Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31666.t1 | XP_003588460.1 | 98.7 | 232 | 3 | 0 | 76 | 307 | 1 | 232 | 2.30E-124 | 455.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31666.t1 | P36525 | 30.9 | 149 | 96 | 3 | 81 | 227 | 30 | 173 | 2.2e-11 | 71.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31666.t1 | G7I293 | 98.7 | 232 | 3 | 0 | 76 | 307 | 1 | 232 | 1.6e-124 | 455.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049821 | MS.gene31666 | 0.82324 | 1.54E-53 | -1.69E-46 |
| MS.gene050237 | MS.gene31666 | 0.809306 | 2.02E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene31666 | 0.816943 | 4.26E-52 | -1.69E-46 |
| MS.gene050719 | MS.gene31666 | 0.806878 | 6.64E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene31666 | 0.808602 | 2.86E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene31666 | 0.80357 | 3.27E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene31666 | 0.808329 | 3.27E-50 | -1.69E-46 |
| MS.gene052133 | MS.gene31666 | 0.803594 | 3.24E-49 | -1.69E-46 |
| MS.gene052773 | MS.gene31666 | 0.801886 | 7.29E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene31666 | 0.803844 | 2.87E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene31666 | 0.805198 | 1.50E-49 | -1.69E-46 |
| MS.gene053085 | MS.gene31666 | 0.804916 | 1.72E-49 | -1.69E-46 |
| MS.gene053216 | MS.gene31666 | 0.822844 | 1.91E-53 | -1.69E-46 |
| MS.gene053630 | MS.gene31666 | 0.821029 | 5.02E-53 | -1.69E-46 |
| MS.gene05415 | MS.gene31666 | 0.803063 | 4.17E-49 | -1.69E-46 |
| MS.gene054472 | MS.gene31666 | 0.801864 | 7.36E-49 | -1.69E-46 |
| MS.gene05462 | MS.gene31666 | 0.82296 | 1.79E-53 | -1.69E-46 |
| MS.gene055465 | MS.gene31666 | 0.807917 | 4.00E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene31666 | 0.814455 | 1.53E-51 | -1.69E-46 |
| MS.gene055468 | MS.gene31666 | 0.8165 | 5.36E-52 | -1.69E-46 |
| MS.gene055608 | MS.gene31666 | 0.810835 | 9.46E-51 | -1.69E-46 |
| MS.gene055711 | MS.gene31666 | 0.814187 | 1.75E-51 | -1.69E-46 |
| MS.gene055778 | MS.gene31666 | 0.803712 | 3.06E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene31666 | 0.81217 | 4.85E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene31666 | 0.802261 | 6.10E-49 | -1.69E-46 |
| MS.gene056760 | MS.gene31666 | 0.817184 | 3.76E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene31666 | 0.816908 | 4.34E-52 | -1.69E-46 |
| MS.gene057314 | MS.gene31666 | 0.813945 | 1.98E-51 | -1.69E-46 |
| MS.gene057693 | MS.gene31666 | 0.812347 | 4.44E-51 | -1.69E-46 |
| MS.gene057869 | MS.gene31666 | 0.801868 | 7.35E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene31666 | 0.823834 | 1.12E-53 | -1.69E-46 |
| MS.gene058636 | MS.gene31666 | 0.81857 | 1.83E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene31666 | 0.803225 | 3.86E-49 | -1.69E-46 |
| MS.gene059220 | MS.gene31666 | 0.800453 | 1.43E-48 | -1.69E-46 |
| MS.gene059625 | MS.gene31666 | 0.804362 | 2.24E-49 | -1.69E-46 |
