AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43906

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43906.t1 MTR_5g035080 95.418 371 17 0 3 373 63 433 0.0 750
MS.gene43906.t1 MTR_5g035150 71.978 364 93 4 7 364 75 435 0.0 538
MS.gene43906.t1 MTR_8g104970 67.697 356 113 2 7 361 112 466 0.0 523
MS.gene43906.t1 MTR_3g109840 67.507 357 113 3 6 362 64 417 0.0 509
MS.gene43906.t1 MTR_5g035090 65.840 363 115 4 6 364 74 431 2.31e-177 500
MS.gene43906.t1 MTR_5g035070 65.775 374 119 4 4 373 76 444 3.97e-174 492
MS.gene43906.t1 MTR_7g033230 64.426 357 125 2 7 362 256 611 5.73e-173 496
MS.gene43906.t1 MTR_7g033230 64.426 357 125 2 7 362 326 681 1.39e-171 494
MS.gene43906.t1 MTR_5g035140 68.702 262 80 2 102 362 7 267 4.42e-137 392
MS.gene43906.t1 MTR_8g099300 42.458 358 190 8 11 360 70 419 4.40e-97 295
MS.gene43906.t1 MTR_6g016880 40.113 354 194 9 15 361 78 420 1.00e-92 285
MS.gene43906.t1 MTR_8g027900 40.793 353 194 7 15 360 211 555 7.03e-92 286
MS.gene43906.t1 MTR_7g019120 38.136 354 201 9 15 361 79 421 1.07e-84 264
MS.gene43906.t1 MTR_6g016875 40.223 358 191 10 15 361 64 409 1.43e-83 261
MS.gene43906.t1 MTR_6g007777 37.465 355 204 9 15 362 116 459 7.24e-80 253
MS.gene43906.t1 MTR_2g016060 37.119 361 195 13 18 362 58 402 2.26e-75 239
MS.gene43906.t1 MTR_1g076690 35.278 360 211 10 15 362 223 572 1.84e-68 226
MS.gene43906.t1 MTR_6g061710 34.626 361 212 11 15 362 167 516 3.41e-65 216
MS.gene43906.t1 MTR_7g094750 32.976 373 196 17 15 362 91 434 3.40e-52 180
MS.gene43906.t1 MTR_0136s0010 33.787 367 200 14 15 362 170 512 1.37e-50 177
MS.gene43906.t1 MTR_2g022560 33.058 363 212 10 15 364 97 441 3.23e-50 174
MS.gene43906.t1 MTR_7g093450 33.333 378 207 14 15 371 248 601 1.02e-48 173
MS.gene43906.t1 MTR_8g069790 30.458 371 207 13 15 363 178 519 5.66e-45 162
MS.gene43906.t1 MTR_5g035120 65.686 102 35 0 4 105 64 165 6.44e-45 152
MS.gene43906.t1 MTR_8g093530 31.389 360 200 14 15 360 90 416 8.34e-45 160
MS.gene43906.t1 MTR_4g016940 30.053 376 221 13 4 360 75 427 1.45e-44 159
MS.gene43906.t1 MTR_4g126950 31.680 363 206 13 15 360 109 446 2.03e-44 159
MS.gene43906.t1 MTR_1g058380 30.628 382 212 17 5 362 45 397 4.11e-44 157
MS.gene43906.t1 MTR_5g059400 30.513 390 213 17 11 371 168 528 5.20e-44 159
MS.gene43906.t1 MTR_1g116130 30.513 390 213 17 11 371 168 528 5.20e-44 159
MS.gene43906.t1 MTR_5g033800 29.919 371 218 13 9 362 140 485 3.90e-43 156
MS.gene43906.t1 MTR_3g464570 31.233 365 209 11 11 360 39 376 6.39e-43 153
MS.gene43906.t1 MTR_4g125060 28.883 367 213 10 15 360 132 471 6.84e-42 153
MS.gene43906.t1 MTR_4g125060 28.883 367 213 10 15 360 171 510 8.47e-42 153
MS.gene43906.t1 MTR_5g071350 29.315 365 216 11 11 356 54 395 1.01e-41 151
MS.gene43906.t1 MTR_4g125060 28.883 367 213 10 15 360 200 539 1.34e-41 153
MS.gene43906.t1 MTR_7g109550 30.362 359 203 13 15 357 137 464 2.08e-41 151
MS.gene43906.t1 MTR_8g463790 29.412 374 211 13 12 362 68 411 4.80e-41 149
MS.gene43906.t1 MTR_3g070900 28.809 361 223 13 12 356 103 445 7.12e-40 147
MS.gene43906.t1 MTR_8g093870 28.191 376 204 14 15 360 133 472 8.26e-40 147
MS.gene43906.t1 MTR_4g121560 29.076 368 219 11 11 360 110 453 4.44e-39 145
MS.gene43906.t1 MTR_2g091175 31.549 355 188 15 15 356 50 362 8.54e-39 142
MS.gene43906.t1 MTR_7g068920 28.906 384 215 17 4 360 69 421 3.34e-38 142
MS.gene43906.t1 MTR_1g050448 28.451 355 213 10 15 360 53 375 3.97e-38 140
MS.gene43906.t1 MTR_3g090120 29.708 377 212 15 7 364 121 463 4.01e-38 142
MS.gene43906.t1 MTR_3g049330 30.000 350 203 11 15 356 31 346 1.16e-36 136
MS.gene43906.t1 MTR_3g021090 30.769 351 197 15 15 356 31 344 1.31e-36 137
MS.gene43906.t1 MTR_3g035830 30.337 356 192 15 15 356 31 344 2.82e-36 135
