AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26351


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26351.t1 MTR_4g088515 96.642 268 9 0 1 268 1 268 0.0 540
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26351.t1 AT4G26415 44.872 234 62 10 3 192 7 217 1.04e-43 150

Find 60 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCCTCAAGAACTCTT+TGG 0.228130 4.1:+21490911 MS.gene26351:CDS
TCAATGAATATAGTGGAATA+AGG 0.272127 4.1:+21491180 MS.gene26351:CDS
TGGTGTTGTGGCAAAGTTAA+TGG 0.280238 4.1:+21491436 MS.gene26351:CDS
GCCATTTGAGAGGCATGATT+TGG 0.298125 4.1:+21491556 MS.gene26351:CDS
TCCATGTATAGGTCATCTTT+TGG 0.313265 4.1:+21491296 MS.gene26351:CDS
TCTTATGCCTTTCTTCATCT+TGG 0.319608 4.1:-21490937 None:intergenic
TTTCTCCTCAAGAACTCTTT+GGG 0.349291 4.1:+21490912 MS.gene26351:CDS
AACCAACAAAATAGTACTAT+TGG 0.366556 4.1:+21490996 MS.gene26351:CDS
TGGATATTGTATTATGAAAC+CGG 0.367697 4.1:+21491661 MS.gene26351:CDS
GGATGAGTAGTGTTGATTGT+AGG 0.385703 4.1:+21491513 MS.gene26351:CDS
CAATGAATATAGTGGAATAA+GGG 0.392950 4.1:+21491181 MS.gene26351:CDS
AGAACCAAAGCTTGATGAAA+AGG 0.407802 4.1:+21491361 MS.gene26351:CDS
GCCATAGCTTCTAGAGAAAC+CGG 0.424661 4.1:-21491680 None:intergenic
GGTGTTGTGGCAAAGTTAAT+GGG 0.432813 4.1:+21491437 MS.gene26351:CDS
TGTGGCAAAGTTAATGGGTT+TGG 0.433784 4.1:+21491442 MS.gene26351:CDS
TCTTATAGTACAATTAGAAA+TGG 0.439711 4.1:+21491130 MS.gene26351:CDS
CTCCTTGGTTGCCAACTACT+AGG 0.457255 4.1:-21491710 None:intergenic
ACTATAAGATCTTCTAATGT+TGG 0.471701 4.1:-21491116 None:intergenic
GTTAGAAGAAGAAATAGGTC+TGG 0.472977 4.1:+21491623 MS.gene26351:CDS
TCCAAATCATGCCTCTCAAA+TGG 0.478781 4.1:-21491557 None:intergenic
TGCTGCTTGTGATGTCAACT+TGG 0.485632 4.1:-21491040 None:intergenic
ATCTAAACATATCTCCTCCT+TGG 0.489207 4.1:-21491725 None:intergenic
AGTACTATTGGCAATGGCAT+TGG 0.489373 4.1:+21491008 MS.gene26351:CDS
AAAACTAGCAATGGACATGC+AGG 0.491726 4.1:+21491583 MS.gene26351:CDS
CCCAAAAGATGACCTATACA+TGG 0.498041 4.1:-21491297 None:intergenic
TTGTGATGTCAACTTGGCTA+TGG 0.503039 4.1:-21491034 None:intergenic
TTCTCCCCAGCAACTGTTGG+TGG 0.503702 4.1:-21491395 None:intergenic
GTAGGAGACAAAATATAAGA+AGG 0.511223 4.1:+21491531 MS.gene26351:CDS
ATGGAAGAGATTCCATGTAT+AGG 0.512387 4.1:+21491285 MS.gene26351:CDS
ATTAACTTTGCCACAACACC+AGG 0.513967 4.1:-21491434 None:intergenic
