Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30073.t1 | XP_024633451.1 | 84.8 | 46 | 5 | 1 | 1 | 44 | 26 | 71 | 4.60E-09 | 70.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30073.t1 | G7L456 | 48.8 | 84 | 29 | 2 | 1 | 70 | 1 | 84 | 9.9e-06 | 59.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene30073 | 0.830735 | 2.48E-55 | -1.69E-46 |
| MS.gene049587 | MS.gene30073 | 0.812463 | 4.19E-51 | -1.69E-46 |
| MS.gene049853 | MS.gene30073 | 0.856449 | 3.19E-62 | -1.69E-46 |
| MS.gene049968 | MS.gene30073 | 0.806333 | 8.66E-50 | -1.69E-46 |
| MS.gene051932 | MS.gene30073 | 0.808445 | 3.09E-50 | -1.69E-46 |
| MS.gene052447 | MS.gene30073 | 0.821692 | 3.53E-53 | -1.69E-46 |
| MS.gene053512 | MS.gene30073 | 0.803665 | 3.13E-49 | -1.69E-46 |
| MS.gene053974 | MS.gene30073 | 0.801605 | 8.32E-49 | -1.69E-46 |
| MS.gene054174 | MS.gene30073 | 0.84929 | 3.56E-60 | -1.69E-46 |
| MS.gene054175 | MS.gene30073 | 0.854173 | 1.47E-61 | -1.69E-46 |
| MS.gene05435 | MS.gene30073 | 0.810661 | 1.03E-50 | -1.69E-46 |
| MS.gene054484 | MS.gene30073 | 0.859392 | 4.27E-63 | -1.69E-46 |
| MS.gene055017 | MS.gene30073 | 0.830098 | 3.56E-55 | -1.69E-46 |
| MS.gene055056 | MS.gene30073 | 0.826538 | 2.57E-54 | -1.69E-46 |
| MS.gene055058 | MS.gene30073 | 0.817818 | 2.71E-52 | -1.69E-46 |
| MS.gene055952 | MS.gene30073 | 0.824652 | 7.19E-54 | -1.69E-46 |
| MS.gene05762 | MS.gene30073 | 0.823219 | 1.56E-53 | -1.69E-46 |
| MS.gene058515 | MS.gene30073 | 0.81931 | 1.24E-52 | -1.69E-46 |
| MS.gene059276 | MS.gene30073 | 0.840506 | 8.34E-58 | -1.69E-46 |
| MS.gene059458 | MS.gene30073 | 0.824826 | 6.54E-54 | -1.69E-46 |
| MS.gene059459 | MS.gene30073 | 0.814724 | 1.33E-51 | -1.69E-46 |
| MS.gene059460 | MS.gene30073 | 0.802314 | 5.95E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene30073 | 0.806028 | 1.00E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30073.t1 | MTR_7g012300 | 57.500 | 120 | 36 | 4 | 1 | 105 | 1 | 120 | 2.13e-27 | 98.6 |
| MS.gene30073.t1 | MTR_2g043940 | 72.414 | 58 | 16 | 0 | 59 | 116 | 305 | 362 | 9.51e-17 | 74.7 |
| MS.gene30073.t1 | MTR_7g056577 | 66.667 | 66 | 17 | 1 | 56 | 116 | 47 | 112 | 3.37e-16 | 69.7 |
| MS.gene30073.t1 | MTR_5g061300 | 54.545 | 99 | 37 | 3 | 21 | 112 | 229 | 326 | 8.79e-16 | 71.6 |
| MS.gene30073.t1 | MTR_5g061660 | 60.000 | 75 | 16 | 1 | 56 | 116 | 71 | 145 | 1.09e-13 | 63.9 |
| MS.gene30073.t1 | MTR_0039s0180 | 63.934 | 61 | 18 | 1 | 57 | 113 | 58 | 118 | 1.79e-13 | 62.4 |
| MS.gene30073.t1 | MTR_2g461540 | 60.000 | 75 | 16 | 2 | 56 | 116 | 3 | 77 | 1.10e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAACCATTTCTTGAAGTT+GGG | 0.352117 | 7.1:+63651399 | None:intergenic |
| TTTCAACCATTTCTTGAAGT+TGG | 0.356872 | 7.1:+63651398 | None:intergenic |
| CTAGTCCCAACTTCAAGAAA+TGG | 0.372385 | 7.1:-63651404 | MS.gene30073:intron |
