AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32262


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32262.t1 MTR_8g042490 94.894 235 12 0 1 235 1 235 8.63e-160 442
MS.gene32262.t1 MTR_2g073190 75.745 235 56 1 1 234 1 235 5.64e-124 367
MS.gene32262.t1 MTR_1g029380 46.667 210 112 0 18 227 9 218 8.83e-64 199
MS.gene32262.t1 MTR_3g089125 47.418 213 111 1 18 229 9 221 9.58e-63 196
MS.gene32262.t1 MTR_3g080990 43.379 219 122 2 8 224 2 220 7.25e-53 171
MS.gene32262.t1 MTR_6g007633 39.367 221 128 3 14 228 8 228 2.36e-49 162
MS.gene32262.t1 MTR_1g028460 39.437 213 126 2 9 218 1 213 4.97e-49 161
MS.gene32262.t1 MTR_3g090940 37.500 216 132 2 16 228 8 223 8.09e-48 158
MS.gene32262.t1 MTR_3g090950 37.500 216 132 2 16 228 8 223 6.56e-47 156
MS.gene32262.t1 MTR_6g007637 40.278 216 125 3 10 222 5 219 8.58e-47 155
MS.gene32262.t1 MTR_8g099730 38.605 215 130 2 10 222 14 228 4.29e-46 154
MS.gene32262.t1 MTR_6g007623 38.117 223 130 4 14 229 5 226 1.69e-43 147
MS.gene32262.t1 MTR_2g007890 35.000 220 137 3 16 233 14 229 3.33e-40 140
MS.gene32262.t1 MTR_5g067530 37.674 215 130 3 18 230 14 226 3.71e-40 139
MS.gene32262.t1 MTR_8g096320 38.164 207 124 3 18 222 13 217 3.99e-40 139
MS.gene32262.t1 MTR_6g034600 62.602 123 34 2 113 235 1 111 2.31e-39 132
MS.gene32262.t1 MTR_2g436310 40.654 214 116 4 18 226 9 216 8.23e-38 132
MS.gene32262.t1 MTR_3g098910 36.493 211 126 3 16 222 11 217 2.42e-37 133
MS.gene32262.t1 MTR_7g007490 36.744 215 130 3 18 228 14 226 2.55e-37 131
MS.gene32262.t1 MTR_7g405710 39.070 215 128 2 16 229 11 223 5.09e-36 129
MS.gene32262.t1 MTR_4g106990 36.364 198 124 2 34 229 35 232 5.23e-36 127
MS.gene32262.t1 MTR_5g092600 35.961 203 127 2 23 224 18 218 6.19e-36 127
MS.gene32262.t1 MTR_6g007610 34.052 232 135 4 12 228 6 234 1.05e-34 124
MS.gene32262.t1 MTR_7g405730 39.216 204 121 2 18 220 13 214 6.85e-34 123
MS.gene32262.t1 MTR_8g096310 31.944 216 140 4 18 229 13 225 4.31e-33 121
MS.gene32262.t1 MTR_3g098930 36.667 180 113 1 18 197 14 192 1.45e-30 112
MS.gene32262.t1 MTR_8g099730 37.736 159 97 2 66 222 6 164 2.56e-28 106
MS.gene32262.t1 MTR_8g096320 38.926 149 88 2 76 222 15 162 3.52e-26 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32262.t1 AT3G14770 66.524 233 73 3 4 234 6 235 2.06e-104 301
MS.gene32262.t1 AT1G21460 45.498 211 115 0 18 228 9 219 6.29e-63 197