MS.gene43906.t1 MTR_3g021060 30.484 351 198 15 15 356 46 359 1.42e-35 134
MS.gene43906.t1 MTR_4g014440 29.050 358 222 15 15 356 62 403 2.75e-35 134
MS.gene43906.t1 MTR_7g094750 33.984 256 129 10 15 249 91 327 5.78e-35 131
MS.gene43906.t1 MTR_4g079700 30.899 356 197 15 15 360 47 363 7.32e-35 132
MS.gene43906.t1 MTR_7g070060 28.415 366 214 16 15 356 64 405 8.03e-35 132
MS.gene43906.t1 MTR_2g073510 29.775 356 211 14 12 360 30 353 2.67e-33 127
MS.gene43906.t1 MTR_5g034810 29.888 358 207 12 7 356 27 348 3.08e-33 127
MS.gene43906.t1 MTR_5g083260 27.500 360 229 13 12 357 80 421 1.94e-32 126
MS.gene43906.t1 MTR_3g071650 27.446 368 213 16 15 357 89 427 2.79e-32 126
MS.gene43906.t1 MTR_6g016240 27.665 394 213 17 1 360 61 416 4.27e-32 125
MS.gene43906.t1 MTR_2g073500 28.219 365 201 15 12 360 26 345 1.32e-31 122
MS.gene43906.t1 MTR_4g088030 26.016 369 219 11 1 356 19 346 2.25e-31 122
MS.gene43906.t1 MTR_4g087730 28.732 355 215 11 7 356 37 358 1.48e-30 120
MS.gene43906.t1 MTR_4g014430 26.790 377 219 16 9 360 102 446 2.21e-30 121
MS.gene43906.t1 MTR_2g015720 28.804 368 213 15 3 360 30 358 2.72e-30 119
MS.gene43906.t1 MTR_5g082880 26.503 366 222 13 15 356 165 507 2.77e-30 121
MS.gene43906.t1 MTR_7g019140 50.000 92 46 0 15 106 79 170 3.34e-29 111
MS.gene43906.t1 MTR_5g033800 32.487 197 117 5 9 191 140 334 1.84e-28 114
MS.gene43906.t1 MTR_1g112210 26.027 365 202 13 15 356 59 378 5.01e-28 113
MS.gene43906.t1 MTR_4g092830 26.064 376 217 12 15 360 48 392 6.08e-27 111
MS.gene43906.t1 MTR_1g112210 25.419 358 200 12 15 356 59 365 2.90e-26 108
MS.gene43906.t1 MTR_7g070060 36.090 133 79 2 15 141 64 196 3.17e-24 101
MS.gene43906.t1 MTR_3g464570 28.378 296 170 11 80 360 4 272 1.59e-21 93.6
MS.gene43906.t1 MTR_1g111010 44.898 98 52 2 8 103 25 122 1.30e-20 92.0
MS.gene43906.t1 MTR_7g117160 34.194 155 67 5 205 359 70 189 7.16e-19 84.0
MS.gene43906.t1 MTR_0929s0010 55.102 49 22 0 15 63 79 127 1.88e-13 67.0
MS.gene43906.t1 MTR_7g019130 31.818 132 78 6 129 255 1 125 1.81e-12 64.7
MS.gene43906.t1 MTR_4g102570 34.109 129 70 4 127 249 2 121 2.65e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43906.t1 AT1G70230 51.801 361 151 8 8 363 72 414 1.04e-132 386
MS.gene43906.t1 AT4G23790 42.105 361 192 10 10 360 74 427 1.62e-94 289
MS.gene43906.t1 AT1G01430 41.620 358 188 10 15 360 99 447 3.67e-94 289
MS.gene43906.t1 AT4G01080 42.000 350 185 9 15 358 91 428 5.12e-93 285
MS.gene43906.t1 AT4G11090 42.061 359 193 9 10 360 74 425 6.05e-91 280
MS.gene43906.t1 AT5G15890 40.503 358 189 10 11 361 183 523 1.67e-89 279
MS.gene43906.t1 AT3G02440 38.997 359 201 8 15 366 135 482 5.03e-88 274
MS.gene43906.t1 AT3G02440 38.997 359 201 8 15 366 127 474 5.39e-88 274
MS.gene43906.t1 AT3G28150 36.559 372 212 10 3 362 53 412 1.16e-83 261
MS.gene43906.t1 AT5G15900 37.017 362 207 11 15 367 67 416 1.24e-80 253
MS.gene43906.t1 AT5G15900 37.017 362 207 11 15 367 86 435 2.16e-80 253
MS.gene43906.t1 AT4G25360 33.880 366 216 9 12 362 172 526 3.12e-64 213
MS.gene43906.t1 AT4G25360 33.880 366 216 9 12 362 172 526 3.12e-64 213
MS.gene43906.t1 AT5G51640 32.099 405 211 14 2 371 123 498 1.04e-62 209
MS.gene43906.t1 AT5G19160 32.973 370 222 9 15 371 99 455 2.07e-55 189
MS.gene43906.t1 AT3G06080 30.997 371 207 12 15 362 103 447 4.52e-49 172
MS.gene43906.t1 AT5G06700 32.095 377 209 15 15 370 255 605 8.88e-49 174
MS.gene43906.t1 AT5G64020 30.831 373 210 12 15 360 57 408 5.65e-48 167
MS.gene43906.t1 AT3G11570 31.933 357 197 13 31 368 98 427 4.30e-47 166
MS.gene43906.t1 AT3G12060 31.565 377 211 15 15 370 196 546 7.78e-47 167
MS.gene43906.t1 AT2G30010 31.233 365 218 11 10 360 51 396 2.54e-46 163
MS.gene43906.t1 AT2G30010 31.421 366 216 13 10 360 94 439 3.87e-46 164
