AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45686

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45686.t1 MTR_4g069920 99.096 885 8 0 1 885 1 885 0.0 1793
MS.gene45686.t1 MTR_3g116480 89.640 888 88 2 1 885 1 887 0.0 1641
MS.gene45686.t1 MTR_4g076210 20.189 634 439 17 21 611 9 618 5.37e-16 83.2
MS.gene45686.t1 MTR_4g008250 22.816 412 267 13 48 433 36 422 1.50e-11 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45686.t1 AT4G34450 85.344 887 127 2 1 885 1 886 0.0 1565
MS.gene45686.t1 AT2G16200 63.768 69 25 0 817 885 1 69 1.88e-24 97.8
MS.gene45686.t1 AT2G16200 63.077 65 24 0 821 885 19 83 4.72e-22 91.7
MS.gene45686.t1 AT4G23460 19.713 558 393 16 21 549 9 540 1.09e-13 75.9
MS.gene45686.t1 AT4G11380 19.604 556 396 15 21 549 9 540 1.92e-12 72.0
MS.gene45686.t1 AT4G11380 19.739 537 380 15 40 549 50 562 3.33e-12 71.2

Find 216 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCAAATACCGCTCAATT+TGG 0.166022 4.4:+37937640 None:intergenic
ATTTCCTGAGTAACATTCTT+AGG 0.185985 4.4:-37935402 MS.gene45686:CDS
GGTTTGGGTGCTCCTCCTAT+TGG 0.249762 4.4:-37934404 MS.gene45686:CDS
GTACTTGTGCGGCTGTCTTT+TGG 0.253038 4.4:-37932270 MS.gene45686:CDS
TGCTTCTGTTGATGGATTAA+AGG 0.267375 4.4:-37935016 MS.gene45686:intron
ATCAACAGAAGCACCAAATT+TGG 0.272603 4.4:+37935026 None:intergenic
GTTTGGGTGCTCCTCCTATT+GGG 0.277259 4.4:-37934403 MS.gene45686:CDS
TACATCTTCCAATAGTTGTT+TGG 0.292920 4.4:+37934086 None:intergenic
GGGAGAGTTCCTTTATTATC+AGG 0.310232 4.4:+37937884 None:intergenic
TCATCCACTCGTTCAGAATC+AGG 0.322426 4.4:+37933489 None:intergenic
CACAATACCCAAACAACTAT+TGG 0.328740 4.4:-37934094 MS.gene45686:CDS
ATTCCTGAGTTTGCAAACTT+TGG 0.335169 4.4:-37934338 MS.gene45686:CDS
GTGTGGCAATGCTTCTGTTC+TGG 0.335873 4.4:+37935705 None:intergenic
AATACTGTCATCACTCTTCT+TGG 0.336080 4.4:-37933426 MS.gene45686:CDS
TCATATGAATCTACAGTAGA+TGG 0.338705 4.4:+37934377 None:intergenic
TCAGGAAATATTACACAATC+AGG 0.339131 4.4:-37936235 MS.gene45686:CDS
CACCAACTGCAACATTGATA+TGG 0.343307 4.4:-37935744 MS.gene45686:CDS
ACATCTTCCAATAGTTGTTT+GGG 0.347400 4.4:+37934087 None:intergenic
CTCCACTTCCTTGGCATTGA+AGG 0.364156 4.4:-37935147 MS.gene45686:CDS
ACTGTTATACCTACTGAATC+AGG 0.365100 4.4:-37938125 MS.gene45686:CDS
ATGCCATTCGTGTGCTTTGT+CGG 0.366102 4.4:-37937686 MS.gene45686:CDS
AAATTTGGTGCTTCTGTTGA+TGG 0.368371 4.4:-37935024 MS.gene45686:CDS
TTCCTGAGTTTGCAAACTTT+GGG 0.370603 4.4:-37934337 MS.gene45686:CDS
