AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22218


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22218.t1 MTR_3g449950 97.106 311 9 0 1 311 1 311 0.0 618
MS.gene22218.t1 MTR_3g449990 66.436 289 88 3 1 281 1 288 1.39e-114 343
MS.gene22218.t1 MTR_5g090420 58.131 289 106 5 1 281 1 282 5.94e-92 284
MS.gene22218.t1 MTR_1g069345 32.212 208 132 3 46 252 7 206 3.36e-24 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22218.t1 AT5G08560 44.781 297 147 6 1 283 1 294 2.21e-71 231
MS.gene22218.t1 AT5G08560 44.781 297 147 6 1 283 1 294 2.21e-71 231
MS.gene22218.t1 AT5G08560 44.781 297 147 6 1 283 1 294 2.21e-71 231
MS.gene22218.t1 AT5G43920 33.333 240 152 5 46 281 7 242 3.24e-27 111

Find 70 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGCCAAGGTGGTTCTTC+CGG 0.265995 3.4:+40153439 MS.gene22218:CDS
CTGGCCAAGGTGGTTCTTCC+GGG 0.287686 3.4:+40153440 MS.gene22218:CDS
AACTTGTCCAGAGCTGATTT+TGG 0.291966 3.4:+40153718 MS.gene22218:CDS
GAGGAGGAGGGGTTCGAAAT+AGG 0.313413 3.4:-40153825 None:intergenic
ACCTAATAGTTCATCTTCTA+TGG 0.313662 3.4:+40152967 MS.gene22218:CDS
GAAGTATAAGCTTGATTAGT+AGG 0.346896 3.4:-40153667 None:intergenic
GTCGTCCCCTTCGGCTGATC+GGG 0.347053 3.4:-40153029 None:intergenic
CTGTTTATGCAGCAAGTACT+TGG 0.358690 3.4:+40153196 MS.gene22218:CDS
AGCAAGTACTTGGCGGTGAT+TGG 0.361938 3.4:+40153206 MS.gene22218:CDS
CTAGCCACATTGAAACAAAT+TGG 0.364435 3.4:+40153238 MS.gene22218:CDS
TCTTCTATGGAGCACGTTGT+AGG 0.395510 3.4:+40152980 MS.gene22218:CDS
CGTCGTCCCCTTCGGCTGAT+CGG 0.398819 3.4:-40153030 None:intergenic
GACGAAGTTGTTGGCACAAA+AGG 0.398912 3.4:+40153049 MS.gene22218:CDS
TGAGCTTATTGATGGGGATA+AGG 0.407133 3.4:+40153321 MS.gene22218:CDS
TCCATAGAAGATGAACTATT+AGG 0.439662 3.4:-40152968 None:intergenic
TTTAAAATCTATTGAGGAGG+AGG 0.444056 3.4:-40153838 None:intergenic
TCCTGCTTCATGTCACCCTC+TGG 0.448042 3.4:+40153421 MS.gene22218:CDS
AAATCTTCCAAAATCAGCTC+TGG 0.468825 3.4:-40153725 None:intergenic
AGGCTCAACAAGTGACTTGA+TGG 0.473288 3.4:+40153000 MS.gene22218:CDS
ACCAAGCGAATACATCGCTT+TGG 0.483200 3.4:-40153107 None:intergenic
TTAAAATCTATTGAGGAGGA+GGG 0.486898 3.4:-40153837 None:intergenic
TTCATGTCACCCTCTGGCCA+AGG 0.491036 3.4:+40153427 MS.gene22218:CDS
AACAACTTCGTCGTCCCCTT+CGG 0.491520 3.4:-40153038 None:intergenic
ACATAATTTAAAATCTATTG+AGG 0.496725 3.4:-40153844 None:intergenic
GCAAGTACTTGGCGGTGATT+GGG 0.500806 3.4:+40153207 MS.gene22218:CDS
AGAAGATGAAAGCATCGTTA+AGG 0.501049 3.4:+40153264 MS.gene22218:CDS
GAACACCTAGAGGAGGAGTC+TGG 0.502621 3.4:+40153145 MS.gene22218:CDS
TTCCTTGTGTCGCAACGCGT+TGG 0.514667 3.4:+40153597 MS.gene22218:CDS
