AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26109


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26109.t1 MTR_5g078330 73.659 205 8 2 1 160 1 204 1.81e-69 209
MS.gene26109.t1 MTR_3g065570 47.059 136 45 4 18 150 64 175 7.11e-17 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26109.t1 AT1G14900 41.611 149 54 4 17 154 73 199 4.59e-17 75.1

Find 68 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGTCTTGGGAGGTGCATT+AGG 0.195810 5.4:+56762836 None:intergenic
TCAGTCCTTTGAACCTTCTT+TGG 0.198015 5.4:+56762779 None:intergenic
CCTCTCTTTGGTGGTGCATT+TGG 0.255109 5.4:+56762953 None:intergenic
CTTCCACTTGAAGCAGCCTT+AGG 0.283817 5.4:+56762815 None:intergenic
GGTCTTCCACGTCCTCTCTT+TGG 0.323730 5.4:+56762941 None:intergenic
CTTAACCAGATGAAGGAAAA+TGG 0.327311 5.4:-56763016 MS.gene26109:intron
TTTGGTGGTGCATTTGGATC+AGG 0.346536 5.4:+56762959 None:intergenic
GCCAATGGATCCTTTGGCTT+TGG 0.350044 5.4:+56762917 None:intergenic
GGGTCTTGGGAGGTGCATTA+GGG 0.356682 5.4:+56762837 None:intergenic
TGAAGCAGCCTTAGGGGTCT+TGG 0.357314 5.4:+56762823 None:intergenic
AATGGATCCTTTGGCTTTGG+TGG 0.367305 5.4:+56762920 None:intergenic
TTCCACTTGAAGCAGCCTTA+GGG 0.394198 5.4:+56762816 None:intergenic
GGAGGTGCATTAGGGTCCTT+AGG 0.395197 5.4:+56762845 None:intergenic
GACGACCTCTTGGCCTTGGT+GGG 0.404356 5.4:+56762870 None:intergenic
GGTGGAGCCAATGGATCCTT+TGG 0.422272 5.4:+56762911 None:intergenic
GGAGGACGACCTCTTGGCCT+TGG 0.423053 5.4:+56762866 None:intergenic
CTTCCCCTTCCACTAGTAGT+TGG 0.429832 5.4:+56762653 None:intergenic
GGACGACCTCTTGGCCTTGG+TGG 0.430385 5.4:+56762869 None:intergenic
GAAGCAGCCTTAGGGGTCTT+GGG 0.435245 5.4:+56762824 None:intergenic
GACAGAACAGCAGCTGGTGG+TGG 0.460682 5.4:+56762893 None:intergenic
ACTACTAGTGGAAGGGGAAG+GGG 0.460928 5.4:-56762650 MS.gene26109:CDS
TCCTTAGGAGGACGACCTCT+TGG 0.478277 5.4:+56762860 None:intergenic
GTCATCTGAGGCTTAACCTT+AGG 0.479453 5.4:+56762620 None:intergenic
AACTACTAGTGGAAGGGGAA+GGG 0.479852 5.4:-56762651 MS.gene26109:CDS
TGTGAATTTACCTCAGGGTA+TGG 0.486285 5.4:+56763535 None:intergenic
CTTCCACGTCCTCTCTTTGG+TGG 0.517078 5.4:+56762944 None:intergenic
GCAGCTGGTGGTGGAGCCAA+TGG 0.520984 5.4:+56762902 None:intergenic
GGGAAGGGGTAGGCCTCCTA+AGG 0.528357 5.4:-56762636 MS.gene26109:CDS
AAGGCTGCTTCAAGTGGAAG+AGG 0.531029 5.4:-56762812 MS.gene26109:CDS
ACCCCTAAGGCTGCTTCAAG+TGG 0.532524 5.4:-56762818 MS.gene26109:CDS
GCCAAGAGGTCGTCCTCCTA+AGG 0.534326 5.4:-56762861 MS.gene26109:CDS
