACTGTTGTAAGAAATGCAGAGATGAAGATTGTGGATCCTGACACTGGTAAATCTTTACCTCGAAACCAATCTGGTGAAATCTGCATAAGAGGCGACCAAATCATGAAAGGTGATGTATATATATCATTCTATTAACATTATTCATCCACTTTCCAAATTTAATCATTTCCTTCTTTTAAATTAATTTAATATACTTTGGGCTTTATTATAATCTTGTTTTAATCTCGTTTTTAATAATAGGAAAAGGGGTGCTATCACAGAATGCACTGTTAATCACCTAATATACTTTACTTAATTAAAATTTATCTCAATTTATCATTTTTTACACAATCACATTTCATCATATATAGAATATAAATATAAATTACTTAATAACTTTTTTGAAAAAATGTGCATAATGTTTGACAACGGTATATATGAGAATGAGGTACAAAAAGTACCTTTTAAATTATCTCATCCAAATTGGCACCACAAGAAATCGAACCTGCGATTTCGAGAGGTCTCAAACCAATATCATCAGGTCAACCCAAGTGGATTTAAAGTACATTTTACTTAATAGCTAAATCATTCCTTTAGAACGAGTAAAAATTGACTAAATTAGGAAGAAAGATAGCACTCAAAACTACCCATTTCGCCTCTTTTATAAACACCTTTTGAAGAAAAACAAACTATATAAAATGGATTTTTTTTTATAAAATTAGTTCAATTTTTTAAAAAATCCTGTAAAAAAAATCTATTTTTTGAGAAGCTGCTCTTAACAGCTTCTCATTTGAAGTTGTTTTTAGATTCTGATTTTTTTTTTAAAAAAAAATTGTAACAAACATATGCAAAACTCTTAAAAGTGATTATTTTTTTTAAAATAAGTGATTTTTTCTTAAAAAAGCTATAACAAACGGACTCTAAGTCCCAAAGGTATAAATAAGGCTTTCGCTGACGGATACCTGTATTAATTATATTTTACAACTGCTCTGGAGAGTTTGAACTCAACACATTGATATTATTAGACAAAGCTCTTACCAAGCTAGCACTTTGAAATATTGGAAACAAAGGTTCTTTTTAGAGGCGTGAGAAATCAAGGTTAGCCAATTTGGATAAATTAGGCACATTAAATAAGCTATCTATTTTATATATGTTAACACATTTAAAAGTGTTTACCAATTAAATTGTTATTATAAATTTTTATACTAGTTAACATGACACAAGAAGTATTTAAGGGTTGCCGAATATTGAAAGCGTGTAGTTAGATTAGAGCGCAACATGAAATGATTGATTGCAAAGGTAGGTTGAAGTGTCCTCTCTTCATGGGATGTTGTTGACTCCAAACAAAACAATCCAGCTCCACTTTCATGTAATGTAATAACCAAAAAAAATGTATGTAGACACATACATACCAATCTTTTTTTTAATAAATTAATTATGAAAAATTTGTATTAACTATATATGAACAAGTGAAAATTGATTAATTAAATAATGAAATGATGCAGGTTATCTAAATGATCGAGAGGCAACAGAGAGAACCATAGACAAAGAAGGTTGGTTGTATACAGGTGATATTGGGTATATTGATGAAGATGATGAGTTATTCATTGTTGATAGATTGAAGGAATTAATCAAATACAAAGGATTTCAAGTTGCTCCAGCTGAACTTGAAGCTCTTCTTCTCTCTCATCCCAAAATTTCTGATGCTGCTGTTGTCCCGTAAGTCTAGTCTTTTCTTATGCTATGAAATTTCCATTCATATTATTAATTCAATTCAATTCAATTCAATTCAATGTTTATTTATTTACCATTCATGCATGCATATGCATAAATTTAAATTTTAGAATGAAGGATGAAGCGGCTGGAGAGGTACCTGTTGCATTTGTGGTGAGATCAAATGGTTACACTGACTTAACTCAAGATGAAATTAAGCACTTTATCTCCAAACAGGTTCTTTTTCTTCCTTTTAACTTTTACATGAAAATACATAATTTTTGGGAGGTAAGGTCTCGTTGAATAAACTTAAATTGCATAATGAATTGACTAACAAAACAAAAAGTTAGTAGGAAAAATTGATTAAATATAAGGACATGAGCTTGTGAGTTAAGATCTTAGAGCGGGTGCATTAATTACCTACTTGTCACCTAATTTATGTATGTACTTCTCACCTAAATGTTGTGTGTGAGGTATAGCAACTTTCTTACACTACAAGTTTAGCAAATTAATGTTAATAATTGAGGTGTTTGGGTTTGATATTACAGGTGGTGTTTTACAAAAGAATAAATCAAGTATTCTTCATTGATGCAATTCCCAAGTCACCATCAGGCAAAATCTTGCGCAAGGATTTGAGAGCAAAGCTAGCAGCAGGTGTTCCACATTGA

Protein sequence

>MS.gene99857.t1

MASSSKQETTEIIFKSKLPDIYIPNHQPLHSYCFENLSEFGSRPCLINAPTGKVYTYHDVQLTSRKVASGLNKLGLQQGEVIMILLPNSPEFVFSFLGASYLGAIATAANPFFTAAEIGKQAKASNSKLIITQQCYYDKVKDLLNNNDHHKVVDVVLVDSSSSSSSAIDDNNNDHVHFSTLIEADENDLPDAKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVSSIAQQVDGENPNLYYSSEDVILCVLPLFHIYSLNSVLLCGLRAKATILLMPKFDINAFLNLVNKHGVTVAPVVPPIVLAIAKSPDLNKYDLSSIRILKSGGAPLGKELEETVRTKFPNAILGQGYGMTEAGPVLTMSLAFAKEPVNVKAGACGTVVRNAEMKIVDPDTGKSLPRNQSGEICIRGDQIMKGYLNDREATERTIDKEGWLYTGDIGYIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLSHPKISDAAVVPMKDEAAGEVPVAFVVRSNGYTDLTQDEIKHFISKQVVFYKRINQVFFIDAIPKSPSGKILRKDLRAKLAAGVPH