Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40366.t1 | XP_003603563.1 | 97.7 | 393 | 8 | 1 | 1 | 393 | 1 | 392 | 5.50E-232 | 813.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40366.t1 | Q8VZQ0 | 60.4 | 376 | 149 | 0 | 11 | 386 | 11 | 386 | 1.1e-140 | 501.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40366.t1 | G7J2U5 | 97.7 | 393 | 8 | 1 | 1 | 393 | 1 | 392 | 3.9e-232 | 813.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene40366 | 0.810077 | 1.38E-50 | -1.69E-46 |
| MS.gene049283 | MS.gene40366 | 0.801886 | 7.29E-49 | -1.69E-46 |
| MS.gene049424 | MS.gene40366 | 0.810166 | 1.32E-50 | -1.69E-46 |
| MS.gene050105 | MS.gene40366 | 0.810257 | 1.26E-50 | -1.69E-46 |
| MS.gene051122 | MS.gene40366 | 0.835394 | 1.72E-56 | -1.69E-46 |
| MS.gene051368 | MS.gene40366 | 0.810336 | 1.21E-50 | -1.69E-46 |
| MS.gene051987 | MS.gene40366 | 0.807152 | 5.81E-50 | -1.69E-46 |
| MS.gene052500 | MS.gene40366 | 0.82174 | 3.44E-53 | -1.69E-46 |
| MS.gene052502 | MS.gene40366 | 0.829239 | 5.75E-55 | -1.69E-46 |
| MS.gene052674 | MS.gene40366 | 0.809467 | 1.87E-50 | -1.69E-46 |
| MS.gene052675 | MS.gene40366 | 0.806599 | 7.61E-50 | -1.69E-46 |
| MS.gene052677 | MS.gene40366 | 0.810075 | 1.38E-50 | -1.69E-46 |
| MS.gene052824 | MS.gene40366 | 0.8077 | 4.45E-50 | -1.69E-46 |
| MS.gene052826 | MS.gene40366 | 0.822018 | 2.96E-53 | -1.69E-46 |
| MS.gene052954 | MS.gene40366 | 0.829365 | 5.36E-55 | -1.69E-46 |
| MS.gene053575 | MS.gene40366 | 0.809064 | 2.28E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene40366 | 0.834393 | 3.08E-56 | -1.69E-46 |
| MS.gene054479 | MS.gene40366 | 0.82466 | 7.16E-54 | -1.69E-46 |
| MS.gene054892 | MS.gene40366 | 0.804906 | 1.72E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene40366 | 0.809569 | 1.77E-50 | -1.69E-46 |
| MS.gene055147 | MS.gene40366 | 0.80259 | 5.22E-49 | -1.69E-46 |
| MS.gene055225 | MS.gene40366 | 0.809326 | 2.00E-50 | -1.69E-46 |
| MS.gene055226 | MS.gene40366 | 0.812513 | 4.08E-51 | -1.69E-46 |
| MS.gene055701 | MS.gene40366 | 0.811239 | 7.73E-51 | -1.69E-46 |
| MS.gene056849 | MS.gene40366 | 0.805609 | 1.23E-49 | -1.69E-46 |
| MS.gene058429 | MS.gene40366 | 0.805476 | 1.31E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene40366 | 0.805633 | 1.21E-49 | -1.69E-46 |
| MS.gene060412 | MS.gene40366 | 0.800148 | 1.65E-48 | -1.69E-46 |
| MS.gene060840 | MS.gene40366 | 0.827531 | 1.49E-54 | -1.69E-46 |
| MS.gene060850 | MS.gene40366 | 0.820767 | 5.76E-53 | -1.69E-46 |
| MS.gene060851 | MS.gene40366 | 0.832615 | 8.55E-56 | -1.69E-46 |
| MS.gene060852 | MS.gene40366 | 0.832654 | 8.36E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40366.t1 | MTR_3g109120 | 97.710 | 393 | 8 | 1 | 1 | 393 | 1 | 392 | 0.0 | 792 |
