AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32551

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32551.t1 MTR_5g080010 95.702 605 19 1 250 847 12 616 0.0 1182
MS.gene32551.t1 MTR_5g080010 95.411 414 19 0 250 663 12 425 0.0 761
MS.gene32551.t1 MTR_3g067935 53.698 622 242 13 252 837 14 625 0.0 573
MS.gene32551.t1 MTR_5g080020 99.138 232 2 0 1 232 1 232 4.02e-164 476
MS.gene32551.t1 MTR_2g097290 43.290 462 225 6 395 826 253 707 5.69e-105 340
MS.gene32551.t1 MTR_7g061840 37.020 651 288 19 252 835 14 609 4.60e-100 324
MS.gene32551.t1 MTR_2g097290 36.973 403 217 6 395 767 253 648 2.26e-62 224
MS.gene32551.t1 MTR_2g097290 31.884 207 120 4 395 583 253 456 6.49e-15 79.0
MS.gene32551.t1 MTR_4g078640 27.586 232 112 5 8 228 13 199 1.72e-13 75.1
MS.gene32551.t1 MTR_4g070380 26.522 230 113 5 10 228 15 199 9.63e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32551.t1 AT5G13820 44.691 631 292 13 249 837 12 627 1.71e-146 446
MS.gene32551.t1 AT5G13820 44.691 631 292 13 249 837 12 627 1.71e-146 446
MS.gene32551.t1 AT3G12560 42.789 631 285 14 243 835 7 599 5.55e-136 418
MS.gene32551.t1 AT3G12560 42.789 631 285 14 243 835 7 599 5.55e-136 418
MS.gene32551.t1 AT5G13830 69.828 232 57 4 1 230 1 221 2.23e-105 324
MS.gene32551.t1 AT3G46590 39.032 620 262 23 252 843 14 545 3.67e-97 314
MS.gene32551.t1 AT3G46590 38.808 621 263 24 252 843 14 546 2.46e-96 312
MS.gene32551.t1 AT3G46590 38.808 621 257 24 252 843 14 540 2.31e-95 310
MS.gene32551.t1 AT3G46590 39.189 592 244 23 280 843 2 505 2.92e-91 297
MS.gene32551.t1 AT5G59430 41.727 417 186 11 427 834 188 556 1.02e-76 260
MS.gene32551.t1 AT5G59430 41.809 409 182 10 427 826 188 549 5.35e-76 258
MS.gene32551.t1 AT5G59430 41.809 409 182 10 427 826 188 549 5.35e-76 258
MS.gene32551.t1 AT5G59430 41.809 409 182 10 427 826 188 549 5.35e-76 258
MS.gene32551.t1 AT5G59430 41.809 409 182 10 427 826 187 548 7.70e-76 258
MS.gene32551.t1 AT3G53790 43.195 338 124 8 497 826 119 396 3.82e-65 224
MS.gene32551.t1 AT3G46590 34.307 411 187 19 252 637 14 366 3.08e-39 150

Find 206 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCTCCAATTACTTTCTT+AGG 0.109494 5.1:-62227916 None:intergenic
GACATGTGTCCGTTGGTTTC+TGG 0.154573 5.1:+62224973 MS.gene32551:CDS
TGCAACTGTTGCTTCATTAA+AGG 0.170101 5.1:+62228130 MS.gene32551:CDS
ATGATGACGAAAACTTTATT+AGG 0.242004 5.1:+62227342 MS.gene32551:CDS
GGAGCTATTGGATAGGGTTT+TGG 0.246722 5.1:+62230188 MS.gene32551:CDS
AATTACTTTCTTAGGGGAAT+TGG 0.254177 5.1:-62227908 None:intergenic
GGAAACAATTCCCCACAAAA+TGG 0.254603 5.1:-62227266 None:intergenic
TAAACAATGGTTTACAAATC+TGG 0.268314 5.1:-62225313 None:intergenic
TGGGTTGTGCTCCCGGTTCA+TGG 0.269114 5.1:+62222923 MS.gene32551:CDS
CCATCTTGTAGTCAAGCTTT+TGG 0.276645 5.1:+62225167 MS.gene32551:CDS
AATTCCCCTAAGAAAGTAAT+TGG 0.278506 5.1:+62227910 MS.gene32551:CDS
GAAGCAACAGTTGCAGTTTC+GGG 0.282983 5.1:-62228122 None:intergenic
CATAACGGTGGTTCCGTTCT+CGG 0.292575 5.1:+62222979 MS.gene32551:CDS
TGCAAAAGTTCGCGTTTCTA+AGG 0.302423 5.1:+62227954 MS.gene32551:CDS
TTACTTACTCTTACAGATCT+TGG 0.307105 5.1:-62226760 None:intergenic
TGGTTTGTGTGCAGGATAAA+TGG 0.309116 5.1:+62230103 MS.gene32551:intron
TGAAGCAACAGTTGCAGTTT+CGG 0.311212 5.1:-62228123 None:intergenic
AGTTTCGCATCGAGTCATTC+AGG 0.318139 5.1:+62228075 MS.gene32551:CDS
