Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28354.t1 | XP_013456272.1 | 98.8 | 584 | 7 | 0 | 1 | 584 | 1 | 584 | 0.00E+00 | 1127.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28354.t1 | Q84RR0 | 73.9 | 529 | 136 | 2 | 1 | 528 | 1 | 528 | 7.7e-239 | 827.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28354.t1 | A0A072UML4 | 98.8 | 584 | 7 | 0 | 1 | 584 | 1 | 584 | 0.0e+00 | 1127.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04919 | MS.gene28354 | 0.803725 | 3.04E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene28354 | 0.817389 | 3.38E-52 | -1.69E-46 |
| MS.gene049283 | MS.gene28354 | 0.843903 | 1.05E-58 | -1.69E-46 |
| MS.gene049411 | MS.gene28354 | 0.802295 | 6.00E-49 | -1.69E-46 |
| MS.gene049499 | MS.gene28354 | 0.813834 | 2.09E-51 | -1.69E-46 |
| MS.gene050085 | MS.gene28354 | 0.803687 | 3.09E-49 | -1.69E-46 |
| MS.gene050386 | MS.gene28354 | 0.801011 | 1.10E-48 | -1.69E-46 |
| MS.gene050414 | MS.gene28354 | 0.820657 | 6.11E-53 | -1.69E-46 |
| MS.gene050646 | MS.gene28354 | 0.856641 | 2.80E-62 | -1.69E-46 |
| MS.gene050945 | MS.gene28354 | 0.888912 | 3.87E-73 | -1.69E-46 |
| MS.gene051043 | MS.gene28354 | 0.83647 | 9.19E-57 | -1.69E-46 |
| MS.gene051343 | MS.gene28354 | 0.817633 | 2.98E-52 | -1.69E-46 |
| MS.gene051597 | MS.gene28354 | 0.806348 | 8.59E-50 | -1.69E-46 |
| MS.gene051699 | MS.gene28354 | 0.805676 | 1.19E-49 | -1.69E-46 |
| MS.gene051902 | MS.gene28354 | 0.821595 | 3.71E-53 | -1.69E-46 |
| MS.gene052072 | MS.gene28354 | 0.874403 | 6.94E-68 | -1.69E-46 |
| MS.gene052533 | MS.gene28354 | 0.800035 | 1.74E-48 | -1.69E-46 |
| MS.gene052793 | MS.gene28354 | 0.806429 | 8.26E-50 | -1.69E-46 |
| MS.gene053931 | MS.gene28354 | 0.808333 | 3.26E-50 | -1.69E-46 |
| MS.gene054004 | MS.gene28354 | 0.810312 | 1.23E-50 | -1.69E-46 |
| MS.gene054413 | MS.gene28354 | 0.865272 | 6.66E-65 | -1.69E-46 |
| MS.gene054709 | MS.gene28354 | 0.815275 | 1.00E-51 | -1.69E-46 |
| MS.gene054957 | MS.gene28354 | 0.820755 | 5.80E-53 | -1.69E-46 |
| MS.gene056578 | MS.gene28354 | 0.818476 | 1.92E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene28354 | 0.809986 | 1.44E-50 | -1.69E-46 |
| MS.gene057174 | MS.gene28354 | 0.808957 | 2.40E-50 | -1.69E-46 |
| MS.gene057622 | MS.gene28354 | 0.805933 | 1.05E-49 | -1.69E-46 |
| MS.gene05781 | MS.gene28354 | 0.800093 | 1.69E-48 | -1.69E-46 |
| MS.gene058074 | MS.gene28354 | 0.838099 | 3.52E-57 | -1.69E-46 |
| MS.gene058781 | MS.gene28354 | 0.81274 | 3.64E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene28354 | 0.836793 | 7.60E-57 | -1.69E-46 |