| MS.gene40366.t1 | MTR_8g064810 | 39.452 | 365 | 220 | 1 | 20 | 383 | 175 | 539 | 1.87e-98 | 304 |
| MS.gene40366.t1 | MTR_8g064810 | 36.474 | 329 | 204 | 5 | 14 | 339 | 9 | 335 | 3.66e-66 | 220 |
| MS.gene40366.t1 | MTR_3g114530 | 36.935 | 398 | 232 | 6 | 12 | 390 | 32 | 429 | 1.56e-82 | 259 |
| MS.gene40366.t1 | MTR_8g064840 | 37.500 | 312 | 194 | 1 | 27 | 337 | 182 | 493 | 1.72e-75 | 243 |
| MS.gene40366.t1 | MTR_8g064840 | 32.979 | 376 | 240 | 7 | 14 | 385 | 9 | 376 | 4.46e-65 | 216 |
| MS.gene40366.t1 | MTR_8g064840 | 36.538 | 156 | 97 | 2 | 20 | 173 | 335 | 490 | 1.50e-28 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40366.t1 | AT4G31240 | 60.372 | 376 | 149 | 0 | 11 | 386 | 11 | 386 | 6.22e-174 | 491 |
| MS.gene40366.t1 | AT4G31240 | 60.372 | 376 | 149 | 0 | 11 | 386 | 11 | 386 | 6.22e-174 | 491 |
| MS.gene40366.t1 | AT1G60420 | 37.432 | 366 | 226 | 3 | 20 | 383 | 179 | 543 | 5.43e-94 | 293 |
| MS.gene40366.t1 | AT1G60420 | 36.975 | 357 | 215 | 6 | 13 | 363 | 12 | 364 | 2.50e-68 | 226 |
Find 0 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAAAAAAATTCCAGCTT+TGG | + | 24209:22615-22634 | MS.gene40366:CDS | 20.0% |
| !!! | TATTCAATTTTGCATCAAAT+GGG | - | 24209:21891-21910 | None:intergenic | 20.0% |
| ! | ACATGTACTTAAATTGGATA+TGG | + | 24209:22807-22826 | MS.gene40366:CDS | 25.0% |
| ! | AGTAAACAAAAACTTCAAGT+AGG | - | 24209:22174-22193 | None:intergenic | 25.0% |
| ! | GTAAACAAAAACTTCAAGTA+GGG | - | 24209:22173-22192 | None:intergenic | 25.0% |
| ! | TCACAAAACTAAGCAAATTA+TGG | - | 24209:21611-21630 | None:intergenic | 25.0% |
| ! | TGTTGACATTGAATTCTTTA+AGG | - | 24209:22525-22544 | None:intergenic | 25.0% |
| ! | TTATATCAAAGATTCTACAG+AGG | - | 24209:22600-22619 | None:intergenic | 25.0% |
| !! | AACATAATGGAATGAATGAT+GGG | - | 24209:21948-21967 | None:intergenic | 25.0% |
| !! | CTATTCAATTTTGCATCAAA+TGG | - | 24209:21892-21911 | None:intergenic | 25.0% |
| !! | GTAATCACGATTTTAAATTG+TGG | + | 24209:22202-22221 | MS.gene40366:intron | 25.0% |
| !!! | TGTTTTAGTTTCAACAGATA+GGG | + | 24209:22498-22517 | MS.gene40366:CDS | 25.0% |
| !!! | TTGTTTTAGTTTCAACAGAT+AGG | + | 24209:22497-22516 | MS.gene40366:CDS | 25.0% |
| AAAACGCGAAGAAGTAAATT+TGG | + | 24209:22080-22099 | MS.gene40366:CDS | 30.0% | |
| AACAATCTGAAGGATACAAA+AGG | + | 24209:22460-22479 | MS.gene40366:CDS | 30.0% | |
| AAGACTGATTGATAGATATC+GGG | + | 24209:21912-21931 | MS.gene40366:CDS | 30.0% | |
| AGAATCACATACATATGCTT+TGG | - | 24209:22833-22852 | None:intergenic | 30.0% | |
| CATCTTTGAATCATGAGATT+AGG | + | 24209:21657-21676 | MS.gene40366:intron | 30.0% | |
| CTAATCTCATGATTCAAAGA+TGG | - | 24209:21659-21678 | None:intergenic | 30.0% | |
| GAAGACTGATTGATAGATAT+CGG | + | 24209:21911-21930 | MS.gene40366:CDS | 30.0% | |
| GCATGAACATGTACTTAAAT+TGG | + | 24209:22801-22820 | MS.gene40366:CDS | 30.0% | |
