Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44364.t1 | XP_003613218.1 | 100 | 808 | 0 | 0 | 1 | 808 | 1 | 808 | 0.00E+00 | 1636.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44364.t1 | P42347 | 92.9 | 814 | 52 | 1 | 1 | 808 | 1 | 814 | 0.0e+00 | 1529.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44364.t1 | Q8GUA6 | 100.0 | 808 | 0 | 0 | 1 | 808 | 1 | 808 | 0.0e+00 | 1636.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene44364 | 0.831072 | 2.05E-55 | -1.69E-46 |
| MS.gene049404 | MS.gene44364 | 0.83806 | 3.60E-57 | -1.69E-46 |
| MS.gene049983 | MS.gene44364 | 0.813729 | 2.21E-51 | -1.69E-46 |
| MS.gene050559 | MS.gene44364 | 0.827038 | 1.95E-54 | -1.69E-46 |
| MS.gene050561 | MS.gene44364 | 0.824651 | 7.19E-54 | -1.69E-46 |
| MS.gene050562 | MS.gene44364 | 0.817521 | 3.16E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene44364 | 0.803066 | 4.16E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene44364 | 0.815813 | 7.63E-52 | -1.69E-46 |
| MS.gene051744 | MS.gene44364 | 0.800295 | 1.54E-48 | -1.69E-46 |
| MS.gene052037 | MS.gene44364 | 0.800705 | 1.27E-48 | -1.69E-46 |
| MS.gene052239 | MS.gene44364 | -0.81326 | 2.80E-51 | -1.69E-46 |
| MS.gene052500 | MS.gene44364 | 0.8093 | 2.03E-50 | -1.69E-46 |
| MS.gene052502 | MS.gene44364 | 0.807317 | 5.36E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene44364 | 0.806548 | 7.80E-50 | -1.69E-46 |
| MS.gene052534 | MS.gene44364 | 0.803795 | 2.94E-49 | -1.69E-46 |
| MS.gene052674 | MS.gene44364 | 0.820917 | 5.32E-53 | -1.69E-46 |
| MS.gene052675 | MS.gene44364 | 0.827158 | 1.83E-54 | -1.69E-46 |
| MS.gene052677 | MS.gene44364 | 0.829612 | 4.67E-55 | -1.69E-46 |
| MS.gene052826 | MS.gene44364 | 0.809295 | 2.03E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene44364 | 0.803947 | 2.73E-49 | -1.69E-46 |
| MS.gene053201 | MS.gene44364 | 0.805688 | 1.18E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene44364 | 0.817439 | 3.30E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene44364 | 0.829969 | 3.82E-55 | -1.69E-46 |
| MS.gene055228 | MS.gene44364 | 0.813412 | 2.59E-51 | -1.69E-46 |
| MS.gene055464 | MS.gene44364 | 0.824058 | 9.92E-54 | -1.69E-46 |
| MS.gene055724 | MS.gene44364 | 0.837511 | 4.98E-57 | -1.69E-46 |
| MS.gene056174 | MS.gene44364 | 0.809177 | 2.15E-50 | -1.69E-46 |
| MS.gene056252 | MS.gene44364 | 0.815253 | 1.02E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene44364 | 0.801141 | 1.04E-48 | -1.69E-46 |
| MS.gene056507 | MS.gene44364 | 0.804424 | 2.17E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene44364 | 0.847514 | 1.10E-59 | -1.69E-46 |
| MS.gene057208 | MS.gene44364 | 0.82352 | 1.33E-53 | -1.69E-46 |
| MS.gene057855 | MS.gene44364 | 0.820613 | 6.25E-53 | -1.69E-46 |
| MS.gene057869 | MS.gene44364 | 0.821139 | 4.73E-53 | -1.69E-46 |