TGGCAGGAGGTCCTAGTAGT+TGG 0.521625 4.1:+21491699 MS.gene26351:CDS
ACCGGTTTCTCTAGAAGCTA+TGG 0.522607 4.1:+21491679 MS.gene26351:CDS
TTGCTGGGGAGAACGTGGTT+TGG 0.527912 4.1:+21491405 MS.gene26351:CDS
ACATTGTTAGAAGAAGAAAT+AGG 0.529346 4.1:+21491618 MS.gene26351:CDS
ATGCTTTGAATCAATATCCA+AGG 0.531501 4.1:+21491252 MS.gene26351:CDS
AAATGGAGGAAGAGATGGCA+AGG 0.533678 4.1:+21491147 MS.gene26351:CDS
ATTAGAAATGGAGGAAGAGA+TGG 0.541356 4.1:+21491142 MS.gene26351:CDS
GAGTTCTTGAGGAGAAAGAA+TGG 0.549925 4.1:-21490906 None:intergenic
TTGGGAGCCAAGATGAAGAA+AGG 0.561593 4.1:+21490930 MS.gene26351:CDS
CAAAATAGTACTATTGGCAA+TGG 0.563916 4.1:+21491002 MS.gene26351:CDS
CTTCCATCAAGTGAAAACCT+TGG 0.575355 4.1:-21491269 None:intergenic
TATAGTACAATTAGAAATGG+AGG 0.575642 4.1:+21491133 MS.gene26351:CDS
GTCCTAGTAGTTGGCAACCA+AGG 0.577078 4.1:+21491708 MS.gene26351:CDS
AATGAATATAGTGGAATAAG+GGG 0.579962 4.1:+21491182 MS.gene26351:CDS
TGGCTCCCAAAGAGTTCTTG+AGG 0.597986 4.1:-21490917 None:intergenic
GAAGCTATTCCAGTGTCAGT+AGG 0.605402 4.1:+21491464 MS.gene26351:CDS
TTTGGAGAGAAAACTAGCAA+TGG 0.605568 4.1:+21491574 MS.gene26351:CDS
AACGTGGTTTGGTGTAAACC+TGG 0.608504 4.1:+21491416 MS.gene26351:CDS
GTTTCTCTAGAAGCTATGGC+AGG 0.610709 4.1:+21491683 MS.gene26351:CDS
AACAGTTGCTGGGGAGAACG+TGG 0.612915 4.1:+21491400 MS.gene26351:CDS
AATATAGTGGAATAAGGGGA+AGG 0.620558 4.1:+21491186 MS.gene26351:CDS
GGAGACAAAATATAAGAAGG+AGG 0.626425 4.1:+21491534 MS.gene26351:CDS
TAAGAAGGAGGCCATTTGAG+AGG 0.627512 4.1:+21491546 MS.gene26351:CDS
CTGACATATTAAGATCTGCA+AGG 0.639107 4.1:+21491228 MS.gene26351:CDS
AGTTGACATCACAAGCAGCA+AGG 0.656859 4.1:+21491043 MS.gene26351:CDS
ACGTTCTCCCCAGCAACTGT+TGG 0.657823 4.1:-21491398 None:intergenic
TCTCTAGAAGCTATGGCAGG+AGG 0.665560 4.1:+21491686 MS.gene26351:CDS
TTGGTGTAAACCTGGTGTTG+TGG 0.669247 4.1:+21491424 MS.gene26351:CDS
AAACTAGCAATGGACATGCA+GGG 0.676573 4.1:+21491584 MS.gene26351:CDS
CTAGTAGTTGGCAACCAAGG+AGG 0.699039 4.1:+21491711 MS.gene26351:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTATAGTACAATTAGAAA+TGG + chr4.1:21491130-21491149 MS.gene26351:CDS 20.0%
! AACCAACAAAATAGTACTAT+TGG + chr4.1:21490996-21491015 MS.gene26351:CDS 25.0%
! ACATTGTTAGAAGAAGAAAT+AGG + chr4.1:21491618-21491637 MS.gene26351:CDS 25.0%
! ACTATAAGATCTTCTAATGT+TGG - chr4.1:21491119-21491138 None:intergenic 25.0%