AAGGCAATTACAGACCTTAA+TGG 0.375353 4.4:-37936139 MS.gene45686:CDS
TTTACCAGGGGCTTTCTTCT+CGG 0.376153 4.4:+37934439 None:intergenic
CAAATCCTGAGATTGTAAAA+AGG 0.381855 4.4:-37937463 MS.gene45686:CDS
AATATACCGAATATATTTGC+TGG 0.382125 4.4:+37935107 None:intergenic
GTGAGCACACTAGCCAAATT+TGG 0.386077 4.4:-37935039 MS.gene45686:CDS
TGATATCCCGCTCGTCAATC+TGG 0.391883 4.4:-37934620 MS.gene45686:CDS
TTTCAAATACCGCTCAATTT+GGG 0.393088 4.4:+37937641 None:intergenic
GGCTGAGACTTGACCTCCTT+GGG 0.395700 4.4:+37934467 None:intergenic
CCAGATGTTGGCAATCCCTC+AGG 0.396127 4.4:+37933870 None:intergenic
TAATTGCTGGAGTTAATTCC+CGG 0.397276 4.4:+37936107 None:intergenic
AGGGCCGCACTTGCAACAAC+TGG 0.399353 4.4:+37937597 None:intergenic
AACCCACTTTCCTTAGCATC+AGG 0.400667 4.4:+37935313 None:intergenic
CCCGGCTAGTAACACCATTA+AGG 0.408116 4.4:+37936125 None:intergenic
ATCTCGAGAATGCAACTGTC+AGG 0.412177 4.4:-37935071 MS.gene45686:CDS
CCTGAGGGATTGCCAACATC+TGG 0.415471 4.4:-37933870 MS.gene45686:CDS
AGCTGAAGAACAGTAATTGC+TGG 0.415694 4.4:+37936094 None:intergenic
TCTGAGATTTATTGTTAAAG+AGG 0.421358 4.4:-37933832 MS.gene45686:intron
AGGAAGAGATCTGAGGGGTT+TGG 0.422472 4.4:+37933932 None:intergenic
GGGAAGTGTTCGCTCACCTT+TGG 0.422783 4.4:-37936379 MS.gene45686:CDS
GCTTCGGACAAGCCTTCCCT+TGG 0.423385 4.4:+37933453 None:intergenic
TTAGAAAACTTTCCAGATGT+TGG 0.424560 4.4:+37933858 None:intergenic
CGCACTGGCTTTGCTACATC+AGG 0.427902 4.4:-37937384 MS.gene45686:intron
AATGGTGTACCTGATAATAA+AGG 0.432582 4.4:-37937893 MS.gene45686:CDS
GCTTTCAAGAAAATCATAGA+AGG 0.434619 4.4:+37936204 None:intergenic
TCTTTGACTGCAGGAGCCTT+CGG 0.438517 4.4:-37934516 MS.gene45686:intron
CTGCAGGTTATTCGTGAGTC+AGG 0.439768 4.4:-37936253 MS.gene45686:intron
AAGAAGGTTAACCTTGTTCA+AGG 0.440524 4.4:+37936024 None:intergenic
CTTCAGCCTCGCCAGAGGTT+GGG 0.441129 4.4:+37933622 None:intergenic
GGCTAGTGTGCTCACAGCAC+TGG 0.441877 4.4:+37935047 None:intergenic
ACAGCAGCAAATCTCAATAC+TGG 0.445031 4.4:+37936052 None:intergenic
AGGCTGAGACTTGACCTCCT+TGG 0.447016 4.4:+37934466 None:intergenic
GGGCTGTTCTTCAAGAGGCC+CGG 0.450990 4.4:-37938436 MS.gene45686:CDS
TATTGTCGGGTGTATACATA+GGG 0.451006 4.4:-37932302 MS.gene45686:CDS
TCTTCAGCCTCGCCAGAGGT+TGG 0.451809 4.4:+37933621 None:intergenic
ATTCCAGGTTATTGTAGTTG+TGG 0.452428 4.4:-37933991 MS.gene45686:intron
TTCCCAAAGTTTGCAAACTC+AGG 0.454897 4.4:+37934335 None:intergenic
CTTTATCTTAACACACTTGG+AGG 0.455687 4.4:-37934691 MS.gene45686:CDS