| TCATAAAGATCAACAAGATG+AGG | - | 24209:21763-21782 | None:intergenic | 30.0% | |
| TGTGATTCTTGTAAGAAACA+AGG | + | 24209:22844-22863 | MS.gene40366:CDS | 30.0% | |
| TTGACAAAAACATGATTGAG+TGG | + | 24209:21983-22002 | MS.gene40366:CDS | 30.0% | |
| TTGGATGAATTATTGACACA+TGG | + | 24209:22099-22118 | MS.gene40366:CDS | 30.0% | |
| ! | AAACTAATGACTTTTCCATC+TGG | - | 24209:22652-22671 | None:intergenic | 30.0% |
| ! | ACGATTTTAAATTGTGGTTG+CGG | + | 24209:22208-22227 | MS.gene40366:intron | 30.0% |
| ! | AGTTCCTTTTATCTTGTGAA+GGG | + | 24209:21432-21451 | MS.gene40366:CDS | 30.0% |
| ! | GAACATAATGGAATGAATGA+TGG | - | 24209:21949-21968 | None:intergenic | 30.0% |
| ! | GGAAAAGTCATTAGTTTGAA+TGG | + | 24209:22655-22674 | MS.gene40366:CDS | 30.0% |
| ! | TTCGCGTTTTCTTTTATCTA+TGG | - | 24209:22070-22089 | None:intergenic | 30.0% |
| !! | CATGTTTTTGTCAACAGTTA+AGG | - | 24209:21977-21996 | None:intergenic | 30.0% |
| !!! | AAAACTGTTGGGCTATTTTT+CGG | + | 24209:22382-22401 | MS.gene40366:CDS | 30.0% |
| !!! | TTTGCTTAGTTTTGTGATCT+TGG | + | 24209:21614-21633 | MS.gene40366:intron | 30.0% |
| AGATGCATACAACAATCTGA+AGG | + | 24209:22450-22469 | MS.gene40366:CDS | 35.0% | |
| ATGAAACACTGAGAAAGAGA+GGG | + | 24209:21779-21798 | MS.gene40366:CDS | 35.0% | |
| CATTTCAGGAGATGATAGAA+AGG | + | 24209:22137-22156 | MS.gene40366:CDS | 35.0% | |
| CTAAAACAATCTCAAAGCAG+TGG | - | 24209:22486-22505 | None:intergenic | 35.0% | |
| CTTATCCTCGATTCAGTAAA+TGG | - | 24209:22718-22737 | None:intergenic | 35.0% | |
| GAATTCCATAAATGCACTCT+AGG | - | 24209:22294-22313 | None:intergenic | 35.0% | |
| GAGGATAAGAGATTTAGAAG+CGG | + | 24209:22729-22748 | MS.gene40366:CDS | 35.0% | |
| GATGAATTATTGACACATGG+AGG | + | 24209:22102-22121 | MS.gene40366:CDS | 35.0% | |
| TATGAAACACTGAGAAAGAG+AGG | + | 24209:21778-21797 | MS.gene40366:CDS | 35.0% | |
| ! | CTTTTCCATTTACTGAATCG+AGG | + | 24209:22710-22729 | MS.gene40366:CDS | 35.0% |
| ! | GAGTTCCTTTTATCTTGTGA+AGG | + | 24209:21431-21450 | MS.gene40366:CDS | 35.0% |
| ! | GTTAAGGCATCAGAACATAA+TGG | - | 24209:21961-21980 | None:intergenic | 35.0% |
| !! | AAACATGATTGAGTGGATTG+AGG | + | 24209:21990-22009 | MS.gene40366:CDS | 35.0% |
| !! | ATTGAGTGGATTGAGGATTA+TGG | + | 24209:21997-22016 | MS.gene40366:CDS | 35.0% |
| !!! | ATTCCAGCTTTGGTTTTCAT+TGG | + | 24209:22625-22644 | MS.gene40366:CDS | 35.0% |
| AGGTCAACAATGACCAATGT+TGG | + | 24209:22929-22948 | MS.gene40366:CDS | 40.0% | |
| ATGTTTCATCGTTGCATTGC+AGG | + | 24209:22336-22355 | MS.gene40366:intron | 40.0% | |
| ATTGTGGTTGCGGTCAAATA+TGG | + | 24209:22218-22237 | MS.gene40366:intron | 40.0% | |
| CAAGTCCTAGAGTGCATTTA+TGG | + | 24209:22286-22305 | MS.gene40366:intron | 40.0% | |
| CAGTTCATGATCTCCTCATA+CGG | + | 24209:22679-22698 | MS.gene40366:CDS | 40.0% | |