TTCAAATTGGAGAATAGGTT+TGG 0.318263 5.1:+62227116 MS.gene32551:CDS
TGCTCCCGGTTCATGGCTTC+AGG 0.324628 5.1:+62222930 MS.gene32551:CDS
GACGAAGGTCTTGTGGCTTT+AGG 0.325086 5.1:-62225356 None:intergenic
CTCGACTTGGGTTGTGCTCC+CGG 0.329436 5.1:+62222916 MS.gene32551:CDS
TCCTCCTTGCTTACAAATAC+TGG 0.330363 5.1:+62229085 MS.gene32551:CDS
ACATTGCACGCAAAATAATT+AGG 0.331937 5.1:+62227411 MS.gene32551:CDS
CGAAATCGCTCTTCTGTTAA+CGG 0.335512 5.1:-62226987 None:intergenic
GAGGCGGTGATGACTATAAT+AGG 0.341788 5.1:+62228316 MS.gene32551:CDS
GGGAGGAATGCTAGTAAGTT+AGG 0.342627 5.1:+62227311 MS.gene32551:CDS
AGGGATTTCTGTTGCCAAAC+AGG 0.342636 5.1:+62229060 MS.gene32551:CDS
GGAACTCCCTCCATAGATTC+AGG 0.342756 5.1:+62229040 MS.gene32551:CDS
TGGAGCAACCTTCCAGTGTT+TGG 0.343786 5.1:-62227453 None:intergenic
TTCCTCCAATTACTTTCTTA+GGG 0.346950 5.1:-62227915 None:intergenic
GCCAGTTCCTCAGGAGCTAT+TGG 0.348630 5.1:+62230176 MS.gene32551:CDS
CTTGGACATCTAGGTACCTT+AGG 0.349158 5.1:-62226742 None:intergenic
TCAACTTCAGACACAGAAAA+TGG 0.357936 5.1:-62229292 None:intergenic
CAATTCTGATGGTGATAATC+TGG 0.360220 5.1:+62225080 MS.gene32551:CDS
TTTGTCTTTGTGCAGCAAAA+AGG 0.367282 5.1:+62224931 MS.gene32551:intron
GCATGTTGGTGTTCTTGTTA+AGG 0.368440 5.1:+62228348 MS.gene32551:CDS
CGGTTCATCCGTTCTCGACT+TGG 0.371504 5.1:+62222903 MS.gene32551:CDS
CGACAAGTCATTGTGATTCC+AGG 0.376454 5.1:+62223028 MS.gene32551:CDS
GCTTCTTTGTAGAAGAAATC+TGG 0.384459 5.1:-62222805 None:intergenic
AACCTTCCAGTGTTTGGAAT+TGG 0.390971 5.1:-62227447 None:intergenic
CGCGTTTCTAAGGATTCTAA+TGG 0.394563 5.1:+62227964 MS.gene32551:CDS
TCCAATAGCTCCTGAGGAAC+TGG 0.395946 5.1:-62230177 None:intergenic
AGCTACCTGAAGCCATGAAC+CGG 0.396973 5.1:-62222935 None:intergenic
AGGTCTCTAAGCTGTTGTTT+AGG 0.397391 5.1:-62223084 None:intergenic
TTCAGACTTCACATTATTGT+TGG 0.402330 5.1:-62227090 None:intergenic
TCAGGTAGCTTGTCAAAGCT+TGG 0.404283 5.1:+62222948 MS.gene32551:CDS
AGCTCGCTCGGCTTTCAAAC+TGG 0.408381 5.1:+62222846 MS.gene32551:CDS
GCTTCTCCAGTTGTTTGTGC+TGG 0.408670 5.1:+62228475 MS.gene32551:CDS
AAGCAAGGAGGAACCCTGTT+TGG 0.410467 5.1:-62229074 None:intergenic
TGTTCACAATCTTTCAACTT+TGG 0.411776 5.1:-62227473 None:intergenic
AACAGAAATCCCTGAATCTA+TGG 0.411949 5.1:-62229050 None:intergenic
CAAGTCGAGAACGGATGAAC+CGG 0.413108 5.1:-62222902 None:intergenic
GGATCACCAGCACAAACAAC+TGG 0.414617 5.1:-62228481 None:intergenic
CATGTTGGTGTTCTTGTTAA+GGG 0.416802 5.1:+62228349 MS.gene32551:CDS
GGAACGTCGACGTAAAGTTC+TGG 0.420090 5.1:-62228101 None:intergenic
GCTTGACATGCTGCTTGGAT+TGG 0.423506 5.1:-62230236 None:intergenic
CAGGTAGCTTGTCAAAGCTT+GGG 0.423945 5.1:+62222949 MS.gene32551:CDS
TGACATGCTGCTTGGATTGG+TGG 0.426360 5.1:-62230233 None:intergenic
GAAACAATTCCCCACAAAAT+GGG 0.429990 5.1:-62227265 None:intergenic
CATCACGAGGCTGACAGTTC+AGG 0.432205 5.1:+62227179 MS.gene32551:CDS
TAATGGATTCAGGGACTATC+AGG 0.432252 5.1:+62226717 MS.gene32551:intron
GAGAGGACAGCCAGTTCCTC+AGG 0.432830 5.1:+62230167 MS.gene32551:CDS
TTGATGCTTGACATGCTGCT+TGG 0.441541 5.1:-62230241 None:intergenic
CGAGAACGGAACCACCGTTA+TGG 0.447208 5.1:-62222978 None:intergenic
CATTGATCATAAGAAAGTTA+AGG 0.448190 5.1:+62223002 MS.gene32551:CDS
GATAAACTAACACAAGATTC+CGG 0.452961 5.1:+62229166 MS.gene32551:CDS
CTTACAGATCTTGGACATCT+AGG 0.453561 5.1:-62226751 None:intergenic
GTTCATGCAGTTGAGGAAGT+TGG 0.454012 5.1:+62229322 MS.gene32551:CDS