! GAAAAACTCAATGAATATAG+TGG + chr4.1:21491173-21491192 MS.gene26351:CDS 25.0%
! TATAGTACAATTAGAAATGG+AGG + chr4.1:21491133-21491152 MS.gene26351:CDS 25.0%
! TGGATATTGTATTATGAAAC+CGG + chr4.1:21491661-21491680 MS.gene26351:CDS 25.0%
!! AATGAATATAGTGGAATAAG+GGG + chr4.1:21491182-21491201 MS.gene26351:CDS 25.0%
!! CAATGAATATAGTGGAATAA+GGG + chr4.1:21491181-21491200 MS.gene26351:CDS 25.0%
!! TCAATGAATATAGTGGAATA+AGG + chr4.1:21491180-21491199 MS.gene26351:CDS 25.0%
AAAGATCTGACAAAAAAGAG+AGG + chr4.1:21491492-21491511 MS.gene26351:CDS 30.0%
AGTTGTCACATTGCAAAAAA+GGG - chr4.1:21491076-21491095 None:intergenic 30.0%
CAAAATAGTACTATTGGCAA+TGG + chr4.1:21491002-21491021 MS.gene26351:CDS 30.0%
GTAGGAGACAAAATATAAGA+AGG + chr4.1:21491531-21491550 MS.gene26351:CDS 30.0%
! ATAACCTTTTCATCAAGCTT+TGG - chr4.1:21491368-21491387 None:intergenic 30.0%
! ATGCTTTGAATCAATATCCA+AGG + chr4.1:21491252-21491271 MS.gene26351:CDS 30.0%
! TGCCAATAGTACTATTTTGT+TGG - chr4.1:21491001-21491020 None:intergenic 30.0%
!!! ATTTTGTTGGTTTAGAGTTG+AGG - chr4.1:21490988-21491007 None:intergenic 30.0%
!!! TCTGGTTTTTGCTCTAAAAA+TGG + chr4.1:21491641-21491660 MS.gene26351:CDS 30.0%
AGAACCAAAGCTTGATGAAA+AGG + chr4.1:21491361-21491380 MS.gene26351:CDS 35.0%
ATGGAAGAGATTCCATGTAT+AGG + chr4.1:21491285-21491304 MS.gene26351:CDS 35.0%
ATTAGAAATGGAGGAAGAGA+TGG + chr4.1:21491142-21491161 MS.gene26351:CDS 35.0%
CTGACATATTAAGATCTGCA+AGG + chr4.1:21491228-21491247 MS.gene26351:CDS 35.0%
GAGTTGTCACATTGCAAAAA+AGG - chr4.1:21491077-21491096 None:intergenic 35.0%
GGAGACAAAATATAAGAAGG+AGG + chr4.1:21491534-21491553 MS.gene26351:CDS 35.0%
GTTAGAAGAAGAAATAGGTC+TGG + chr4.1:21491623-21491642 MS.gene26351:CDS 35.0%
GTTGTCACATTGCAAAAAAG+GGG - chr4.1:21491075-21491094 None:intergenic 35.0%
TCTTATGCCTTTCTTCATCT+TGG - chr4.1:21490940-21490959 None:intergenic 35.0%
TTTCTCCTCAAGAACTCTTT+GGG + chr4.1:21490912-21490931 MS.gene26351:CDS 35.0%
TTTGGAGAGAAAACTAGCAA+TGG + chr4.1:21491574-21491593 MS.gene26351:CDS 35.0%
! AATATAGTGGAATAAGGGGA+AGG + chr4.1:21491186-21491205 MS.gene26351:CDS 35.0%
! CCATGTATAGGTCATCTTTT+GGG + chr4.1:21491297-21491316 MS.gene26351:CDS 35.0%
! TATCCAAGGTTTTCACTTGA+TGG + chr4.1:21491266-21491285 MS.gene26351:CDS 35.0%
! TCCATGTATAGGTCATCTTT+TGG + chr4.1:21491296-21491315 MS.gene26351:CDS 35.0%