| MS.gene059725 | MS.gene31666 | 0.805122 | 1.55E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene31666 | 0.806267 | 8.94E-50 | -1.69E-46 |
| MS.gene060553 | MS.gene31666 | 0.803411 | 3.53E-49 | -1.69E-46 |
| MS.gene060587 | MS.gene31666 | 0.812722 | 3.67E-51 | -1.69E-46 |
| MS.gene060634 | MS.gene31666 | -0.810574 | 1.08E-50 | -1.69E-46 |
| MS.gene060918 | MS.gene31666 | 0.827528 | 1.49E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31666.t1 | MTR_1g007480 | 98.707 | 232 | 3 | 0 | 76 | 307 | 1 | 232 | 9.17e-170 | 470 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31666.t1 | AT4G31460 | 66.497 | 197 | 66 | 0 | 76 | 272 | 1 | 197 | 2.61e-95 | 281 |
Find 59 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCCATAACAACTTTGCTTT+TGG | 0.225318 | 1.1:+12035858 | None:intergenic |
| TCCCAAAAGCAAAGTTGTTA+TGG | 0.322622 | 1.1:-12035860 | MS.gene31666:CDS |
| CAAAAGATGGACACAGAAAT+AGG | 0.324056 | 1.1:-12031414 | MS.gene31666:CDS |
| TTGTGAAGTGATAGGGAAAA+TGG | 0.333186 | 1.1:-12035977 | MS.gene31666:CDS |
| GCTGGGAAACATATTCAGTT+TGG | 0.336618 | 1.1:-12035814 | MS.gene31666:CDS |
| CTGGGAAACATATTCAGTTT+GGG | 0.362976 | 1.1:-12035813 | MS.gene31666:CDS |
| CCCAAAAGCAAAGTTGTTAT+GGG | 0.364570 | 1.1:-12035859 | MS.gene31666:CDS |
| GATGAAGAAAGTGCTGAAAA+AGG | 0.397823 | 1.1:-12035935 | MS.gene31666:CDS |
| TTAGTGAAGATGGTGGTAAC+AGG | 0.399831 | 1.1:-12035783 | MS.gene31666:intron |
| CCAATTGGAATTGTTCCAAT+TGG | 0.412253 | 1.1:+12038277 | None:intergenic |
| GGATGAAGCGAAGTTTGAAC+AGG | 0.428127 | 1.1:-12031316 | MS.gene31666:CDS |
| AGCCTCTTATCTTGTACGTT+AGG | 0.429435 | 1.1:+12031546 | None:intergenic |
| TCTCCAACTTTGAGCCTGTC+AGG | 0.432513 | 1.1:+12031102 | None:intergenic |
| TCAAACTTCGCTTCATCCTC+CGG | 0.436492 | 1.1:+12031321 | None:intergenic |
| CCAATTGGAACAATTCCAAT+TGG | 0.436581 | 1.1:-12038277 | MS.gene31666:CDS |
| GGGAATAGTGTTAGTGAAGA+TGG | 0.437529 | 1.1:-12035793 | MS.gene31666:CDS |
| TGTATGCTTTCATTACCAAT+TGG | 0.442824 | 1.1:-12038292 | MS.gene31666:CDS |
| AGATTTGAGATTAGCTGAAA+GGG | 0.447839 | 1.1:-12031286 | MS.gene31666:CDS |
| ATTCCAATTGGTGTGTCTAC+GGG | 0.449805 | 1.1:-12038265 | MS.gene31666:CDS |
| AATTCCAATTGGTGTGTCTA+CGG | 0.452882 | 1.1:-12038266 | MS.gene31666:CDS |
| GAGAAGTTGTATGAAGAGCT+TGG | 0.456277 | 1.1:-12031369 | MS.gene31666:CDS |
| AACCTAACGTACAAGATAAG+AGG | 0.473606 | 1.1:-12031548 | MS.gene31666:CDS |
| CGAAGGAATCGATAGAGAAA+TGG | 0.474673 | 1.1:-12035885 | MS.gene31666:CDS |
| ATAGGGAAAATGGCGTTTAG+AGG | 0.475804 | 1.1:-12035967 | MS.gene31666:CDS |
| AAGATTTGAGATTAGCTGAA+AGG | 0.477923 | 1.1:-12031287 | MS.gene31666:CDS |
| TAATACGAATGTGACGATCT+AGG | 0.481354 | 1.1:+12031511 | None:intergenic |
| GAAGACTCCTTATCAAAAGA+TGG | 0.484789 | 1.1:-12031427 | MS.gene31666:CDS |
| TAGTTTGTGCTTGTTCATGC+TGG | 0.485930 | 1.1:+12031230 | None:intergenic |
| TTCCCCGTAGACACACCAAT+TGG | 0.494900 | 1.1:+12038262 | None:intergenic |
| ATAGATAGAAGCATGACCTT+TGG | 0.505536 | 1.1:-12038322 | None:intergenic |
