AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29751


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29751.t1 MTR_5g084570 96.000 50 2 0 1 50 1 50 1.07e-28 104
MS.gene29751.t1 MTR_5g084540 100.000 26 0 0 148 173 135 160 1.22e-14 68.2
MS.gene29751.t1 MTR_5g084530 74.000 50 12 1 1 50 1 49 3.07e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 83 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTACCTCTGATGCCAAAA+AGG 0.253882 5.3:-68389987 MS.gene29751:CDS
GTTGTAACCTCCACCATATT+TGG 0.288132 5.3:+68389810 None:intergenic
GAAGTAAACGATGCCAAATA+TGG 0.342429 5.3:-68389823 MS.gene29751:CDS
ACCTGCCATGGCAATGGTAT+TGG 0.432416 5.3:+68389512 None:intergenic
GAACCATAGCCAAGAGGCCT+AGG 0.443599 5.3:+68390050 None:intergenic
CATCCTAGGCCTCTTGGCTA+TGG 0.447886 5.3:-68390053 MS.gene29751:CDS
AATCCTCATCCTAGGCCTCT+TGG 0.458683 5.3:-68390059 MS.gene29751:CDS
GCCAATACCATTGCCATGGC+AGG 0.481585 5.3:-68389513 MS.gene29751:CDS
AGTGTTGTCCTTAGCTTGCA+TGG 0.498460 5.3:+68389465 None:intergenic
GGTGGTTACAACCATGGTGG+AGG 0.499176 5.3:-68389619 MS.gene29751:CDS
GGTGGCTACAACCATGGTGG+AGG 0.500815 5.3:-68389583 MS.gene29751:CDS
GGATACAACCACGGTGGTGG+TGG 0.504414 5.3:-68389721 MS.gene29751:CDS
GGTGGATACAACCACGGTGG+TGG 0.511490 5.3:-68389724 MS.gene29751:CDS
AGAAAAGCAATCCTCATCCT+AGG 0.516305 5.3:-68390067 MS.gene29751:CDS
GGATACAACAATGGTGGAGG+TGG 0.518813 5.3:-68389658 MS.gene29751:CDS
GGATACAACAATGGTGGAGG+TGG 0.518813 5.3:-68389700 MS.gene29751:CDS
GGAGGTTACAACCACGGCGG+TGG 0.523190 5.3:-68389799 MS.gene29751:CDS
GGCTACAACCATGGTGGAGG+CGG 0.523424 5.3:-68389580 MS.gene29751:CDS
GGCGGTTACAACCACGGTGG+AGG 0.523588 5.3:-68389541 MS.gene29751:CDS
GGCGGTTACAACCACGGTGG+AGG 0.523588 5.3:-68389562 MS.gene29751:CDS
GGATACAACCACGGTGGTGG+TGG 0.524452 5.3:-68389679 MS.gene29751:CDS
GGTTACAACCACGGTGGTGG+TGG 0.527779 5.3:-68389760 MS.gene29751:CDS
TAGCCAAGAGGCCTAGGATG+AGG 0.538496 5.3:+68390056 None:intergenic
ATTTGTTGCCATGCAAGCTA+AGG 0.539995 5.3:-68389473 MS.gene29751:CDS
AATGGTGGTGGCTACAACCA+TGG 0.544536 5.3:-68389589 MS.gene29751:CDS
GGTTACAACCACGGCGGTGG+TGG 0.544864 5.3:-68389796 MS.gene29751:CDS
GGAGGTGGTTACAATGGTGG+TGG 0.545650 5.3:-68389601 MS.gene29751:CDS
GGTTACAACCATGGTGGAGG+TGG 0.547395 5.3:-68389616 MS.gene29751:CDS
GGAGGTTACAACCACGGTGG+TGG 0.547932 5.3:-68389763 MS.gene29751:CDS
GGATACAACCACGGTGGTGG+TGG 0.553440 5.3:-68389637 MS.gene29751:CDS
TTCCTCAGAAGTCTCAGCTA+GGG 0.554700 5.3:-68390020 MS.gene29751:CDS
GGTGGATACAACCACGGTGG+TGG 0.558361 5.3:-68389640 MS.gene29751:CDS
