AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24483


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24483.t1 MTR_1g017950 99.487 195 1 0 1 195 1 195 4.05e-146 404
MS.gene24483.t1 MTR_4g106800 66.857 175 57 1 2 176 3 176 1.70e-87 255
MS.gene24483.t1 MTR_3g070050 58.757 177 69 2 1 175 1 175 3.02e-70 212
MS.gene24483.t1 MTR_8g031490 50.820 183 83 3 1 182 1 177 2.64e-61 189
MS.gene24483.t1 MTR_8g097300 54.802 177 68 4 1 175 1 167 9.79e-61 188
MS.gene24483.t1 MTR_7g076720 50.857 175 82 2 1 175 1 171 3.28e-60 187
MS.gene24483.t1 MTR_3g070050 53.731 134 58 2 44 175 2 133 3.89e-45 146
MS.gene24483.t1 MTR_3g070050 47.826 69 36 0 3 71 61 129 1.42e-18 78.6
MS.gene24483.t1 MTR_1g106100 58.559 111 40 2 59 168 20 125 6.39e-38 128
MS.gene24483.t1 MTR_3g070033 66.038 53 18 0 123 175 6 58 3.58e-18 75.5
MS.gene24483.t1 MTR_3g070033 46.939 49 26 0 23 71 6 54 6.90e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24483.t1 AT1G10200 85.246 183 27 0 1 183 1 183 2.62e-116 328
MS.gene24483.t1 AT1G10200 82.014 139 25 0 45 183 3 141 2.77e-82 241
MS.gene24483.t1 AT1G10200 44.444 72 40 0 3 74 61 132 2.24e-16 72.8
MS.gene24483.t1 AT2G39900 58.192 177 69 2 1 175 1 174 2.13e-69 210
MS.gene24483.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene24483.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene24483.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene24483.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene24483.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene24483.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene24483.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene24483.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene24483.t1 AT2G45800 50.000 186 86 2 1 183 1 182 3.75e-61 190
MS.gene24483.t1 AT1G01780 48.649 185 87 3 1 183 1 179 2.17e-60 187
MS.gene24483.t1 AT3G61230 47.917 192 93 3 2 190 3 190 8.55e-60 186
MS.gene24483.t1 AT3G61230 36.792 106 67 0 3 108 100 205 3.58e-17 76.6
MS.gene24483.t1 AT3G55770 46.479 213 70 3 1 175 1 207 1.60e-59 186
MS.gene24483.t1 AT3G55770 43.678 87 49 0 103 189 3 89 1.44e-15 72.8
MS.gene24483.t1 AT3G55770 48.201 139 61 3 1 135 1 132 2.16e-40 134
MS.gene24483.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.56e-17 74.3
MS.gene24483.t1 AT2G45800 45.455 154 76 3 34 184 4 152 4.96e-39 132
MS.gene24483.t1 AT2G45800 50.725 69 34 0 3 71 68 136 1.35e-16 74.7
MS.gene24483.t1 AT3G55770 48.462 130 57 2 1 126 1 124 1.92e-38 129
MS.gene24483.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.92e-17 73.9

Find 37 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTATCTATATTTCTTTC+TGG 0.117591 1.1:-4734463 None:intergenic
TCCCTCATGTGCTATGTAAT+TGG 0.275186 1.1:-4735023 None:intergenic