| AAGCAATTGGATGTAGAAAC+TGG | 0.389575 | 7.1:-63651532 | MS.gene30073:CDS |
| ATGGGAAAGGAGTGTGTACC+AGG | 0.471236 | 7.1:-63651340 | MS.gene30073:intron |
| CTTGAGGACAACATAGGTGA+TGG | 0.475620 | 7.1:-63650891 | MS.gene30073:CDS |
| ACTTCAAGAAATGGTTGAAA+AGG | 0.483448 | 7.1:-63651395 | MS.gene30073:CDS |
| AGGAAGTAGAGGAAAGCAAT+TGG | 0.498254 | 7.1:-63651545 | MS.gene30073:CDS |
| ATGAAATTCAAGAGGATGAT+CGG | 0.537624 | 7.1:-63650818 | MS.gene30073:CDS |
| GAATTTCTTGAGGACAACAT+AGG | 0.539954 | 7.1:-63650897 | MS.gene30073:CDS |
| TCAAGAAATGGTTGAAAAGG+AGG | 0.541538 | 7.1:-63651392 | MS.gene30073:CDS |
| TTGAGGACAACATAGGTGAT+GGG | 0.545907 | 7.1:-63650890 | MS.gene30073:CDS |
| CGCATATCATGAATTTCTTG+AGG | 0.553600 | 7.1:-63650907 | MS.gene30073:CDS |
| GAAACTCGACAAGGAAGTAG+AGG | 0.581711 | 7.1:-63651556 | MS.gene30073:CDS |
| TATATATGATGAATTTCAAG+AGG | 0.613332 | 7.1:-63650853 | MS.gene30073:CDS |
| TCTAGATGATGAAATTCAAG+AGG | 0.613572 | 7.1:-63650826 | MS.gene30073:CDS |
| CATAGGTGATGGGTTTCAGA+AGG | 0.615780 | 7.1:-63650880 | MS.gene30073:CDS |
| CATAGATGAGGAATTTCAAG+AGG | 0.630357 | 7.1:-63650934 | MS.gene30073:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATGTAAAAATTTAAAGTTA+CGG | - | chr7.1:63650882-63650901 | MS.gene30073:CDS | 10.0% |
| !! | AAATTGTGTTTAACTAATAT+AGG | - | chr7.1:63650906-63650925 | MS.gene30073:CDS | 15.0% |
| !! | AAGTTGAAGAAACTTAAAAA+TGG | - | chr7.1:63651160-63651179 | MS.gene30073:intron | 20.0% |
| !! | AGTTGAAGAAACTTAAAAAT+GGG | - | chr7.1:63651161-63651180 | MS.gene30073:intron | 20.0% |
| !! | TATATATGATGAATTTCAAG+AGG | - | chr7.1:63651527-63651546 | MS.gene30073:intron | 20.0% |
| !!! | AATTATTTTCATATGCAACA+AGG | - | chr7.1:63651396-63651415 | MS.gene30073:CDS | 20.0% |
| !!! | TTTTATTTTCATATGCAACA+AGG | - | chr7.1:63651354-63651373 | MS.gene30073:CDS | 20.0% |
| ! | CTTTGAATCAACAATATCTA+CGG | - | chr7.1:63651318-63651337 | MS.gene30073:intron | 25.0% |
| ! | TGTAATGATAGAATTAGGAA+GGG | - | chr7.1:63651186-63651205 | MS.gene30073:intron | 25.0% |
| ! | TTGTAATGATAGAATTAGGA+AGG | - | chr7.1:63651185-63651204 | MS.gene30073:intron | 25.0% |
| ! | TTGTGTTTAACTAATATAGG+CGG | - | chr7.1:63650909-63650928 | MS.gene30073:CDS | 25.0% |
| ACTTCAAGAAATGGTTGAAA+AGG | - | chr7.1:63650985-63651004 | MS.gene30073:CDS | 30.0% | |
| ATGAAATTCAAGAGGATGAT+CGG | - | chr7.1:63651562-63651581 | MS.gene30073:CDS | 30.0% | |
| CAAAACAAATGTTCTACTTG+CGG | - | chr7.1:63651216-63651235 | MS.gene30073:intron | 30.0% | |
| TCTAGATGATGAAATTCAAG+AGG | - | chr7.1:63651554-63651573 | MS.gene30073:CDS | 30.0% | |
| TGAAAAAGATGACATAGATG+AGG | - | chr7.1:63651434-63651453 | MS.gene30073:intron | 30.0% | |
| TTCAACCATTTCTTGAAGTT+GGG | + | chr7.1:63650984-63651003 | None:intergenic | 30.0% | |
| TTTCAACCATTTCTTGAAGT+TGG | + | chr7.1:63650985-63651004 | None:intergenic | 30.0% | |