MS.gene43906.t1 AT1G48880 33.516 364 195 15 10 360 110 439 1.47e-45 162
MS.gene43906.t1 AT5G58600 30.252 357 216 10 15 360 66 400 2.84e-43 155
MS.gene43906.t1 AT2G14530 30.423 378 198 15 15 360 62 406 6.28e-43 154
MS.gene43906.t1 AT5G06230 31.536 371 202 13 15 360 21 364 6.67e-43 153
MS.gene43906.t1 AT5G20680 28.084 381 210 10 9 360 112 457 1.79e-42 154
MS.gene43906.t1 AT5G06230 31.536 371 202 13 15 360 62 405 1.81e-42 153
MS.gene43906.t1 AT5G20680 28.084 381 210 10 9 360 188 533 3.80e-42 154
MS.gene43906.t1 AT3G62390 29.839 372 212 16 9 362 134 474 4.05e-42 153
MS.gene43906.t1 AT5G20680 28.084 381 210 10 9 360 206 551 5.07e-42 154
MS.gene43906.t1 AT5G20680 28.084 381 210 10 9 360 206 551 5.07e-42 154
MS.gene43906.t1 AT5G20680 28.084 381 210 10 9 360 206 551 5.07e-42 154
MS.gene43906.t1 AT5G20680 28.084 381 210 10 9 360 206 551 5.07e-42 154
MS.gene43906.t1 AT5G49340 27.869 366 220 10 15 360 100 441 1.63e-41 151
MS.gene43906.t1 AT5G20590 31.044 364 208 14 15 360 141 479 2.07e-41 152
MS.gene43906.t1 AT2G40160 29.589 365 212 16 15 356 77 419 5.76e-40 147
MS.gene43906.t1 AT2G40160 29.589 365 212 16 15 356 77 419 6.30e-40 147
MS.gene43906.t1 AT2G42570 30.518 367 201 15 7 360 39 364 1.70e-39 144
MS.gene43906.t1 AT1G73140 28.421 380 222 15 2 360 55 405 1.73e-39 145
MS.gene43906.t1 AT2G37720 28.982 383 202 11 10 360 138 482 2.87e-39 146
MS.gene43906.t1 AT2G40150 29.006 362 221 14 15 356 71 416 3.41e-39 144
MS.gene43906.t1 AT1G60790 27.321 377 230 14 6 361 181 534 4.71e-39 146
MS.gene43906.t1 AT5G01360 28.261 368 233 13 4 357 25 375 6.65e-39 143
MS.gene43906.t1 AT3G55990 29.301 372 227 16 3 356 126 479 1.33e-38 144
MS.gene43906.t1 AT5G01360 28.261 368 233 13 4 357 81 431 2.46e-38 142
MS.gene43906.t1 AT5G19160 34.818 247 139 6 15 249 99 335 5.00e-37 137
MS.gene43906.t1 AT2G31110 29.428 367 201 16 5 356 35 358 1.54e-36 136
MS.gene43906.t1 AT2G30900 28.895 353 206 13 15 356 43 361 1.67e-35 133
MS.gene43906.t1 AT2G38320 28.686 373 226 16 4 356 48 400 1.73e-35 134
MS.gene43906.t1 AT3G11030 27.717 368 219 15 15 360 106 448 4.23e-35 134
MS.gene43906.t1 AT3G14850 30.833 360 196 16 15 360 34 354 2.95e-34 130
MS.gene43906.t1 AT2G40320 27.322 366 222 14 12 357 78 419 4.31e-34 130
MS.gene43906.t1 AT1G29050 28.261 368 216 15 3 360 49 378 6.88e-34 129
MS.gene43906.t1 AT5G64470 27.297 370 217 15 14 360 54 394 9.50e-33 127
MS.gene43906.t1 AT5G64470 26.862 376 217 16 14 360 54 400 1.30e-31 124
MS.gene43906.t1 AT3G54260 27.374 358 206 13 15 356 51 370 2.22e-31 122
MS.gene43906.t1 AT1G78710 29.700 367 210 15 4 360 28 356 2.72e-31 122
MS.gene43906.t1 AT3G06080 30.435 253 142 6 15 249 103 339 2.93e-31 121
MS.gene43906.t1 AT2G34070 27.298 359 208 15 15 360 65 383 1.35e-30 120
MS.gene43906.t1 AT3G62390 29.804 255 149 8 9 249 134 372 1.03e-29 119
MS.gene43906.t1 AT2G40160 29.876 241 157 8 15 249 77 311 2.71e-29 115
MS.gene43906.t1 AT5G01620 26.776 366 230 11 11 360 106 449 7.95e-29 116
MS.gene43906.t1 AT5G01620 26.776 366 230 11 11 360 106 449 7.95e-29 116
MS.gene43906.t1 AT2G38320 28.834 326 203 14 45 356 7 317 8.34e-29 114
MS.gene43906.t1 AT5G01620 26.776 366 230 11 11 360 114 457 1.06e-28 116
MS.gene43906.t1 AT5G58600 32.353 238 143 7 15 248 66 289 7.88e-28 111
MS.gene43906.t1 AT5G06230 31.621 253 145 7 15 249 62 304 1.65e-27 110
MS.gene43906.t1 AT5G20590 34.715 193 109 6 15 191 141 332 1.94e-27 112
MS.gene43906.t1 AT2G30900 36.628 172 92 6 15 179 43 204 4.54e-24 100
MS.gene43906.t1 AT2G31110 31.053 190 112 5 5 186 35 213 8.36e-24 99.8
MS.gene43906.t1 AT1G78710 37.126 167 98 5 4 166 21 184 5.58e-22 95.1
MS.gene43906.t1 AT5G64470 26.389 288 171 12 14 278 54 323 5.25e-20 90.1