CAAGGGAAGAAGCTGATTCA+TGG 0.454467 5.1:-62229129 None:intergenic
ATCCGTCTGGTTCAGATGAT+GGG 0.455535 5.1:+62225124 MS.gene32551:CDS
TTCCTTGAAAAGGAAGCATT+GGG 0.461169 5.1:-62227287 None:intergenic
TCCTCAGGAGCTATTGGATA+GGG 0.470302 5.1:+62230182 MS.gene32551:CDS
ATTCCTCCCATTCCTTGAAA+AGG 0.474110 5.1:-62227297 None:intergenic
GCTTCTGTTTGCAATTCTGA+TGG 0.478252 5.1:+62225069 MS.gene32551:CDS
TAGGACTTATGTAGACTTGA+AGG 0.479281 5.1:+62229470 MS.gene32551:CDS
GGCTGTCCTCTCCTTTGTTG+AGG 0.480024 5.1:-62230156 None:intergenic
GCAGCTTTGTCATTTGAGCT+AGG 0.481161 5.1:+62225012 MS.gene32551:CDS
ATTCCTTGAAAAGGAAGCAT+TGG 0.481452 5.1:-62227288 None:intergenic
TTGGATTTGTCTAGGTCTCT+AGG 0.484229 5.1:+62229019 MS.gene32551:intron
GCTTAGAGACCTTTCTCCTA+AGG 0.487966 5.1:+62223095 MS.gene32551:CDS
GCAGTTGAGGAAGTTGGAAC+TGG 0.488859 5.1:+62229328 MS.gene32551:CDS
AGAGGAAGAAATTATCAGAC+AGG 0.491022 5.1:+62227839 MS.gene32551:CDS
GAACTACTTGCATCTTTAGC+CGG 0.491484 5.1:+62226909 MS.gene32551:CDS
CAGCTTTGTCATTTGAGCTA+GGG 0.492065 5.1:+62225013 MS.gene32551:CDS
TAAACCATTGTTTACAAAGT+CGG 0.493601 5.1:+62225322 MS.gene32551:CDS
CTGGAATCACAATGACTTGT+CGG 0.494894 5.1:-62223027 None:intergenic
CTCTCTCGACGAAGGTCTTG+TGG 0.500379 5.1:-62225363 None:intergenic
GAGAAACATCTTGAGAGTGC+TGG 0.500393 5.1:+62227038 MS.gene32551:CDS
CGTGAAGGTCTAAAAGCCTT+CGG 0.501813 5.1:-62227386 None:intergenic
GATCCGTCTGGTTCAGATGA+TGG 0.502066 5.1:+62225123 MS.gene32551:CDS
TGGTCCAGTGTTCTGATTCA+CGG 0.506571 5.1:-62229239 None:intergenic
ACAGAAGAGCGATTTCGTAA+TGG 0.507986 5.1:+62226993 MS.gene32551:CDS
AGACACAGAAAATGGTCTCC+TGG 0.509618 5.1:-62229284 None:intergenic
AGCTTGTACAGCGTCGAACC+AGG 0.512219 5.1:+62229266 MS.gene32551:CDS
GGTTCATCCGTTCTCGACTT+GGG 0.514344 5.1:+62222904 MS.gene32551:CDS
AACAGAACTCTTCGTCAGAC+TGG 0.514395 5.1:+62228391 MS.gene32551:CDS
CCGCTCAAGTTGGCTAAAGA+AGG 0.515374 5.1:-62227230 None:intergenic
CAAGAGTGGCCATCCTCGAC+TGG 0.517246 5.1:-62226879 None:intergenic
TGCGACCCTCGAGTCATAAG+AGG 0.517455 5.1:+62227770 MS.gene32551:CDS
GAGATCTATTTGATATGCCA+AGG 0.520570 5.1:+62225383 MS.gene32551:CDS
AGAAACATCTTGAGAGTGCT+GGG 0.523924 5.1:+62227039 MS.gene32551:CDS
GAACTCCCTCCATAGATTCA+GGG 0.526972 5.1:+62229041 MS.gene32551:CDS
GATTGTGAACATTCTCGCTA+TGG 0.532088 5.1:+62227485 MS.gene32551:CDS
ACTGACTAACCTTAGGAGAA+AGG 0.537203 5.1:-62223104 None:intergenic
CACAAGTGGTTGGACAACAT+TGG 0.539637 5.1:-62230265 None:intergenic
AAGGAGCGATGTGATCCGTC+TGG 0.541102 5.1:+62225111 MS.gene32551:CDS
TCAAGTCTACATAAGTCCTA+TGG 0.543438 5.1:-62229467 None:intergenic
GGTCCAGTGTTCTGATTCAC+GGG 0.543575 5.1:-62229238 None:intergenic
AGGGATGCGAGCGCTAGACT+TGG 0.543707 5.1:+62225032 MS.gene32551:CDS
AATGCCTCAGTACTATGAGC+TGG 0.546437 5.1:-62229205 None:intergenic
AGGTCTAAAAGCCTTCGGTT+TGG 0.548415 5.1:-62227381 None:intergenic
GCTAGACTTGGCTGTTGGCA+AGG 0.550263 5.1:+62225044 MS.gene32551:CDS
CTTATGATCAATGCCGAGAA+CGG 0.550591 5.1:-62222992 None:intergenic
GGGATTTCTGTTGCCAAACA+GGG 0.550679 5.1:+62229061 MS.gene32551:CDS
TTGGACATCTAGGTACCTTA+GGG 0.553309 5.1:-62226741 None:intergenic
AGATAAACTCGGTTTCGTGT+TGG 0.553459 5.1:+62228435 MS.gene32551:CDS
ATGTAGCATCTCAGCCTACC+AGG 0.553875 5.1:+62228512 MS.gene32551:CDS
AGTGTCACACTCTGTTGAAG+AGG 0.554973 5.1:+62227821 MS.gene32551:CDS