| MS.gene05985 | MS.gene28354 | -0.821376 | 4.17E-53 | -1.69E-46 |
| MS.gene060244 | MS.gene28354 | 0.804412 | 2.19E-49 | -1.69E-46 |
| MS.gene060933 | MS.gene28354 | 0.863112 | 3.14E-64 | -1.69E-46 |
| MS.gene060935 | MS.gene28354 | 0.844155 | 9.01E-59 | -1.69E-46 |
| MS.gene060937 | MS.gene28354 | 0.858344 | 8.79E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28354.t1 | MTR_4g066650 | 98.801 | 584 | 7 | 0 | 1 | 584 | 1 | 584 | 0.0 | 1215 |
| MS.gene28354.t1 | MTR_2g098580 | 80.268 | 598 | 102 | 4 | 1 | 584 | 1 | 596 | 0.0 | 967 |
| MS.gene28354.t1 | MTR_3g065380 | 71.160 | 586 | 153 | 6 | 1 | 582 | 1 | 574 | 0.0 | 840 |
| MS.gene28354.t1 | MTR_3g065410 | 69.244 | 582 | 149 | 5 | 1 | 582 | 1 | 552 | 0.0 | 792 |
| MS.gene28354.t1 | MTR_5g097670 | 84.566 | 311 | 48 | 0 | 223 | 533 | 4 | 314 | 0.0 | 559 |
| MS.gene28354.t1 | MTR_4g075010 | 40.586 | 478 | 215 | 9 | 57 | 519 | 2 | 425 | 9.79e-117 | 358 |
| MS.gene28354.t1 | MTR_4g075030 | 39.785 | 372 | 183 | 11 | 38 | 395 | 10 | 354 | 9.47e-78 | 251 |
| MS.gene28354.t1 | MTR_4g068940 | 29.423 | 537 | 336 | 13 | 18 | 529 | 2 | 520 | 1.36e-64 | 222 |
| MS.gene28354.t1 | MTR_4g068940 | 29.423 | 537 | 336 | 13 | 18 | 529 | 2 | 520 | 1.99e-64 | 222 |
| MS.gene28354.t1 | MTR_4g096720 | 53.030 | 66 | 28 | 1 | 243 | 305 | 58 | 123 | 2.27e-16 | 77.4 |
| MS.gene28354.t1 | MTR_3g462590 | 24.359 | 234 | 166 | 7 | 102 | 329 | 62 | 290 | 7.44e-14 | 73.2 |
| MS.gene28354.t1 | MTR_3g462570 | 24.561 | 228 | 151 | 9 | 124 | 343 | 33 | 247 | 8.35e-12 | 68.6 |
| MS.gene28354.t1 | MTR_8g022900 | 25.837 | 209 | 136 | 8 | 137 | 332 | 63 | 265 | 2.60e-11 | 65.1 |
| MS.gene28354.t1 | MTR_2g044440 | 25.263 | 190 | 134 | 5 | 129 | 313 | 88 | 274 | 2.65e-11 | 65.1 |
| MS.gene28354.t1 | MTR_5g078410 | 24.771 | 218 | 138 | 6 | 117 | 329 | 134 | 330 | 3.41e-11 | 65.5 |
| MS.gene28354.t1 | MTR_1g105460 | 29.787 | 188 | 118 | 7 | 154 | 333 | 121 | 302 | 4.31e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28354.t1 | AT2G31510 | 72.887 | 568 | 138 | 5 | 1 | 559 | 1 | 561 | 0.0 | 847 |
| MS.gene28354.t1 | AT1G65430 | 69.338 | 574 | 159 | 4 | 18 | 584 | 4 | 567 | 0.0 | 823 |
| MS.gene28354.t1 | AT2G31510 | 76.892 | 489 | 109 | 2 | 74 | 559 | 2 | 489 | 0.0 | 792 |
| MS.gene28354.t1 | AT2G31510 | 74.206 | 504 | 118 | 3 | 1 | 498 | 1 | 498 | 0.0 | 792 |
| MS.gene28354.t1 | AT1G05890 | 70.385 | 520 | 138 | 4 | 20 | 533 | 16 | 525 | 0.0 | 768 |
| MS.gene28354.t1 | AT1G05890 | 73.684 | 475 | 122 | 2 | 59 | 533 | 96 | 567 | 0.0 | 754 |