AGCATTTGAAACAACTCTTA+TGG 0.319973 1.1:-4734973 None:intergenic
TTTATAAAGGTTACAGTGAA+TGG 0.352930 1.1:+4734941 MS.gene24483:intron
TTTGCTTGATCAGTTGAATA+TGG 0.353364 1.1:-4735066 None:intergenic
GATCAACTGTTCAAAAGAAC+TGG 0.398776 1.1:+4734299 MS.gene24483:CDS
AGGAAAAGAATGGCATTTGC+AGG 0.400538 1.1:+4733694 None:intergenic
CTTGAGGCTTTAGCTGCTGC+AGG 0.425078 1.1:-4734581 None:intergenic
TATTCAACTGATCAAGCAAA+AGG 0.432443 1.1:+4735069 MS.gene24483:CDS
GCTAAAGCCTCAAGTATGTT+TGG 0.441559 1.1:+4734590 MS.gene24483:CDS
ACACTAATTACCTTCTCTGT+TGG 0.465114 1.1:-4734656 None:intergenic
GGAAATTTGAGCCAGCTTGA+AGG 0.475377 1.1:+4735091 MS.gene24483:CDS
GGAACAAGGGACAAATGTTC+TGG 0.477611 1.1:+4734614 MS.gene24483:CDS
TCTCATGGTCACCTTCAAGC+TGG 0.480325 1.1:-4735102 None:intergenic
CAAGTATGTTTGGTGGAACA+AGG 0.495604 1.1:+4734600 MS.gene24483:CDS
TTGAAACAACTCTTATGGTA+AGG 0.498185 1.1:-4734968 None:intergenic
AGAAAGAAATATAGATAATG+AGG 0.518674 1.1:+4734465 MS.gene24483:CDS
CCTTGAGGGTTCCTTTGCAG+TGG 0.520760 1.1:-4733816 None:intergenic
GAGAGAGACATAGACCTTGA+GGG 0.525903 1.1:-4733830 None:intergenic
GACTAATAGTACACCCTCCA+TGG 0.536605 1.1:-4734997 None:intergenic
AGGAACAACTCAGAAGTGTA+TGG 0.560932 1.1:+4733714 MS.gene24483:CDS
ATTCAACTGATCAAGCAAAA+GGG 0.562604 1.1:+4735070 MS.gene24483:CDS
AGTTGTTTCAAATGCTGCCA+TGG 0.564010 1.1:+4734980 MS.gene24483:CDS
GACATCTGAAACAAGCTTTG+TGG 0.565667 1.1:-4733792 None:intergenic
TTCCAATTACATAGCACATG+AGG 0.574672 1.1:+4735021 MS.gene24483:CDS
TTCAGATGTCACCACTGCAA+AGG 0.582786 1.1:+4733805 MS.gene24483:CDS
GCTTGATCAGTTGAATATGG+TGG 0.582930 1.1:-4735063 None:intergenic
AAAGCCTCAAGTATGTTTGG+TGG 0.587944 1.1:+4734593 MS.gene24483:CDS
GTTTCAAATGCTGCCATGGA+GGG 0.597804 1.1:+4734984 MS.gene24483:CDS
TTGAACAGTTGATCAAAGTG+TGG 0.606620 1.1:-4734290 None:intergenic
TGTTCCACCAAACATACTTG+AGG 0.606764 1.1:-4734597 None:intergenic
AACAGTGTATCCAACAGAGA+AGG 0.613878 1.1:+4734646 MS.gene24483:CDS
TCCAATTACATAGCACATGA+GGG 0.619052 1.1:+4735022 MS.gene24483:CDS
TCATGTGCTATGTAATTGGA+AGG 0.623562 1.1:-4735019 None:intergenic
AAGTATGTTTGGTGGAACAA+GGG 0.641097 1.1:+4734601 MS.gene24483:CDS
CCACTGCAAAGGAACCCTCA+AGG 0.664342 1.1:+4733816 MS.gene24483:CDS
TGTTTCAAATGCTGCCATGG+AGG 0.668776 1.1:+4734983 MS.gene24483:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTCAAAAATAATTTTGTT+CGG - chr1.1:4734806-4734825 None:intergenic 15.0%
!!! TCAATAATTTGCATTTATAA+AGG + chr1.1:4734928-4734947 MS.gene24483:intron 15.0%
!! AGAAAGAAATATAGATAATG+AGG + chr1.1:4734465-4734484 MS.gene24483:CDS 20.0%
!! GTTCATTAACATGAAAATTA+CGG - chr1.1:4734132-4734151 None:intergenic 20.0%
!! TCATTATCTATATTTCTTTC+TGG - chr1.1:4734466-4734485 None:intergenic 20.0%
! AAACAGCTCAAAAAATCATA+TGG - chr1.1:4734551-4734570 None:intergenic 25.0%