MS.gene32262.t1 AT1G21460 44.444 189 105 0 11 199 2 190 1.17e-51 166
MS.gene32262.t1 AT5G62850 38.636 220 133 2 11 228 5 224 4.20e-51 166
MS.gene32262.t1 AT4G10850 40.807 223 124 4 14 229 10 231 1.24e-49 163
MS.gene32262.t1 AT5G53190 39.713 209 123 2 18 223 10 218 1.21e-48 160
MS.gene32262.t1 AT1G66770 40.845 213 121 3 14 222 10 221 3.65e-46 154
MS.gene32262.t1 AT5G13170 38.389 211 126 2 18 226 15 223 9.64e-45 152
MS.gene32262.t1 AT5G40260 36.866 217 132 4 18 229 12 228 1.43e-42 144
MS.gene32262.t1 AT5G23660 39.409 203 120 2 18 219 15 215 5.75e-42 144
MS.gene32262.t1 AT3G48740 37.727 220 131 3 1 219 1 215 9.84e-42 144
MS.gene32262.t1 AT3G28007 40.654 214 123 4 11 221 5 217 2.47e-41 142
MS.gene32262.t1 AT5G50800 37.557 221 131 4 18 233 13 231 1.03e-40 141
MS.gene32262.t1 AT4G25010 39.640 222 126 5 18 233 13 232 1.45e-40 140
MS.gene32262.t1 AT4G15920 38.164 207 124 2 18 222 9 213 1.61e-40 139
MS.gene32262.t1 AT5G62850 37.368 190 118 1 11 199 5 194 1.54e-39 136
MS.gene32262.t1 AT2G39060 36.364 209 130 2 18 225 13 219 8.14e-39 135
MS.gene32262.t1 AT3G16690 36.058 208 127 4 18 222 9 213 3.04e-36 128
MS.gene32262.t1 AT5G50790 37.383 214 129 3 18 229 13 223 3.66e-36 129
MS.gene32262.t1 AT3G16690 35.484 217 128 5 18 231 44 251 4.50e-36 128
MS.gene32262.t1 AT5G40260 36.747 166 102 2 18 180 12 177 6.94e-32 116
MS.gene32262.t1 AT3G16690 31.206 141 91 4 18 155 9 146 2.17e-14 69.7

Find 40 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATCTCCTTCTTTCTTTA+TGG 0.094340 8.3:+48733434 MS.gene32262:CDS
GCTTACTCCATTTGTGAAAT+TGG 0.125093 8.3:+48732501 MS.gene32262:CDS
TGAATTGTAGACCCACATTT+AGG 0.279401 8.3:+48732777 MS.gene32262:intron
GTAGGTAATATCTTTGCATT+TGG 0.325780 8.3:+48732642 MS.gene32262:intron
TATATCACTTGATAATTTGT+TGG 0.334698 8.3:+48732898 MS.gene32262:CDS
GTTACCACTGTTAATTCAAT+TGG 0.337932 8.3:+48732920 MS.gene32262:CDS
TTTAGGAGAATTATGAGAAA+TGG 0.341950 8.3:+48732794 MS.gene32262:CDS
TTCAATACAGAATAAATATA+TGG 0.362572 8.3:-48732842 None:intergenic
TAGGTAATATCTTTGCATTT+GGG 0.393033 8.3:+48732643 MS.gene32262:intron
TGAACTGTTTGATCTGTTTG+TGG 0.395153 8.3:+48732861 MS.gene32262:CDS
TTGATTTGTTAGGTACCAAA+TGG 0.416862 8.3:+48733594 MS.gene32262:intron
AAAGATGCAGCTGGAATTGC+TGG 0.429368 8.3:+48732525 MS.gene32262:CDS
AAATGGGATAGGTACTGTTC+TGG 0.436030 8.3:+48733611 MS.gene32262:CDS