| MS.gene058008 | MS.gene44364 | 0.81624 | 6.12E-52 | -1.69E-46 |
| MS.gene058009 | MS.gene44364 | 0.835724 | 1.42E-56 | -1.69E-46 |
| MS.gene058010 | MS.gene44364 | 0.869859 | 2.26E-66 | -1.69E-46 |
| MS.gene058979 | MS.gene44364 | 0.849243 | 3.66E-60 | -1.69E-46 |
| MS.gene059439 | MS.gene44364 | 0.802447 | 5.58E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene44364 | 0.815435 | 9.26E-52 | -1.69E-46 |
| MS.gene060167 | MS.gene44364 | 0.808543 | 2.94E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene44364 | 0.82504 | 5.82E-54 | -1.69E-46 |
| MS.gene060933 | MS.gene44364 | 0.814539 | 1.46E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene44364 | 0.823607 | 1.26E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene44364 | 0.812531 | 4.05E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene66413 | MS.gene44364 | PPI |
| MS.gene66405 | MS.gene44364 | PPI |
| MS.gene059541 | MS.gene44364 | PPI |
| MS.gene063319 | MS.gene44364 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44364.t1 | MTR_5g034120 | 100.000 | 808 | 0 | 0 | 1 | 808 | 1 | 808 | 0.0 | 1670 |
| MS.gene44364.t1 | MTR_3g116250 | 28.778 | 483 | 293 | 19 | 334 | 788 | 1555 | 2014 | 2.81e-34 | 142 |
| MS.gene44364.t1 | MTR_8g093570 | 33.906 | 233 | 141 | 6 | 555 | 777 | 829 | 1058 | 2.90e-27 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44364.t1 | AT1G60490 | 84.049 | 815 | 122 | 4 | 1 | 808 | 1 | 814 | 0.0 | 1415 |
| MS.gene44364.t1 | AT1G49340 | 27.477 | 535 | 336 | 19 | 274 | 781 | 1491 | 2000 | 3.01e-38 | 155 |
| MS.gene44364.t1 | AT1G49340 | 27.477 | 535 | 336 | 19 | 274 | 781 | 1491 | 2000 | 3.01e-38 | 155 |
| MS.gene44364.t1 | AT1G49340 | 27.477 | 535 | 336 | 19 | 274 | 781 | 1484 | 1993 | 3.04e-38 | 155 |
| MS.gene44364.t1 | AT1G49340 | 27.477 | 535 | 336 | 19 | 274 | 781 | 1484 | 1993 | 3.04e-38 | 155 |
| MS.gene44364.t1 | AT5G64070 | 33.617 | 235 | 139 | 7 | 555 | 777 | 861 | 1090 | 5.33e-28 | 122 |
| MS.gene44364.t1 | AT5G09350 | 33.906 | 233 | 141 | 6 | 555 | 777 | 856 | 1085 | 8.18e-28 | 121 |
| MS.gene44364.t1 | AT1G51040 | 28.956 | 297 | 178 | 11 | 511 | 788 | 222 | 504 | 1.95e-19 | 93.2 |
Find 182 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAAAATAGATTTAAAATT+AGG | 0.176506 | 5.1:-25004682 | None:intergenic |
| CCTGATGATCATGGGCCTTT+TGG | 0.206214 | 5.1:+25003129 | MS.gene44364:CDS |
| TAGATTTAAAATTAGGTTAC+TGG | 0.212396 | 5.1:-25004675 | None:intergenic |
| TGTTCAAATGGTATCGTTAA+TGG | 0.238019 | 5.1:+25002181 | MS.gene44364:CDS |
| AAGCTTAGACAGCTACTTTC+TGG | 0.244205 | 5.1:+25001589 | MS.gene44364:CDS |
| GCCAGTCCACTCGTTGAATT+TGG | 0.248295 | 5.1:-24997436 | None:intergenic |
| ATAGCTTCTGATCCTGAAAA+AGG | 0.252329 | 5.1:+25004731 | MS.gene44364:CDS |
| AAGGAAATAGACCTGAATTA+AGG | 0.256047 | 5.1:-24994697 | None:intergenic |