| !! | CGTAGATATTGTTGATTCAA+AGG | + | chr7.1:63651320-63651339 | None:intergenic | 30.0% |
| !!! | ATGTTTTTTGAAACTCGACA+AGG | - | chr7.1:63650815-63650834 | MS.gene30073:CDS | 30.0% |
| !!! | ATTTGGTTTTTATTTGTGCG+AGG | - | chr7.1:63651267-63651286 | MS.gene30073:intron | 30.0% |
| !!! | GAAGCTTTTAAAGCTGTTAT+GGG | - | chr7.1:63651022-63651041 | MS.gene30073:intron | 30.0% |
| !!! | TTTTAAAGCTGTTATGGGAA+AGG | - | chr7.1:63651027-63651046 | MS.gene30073:intron | 30.0% |
| AAGCAATTGGATGTAGAAAC+TGG | - | chr7.1:63650848-63650867 | MS.gene30073:CDS | 35.0% | |
| CATAGATGAGGAATTTCAAG+AGG | - | chr7.1:63651446-63651465 | MS.gene30073:intron | 35.0% | |
| CGCATATCATGAATTTCTTG+AGG | - | chr7.1:63651473-63651492 | MS.gene30073:intron | 35.0% | |
| GAATTTCTTGAGGACAACAT+AGG | - | chr7.1:63651483-63651502 | MS.gene30073:intron | 35.0% | |
| GGGCTTGTAATGATAGAATT+AGG | - | chr7.1:63651181-63651200 | MS.gene30073:intron | 35.0% | |
| TCAAGAAATGGTTGAAAAGG+AGG | - | chr7.1:63650988-63651007 | MS.gene30073:CDS | 35.0% | |
| !! | GCGTGTTTTACTTGTTGATT+TGG | - | chr7.1:63651250-63651269 | MS.gene30073:intron | 35.0% |
| !!! | CGAAGCTTTTAAAGCTGTTA+TGG | - | chr7.1:63651021-63651040 | MS.gene30073:intron | 35.0% |
| AACACAATCAAAATCCGACC+TGG | + | chr7.1:63651061-63651080 | None:intergenic | 40.0% | |
| AGGAAGTAGAGGAAAGCAAT+TGG | - | chr7.1:63650835-63650854 | MS.gene30073:CDS | 40.0% | |
| CTAGTCCCAACTTCAAGAAA+TGG | - | chr7.1:63650976-63650995 | MS.gene30073:CDS | 40.0% | |
| TTGAGGACAACATAGGTGAT+GGG | - | chr7.1:63651490-63651509 | MS.gene30073:intron | 40.0% | |
| CTTGAGGACAACATAGGTGA+TGG | - | chr7.1:63651489-63651508 | MS.gene30073:intron | 45.0% | |
| GAAACTCGACAAGGAAGTAG+AGG | - | chr7.1:63650824-63650843 | MS.gene30073:CDS | 45.0% | |
| !! | CATAGGTGATGGGTTTCAGA+AGG | - | chr7.1:63651500-63651519 | MS.gene30073:intron | 45.0% |
| ! | ATGGGAAAGGAGTGTGTACC+AGG | - | chr7.1:63651040-63651059 | MS.gene30073:intron | 50.0% |
| ! | GAAAGGAGTGTGTACCAGGT+CGG | - | chr7.1:63651044-63651063 | MS.gene30073:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 63650815 | 63651587 | 63650815 | ID=MS.gene30073 |
| chr7.1 | mRNA | 63650815 | 63651587 | 63650815 | ID=MS.gene30073.t1;Parent=MS.gene30073 |
| chr7.1 | exon | 63651528 | 63651587 | 63651528 | ID=MS.gene30073.t1.exon1;Parent=MS.gene30073.t1 |
| chr7.1 | CDS | 63651528 | 63651587 | 63651528 | ID=cds.MS.gene30073.t1;Parent=MS.gene30073.t1 |
| chr7.1 | exon | 63651341 | 63651422 | 63651341 | ID=MS.gene30073.t1.exon2;Parent=MS.gene30073.t1 |
| chr7.1 | CDS | 63651341 | 63651422 | 63651341 | ID=cds.MS.gene30073.t1;Parent=MS.gene30073.t1 |
| chr7.1 | exon | 63650815 | 63651026 | 63650815 | ID=MS.gene30073.t1.exon3;Parent=MS.gene30073.t1 |
| chr7.1 | CDS | 63650815 | 63651026 | 63650815 | ID=cds.MS.gene30073.t1;Parent=MS.gene30073.t1 |
| Gene Sequence |
| Protein sequence |