CCTTAGATACTCCACTTCCT+TGG 0.456772 4.4:-37935156 MS.gene45686:intron
AATTTGTAATCTGCTTCATA+AGG 0.458318 4.4:+37935633 None:intergenic
GTCCTTCAATGCCAAGGAAG+TGG 0.461397 4.4:+37935145 None:intergenic
TGGACCCCAGCAAATATATT+CGG 0.466206 4.4:-37935113 MS.gene45686:CDS
TTTCCTGAGTAACATTCTTA+GGG 0.469038 4.4:-37935401 MS.gene45686:CDS
GGCTGTTCTTCAAGAGGCCC+GGG 0.470531 4.4:-37938435 MS.gene45686:CDS
CTTCGGACAAGCCTTCCCTT+GGG 0.471166 4.4:+37933454 None:intergenic
CATGTGTGTGACCTTGAATT+AGG 0.473080 4.4:+37932327 None:intergenic
CGAGTGGATGAATACGGTCT+TGG 0.474887 4.4:-37933477 MS.gene45686:CDS
AGTCTCGAGATCTTGGGTTA+AGG 0.477809 4.4:-37937918 MS.gene45686:CDS
AAAGGATGTTGCAATGAAAC+TGG 0.479392 4.4:-37932235 MS.gene45686:CDS
CTTGATCCGTTATACAAGTC+AGG 0.479793 4.4:-37936346 MS.gene45686:intron
ACTGCAGGAGCCTTCGGAAG+AGG 0.482502 4.4:-37934510 MS.gene45686:intron
CCTTCGTCACAAGTCAGAAA+TGG 0.484353 4.4:-37936179 MS.gene45686:CDS
TCGTGTGCTTTGTCGGATCA+CGG 0.486313 4.4:-37937679 MS.gene45686:CDS
CCCGCATATTTGTTCTGCTA+AGG 0.488720 4.4:-37934889 MS.gene45686:CDS
ATAGTAAGACTGATATGTAC+AGG 0.489196 4.4:-37937713 MS.gene45686:CDS
ACCTTAATGGTGTTACTAGC+CGG 0.490430 4.4:-37936126 MS.gene45686:CDS
TTCCATATCAATGTTGCAGT+TGG 0.492232 4.4:+37935742 None:intergenic
AACACACCTGACTTGTATAA+CGG 0.494646 4.4:+37936340 None:intergenic
TTGCAGTTGGTGACTGCCAT+TGG 0.496069 4.4:+37935755 None:intergenic
ATCCCTGATGCTAAGGAAAG+TGG 0.499662 4.4:-37935316 MS.gene45686:CDS
TCTGCTTCAGTAAGCTCCAC+AGG 0.503537 4.4:+37934189 None:intergenic
GATGCGAGGGACTTCCTCTT+CGG 0.505539 4.4:-37934649 MS.gene45686:CDS
GACCAGGTTGACCCAACCTC+TGG 0.507760 4.4:-37933633 MS.gene45686:intron
GGCAGATTACATATTGAAAG+TGG 0.509324 4.4:-37933550 MS.gene45686:CDS
CATATGAATCTACAGTAGAT+GGG 0.511144 4.4:+37934378 None:intergenic
GTCACACACATGCTTATTGT+CGG 0.512785 4.4:-37932316 MS.gene45686:CDS
GATGACAGTATTTACAGCTT+CGG 0.513779 4.4:+37933437 None:intergenic
AACCGCTTGCCACAATCTCA+TGG 0.515317 4.4:+37932175 None:intergenic
AGTAACATTCTTAGGGAAGA+AGG 0.515461 4.4:-37935394 MS.gene45686:CDS
ACCGCTCAATTTGGGTAAGG+AGG 0.516223 4.4:+37937649 None:intergenic
TGACATTAATTTGGTGCCCA+AGG 0.517478 4.4:-37934483 MS.gene45686:CDS
GTCTGCCCAGGTGAATCATA+AGG 0.518091 4.4:+37933912 None:intergenic
AGATCTTGGGTTAAGGAGAA+TGG 0.521392 4.4:-37937911 MS.gene45686:CDS
ATATACCGAATATATTTGCT+GGG 0.521845 4.4:+37935108 None:intergenic
TCTTGTACAATTTCACGCAC+TGG 0.521922 4.4:-37937399 MS.gene45686:CDS