TCCACTTGAAGCAGCCTTAG+GGG 0.536454 5.4:+56762817 None:intergenic
ATCTGAGGCTTAACCTTAGG+AGG 0.537376 5.4:+56762623 None:intergenic
ACCAAAGCCAAAGGATCCAT+TGG 0.542885 5.4:-56762918 MS.gene26109:CDS
GCTTCAAGTGGAAGAGGAAG+GGG 0.550156 5.4:-56762806 MS.gene26109:CDS
GGTGGGGACAGAACAGCAGC+TGG 0.550403 5.4:+56762887 None:intergenic
TGCTTCAAGTGGAAGAGGAA+GGG 0.550902 5.4:-56762807 MS.gene26109:CDS
AGGAAGGGGTAGGCCAAAGA+AGG 0.551487 5.4:-56762792 MS.gene26109:CDS
ACAGCAACAGCAGCAACACT+TGG 0.556119 5.4:+56762677 None:intergenic
CAACTACTAGTGGAAGGGGA+AGG 0.556781 5.4:-56762652 MS.gene26109:CDS
GGGTATGGAGGAAGTGAAAG+AGG 0.561822 5.4:+56763550 None:intergenic
CTGCTTCAAGTGGAAGAGGA+AGG 0.565034 5.4:-56762808 MS.gene26109:CDS
AGCTTCAATGGCTACTCAAG+AGG 0.565718 5.4:-56763581 None:intergenic
TGCACCTCCCAAGACCCCTA+AGG 0.569439 5.4:-56762831 MS.gene26109:CDS
GCACCACCATCTCCACCACT+TGG 0.570283 5.4:+56762743 None:intergenic
CTGTTCCAACTACTAGTGGA+AGG 0.576494 5.4:-56762658 MS.gene26109:CDS
CTGCTGTTCTGTCCCCACCA+AGG 0.579342 5.4:-56762883 MS.gene26109:CDS
GTTCCAACTACTAGTGGAAG+GGG 0.585748 5.4:-56762656 MS.gene26109:CDS
TCAAATCCAAGTGGTGGAGA+TGG 0.596389 5.4:-56762749 MS.gene26109:CDS
GTTGCTGTTCCAACTACTAG+TGG 0.598369 5.4:-56762662 MS.gene26109:CDS
ACGACCTCTTGGCCTTGGTG+GGG 0.598499 5.4:+56762871 None:intergenic
GCACCACCAAAGAGAGGACG+TGG 0.602504 5.4:-56762947 MS.gene26109:CDS
AATGTTTCAAATCCAAGTGG+TGG 0.604254 5.4:-56762755 MS.gene26109:CDS
GGGGACAGAACAGCAGCTGG+TGG 0.609403 5.4:+56762890 None:intergenic
TGTTCCAACTACTAGTGGAA+GGG 0.611701 5.4:-56762657 MS.gene26109:CDS
GTAGGCCAAAGAAGGTTCAA+AGG 0.622028 5.4:-56762784 MS.gene26109:CDS
ACACTCACTTCAGTCATCTG+AGG 0.623459 5.4:+56762608 None:intergenic
GGTGCATTAGGGTCCTTAGG+AGG 0.623591 5.4:+56762848 None:intergenic
TGGAAGACCACCAAAGCCAA+AGG 0.624315 5.4:-56762927 MS.gene26109:CDS
GCAGCCTTAGGGGTCTTGGG+AGG 0.627198 5.4:+56762827 None:intergenic
GAATTTACCTCAGGGTATGG+AGG 0.638966 5.4:+56763538 None:intergenic
CCAAATGCACCACCAAAGAG+AGG 0.648545 5.4:-56762953 MS.gene26109:CDS
CTAGTGGAAGGGGAAGGGGT+AGG 0.654025 5.4:-56762646 MS.gene26109:CDS
AATCCAAGTGGTGGAGATGG+TGG 0.656042 5.4:-56762746 MS.gene26109:CDS
CTGTCCCCACCAAGGCCAAG+AGG 0.676490 5.4:-56762875 MS.gene26109:CDS
TTCACTTCCTCCATACCCTG+AGG 0.676529 5.4:-56763545 MS.gene26109:intron
GAGAATGTTTCAAATCCAAG+TGG 0.689463 5.4:-56762758 MS.gene26109:CDS