| MS.gene28354.t1 | AT2G31770 | 56.673 | 517 | 195 | 8 | 16 | 522 | 3 | 500 | 0.0 | 599 |
| MS.gene28354.t1 | AT2G31760 | 59.140 | 465 | 171 | 6 | 57 | 519 | 40 | 487 | 0.0 | 582 |
| MS.gene28354.t1 | AT2G31780 | 55.677 | 458 | 179 | 8 | 1 | 451 | 1 | 441 | 0.0 | 520 |
| MS.gene28354.t1 | AT1G05880 | 39.407 | 472 | 253 | 8 | 61 | 523 | 40 | 487 | 8.35e-108 | 333 |
| MS.gene28354.t1 | AT1G05880 | 39.195 | 472 | 247 | 10 | 61 | 523 | 40 | 480 | 2.46e-103 | 321 |
| MS.gene28354.t1 | AT1G05880 | 38.546 | 454 | 246 | 8 | 61 | 505 | 40 | 469 | 2.01e-97 | 305 |
| MS.gene28354.t1 | AT4G34370 | 30.490 | 551 | 330 | 18 | 19 | 535 | 2 | 533 | 8.93e-68 | 231 |
| MS.gene28354.t1 | AT2G16090 | 29.744 | 585 | 341 | 18 | 19 | 559 | 2 | 560 | 8.25e-64 | 220 |
| MS.gene28354.t1 | AT2G16090 | 29.188 | 579 | 346 | 16 | 19 | 559 | 2 | 554 | 1.08e-62 | 217 |
| MS.gene28354.t1 | AT2G16090 | 30.286 | 525 | 313 | 15 | 19 | 516 | 2 | 500 | 1.56e-62 | 216 |
| MS.gene28354.t1 | AT3G27710 | 29.412 | 476 | 294 | 14 | 62 | 516 | 45 | 499 | 5.43e-59 | 206 |
| MS.gene28354.t1 | AT2G16090 | 29.865 | 519 | 302 | 15 | 82 | 559 | 3 | 500 | 1.25e-58 | 205 |
| MS.gene28354.t1 | AT5G63750 | 29.541 | 501 | 297 | 15 | 57 | 519 | 6 | 488 | 2.33e-58 | 204 |
| MS.gene28354.t1 | AT2G16090 | 30.501 | 459 | 274 | 12 | 82 | 516 | 3 | 440 | 2.55e-57 | 200 |
| MS.gene28354.t1 | AT5G08730 | 27.484 | 473 | 307 | 10 | 58 | 523 | 6 | 449 | 4.42e-56 | 197 |
| MS.gene28354.t1 | AT5G63730 | 27.190 | 548 | 315 | 18 | 52 | 580 | 4 | 486 | 2.74e-50 | 181 |
| MS.gene28354.t1 | AT4G34370 | 33.333 | 378 | 207 | 14 | 187 | 535 | 5 | 366 | 9.54e-48 | 172 |
| MS.gene28354.t1 | AT3G27720 | 32.047 | 337 | 190 | 10 | 199 | 516 | 143 | 459 | 8.36e-43 | 160 |
| MS.gene28354.t1 | AT5G63760 | 27.711 | 498 | 270 | 21 | 113 | 580 | 2 | 439 | 1.13e-42 | 159 |
| MS.gene28354.t1 | AT5G63760 | 27.711 | 498 | 270 | 21 | 113 | 580 | 2 | 439 | 1.13e-42 | 159 |
| MS.gene28354.t1 | AT5G63760 | 27.711 | 498 | 270 | 21 | 113 | 580 | 2 | 439 | 1.13e-42 | 159 |
| MS.gene28354.t1 | AT5G63760 | 28.021 | 389 | 207 | 15 | 208 | 580 | 2 | 333 | 1.15e-33 | 131 |
| MS.gene28354.t1 | AT5G10370 | 26.693 | 251 | 170 | 9 | 87 | 330 | 1512 | 1755 | 7.15e-12 | 69.3 |
| MS.gene28354.t1 | AT2G26135 | 26.291 | 213 | 139 | 8 | 121 | 325 | 140 | 342 | 1.88e-11 | 66.6 |
| MS.gene28354.t1 | AT3G45580 | 29.469 | 207 | 124 | 10 | 136 | 329 | 172 | 369 | 2.25e-11 | 66.6 |
Find 128 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAGCAAAATTCAAATTTAC+AGG | 0.232442 | 4.2:+38814869 | None:intergenic |