| TTCATCGTTGGGCTCAATAT+TGG | 0.265072 | 5.1:+25004950 | MS.gene44364:CDS |
| CACATTGATGGCGCGCCGTT+TGG | 0.265882 | 5.1:+24995102 | MS.gene44364:CDS |
| ACTTGAACCTTGTATAATAT+TGG | 0.282964 | 5.1:-25004616 | None:intergenic |
| TTTCCCAATTCCCTTAATTC+AGG | 0.290246 | 5.1:+24994686 | MS.gene44364:CDS |
| TTACTGCACATATGAAATTC+TGG | 0.297436 | 5.1:+25000957 | MS.gene44364:CDS |
| TAGACCTGAATTAAGGGAAT+TGG | 0.308027 | 5.1:-24994690 | None:intergenic |
| AGAAGCTATGTCCGGAATAT+TGG | 0.311004 | 5.1:-25004717 | None:intergenic |
| ATGACCCTACATATGCAAAA+AGG | 0.322562 | 5.1:+25000932 | MS.gene44364:CDS |
| TGCACATTATTAACCTTTCC+AGG | 0.328164 | 5.1:-24997248 | None:intergenic |
| TCACGTTAACAGCTAAATAT+CGG | 0.339699 | 5.1:+24996822 | MS.gene44364:CDS |
| AGCTTCATTGGTGGCGGAAA+TGG | 0.339940 | 5.1:-25004450 | None:intergenic |
| AATTTCTTCGTTGTGTTGAA+TGG | 0.348338 | 5.1:+24999425 | MS.gene44364:CDS |
| ATTTCCTTGCAGAGCTTCAT+TGG | 0.349240 | 5.1:-25004462 | None:intergenic |
| TTACTTGCTCCAATTGGTTC+AGG | 0.349986 | 5.1:+25000705 | MS.gene44364:CDS |
| AGCATTAAGCAATATTGAAT+TGG | 0.350124 | 5.1:+25000873 | MS.gene44364:intron |
| GACCAATACCTCATCAAAAT+AGG | 0.353005 | 5.1:-25001629 | None:intergenic |
| AAAGACTTCCATCGTCTCTT+AGG | 0.355332 | 5.1:-25004386 | None:intergenic |
| CCATACAAGGTTCTAGCAAC+TGG | 0.356646 | 5.1:+25002245 | MS.gene44364:CDS |
| CCAATGATATTGTTATTGTC+TGG | 0.358804 | 5.1:+24998481 | MS.gene44364:CDS |
| ATTTAAACGGTGGCAGAGTT+TGG | 0.367837 | 5.1:+25001480 | MS.gene44364:CDS |
| GGAAGCAAAAGCTTAGGCTT+TGG | 0.380644 | 5.1:+24997186 | MS.gene44364:CDS |
| TCGCCTCACATTCAAAACTA+TGG | 0.381640 | 5.1:+24997463 | MS.gene44364:CDS |
| GGAAAAGTTTCGGTTGGACT+TGG | 0.385873 | 5.1:+25004846 | MS.gene44364:CDS |
| ATCACATATATACTTGGTAT+TGG | 0.386539 | 5.1:+25003207 | MS.gene44364:CDS |
| TCTCATGGTGAAGGATTGAT+TGG | 0.388746 | 5.1:+24997116 | MS.gene44364:CDS |
| GAAGCAAAAGCTTAGGCTTT+GGG | 0.388788 | 5.1:+24997187 | MS.gene44364:CDS |
| TTAGGTCCCTAGGCATGAAC+GGG | 0.395425 | 5.1:+24997367 | MS.gene44364:intron |
| TCCAATTGGTTCAGGCTCTT+CGG | 0.397784 | 5.1:+25000713 | MS.gene44364:CDS |
| ATCCCAGTGATGAGCACATC+TGG | 0.403063 | 5.1:-25001834 | None:intergenic |
| TACTTTCTCTCATTTGAGCT+TGG | 0.405483 | 5.1:-24998597 | None:intergenic |
| CCCTACCTGTGCTAATGAAC+GGG | 0.416972 | 5.1:-25002300 | None:intergenic |
| AGGAAATAGACCTGAATTAA+GGG | 0.417957 | 5.1:-24994696 | None:intergenic |
| TTCTTTGATGTCTGAGAAGA+GGG | 0.418191 | 5.1:+24999393 | MS.gene44364:CDS |
| AATGGCGGGTTCCAATATTC+CGG | 0.423699 | 5.1:+25004706 | MS.gene44364:CDS |
| AGACCTGAATTAAGGGAATT+GGG | 0.427233 | 5.1:-24994689 | None:intergenic |
| TACAGCTCCAGGAAAAGTTT+CGG | 0.427922 | 5.1:+25004836 | MS.gene44364:intron |
| TTCCGCATCGAACGCCTTGA+AGG | 0.429980 | 5.1:+24994653 | MS.gene44364:CDS |