GGTGGATACAACCACGGTGG+TGG 0.558361 5.3:-68389682 MS.gene29751:CDS
CATGTGAGCAGCACCTGCCA+TGG 0.559419 5.3:+68389500 None:intergenic
TTTCCTCAGAAGTCTCAGCT+AGG 0.560253 5.3:-68390021 MS.gene29751:CDS
CACGGCGGTGGTGGTTACAA+TGG 0.571191 5.3:-68389787 MS.gene29751:CDS
GGTTACAACCACGGTGGAGG+CGG 0.572554 5.3:-68389538 MS.gene29751:CDS
AACGATGCCAAATATGGTGG+AGG 0.573315 5.3:-68389817 MS.gene29751:CDS
GGTTACAACCACGGTGGAGG+CGG 0.574574 5.3:-68389559 MS.gene29751:CDS
GGTGGATACAACAATGGCGG+TGG 0.583166 5.3:-68389742 MS.gene29751:CDS
GGCGGTGGTGGTTACAATGG+TGG 0.594919 5.3:-68389784 MS.gene29751:CDS
GGTTGTAACCGCCTCCACCA+TGG 0.597627 5.3:+68389572 None:intergenic
CATGGTGGAGGTGGTTACAA+TGG 0.597788 5.3:-68389607 MS.gene29751:CDS
GGTGGATACAACAATGGTGG+AGG 0.598383 5.3:-68389661 MS.gene29751:CDS
GGTGGATACAACAATGGTGG+AGG 0.598383 5.3:-68389703 MS.gene29751:CDS
GGTGGTGGTTACAATGGTGG+AGG 0.609526 5.3:-68389781 MS.gene29751:CDS
GGTGGAGGTGGTTACAATGG+TGG 0.613370 5.3:-68389604 MS.gene29751:CDS
AGCAGCACCTGCCATGGCAA+TGG 0.620983 5.3:+68389506 None:intergenic
GGTGGTGGTGGTTACAACCA+TGG 0.640243 5.3:-68389625 MS.gene29751:CDS
GGTGGAGGCGGTTACAACCA+CGG 0.641005 5.3:-68389568 MS.gene29751:CDS
GGTTGCCAATACCATTGCCA+TGG 0.642022 5.3:-68389517 MS.gene29751:CDS
GTCCCTAGCTGAGACTTCTG+AGG 0.645915 5.3:+68390018 None:intergenic
CATTGTAACCACCACCGCCG+TGG 0.646466 5.3:+68389788 None:intergenic
GTAAACGATGCCAAATATGG+TGG 0.647144 5.3:-68389820 MS.gene29751:CDS
CATTGTAACCACCTCCACCA+TGG 0.649002 5.3:+68389608 None:intergenic
TAAGAAGAACCATAGCCAAG+AGG 0.664133 5.3:+68390044 None:intergenic
GGTGGTGGTGGATACAACAA+TGG 0.666044 5.3:-68389748 MS.gene29751:CDS
GGTGGAGGCGGTTACAACCA+CGG 0.674017 5.3:-68389547 MS.gene29751:CDS
GGTGGTGGATACAACAATGG+TGG 0.676280 5.3:-68389664 MS.gene29751:CDS
GGTGGTGGATACAACAATGG+TGG 0.676280 5.3:-68389706 MS.gene29751:CDS
ATTGGCAACCGCCTCCACCG+TGG 0.678280 5.3:+68389530 None:intergenic
GGTTGTAACCGCCTCCACCG+TGG 0.681332 5.3:+68389551 None:intergenic
GGTGGTGGTGGATACAACAA+TGG 0.684345 5.3:-68389667 MS.gene29751:CDS
GGTGGTGGTGGATACAACAA+TGG 0.684345 5.3:-68389709 MS.gene29751:CDS
TATGGTGGAGGTTACAACCA+CGG 0.702708 5.3:-68389805 MS.gene29751:CDS
AATGGTGGAGGTTACAACCA+CGG 0.710899 5.3:-68389769 MS.gene29751:CDS
TGTTGTATCCACCACCACCG+TGG 0.714982 5.3:+68389671 None:intergenic
TGTTGTATCCACCACCACCG+TGG 0.714982 5.3:+68389713 None:intergenic
TGTTGTATCCACCACCACCG+TGG 0.714982 5.3:+68389752 None:intergenic
GGTGGTGGCTACAACCATGG+TGG 0.723784 5.3:-68389586 MS.gene29751:CDS
GGTGGAGGTGGATACAACCA+CGG 0.728168 5.3:-68389646 MS.gene29751:CDS