MS.gene43906.t1 AT5G64470 26.882 279 163 12 14 269 54 314 4.57e-19 87.4

Find 85 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCTTAAAGCCTTTCTTAA+TGG 0.091385 5.4:-24955086 None:intergenic
TTCCATTAAGAAAGGCTTTA+AGG 0.121836 5.4:+24955084 MS.gene43906:CDS
GCCACCAATGTGAACCTTTA+TGG 0.250930 5.4:-24954785 None:intergenic
TGCTTAAATTGTTCCGGTTT+CGG 0.254274 5.4:+24955032 MS.gene43906:CDS
CAAACTACCTTTACTATAGA+TGG 0.262429 5.4:+24954604 MS.gene43906:CDS
CCTTTATGGTGGACCGGTTT+AGG 0.263456 5.4:-24954771 None:intergenic
GGTCTTAATTACACTGATAT+TGG 0.285156 5.4:+24955053 MS.gene43906:CDS
GTGACTACTTCAATGGAAAA+TGG 0.286087 5.4:+24954490 MS.gene43906:CDS
GGAAAGGGTGAGAAGGAAAT+TGG 0.307402 5.4:+24955242 MS.gene43906:CDS
TACAAAGAAGAGACTCAAAT+TGG 0.314351 5.4:-24954728 None:intergenic
TGTGTTCATTGGTGTTTGCC+TGG 0.324131 5.4:+24955464 MS.gene43906:CDS
CTTGGAAGATTGCACTCATT+TGG 0.324777 5.4:-24954630 None:intergenic
GACTTGATTGTGTTATCGTT+TGG 0.361764 5.4:+24954954 MS.gene43906:CDS
AAATGGATTCATGACAATAA+AGG 0.362706 5.4:+24954507 MS.gene43906:CDS
TGTTCAAAGACTGAACCTTA+TGG 0.369816 5.4:+24955221 MS.gene43906:CDS
TTAGGATGCTTAAATTGTTC+CGG 0.372597 5.4:+24955026 MS.gene43906:CDS
AGATGATGAAGAAGTGGGAT+TGG 0.380315 5.4:+24955519 MS.gene43906:CDS
TCATAACGCCTCATGATATC+AGG 0.383273 5.4:+24954016 None:intergenic
TCATCACCTTGCACAAGAAA+TGG 0.391236 5.4:-24954849 None:intergenic
CCTAAACCGGTCCACCATAA+AGG 0.397698 5.4:+24954771 MS.gene43906:CDS
AGGGATTAGATTTGAGGTAA+TGG 0.398286 5.4:+24955340 MS.gene43906:CDS
AAATGGAAAGTGAGATTAGA+AGG 0.401729 5.4:+24955267 MS.gene43906:CDS
CCTTCGGTTTATTATGAGAA+TGG 0.405122 5.4:+24954996 MS.gene43906:CDS
TTGGTTTATTAATGTTCCTT+CGG 0.408464 5.4:+24954980 MS.gene43906:CDS
TCCTTCTCACCCTTTCCATA+AGG 0.413581 5.4:-24955236 None:intergenic
GGTTTATTATGAGAATGGTT+TGG 0.433915 5.4:+24955001 MS.gene43906:CDS
TAGTAAAGGTAGTTTGAATC+AGG 0.434714 5.4:-24954597 None:intergenic
TGCAAAATGATTGTGTTCAT+TGG 0.437679 5.4:+24955453 MS.gene43906:CDS
AACTGTTTCAAAACCTAAAC+CGG 0.442193 5.4:+24954758 MS.gene43906:CDS
ATTTGGAGGGATTAGATTTG+AGG 0.443087 5.4:+24955334 MS.gene43906:CDS
AGTGTAATTAAGACCGAAAC+CGG 0.443307 5.4:-24955045 None:intergenic
TTGTGTTATCGTTTGGCAAT+TGG 0.445306 5.4:+24954961 MS.gene43906:CDS
GTTGCTAAAGGGAATGGAAT+TGG 0.445312 5.4:+24955137 MS.gene43906:CDS
CATCCATGTGACTACTTCAA+TGG 0.447132 5.4:+24954483 MS.gene43906:CDS
ACACCGTTAGCGAACGGAAA+AGG 0.447241 5.4:-24955419 None:intergenic
AAATGTATTGTATTAAAACT+TGG 0.452232 5.4:-24954888 None:intergenic
AAGGATTGAGATTGAAGAAG+TGG 0.457929 5.4:+24955286 MS.gene43906:CDS
AATAAAGGTCCTTTATATAG+TGG 0.460409 5.4:+24954522 MS.gene43906:CDS
TAAGCTCCTGGATGACCATC+TGG 0.467024 5.4:-24955389 None:intergenic
GGTAATGGATGTAACTGAAT+TGG 0.467735 5.4:+24955355 MS.gene43906:CDS
ATGTCTCACTCTTCACTCCT+TGG 0.471169 5.4:-24955551 None:intergenic
GATGAAGAAGTGGGATTGGA+AGG 0.471664 5.4:+24955523 MS.gene43906:CDS
TTGAGAGGAAAGTTGCTAAA+GGG 0.472700 5.4:+24955126 MS.gene43906:CDS
TTGAGACCAGATGGTCATCC+AGG 0.475196 5.4:+24955383 MS.gene43906:CDS
ATGCAACATTATCCGTGTAT+TGG 0.475380 5.4:+24954823 MS.gene43906:CDS
GGTGATGAAAGATCAAAATC+CGG 0.476546 5.4:+24954864 MS.gene43906:CDS
ATCTCATTCCAAGTATCTAT+AGG 0.478611 5.4:-24955488 None:intergenic
GTGTTCATTGGTGTTTGCCT+GGG 0.480611 5.4:+24955465 MS.gene43906:CDS
CTTAGTTGGAGAAAAGTGTC+AGG 0.481705 5.4:-24954660 None:intergenic
TGCCTGGGCCTATAGATACT+TGG 0.482132 5.4:+24955480 MS.gene43906:CDS
CATGTTGTTCCACTATATAA+AGG 0.487924 5.4:-24954531 None:intergenic
ACCATAAAGGTTCACATTGG+TGG 0.496277 5.4:+24954784 MS.gene43906:CDS
TCTAGAGATGATGAAGAAGT+GGG 0.498464 5.4:+24955514 MS.gene43906:CDS
AAAGGATTCATATAAGCTCC+TGG 0.500215 5.4:-24955401 None:intergenic
TTGGTTTCCATCTATAGTAA+AGG 0.502522 5.4:-24954611 None:intergenic