TCTACATAATCACAAGTGGT+TGG 0.557057 5.1:-62230275 None:intergenic
TTTAATGTTACCGCTCAAGT+TGG 0.557954 5.1:-62227240 None:intergenic
AGCACTTGTTCATGCAGTTG+AGG 0.558933 5.1:+62229315 MS.gene32551:CDS
TATACTGTAGCAGCTATAGC+AGG 0.560329 5.1:+62225517 MS.gene32551:CDS
AGTCATAACATCCGCCGCAA+CGG 0.560413 5.1:-62223058 None:intergenic
TGATAATCTGGACGAGGTCA+AGG 0.564757 5.1:+62225092 MS.gene32551:CDS
TCCGTCTGGTTCAGATGATG+GGG 0.566114 5.1:+62225125 MS.gene32551:CDS
TTCCAATTCCAAACACTGGA+AGG 0.567244 5.1:+62227445 MS.gene32551:CDS
ACTTTCAGACATGTGTCCGT+TGG 0.567563 5.1:+62224966 MS.gene32551:CDS
ACTCTCCTTCAGCAACTCAC+CGG 0.568548 5.1:-62226928 None:intergenic
TGGAACAGAAACTAGCGCTC+CGG 0.569116 5.1:-62229185 None:intergenic
GGTTCGACGCTGTACAAGCT+CGG 0.569476 5.1:-62229263 None:intergenic
GACAAGTCATTGTGATTCCA+GGG 0.570491 5.1:+62223029 MS.gene32551:CDS
TTTAGCCGGTGAGTTGCTGA+AGG 0.574743 5.1:+62226923 MS.gene32551:CDS
CAGGGACTATCAGGTCCCTA+AGG 0.575068 5.1:+62226726 MS.gene32551:intron
TCAACTTTCGCTCACCTGGT+AGG 0.576936 5.1:-62228526 None:intergenic
ACCGCCAGTATTTGTAAGCA+AGG 0.577016 5.1:-62229089 None:intergenic
TCCCCTAAGAAAGTAATTGG+AGG 0.577061 5.1:+62227913 MS.gene32551:CDS
GCAAAATAATTAGGAAGCGG+TGG 0.577586 5.1:+62227420 MS.gene32551:CDS
GTCTTCCTCTTATGACTCGA+GGG 0.577638 5.1:-62227775 None:intergenic
AGTCTTCCTCTTATGACTCG+AGG 0.577787 5.1:-62227776 None:intergenic
TACAAAGAAGCTCAACGACT+CGG 0.578896 5.1:+62222817 MS.gene32551:CDS
CACGCAAAATAATTAGGAAG+CGG 0.581824 5.1:+62227417 MS.gene32551:CDS
GTGATGACTATAATAGGAGG+TGG 0.582455 5.1:+62228322 MS.gene32551:CDS
GGAGAAGCTTGAGAAGAGCT+AGG 0.582931 5.1:-62228460 None:intergenic
CGACGCTGTACAAGCTCGGA+TGG 0.583494 5.1:-62229259 None:intergenic
CGAGCGCTAGACTTGGCTGT+TGG 0.585409 5.1:+62225039 MS.gene32551:CDS
ACGGAACCACCGTTATGGAA+TGG 0.585785 5.1:-62222973 None:intergenic
TCCTCCAATTACTTTCTTAG+GGG 0.586162 5.1:-62227914 None:intergenic
GCTACCTGAAGCCATGAACC+GGG 0.589112 5.1:-62222934 None:intergenic
TGAGATGCTACATGGTGAGA+AGG 0.589368 5.1:-62228502 None:intergenic
TTCAAGAAACCAGTCGAGGA+TGG 0.590011 5.1:+62226870 MS.gene32551:CDS
ATCAGATGCATTGCTAGAGG+TGG 0.590241 5.1:-62226958 None:intergenic
ATCTCTCCTCAACAAAGGAG+AGG 0.592039 5.1:+62230150 MS.gene32551:CDS
CACACCGACTTTGTAAACAA+TGG 0.592308 5.1:-62225326 None:intergenic
CAAAGTTTGCACCAAACCGA+AGG 0.594191 5.1:+62227370 MS.gene32551:CDS
TTCCTCAGGAGCTATTGGAT+AGG 0.595354 5.1:+62230181 MS.gene32551:CDS
TATCAGATATAGTGTCAGCA+AGG 0.595686 5.1:-62229147 None:intergenic
TTTATAGAGGACAGTTATGG+AGG 0.597438 5.1:+62228297 MS.gene32551:intron
AGAAATCCCTGAATCTATGG+AGG 0.598839 5.1:-62229047 None:intergenic
CCAAAAGCTTGACTACAAGA+TGG 0.599176 5.1:-62225167 None:intergenic
AACATCAGATGCATTGCTAG+AGG 0.601082 5.1:-62226961 None:intergenic
CTGAACTGTCAGCCTCGTGA+TGG 0.601669 5.1:-62227178 None:intergenic
CAGCACAAATTAATCAAACC+CGG 0.601707 5.1:+62222883 MS.gene32551:CDS
ACTCGGCTACGTAGCTCGCT+CGG 0.602505 5.1:+62222834 MS.gene32551:CDS
GAGGAAGAAATTATCAGACA+GGG 0.603590 5.1:+62227840 MS.gene32551:CDS
TTGAGGAAGTTGGAACTGGA+AGG 0.604280 5.1:+62229332 MS.gene32551:CDS
AAACAATTCCCCACAAAATG+GGG 0.608737 5.1:-62227264 None:intergenic
GGACTTCCAATTCCAAACAC+TGG 0.611770 5.1:+62227441 MS.gene32551:CDS
AGCACAACCCAAGTCGAGAA+CGG 0.612291 5.1:-62222911 None:intergenic
AAGTCGAGAACGGATGAACC+GGG 0.615199 5.1:-62222901 None:intergenic