CAAGTGGAAGAGGAAGGGGT+AGG 0.697070 5.4:-56762802 MS.gene26109:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAAGGAAATTTTTTTTA+GGG - chr5.4:56762824-56762843 MS.gene26109:CDS 10.0%
!! TTCTTGAAAAAATAAGAAAA+GGG + chr5.4:56762683-56762702 None:intergenic 15.0%
!! TTTCTTGAAAAAATAAGAAA+AGG + chr5.4:56762684-56762703 None:intergenic 15.0%
!!! CAAATAAGGAAATTTTTTTT+AGG - chr5.4:56762823-56762842 MS.gene26109:CDS 15.0%
!! AAAATCATTCAACATGTTAA+TGG + chr5.4:56762936-56762955 None:intergenic 20.0%
!! AAATCATTCAACATGTTAAT+GGG + chr5.4:56762935-56762954 None:intergenic 20.0%
!! TCTTGAAAAAATAAGAAAAG+GGG + chr5.4:56762682-56762701 None:intergenic 20.0%
!!! TTAACAATTTTTACTCACTT+GGG - chr5.4:56762753-56762772 MS.gene26109:CDS 20.0%
!!! TTTAACAATTTTTACTCACT+TGG - chr5.4:56762752-56762771 MS.gene26109:CDS 20.0%
! AACTACATAGAATCAATCTA+TGG - chr5.4:56763093-56763112 MS.gene26109:intron 25.0%
! TCAAGATTACTATTTGGTTA+GGG + chr5.4:56762789-56762808 None:intergenic 25.0%
!! GATTTTGTAGATGATTATGA+AGG - chr5.4:56763020-56763039 MS.gene26109:intron 25.0%
!!! GCTGTTTTTGTAATCAAATA+AGG - chr5.4:56762809-56762828 MS.gene26109:CDS 25.0%
!!! TACAAAGACTTTTTTCTGTT+TGG + chr5.4:56762866-56762885 None:intergenic 25.0%
AACAGCTCAAGATTACTATT+TGG + chr5.4:56762795-56762814 None:intergenic 30.0%
AGTTGTTCTTTGCAAAAACA+AGG + chr5.4:56763185-56763204 None:intergenic 30.0%
CAAGATTACTATTTGGTTAG+GGG + chr5.4:56762788-56762807 None:intergenic 30.0%
CTCAAGATTACTATTTGGTT+AGG + chr5.4:56762790-56762809 None:intergenic 30.0%
GATTTGCAAGAACAATTGAA+TGG + chr5.4:56763134-56763153 None:intergenic 30.0%
! GTTGAATGATTTTCTCAACA+TGG - chr5.4:56762943-56762962 MS.gene26109:CDS 30.0%
!! GATGATTTGTTTGAACCATT+TGG + chr5.4:56763066-56763085 None:intergenic 30.0%
AAATCACCTTAACCAGATGA+AGG - chr5.4:56763149-56763168 MS.gene26109:intron 35.0%
AATGTTTCAAATCCAAGTGG+TGG - chr5.4:56763417-56763436 MS.gene26109:intron 35.0%
ATCTATGGTGAATTACCAGA+AGG - chr5.4:56763108-56763127 MS.gene26109:intron 35.0%
CTTAACCAGATGAAGGAAAA+TGG - chr5.4:56763156-56763175 MS.gene26109:intron 35.0%
GAGAATGTTTCAAATCCAAG+TGG - chr5.4:56763414-56763433 MS.gene26109:intron 35.0%
! AAAGGGGTGTATGTTGAATT+AGG + chr5.4:56762666-56762685 None:intergenic 35.0%
! AAGGGGTGTATGTTGAATTA+GGG + chr5.4:56762665-56762684 None:intergenic 35.0%
! CATTTTCCTTCATCTGGTTA+AGG + chr5.4:56763158-56763177 None:intergenic 35.0%
AGAACAATTGAATGGCCTTC+TGG + chr5.4:56763126-56763145 None:intergenic 40.0%