| CCAGTTGCTAGAACCTTGTA+TGG | 0.430909 | 5.1:-25002245 | None:intergenic |
| ACACCAGATGTGCTCATCAC+TGG | 0.432555 | 5.1:+25001831 | MS.gene44364:CDS |
| ATTGGTGGCGGAAATGGCTT+AGG | 0.435687 | 5.1:-25004444 | None:intergenic |
| GTTGTATGCTTCACAACAAT+AGG | 0.437263 | 5.1:-25004639 | None:intergenic |
| GAAACAAGCACTAGAATTGA+TGG | 0.437691 | 5.1:+24999966 | MS.gene44364:CDS |
| GAAAGGGTAAATTGCCTTCA+AGG | 0.440046 | 5.1:-24994667 | None:intergenic |
| ATATCATTGGTCGAAGCTAT+AGG | 0.441914 | 5.1:-24998468 | None:intergenic |
| CAAGGAAATGGTTGAGGCTA+TGG | 0.443471 | 5.1:+25004476 | MS.gene44364:CDS |
| AGCCTGTATACATTTCTTCC+AGG | 0.443637 | 5.1:+25004876 | MS.gene44364:CDS |
| AATTGGCAAGCTTTCTTCGC+TGG | 0.446366 | 5.1:+25000890 | MS.gene44364:CDS |
| AATGAAGCTCTGCAAGGAAA+TGG | 0.448445 | 5.1:+25004464 | MS.gene44364:CDS |
| CCCTAACAATTTCACATGAC+TGG | 0.450078 | 5.1:+24994581 | None:intergenic |
| GAGTGTGCGTTGCACATTGA+TGG | 0.450351 | 5.1:+24995090 | MS.gene44364:CDS |
| TCAATGTTACTTGCTCCAAT+TGG | 0.451060 | 5.1:+25000699 | MS.gene44364:CDS |
| CCAAAAGGCCCATGATCATC+AGG | 0.453310 | 5.1:-25003129 | None:intergenic |
| CGCTCTCGTTGATAAGATCC+TGG | 0.454719 | 5.1:-25004894 | None:intergenic |
| GTGAAGCATACAACATTCTT+CGG | 0.454783 | 5.1:+25004648 | MS.gene44364:CDS |
| GGTTGAGACCATTCATCGTT+GGG | 0.455522 | 5.1:+25004939 | MS.gene44364:CDS |
| CCACGGGACCCTCGTATTGT+TGG | 0.464547 | 5.1:+24996789 | MS.gene44364:CDS |
| CTTGTTGTAGAAAATGATGA+TGG | 0.464644 | 5.1:+24995048 | MS.gene44364:intron |
| CCAGACAATAACAATATCAT+TGG | 0.466559 | 5.1:-24998481 | None:intergenic |
| ACCGAAGAGCCTGAACCAAT+TGG | 0.466842 | 5.1:-25000714 | None:intergenic |
| CAATGATATTGTTATTGTCT+GGG | 0.467655 | 5.1:+24998482 | MS.gene44364:CDS |
| GCAGGAATACCAACTGGTCC+TGG | 0.471798 | 5.1:-25001971 | None:intergenic |
| TATAAACGTTTCAAGACAGT+TGG | 0.477924 | 5.1:-25003160 | None:intergenic |
| GGACTTTGAGTGGAGATGAT+AGG | 0.478943 | 5.1:+24999347 | MS.gene44364:CDS |
| CTGGTGAAGGTTGGAGTCCA+CGG | 0.480995 | 5.1:+24996772 | MS.gene44364:intron |
| TGGGATCTTTCTCATGGTGA+AGG | 0.482025 | 5.1:+24997107 | MS.gene44364:CDS |
| TCTCCACTCAAAGTCCTTGT+TGG | 0.482283 | 5.1:-24999340 | None:intergenic |
| GCCAAATTCAACGAGTGGAC+TGG | 0.484095 | 5.1:+24997435 | MS.gene44364:CDS |
| CTTGCAGCCAATATTATACA+AGG | 0.485228 | 5.1:+25004609 | MS.gene44364:intron |
| CACGTTAACAGCTAAATATC+GGG | 0.491737 | 5.1:+24996823 | MS.gene44364:CDS |
| TCAGGATCAGAAGCTATGTC+CGG | 0.491939 | 5.1:-25004725 | None:intergenic |
| CAGGTTTGGGATCTTTCTCA+TGG | 0.498015 | 5.1:+24997101 | MS.gene44364:intron |
| CACCAGATGTGCTCATCACT+GGG | 0.498800 | 5.1:+25001832 | MS.gene44364:CDS |
| ATCCTGGAAGAAATGTATAC+AGG | 0.501151 | 5.1:-25004878 | None:intergenic |
| ACCAAGTATATATGTGATAA+CGG | 0.501900 | 5.1:-25003202 | None:intergenic |