TTACCAGTCCTCAGCACCTG+TGG 0.522599 4.4:-37934205 MS.gene45686:intron
ACACTTTATCTTAACACACT+TGG 0.523186 4.4:-37934694 MS.gene45686:CDS
ATACTGGCTTGGAAGAACTT+AGG 0.525861 4.4:+37936068 None:intergenic
ATGAATTACCTTCGTCGTCA+CGG 0.525890 4.4:+37939132 None:intergenic
AGGTCGTCCCACCTAATTCA+AGG 0.529115 4.4:-37932338 MS.gene45686:CDS
CTTCCCTAAGAATGTTACTC+AGG 0.529688 4.4:+37935398 None:intergenic
TCTCTTCCTTATGATTCACC+TGG 0.529952 4.4:-37933918 MS.gene45686:CDS
CCTTAGCAGAACAAATATGC+GGG 0.530435 4.4:+37934889 None:intergenic
GGGGCTTTCTTCTCGGCAAG+AGG 0.532512 4.4:+37934446 None:intergenic
TAGAGATATCCCTGATGCTA+AGG 0.533771 4.4:-37935323 MS.gene45686:CDS
CAGGAAATATTACACAATCA+GGG 0.535239 4.4:-37936234 MS.gene45686:CDS
TACGGTCTTGGCCCAAGGGA+AGG 0.536329 4.4:-37933465 MS.gene45686:CDS
CCTTAATGGTGTTACTAGCC+GGG 0.540122 4.4:-37936125 MS.gene45686:CDS
TGGTCTCCAGATTGACGAGC+GGG 0.541433 4.4:+37934614 None:intergenic
TTAACCCAAGATCTCGAGAC+TGG 0.541890 4.4:+37937920 None:intergenic
AATACCGCTCAATTTGGGTA+AGG 0.542095 4.4:+37937646 None:intergenic
TTTATAACCTCGTCCGCAGA+GGG 0.542155 4.4:+37937862 None:intergenic
GCAAGTGCGGCCCTAGTTAG+TGG 0.543102 4.4:-37937588 MS.gene45686:CDS
AGCAAATCTCAATACTGGCT+TGG 0.543208 4.4:+37936057 None:intergenic
TCCCTGATGCTAAGGAAAGT+GGG 0.543834 4.4:-37935315 MS.gene45686:CDS
GTAGATGAATACCACTAACT+AGG 0.544395 4.4:+37937577 None:intergenic
TCTTGGCATGCAACCTTGTG+AGG 0.544482 4.4:-37933409 MS.gene45686:intron
TTATTGTCGGGTGTATACAT+AGG 0.544529 4.4:-37932303 MS.gene45686:CDS
CATCCACTCGTTCAGAATCA+GGG 0.547058 4.4:+37933490 None:intergenic
TCTGGCGAGGCTGAAGATGA+TGG 0.547600 4.4:-37933615 MS.gene45686:CDS
ATCTCGAGACTGGAAAAGCT+TGG 0.548683 4.4:+37937930 None:intergenic
TGCTGTCCGCACCTTGAACA+AGG 0.550630 4.4:-37936035 MS.gene45686:intron
ATCAAGAGACATTGGGCCAA+AGG 0.551616 4.4:+37936363 None:intergenic
CGTTGAAGATGAATACCAGC+TGG 0.552341 4.4:-37933592 MS.gene45686:CDS
TAGTGGTATTCATCTACTCC+AGG 0.552999 4.4:-37937571 MS.gene45686:intron
TCTGAACGAGTGGATGAATA+CGG 0.554007 4.4:-37933483 MS.gene45686:CDS
TCACACACATGCTTATTGTC+GGG 0.555717 4.4:-37932315 MS.gene45686:CDS
ATGAATACGGTCTTGGCCCA+AGG 0.555779 4.4:-37933470 MS.gene45686:CDS
TGTCTAATTTCAGGAACGCA+TGG 0.557299 4.4:-37933524 MS.gene45686:CDS
GCAGATTACATATTGAAAGT+GGG 0.557921 4.4:-37933549 MS.gene45686:CDS
AAATTATCACCTGTGTCGAC+AGG 0.558214 4.4:+37935248 None:intergenic
GTGCTTTGTCGGATCACGGA+TGG 0.561141 4.4:-37937675 MS.gene45686:CDS
ATGATCCACAACTAGATGCT+AGG 0.562098 4.4:-37938406 MS.gene45686:CDS