| CCTTCCCTTCACAAGATAAA+AGG | - | 24209:21439-21458 | None:intergenic | 40.0% | |
| GTAAGAAACAAGGCAAGTTC+TGG | + | 24209:22854-22873 | MS.gene40366:CDS | 40.0% | |
| TACCCTTGTCTGATTGCAAT+GGG | + | 24209:21680-21699 | MS.gene40366:CDS | 40.0% | |
| TCATCCTAGTTGTCTTGAGA+AGG | + | 24209:22909-22928 | MS.gene40366:CDS | 40.0% | |
| TCATGTCTCTTCCTTGTGAA+AGG | - | 24209:22036-22055 | None:intergenic | 40.0% | |
| TCCCATATGAAGACAGAACA+AGG | + | 24209:22569-22588 | MS.gene40366:CDS | 40.0% | |
| TGAGGAATGAAAAGTCTGCA+AGG | - | 24209:21745-21764 | None:intergenic | 40.0% | |
| TTCCCATTGCAATCAGACAA+GGG | - | 24209:21685-21704 | None:intergenic | 40.0% | |
| TTGACCTTCTCAAGACAACT+AGG | - | 24209:22916-22935 | None:intergenic | 40.0% | |
| TTGATATCCTCAACCTGTTG+AGG | - | 24209:22781-22800 | None:intergenic | 40.0% | |
| TTTCACAAGGAAGAGACATG+AGG | + | 24209:22035-22054 | MS.gene40366:CDS | 40.0% | |
| TTTCCCATTGCAATCAGACA+AGG | - | 24209:21686-21705 | None:intergenic | 40.0% | |
| ! | AAATAGCCCAACAGTTTTCC+CGG | - | 24209:22380-22399 | None:intergenic | 40.0% |
| ! | ATCTGCAAGTTGAATGGTGA+AGG | - | 24209:22434-22453 | None:intergenic | 40.0% |
| ! | CCTTTTATCTTGTGAAGGGA+AGG | + | 24209:21436-21455 | MS.gene40366:CDS | 40.0% |
| ! | GCCTTGTTCTGTCTTCATAT+GGG | - | 24209:22573-22592 | None:intergenic | 40.0% |
| ! | GTATGCATCTGCAAGTTGAA+TGG | - | 24209:22440-22459 | None:intergenic | 40.0% |
| ! | TGACAGCTTTGATGTTCTGA+AGG | + | 24209:21388-21407 | MS.gene40366:CDS | 40.0% |
| ! | TGCCTTGTTCTGTCTTCATA+TGG | - | 24209:22574-22593 | None:intergenic | 40.0% |
| !! | CAGATTTTAGTGTCGCAACA+GGG | - | 24209:21571-21590 | None:intergenic | 40.0% |
| !! | GTCTATTTTTCTCTGCCAAC+TGG | + | 24209:21713-21732 | MS.gene40366:CDS | 40.0% |
| !! | TTGGTTTTCATTGGACCAGA+TGG | + | 24209:22634-22653 | MS.gene40366:CDS | 40.0% |
| AAAAGCTTCTGCTCCGTATG+AGG | - | 24209:22695-22714 | None:intergenic | 45.0% | |
| AACTAGCCGGGAAAACTGTT+GGG | + | 24209:22371-22390 | MS.gene40366:CDS | 45.0% | |
| CCTGTTGCGACACTAAAATC+TGG | + | 24209:21569-21588 | MS.gene40366:intron | 45.0% | |
| CTGATGCGTTTCCTTTCACA+AGG | + | 24209:22022-22041 | MS.gene40366:CDS | 45.0% | |
| CTTTAAACCCATCCTCGTCA+TGG | - | 24209:21837-21856 | None:intergenic | 45.0% | |
| GGACGCAACTTTCTCATTTC+AGG | + | 24209:22123-22142 | MS.gene40366:CDS | 45.0% | |
| GGTCCAATGAAAACCAAAGC+TGG | - | 24209:22631-22650 | None:intergenic | 45.0% | |
| GTACCCTTGTCTGATTGCAA+TGG | + | 24209:21679-21698 | MS.gene40366:CDS | 45.0% | |
| GTGCTTGTATCTGAACTAGC+CGG | + | 24209:22358-22377 | MS.gene40366:CDS | 45.0% | |
| TCAACAGAACTAGCATGCCT+TGG | + | 24209:22539-22558 | MS.gene40366:CDS | 45.0% | |
| TCATATGGGATAGCAAGCCA+AGG | - | 24209:22559-22578 | None:intergenic | 45.0% | |
| TGCTTGTATCTGAACTAGCC+GGG | + | 24209:22359-22378 | MS.gene40366:CDS | 45.0% | |