TCAGTCCTTTGAACCTTCTT+TGG + chr5.4:56763396-56763415 None:intergenic 40.0%
TGTGAATTTACCTCAGGGTA+TGG + chr5.4:56762640-56762659 None:intergenic 40.0%
TGTTCCAACTACTAGTGGAA+GGG - chr5.4:56763515-56763534 MS.gene26109:intron 40.0%
! AGGGGTGTATGTTGAATTAG+GGG + chr5.4:56762664-56762683 None:intergenic 40.0%
! GTAGATGATTATGAAGGCAC+TGG - chr5.4:56763026-56763045 MS.gene26109:intron 40.0%
!! GAAGCACTGAATGAACCAAA+TGG - chr5.4:56763048-56763067 MS.gene26109:intron 40.0%
AACTACTAGTGGAAGGGGAA+GGG - chr5.4:56763521-56763540 MS.gene26109:intron 45.0%
ACACTCACTTCAGTCATCTG+AGG + chr5.4:56763567-56763586 None:intergenic 45.0%
ACCAAAGCCAAAGGATCCAT+TGG - chr5.4:56763254-56763273 MS.gene26109:intron 45.0%
CTGTTCCAACTACTAGTGGA+AGG - chr5.4:56763514-56763533 MS.gene26109:intron 45.0%
GAATTTACCTCAGGGTATGG+AGG + chr5.4:56762637-56762656 None:intergenic 45.0%
GGGGTGTATGTTGAATTAGG+GGG + chr5.4:56762663-56762682 None:intergenic 45.0%
GTAGGCCAAAGAAGGTTCAA+AGG - chr5.4:56763388-56763407 MS.gene26109:intron 45.0%
GTTCCAACTACTAGTGGAAG+GGG - chr5.4:56763516-56763535 MS.gene26109:intron 45.0%
TGCTTCAAGTGGAAGAGGAA+GGG - chr5.4:56763365-56763384 MS.gene26109:intron 45.0%
TTCCACTTGAAGCAGCCTTA+GGG + chr5.4:56763359-56763378 None:intergenic 45.0%
! ATCTGAGGCTTAACCTTAGG+AGG + chr5.4:56763552-56763571 None:intergenic 45.0%
! GGTCACCATTTTCCTTCATC+TGG + chr5.4:56763164-56763183 None:intergenic 45.0%
! GTCATCTGAGGCTTAACCTT+AGG + chr5.4:56763555-56763574 None:intergenic 45.0%
! GTTGCTGTTCCAACTACTAG+TGG - chr5.4:56763510-56763529 MS.gene26109:intron 45.0%
! TCAAATCCAAGTGGTGGAGA+TGG - chr5.4:56763423-56763442 MS.gene26109:intron 45.0%
!! AATGGATCCTTTGGCTTTGG+TGG + chr5.4:56763255-56763274 None:intergenic 45.0%
!! TTTGGTGGTGCATTTGGATC+AGG + chr5.4:56763216-56763235 None:intergenic 45.0%
AAGGCTGCTTCAAGTGGAAG+AGG - chr5.4:56763360-56763379 MS.gene26109:intron 50.0%
ACAGCAACAGCAGCAACACT+TGG + chr5.4:56763498-56763517 None:intergenic 50.0%
ACTACTAGTGGAAGGGGAAG+GGG - chr5.4:56763522-56763541 MS.gene26109:intron 50.0%
CAACTACTAGTGGAAGGGGA+AGG - chr5.4:56763520-56763539 MS.gene26109:intron 50.0%
CCAAATGCACCACCAAAGAG+AGG - chr5.4:56763219-56763238 MS.gene26109:intron 50.0%
CTGCTTCAAGTGGAAGAGGA+AGG - chr5.4:56763364-56763383 MS.gene26109:intron 50.0%
CTTCCACTTGAAGCAGCCTT+AGG + chr5.4:56763360-56763379 None:intergenic 50.0%
CTTCCCCTTCCACTAGTAGT+TGG + chr5.4:56763522-56763541 None:intergenic 50.0%
GCTTCAAGTGGAAGAGGAAG+GGG - chr5.4:56763366-56763385 MS.gene26109:intron 50.0%