GGTGGAGGTGGATACAACCA+CGG 0.728168 5.3:-68389688 MS.gene29751:CDS
GGTGGTGGTTACAACCATGG+TGG 0.730633 5.3:-68389622 MS.gene29751:CDS
AATGGCGGTGGATACAACCA+CGG 0.732761 5.3:-68389730 MS.gene29751:CDS
GGTGGTGGATACAACAATGG+CGG 0.734675 5.3:-68389745 MS.gene29751:CDS
GGTTGTAACCACCACCACCG+TGG 0.740537 5.3:+68389629 None:intergenic
GGCGGTGGATACAACCACGG+TGG 0.764343 5.3:-68389727 MS.gene29751:CDS
GGAGGCGGTTACAACCACGG+TGG 0.788813 5.3:-68389565 MS.gene29751:CDS
GGAGGCGGTTACAACCACGG+TGG 0.797498 5.3:-68389544 MS.gene29751:CDS
GGTGGAGGTTACAACCACGG+TGG 0.812517 5.3:-68389766 MS.gene29751:CDS
GGAGGTGGATACAACCACGG+TGG 0.823069 5.3:-68389643 MS.gene29751:CDS
GGAGGTGGATACAACCACGG+TGG 0.823069 5.3:-68389685 MS.gene29751:CDS
GGTGGAGGTTACAACCACGG+CGG 0.825418 5.3:-68389802 MS.gene29751:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATATATAGCTCACCCTTTT+TGG + chr5.3:68389562-68389581 None:intergenic 30.0%
GAAGTAAACGATGCCAAATA+TGG - chr5.3:68389709-68389728 MS.gene29751:CDS 35.0%
AACTACCTCTGATGCCAAAA+AGG - chr5.3:68389545-68389564 MS.gene29751:CDS 40.0%
ACTACCTCTGATGCCAAAAA+GGG - chr5.3:68389546-68389565 MS.gene29751:CDS 40.0%
AGAAAAGCAATCCTCATCCT+AGG - chr5.3:68389465-68389484 MS.gene29751:CDS 40.0%
GTAAACGATGCCAAATATGG+TGG - chr5.3:68389712-68389731 MS.gene29751:CDS 40.0%
GTTGTAACCTCCACCATATT+TGG + chr5.3:68389725-68389744 None:intergenic 40.0%
TAAGAAGAACCATAGCCAAG+AGG + chr5.3:68389491-68389510 None:intergenic 40.0%
! ATTTGTTGCCATGCAAGCTA+AGG - chr5.3:68390059-68390078 MS.gene29751:CDS 40.0%
AACGATGCCAAATATGGTGG+AGG - chr5.3:68389715-68389734 MS.gene29751:CDS 45.0%
TTCCTCAGAAGTCTCAGCTA+GGG - chr5.3:68389512-68389531 MS.gene29751:CDS 45.0%
TTTCCTCAGAAGTCTCAGCT+AGG - chr5.3:68389511-68389530 MS.gene29751:CDS 45.0%
! AATGGTGGAGGTTACAACCA+CGG - chr5.3:68389763-68389782 MS.gene29751:CDS 45.0%
! TATGGTGGAGGTTACAACCA+CGG - chr5.3:68389727-68389746 MS.gene29751:CDS 45.0%
!! AGTGTTGTCCTTAGCTTGCA+TGG + chr5.3:68390070-68390089 None:intergenic 45.0%
AATCCTCATCCTAGGCCTCT+TGG - chr5.3:68389473-68389492 MS.gene29751:CDS 50.0%
AATGGCGGTGGATACAACCA+CGG - chr5.3:68389802-68389821 MS.gene29751:CDS 50.0%
ACCTGCCATGGCAATGGTAT+TGG + chr5.3:68390023-68390042 None:intergenic 50.0%
CATTGTAACCACCTCCACCA+TGG + chr5.3:68389927-68389946 None:intergenic 50.0%
GGTGGATACAACAATGGTGG+AGG - chr5.3:68389829-68389848 MS.gene29751:CDS 50.0%
GGTTGCCAATACCATTGCCA+TGG - chr5.3:68390015-68390034 MS.gene29751:CDS 50.0%
! CATGGTGGAGGTGGTTACAA+TGG - chr5.3:68389925-68389944 MS.gene29751:intron 50.0%
