Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26267.t1 | XP_024639172.1 | 100 | 77 | 0 | 0 | 1 | 77 | 34 | 110 | 3.00E-36 | 160.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26267.t1 | Q54UU1 | 50.0 | 76 | 37 | 1 | 4 | 78 | 3 | 78 | 5.6e-15 | 81.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26267.t1 | A0A072UGD7 | 100.0 | 77 | 0 | 0 | 1 | 77 | 34 | 110 | 2.2e-36 | 160.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049756 | MS.gene26267 | 0.824034 | 1.00E-53 | -1.69E-46 |
| MS.gene050277 | MS.gene26267 | 0.818939 | 1.51E-52 | -1.69E-46 |
| MS.gene050317 | MS.gene26267 | 0.844031 | 9.73E-59 | -1.69E-46 |
| MS.gene050320 | MS.gene26267 | 0.809536 | 1.80E-50 | -1.69E-46 |
| MS.gene051063 | MS.gene26267 | 0.80991 | 1.50E-50 | -1.69E-46 |
| MS.gene051245 | MS.gene26267 | 0.825681 | 4.11E-54 | -1.69E-46 |
| MS.gene051746 | MS.gene26267 | 0.809576 | 1.77E-50 | -1.69E-46 |
| MS.gene051748 | MS.gene26267 | 0.832082 | 1.16E-55 | -1.69E-46 |
| MS.gene051756 | MS.gene26267 | 0.804058 | 2.59E-49 | -1.69E-46 |
| MS.gene051757 | MS.gene26267 | 0.805805 | 1.12E-49 | -1.69E-46 |
| MS.gene051903 | MS.gene26267 | 0.819248 | 1.28E-52 | -1.69E-46 |
| MS.gene052286 | MS.gene26267 | 0.800507 | 1.40E-48 | -1.69E-46 |
| MS.gene052302 | MS.gene26267 | 0.807462 | 5.00E-50 | -1.69E-46 |
| MS.gene052376 | MS.gene26267 | 0.802211 | 6.25E-49 | -1.69E-46 |
| MS.gene052856 | MS.gene26267 | 0.830073 | 3.61E-55 | -1.69E-46 |
| MS.gene053109 | MS.gene26267 | 0.825039 | 5.83E-54 | -1.69E-46 |
| MS.gene053437 | MS.gene26267 | 0.816945 | 4.26E-52 | -1.69E-46 |
| MS.gene053952 | MS.gene26267 | 0.800942 | 1.14E-48 | -1.69E-46 |
| MS.gene055174 | MS.gene26267 | 0.813269 | 2.79E-51 | -1.69E-46 |
| MS.gene055441 | MS.gene26267 | 0.808488 | 3.02E-50 | -1.69E-46 |
| MS.gene056432 | MS.gene26267 | 0.818743 | 1.67E-52 | -1.69E-46 |
| MS.gene056631 | MS.gene26267 | 0.803831 | 2.89E-49 | -1.69E-46 |
| MS.gene056760 | MS.gene26267 | 0.802559 | 5.30E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene26267 | 0.838334 | 3.06E-57 | -1.69E-46 |
| MS.gene057094 | MS.gene26267 | 0.82568 | 4.11E-54 | -1.69E-46 |
| MS.gene057234 | MS.gene26267 | 0.813683 | 2.26E-51 | -1.69E-46 |
| MS.gene057452 | MS.gene26267 | -0.811136 | 8.14E-51 | -1.69E-46 |
| MS.gene058182 | MS.gene26267 | 0.851979 | 6.23E-61 | -1.69E-46 |
| MS.gene058238 | MS.gene26267 | 0.839079 | 1.96E-57 | -1.69E-46 |
| MS.gene058906 | MS.gene26267 | 0.840421 | 8.78E-58 | -1.69E-46 |
| MS.gene059171 | MS.gene26267 | 0.81285 | 3.45E-51 | -1.69E-46 |
| MS.gene06001 | MS.gene26267 | 0.821246 | 4.47E-53 | -1.69E-46 |
| MS.gene060869 | MS.gene26267 | 0.807966 | 3.90E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26267.t1 | MTR_5g075330 | 100.000 | 77 | 0 | 0 | 1 | 77 | 1 | 77 | 3.13e-53 | 162 |
| MS.gene26267.t1 | MTR_5g075330 | 100.000 | 77 | 0 | 0 | 1 | 77 | 34 | 110 | 5.90e-53 | 162 |
| MS.gene26267.t1 | MTR_8g103680 | 94.805 | 77 | 4 | 0 | 1 | 77 | 73 | 149 | 2.39e-51 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26267.t1 | AT5G02280 | 89.610 | 77 | 8 | 0 | 1 | 77 | 1 | 77 | 6.65e-48 | 149 |