GGGTATGGAGGAAGTGAAAG+AGG + chr5.4:56762625-56762644 None:intergenic 50.0%
TCCACTTGAAGCAGCCTTAG+GGG + chr5.4:56763358-56763377 None:intergenic 50.0%
TGGAAGACCACCAAAGCCAA+AGG - chr5.4:56763245-56763264 MS.gene26109:intron 50.0%
TTCACTTCCTCCATACCCTG+AGG - chr5.4:56762627-56762646 MS.gene26109:CDS 50.0%
! AATCCAAGTGGTGGAGATGG+TGG - chr5.4:56763426-56763445 MS.gene26109:intron 50.0%
! AGGGGGTGTGAATTTACCTC+AGG + chr5.4:56762646-56762665 None:intergenic 50.0%
! GCCAATGGATCCTTTGGCTT+TGG + chr5.4:56763258-56763277 None:intergenic 50.0%
! GGGGGTGTGAATTTACCTCA+GGG + chr5.4:56762645-56762664 None:intergenic 50.0%
!! CCTCTCTTTGGTGGTGCATT+TGG + chr5.4:56763222-56763241 None:intergenic 50.0%
ACCCCTAAGGCTGCTTCAAG+TGG - chr5.4:56763354-56763373 MS.gene26109:intron 55.0%
AGGAAGGGGTAGGCCAAAGA+AGG - chr5.4:56763380-56763399 MS.gene26109:intron 55.0%
CAAGTGGAAGAGGAAGGGGT+AGG - chr5.4:56763370-56763389 MS.gene26109:intron 55.0%
CTTCCACGTCCTCTCTTTGG+TGG + chr5.4:56763231-56763250 None:intergenic 55.0%
GAAGCAGCCTTAGGGGTCTT+GGG + chr5.4:56763351-56763370 None:intergenic 55.0%
GGTCTTCCACGTCCTCTCTT+TGG + chr5.4:56763234-56763253 None:intergenic 55.0%
GGTGCATTAGGGTCCTTAGG+AGG + chr5.4:56763327-56763346 None:intergenic 55.0%
GGTGGAGCCAATGGATCCTT+TGG + chr5.4:56763264-56763283 None:intergenic 55.0%
TCCTTAGGAGGACGACCTCT+TGG + chr5.4:56763315-56763334 None:intergenic 55.0%
TGAAGCAGCCTTAGGGGTCT+TGG + chr5.4:56763352-56763371 None:intergenic 55.0%
! GGAGGTGCATTAGGGTCCTT+AGG + chr5.4:56763330-56763349 None:intergenic 55.0%
! GGGTCTTGGGAGGTGCATTA+GGG + chr5.4:56763338-56763357 None:intergenic 55.0%
CTAGTGGAAGGGGAAGGGGT+AGG - chr5.4:56763526-56763545 MS.gene26109:intron 60.0%
CTGCTGTTCTGTCCCCACCA+AGG - chr5.4:56763289-56763308 MS.gene26109:intron 60.0%
GACAGAACAGCAGCTGGTGG+TGG + chr5.4:56763282-56763301 None:intergenic 60.0%
GCACCACCAAAGAGAGGACG+TGG - chr5.4:56763225-56763244 MS.gene26109:intron 60.0%
GCACCACCATCTCCACCACT+TGG + chr5.4:56763432-56763451 None:intergenic 60.0%
GCCAAGAGGTCGTCCTCCTA+AGG - chr5.4:56763311-56763330 MS.gene26109:intron 60.0%
TGCACCTCCCAAGACCCCTA+AGG - chr5.4:56763341-56763360 MS.gene26109:intron 60.0%
! GGGGTCTTGGGAGGTGCATT+AGG + chr5.4:56763339-56763358 None:intergenic 60.0%
!! ACGACCTCTTGGCCTTGGTG+GGG + chr5.4:56763304-56763323 None:intergenic 60.0%
!! GACGACCTCTTGGCCTTGGT+GGG + chr5.4:56763305-56763324 None:intergenic 60.0%