ATTTGTCAGTACTGCTGAAA+GGG 0.515762 3.4:+40153744 MS.gene22218:CDS
TGTTTCTGATGATAACGAAT+CGG 0.517284 3.4:+40153693 MS.gene22218:CDS
GCCAGAGGGTGACATGAAGC+AGG 0.519258 3.4:-40153422 None:intergenic
ATCCAACGCGTTGCGACACA+AGG 0.527126 3.4:-40153599 None:intergenic
TGCCCTTACAAAATCTCGCC+CGG 0.534345 3.4:-40153458 None:intergenic
ACCAAAGCGATGTATTCGCT+TGG 0.535080 3.4:+40153106 MS.gene22218:CDS
TCGTCATGTTCAGATGACTT+TGG 0.542727 3.4:+40153631 MS.gene22218:CDS
GAAGGGGACGACGAAGTTGT+TGG 0.544222 3.4:+40153040 MS.gene22218:CDS
ACGAAGTTGTTGGCACAAAA+GGG 0.550371 3.4:+40153050 MS.gene22218:CDS
TCAGGTATCATTACAGTCGA+GGG 0.553135 3.4:-40153523 None:intergenic
GTATGCCAGACTCCTCCTCT+AGG 0.556041 3.4:-40153150 None:intergenic
CAAGGAAGCTACTCGTTCCA+TGG 0.556398 3.4:-40153581 None:intergenic
AGCTACTCGTTCCATGGTGA+GGG 0.556455 3.4:-40153575 None:intergenic
TTCAGGTATCATTACAGTCG+AGG 0.557702 3.4:-40153524 None:intergenic
TTTATGCAGCAAGTACTTGG+CGG 0.558318 3.4:+40153199 MS.gene22218:CDS
GCTCCGCCCCGATCAGCCGA+AGG 0.562506 3.4:+40153022 MS.gene22218:CDS
GTTGGTTATTATTTCATGGG+AGG 0.563350 3.4:+40152902 None:intergenic
TAAAATCTATTGAGGAGGAG+GGG 0.564663 3.4:-40153836 None:intergenic
TTATTATTTCATGGGAGGTG+TGG 0.569082 3.4:+40152907 None:intergenic
CTGCTCCAATATTAAAAGTG+AGG 0.572912 3.4:-40153290 None:intergenic
GAAGAACCACCTTGGCCAGA+GGG 0.573757 3.4:-40153436 None:intergenic
CTCCGCCCCGATCAGCCGAA+GGG 0.575025 3.4:+40153023 MS.gene22218:CDS
AAGCTACTCGTTCCATGGTG+AGG 0.576410 3.4:-40153576 None:intergenic
GATTTGTCAGTACTGCTGAA+AGG 0.578338 3.4:+40153743 MS.gene22218:CDS
ATGTCACCCTCTGGCCAAGG+TGG 0.595449 3.4:+40153430 MS.gene22218:CDS
TCCGCCCCGATCAGCCGAAG+GGG 0.596037 3.4:+40153024 MS.gene22218:CDS
TATACCAATTTGTTTCAATG+TGG 0.605452 3.4:-40153242 None:intergenic
CTCGCCCGGAAGAACCACCT+TGG 0.608997 3.4:-40153444 None:intergenic
TCGTCCCCTTCGGCTGATCG+GGG 0.610635 3.4:-40153028 None:intergenic
TAATTTAAAATCTATTGAGG+AGG 0.624087 3.4:-40153841 None:intergenic
TTTACCGCAGACGAATTCAA+AGG 0.627122 3.4:-40153172 None:intergenic
GGAAGAACCACCTTGGCCAG+AGG 0.628654 3.4:-40153437 None:intergenic
TCCCCTTCGGCTGATCGGGG+CGG 0.629349 3.4:-40153025 None:intergenic
TGTCAGTACTGCTGAAAGGG+TGG 0.632691 3.4:+40153747 MS.gene22218:CDS
TCGAGGCATTGAATACATTG+AGG 0.634411 3.4:+40153347 MS.gene22218:CDS
CAGGTATCATTACAGTCGAG+GGG 0.643753 3.4:-40153522 None:intergenic
GAGTGGGGAACACCTAGAGG+AGG 0.656225 3.4:+40153138 MS.gene22218:CDS
CATACCTTTGAATTCGTCTG+CGG 0.656627 3.4:+40153168 MS.gene22218:CDS
AAATCGGTTGAAACTGCTGG+AGG 0.679706 3.4:+40153483 MS.gene22218:CDS
AAAGAGTGGGGAACACCTAG+AGG 0.683816 3.4:+40153135 MS.gene22218:CDS