TATTGTATTAAAACTTGGCC+CGG 0.503179 5.4:-24954883 None:intergenic
TGTGAACCTTTATGGTGGAC+CGG 0.509927 5.4:-24954777 None:intergenic
TTCTAGAGATGATGAAGAAG+TGG 0.512711 5.4:+24955513 MS.gene43906:CDS
AAGACTGAACCTTATGGAAA+GGG 0.515408 5.4:+24955227 MS.gene43906:CDS
AGGAAAGTTGCTAAAGGGAA+TGG 0.518826 5.4:+24955131 MS.gene43906:CDS
TGGTCGCCATTTCTTGTGCA+AGG 0.526450 5.4:+24954843 MS.gene43906:CDS
ATTGAGAGGAAAGTTGCTAA+AGG 0.529202 5.4:+24955125 MS.gene43906:CDS
AAAGTGTCAGGTTCAAACCT+TGG 0.538655 5.4:-24954648 None:intergenic
CAAATGAGTGCAATCTTCCA+AGG 0.538693 5.4:+24954631 MS.gene43906:CDS
GTGATGAAAGATCAAAATCC+GGG 0.544715 5.4:+24954865 MS.gene43906:CDS
AAAGACTGAACCTTATGGAA+AGG 0.548583 5.4:+24955226 MS.gene43906:CDS
GTTTGAATCAGGTCTTCCAT+TGG 0.554246 5.4:-24954586 None:intergenic
GATGGTGATTGGGATAAGGC+TGG 0.558637 5.4:+24955194 MS.gene43906:CDS
GGACAAAATTGTATCACCAA+TGG 0.563168 5.4:+24954570 MS.gene43906:CDS
TTCCAAGTATCTATAGGCCC+AGG 0.563711 5.4:-24955482 None:intergenic
ACATGTGGAACAATCAAAGA+AGG 0.566099 5.4:+24954549 MS.gene43906:CDS
ATGAAGAAGTGGGATTGGAA+GGG 0.584115 5.4:+24955524 MS.gene43906:CDS
ACCTTATGGAAAGGGTGAGA+AGG 0.593453 5.4:+24955235 MS.gene43906:CDS
TTATATAGTGGAACAACATG+TGG 0.594064 5.4:+24954534 MS.gene43906:CDS
CAATCACCATCAAAATGAGA+AGG 0.607997 5.4:-24955182 None:intergenic
AAGAAATGGCGACCAATACA+CGG 0.614637 5.4:-24954835 None:intergenic
TTTCCATTGAAGTAGTCACA+TGG 0.617272 5.4:-24954486 None:intergenic
TTCGGAACACCGTTAGCGAA+CGG 0.617341 5.4:-24955425 None:intergenic
GTCTTAATAGCATAATTGAG+AGG 0.633582 5.4:+24955111 MS.gene43906:CDS
TTGGCATTGTTGAGACCAGA+TGG 0.642859 5.4:+24955374 MS.gene43906:CDS
GGGATTGGAAGGGACAGCCA+AGG 0.651431 5.4:+24955534 MS.gene43906:CDS
CCATTCTCATAATAAACCGA+AGG 0.655142 5.4:-24954996 None:intergenic
ACCAATGTGAACCTTTATGG+TGG 0.671348 5.4:-24954782 None:intergenic
TCCACCATAAAGGTTCACAT+TGG 0.672090 5.4:+24954781 MS.gene43906:CDS
AAATTGGCGAAGGATATGGA+TGG 0.672881 5.4:+24954927 MS.gene43906:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAATAAATCTAATCAAA+GGG - chr5.4:24954374-24954393 None:intergenic 10.0%
!! AAATGTATTGTATTAAAACT+TGG - chr5.4:24954891-24954910 None:intergenic 15.0%
!! GTTTAAATAAATCTAATCAA+AGG - chr5.4:24954375-24954394 None:intergenic 15.0%
!! TTAAATAAATCTAATCAAAG+GGG - chr5.4:24954373-24954392 None:intergenic 15.0%
!!! AAGTTTTAATACAATACATT+TGG + chr5.4:24954890-24954909 MS.gene43906:CDS 15.0%
!! AATATATCTTTCGATAAATC+TGG - chr5.4:24954087-24954106 None:intergenic 20.0%
!!! TTAAAAGTGTAAAGAGTTTT+CGG - chr5.4:24954280-24954299 None:intergenic 20.0%
!!! TTTTAACTCATTTTGTCTAT+CGG + chr5.4:24954120-24954139 MS.gene43906:intron 20.0%
!!! TTTTTATGTTCCATTAAGAA+AGG + chr5.4:24955076-24955095 MS.gene43906:CDS 20.0%
! AAGAAAAAAGCAAATGCATT+TGG + chr5.4:24955317-24955336 MS.gene43906:CDS 25.0%
! AATAAAGGTCCTTTATATAG+TGG + chr5.4:24954522-24954541 MS.gene43906:CDS 25.0%
! TATCGAAAGATATATTTCGA+TGG + chr5.4:24954091-24954110 MS.gene43906:intron 25.0%
! TTGGTTTATTAATGTTCCTT+CGG + chr5.4:24954980-24954999 MS.gene43906:CDS 25.0%
! TTTAATGAGTGTGTCTTATA+AGG + chr5.4:24954295-24954314 MS.gene43906:intron 25.0%
!! AAATGGATTCATGACAATAA+AGG + chr5.4:24954507-24954526 MS.gene43906:CDS 25.0%
!! AACAAACACATATCTTTTGT+TGG + chr5.4:24954687-24954706 MS.gene43906:CDS 25.0%
!!! TTGTATATCAGAGGTTTTAA+AGG - chr5.4:24954327-24954346 None:intergenic 25.0%
AAAAAAGCAAATGCATTTGG+AGG + chr5.4:24955320-24955339 MS.gene43906:CDS 30.0%
AAAAAGCAAATGCATTTGGA+GGG + chr5.4:24955321-24955340 MS.gene43906:CDS 30.0%
AAACCTCTGATATACAATCA+TGG + chr5.4:24954330-24954349 MS.gene43906:intron 30.0%
AAATGGAAAGTGAGATTAGA+AGG + chr5.4:24955267-24955286 MS.gene43906:CDS 30.0%