TATAACGGATCAAGAGACAT+TGG 0.565443 4.4:+37936355 None:intergenic
ATAACGGATCAAGAGACATT+GGG 0.567943 4.4:+37936356 None:intergenic
AGATGCTAGGAGATGTTCAC+AGG 0.568443 4.4:-37938393 MS.gene45686:intron
ATCATAAGGAAGAGATCTGA+GGG 0.568747 4.4:+37933926 None:intergenic
CCGCTCAATTTGGGTAAGGA+GGG 0.576163 4.4:+37937650 None:intergenic
CGTCACGAGCTCTCTCATGA+AGG 0.576960 4.4:-37937742 MS.gene45686:CDS
CATCATTGGCTTACCTCACA+AGG 0.578882 4.4:+37933396 None:intergenic
CAGGAACGCATGGGAAAGCA+TGG 0.579106 4.4:-37933514 MS.gene45686:CDS
AACGCCACAAAAGTCTGCCC+AGG 0.583006 4.4:+37933900 None:intergenic
TAGATGAATACCACTAACTA+GGG 0.583080 4.4:+37937578 None:intergenic
GAATCCAGTTGTTGCAAGTG+CGG 0.583487 4.4:-37937601 MS.gene45686:CDS
AAGAGTAGTAATAGCAAGTG+TGG 0.586121 4.4:+37935688 None:intergenic
TGAATACGGTCTTGGCCCAA+GGG 0.587422 4.4:-37933469 MS.gene45686:CDS
GGATATCAAGTGAGCCGAAG+AGG 0.589509 4.4:+37934635 None:intergenic
TCATCGTCTTTCTTGATAAG+CGG 0.590697 4.4:+37939155 None:intergenic
CTACTGTAGATTCATATGAA+AGG 0.592155 4.4:-37934373 MS.gene45686:CDS
GTGTGTGACCTTGAATTAGG+TGG 0.595130 4.4:+37932330 None:intergenic
AGCTGGTTACTAGCTTGACA+AGG 0.595888 4.4:-37936401 MS.gene45686:CDS
TATAACCTCGTCCGCAGAGG+GGG 0.598989 4.4:+37937864 None:intergenic
CAGATTACATATTGAAAGTG+GGG 0.600224 4.4:-37933548 MS.gene45686:CDS
TCGACTAGCAGTTAGCAAGC+TGG 0.600871 4.4:-37936418 MS.gene45686:CDS
CATCTCCTAGCATCTAGTTG+TGG 0.601531 4.4:+37938401 None:intergenic
GACAGGAAATGAATCAAGTG+TGG 0.601757 4.4:-37935660 MS.gene45686:CDS
GTCTAATTTCAGGAACGCAT+GGG 0.602050 4.4:-37933523 MS.gene45686:CDS
GACGATGACCGTGACGACGA+AGG 0.602361 4.4:-37939140 MS.gene45686:intron
TGAGATTGATAAAGATGCGA+GGG 0.603374 4.4:-37934662 MS.gene45686:CDS
ACCCACTTTCCTTAGCATCA+GGG 0.604088 4.4:+37935314 None:intergenic
TGTGGCGTTTGAGAAGCCTG+AGG 0.604943 4.4:-37933886 MS.gene45686:CDS
ATATGAATCTACAGTAGATG+GGG 0.607122 4.4:+37934379 None:intergenic
ATGTCAAAAGCCTCTTCCGA+AGG 0.609072 4.4:+37934500 None:intergenic
AGTAAGCTCCACAGGTGCTG+AGG 0.610022 4.4:+37934197 None:intergenic
GTGGCGTTTGAGAAGCCTGA+GGG 0.610662 4.4:-37933885 MS.gene45686:CDS
ATCATCTTCAGCCTCGCCAG+AGG 0.614172 4.4:+37933617 None:intergenic
AGACTGGAAAAGCTTGGTAA+CGG 0.615810 4.4:+37937936 None:intergenic
ATGTCTCTCCCTGATTCAGT+AGG 0.615879 4.4:+37938116 None:intergenic
GATCCGACAAAGCACACGAA+TGG 0.616477 4.4:+37937683 None:intergenic
CTGTTATACCTACTGAATCA+GGG 0.617217 4.4:-37938124 MS.gene45686:CDS