| GACACCACGGGTTAAACTCC+TGG | 0.502225 | 5.1:-24998556 | None:intergenic |
| TGTGTCCTCATTAGGTCCCT+AGG | 0.503314 | 5.1:+24997357 | MS.gene44364:intron |
| ACCTTTCCAGGTGTAGAAGT+AGG | 0.504443 | 5.1:-24997236 | None:intergenic |
| ATATTGAGCCCAACGATGAA+TGG | 0.507424 | 5.1:-25004947 | None:intergenic |
| TTTAACTGTTTATGCTCAGA+GGG | 0.508499 | 5.1:-24998528 | None:intergenic |
| CCTTTCCAGGTGTAGAAGTA+GGG | 0.509565 | 5.1:-24997235 | None:intergenic |
| ATACTTCAATATTCTCTGAA+TGG | 0.509593 | 5.1:-24999314 | None:intergenic |
| AAGATGATAAAACCTTGTTC+TGG | 0.511758 | 5.1:-24995133 | None:intergenic |
| TCCATCCCGTTCATTAGCAC+AGG | 0.513341 | 5.1:+25002295 | MS.gene44364:CDS |
| ATAAACAGTTAAAGCTGGCC+AGG | 0.513701 | 5.1:+24998538 | MS.gene44364:CDS |
| CGTTCGATGCGGAAAGTGAC+GGG | 0.517013 | 5.1:-24994644 | None:intergenic |
| AAGCTTTCTTCGCTGGTATG+TGG | 0.517061 | 5.1:+25000897 | MS.gene44364:CDS |
| GGTGCAAACGGAGAGGAAGA+TGG | 0.517256 | 5.1:+25001457 | MS.gene44364:CDS |
| CTAGCAACTGGGCAAGATGA+AGG | 0.518120 | 5.1:+25002257 | MS.gene44364:CDS |
| CAAAACTATGGAGAAAATCA+AGG | 0.518753 | 5.1:+24997475 | MS.gene44364:CDS |
| TGGTGAAGGTTGGAGTCCAC+GGG | 0.519827 | 5.1:+24996773 | MS.gene44364:intron |
| CCACTCAAAGTCCTTGTTGG+TGG | 0.523782 | 5.1:-24999337 | None:intergenic |
| CAAAAGCTTAGGCTTTGGGC+AGG | 0.524104 | 5.1:+24997191 | MS.gene44364:CDS |
| TTAAATATTGATGACTCTGA+GGG | 0.524865 | 5.1:-25001861 | None:intergenic |
| CAGATGGGAGCTGGTGCAAA+CGG | 0.525919 | 5.1:+25001445 | MS.gene44364:intron |
| TCCGTTATCACATATATACT+TGG | 0.530140 | 5.1:+25003201 | MS.gene44364:CDS |
| TCCTTGCAGAGCTTCATTGG+TGG | 0.531959 | 5.1:-25004459 | None:intergenic |
| TGTTGAATGGAGTGATGTAC+AGG | 0.532745 | 5.1:+24999438 | MS.gene44364:CDS |
| CCCTACTTCTACACCTGGAA+AGG | 0.534797 | 5.1:+24997235 | MS.gene44364:CDS |
| CAGTTGGCTGTAATTCCAAA+AGG | 0.534886 | 5.1:-25003144 | None:intergenic |
| TTCCATCGTCTCTTAGGAGG+AGG | 0.535524 | 5.1:-25004380 | None:intergenic |
| TTTCTTTGATGTCTGAGAAG+AGG | 0.535885 | 5.1:+24999392 | MS.gene44364:CDS |
| ATGATAAAACCTTGTTCTGG+TGG | 0.536198 | 5.1:-24995130 | None:intergenic |
| TTGCAGAGCTTCATTGGTGG+CGG | 0.538044 | 5.1:-25004456 | None:intergenic |
| GAGTGCATTGTTCCCTCAGA+TGG | 0.538202 | 5.1:+25004918 | MS.gene44364:CDS |
| GATTGACGTTTGTGATGCAC+TGG | 0.539342 | 5.1:+25000002 | MS.gene44364:CDS |
| CCAAAATCTCAGATGGGAGC+TGG | 0.541918 | 5.1:+25001436 | MS.gene44364:intron |
| CACTAGAATTGATGGGCAAG+TGG | 0.544415 | 5.1:+24999974 | MS.gene44364:CDS |
| GCTCCAGGAAAAGTTTCGGT+TGG | 0.551085 | 5.1:+25004840 | MS.gene44364:intron |
| GTTCAATCACTAGAGATGTC+AGG | 0.551302 | 5.1:+25001533 | MS.gene44364:CDS |
| ATTAGGTCCCTAGGCATGAA+CGG | 0.552317 | 5.1:+24997366 | MS.gene44364:intron |
| TCTGCATTTAACTCCATACA+AGG | 0.553089 | 5.1:+25002232 | MS.gene44364:CDS |