| ATCGACGTTGATTGCAAATC+TGG | 0.244897 | 4.2:+38811249 | None:intergenic |
| TTCGATTGGTCGCTTGCATT+TGG | 0.271459 | 4.2:+38814929 | None:intergenic |
| GATATCAGATTCCTTCAATA+TGG | 0.313478 | 4.2:+38817352 | None:intergenic |
| TATCTCACCTCGCTAGTATT+AGG | 0.330743 | 4.2:+38816633 | None:intergenic |
| TGGCCCACCGCTCATAATAA+TGG | 0.335297 | 4.2:+38814256 | None:intergenic |
| CCCACTTGTGGCGCTGCTAT+TGG | 0.344918 | 4.2:-38816095 | MS.gene28354:CDS |
| GGGAGCAGTAAGGGGAGAGC+TGG | 0.349500 | 4.2:-38811371 | MS.gene28354:CDS |
| AGTCATTGAATTCTTTAGAT+TGG | 0.359218 | 4.2:+38811624 | None:intergenic |
| TCGATTGGTCGCTTGCATTT+GGG | 0.365336 | 4.2:+38814930 | None:intergenic |
| TGTCATCTTCCTGTCTTACT+TGG | 0.369228 | 4.2:+38817330 | None:intergenic |
| GTCACAGCTTAAGTTTATTA+CGG | 0.378239 | 4.2:-38812122 | MS.gene28354:CDS |
| CCGAAAAGCGGTTGGCTTGT+TGG | 0.378590 | 4.2:-38816676 | MS.gene28354:CDS |
| CTATCTGATGTAGATGCTAA+TGG | 0.382104 | 4.2:-38811434 | MS.gene28354:CDS |
| TATGCACGATGCTCACGATA+TGG | 0.384878 | 4.2:-38818241 | MS.gene28354:CDS |
| TACTACTTCGTCACCACAAT+TGG | 0.386991 | 4.2:-38817259 | MS.gene28354:intron |
| GGGAGAGCTGGGAGAGGAAA+GGG | 0.387635 | 4.2:-38811359 | MS.gene28354:CDS |
| TTATATCGAAGACAATAAAA+AGG | 0.396653 | 4.2:-38816003 | MS.gene28354:intron |
| ATCCATACTGTTTCTCATGC+TGG | 0.403926 | 4.2:-38816438 | MS.gene28354:CDS |
| GTATTAGGATGCTGGACAAC+TGG | 0.406004 | 4.2:+38816648 | None:intergenic |
| AACTATCCTCGCTCTGGGAT+TGG | 0.407143 | 4.2:-38816478 | MS.gene28354:CDS |
| TATCTGATGTAGATGCTAAT+GGG | 0.411020 | 4.2:-38811433 | MS.gene28354:CDS |
| GACTTTCGTACAAAATTAGC+TGG | 0.416189 | 4.2:-38811605 | MS.gene28354:CDS |
| GCAGCGCCACAAGTGGGTTC+AGG | 0.416212 | 4.2:+38816101 | None:intergenic |
| TCCCAGCATGAGAAACAGTA+TGG | 0.418225 | 4.2:+38816436 | None:intergenic |
| TTTGAAAACTATCCTCGCTC+TGG | 0.436202 | 4.2:-38816484 | MS.gene28354:CDS |
| GGGGAGAGCTGGGAGAGGAA+AGG | 0.438486 | 4.2:-38811360 | MS.gene28354:CDS |
| TTAGTGAGGGCTTTAGAGAA+TGG | 0.439616 | 4.2:-38811458 | MS.gene28354:CDS |
| GGCCCACCGCTCATAATAAT+GGG | 0.441437 | 4.2:+38814257 | None:intergenic |
| CTGAAATGGACGTATGCATA+TGG | 0.442573 | 4.2:-38811866 | MS.gene28354:CDS |
| TACTGAAAGAAGAAGGGAAA+TGG | 0.451324 | 4.2:-38814304 | MS.gene28354:CDS |
| GGTCGCTTGCATTTGGGACA+TGG | 0.453953 | 4.2:+38814936 | None:intergenic |
| TGTAGAAATCGTCATCTTCA+AGG | 0.456356 | 4.2:+38818214 | None:intergenic |
| CACTGGACGATGAGCCTCCT+CGG | 0.458483 | 4.2:+38815476 | None:intergenic |
| ATGATGATACTGAAAGAAGA+AGG | 0.463836 | 4.2:-38814311 | MS.gene28354:CDS |