CTCTTCCTTATGATTCACCT+GGG 0.617576 4.4:-37933917 MS.gene45686:CDS
CATTAATTTGGTGCCCAAGG+AGG 0.617595 4.4:-37934480 MS.gene45686:CDS
GCTTGCCACAATCTCATGGA+TGG 0.618369 4.4:+37932179 None:intergenic
CCATTTCTGACTTGTGACGA+AGG 0.619492 4.4:+37936179 None:intergenic
CTTGCCGAGAAGAAAGCCCC+TGG 0.619544 4.4:-37934443 MS.gene45686:CDS
ATACCAGCTGGAGGATCTAG+AGG 0.621534 4.4:-37933580 MS.gene45686:CDS
GGTTGACCCAACCTCTGGCG+AGG 0.622975 4.4:-37933628 MS.gene45686:CDS
CAACCTCTAGATCCTCCAGC+TGG 0.624747 4.4:+37933577 None:intergenic
AGGAAATATTACACAATCAG+GGG 0.628089 4.4:-37936233 MS.gene45686:CDS
ATCCATGAGATTGTGGCAAG+CGG 0.630120 4.4:-37932177 MS.gene45686:CDS
TGATGCCATCCATGAGATTG+TGG 0.630840 4.4:-37932184 MS.gene45686:CDS
AACATTCTTAGGGAAGAAGG+TGG 0.632595 4.4:-37935391 MS.gene45686:CDS
GGTTAACCTTGTTCAAGGTG+CGG 0.637434 4.4:+37936029 None:intergenic
AATCATAAGGAAGAGATCTG+AGG 0.638595 4.4:+37933925 None:intergenic
TCTCGAGAATGCAACTGTCA+GGG 0.639017 4.4:-37935070 MS.gene45686:CDS
GGAGGATCTAGAGGTTGTTG+CGG 0.641056 4.4:-37933571 MS.gene45686:CDS
GCCTTAGCAGAACAAATATG+CGG 0.646975 4.4:+37934888 None:intergenic
AAGTGTGTTAAGATAAAGTG+TGG 0.647393 4.4:+37934696 None:intergenic
ATTTATAACCTCGTCCGCAG+AGG 0.647754 4.4:+37937861 None:intergenic
CTGAGATTGATAAAGATGCG+AGG 0.648808 4.4:-37934663 MS.gene45686:CDS
GGAGCACCCAAACCACCAGT+TGG 0.649439 4.4:+37934413 None:intergenic
TTGTCGGGTGTATACATAGG+GGG 0.654756 4.4:-37932300 MS.gene45686:CDS
AACGGAAAAGAAAACTTCTG+TGG 0.658504 4.4:+37937954 None:intergenic
GGACCCTGATTCTGAACGAG+TGG 0.660047 4.4:-37933493 MS.gene45686:CDS
CTGGTTACTAGCTTGACAAG+GGG 0.663034 4.4:-37936399 MS.gene45686:CDS
CTTTCAAGAAAATCATAGAA+GGG 0.664990 4.4:+37936205 None:intergenic
TAGTAAGACTGATATGTACA+GGG 0.670523 4.4:-37937712 MS.gene45686:CDS
TGTGTGACCTTGAATTAGGT+GGG 0.672239 4.4:+37932331 None:intergenic
GCTGGTTACTAGCTTGACAA+GGG 0.673610 4.4:-37936400 MS.gene45686:CDS
ATTCACTTACCTGTCGACAC+AGG 0.673693 4.4:-37935257 MS.gene45686:intron
GGGGTGTGAAGGTACTTGTG+CGG 0.674790 4.4:-37932281 MS.gene45686:CDS
GGTGGCAATGACACATCCAA+TGG 0.675268 4.4:-37935771 MS.gene45686:CDS
AGATTACATATTGAAAGTGG+GGG 0.675495 4.4:-37933547 MS.gene45686:CDS
CCCTCCTTACCCAAATTGAG+CGG 0.675546 4.4:-37937650 MS.gene45686:CDS
TGAAGATGAATACCAGCTGG+AGG 0.678607 4.4:-37933589 MS.gene45686:CDS
CTTAGCAGAACAAATATGCG+GGG 0.682439 4.4:+37934890 None:intergenic
TCAGGGAGAGACATTTACAA+AGG 0.683582 4.4:-37938107 MS.gene45686:intron