!!! TCATCTTTTGGGACTATAGA+AGG + chr4.1:21491308-21491327 MS.gene26351:CDS 35.0%
AAAACTAGCAATGGACATGC+AGG + chr4.1:21491583-21491602 MS.gene26351:CDS 40.0%
AAACTAGCAATGGACATGCA+GGG + chr4.1:21491584-21491603 MS.gene26351:CDS 40.0%
AGTACTATTGGCAATGGCAT+TGG + chr4.1:21491008-21491027 MS.gene26351:CDS 40.0%
ATTAACTTTGCCACAACACC+AGG - chr4.1:21491437-21491456 None:intergenic 40.0%
CCCAAAAGATGACCTATACA+TGG - chr4.1:21491300-21491319 None:intergenic 40.0%
CTTCCATCAAGTGAAAACCT+TGG - chr4.1:21491272-21491291 None:intergenic 40.0%
CTTTCTCCTCAAGAACTCTT+TGG + chr4.1:21490911-21490930 MS.gene26351:CDS 40.0%
GAGTTCTTGAGGAGAAAGAA+TGG - chr4.1:21490909-21490928 None:intergenic 40.0%
TCCAAATCATGCCTCTCAAA+TGG - chr4.1:21491560-21491579 None:intergenic 40.0%
TTGTGATGTCAACTTGGCTA+TGG - chr4.1:21491037-21491056 None:intergenic 40.0%
! CTTTTTCTACCTACTGACAC+TGG - chr4.1:21491476-21491495 None:intergenic 40.0%
! GGTGTTGTGGCAAAGTTAAT+GGG + chr4.1:21491437-21491456 MS.gene26351:CDS 40.0%
!! GGATGAGTAGTGTTGATTGT+AGG + chr4.1:21491513-21491532 MS.gene26351:CDS 40.0%
!! TGGTGTTGTGGCAAAGTTAA+TGG + chr4.1:21491436-21491455 MS.gene26351:CDS 40.0%
!! TGTGGCAAAGTTAATGGGTT+TGG + chr4.1:21491442-21491461 MS.gene26351:CDS 40.0%
AAATGGAGGAAGAGATGGCA+AGG + chr4.1:21491147-21491166 MS.gene26351:CDS 45.0%
AGTTGACATCACAAGCAGCA+AGG + chr4.1:21491043-21491062 MS.gene26351:CDS 45.0%
GAAGCTATTCCAGTGTCAGT+AGG + chr4.1:21491464-21491483 MS.gene26351:CDS 45.0%
TAAGAAGGAGGCCATTTGAG+AGG + chr4.1:21491546-21491565 MS.gene26351:CDS 45.0%
TTGGGAGCCAAGATGAAGAA+AGG + chr4.1:21490930-21490949 MS.gene26351:CDS 45.0%
! ACCGGTTTCTCTAGAAGCTA+TGG + chr4.1:21491679-21491698 MS.gene26351:CDS 45.0%
! GCCATAGCTTCTAGAGAAAC+CGG - chr4.1:21491683-21491702 None:intergenic 45.0%
! GCCATTTGAGAGGCATGATT+TGG + chr4.1:21491556-21491575 MS.gene26351:CDS 45.0%
! GTTTCTCTAGAAGCTATGGC+AGG + chr4.1:21491683-21491702 MS.gene26351:CDS 45.0%
! GTTTTCCACCAACAGTTGCT+GGG + chr4.1:21491390-21491409 MS.gene26351:CDS 45.0%
! TGCTGCTTGTGATGTCAACT+TGG - chr4.1:21491043-21491062 None:intergenic 45.0%
! TGTTTTCCACCAACAGTTGC+TGG + chr4.1:21491389-21491408 MS.gene26351:CDS 45.0%
! TTGGTGTAAACCTGGTGTTG+TGG + chr4.1:21491424-21491443 MS.gene26351:CDS 45.0%
! TTTTCCACCAACAGTTGCTG+GGG + chr4.1:21491391-21491410 MS.gene26351:CDS 45.0%
!! AACGTGGTTTGGTGTAAACC+TGG + chr4.1:21491416-21491435 MS.gene26351:CDS 45.0%