ATAGAGGACAGTTATGGAGG+CGG 0.617071 5.1:+62228300 MS.gene32551:intron
ACCCTATCCAATAGCTCCTG+AGG 0.621261 5.1:-62230183 None:intergenic
TCGATGCTAACTCATGGTGG+AGG 0.623580 5.1:+62227865 MS.gene32551:CDS
TCTTTCTACATAATCACAAG+TGG 0.623634 5.1:-62230279 None:intergenic
TTGCGTGCAATGTATCGTGA+AGG 0.623664 5.1:-62227401 None:intergenic
AGCTTGGGTCCATTCCATAA+CGG 0.625753 5.1:+62222964 MS.gene32551:CDS
TGGTAGGCTGAGATGCTACA+TGG 0.626444 5.1:-62228510 None:intergenic
CCTTCTTTAGCCAACTTGAG+CGG 0.626589 5.1:+62227230 MS.gene32551:CDS
ATGTTGGTGTTCTTGTTAAG+GGG 0.626831 5.1:+62228350 MS.gene32551:CDS
AGGGGTTCGATGCTAACTCA+TGG 0.630655 5.1:+62227859 MS.gene32551:CDS
GTAGTTCAAAAGCACAAGAG+TGG 0.631884 5.1:-62226893 None:intergenic
CAGGGTCAAAACCGTTGCGG+CGG 0.633532 5.1:+62223047 MS.gene32551:CDS
ATCAGATATAGTGTCAGCAA+GGG 0.636117 5.1:-62229146 None:intergenic
AAATAGATCTCTCTCGACGA+AGG 0.650218 5.1:-62225371 None:intergenic
AAAGAAAATATAGATAAACT+CGG 0.652207 5.1:+62228424 MS.gene32551:CDS
TTGGAGAGTGAAGATGCCAA+AGG 0.652727 5.1:+62225186 MS.gene32551:CDS
TCCTTGCTTACAAATACTGG+CGG 0.652781 5.1:+62229088 MS.gene32551:CDS
GTTCCCGTGAATCAGAACAC+TGG 0.655650 5.1:+62229235 MS.gene32551:CDS
TGATGGTGATAATCTGGACG+AGG 0.660852 5.1:+62225086 MS.gene32551:CDS
GAAATCCCTGAATCTATGGA+GGG 0.665973 5.1:-62229046 None:intergenic
ATAGGAGGTGGAGTGCATGT+TGG 0.667170 5.1:+62228334 MS.gene32551:CDS
GCGGTGATGACTATAATAGG+AGG 0.667399 5.1:+62228319 MS.gene32551:CDS
TTCCAGGGTCAAAACCGTTG+CGG 0.667905 5.1:+62223044 MS.gene32551:CDS
TGTTCCAGCTCATAGTACTG+AGG 0.671950 5.1:+62229201 MS.gene32551:CDS
TGCGTTCAAGAAACCAGTCG+AGG 0.672753 5.1:+62226866 MS.gene32551:CDS
TTGGGTCCATTCCATAACGG+TGG 0.676529 5.1:+62222967 MS.gene32551:CDS
ATTCATGGTTCATGTCAACA+AGG 0.686818 5.1:-62229114 None:intergenic
CCGTCTGGTTCAGATGATGG+GGG 0.690099 5.1:+62225126 MS.gene32551:CDS
CATTGTTTACAAAGTCGGTG+TGG 0.693178 5.1:+62225327 MS.gene32551:CDS
AGGAAGAAATTATCAGACAG+GGG 0.702812 5.1:+62227841 MS.gene32551:CDS
AATGGAGAGATGGCTAAGAG+CGG 0.705499 5.1:+62222778 None:intergenic
GGTTCGATGCTAACTCATGG+TGG 0.706099 5.1:+62227862 MS.gene32551:CDS
GTCGTGATTCCAGAAACCAA+CGG 0.709220 5.1:-62224982 None:intergenic
GTAATTGGAGGAAATAACCG+TGG 0.709826 5.1:+62227925 MS.gene32551:CDS
GCCAGTATTTGTAAGCAAGG+AGG 0.726150 5.1:-62229086 None:intergenic
AGAAGTTTCAAACCATCACG+AGG 0.743753 5.1:+62227166 MS.gene32551:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.1 gene 62222787 62230296 62222787 ID=MS.gene32551
chr5.1 mRNA 62222787 62230296 62222787 ID=MS.gene32551.t1;Parent=MS.gene32551
chr5.1 exon 62222787 62223116 62222787 ID=MS.gene32551.t1.exon1;Parent=MS.gene32551.t1
chr5.1 CDS 62222787 62223116 62222787 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
chr5.1 exon 62224946 62225207 62224946 ID=MS.gene32551.t1.exon2;Parent=MS.gene32551.t1
chr5.1 CDS 62224946 62225207 62224946 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
chr5.1 exon 62225306 62225404 62225306 ID=MS.gene32551.t1.exon3;Parent=MS.gene32551.t1
chr5.1 CDS 62225306 62225404 62225306 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
chr5.1 exon 62225481 62225538 62225481 ID=MS.gene32551.t1.exon4;Parent=MS.gene32551.t1
chr5.1 CDS 62225481 62225538 62225481 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
chr5.1 exon 62226729 62226774 62226729 ID=MS.gene32551.t1.exon5;Parent=MS.gene32551.t1