TATACCGAATATATTTGCTG+GGG 0.697705 4.4:+37935109 None:intergenic
CTGGTCTCCAGATTGACGAG+CGG 0.703175 4.4:+37934613 None:intergenic
TATGAATCTACAGTAGATGG+GGG 0.704989 4.4:+37934380 None:intergenic
ATTGTCGGGTGTATACATAG+GGG 0.715775 4.4:-37932301 MS.gene45686:CDS
TCACTACTTGCAGGGCACAG+AGG 0.740479 4.4:-37932358 MS.gene45686:intron
TTATAACCTCGTCCGCAGAG+GGG 0.751797 4.4:+37937863 None:intergenic
TCATAAGGAAGAGATCTGAG+GGG 0.799654 4.4:+37933927 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.4 gene 37932173 37939186 37932173 ID=MS.gene45686
chr4.4 mRNA 37932173 37939186 37932173 ID=MS.gene45686.t1;Parent=MS.gene45686
chr4.4 exon 37939141 37939186 37939141 ID=MS.gene45686.t1.exon1;Parent=MS.gene45686.t1
chr4.4 CDS 37939141 37939186 37939141 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37938394 37938491 37938394 ID=MS.gene45686.t1.exon2;Parent=MS.gene45686.t1
chr4.4 CDS 37938394 37938491 37938394 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37938108 37938158 37938108 ID=MS.gene45686.t1.exon3;Parent=MS.gene45686.t1
chr4.4 CDS 37938108 37938158 37938108 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37937870 37937983 37937870 ID=MS.gene45686.t1.exon4;Parent=MS.gene45686.t1
chr4.4 CDS 37937870 37937983 37937870 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37937572 37937772 37937572 ID=MS.gene45686.t1.exon5;Parent=MS.gene45686.t1
chr4.4 CDS 37937572 37937772 37937572 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37937385 37937486 37937385 ID=MS.gene45686.t1.exon6;Parent=MS.gene45686.t1
chr4.4 CDS 37937385 37937486 37937385 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37936347 37936454 37936347 ID=MS.gene45686.t1.exon7;Parent=MS.gene45686.t1
chr4.4 CDS 37936347 37936454 37936347 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37936036 37936269 37936036 ID=MS.gene45686.t1.exon8;Parent=MS.gene45686.t1
chr4.4 CDS 37936036 37936269 37936036 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37935542 37935792 37935542 ID=MS.gene45686.t1.exon9;Parent=MS.gene45686.t1
chr4.4 CDS 37935542 37935792 37935542 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37935258 37935429 37935258 ID=MS.gene45686.t1.exon10;Parent=MS.gene45686.t1
chr4.4 CDS 37935258 37935429 37935258 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37935017 37935172 37935017 ID=MS.gene45686.t1.exon11;Parent=MS.gene45686.t1