! AATGAACTTCTTTCATCATT+TGG + chr1.1:4734145-4734164 MS.gene24483:intron 25.0%
! AGGTAATGAATTCTAAATCT+AGG + chr1.1:4734485-4734504 MS.gene24483:intron 25.0%
! AGGTAATTAGTGTATATGTT+TGG + chr1.1:4734666-4734685 MS.gene24483:intron 25.0%
! CATAGCTTAAGTAAAAACTA+TGG + chr1.1:4734018-4734037 MS.gene24483:intron 25.0%
! GAAAATTGTTACTGCATATT+TGG - chr1.1:4734071-4734090 None:intergenic 25.0%
! TTAGTACATGTTTCAATAGA+CGG + chr1.1:4733945-4733964 MS.gene24483:intron 25.0%
! TTTATAAAGGTTACAGTGAA+TGG + chr1.1:4734941-4734960 MS.gene24483:intron 25.0%
!! AAGCAATTACAATTCTTTTG+AGG + chr1.1:4734250-4734269 MS.gene24483:CDS 25.0%
!! AGCAATTACAATTCTTTTGA+GGG + chr1.1:4734251-4734270 MS.gene24483:CDS 25.0%
!! AGTGTATATGTTTGGAATTA+CGG + chr1.1:4734674-4734693 MS.gene24483:intron 25.0%
!!! AAGTTTTGTATTCAACATCA+GGG - chr1.1:4734890-4734909 None:intergenic 25.0%
!!! TAAGTTTTGTATTCAACATC+AGG - chr1.1:4734891-4734910 None:intergenic 25.0%
!!! TGTAGCTTTTTCTAAAATCA+TGG + chr1.1:4734747-4734766 MS.gene24483:intron 25.0%
!!! TTTTGAGTTTGTGATTAGTT+TGG + chr1.1:4734817-4734836 MS.gene24483:intron 25.0%
AAAAATGCTGGGAAAATCAA+TGG + chr1.1:4735124-4735143 MS.gene24483:CDS 30.0%
ACCAAAAAGAAATGTCTAAG+AGG - chr1.1:4733860-4733879 None:intergenic 30.0%
ATTCAACTGATCAAGCAAAA+GGG + chr1.1:4735070-4735089 MS.gene24483:CDS 30.0%
CATTAACATGAAAATTACGG+TGG - chr1.1:4734129-4734148 None:intergenic 30.0%
TATTCAACTGATCAAGCAAA+AGG + chr1.1:4735069-4735088 MS.gene24483:CDS 30.0%
TGAAAATGCTTATGAACAAC+TGG - chr1.1:4734099-4734118 None:intergenic 30.0%
TTACTGCATATTTGGATTCA+CGG - chr1.1:4734063-4734082 None:intergenic 30.0%
TTGAAACAACTCTTATGGTA+AGG - chr1.1:4734971-4734990 None:intergenic 30.0%
TTTGCTTGATCAGTTGAATA+TGG - chr1.1:4735069-4735088 None:intergenic 30.0%
! ACCTCTTAGACATTTCTTTT+TGG + chr1.1:4733856-4733875 MS.gene24483:intron 30.0%
! AGCATTTGAAACAACTCTTA+TGG - chr1.1:4734976-4734995 None:intergenic 30.0%
! TTCTTTCATCATTTGGCTTA+TGG + chr1.1:4734152-4734171 MS.gene24483:intron 30.0%
!! AAAGCTTTGAAGGTAAACAA+AGG + chr1.1:4734333-4734352 MS.gene24483:intron 30.0%
!!! ACTTTTGTTTTGCATACCTA+GGG + chr1.1:4734422-4734441 MS.gene24483:intron 30.0%
!!! AGCTTTTTCTAAAATCATGG+TGG + chr1.1:4734750-4734769 MS.gene24483:intron 30.0%
!!! CTTTCTGGTTTGACAATTTT+TGG - chr1.1:4734451-4734470 None:intergenic 30.0%
!!! TACCTTCAAAGCTTTTTTCA+AGG - chr1.1:4734328-4734347 None:intergenic 30.0%
!!! TACTTTTGTTTTGCATACCT+AGG + chr1.1:4734421-4734440 MS.gene24483:intron 30.0%
ACACTAATTACCTTCTCTGT+TGG - chr1.1:4734659-4734678 None:intergenic 35.0%
CTAACAGATCCAATACTAGA+AGG - chr1.1:4733908-4733927 None:intergenic 35.0%
GATCAACTGTTCAAAAGAAC+TGG + chr1.1:4734299-4734318 MS.gene24483:CDS 35.0%
TCATGTGCTATGTAATTGGA+AGG - chr1.1:4735022-4735041 None:intergenic 35.0%
TCCAATTACATAGCACATGA+GGG + chr1.1:4735022-4735041 MS.gene24483:CDS 35.0%