CTGTCCCCACCAAGGCCAAG+AGG - chr5.4:56763297-56763316 MS.gene26109:intron 65.0%
GCAGCCTTAGGGGTCTTGGG+AGG + chr5.4:56763348-56763367 None:intergenic 65.0%
GGAGGACGACCTCTTGGCCT+TGG + chr5.4:56763309-56763328 None:intergenic 65.0%
GGGAAGGGGTAGGCCTCCTA+AGG - chr5.4:56763536-56763555 MS.gene26109:intron 65.0%
GGGGACAGAACAGCAGCTGG+TGG + chr5.4:56763285-56763304 None:intergenic 65.0%
GGTGGGGACAGAACAGCAGC+TGG + chr5.4:56763288-56763307 None:intergenic 65.0%
TGCTGCTGCTGCTGCTGCTG+AGG - chr5.4:56763449-56763468 MS.gene26109:intron 65.0%
!! GCAGCTGGTGGTGGAGCCAA+TGG + chr5.4:56763273-56763292 None:intergenic 65.0%
!! GGACGACCTCTTGGCCTTGG+TGG + chr5.4:56763306-56763325 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 56762598 56763596 56762598 ID=MS.gene26109
chr5.4 mRNA 56762598 56763596 56762598 ID=MS.gene26109.t1;Parent=MS.gene26109
chr5.4 exon 56763546 56763596 56763546 ID=MS.gene26109.t1.exon1;Parent=MS.gene26109.t1
chr5.4 CDS 56763546 56763596 56763546 ID=cds.MS.gene26109.t1;Parent=MS.gene26109.t1
chr5.4 exon 56762598 56763029 56762598 ID=MS.gene26109.t1.exon2;Parent=MS.gene26109.t1
chr5.4 CDS 56762598 56763029 56762598 ID=cds.MS.gene26109.t1;Parent=MS.gene26109.t1
Gene Sequence

>MS.gene26109

ATGGCTACTCAAGAGGTTAATAAGCCTCTTTCACTTCCTCCATACCCTGAGGTAAATTCACACCCCCTAATTCAACATACACCCCTTTTCTTATTTTTTCAAGAAAATTAGTCTTTCATAGTAAAAGTAGCAATTTTTTCACTGATCTGTTTGATTTAACAATTTTTACTCACTTGGGTACTTTTTTCCCCTAACCAAATAGTAATCTTGAGCTGTTTTTGTAATCAAATAAGGAAATTTTTTTTAGGGTTTATGAAGAATTTAACCAAACAGAAAAAAGTCTTTGTATTAATTTCAGCTCAATTAATCTTGAAATTCTTTGTTTTTTCACTAACCCATTAACATGTTGAATGATTTTCTCAACATGGTATCTTCATTTCTGAAAAAAATTTGATTTTTATTTTTATTTTAACTCTGTTGTTGATTTTGTAGATGATTATGAAGGCACTGGAAGCACTGAATGAACCAAATGGTTCAAACAAATCATCAATTTCAAACTACATAGAATCAATCTATGGTGAATTACCAGAAGGCCATTCAATTGTTCTTGCAAATCACCTTAACCAGATGAAGGAAAATGGTGACCTTGTTTTTGCAAAGAACAACTACATGAAGCCTGATCCAAATGCACCACCAAAGAGAGGACGTGGAAGACCACCAAAGCCAAAGGATCCATTGGCTCCACCACCAGCTGCTGTTCTGTCCCCACCAAGGCCAAGAGGTCGTCCTCCTAAGGACCCTAATGCACCTCCCAAGACCCCTAAGGCTGCTTCAAGTGGAAGAGGAAGGGGTAGGCCAAAGAAGGTTCAAAGGACTGAGAATGTTTCAAATCCAAGTGGTGGAGATGGTGGTGCTGCTGCTGCTGCTGCTGCTGAGGTTGATGATGATGATGCTGCTCCAAGTGTTGCTGCTGTTGCTGTTCCAACTACTAGTGGAAGGGGAAGGGGTAGGCCTCCTAAGGTTAAGCCTCAGATGACTGAAGTGAGTGTTGAATCATAG

Protein sequence

>MS.gene26109.t1

MATQEVNKPLSLPPYPEMKENGDLVFAKNNYMKPDPNAPPKRGRGRPPKPKDPLAPPPAAVLSPPRPRGRPPKDPNAPPKTPKAASSGRGRGRPKKVQRTENVSNPSGGDGGAAAAAAAEVDDDDAAPSVAAVAVPTTSGRGRGRPPKVKPQMTEVSVES