ATCTTTACCAATTTCACAAA+TGG 0.440430 8.3:-48732508 None:intergenic
TTAGGAGAATTATGAGAAAT+GGG 0.442641 8.3:+48732795 MS.gene32262:CDS
GAGAATGATTGGATTATTGC+TGG 0.448517 8.3:+48733107 MS.gene32262:CDS
GGGTCAACAGAAATGTTCTC+TGG 0.465035 8.3:+48732815 MS.gene32262:CDS
TGTTTGATCTGTTTGTGGTA+TGG 0.483763 8.3:+48732866 MS.gene32262:CDS
TCTCATAATTCTCCTAAATG+TGG 0.499347 8.3:-48732789 None:intergenic
AATGGGATAGGTACTGTTCT+GGG 0.502068 8.3:+48733612 MS.gene32262:CDS
AAATTATCAAGTGATATAAG+AGG 0.509598 8.3:-48732893 None:intergenic
AGAACAGTACCTATCCCATT+TGG 0.512492 8.3:-48733609 None:intergenic
TGATTTGTTAGGTACCAAAT+GGG 0.514371 8.3:+48733595 MS.gene32262:intron
CTCATAATTCTCCTAAATGT+GGG 0.519009 8.3:-48732788 None:intergenic
GTCGAAAGCGAAAGATAAAA+CGG 0.522437 8.3:-48733401 None:intergenic
GAAGGAGATGCTCATTAAGA+AGG 0.533630 8.3:-48733423 None:intergenic
TCAAGCATCCGACTAACATT+CGG 0.541780 8.3:-48733194 None:intergenic
TATTTGTGATCATACTAGTC+GGG 0.546736 8.3:+48733142 MS.gene32262:CDS
GAGTAATCCATAAAGAAAGA+AGG 0.551073 8.3:-48733441 None:intergenic
TTATCCATACGACACTATCA+AGG 0.565472 8.3:-48733697 None:intergenic
TGTTAGGTACCAAATGGGAT+AGG 0.566157 8.3:+48733600 MS.gene32262:intron
CATTCGGCGCATAGAACTAT+CGG 0.567063 8.3:-48733178 None:intergenic
GAAATTGGTAAAGATGCAGC+TGG 0.580425 8.3:+48732516 MS.gene32262:CDS
GAACCCTTGATAGTGTCGTA+TGG 0.586423 8.3:+48733693 MS.gene32262:CDS
ATATTTGTGATCATACTAGT+CGG 0.590837 8.3:+48733141 MS.gene32262:CDS
TCTATGCGCCGAATGTTAGT+CGG 0.595174 8.3:+48733186 MS.gene32262:CDS
AAACTTACGATTATAAACAA+TGG 0.620280 8.3:-48733249 None:intergenic
TACGATTATAAACAATGGAG+AGG 0.620571 8.3:-48733244 None:intergenic
TATCCATACGACACTATCAA+GGG 0.638241 8.3:-48733696 None:intergenic
TGCTCCAATTGAATTAACAG+TGG 0.678257 8.3:-48732924 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTCTAATACTATATA+TGG + chr8.3:48733006-48733025 MS.gene32262:intron 10.0%
!! ATAAAATCATTGAAAATTAG+AGG - chr8.3:48732565-48732584 None:intergenic 15.0%
!! TTCAATACAGAATAAATATA+TGG - chr8.3:48732845-48732864 None:intergenic 15.0%
!!! AAAATTTCATGTATATCTTT+TGG + chr8.3:48732733-48732752 MS.gene32262:intron 15.0%
!!! ATATCTTTTGGTTATTAATT+TGG + chr8.3:48732745-48732764 MS.gene32262:intron 15.0%
!!! ATTAGAAAAATAACCTTTTT+TGG - chr8.3:48732998-48733017 None:intergenic 15.0%
!! AAACTTACGATTATAAACAA+TGG - chr8.3:48733252-48733271 None:intergenic 20.0%