CTAGTAGTTGGCAACCAAGG+AGG + chr4.1:21491711-21491730 MS.gene26351:CDS 50.0%
CTCCTTGGTTGCCAACTACT+AGG - chr4.1:21491713-21491732 None:intergenic 50.0%
GTCCTAGTAGTTGGCAACCA+AGG + chr4.1:21491708-21491727 MS.gene26351:CDS 50.0%
TGGCTCCCAAAGAGTTCTTG+AGG - chr4.1:21490920-21490939 None:intergenic 50.0%
! TCTCTAGAAGCTATGGCAGG+AGG + chr4.1:21491686-21491705 MS.gene26351:CDS 50.0%
AACAGTTGCTGGGGAGAACG+TGG + chr4.1:21491400-21491419 MS.gene26351:CDS 55.0%
ACGTTCTCCCCAGCAACTGT+TGG - chr4.1:21491401-21491420 None:intergenic 55.0%
TGGCAGGAGGTCCTAGTAGT+TGG + chr4.1:21491699-21491718 MS.gene26351:CDS 55.0%
TTCTCCCCAGCAACTGTTGG+TGG - chr4.1:21491398-21491417 None:intergenic 55.0%
TTGCTGGGGAGAACGTGGTT+TGG + chr4.1:21491405-21491424 MS.gene26351:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 21490903 21491745 21490903 ID=MS.gene26351
chr4.1 mRNA 21490903 21491745 21490903 ID=MS.gene26351.t1;Parent=MS.gene26351
chr4.1 exon 21490903 21491745 21490903 ID=MS.gene26351.t1.exon1;Parent=MS.gene26351.t1
chr4.1 CDS 21490903 21491745 21490903 ID=cds.MS.gene26351.t1;Parent=MS.gene26351.t1
Gene Sequence

>MS.gene26351

ATGCCATTCTTTCTCCTCAAGAACTCTTTGGGAGCCAAGATGAAGAAAGGCATAAGAACTTTCTGCAACACTGATGTGTCAACCTCAACTCTAAACCAACAAAATAGTACTATTGGCAATGGCATTGGCAACCATAGCCAAGTTGACATCACAAGCAGCAAGGTAAGCTCCCCTTTTTTGCAATGTGACAACTCAAATTCAAAGCAAAGCACACCAACATTAGAAGATCTTATAGTACAATTAGAAATGGAGGAAGAGATGGCAAGGAAAGAAAAACTCAATGAATATAGTGGAATAAGGGGAAGGATGTCATGTGTTAACAACTCTGACATATTAAGATCTGCAAGGAATGCTTTGAATCAATATCCAAGGTTTTCACTTGATGGAAGAGATTCCATGTATAGGTCATCTTTTGGGACTATAGAAGGAAGAAGATCAGTTTGTAGTGAGATAAGTTTAGAACCAAAGCTTGATGAAAAGGTTATGTGTTTTCCACCAACAGTTGCTGGGGAGAACGTGGTTTGGTGTAAACCTGGTGTTGTGGCAAAGTTAATGGGTTTGGAAGCTATTCCAGTGTCAGTAGGTAGAAAAAGATCTGACAAAAAAGAGAGGATGAGTAGTGTTGATTGTAGGAGACAAAATATAAGAAGGAGGCCATTTGAGAGGCATGATTTGGAGAGAAAACTAGCAATGGACATGCAGGGTTATCGTGATCACATTGTTAGAAGAAGAAATAGGTCTGGTTTTTGCTCTAAAAATGGATATTGTATTATGAAACCGGTTTCTCTAGAAGCTATGGCAGGAGGTCCTAGTAGTTGGCAACCAAGGAGGAGATATGTTTAG

Protein sequence

>MS.gene26351.t1

MPFFLLKNSLGAKMKKGIRTFCNTDVSTSTLNQQNSTIGNGIGNHSQVDITSSKVSSPFLQCDNSNSKQSTPTLEDLIVQLEMEEEMARKEKLNEYSGIRGRMSCVNNSDILRSARNALNQYPRFSLDGRDSMYRSSFGTIEGRRSVCSEISLEPKLDEKVMCFPPTVAGENVVWCKPGVVAKLMGLEAIPVSVGRKRSDKKERMSSVDCRRQNIRRRPFERHDLERKLAMDMQGYRDHIVRRRNRSGFCSKNGYCIMKPVSLEAMAGGPSSWQPRRRYV