AACCATCTACAAAATTGTCA+CGG + chr5.4:24954202-24954221 MS.gene43906:intron 30.0%
AACCTCTGATATACAATCAT+GGG + chr5.4:24954331-24954350 MS.gene43906:intron 30.0%
AACTGTTTCAAAACCTAAAC+CGG + chr5.4:24954758-24954777 MS.gene43906:CDS 30.0%
ATCTCATTCCAAGTATCTAT+AGG - chr5.4:24955491-24955510 None:intergenic 30.0%
CAAACTACCTTTACTATAGA+TGG + chr5.4:24954604-24954623 MS.gene43906:CDS 30.0%
CATGTTGTTCCACTATATAA+AGG - chr5.4:24954534-24954553 None:intergenic 30.0%
GGTCTTAATTACACTGATAT+TGG + chr5.4:24955053-24955072 MS.gene43906:CDS 30.0%
GGTTTATTATGAGAATGGTT+TGG + chr5.4:24955001-24955020 MS.gene43906:CDS 30.0%
GTCTTAATAGCATAATTGAG+AGG + chr5.4:24955111-24955130 MS.gene43906:CDS 30.0%
TACAAAGAAGAGACTCAAAT+TGG - chr5.4:24954731-24954750 None:intergenic 30.0%
TAGTAAAGGTAGTTTGAATC+AGG - chr5.4:24954600-24954619 None:intergenic 30.0%
TATCGGAATATATGTTTCGA+TGG + chr5.4:24954137-24954156 MS.gene43906:intron 30.0%
TATTGTATTAAAACTTGGCC+CGG - chr5.4:24954886-24954905 None:intergenic 30.0%
TGAGAAGGAAATTGGAAAAA+TGG + chr5.4:24955250-24955269 MS.gene43906:CDS 30.0%
TGCAAAATGATTGTGTTCAT+TGG + chr5.4:24955453-24955472 MS.gene43906:CDS 30.0%
TTAGGATGCTTAAATTGTTC+CGG + chr5.4:24955026-24955045 MS.gene43906:CDS 30.0%
TTCCATTAAGAAAGGCTTTA+AGG + chr5.4:24955084-24955103 MS.gene43906:CDS 30.0%
TTGGTTTCCATCTATAGTAA+AGG - chr5.4:24954614-24954633 None:intergenic 30.0%
! CAATCATTTTGCACATACTT+CGG - chr5.4:24955446-24955465 None:intergenic 30.0%
! TTATATAGTGGAACAACATG+TGG + chr5.4:24954534-24954553 MS.gene43906:CDS 30.0%
! TTTTCACCTTCTCATTTTGA+TGG + chr5.4:24955176-24955195 MS.gene43906:CDS 30.0%
!!! TATGAGAATGGTTTGGTTTT+AGG + chr5.4:24955008-24955027 MS.gene43906:CDS 30.0%
!!! TGTGTTTGTTTTTGCTTAGT+TGG - chr5.4:24954677-24954696 None:intergenic 30.0%
!!! TTCTCATTTTGATGGTGATT+GGG + chr5.4:24955184-24955203 MS.gene43906:CDS 30.0%
AAAGACTGAACCTTATGGAA+AGG + chr5.4:24955226-24955245 MS.gene43906:CDS 35.0%
AAAGGATTCATATAAGCTCC+TGG - chr5.4:24955404-24955423 None:intergenic 35.0%
AAGACTGAACCTTATGGAAA+GGG + chr5.4:24955227-24955246 MS.gene43906:CDS 35.0%
AAGGATTGAGATTGAAGAAG+TGG + chr5.4:24955286-24955305 MS.gene43906:CDS 35.0%
ACATGTGGAACAATCAAAGA+AGG + chr5.4:24954549-24954568 MS.gene43906:CDS 35.0%
AGTGTAATTAAGACCGAAAC+CGG - chr5.4:24955048-24955067 None:intergenic 35.0%
ATGCAACATTATCCGTGTAT+TGG + chr5.4:24954823-24954842 MS.gene43906:CDS 35.0%
ATTGAGAGGAAAGTTGCTAA+AGG + chr5.4:24955125-24955144 MS.gene43906:CDS 35.0%
ATTTGGAGGGATTAGATTTG+AGG + chr5.4:24955334-24955353 MS.gene43906:CDS 35.0%
CAATCACCATCAAAATGAGA+AGG - chr5.4:24955185-24955204 None:intergenic 35.0%
CCATTCTCATAATAAACCGA+AGG - chr5.4:24954999-24955018 None:intergenic 35.0%
CCTTCGGTTTATTATGAGAA+TGG + chr5.4:24954996-24955015 MS.gene43906:CDS 35.0%
GACTTGATTGTGTTATCGTT+TGG + chr5.4:24954954-24954973 MS.gene43906:CDS 35.0%
GGACAAAATTGTATCACCAA+TGG + chr5.4:24954570-24954589 MS.gene43906:CDS 35.0%
GGTGATGAAAGATCAAAATC+CGG + chr5.4:24954864-24954883 MS.gene43906:CDS 35.0%
GTCCTTAAAGCCTTTCTTAA+TGG - chr5.4:24955089-24955108 None:intergenic 35.0%
GTGACTACTTCAATGGAAAA+TGG + chr5.4:24954490-24954509 MS.gene43906:CDS 35.0%
GTGATGAAAGATCAAAATCC+GGG + chr5.4:24954865-24954884 MS.gene43906:CDS 35.0%
TCTAGAGATGATGAAGAAGT+GGG + chr5.4:24955514-24955533 MS.gene43906:CDS 35.0%
TGCTTAAATTGTTCCGGTTT+CGG + chr5.4:24955032-24955051 MS.gene43906:CDS 35.0%
TGTTCAAAGACTGAACCTTA+TGG + chr5.4:24955221-24955240 MS.gene43906:CDS 35.0%
TTCCACGTATTGCTTAAAAC+AGG - chr5.4:24954172-24954191 None:intergenic 35.0%
TTGAGAGGAAAGTTGCTAAA+GGG + chr5.4:24955126-24955145 MS.gene43906:CDS 35.0%
TTGTGTTATCGTTTGGCAAT+TGG + chr5.4:24954961-24954980 MS.gene43906:CDS 35.0%
TTTCCATTGAAGTAGTCACA+TGG - chr5.4:24954489-24954508 None:intergenic 35.0%