!! AAATGTAGGTATACAAATAT+AGG - chr8.3:48733569-48733588 None:intergenic 20.0%
!! AAATTATCAAGTGATATAAG+AGG - chr8.3:48732896-48732915 None:intergenic 20.0%
!! CTATATAATTTCACAGAAAT+TGG + chr8.3:48733349-48733368 MS.gene32262:intron 20.0%
!! TTATGATAAATTTCTTCACA+TGG + chr8.3:48733034-48733053 MS.gene32262:intron 20.0%
!!! AAACTTTAAGAAACTTACAT+TGG - chr8.3:48732681-48732700 None:intergenic 20.0%
!!! GAATTCAGATTTTATTTTGT+TGG + chr8.3:48733543-48733562 MS.gene32262:intron 20.0%
!!! TATATCACTTGATAATTTGT+TGG + chr8.3:48732898-48732917 MS.gene32262:CDS 20.0%
! ACAAATCAAACACAAAATGT+AGG - chr8.3:48733583-48733602 None:intergenic 25.0%
! ATATTTGTGATCATACTAGT+CGG + chr8.3:48733141-48733160 MS.gene32262:CDS 25.0%
! ATCTTTACCAATTTCACAAA+TGG - chr8.3:48732511-48732530 None:intergenic 25.0%
! ATTCTTGCTTGTTTATTTGT+AGG + chr8.3:48732624-48732643 MS.gene32262:intron 25.0%
! TAGGTAATATCTTTGCATTT+GGG + chr8.3:48732643-48732662 MS.gene32262:intron 25.0%
! TAGTAAAACTCAGTATAACA+TGG - chr8.3:48733292-48733311 None:intergenic 25.0%
! TTAGGAGAATTATGAGAAAT+GGG + chr8.3:48732795-48732814 MS.gene32262:CDS 25.0%
! TTTAGGAGAATTATGAGAAA+TGG + chr8.3:48732794-48732813 MS.gene32262:CDS 25.0%
!! ATTGTCTACTTCTTTTTGAT+AGG + chr8.3:48733083-48733102 MS.gene32262:intron 25.0%
!!! CATTTTGTGTTTGATTTGTT+AGG + chr8.3:48733584-48733603 MS.gene32262:intron 25.0%
AGTTTGTGTAAATTCAAGCT+TGG + chr8.3:48733311-48733330 MS.gene32262:intron 30.0%
CTCATAATTCTCCTAAATGT+GGG - chr8.3:48732791-48732810 None:intergenic 30.0%
GAGTAATCCATAAAGAAAGA+AGG - chr8.3:48733444-48733463 None:intergenic 30.0%
GTAGGTAATATCTTTGCATT+TGG + chr8.3:48732642-48732661 MS.gene32262:intron 30.0%
GTTACCACTGTTAATTCAAT+TGG + chr8.3:48732920-48732939 MS.gene32262:CDS 30.0%
TACGATTATAAACAATGGAG+AGG - chr8.3:48733247-48733266 None:intergenic 30.0%
TATTTGTGATCATACTAGTC+GGG + chr8.3:48733142-48733161 MS.gene32262:CDS 30.0%
TCAGTATAACATGGATATCT+AGG - chr8.3:48733283-48733302 None:intergenic 30.0%
TCTCATAATTCTCCTAAATG+TGG - chr8.3:48732792-48732811 None:intergenic 30.0%
! TTTTGATAGGTGAGAATGAT+TGG + chr8.3:48733096-48733115 MS.gene32262:intron 30.0%
!! TGATTTGTTAGGTACCAAAT+GGG + chr8.3:48733595-48733614 MS.gene32262:intron 30.0%
!! TTGATTTGTTAGGTACCAAA+TGG + chr8.3:48733594-48733613 MS.gene32262:intron 30.0%