| GGTGTCAGAGGTGATCGTAT+TGG | 0.554165 | 5.1:-25001813 | None:intergenic |
| TGAATGGTCTCAACCATCTG+AGG | 0.555119 | 5.1:-25004931 | None:intergenic |
| GTTCTGGTGGGAAGTCCAAA+CGG | 0.556275 | 5.1:-24995117 | None:intergenic |
| AAGGAAATGGTTGAGGCTAT+GGG | 0.556476 | 5.1:+25004477 | MS.gene44364:CDS |
| GCGTTCGATGCGGAAAGTGA+CGG | 0.556749 | 5.1:-24994645 | None:intergenic |
| CATGGTGAAGGATTGATTGG+AGG | 0.557450 | 5.1:+24997119 | MS.gene44364:CDS |
| TGAATTAAGGGAATTGGGAA+AGG | 0.557858 | 5.1:-24994684 | None:intergenic |
| TTTAAATATTGATGACTCTG+AGG | 0.558343 | 5.1:-25001862 | None:intergenic |
| GAGAGGAAGATGGATTTAAA+CGG | 0.558804 | 5.1:+25001467 | MS.gene44364:CDS |
| AGGCTATGGGTGGAGCTGAA+AGG | 0.561462 | 5.1:+25004490 | MS.gene44364:CDS |
| ATATACTTGGTATTGGAGAC+AGG | 0.562402 | 5.1:+25003214 | MS.gene44364:CDS |
| AGACTGGGAAGCAAAAGCTT+AGG | 0.563123 | 5.1:+24997180 | MS.gene44364:CDS |
| ACCCTACCTGTGCTAATGAA+CGG | 0.563767 | 5.1:-25002301 | None:intergenic |
| TGAGCATAAACAGTTAAAGC+TGG | 0.564717 | 5.1:+24998533 | MS.gene44364:CDS |
| TGGTTGAGACCATTCATCGT+TGG | 0.569134 | 5.1:+25004938 | MS.gene44364:CDS |
| TCTGGAGGAGTATATGATGA+AGG | 0.572902 | 5.1:+25000975 | MS.gene44364:CDS |
| TGGGCAAGATGAAGGCATGT+TGG | 0.574569 | 5.1:+25002265 | MS.gene44364:CDS |
| GCTCTGCAAGGAAATGGTTG+AGG | 0.577513 | 5.1:+25004470 | MS.gene44364:CDS |
| GACTTCCATCGTCTCTTAGG+AGG | 0.577553 | 5.1:-25004383 | None:intergenic |
| GAATTAAGGGAATTGGGAAA+GGG | 0.579406 | 5.1:-24994683 | None:intergenic |
| GTTGTTAAAGAGAAGAATTG+TGG | 0.583980 | 5.1:-24997144 | None:intergenic |
| TGCCTTCAAGGCGTTCGATG+CGG | 0.584785 | 5.1:-24994655 | None:intergenic |
| GGTCCCTAGGCATGAACGGG+GGG | 0.585456 | 5.1:+24997370 | MS.gene44364:intron |
| TATTGTCTGGGACCCAGAAG+TGG | 0.588125 | 5.1:+24998494 | MS.gene44364:CDS |
| TCTTCTTCTGTGTATTACCT+CGG | 0.588947 | 5.1:-25001561 | None:intergenic |
| CTTTAACTGTTTATGCTCAG+AGG | 0.591757 | 5.1:-24998529 | None:intergenic |
| TGATAAAACCTTGTTCTGGT+GGG | 0.593117 | 5.1:-24995129 | None:intergenic |
| ATCTCTGTCTATGACACCAC+GGG | 0.595270 | 5.1:-24998568 | None:intergenic |
| TGGAAGTGCTCATTTGTATG+TGG | 0.595283 | 5.1:+24997517 | MS.gene44364:CDS |
| ACTAGAATTGATGGGCAAGT+GGG | 0.598933 | 5.1:+24999975 | MS.gene44364:CDS |
| TTGGACTTCCCACCAGAACA+AGG | 0.599322 | 5.1:+24995121 | MS.gene44364:CDS |
| TTGGTCGAAGCTATAGGAGA+TGG | 0.600468 | 5.1:-24998462 | None:intergenic |
| CCACCAACAAGGACTTTGAG+TGG | 0.604075 | 5.1:+24999337 | MS.gene44364:CDS |
| GAGATGATAGGCAACTCTTG+TGG | 0.604236 | 5.1:+24999359 | MS.gene44364:CDS |
| CGATCTTCGCCAGGACCAGT+TGG | 0.607072 | 5.1:+25001962 | MS.gene44364:CDS |
| ACATTCCCTACTTCTACACC+TGG | 0.610568 | 5.1:+24997230 | MS.gene44364:CDS |
| CATACAAGGTTCTAGCAACT+GGG | 0.611041 | 5.1:+25002246 | MS.gene44364:CDS |