AGTTGAACAAGCCCTCACCA+TGG 0.691356 3.4:+40153564 MS.gene22218:CDS
TGATGGGGATAAGGTCATCG+AGG 0.725311 3.4:+40153330 MS.gene22218:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTTGAGCTTATTGA+TGG + chr3.4:40153313-40153332 MS.gene22218:CDS 20.0%
!!! AATTTTTTGAGCTTATTGAT+GGG + chr3.4:40153314-40153333 MS.gene22218:CDS 20.0%
!! ATTTTGTAAGGGCAAAAAAT+CGG + chr3.4:40153467-40153486 MS.gene22218:CDS 25.0%
!! TATACCAATTTGTTTCAATG+TGG - chr3.4:40153245-40153264 None:intergenic 25.0%
!!! ATTTTTTGAGCTTATTGATG+GGG + chr3.4:40153315-40153334 MS.gene22218:CDS 25.0%
AAAAGGGAGCTTAAAAAGAA+GGG + chr3.4:40153066-40153085 MS.gene22218:CDS 30.0%
ACCTAATAGTTCATCTTCTA+TGG + chr3.4:40152967-40152986 MS.gene22218:CDS 30.0%
GAAGTATAAGCTTGATTAGT+AGG - chr3.4:40153670-40153689 None:intergenic 30.0%
TAAAAAGAAGGGAGTTTGTA+AGG + chr3.4:40153077-40153096 MS.gene22218:CDS 30.0%
TCCATAGAAGATGAACTATT+AGG - chr3.4:40152971-40152990 None:intergenic 30.0%
! AATTTCGTTCTTTTCAAAGC+TGG - chr3.4:40152944-40152963 None:intergenic 30.0%
! AGATGTTCTAATCTCTTTTC+AGG - chr3.4:40153544-40153563 None:intergenic 30.0%
! TGTTTCTGATGATAACGAAT+CGG + chr3.4:40153693-40153712 MS.gene22218:CDS 30.0%
AAAAAATCGGTTGAAACTGC+TGG + chr3.4:40153480-40153499 MS.gene22218:CDS 35.0%
AAATCTTCCAAAATCAGCTC+TGG - chr3.4:40153728-40153747 None:intergenic 35.0%
ATTTGTCAGTACTGCTGAAA+GGG + chr3.4:40153744-40153763 MS.gene22218:CDS 35.0%
CAAAAGGGAGCTTAAAAAGA+AGG + chr3.4:40153065-40153084 MS.gene22218:CDS 35.0%
CTAGCCACATTGAAACAAAT+TGG + chr3.4:40153238-40153257 MS.gene22218:CDS 35.0%
CTGCTCCAATATTAAAAGTG+AGG - chr3.4:40153293-40153312 None:intergenic 35.0%
!! AGAAGATGAAAGCATCGTTA+AGG + chr3.4:40153264-40153283 MS.gene22218:CDS 35.0%
ACGAAGTTGTTGGCACAAAA+GGG + chr3.4:40153050-40153069 MS.gene22218:CDS 40.0%
CATACCTTTGAATTCGTCTG+CGG + chr3.4:40153168-40153187 MS.gene22218:CDS 40.0%
CGCTTGGTTACAAAAAGAGT+GGG + chr3.4:40153122-40153141 MS.gene22218:CDS 40.0%
CTGTTTATGCAGCAAGTACT+TGG + chr3.4:40153196-40153215 MS.gene22218:CDS 40.0%
GATTTGTCAGTACTGCTGAA+AGG + chr3.4:40153743-40153762 MS.gene22218:CDS 40.0%
GCTTGGTTACAAAAAGAGTG+GGG + chr3.4:40153123-40153142 MS.gene22218:CDS 40.0%
TCAGGTATCATTACAGTCGA+GGG - chr3.4:40153526-40153545 None:intergenic 40.0%
TCGCTTGGTTACAAAAAGAG+TGG + chr3.4:40153121-40153140 MS.gene22218:CDS 40.0%
TCGTCATGTTCAGATGACTT+TGG + chr3.4:40153631-40153650 MS.gene22218:CDS 40.0%
TGAGCTTATTGATGGGGATA+AGG + chr3.4:40153321-40153340 MS.gene22218:CDS 40.0%
TTCAGGTATCATTACAGTCG+AGG - chr3.4:40153527-40153546 None:intergenic 40.0%
TTTACCGCAGACGAATTCAA+AGG - chr3.4:40153175-40153194 None:intergenic 40.0%