AGCATCTCCTTCTTTCTTTA+TGG + chr8.3:48733434-48733453 MS.gene32262:CDS 35.0%
CTATGCTGAGAAACCAAAAA+AGG + chr8.3:48732982-48733001 MS.gene32262:CDS 35.0%
GAGAATGATTGGATTATTGC+TGG + chr8.3:48733107-48733126 MS.gene32262:CDS 35.0%
GCTTACTCCATTTGTGAAAT+TGG + chr8.3:48732501-48732520 MS.gene32262:CDS 35.0%
GTCGAAAGCGAAAGATAAAA+CGG - chr8.3:48733404-48733423 None:intergenic 35.0%
TATCCATACGACACTATCAA+GGG - chr8.3:48733699-48733718 None:intergenic 35.0%
TGAACTGTTTGATCTGTTTG+TGG + chr8.3:48732861-48732880 MS.gene32262:CDS 35.0%
TGAATTGTAGACCCACATTT+AGG + chr8.3:48732777-48732796 MS.gene32262:intron 35.0%
TGCTCCAATTGAATTAACAG+TGG - chr8.3:48732927-48732946 None:intergenic 35.0%
TGTTTGATCTGTTTGTGGTA+TGG + chr8.3:48732866-48732885 MS.gene32262:CDS 35.0%
TTATCCATACGACACTATCA+AGG - chr8.3:48733700-48733719 None:intergenic 35.0%
AGAACAGTACCTATCCCATT+TGG - chr8.3:48733612-48733631 None:intergenic 40.0%
GAAATTGGTAAAGATGCAGC+TGG + chr8.3:48732516-48732535 MS.gene32262:CDS 40.0%
GAAGGAGATGCTCATTAAGA+AGG - chr8.3:48733426-48733445 None:intergenic 40.0%
TCAAGCATCCGACTAACATT+CGG - chr8.3:48733197-48733216 None:intergenic 40.0%
TGTTAGGTACCAAATGGGAT+AGG + chr8.3:48733600-48733619 MS.gene32262:intron 40.0%
! AAATGGGATAGGTACTGTTC+TGG + chr8.3:48733611-48733630 MS.gene32262:CDS 40.0%
! AATGGGATAGGTACTGTTCT+GGG + chr8.3:48733612-48733631 MS.gene32262:CDS 40.0%
!! GGATTATTGCTGGCTGTTTT+CGG + chr8.3:48733117-48733136 MS.gene32262:CDS 40.0%
AAAGATGCAGCTGGAATTGC+TGG + chr8.3:48732525-48732544 MS.gene32262:CDS 45.0%
CATTCGGCGCATAGAACTAT+CGG - chr8.3:48733181-48733200 None:intergenic 45.0%
GGGTCAACAGAAATGTTCTC+TGG + chr8.3:48732815-48732834 MS.gene32262:CDS 45.0%
TCTATGCGCCGAATGTTAGT+CGG + chr8.3:48733186-48733205 MS.gene32262:CDS 45.0%
! AATTGGAGCAGCTTTTCAGC+TGG + chr8.3:48732937-48732956 MS.gene32262:CDS 45.0%
!! GAACCCTTGATAGTGTCGTA+TGG + chr8.3:48733693-48733712 MS.gene32262:CDS 45.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 48732486 48733719 48732486 ID=MS.gene32262
chr8.3 mRNA 48732486 48733719 48732486 ID=MS.gene32262.t1;Parent=MS.gene32262
chr8.3 exon 48732486 48732546 48732486 ID=MS.gene32262.t1.exon1;Parent=MS.gene32262.t1
chr8.3 CDS 48732486 48732546 48732486 ID=cds.MS.gene32262.t1;Parent=MS.gene32262.t1
chr8.3 exon 48732646 48732682 48732646 ID=MS.gene32262.t1.exon2;Parent=MS.gene32262.t1