| AAACAAGCACTAGAATTGAT+GGG | 0.614052 | 5.1:+24999967 | MS.gene44364:CDS |
| TGGGCAGGAAAAGAAGCAGA+TGG | 0.617410 | 5.1:+24997206 | MS.gene44364:CDS |
| ACCTGTGCTAATGAACGGGA+TGG | 0.618260 | 5.1:-25002296 | None:intergenic |
| AACCTCCTCCTAAGAGACGA+TGG | 0.624022 | 5.1:+25004378 | MS.gene44364:CDS |
| ATTGTCTGGGACCCAGAAGT+GGG | 0.631740 | 5.1:+24998495 | MS.gene44364:CDS |
| GCCACCAATGAAGCTCTGCA+AGG | 0.636272 | 5.1:+25004458 | MS.gene44364:CDS |
| TGGAACTGCAGAGTTATATG+TGG | 0.636425 | 5.1:+24995068 | MS.gene44364:CDS |
| AGGTCCCTAGGCATGAACGG+GGG | 0.637589 | 5.1:+24997369 | MS.gene44364:intron |
| GAATAAAACCAAAATCAACA+TGG | 0.639157 | 5.1:-25004410 | None:intergenic |
| GAGCTCATTCCAACAATACG+AGG | 0.645146 | 5.1:-24996798 | None:intergenic |
| GCGAGGCCAAATTCAACGAG+TGG | 0.647883 | 5.1:+24997430 | MS.gene44364:CDS |
| GAACACTCACTGCATAAGCA+CGG | 0.655020 | 5.1:-25000651 | None:intergenic |
| GAAATGGTTGAGGCTATGGG+TGG | 0.659442 | 5.1:+25004480 | MS.gene44364:CDS |
| TAGGTCCCTAGGCATGAACG+GGG | 0.661850 | 5.1:+24997368 | MS.gene44364:intron |
| ATGAGCACATCTGGTGTCAG+AGG | 0.665902 | 5.1:-25001825 | None:intergenic |
| GAATGGTCTCAACCATCTGA+GGG | 0.666217 | 5.1:-25004930 | None:intergenic |
| CTGCACATATGAAATTCTGG+AGG | 0.666533 | 5.1:+25000960 | MS.gene44364:CDS |
| ACCCCATGCACATACCACGC+TGG | 0.667482 | 5.1:-25000777 | None:intergenic |
| GGGAGCTGGTGCAAACGGAG+AGG | 0.679178 | 5.1:+25001450 | MS.gene44364:CDS |
| AGGAAGATGGATTTAAACGG+TGG | 0.680685 | 5.1:+25001470 | MS.gene44364:CDS |
| GATCTCTGTCTATGACACCA+CGG | 0.681065 | 5.1:-24998569 | None:intergenic |
| CCAACAATACGAGGGTCCCG+TGG | 0.688862 | 5.1:-24996789 | None:intergenic |
| TATTGAAGTATCCACCAACA+AGG | 0.699350 | 5.1:+24999326 | MS.gene44364:CDS |
| AGAGATGTCAGGAATGTCCG+AGG | 0.705449 | 5.1:+25001544 | MS.gene44364:CDS |
| AGCTCATTCCAACAATACGA+GGG | 0.708520 | 5.1:-24996797 | None:intergenic |
| CTGGCCAGGAGTTTAACCCG+TGG | 0.725449 | 5.1:+24998552 | MS.gene44364:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 24994596 | 25004978 | 24994596 | ID=MS.gene44364 |
| chr5.1 | mRNA | 24994596 | 25004978 | 24994596 | ID=MS.gene44364.t1;Parent=MS.gene44364 |
| chr5.1 | exon | 24994596 | 24994707 | 24994596 | ID=MS.gene44364.t1.exon1;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 24994596 | 24994707 | 24994596 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 24995058 | 24995142 | 24995058 | ID=MS.gene44364.t1.exon2;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 24995058 | 24995142 | 24995058 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 24996781 | 24996877 | 24996781 | ID=MS.gene44364.t1.exon3;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 24996781 | 24996877 | 24996781 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 24997104 | 24997256 | 24997104 | ID=MS.gene44364.t1.exon4;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 24997104 | 24997256 | 24997104 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 24997371 | 24997580 | 24997371 | ID=MS.gene44364.t1.exon5;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 24997371 | 24997580 | 24997371 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 24998438 | 24998616 | 24998438 | ID=MS.gene44364.t1.exon6;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 24998438 | 24998616 | 24998438 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 24999312 | 24999459 | 24999312 | ID=MS.gene44364.t1.exon7;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 24999312 | 24999459 | 24999312 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 24999961 | 25000056 | 24999961 | ID=MS.gene44364.t1.exon8;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 24999961 | 25000056 | 24999961 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 25000649 | 25000784 | 25000649 | ID=MS.gene44364.t1.exon9;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 25000649 | 25000784 | 25000649 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 25000875 | 25000996 | 25000875 | ID=MS.gene44364.t1.exon10;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 25000875 | 25000996 | 25000875 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 25001448 | 25001642 | 25001448 | ID=MS.gene44364.t1.exon11;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 25001448 | 25001642 | 25001448 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 25001813 | 25001983 | 25001813 | ID=MS.gene44364.t1.exon12;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 25001813 | 25001983 | 25001813 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 25002179 | 25002316 | 25002179 | ID=MS.gene44364.t1.exon13;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 25002179 | 25002316 | 25002179 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 25003081 | 25003235 | 25003081 | ID=MS.gene44364.t1.exon14;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 25003081 | 25003235 | 25003081 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 25004368 | 25004511 | 25004368 | ID=MS.gene44364.t1.exon15;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 25004368 | 25004511 | 25004368 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 25004616 | 25004763 | 25004616 | ID=MS.gene44364.t1.exon16;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 25004616 | 25004763 | 25004616 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| chr5.1 | exon | 25004841 | 25004978 | 25004841 | ID=MS.gene44364.t1.exon17;Parent=MS.gene44364.t1 |
| chr5.1 | CDS | 25004841 | 25004978 | 25004841 | ID=cds.MS.gene44364.t1;Parent=MS.gene44364.t1 |
| Gene Sequence |
| Protein sequence |