chr5.1 CDS 62226729 62226774 62226729 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
chr5.1 exon 62226858 62227506 62226858 ID=MS.gene32551.t1.exon6;Parent=MS.gene32551.t1
chr5.1 CDS 62226858 62227506 62226858 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
chr5.1 exon 62227761 62227985 62227761 ID=MS.gene32551.t1.exon7;Parent=MS.gene32551.t1
chr5.1 CDS 62227761 62227985 62227761 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
chr5.1 exon 62228072 62228151 62228072 ID=MS.gene32551.t1.exon8;Parent=MS.gene32551.t1
chr5.1 CDS 62228072 62228151 62228072 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
chr5.1 exon 62228304 62228533 62228304 ID=MS.gene32551.t1.exon9;Parent=MS.gene32551.t1
chr5.1 CDS 62228304 62228533 62228304 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
chr5.1 exon 62229033 62229353 62229033 ID=MS.gene32551.t1.exon10;Parent=MS.gene32551.t1
chr5.1 CDS 62229033 62229353 62229033 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
chr5.1 exon 62229428 62229491 62229428 ID=MS.gene32551.t1.exon11;Parent=MS.gene32551.t1
chr5.1 CDS 62229428 62229491 62229428 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
chr5.1 exon 62230117 62230296 62230117 ID=MS.gene32551.t1.exon12;Parent=MS.gene32551.t1
chr5.1 CDS 62230117 62230296 62230117 ID=cds.MS.gene32551.t1;Parent=MS.gene32551.t1
Gene Sequence

>MS.gene32551

ATGGCTAAGAGCGGAGCACCAGATTTCTTCTACAAAGAAGCTCAACGACTCGGCTACGTAGCTCGCTCGGCTTTCAAACTGGTTCAAATCCAAAAACAGCACAAATTAATCAAACCCGGTTCATCCGTTCTCGACTTGGGTTGTGCTCCCGGTTCATGGCTTCAGGTAGCTTGTCAAAGCTTGGGTCCATTCCATAACGGTGGTTCCGTTCTCGGCATTGATCATAAGAAAGTTAAGGTTCCGACAAGTCATTGTGATTCCAGGGTCAAAACCGTTGCGGCGGATGTTATGACTCTTCCTAAACAACAGCTTAGAGACCTTTCTCCTAAGGTTAGTCAGTTAGACCGTTCTTTTAACTGTATTAGTTAGTGACTTTGTTTTTTGAGACGCAAACACCTGACACGATACTGACACATTGCCATGAGTAATAACTTGTGCAAATGGAAGTTATTAAATGTAATCACATGCGTTGGTATCATGTTGGCGTATGATACTAATAGGTGTCGAATGCCGAACACACATTCAATTTGAAGTAAGTTAGTTTGTTAGGTCGTTTTGTTACTTATTAGTTAGTTAGATACAAACAAACCAACTCTTCGATTGTGAACTACAAAATGATCATTGGTGTTGCAAAATGTTAATCAGGTTCGTTCTTAATATTTGTAATGACCGCGTAATGAAATTAAGTTCATAATGCTATCTTTATTGATTTGAAACACGAAAATGTTAGAGATGCATTTGATAACAGAGGAACAAGCTTGACTAATACTTTGAAATTTAGGGATCATTTTAGAATTTTGATTATATGGAGGACTAGGTTGATCCGGTCGTGCAATTATTTTTTGTTTGAAAAGACCAAAGTTAATTCTTAGTGTGTGTTTGGTTCTACGGTGAGAAAAATTGATTTTGAGTGAATTGATTCTGACTAGAATTGAGTTGAAGCTAAAGTGTTTTAGGTTTGGAAACATTCATATAAAGTAAGTTGAACAAAAAATTTGAGTGAAAAATAATTGTAGAATCAAAAGCTACAATTTCTAGCTTCAAGTAGAATCAATTCTAGAGGCATAATCAATTCTACTCTAGAGAAACCAAACATGTCAAAAACAATTCTGCACATCTAGAATCAATTATAACTCCTTCAAAAGTGGAACCAAACATACATTAGTGGATATGGCTTATACGTCTCAATCCCTGAATCCCCGCAACCAGTACCACCACATGGCTGGCCCAAGGGTAAGGCCACTAAAGCCCTCACTTTAAGCCCAATAACAAAATATTATTTTTCATTAGATTTTACAAGCAAAACGGCCTCATATTCTAACAAAAAAAGGCCTTGTATATTCAACCTTGCTTTAGGTTCCACTTATAGACTATTAAGGAAAAATTATTGGGTTGGCCCTGCACCACCAAGCCACGTTATGACTCCCTCCAGTCATACAATTTCAAAAATCAACTTAGATGTTTTATCTATCAACTATTGATATGAATTAATGACTGATATTATATTTTGATGCGTAACTAATCATAATTGTGTTGAATTTCTAGAACTTGACCAACATGCACATTGGCCCATAATTTTTTCACTTTGTTATTTACTTAAGAGAAGCTAAATTTTAGAATTTGACCAATCTTATGTGAGCAATTCACTTTAGAGACAGGAAAAGAAGTCATCCTAAGTTCTTAGCTGTTGATGAATAGGTCAAAGTTTGATATTTCAATATTTTCTTTTCATGGGATTAAGGGATATGCACCGTAAGTGTAAAGTTTTTACATTAACATCCAATCACAATTCTGTAATTTGCCATATCATATCATACATTTTTAAATAAAAGTTTTTACATGTTTATTATCTTGGCGAGTTATGATCGGATTCAGTGCAAAACAAATTTACGTTGACAGTATTTCCCTTAATCACTTTTTTAATAATGTATGTTTGACATCAATAATTGATTTATGCTTCAAAGATTACTTTATCATCTAGTCTAGAGTGAATTGCAGTTTTAGAGGAGTTAAATGCTACATACAAGTAGAACATATGCATGTAAGCAGTTATGCAAATGTATTCTTTCTTTCTCATTTTCTGTTCAAGAGATTCCTCTCCCCATTTCATTAAGAATGTTTGAATCCTTACATTGCATCTTTGATTTGTCTTTGTGCAGCAAAAAGGGTTTTCTGTAATACTTTCAGACATGTGTCCGTTGGTTTCTGGAATCACGACTAAAGATGCAGCTTTGTCATTTGAGCTAGGGATGCGAGCGCTAGACTTGGCTGTTGGCAAGGTTGCTTCTGTTTGCAATTCTGATGGTGATAATCTGGACGAGGTCAAGGAGCGATGTGATCCGTCTGGTTCAGATGATGGGGGATTGTTAAGAGTTGGTGGCCATCTTGTAGTCAAGCTTTTGGAGAGTGAAGATGCCAAAGGTGGTTTCATTTTTTGTCGAATAAAAACAAGCGTATGTCCATTGATTTGGTTGCGTATATGTTATTTAATAAATTTTATTTTAATATCTTTGCTGCAGAAATCAACCAGATTTGTAAACCATTGTTTACAAAGTCGGTGTGGCTGAGACCTAAAGCCACAAGACCTTCGTCGAGAGAGATCTATTTGATATGCCAAGGTTTAAAGCCAGAAATAAAGATATAGGTTGTTTTATGAGGTATGTCCTGGCGATGGTTTTTTACTCTATAATGCAGTTTTGTCAGATAATGGCTATAGCAGTGTCATAGCACTATACTGTAGCAGCTATAGCAGGTTGGCATAGAAAAATTTAAACAACCCCCTATTTTTCGCAATCTAGGATTGACCACACTGCTGTAATGTGAAACATGTAATTAGGAAATTCATGAGTTTACATCCAAGTTTTGATGCTAACATTATTCTAGAACTCTTACTTTCCACTGCCTTTTGATTTGTAAAGGATTGTAAGAGATTATGTATTAACTTGAAACAAAGAGAAAAAAAGGATTTTTGGGATTTGCATCCATAGATTTCTTTTGCATTTTTTCGTTACTTTCTATGTTTTGATTTGCATTCTCCATCCCATATGTTTTTTCTTGCAAATTCTGTTACTCGATCGTAAATTCAACCAAACAGAGAGATAGGTTAGTTTCATGTTATTTTAAGGATTCTGGATTCTCAATTTTGGTTATGTTTTTCCCATTTTTCAATGTTTTTTATGTAATTGGGTACCCATGATGGAAAGATTTGGAATTTGAGATCCGATCGTGGAAGTTAGGCTATGTTTGTTTTGAAACTGGTTTTTCTATTATAAAGTTTTGATTTTTATTTTTTCCTGGGGTGTTGAATTTTGAGGTTTTTTCTTCATGTTGTTAATTTGCCTTGAAATTCAAGGGTTTAGTGTGTACTTTACTAACTTTAAGATCATAATTGAGATGTTTAGTGTTTTTGATATTTTGTCTTGCAAATTAATTAATGTGCCTCTACTTTTTGTCTTGCAAATTAATTAATGTGCATCTACTTTATGCAGCTTTATAATTCTTTGTACTTTTGCTTGTGTTGACTTTTTTTAATCATATGGTTCATCTATTAGTTGATTTGTCTCAAATTTATTTTAATTAATTAGTTTAAAATTGGCTCATTATGTACTGATTTTGGATGGAAAATCTGATTTGTTTCCTTGTAATTCATCTCAACCTGTGTTAGGATAGTGCTTCTTTTTGATCAATTTGCCGATTTCTCTATTTGCTAAGATTTTGGTTACATTTAAGTTTTGATGTTATTCTTGCTTGTATTACTCGTATTATCGAAAAAAATTATGTGGATGATTGGTCTGCCAATTTGTGATAATCGACTTGATATATGAATCTTCAATTTTTATGACAGAGATAGATAGGATCTGATACATTCGTGCTTTTGATGAGTTGATGAGTGATAAATGTTCATGCTATGGTGTTGAAGAAGAGACTTGGTAATGGATTCAGGGACTATCAGGTCCCTAAGGTACCTAGATGTCCAAGATCTGTAAGAGTAAGTAATTGCCGCAATTCTTGCAATTGATTTTTACCTGTTTTTGTTTGGATGGTGTTTTACTTGAGTTTGAAATTCTTCAGAGGACGGTTGCGTTCAAGAAACCAGTCGAGGATGGCCACTCTTGTGCTTTTGAACTACTTGCATCTTTAGCCGGTGAGTTGCTGAAGGAGAGTGAAAGTTCCACCTCTAGCAATGCATCTGATGTTAAACCGTTAACAGAAGAGCGATTTCGTAATGGAAGTTGTGAAGAAAAGAACAGTGAGAAACATCTTGAGAGTGCTGGGACTGATTGTATACTAAAATGTATTTCTGCCAACAATAATGTGAAGTCTGAAATTTTCAAATTGGAGAATAGGTTTGGAAATCATTCTAATACTAATAGAATAGTAGAAGTTTCAAACCATCACGAGGCTGACAGTTCAGGCTTCAAGCGATTATCTGCTGTTCATTCTCCTTCTTTAGCCAACTTGAGCGGTAACATTAAATCCCCATTTTGTGGGGAATTGTTTCCCAATGCTTCCTTTTCAAGGAATGGGAGGAATGCTAGTAAGTTAGGTTTTATTGATGATGACGAAAACTTTATTAGGTGCAACAAAGTTTGCACCAAACCGAAGGCTTTTAGACCTTCACGATACATTGCACGCAAAATAATTAGGAAGCGGTGGACTTCCAATTCCAAACACTGGAAGGTTGCTCCAAAGTTGAAAGATTGTGAACATTCTCGCTATGGTAAGGTGTTTAGCAAATTGTCTGCAATGTCATGCTTTGTTATCTTTCACATATATGTCTATGAGTTAGGCTGTTCTTTTTTTGGACTAAACTTAAATGCAGTACCGCAAGTCCTGTTCTTACCGTTCCTCTCACCAAGACAGCTTTTCTTTGCACCCTTTTAGTTGGTGACAATTTTACTATCAGTGTCTAGAACATTGTTCCCAAGTTTTTTCCTTTTTTTTTTTTAACTGAAAAGAATGTGTAATTTGTAGATAATGGAATGCGACCCTCGAGTCATAAGAGGAAGACTTATTACAATTTCGAAAGATCTCAGTGTCACACTCTGTTGAAGAGGAAGAAATTATCAGACAGGGGTTCGATGCTAACTCATGGTGGAGGATTCAGCAGTGAGAGTGTATCCAATTCCCCTAAGAAAGTAATTGGAGGAAATAACCGTGGCTCATCTGCAAAAGTTCGCGTTTCTAAGGATTCTAATGGTAATTTATTGCATGGAGTTCATTAGTTTTGGTTAGCATATGTTCTTTGCATCCTACTGGCATTGATGATTTGTCCTTGCGTACAGTAAAGTTTCGCATCGAGTCATTCAGGATTCCAGAACTTTACGTCGACGTTCCCGAAACTGCAACTGTTGCTTCATTAAAGGTATGATGATTGAATGGTTTTAATTTTATTTTATTATATTAAGTTTAATATGTTTACTACTGTTTTCATTTAGTTTCATCAATAGTTTGGAGGGGTTGCAATGCGATGCATTATTTTCATCACTTTATTTTTATTGTTTAACTTTTTTATAGAGGACAGTTATGGAGGCGGTGATGACTATAATAGGAGGTGGAGTGCATGTTGGTGTTCTTGTTAAGGGGAAAAAAGTTGGAGACGATAACAGAACTCTTCGTCAGACTGGTATATCTTGTAAAGAAAATATAGATAAACTCGGTTTCGTGTTGGAGCCTAGCTCTTCTCAAGCTTCTCCAGTTGTTTGTGCTGGTGATCCTTCTCACCATGTAGCATCTCAGCCTACCAGGTGAGCGAAAGTTGAACAGTTTCTCTTAGCTTAGACTCTGTTTGGATAAACAACATAATTATGCGCATATAACACAAGTGTTTATCTTATAAGTGTTTATGTTTGGATAAACAACATAATTATGCGCATATAACATAAGTGTTTATCTTATAAGTGTTTATGTATAATCTTTTTCTATAACTAAAGATAAAACAAATTCCAACTGTTTTAATATAAGCTATAAGTTGTTTTTATAAGCTATCATGGAGAGCTGATGAAAAAAGCTGAGAACAAAATCACAAATTATAGACATGTCATAAACAGTCTCACAAAGTAACAAGTACTTATGCTAGTACATGAACTCAAATAAGGCAATTCGAACAGGCCTTTAATGGCAATAAACTAAGTGGCTTGTGGTTTTGGTCAGTGTTCATCCTGAAGAAATTTGATTACGGTTCAGCTCCATTCAATTGAATAATTCTTATATCTTTATCTAATTTTTGCAATTGGATTTGTCTAGGTCTCTAGGAACTCCCTCCATAGATTCAGGGATTTCTGTTGCCAAACAGGGTTCCTCCTTGCTTACAAATACTGGCGGTGACCTTGTTGACATGAACCATGAATCAGCTTCTTCCCTTGCTGACACTATATCTGATAAACTAACACAAGATTCCGGAGCGCTAGTTTCTGTTCCAGCTCATAGTACTGAGGCATTAACTGTTGTTCCCGTGAATCAGAACACTGGACCATCCGAGCTTGTACAGCGTCGAACCAGGAGACCATTTTCTGTGTCTGAAGTTGAAGCACTTGTTCATGCAGTTGAGGAAGTTGGAACTGGAAGGTAATATGGTTATCAGCTTCACCGTCTTTTTAGGCAGCGAAAGTGGGTTGCTGACATTTTTTTAAACTATGCAGGTGGCGTGATGTAAAGTTGCGATGTTTTGAGAATGCAGACCATAGGACTTATGTAGACTTGAAGGTACTCCCTTCTATCATATCTTAATTCTTTTGACTGAAACAGTTATTTACTTTACATTGTAAATTTAAAGAGAAAGATGAGGTAACACCTATAATGGAAAAGTGGTACAATATTGCCTTTAGAGGTTTTGACGGGACATTTTAGTTTAAGGTTGGATTCACTTTTGTTACAGGTAAAAATGAATTTAGAAACAAATGATTTTGATCATTATTTGACATGCTTAGGTCTTCTCAAGCAGAAATGCTTTTATTGCCAAATCTAACTTAAAACTAAGACTTATAGTTTCTATGAAAAGAAAACAATTCTAGCCTTAAATTTATTGTTCTTTCACGTCAATGTATCTAAACATCCATAACATTTTCCTTATTTTGATCGTAGTTGCATTGCATTTTTACAAAGTTTGTCGCATTTTGTATTGAATATCTTGTAGAATAAATAGAATTATGCAGTTGTTTTTGGTTATCACATCATTAGCTATGTTGTAATTTTTTTTTTTATCATAAATATCAGACTCGGTACTTGCGGTTTACAACATTCACACACCCTGCACACCGGCCATTAGCTATACGTTGTAATGGTAACATTGTTTAATTCCTAATGACTTGAACTTTTGGTTTGTGTGCAGGATAAATGGAAAACTTTAGTGCACACTGCAAAAATCTCTCCTCAACAAAGGAGAGGACAGCCAGTTCCTCAGGAGCTATTGGATAGGGTTTTGGCTGCACATGCTTATTGGTCTATCCACCAATCCAAGCAGCATGTCAAGCATCAAACCAATGTTGTCCAACCACTTGTGATTATGTAG
Protein sequence

>MS.gene32551.t1

MAKSGAPDFFYKEAQRLGYVARSAFKLVQIQKQHKLIKPGSSVLDLGCAPGSWLQVACQSLGPFHNGGSVLGIDHKKVKVPTSHCDSRVKTVAADVMTLPKQQLRDLSPKQKGFSVILSDMCPLVSGITTKDAALSFELGMRALDLAVGKVASVCNSDGDNLDEVKERCDPSGSDDGGLLRVGGHLVVKLLESEDAKEINQICKPLFTKSVWLRPKATRPSSREIYLICQVLSDNGYSSVIALYCSSYSRDYQVPKVPRCPRSVRRTVAFKKPVEDGHSCAFELLASLAGELLKESESSTSSNASDVKPLTEERFRNGSCEEKNSEKHLESAGTDCILKCISANNNVKSEIFKLENRFGNHSNTNRIVEVSNHHEADSSGFKRLSAVHSPSLANLSGNIKSPFCGELFPNASFSRNGRNASKLGFIDDDENFIRCNKVCTKPKAFRPSRYIARKIIRKRWTSNSKHWKVAPKLKDCEHSRYDNGMRPSSHKRKTYYNFERSQCHTLLKRKKLSDRGSMLTHGGGFSSESVSNSPKKVIGGNNRGSSAKVRVSKDSNVKFRIESFRIPELYVDVPETATVASLKRTVMEAVMTIIGGGVHVGVLVKGKKVGDDNRTLRQTGISCKENIDKLGFVLEPSSSQASPVVCAGDPSHHVASQPTRSLGTPSIDSGISVAKQGSSLLTNTGGDLVDMNHESASSLADTISDKLTQDSGALVSVPAHSTEALTVVPVNQNTGPSELVQRRTRRPFSVSEVEALVHAVEEVGTGRWRDVKLRCFENADHRTYVDLKDKWKTLVHTAKISPQQRRGQPVPQELLDRVLAAHAYWSIHQSKQHVKHQTNVVQPLVIM