| CTGATAGGACAAGGAGAACA+TGG | 0.516494 | 1.1:-12031572 | MS.gene31666:intron |
| CGAGGGAGCTGCTAAAATTG+AGG | 0.519910 | 1.1:-12031181 | MS.gene31666:CDS |
| CTTCGTTGCATCGACAAGGC+AGG | 0.525155 | 1.1:-12031471 | MS.gene31666:CDS |
| CAATGGAGTGGAAATTGCGT+TGG | 0.526744 | 1.1:+12036071 | None:intergenic |
| GAGGGAGCTGCTAAAATTGA+GGG | 0.526843 | 1.1:-12031180 | MS.gene31666:CDS |
| GAACAAGCACAAACTAATTG+AGG | 0.531079 | 1.1:-12031223 | MS.gene31666:CDS |
| ATTGAGGTAGAAAGTAAAGA+TGG | 0.533016 | 1.1:-12031207 | MS.gene31666:CDS |
| ACTCGATTCATCAAAGCGAT+TGG | 0.536622 | 1.1:-12031148 | MS.gene31666:CDS |
| TGAAGAGCTTGGAAAGAAAG+AGG | 0.537302 | 1.1:-12031358 | MS.gene31666:CDS |
| GCTCCTGACAGGCTCAAAGT+TGG | 0.542014 | 1.1:-12031105 | MS.gene31666:CDS |
| CGTATGTGTTAGCTCCTGAC+AGG | 0.548037 | 1.1:-12031116 | MS.gene31666:CDS |
| CGCCCTTCGTTGCATCGACA+AGG | 0.548075 | 1.1:-12031475 | MS.gene31666:CDS |
| GGAGCTAACACATACGAGAC+AGG | 0.555598 | 1.1:+12031123 | None:intergenic |
| TGCCTTGTCGATGCAACGAA+GGG | 0.567208 | 1.1:+12031473 | None:intergenic |
| CTGCCTTGTCGATGCAACGA+AGG | 0.571094 | 1.1:+12031472 | None:intergenic |
| TCTATCGATTCCTTCGCTCG+TGG | 0.572095 | 1.1:+12035892 | None:intergenic |
| AGAGATCTGCTACGACGCAA+TGG | 0.580771 | 1.1:+12036054 | None:intergenic |
| AGAAAGTAAAGATGGTGACG+AGG | 0.602281 | 1.1:-12031199 | MS.gene31666:CDS |
| TTCCAATTGGTGTGTCTACG+GGG | 0.625760 | 1.1:-12038264 | MS.gene31666:CDS |
| GAATATGACTCCACGAGCGA+AGG | 0.626713 | 1.1:-12035902 | MS.gene31666:CDS |
| TCTGCTACGACGCAATGGAG+TGG | 0.629742 | 1.1:+12036059 | None:intergenic |
| GATGCAACGAAGGGCGTGAG+TGG | 0.648741 | 1.1:+12031482 | None:intergenic |
| TTCTCAAACGAGGAACAAAG+AGG | 0.656436 | 1.1:-12038227 | MS.gene31666:intron |
| CGTTGCATCGACAAGGCAGG+CGG | 0.656785 | 1.1:-12031468 | MS.gene31666:CDS |
| GTTATGGGTCGTGCAAAACG+TGG | 0.672525 | 1.1:-12035844 | MS.gene31666:CDS |
| AATAGTGTTAGTGAAGATGG+TGG | 0.675297 | 1.1:-12035790 | MS.gene31666:CDS |
| GAAAGTAAAGATGGTGACGA+GGG | 0.680500 | 1.1:-12031198 | MS.gene31666:CDS |
| AAGCATACATACGATGCCAA+AGG | 0.681385 | 1.1:+12038306 | None:intergenic |
| GATGAAGCGAAGTTTGAACA+GGG | 0.683364 | 1.1:-12031315 | MS.gene31666:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 12031086 | 12038332 | 12031086 | ID=MS.gene31666 |
| chr1.1 | mRNA | 12031086 | 12038332 | 12031086 | ID=MS.gene31666.t1;Parent=MS.gene31666 |
| chr1.1 | exon | 12038228 | 12038332 | 12038228 | ID=MS.gene31666.t1.exon1;Parent=MS.gene31666.t1 |
| chr1.1 | CDS | 12038228 | 12038332 | 12038228 | ID=cds.MS.gene31666.t1;Parent=MS.gene31666.t1 |
| chr1.1 | exon | 12035784 | 12036100 | 12035784 | ID=MS.gene31666.t1.exon2;Parent=MS.gene31666.t1 |
| chr1.1 | CDS | 12035784 | 12036100 | 12035784 | ID=cds.MS.gene31666.t1;Parent=MS.gene31666.t1 |
| chr1.1 | exon | 12031086 | 12031587 | 12031086 | ID=MS.gene31666.t1.exon3;Parent=MS.gene31666.t1 |
| chr1.1 | CDS | 12031086 | 12031587 | 12031086 | ID=cds.MS.gene31666.t1;Parent=MS.gene31666.t1 |
| Gene Sequence |
| Protein sequence |