TTCCAATTACATAGCACATG+AGG + chr1.1:4735021-4735040 MS.gene24483:CDS 35.0%
TTGAACAGTTGATCAAAGTG+TGG - chr1.1:4734293-4734312 None:intergenic 35.0%
! AAGTATGTTTGGTGGAACAA+GGG + chr1.1:4734601-4734620 MS.gene24483:CDS 35.0%
! AGCCTTGAAAAAAGCTTTGA+AGG + chr1.1:4734323-4734342 MS.gene24483:CDS 35.0%
! CTAGTTTAGCCTTCTAGTAT+TGG + chr1.1:4733896-4733915 MS.gene24483:intron 35.0%
! TTTCATCATTTGGCTTATGG+AGG + chr1.1:4734155-4734174 MS.gene24483:intron 35.0%
! TTTTCCCAGCATTTTTCTCA+TGG - chr1.1:4735120-4735139 None:intergenic 35.0%
!! CGGCTAGTTTAACAATATCA+CGG + chr1.1:4734694-4734713 MS.gene24483:intron 35.0%
AAAGCCTCAAGTATGTTTGG+TGG + chr1.1:4734593-4734612 MS.gene24483:CDS 40.0%
AACAGTGTATCCAACAGAGA+AGG + chr1.1:4734646-4734665 MS.gene24483:CDS 40.0%
GCTAAAGCCTCAAGTATGTT+TGG + chr1.1:4734590-4734609 MS.gene24483:CDS 40.0%
GCTTGATCAGTTGAATATGG+TGG - chr1.1:4735066-4735085 None:intergenic 40.0%
GTGACCATGAGAAAAATGCT+GGG + chr1.1:4735113-4735132 MS.gene24483:CDS 40.0%
GTTCGGATTTGTGATGAGTT+TGG - chr1.1:4734789-4734808 None:intergenic 40.0%
TCCCTCATGTGCTATGTAAT+TGG - chr1.1:4735026-4735045 None:intergenic 40.0%
TGCAACTTTCGCCAAAATCA+CGG - chr1.1:4734861-4734880 None:intergenic 40.0%
TGTTCCACCAAACATACTTG+AGG - chr1.1:4734600-4734619 None:intergenic 40.0%
! AGGAACAACTCAGAAGTGTA+TGG + chr1.1:4733714-4733733 MS.gene24483:CDS 40.0%
! AGTTGTTTCAAATGCTGCCA+TGG + chr1.1:4734980-4734999 MS.gene24483:CDS 40.0%
! CAAGTATGTTTGGTGGAACA+AGG + chr1.1:4734600-4734619 MS.gene24483:CDS 40.0%
! GACATCTGAAACAAGCTTTG+TGG - chr1.1:4733795-4733814 None:intergenic 40.0%
!!! TGACAATTTTTGGTGTCCCT+AGG - chr1.1:4734441-4734460 None:intergenic 40.0%
GACTAATAGTACACCCTCCA+TGG - chr1.1:4735000-4735019 None:intergenic 45.0%
GAGAGAGACATAGACCTTGA+GGG - chr1.1:4733833-4733852 None:intergenic 45.0%
GATGAGTTTGGCAGAATCTG+TGG - chr1.1:4734777-4734796 None:intergenic 45.0%
GGAAATTTGAGCCAGCTTGA+AGG + chr1.1:4735091-4735110 MS.gene24483:CDS 45.0%
GGAACAAGGGACAAATGTTC+TGG + chr1.1:4734614-4734633 MS.gene24483:CDS 45.0%
GGTGACCATGAGAAAAATGC+TGG + chr1.1:4735112-4735131 MS.gene24483:CDS 45.0%
GTTTCAAATGCTGCCATGGA+GGG + chr1.1:4734984-4735003 MS.gene24483:CDS 45.0%
TGAGAGAGACATAGACCTTG+AGG - chr1.1:4733834-4733853 None:intergenic 45.0%
TGTTTCAAATGCTGCCATGG+AGG + chr1.1:4734983-4735002 MS.gene24483:CDS 45.0%
TTCAGATGTCACCACTGCAA+AGG + chr1.1:4733805-4733824 MS.gene24483:CDS 45.0%
! TCATTGTGCGACCGTGATTT+TGG + chr1.1:4734847-4734866 MS.gene24483:intron 45.0%
TCTCATGGTCACCTTCAAGC+TGG - chr1.1:4735105-4735124 None:intergenic 50.0%
CCACTGCAAAGGAACCCTCA+AGG + chr1.1:4733816-4733835 MS.gene24483:CDS 55.0%
CCTTGAGGGTTCCTTTGCAG+TGG - chr1.1:4733819-4733838 None:intergenic 55.0%