TTTATGCAGCAAGTACTTGG+CGG + chr3.4:40153199-40153218 MS.gene22218:CDS 40.0%
!! AACTTGTCCAGAGCTGATTT+TGG + chr3.4:40153718-40153737 MS.gene22218:CDS 40.0%
!! GGCTGCCTCACTTTTAATAT+TGG + chr3.4:40153285-40153304 MS.gene22218:CDS 40.0%
!! TCGAGGCATTGAATACATTG+AGG + chr3.4:40153347-40153366 MS.gene22218:CDS 40.0%
AAATCGGTTGAAACTGCTGG+AGG + chr3.4:40153483-40153502 MS.gene22218:CDS 45.0%
ACCAAAGCGATGTATTCGCT+TGG + chr3.4:40153106-40153125 MS.gene22218:CDS 45.0%
ACCAAGCGAATACATCGCTT+TGG - chr3.4:40153110-40153129 None:intergenic 45.0%
AGGCTCAACAAGTGACTTGA+TGG + chr3.4:40153000-40153019 MS.gene22218:CDS 45.0%
CAGGTATCATTACAGTCGAG+GGG - chr3.4:40153525-40153544 None:intergenic 45.0%
GACGAAGTTGTTGGCACAAA+AGG + chr3.4:40153049-40153068 MS.gene22218:CDS 45.0%
TCTTCTATGGAGCACGTTGT+AGG + chr3.4:40152980-40152999 MS.gene22218:CDS 45.0%
! TTCCGGGCGAGATTTTGTAA+GGG + chr3.4:40153456-40153475 MS.gene22218:CDS 45.0%
AAAGAGTGGGGAACACCTAG+AGG + chr3.4:40153135-40153154 MS.gene22218:CDS 50.0%
AACAACTTCGTCGTCCCCTT+CGG - chr3.4:40153041-40153060 None:intergenic 50.0%
AAGCTACTCGTTCCATGGTG+AGG - chr3.4:40153579-40153598 None:intergenic 50.0%
AGCAAGTACTTGGCGGTGAT+TGG + chr3.4:40153206-40153225 MS.gene22218:CDS 50.0%
AGCTACTCGTTCCATGGTGA+GGG - chr3.4:40153578-40153597 None:intergenic 50.0%
AGTTGAACAAGCCCTCACCA+TGG + chr3.4:40153564-40153583 MS.gene22218:CDS 50.0%
CAAGGAAGCTACTCGTTCCA+TGG - chr3.4:40153584-40153603 None:intergenic 50.0%
GCAAGTACTTGGCGGTGATT+GGG + chr3.4:40153207-40153226 MS.gene22218:CDS 50.0%
TGATGGGGATAAGGTCATCG+AGG + chr3.4:40153330-40153349 MS.gene22218:CDS 50.0%
TGCCCTTACAAAATCTCGCC+CGG - chr3.4:40153461-40153480 None:intergenic 50.0%
TGTCAGTACTGCTGAAAGGG+TGG + chr3.4:40153747-40153766 MS.gene22218:CDS 50.0%
! CTTCCGGGCGAGATTTTGTA+AGG + chr3.4:40153455-40153474 MS.gene22218:CDS 50.0%
ATCCAACGCGTTGCGACACA+AGG - chr3.4:40153602-40153621 None:intergenic 55.0%
GAACACCTAGAGGAGGAGTC+TGG + chr3.4:40153145-40153164 MS.gene22218:CDS 55.0%
GAAGAACCACCTTGGCCAGA+GGG - chr3.4:40153439-40153458 None:intergenic 55.0%
GAAGGGGACGACGAAGTTGT+TGG + chr3.4:40153040-40153059 MS.gene22218:CDS 55.0%
GAGGAGGAGGGGTTCGAAAT+AGG - chr3.4:40153828-40153847 None:intergenic 55.0%
GTATGCCAGACTCCTCCTCT+AGG - chr3.4:40153153-40153172 None:intergenic 55.0%
TCCTGCTTCATGTCACCCTC+TGG + chr3.4:40153421-40153440 MS.gene22218:CDS 55.0%
TCTGGCCAAGGTGGTTCTTC+CGG + chr3.4:40153439-40153458 MS.gene22218:CDS 55.0%
TTCATGTCACCCTCTGGCCA+AGG + chr3.4:40153427-40153446 MS.gene22218:CDS 55.0%
TTCCTTGTGTCGCAACGCGT+TGG + chr3.4:40153597-40153616 MS.gene22218:CDS 55.0%
ATGTCACCCTCTGGCCAAGG+TGG + chr3.4:40153430-40153449 MS.gene22218:CDS 60.0%