TTTGTAGATGGTTGCTCTTT+AGG - chr5.4:24954195-24954214 None:intergenic 35.0%
! AACCGTGACAATTTTGTAGA+TGG - chr5.4:24954207-24954226 None:intergenic 35.0%
! AGGGATTAGATTTGAGGTAA+TGG + chr5.4:24955340-24955359 MS.gene43906:CDS 35.0%
! TGTCACGGTTTAGATGTTTT+GGG + chr5.4:24954217-24954236 MS.gene43906:intron 35.0%
! TTCTAGAGATGATGAAGAAG+TGG + chr5.4:24955513-24955532 MS.gene43906:CDS 35.0%
! TTGTCACGGTTTAGATGTTT+TGG + chr5.4:24954216-24954235 MS.gene43906:intron 35.0%
!! GGTAATGGATGTAACTGAAT+TGG + chr5.4:24955355-24955374 MS.gene43906:CDS 35.0%
!! TTCCTGTTTTAAGCAATACG+TGG + chr5.4:24954167-24954186 MS.gene43906:intron 35.0%
!!! CTTCTCATTTTGATGGTGAT+TGG + chr5.4:24955183-24955202 MS.gene43906:CDS 35.0%
!!! TTTTGATGGTGATTGGGATA+AGG + chr5.4:24955190-24955209 MS.gene43906:CDS 35.0%
AAATTGGCGAAGGATATGGA+TGG + chr5.4:24954927-24954946 MS.gene43906:CDS 40.0%
AAGAAATGGCGACCAATACA+CGG - chr5.4:24954838-24954857 None:intergenic 40.0%
ACCAATGTGAACCTTTATGG+TGG - chr5.4:24954785-24954804 None:intergenic 40.0%
ACCATAAAGGTTCACATTGG+TGG + chr5.4:24954784-24954803 MS.gene43906:CDS 40.0%
AGATGATGAAGAAGTGGGAT+TGG + chr5.4:24955519-24955538 MS.gene43906:CDS 40.0%
AGGAAAGTTGCTAAAGGGAA+TGG + chr5.4:24955131-24955150 MS.gene43906:CDS 40.0%
AGTGCTTCATAGCAATATGG+AGG - chr5.4:24954064-24954083 None:intergenic 40.0%
ATGAAGAAGTGGGATTGGAA+GGG + chr5.4:24955524-24955543 MS.gene43906:CDS 40.0%
CAAATGAGTGCAATCTTCCA+AGG + chr5.4:24954631-24954650 MS.gene43906:CDS 40.0%
CACCCATGATTGTATATCAG+AGG - chr5.4:24954336-24954355 None:intergenic 40.0%
CATCCATGTGACTACTTCAA+TGG + chr5.4:24954483-24954502 MS.gene43906:CDS 40.0%
CGAAAAATTGGCGAAGGATA+TGG + chr5.4:24954923-24954942 MS.gene43906:CDS 40.0%
CGTTAACGAAAAATTGGCGA+AGG + chr5.4:24954917-24954936 MS.gene43906:CDS 40.0%
CTTGGAAGATTGCACTCATT+TGG - chr5.4:24954633-24954652 None:intergenic 40.0%
GGATCGCGTTAACGAAAAAT+TGG + chr5.4:24954911-24954930 MS.gene43906:CDS 40.0%
GTGCTTCATAGCAATATGGA+GGG - chr5.4:24954063-24954082 None:intergenic 40.0%
GTTGCTAAAGGGAATGGAAT+TGG + chr5.4:24955137-24955156 MS.gene43906:CDS 40.0%
GTTTGAATCAGGTCTTCCAT+TGG - chr5.4:24954589-24954608 None:intergenic 40.0%
TCATCACCTTGCACAAGAAA+TGG - chr5.4:24954852-24954871 None:intergenic 40.0%
TCCACCATAAAGGTTCACAT+TGG + chr5.4:24954781-24954800 MS.gene43906:CDS 40.0%
! AAAGTGTCAGGTTCAAACCT+TGG - chr5.4:24954651-24954670 None:intergenic 40.0%
! AATCCTTTTCCGTTCGCTAA+CGG + chr5.4:24955416-24955435 MS.gene43906:CDS 40.0%
! TGGAGTGCTTCATAGCAATA+TGG - chr5.4:24954067-24954086 None:intergenic 40.0%
!! CTTAGTTGGAGAAAAGTGTC+AGG - chr5.4:24954663-24954682 None:intergenic 40.0%
ACCTTATGGAAAGGGTGAGA+AGG + chr5.4:24955235-24955254 MS.gene43906:CDS 45.0%
GATGAAGAAGTGGGATTGGA+AGG + chr5.4:24955523-24955542 MS.gene43906:CDS 45.0%
GCCACCAATGTGAACCTTTA+TGG - chr5.4:24954788-24954807 None:intergenic 45.0%
GGAAAGGGTGAGAAGGAAAT+TGG + chr5.4:24955242-24955261 MS.gene43906:CDS 45.0%
TCCTTCTCACCCTTTCCATA+AGG - chr5.4:24955239-24955258 None:intergenic 45.0%
TCGGTGTAACTAAACTCCGA+GGG - chr5.4:24954261-24954280 None:intergenic 45.0%
TTCCAAGTATCTATAGGCCC+AGG - chr5.4:24955485-24955504 None:intergenic 45.0%
TTGGCATTGTTGAGACCAGA+TGG + chr5.4:24955374-24955393 MS.gene43906:CDS 45.0%
! GTGTTCATTGGTGTTTGCCT+GGG + chr5.4:24955465-24955484 MS.gene43906:CDS 45.0%
! TGTGTTCATTGGTGTTTGCC+TGG + chr5.4:24955464-24955483 MS.gene43906:CDS 45.0%
! TTCGGTGTAACTAAACTCCG+AGG - chr5.4:24954262-24954281 None:intergenic 45.0%
!! ACGGTTTAGATGTTTTGGGC+CGG + chr5.4:24954221-24954240 MS.gene43906:intron 45.0%
!! AGATGTTTTGGGCCGGTTAA+TGG + chr5.4:24954228-24954247 MS.gene43906:intron 45.0%
!! TGTGAACCTTTATGGTGGAC+CGG - chr5.4:24954780-24954799 None:intergenic 45.0%