! CTTGAGGCTTTAGCTGCTGC+AGG - chr1.1:4734584-4734603 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 4733703 4735171 4733703 ID=MS.gene24483
chr1.1 mRNA 4733703 4735171 4733703 ID=MS.gene24483.t1;Parent=MS.gene24483
chr1.1 exon 4733703 4733837 4733703 ID=MS.gene24483.t1.exon1;Parent=MS.gene24483.t1
chr1.1 CDS 4733703 4733837 4733703 ID=cds.MS.gene24483.t1;Parent=MS.gene24483.t1
chr1.1 exon 4734248 4734344 4734248 ID=MS.gene24483.t1.exon2;Parent=MS.gene24483.t1
chr1.1 CDS 4734248 4734344 4734248 ID=cds.MS.gene24483.t1;Parent=MS.gene24483.t1
chr1.1 exon 4734443 4734486 4734443 ID=MS.gene24483.t1.exon3;Parent=MS.gene24483.t1
chr1.1 CDS 4734443 4734486 4734443 ID=cds.MS.gene24483.t1;Parent=MS.gene24483.t1
chr1.1 exon 4734578 4734667 4734578 ID=MS.gene24483.t1.exon4;Parent=MS.gene24483.t1
chr1.1 CDS 4734578 4734667 4734578 ID=cds.MS.gene24483.t1;Parent=MS.gene24483.t1
chr1.1 exon 4734950 4735171 4734950 ID=MS.gene24483.t1.exon5;Parent=MS.gene24483.t1
chr1.1 CDS 4734950 4735171 4734950 ID=cds.MS.gene24483.t1;Parent=MS.gene24483.t1
Gene Sequence

>MS.gene24483

ATGGCATTTGCAGGAACAACTCAGAAGTGTATGGCTTGTAGCAAAACAGTTTATCTTGTTGATAAGTTAACTGCTGATAATAGAATTTTCCACAAAGCTTGTTTCAGATGTCACCACTGCAAAGGAACCCTCAAGGTCTATGTCTCTCTCATAACCTCTTAGACATTTCTTTTTGGTCTTATATATATAGTCTCTAGTTTAGCCTTCTAGTATTGGATCTGTTAGAATCATAATCATCAAATTTAGTACATGTTTCAATAGACGGTGAGTTTAGCGAAATGACAATAACACTGTAATTTTATTGAAGCTACAAAGCATAGCTTAAGTAAAAACTATGGTAGCACACAATGAAAATCGCCGTGAATCCAAATATGCAGTAACAATTTTCTTTAACCAGTTGTTCATAAGCATTTTCATCTCAGACCACCGTAATTTTCATGTTAATGAACTTCTTTCATCATTTGGCTTATGGAGGTATGTTGTTACTACATTGAAATAATTTTCATATACAAGTTTGCTGATAGATTAACGTATATACAATTCAGCTAAGCAATTACAATTCTTTTGAGGGAGTTCTTTACTGCAGACCACACTTTGATCAACTGTTCAAAAGAACTGGTAGCCTTGAAAAAAGCTTTGAAGGTAAACAAAGGATACATAATTGAATTTGTTAATTAAATGTCAACACAGTACTTTTTTCTTGTCAGTGTCTCAATTGTACTTTTGTTTTGCATACCTAGGGACACCAAAAATTGTCAAACCAGAAAGAAATATAGATAATGAGGTAATGAATTCTAAATCTAGGTGTTTACAATTCACAGTAGTAAATTCATGTTTTCAGCATTCCATATGATTTTTTGAGCTGTTTTAATCAGAAACCTGCAGCAGCTAAAGCCTCAAGTATGTTTGGTGGAACAAGGGACAAATGTTCTGGTTGTCAGAAAACAGTGTATCCAACAGAGAAGGTAATTAGTGTATATGTTTGGAATTACGGCTAGTTTAACAATATCACGGTGACACTGTAATTTTGTTGTAATTCTAAAGTGTAGCTTTTTCTAAAATCATGGTGGACCACAGATTCTGCCAAACTCATCACAAATCCGAACAAAATTATTTTTGAGTTTGTGATTAGTTTGGCAAAATCTCATTGTGCGACCGTGATTTTGGCGAAAGTTGCATCTAAACCCTGATGTTGAATACAAAACTTATTGTTCTACTAATGAAATCAATAATTTGCATTTATAAAGGTTACAGTGAATGGAACTCCTTACCATAAGAGTTGTTTCAAATGCTGCCATGGAGGGTGTACTATTAGTCCTTCCAATTACATAGCACATGAGGGAAAACTCTACTGCAAACACCACCATATTCAACTGATCAAGCAAAAGGGAAATTTGAGCCAGCTTGAAGGTGACCATGAGAAAAATGCTGGGAAAATCAATGGCGAAGAAGTTGCAGCTGAAACTTGA

Protein sequence

>MS.gene24483.t1

MAFAGTTQKCMACSKTVYLVDKLTADNRIFHKACFRCHHCKGTLKLSNYNSFEGVLYCRPHFDQLFKRTGSLEKSFEGTPKIVKPERNIDNEKPAAAKASSMFGGTRDKCSGCQKTVYPTEKVTVNGTPYHKSCFKCCHGGCTISPSNYIAHEGKLYCKHHHIQLIKQKGNLSQLEGDHEKNAGKINGEEVAAET