Find 16 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCAGTTATCACCTGTTTC+AGG | 0.153521 | 5.4:+59128532 | MS.gene26267:CDS |
| TCACCTGTTTCAGGTTGTTC+AGG | 0.323200 | 5.4:+59128541 | MS.gene26267:CDS |
| CATTGCTTCCAATCGCTAAC+AGG | 0.330687 | 5.4:+59128598 | MS.gene26267:CDS |
| TGGTGGATTGATCTACTATA+AGG | 0.415123 | 5.4:+59127768 | MS.gene26267:CDS |
| CCTTACGAGTGGCTAGCTTA+TGG | 0.418783 | 5.4:+59128488 | MS.gene26267:CDS |
| ATTCCTGAACAACCTGAAAC+AGG | 0.450702 | 5.4:-59128544 | None:intergenic |
| CTTTACATCATCAATAAGTC+TGG | 0.473288 | 5.4:+59127748 | MS.gene26267:CDS |
| ATTCAGGATTATGGGTCGGC+TGG | 0.489577 | 5.4:+59128445 | MS.gene26267:intron |
| AAGTAATACCTGTTAGCGAT+TGG | 0.512163 | 5.4:-59128606 | None:intergenic |
| GAAGATCAAATGTGTCAGCT+TGG | 0.530412 | 5.4:-59128576 | None:intergenic |
| TTATGGGTCGGCTGGACGAA+TGG | 0.537132 | 5.4:+59128453 | MS.gene26267:CDS |
| TGATATTCAGGATTATGGGT+CGG | 0.544902 | 5.4:+59128441 | MS.gene26267:intron |
| TACATCATCAATAAGTCTGG+TGG | 0.561761 | 5.4:+59127751 | MS.gene26267:CDS |
| CCATAAGCTAGCCACTCGTA+AGG | 0.587798 | 5.4:-59128488 | None:intergenic |
| AGGTGATAACTGCTGAGAGA+TGG | 0.596514 | 5.4:-59128524 | None:intergenic |
| TACAAATGATACCTTACGAG+TGG | 0.655543 | 5.4:+59128477 | MS.gene26267:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTTATATACTAAAAAACAA+CGG | - | chr5.4:59127985-59128004 | None:intergenic | 15.0% |
| !! | TAATATTAATCAAGATTTCA+AGG | - | chr5.4:59128268-59128287 | None:intergenic | 15.0% |
| !! | TAATGTTCAATACAAAATTT+AGG | - | chr5.4:59128130-59128149 | None:intergenic | 15.0% |
| !!! | AATTTTTTTAGATTGAATCA+TGG | - | chr5.4:59127941-59127960 | None:intergenic | 15.0% |
| !!! | TATATAAGTTTTGTTAAGTT+AGG | + | chr5.4:59127996-59128015 | MS.gene26267:intron | 15.0% |
| !! | AGATATATTCTACTCTAAAT+TGG | + | chr5.4:59127843-59127862 | MS.gene26267:intron | 20.0% |
| !! | ATACTGAAATCTCAAAAAAA+AGG | - | chr5.4:59128024-59128043 | None:intergenic | 20.0% |
| !! | CTTATATACTAAAAAACAAC+GGG | - | chr5.4:59127984-59128003 | None:intergenic | 20.0% |
| !! | GATTTGAAAAAATTCTACTA+AGG | + | chr5.4:59128228-59128247 | MS.gene26267:intron | 20.0% |
| !! | TGAATCATAGTATGATAATT+AGG | + | chr5.4:59128059-59128078 | MS.gene26267:intron | 20.0% |
| !!! | TGTTAAACTTTTTGATATTC+AGG | + | chr5.4:59128429-59128448 | MS.gene26267:intron | 20.0% |
| !!! | TTTTTGATATTCAGGATTAT+GGG | + | chr5.4:59128437-59128456 | MS.gene26267:intron | 20.0% |
| !!! | TTTTTTGTATTAGATGATAC+TGG | - | chr5.4:59128339-59128358 | None:intergenic | 20.0% |
| !! | CTTTTTGATATTCAGGATTA+TGG | + | chr5.4:59128436-59128455 | MS.gene26267:intron | 25.0% |
| AACAACGGGATAAAATACAA+AGG | - | chr5.4:59127970-59127989 | None:intergenic | 30.0% | |
| AATTAGGATATGACTTCAGT+TGG | + | chr5.4:59128075-59128094 | MS.gene26267:intron | 30.0% | |
| CTTTACATCATCAATAAGTC+TGG | + | chr5.4:59127748-59127767 | MS.gene26267:CDS | 30.0% | |
| TTGTTAATTGGAGTGAGTTT+CGG | + | chr5.4:59127892-59127911 | MS.gene26267:intron | 30.0% | |