| ! | AACACATCAAGAGTATGCCG+TGG | + | 24209:21857-21876 | MS.gene40366:CDS | 45.0% |
| ! | CTGAAGGTTTTTGCAGCTGA+AGG | + | 24209:21404-21423 | MS.gene40366:CDS | 45.0% |
| ! | TGAATGGTGAAGGCATGACA+AGG | - | 24209:22424-22443 | None:intergenic | 45.0% |
| !! | CACTCTAGGACTTGACATCA+AGG | - | 24209:22280-22299 | None:intergenic | 45.0% |
| !! | CCAGATTTTAGTGTCGCAAC+AGG | - | 24209:21572-21591 | None:intergenic | 45.0% |
| AAGCCTCTCCTTCCTTTCTC+AGG | - | 24209:22756-22775 | None:intergenic | 50.0% | |
| AGCCTCTCCTTCCTTTCTCA+GGG | - | 24209:22755-22774 | None:intergenic | 50.0% | |
| ATGACAAGGAGGAGACCAGT+AGG | - | 24209:22410-22429 | None:intergenic | 50.0% | |
| CATCTCCTTTGACCATGACG+AGG | + | 24209:21822-21841 | MS.gene40366:CDS | 50.0% | |
| CCTTTGACCATGACGAGGAT+GGG | + | 24209:21827-21846 | MS.gene40366:CDS | 50.0% | |
| CGGTCAAATATGGCTGATGC+AGG | + | 24209:22228-22247 | MS.gene40366:intron | 50.0% | |
| GAACTAGCCGGGAAAACTGT+TGG | + | 24209:22370-22389 | MS.gene40366:CDS | 50.0% | |
| TCAAATGGGACAGCTAGCCA+CGG | - | 24209:21877-21896 | None:intergenic | 50.0% | |
| TCCTTTGACCATGACGAGGA+TGG | + | 24209:21826-21845 | MS.gene40366:CDS | 50.0% | |
| TCTGCAAGGTCTACACCAGT+TGG | - | 24209:21731-21750 | None:intergenic | 50.0% | |
| TTTAGAAGCGGCCCTGAGAA+AGG | + | 24209:22741-22760 | MS.gene40366:CDS | 50.0% | |
| ! | ATGGTGAAGGCATGACAAGG+AGG | - | 24209:22421-22440 | None:intergenic | 50.0% |
| !!! | GGCTATTTTTCGGTGCCTAC+TGG | + | 24209:22392-22411 | MS.gene40366:CDS | 50.0% |
| AGGAGAGGCTTTGCCTCAAC+AGG | + | 24209:22765-22784 | MS.gene40366:CDS | 55.0% | |
| CCCATCCTCGTCATGGTCAA+AGG | - | 24209:21830-21849 | None:intergenic | 55.0% | |
| GGCTTTGCCTCAACAGGTTG+AGG | + | 24209:22771-22790 | MS.gene40366:CDS | 55.0% | |
| ! | ATGCAGGCGTTGCTATTGCG+TGG | + | 24209:22244-22263 | MS.gene40366:intron | 55.0% |
| GAAGCGGCCCTGAGAAAGGA+AGG | + | 24209:22745-22764 | MS.gene40366:CDS | 60.0% | |
| GGCCCTGAGAAAGGAAGGAG+AGG | + | 24209:22750-22769 | MS.gene40366:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 24209 | gene | 21353 | 22954 | 21353 | ID=MS.gene40366 |
| 24209 | mRNA | 21353 | 22954 | 21353 | ID=MS.gene40366.t1;Parent=MS.gene40366 |
| 24209 | exon | 21353 | 21457 | 21353 | ID=MS.gene40366.t1.exon1;Parent=MS.gene40366.t1 |
| 24209 | CDS | 21353 | 21457 | 21353 | ID=cds.MS.gene40366.t1;Parent=MS.gene40366.t1 |
| 24209 | exon | 21679 | 22158 | 21679 | ID=MS.gene40366.t1.exon2;Parent=MS.gene40366.t1 |
| 24209 | CDS | 21679 | 22158 | 21679 | ID=cds.MS.gene40366.t1;Parent=MS.gene40366.t1 |
| 24209 | exon | 22358 | 22954 | 22358 | ID=MS.gene40366.t1.exon3;Parent=MS.gene40366.t1 |
| 24209 | CDS | 22358 | 22954 | 22358 | ID=cds.MS.gene40366.t1;Parent=MS.gene40366.t1 |
| Gene Sequence |
| Protein sequence |