chr8.3 CDS 48732646 48732682 48732646 ID=cds.MS.gene32262.t1;Parent=MS.gene32262.t1
chr8.3 exon 48732787 48733003 48732787 ID=MS.gene32262.t1.exon3;Parent=MS.gene32262.t1
chr8.3 CDS 48732787 48733003 48732787 ID=cds.MS.gene32262.t1;Parent=MS.gene32262.t1
chr8.3 exon 48733105 48733263 48733105 ID=MS.gene32262.t1.exon4;Parent=MS.gene32262.t1
chr8.3 CDS 48733105 48733263 48733105 ID=cds.MS.gene32262.t1;Parent=MS.gene32262.t1
chr8.3 exon 48733365 48733484 48733365 ID=MS.gene32262.t1.exon5;Parent=MS.gene32262.t1
chr8.3 CDS 48733365 48733484 48733365 ID=cds.MS.gene32262.t1;Parent=MS.gene32262.t1
chr8.3 exon 48733606 48733719 48733606 ID=MS.gene32262.t1.exon6;Parent=MS.gene32262.t1
chr8.3 CDS 48733606 48733719 48733606 ID=cds.MS.gene32262.t1;Parent=MS.gene32262.t1
Gene Sequence

>MS.gene32262

ATGTCTCTGTTTAATGCTTACTCCATTTGTGAAATTGGTAAAGATGCAGCTGGAATTGCTGGTACTACATTTTCTTCCTCTAATTTTCAATGATTTTATTACATTTAACATTTTTAACTTATCTTTATCTTGATCTCAATTCTTGCTTGTTTATTTGTAGGTAATATCTTTGCATTTGGGTTATTTCTATCTCCAATGTAAGTTTCTTAAAGTTTTATTTTTGTTAAATTAAAAATTAAGCTTAGAAAAAATTTCATGTATATCTTTTGGTTATTAATTTGGATTATATTGTGAATTGTAGACCCACATTTAGGAGAATTATGAGAAATGGGTCAACAGAAATGTTCTCTGGATTGCCATATATTTATTCTGTATTGAACTGTTTGATCTGTTTGTGGTATGGAACTCCTCTTATATCACTTGATAATTTGTTGGTTACCACTGTTAATTCAATTGGAGCAGCTTTTCAGCTGGTGTACATTTTCTTATTCTTAATCTATGCTGAGAAACCAAAAAAGGTTATTTTTCTAATACTATATATGGAAAGTTTATGATAAATTTCTTCACATGGATTTTGAAGTGTTACTAAGATGTGATATTGTCTACTTCTTTTTGATAGGTGAGAATGATTGGATTATTGCTGGCTGTTTTCGGTATATTTGTGATCATACTAGTCGGGAGCTTGAAAATAACCGATAGTTCTATGCGCCGAATGTTAGTCGGATGCTTGAGTTGTGCTTCTCTCGTTTCAATGTTTGCCTCTCCATTGTTTATAATCGTAAGTTTGACATTTTCCTAGATATCCATGTTATACTGAGTTTTACTAGTTTGTGTAAATTCAAGCTTGGTGAAATTGTTTATATCTATATAATTTCACAGAAATTGGTCATTCGCACGAAGAGTGTTGAGTTTATGCCGTTTTATCTTTCGCTTTCGACCTTCTTAATGAGCATCTCCTTCTTTCTTTATGGATTACTCAGTGACGATGTCTTCATTTATGTAAGAGTCTTTTCGTCTCATTTCTCTGTTTCTTTCTGAAATATTGATAACAGTTTTAGAATTCAGATTTTATTTTGTTGGCCTATATTTGTATACCTACATTTTGTGTTTGATTTGTTAGGTACCAAATGGGATAGGTACTGTTCTGGGAATGATACAATTGATTTTATACTTCTATTACAAGAGTTCATCTAGCGATGACTCTACAGAACCCTTGATAGTGTCGTATGGATAA

Protein sequence

>MS.gene32262.t1

MSLFNAYSICEIGKDAAGIAGNIFAFGLFLSPIPTFRRIMRNGSTEMFSGLPYIYSVLNCLICLWYGTPLISLDNLLVTTVNSIGAAFQLVYIFLFLIYAEKPKKVRMIGLLLAVFGIFVIILVGSLKITDSSMRRMLVGCLSCASLVSMFASPLFIIKLVIRTKSVEFMPFYLSLSTFLMSISFFLYGLLSDDVFIYVPNGIGTVLGMIQLILYFYYKSSSSDDSTEPLIVSYG