| GGAGCAGTAAGGGGAGAGCT+GGG | 0.464600 | 4.2:-38811370 | MS.gene28354:CDS |
| ACATCCAGGACCATCATTGA+TGG | 0.478158 | 4.2:+38816139 | None:intergenic |
| AGCAGCTAAGCAAGAGGGAG+TGG | 0.478756 | 4.2:-38814425 | MS.gene28354:intron |
| TCCATACTGTTTCTCATGCT+GGG | 0.479243 | 4.2:-38816437 | MS.gene28354:CDS |
| AGTGTAGGAGAGTGCTGAAA+TGG | 0.479640 | 4.2:-38811880 | MS.gene28354:CDS |
| ATAAGATCTACGAAGGTAAC+GGG | 0.483111 | 4.2:+38816022 | None:intergenic |
| TCAAAGTGGTGTCCTGCCCC+TGG | 0.492940 | 4.2:-38815679 | MS.gene28354:CDS |
| CAGAGTCTGAAAACATGAAC+TGG | 0.493565 | 4.2:-38815413 | MS.gene28354:intron |
| GATACATACAGATTGGTTGC+TGG | 0.496836 | 4.2:+38814236 | None:intergenic |
| ATCTGATATCCAAGTAAGAC+AGG | 0.497385 | 4.2:-38817339 | MS.gene28354:CDS |
| AGGTGAAGCAGAATCTGGTC+TGG | 0.499622 | 4.2:-38811702 | MS.gene28354:CDS |
| TATTACGGAGGCCTGGCTGC+AGG | 0.501896 | 4.2:-38812107 | MS.gene28354:intron |
| ACCAATAGCAGCGCCACAAG+TGG | 0.504223 | 4.2:+38816094 | None:intergenic |
| ATAAACTTAAGCTGTGACTC+AGG | 0.505087 | 4.2:+38812127 | None:intergenic |
| GGAAATGGCGAAGAATTCAT+TGG | 0.506032 | 4.2:-38814289 | MS.gene28354:CDS |
| TACTGTTTCTCATGCTGGGA+AGG | 0.514636 | 4.2:-38816433 | MS.gene28354:intron |
| TATCTACATAGGTTATGCCT+TGG | 0.517708 | 4.2:-38814505 | MS.gene28354:intron |
| GAAATTGAATTTACCAATTG+TGG | 0.519364 | 4.2:+38817246 | None:intergenic |
| AAATGCTGCAGGGAGCAGTA+AGG | 0.522523 | 4.2:-38811381 | MS.gene28354:CDS |
| CCTCGCTAGTATTAGGATGC+TGG | 0.523850 | 4.2:+38816640 | None:intergenic |
| AGCATCGTGCATATCATCTT+CGG | 0.523950 | 4.2:+38818251 | None:intergenic |
| CGATTTCTACAGTGGTGATA+CGG | 0.524021 | 4.2:-38818202 | MS.gene28354:CDS |
| TTGAAAACTATCCTCGCTCT+GGG | 0.528875 | 4.2:-38816483 | MS.gene28354:CDS |
| ACCACAGTGCCACAGTCCAC+TGG | 0.533681 | 4.2:+38815459 | None:intergenic |
| TTAAGTTTATTACGGAGGCC+TGG | 0.533852 | 4.2:-38812114 | MS.gene28354:CDS |
| CAGCAATTCCTCAGTGCTGA+AGG | 0.541611 | 4.2:-38811647 | MS.gene28354:CDS |
| CAAAATTAGCTGGATTGACT+AGG | 0.541759 | 4.2:-38811595 | MS.gene28354:intron |
| ACGTCCATCAATGATGGTCC+TGG | 0.548432 | 4.2:-38816143 | MS.gene28354:CDS |
| TGAGCTCGTTGCTTTGCTGA+AGG | 0.552429 | 4.2:+38811406 | None:intergenic |
| ATCAGCACGTCCATCAATGA+TGG | 0.552763 | 4.2:-38816149 | MS.gene28354:CDS |
| CAAATCATTTACCTGCAGCC+AGG | 0.554241 | 4.2:+38812096 | None:intergenic |
| ATAGGTTATGCCTTGGTGCA+TGG | 0.554336 | 4.2:-38814498 | MS.gene28354:intron |
| TGATGATACTGAAAGAAGAA+GGG | 0.556998 | 4.2:-38814310 | MS.gene28354:CDS |
| TACAGTGGTGATACGGAAGA+AGG | 0.559004 | 4.2:-38818195 | MS.gene28354:CDS |