CTGGCCAAGGTGGTTCTTCC+GGG + chr3.4:40153440-40153459 MS.gene22218:CDS 60.0%
GAGTGGGGAACACCTAGAGG+AGG + chr3.4:40153138-40153157 MS.gene22218:CDS 60.0%
GCCAGAGGGTGACATGAAGC+AGG - chr3.4:40153425-40153444 None:intergenic 60.0%
GGAAGAACCACCTTGGCCAG+AGG - chr3.4:40153440-40153459 None:intergenic 60.0%
CGTCGTCCCCTTCGGCTGAT+CGG - chr3.4:40153033-40153052 None:intergenic 65.0%
CTCGCCCGGAAGAACCACCT+TGG - chr3.4:40153447-40153466 None:intergenic 65.0%
GTCGTCCCCTTCGGCTGATC+GGG - chr3.4:40153032-40153051 None:intergenic 65.0%
TCGTCCCCTTCGGCTGATCG+GGG - chr3.4:40153031-40153050 None:intergenic 65.0%
CTCCGCCCCGATCAGCCGAA+GGG + chr3.4:40153023-40153042 MS.gene22218:CDS 70.0%
TCCCCTTCGGCTGATCGGGG+CGG - chr3.4:40153028-40153047 None:intergenic 70.0%
TCCGCCCCGATCAGCCGAAG+GGG + chr3.4:40153024-40153043 MS.gene22218:CDS 70.0%
GCTCCGCCCCGATCAGCCGA+AGG + chr3.4:40153022-40153041 MS.gene22218:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 40152917 40153852 40152917 ID=MS.gene22218
chr3.4 mRNA 40152917 40153852 40152917 ID=MS.gene22218.t1;Parent=MS.gene22218
chr3.4 exon 40152917 40153852 40152917 ID=MS.gene22218.t1.exon1;Parent=MS.gene22218.t1
chr3.4 CDS 40152917 40153852 40152917 ID=cds.MS.gene22218.t1;Parent=MS.gene22218.t1
Gene Sequence

>MS.gene22218

ATGGGAGGTGTGGAAGATGAAGAACCAGCTTTGAAAAGAACGAAATTGTCACCTAATAGTTCATCTTCTATGGAGCACGTTGTAGGCTCAACAAGTGACTTGATGGCTCCGCCCCGATCAGCCGAAGGGGACGACGAAGTTGTTGGCACAAAAGGGAGCTTAAAAAGAAGGGAGTTTGTAAGGATGATCACCAAAGCGATGTATTCGCTTGGTTACAAAAAGAGTGGGGAACACCTAGAGGAGGAGTCTGGCATACCTTTGAATTCGTCTGCGGTAAATCTGTTTATGCAGCAAGTACTTGGCGGTGATTGGGATGAAAGTCTAGCCACATTGAAACAAATTGGTATAGAAGATGAAAGCATCGTTAAGGCTGCCTCACTTTTAATATTGGAGCAGAAATTTTTTGAGCTTATTGATGGGGATAAGGTCATCGAGGCATTGAATACATTGAGGAATCAGATAACACAACTTTGTGTTGATAGTACTAGAATTCGTGAACTTTCGTCCTGCTTCATGTCACCCTCTGGCCAAGGTGGTTCTTCCGGGCGAGATTTTGTAAGGGCAAAAAATCGGTTGAAACTGCTGGAGGAGCTACAAAAACTGTTCCCCTCGACTGTAATGATACCTGAAAAGAGATTAGAACATCTAGTTGAACAAGCCCTCACCATGGAACGAGTAGCTTCCTTGTGTCGCAACGCGTTGGATAAGAAGATGTCGTCATGTTCAGATGACTTTGGACAAACTCAGATACCTACTAATCAAGCTTATACTTCTGTTGTTTCTGATGATAACGAATCGGTTAACTTGTCCAGAGCTGATTTTGGAAGATTTGTCAGTACTGCTGAAAGGGTGGACAAGCTAGAAGAAAAATACAAAATGTTATTGAGCATGCTTTCTGAACTCATAAGCCTATTTCGAACCCCTCCTCCTCAATAG

Protein sequence

>MS.gene22218.t1

MGGVEDEEPALKRTKLSPNSSSSMEHVVGSTSDLMAPPRSAEGDDEVVGTKGSLKRREFVRMITKAMYSLGYKKSGEHLEEESGIPLNSSAVNLFMQQVLGGDWDESLATLKQIGIEDESIVKAASLLILEQKFFELIDGDKVIEALNTLRNQITQLCVDSTRIRELSSCFMSPSGQGGSSGRDFVRAKNRLKLLEELQKLFPSTVMIPEKRLEHLVEQALTMERVASLCRNALDKKMSSCSDDFGQTQIPTNQAYTSVVSDDNESVNLSRADFGRFVSTAERVDKLEEKYKMLLSMLSELISLFRTPPPQ