! GGATACAACAATGGTGGAGG+TGG - chr5.3:68389832-68389851 MS.gene29751:CDS 50.0%
!! AATGGTGGTGGCTACAACCA+TGG - chr5.3:68389943-68389962 MS.gene29751:intron 50.0%
!! CTCACCCTTTTTGGCATCAG+AGG + chr5.3:68389553-68389572 None:intergenic 50.0%
!! GGTGGTGGATACAACAATGG+CGG - chr5.3:68389787-68389806 MS.gene29751:CDS 50.0%
!! GGTGGTGGATACAACAATGG+TGG - chr5.3:68389787-68389806 MS.gene29751:CDS 50.0%
!! GGTGGTGGTGGATACAACAA+TGG - chr5.3:68389784-68389803 MS.gene29751:CDS 50.0%
CATCCTAGGCCTCTTGGCTA+TGG - chr5.3:68389479-68389498 MS.gene29751:CDS 55.0%
GAACCATAGCCAAGAGGCCT+AGG + chr5.3:68389485-68389504 None:intergenic 55.0%
GCCAATACCATTGCCATGGC+AGG - chr5.3:68390019-68390038 MS.gene29751:CDS 55.0%
GGAGGTGGTTACAATGGTGG+TGG - chr5.3:68389931-68389950 MS.gene29751:intron 55.0%
GGTGGAGGTGGATACAACCA+CGG - chr5.3:68389844-68389863 MS.gene29751:CDS 55.0%
GGTGGAGGTGGTTACAATGG+TGG - chr5.3:68389928-68389947 MS.gene29751:intron 55.0%
GGTGGATACAACAATGGCGG+TGG - chr5.3:68389790-68389809 MS.gene29751:CDS 55.0%
GGTGGTTACAACCATGGTGG+AGG - chr5.3:68389913-68389932 MS.gene29751:intron 55.0%
GTCCCTAGCTGAGACTTCTG+AGG + chr5.3:68389517-68389536 None:intergenic 55.0%
TAGCCAAGAGGCCTAGGATG+AGG + chr5.3:68389479-68389498 None:intergenic 55.0%
TGTTGTATCCACCACCACCG+TGG + chr5.3:68389783-68389802 None:intergenic 55.0%
! GGTGGTGGTTACAACCATGG+TGG - chr5.3:68389910-68389929 MS.gene29751:intron 55.0%
! GGTGGTGGTTACAATGGTGG+AGG - chr5.3:68389751-68389770 MS.gene29751:CDS 55.0%
! GGTTACAACCATGGTGGAGG+TGG - chr5.3:68389916-68389935 MS.gene29751:intron 55.0%
!! GGTGGTGGTGGTTACAACCA+TGG - chr5.3:68389907-68389926 MS.gene29751:intron 55.0%
AGCAGCACCTGCCATGGCAA+TGG + chr5.3:68390029-68390048 None:intergenic 60.0%
CATGTGAGCAGCACCTGCCA+TGG + chr5.3:68390035-68390054 None:intergenic 60.0%
CATTGTAACCACCACCGCCG+TGG + chr5.3:68389747-68389766 None:intergenic 60.0%
GGAGGTGGATACAACCACGG+TGG - chr5.3:68389847-68389866 MS.gene29751:intron 60.0%
GGAGGTTACAACCACGGTGG+TGG - chr5.3:68389769-68389788 MS.gene29751:CDS 60.0%
GGTGGAGGCGGTTACAACCA+CGG - chr5.3:68389964-68389983 MS.gene29751:intron 60.0%
GGTGGAGGTTACAACCACGG+CGG - chr5.3:68389730-68389749 MS.gene29751:CDS 60.0%
GGTGGAGGTTACAACCACGG+TGG - chr5.3:68389730-68389749 MS.gene29751:CDS 60.0%
GGTGGATACAACCACGGTGG+TGG - chr5.3:68389808-68389827 MS.gene29751:CDS 60.0%
GGTGGCTACAACCATGGTGG+AGG - chr5.3:68389949-68389968 MS.gene29751:intron 60.0%
GGTTACAACCACGGTGGAGG+CGG - chr5.3:68389973-68389992 MS.gene29751:intron 60.0%