| AAGTAATACCTGTTAGCGAT+TGG | - | chr5.4:59128609-59128628 | None:intergenic | 35.0% | |
| AGCAGTTTAAGAAACCACAA+TGG | + | chr5.4:59128287-59128306 | MS.gene26267:intron | 35.0% | |
| AGTTGTATTTGCGAAGCTTA+GGG | + | chr5.4:59128203-59128222 | MS.gene26267:intron | 35.0% | |
| TACAAATGATACCTTACGAG+TGG | + | chr5.4:59128477-59128496 | MS.gene26267:CDS | 35.0% | |
| TACATCATCAATAAGTCTGG+TGG | + | chr5.4:59127751-59127770 | MS.gene26267:CDS | 35.0% | |
| TGATATTCAGGATTATGGGT+CGG | + | chr5.4:59128441-59128460 | MS.gene26267:intron | 35.0% | |
| TGGATTAAATGATGCCATTG+TGG | - | chr5.4:59128304-59128323 | None:intergenic | 35.0% | |
| ! | TGAGTTAGAGCAATGTGTTT+TGG | + | chr5.4:59128381-59128400 | MS.gene26267:intron | 35.0% |
| !! | GATACTGGTTCTAACAGATA+TGG | - | chr5.4:59128324-59128343 | None:intergenic | 35.0% |
| !! | TGGTGGATTGATCTACTATA+AGG | + | chr5.4:59127768-59127787 | MS.gene26267:CDS | 35.0% |
| ATTCCTGAACAACCTGAAAC+AGG | - | chr5.4:59128547-59128566 | None:intergenic | 40.0% | |
| GAAGATCAAATGTGTCAGCT+TGG | - | chr5.4:59128579-59128598 | None:intergenic | 40.0% | |
| GAGTTGTATTTGCGAAGCTT+AGG | + | chr5.4:59128202-59128221 | MS.gene26267:intron | 40.0% | |
| ! | GAGCATGTTGGGTTGTTAAT+TGG | + | chr5.4:59127880-59127899 | MS.gene26267:intron | 40.0% |
| AGGTGATAACTGCTGAGAGA+TGG | - | chr5.4:59128527-59128546 | None:intergenic | 45.0% | |
| CAGCAGTTATCACCTGTTTC+AGG | + | chr5.4:59128532-59128551 | MS.gene26267:CDS | 45.0% | |
| CATTGCTTCCAATCGCTAAC+AGG | + | chr5.4:59128598-59128617 | MS.gene26267:CDS | 45.0% | |
| CTTCGCTTCATGAGCATGTT+GGG | + | chr5.4:59127869-59127888 | MS.gene26267:intron | 45.0% | |
| GGATATGACTTCAGTTGGCA+AGG | + | chr5.4:59128080-59128099 | MS.gene26267:intron | 45.0% | |
| TATGACTTCAGTTGGCAAGG+TGG | + | chr5.4:59128083-59128102 | MS.gene26267:intron | 45.0% | |
| TCTTCGCTTCATGAGCATGT+TGG | + | chr5.4:59127868-59127887 | MS.gene26267:intron | 45.0% | |
| !! | TCACCTGTTTCAGGTTGTTC+AGG | + | chr5.4:59128541-59128560 | MS.gene26267:CDS | 45.0% |
| ATTCAGGATTATGGGTCGGC+TGG | + | chr5.4:59128445-59128464 | MS.gene26267:intron | 50.0% | |
| CCATAAGCTAGCCACTCGTA+AGG | - | chr5.4:59128491-59128510 | None:intergenic | 50.0% | |
| !! | CCTTACGAGTGGCTAGCTTA+TGG | + | chr5.4:59128488-59128507 | MS.gene26267:CDS | 50.0% |
| TTATGGGTCGGCTGGACGAA+TGG | + | chr5.4:59128453-59128472 | MS.gene26267:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 59127730 | 59128636 | 59127730 | ID=MS.gene26267 |
| chr5.4 | mRNA | 59127730 | 59128636 | 59127730 | ID=MS.gene26267.t1;Parent=MS.gene26267 |
| chr5.4 | exon | 59127730 | 59127789 | 59127730 | ID=MS.gene26267.t1.exon1;Parent=MS.gene26267.t1 |
| chr5.4 | CDS | 59127730 | 59127789 | 59127730 | ID=cds.MS.gene26267.t1;Parent=MS.gene26267.t1 |
| chr5.4 | exon | 59128451 | 59128636 | 59128451 | ID=MS.gene26267.t1.exon2;Parent=MS.gene26267.t1 |
| chr5.4 | CDS | 59128451 | 59128636 | 59128451 | ID=cds.MS.gene26267.t1;Parent=MS.gene26267.t1 |
| Gene Sequence |
| Protein sequence |