| AATGCTGCAGGGAGCAGTAA+GGG | 0.560155 | 4.2:-38811380 | MS.gene28354:CDS |
| ACTTAAGCTGTGACTCAGGT+TGG | 0.561742 | 4.2:+38812131 | None:intergenic |
| GAACTTCTGATGATAGTCAC+TGG | 0.562651 | 4.2:-38811319 | MS.gene28354:CDS |
| CAGTTCTTTGAGTATTTGCA+AGG | 0.563576 | 4.2:-38811809 | MS.gene28354:intron |
| CTACTTCGAGAATTTAGTGA+GGG | 0.566132 | 4.2:-38811471 | MS.gene28354:CDS |
| AGTAAGGGGAGAGCTGGGAG+AGG | 0.566304 | 4.2:-38811365 | MS.gene28354:CDS |
| TGCTGTGGCCAGCTTACATG+TGG | 0.566414 | 4.2:-38816514 | MS.gene28354:intron |
| TCTTCCAGATAGTTGAGTGT+AGG | 0.573883 | 4.2:-38811895 | MS.gene28354:intron |
| GCATATTCACAACCAGGGGC+AGG | 0.573937 | 4.2:+38815667 | None:intergenic |
| CAGCAAAATTCAAATTTACA+GGG | 0.574826 | 4.2:+38814870 | None:intergenic |
| TCAGACCATGGTGAAAGAAC+TGG | 0.575256 | 4.2:-38814475 | MS.gene28354:CDS |
| GGGATTGGAATGGCTTCTTG+TGG | 0.575363 | 4.2:-38816463 | MS.gene28354:CDS |
| AGTGATTATGATGATGATGC+TGG | 0.577389 | 4.2:-38818156 | MS.gene28354:CDS |
| TCCTCGCTCTGGGATTGGAA+TGG | 0.579366 | 4.2:-38816473 | MS.gene28354:CDS |
| GTAGAAATCGTCATCTTCAA+GGG | 0.579796 | 4.2:+38818215 | None:intergenic |
| ACTGTGGCACTGTGGTAAAG+TGG | 0.587937 | 4.2:-38815452 | MS.gene28354:CDS |
| ACACCCATTATTATGAGCGG+TGG | 0.591402 | 4.2:-38814260 | MS.gene28354:CDS |
| TATAAGATCTACGAAGGTAA+CGG | 0.591928 | 4.2:+38816021 | None:intergenic |
| GAGCGAGTCCGAAAAGCGGT+TGG | 0.598853 | 4.2:-38816684 | MS.gene28354:CDS |
| CATCTCAACATCAAACATCC+AGG | 0.599803 | 4.2:+38816125 | None:intergenic |
| CCAATAGCAGCGCCACAAGT+GGG | 0.601504 | 4.2:+38816095 | None:intergenic |
| GTACTATTTACCTGAGCATG+AGG | 0.605750 | 4.2:-38811843 | MS.gene28354:CDS |
| ATGCTGCAGGGAGCAGTAAG+GGG | 0.605774 | 4.2:-38811379 | MS.gene28354:CDS |
| CACCCATTATTATGAGCGGT+GGG | 0.609091 | 4.2:-38814259 | MS.gene28354:CDS |
| TGAAATGGACGTATGCATAT+GGG | 0.610291 | 4.2:-38811865 | MS.gene28354:CDS |
| AGATGTCCTGAACCCACTTG+TGG | 0.620859 | 4.2:-38816107 | MS.gene28354:CDS |
| CAAAATTCAAATTTACAGGG+TGG | 0.621414 | 4.2:+38814873 | None:intergenic |
| TTATGAAGCAGCTAAGCAAG+AGG | 0.623330 | 4.2:-38814431 | MS.gene28354:CDS |
| CGAGGAGGCTCATCGTCCAG+TGG | 0.625879 | 4.2:-38815475 | MS.gene28354:CDS |
| ATCTGATGTAGATGCTAATG+GGG | 0.630234 | 4.2:-38811432 | MS.gene28354:CDS |
| TGATACGGAAGAAGGTGCGA+TGG | 0.632626 | 4.2:-38818187 | MS.gene28354:CDS |
| GAAGATGACGATTTCTACAG+TGG | 0.633360 | 4.2:-38818210 | MS.gene28354:CDS |
| TATGAAGCAGCTAAGCAAGA+GGG | 0.635282 | 4.2:-38814430 | MS.gene28354:CDS |
| TGAAGAGCGAGTCCGAAAAG+CGG | 0.637498 | 4.2:-38816688 | MS.gene28354:CDS |