GGTTGTAACCACCACCACCG+TGG + chr5.3:68389906-68389925 None:intergenic 60.0%
GGTTGTAACCGCCTCCACCA+TGG + chr5.3:68389963-68389982 None:intergenic 60.0%
! CACGGCGGTGGTGGTTACAA+TGG - chr5.3:68389745-68389764 MS.gene29751:CDS 60.0%
! GGATACAACCACGGTGGTGG+TGG - chr5.3:68389811-68389830 MS.gene29751:CDS 60.0%
! GGCGGTGGTGGTTACAATGG+TGG - chr5.3:68389748-68389767 MS.gene29751:CDS 60.0%
! GGCTACAACCATGGTGGAGG+CGG - chr5.3:68389952-68389971 MS.gene29751:intron 60.0%
! GGTGGTGGCTACAACCATGG+TGG - chr5.3:68389946-68389965 MS.gene29751:intron 60.0%
! GGTTACAACCACGGTGGTGG+TGG - chr5.3:68389772-68389791 MS.gene29751:CDS 60.0%
ATTGGCAACCGCCTCCACCG+TGG + chr5.3:68390005-68390024 None:intergenic 65.0%
GGAGGCGGTTACAACCACGG+TGG - chr5.3:68389967-68389986 MS.gene29751:intron 65.0%
GGAGGTTACAACCACGGCGG+TGG - chr5.3:68389733-68389752 MS.gene29751:CDS 65.0%
GGCGGTGGATACAACCACGG+TGG - chr5.3:68389805-68389824 MS.gene29751:CDS 65.0%
GGCGGTTACAACCACGGTGG+AGG - chr5.3:68389970-68389989 MS.gene29751:intron 65.0%
GGTTACAACCACGGCGGTGG+TGG - chr5.3:68389736-68389755 MS.gene29751:CDS 65.0%
GGTTGTAACCGCCTCCACCG+TGG + chr5.3:68389984-68390003 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 68389456 68390098 68389456 ID=MS.gene29751
chr5.3 mRNA 68389456 68390098 68389456 ID=MS.gene29751.t1;Parent=MS.gene29751
chr5.3 exon 68389987 68390098 68389987 ID=MS.gene29751.t1.exon1;Parent=MS.gene29751.t1
chr5.3 CDS 68389987 68390098 68389987 ID=cds.MS.gene29751.t1;Parent=MS.gene29751.t1
chr5.3 exon 68389456 68389865 68389456 ID=MS.gene29751.t1.exon2;Parent=MS.gene29751.t1
chr5.3 CDS 68389456 68389865 68389456 ID=cds.MS.gene29751.t1;Parent=MS.gene29751.t1
Gene Sequence

>MS.gene29751

ATGGATTCGAGAAAAGCAATCCTCATCCTAGGCCTCTTGGCTATGGTTCTTCTTATTTCCTCAGAAGTCTCAGCTAGGGACTTAACTGAAACTACCTCTGATGCCAAAAAGGGTGAGCTATATATTATTTTTCTTTATAAATACTTTCACGAGCATTTTCTATATTTTTTAAATTCTTTTCTAGTTAATTATATATAAAGTAATGATTCATATTATATGTGCTGTGTTTGCAGAAGTTGTTGAAAAGACTAATGAAGTAAACGATGCCAAATATGGTGGAGGTTACAACCACGGCGGTGGTGGTTACAATGGTGGAGGTTACAACCACGGTGGTGGTGGATACAACAATGGCGGTGGATACAACCACGGTGGTGGTGGATACAACAATGGTGGAGGTGGATACAACCACGGTGGTGGTGGATACAACAATGGTGGAGGTGGATACAACCACGGTGGTGGTGGTTACAACCATGGTGGAGGTGGTTACAATGGTGGTGGCTACAACCATGGTGGAGGCGGTTACAACCACGGTGGAGGCGGTTACAACCACGGTGGAGGCGGTTGCCAATACCATTGCCATGGCAGGTGCTGCTCACATGCTGAATTTGTTGCCATGCAAGCTAAGGACAACACTCAGAATTGA

Protein sequence

>MS.gene29751.t1

MDSRKAILILGLLAMVLLISSEVSARDLTETTSDAKKEVVEKTNEVNDAKYGGGYNHGGGGYNGGGYNHGGGGYNNGGGYNHGGGGYNNGGGGYNHGGGGYNNGGGGYNHGGGGYNHGGGGYNGGGYNHGGGGYNHGGGGYNHGGGGCQYHCHGRCCSHAEFVAMQAKDNTQN