ACACCGTTAGCGAACGGAAA+AGG - chr5.4:24955422-24955441 None:intergenic 50.0%
CCTAAACCGGTCCACCATAA+AGG + chr5.4:24954771-24954790 MS.gene43906:CDS 50.0%
GATGGTGATTGGGATAAGGC+TGG + chr5.4:24955194-24955213 MS.gene43906:CDS 50.0%
TAAGCTCCTGGATGACCATC+TGG - chr5.4:24955392-24955411 None:intergenic 50.0%
TGCCTGGGCCTATAGATACT+TGG + chr5.4:24955480-24955499 MS.gene43906:CDS 50.0%
TGGTCGCCATTTCTTGTGCA+AGG + chr5.4:24954843-24954862 MS.gene43906:CDS 50.0%
TTCGGAACACCGTTAGCGAA+CGG - chr5.4:24955428-24955447 None:intergenic 50.0%
TTGAGACCAGATGGTCATCC+AGG + chr5.4:24955383-24955402 MS.gene43906:CDS 50.0%
!! CCTTTATGGTGGACCGGTTT+AGG - chr5.4:24954774-24954793 None:intergenic 50.0%
AACTAAACTCCGAGGGCCGA+GGG - chr5.4:24954254-24954273 None:intergenic 55.0%
TAACTAAACTCCGAGGGCCG+AGG - chr5.4:24954255-24954274 None:intergenic 55.0%
GGGATTGGAAGGGACAGCCA+AGG + chr5.4:24955534-24955553 MS.gene43906:CDS 60.0%
TTGGGCCGGTTAATGGCCCT+CGG + chr5.4:24954235-24954254 MS.gene43906:intron 60.0%
GAGGGCCGAGGGCCATTAAC+CGG - chr5.4:24954243-24954262 None:intergenic 65.0%
GGTTAATGGCCCTCGGCCCT+CGG + chr5.4:24954242-24954261 MS.gene43906:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 24954028 24955568 24954028 ID=MS.gene43906
chr5.4 mRNA 24954028 24955568 24954028 ID=MS.gene43906.t1;Parent=MS.gene43906
chr5.4 exon 24954028 24954037 24954028 ID=MS.gene43906.t1.exon1;Parent=MS.gene43906.t1
chr5.4 CDS 24954028 24954037 24954028 ID=cds.MS.gene43906.t1;Parent=MS.gene43906.t1
chr5.4 exon 24954457 24955568 24954457 ID=MS.gene43906.t1.exon2;Parent=MS.gene43906.t1
chr5.4 CDS 24954457 24955568 24954457 ID=cds.MS.gene43906.t1;Parent=MS.gene43906.t1
Gene Sequence

>MS.gene43906

ATGATATCAGGTATAGGTAGTTGCTCTTTAAGCCCTCCATATTGCTATGAAGCACTCCAGATTTATCGAAAGATATATTTCGATGGTGTACTTTTTAACTCATTTTGTCTATCGGAATATATGTTTCGATGGTGTATTTTTCCTGTTTTAAGCAATACGTGGAACCTAAAGAGCAACCATCTACAAAATTGTCACGGTTTAGATGTTTTGGGCCGGTTAATGGCCCTCGGCCCTCGGAGTTTAGTTACACCGAAAACTCTTTACACTTTTAATGAGTGTGTCTTATAAGGATTGTACCTTTAAAACCTCTGATATACAATCATGGGTGTATTGTCAACTGAGCCCCTTTGATTAGATTTATTTAAACTATTTAGAATATTAGAATTTATATTTATACATGTAATTAGCACATTGATATTCTATTGACAGTAAATGACAAACACAAAATTGATGAGCATCCATGTGACTACTTCAATGGAAAATGGATTCATGACAATAAAGGTCCTTTATATAGTGGAACAACATGTGGAACAATCAAAGAAGGACAAAATTGTATCACCAATGGAAGACCTGATTCAAACTACCTTTACTATAGATGGAAACCAAATGAGTGCAATCTTCCAAGGTTTGAACCTGACACTTTTCTCCAACTAAGCAAAAACAAACACATATCTTTTGTTGGTGATTCTTTAGCTAGAAACCAATTTGAGTCTCTTCTTTGTATGTTAGCAACTGTTTCAAAACCTAAACCGGTCCACCATAAAGGTTCACATTGGTGGCATTTTTCTTCTCATAATGCAACATTATCCGTGTATTGGTCGCCATTTCTTGTGCAAGGTGATGAAAGATCAAAATCCGGGCCAAGTTTTAATACAATACATTTGGATCGCGTTAACGAAAAATTGGCGAAGGATATGGATGGAATCGACTTGATTGTGTTATCGTTTGGCAATTGGTTTATTAATGTTCCTTCGGTTTATTATGAGAATGGTTTGGTTTTAGGATGCTTAAATTGTTCCGGTTTCGGTCTTAATTACACTGATATTGGTTTTTATGTTCCATTAAGAAAGGCTTTAAGGACTTGTCTTAATAGCATAATTGAGAGGAAAGTTGCTAAAGGGAATGGAATTGGTGTAATTGTGAAAACTTTTTCACCTTCTCATTTTGATGGTGATTGGGATAAGGCTGGTACATGTTCAAAGACTGAACCTTATGGAAAGGGTGAGAAGGAAATTGGAAAAATGGAAAGTGAGATTAGAAGGATTGAGATTGAAGAAGTGGAAAATGCTAAGAAAAAAGCAAATGCATTTGGAGGGATTAGATTTGAGGTAATGGATGTAACTGAATTGGCATTGTTGAGACCAGATGGTCATCCAGGAGCTTATATGAATCCTTTTCCGTTCGCTAACGGTGTTCCGAAGTATGTGCAAAATGATTGTGTTCATTGGTGTTTGCCTGGGCCTATAGATACTTGGAATGAGATTTTTCTAGAGATGATGAAGAAGTGGGATTGGAAGGGACAGCCAAGGAGTGAAGAGTGA
Protein sequence

>MS.gene43906.t1

MISVNDKHKIDEHPCDYFNGKWIHDNKGPLYSGTTCGTIKEGQNCITNGRPDSNYLYYRWKPNECNLPRFEPDTFLQLSKNKHISFVGDSLARNQFESLLCMLATVSKPKPVHHKGSHWWHFSSHNATLSVYWSPFLVQGDERSKSGPSFNTIHLDRVNEKLAKDMDGIDLIVLSFGNWFINVPSVYYENGLVLGCLNCSGFGLNYTDIGFYVPLRKALRTCLNSIIERKVAKGNGIGVIVKTFSPSHFDGDWDKAGTCSKTEPYGKGEKEIGKMESEIRRIEIEEVENAKKKANAFGGIRFEVMDVTELALLRPDGHPGAYMNPFPFANGVPKYVQNDCVHWCLPGPIDTWNEIFLEMMKKWDWKGQPRSEE