| GATTGAGTCTCGTCGACCTG+AGG | 0.639074 | 4.2:-38818109 | MS.gene28354:intron |
| GCCATTCCAATCCCAGAGCG+AGG | 0.643551 | 4.2:+38816472 | None:intergenic |
| GACCATGGTGAAAGAACTGG+TGG | 0.643713 | 4.2:-38814472 | MS.gene28354:CDS |
| ACAGCTTAAGTTTATTACGG+AGG | 0.646409 | 4.2:-38812119 | MS.gene28354:CDS |
| CTTCGATATAAGATCTACGA+AGG | 0.648378 | 4.2:+38816015 | None:intergenic |
| GCTCATCGTCCAGTGGACTG+TGG | 0.650054 | 4.2:-38815468 | MS.gene28354:CDS |
| TACTATTTACCTGAGCATGA+GGG | 0.652156 | 4.2:-38811842 | MS.gene28354:CDS |
| ACTACTTCGAGAATTTAGTG+AGG | 0.655176 | 4.2:-38811472 | MS.gene28354:CDS |
| GTAACTGCATATTCACAACC+AGG | 0.655441 | 4.2:+38815661 | None:intergenic |
| AACAAAAGCTATACGAACAG+AGG | 0.655471 | 4.2:+38815602 | None:intergenic |
| AACTGCATATTCACAACCAG+GGG | 0.659172 | 4.2:+38815663 | None:intergenic |
| ATTGCAAATCTGGCATGTGG+TGG | 0.660392 | 4.2:+38811259 | None:intergenic |
| AACCACCAGTTCTTTCACCA+TGG | 0.660461 | 4.2:+38814470 | None:intergenic |
| TCAGTAAAGTGCATGATGCA+TGG | 0.665829 | 4.2:-38816719 | MS.gene28354:CDS |
| TAACTGCATATTCACAACCA+GGG | 0.669788 | 4.2:+38815662 | None:intergenic |
| TAGATTGGCCTTCAGCACTG+AGG | 0.685503 | 4.2:+38811639 | None:intergenic |
| GATACACCCATTATTATGAG+CGG | 0.686220 | 4.2:-38814263 | MS.gene28354:CDS |
| TTGATTGCAAATCTGGCATG+TGG | 0.691443 | 4.2:+38811256 | None:intergenic |
| GAATCTACAGTGCACCGAGG+AGG | 0.691639 | 4.2:-38815490 | MS.gene28354:intron |
| CCAGCATCCTAATACTAGCG+AGG | 0.692364 | 4.2:-38816640 | MS.gene28354:intron |
| CATGGTCTGACCATGCACCA+AGG | 0.699416 | 4.2:+38814488 | None:intergenic |
| CTTGGTGCATGGTCAGACCA+TGG | 0.701151 | 4.2:-38814487 | MS.gene28354:CDS |
| TCCAGTGGACTGTGGCACTG+TGG | 0.734400 | 4.2:-38815460 | MS.gene28354:CDS |
| GGCAGATTTGACATTCACAG+AGG | 0.749917 | 4.2:+38811280 | None:intergenic |
| TGTGAATCTACAGTGCACCG+AGG | 0.790165 | 4.2:-38815493 | MS.gene28354:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 38811247 | 38818280 | 38811247 | ID=MS.gene28354 |
| chr4.2 | mRNA | 38811247 | 38818280 | 38811247 | ID=MS.gene28354.t1;Parent=MS.gene28354 |
| chr4.2 | exon | 38818110 | 38818280 | 38818110 | ID=MS.gene28354.t1.exon1;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38818110 | 38818280 | 38818110 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38817260 | 38817384 | 38817260 | ID=MS.gene28354.t1.exon2;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38817260 | 38817384 | 38817260 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38816641 | 38816746 | 38816641 | ID=MS.gene28354.t1.exon3;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38816641 | 38816746 | 38816641 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38816434 | 38816524 | 38816434 | ID=MS.gene28354.t1.exon4;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38816434 | 38816524 | 38816434 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38816004 | 38816176 | 38816004 | ID=MS.gene28354.t1.exon5;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38816004 | 38816176 | 38816004 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38815597 | 38815701 | 38815597 | ID=MS.gene28354.t1.exon6;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38815597 | 38815701 | 38815597 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38815414 | 38815502 | 38815414 | ID=MS.gene28354.t1.exon7;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38815414 | 38815502 | 38815414 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38814870 | 38814977 | 38814870 | ID=MS.gene28354.t1.exon8;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38814870 | 38814977 | 38814870 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38814426 | 38814516 | 38814426 | ID=MS.gene28354.t1.exon9;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38814426 | 38814516 | 38814426 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38814245 | 38814334 | 38814245 | ID=MS.gene28354.t1.exon10;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38814245 | 38814334 | 38814245 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38812492 | 38812539 | 38812492 | ID=MS.gene28354.t1.exon11;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38812492 | 38812539 | 38812492 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38812108 | 38812179 | 38812108 | ID=MS.gene28354.t1.exon12;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38812108 | 38812179 | 38812108 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38811810 | 38811909 | 38811810 | ID=MS.gene28354.t1.exon13;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38811810 | 38811909 | 38811810 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38811596 | 38811722 | 38811596 | ID=MS.gene28354.t1.exon14;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38811596 | 38811722 | 38811596 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| chr4.2 | exon | 38811247 | 38811505 | 38811247 | ID=MS.gene28354.t1.exon15;Parent=MS.gene28354.t1 |
| chr4.2 | CDS | 38811247 | 38811505 | 38811247 | ID=cds.MS.gene28354.t1;Parent=MS.gene28354.t1 |
| Gene Sequence |
| Protein sequence |