chr4.4 CDS 37935017 37935172 37935017 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37934862 37934912 37934862 ID=MS.gene45686.t1.exon12;Parent=MS.gene45686.t1
chr4.4 CDS 37934862 37934912 37934862 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37934600 37934731 37934600 ID=MS.gene45686.t1.exon13;Parent=MS.gene45686.t1
chr4.4 CDS 37934600 37934731 37934600 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37934325 37934525 37934325 ID=MS.gene45686.t1.exon14;Parent=MS.gene45686.t1
chr4.4 CDS 37934325 37934525 37934325 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37934089 37934220 37934089 ID=MS.gene45686.t1.exon15;Parent=MS.gene45686.t1
chr4.4 CDS 37934089 37934220 37934089 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37933833 37934006 37933833 ID=MS.gene45686.t1.exon16;Parent=MS.gene45686.t1
chr4.4 CDS 37933833 37934006 37933833 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37933410 37933649 37933410 ID=MS.gene45686.t1.exon17;Parent=MS.gene45686.t1
chr4.4 CDS 37933410 37933649 37933410 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
chr4.4 exon 37932173 37932367 37932173 ID=MS.gene45686.t1.exon18;Parent=MS.gene45686.t1
chr4.4 CDS 37932173 37932367 37932173 ID=cds.MS.gene45686.t1;Parent=MS.gene45686.t1
Gene Sequence

>MS.gene45686

ATGGCTCAGCCGCTTATCAAGAAAGACGATGACCGTGACGACGAAGGTAATTCATTCTTCCCACTTTTTCCGTTTTTCTCTTTCGCGCAATTCGATCTACTCTCTTCCGTGCTCGTTTTCTAGATCCAATGCTTAATTTCTGTTTCTATTGAGGAATGCGGTACGGTTCGCGTAGATCACGATGCTTGAATTGAATTGGATTCGATTAGTTCGTTTTTTAATTGTTGTCGGTTTGTAGAAATTTGAGTTGATCTAATCGATAATCATGTAATTTGAAATGCAGTTTATTATTAATAACGTGCAATTGCTTGTGTGAATTTGAACAAGTTGATTAATAGCTAATTTTGATATTTTATAGCTTCTTTTCACACTTTGGTCACGATTAGTGCGTTTTTTGAATTGTTTTATATGACTTTTGTTTGTAGTGATTTGTAGAAATTAGAAAATTGTTTTGATCTAGCTATGTAATTTGAAATGCAGTTCTTTTGTCAGGTGCAATTGCTTGTTTGAATTTATCACAACTTCACAAGTTTATGGTGACTAGTTAGTTGGTTAATAGAATATTTAGATATTTTATAGCTTTTTTTCAAACTTTGGTTAGGACTCAAGCTGGTTTATTGTGATGAGTCCTACATACATATATTATAATGTCAATATTTGAGGATTAATTATGGTTTTTAATTTTTGTTTAACAGATAATTATTCCCCGTTTTTGGGAATTGAAAAGGGGGCTGTTCTTCAAGAGGCCCGGGTTTTTAATGATCCACAACTAGATGCTAGGAGATGTTCACAGGTGCTTATATTTATTTATTTGTTTATTTATCTATGGTTTCACTGTCTGAGCCAATGAAGCACTGACACAGCACGACACGGTCGACACTTGTACTAATTTGATAAAAAACAAATTAATCTAACGTAACCACGTGTCGGAGTTTGACATTGAAATGCCATCAATTAGAGGTGTTGGTTTTACAAAGGTCTTGATCAGTGGTTGATTTTTGAAAGTGTGTATGTTTATCTTTTTCCAGGTTATCACAAAACTGTTATACCTACTGAATCAGGGAGAGACATTTACAAAGGTCAAGATGTTTTCCTTTTATTTCTTGAGTAAAGATTATATCTTGCTTATAGATGGAATTTGCTGGATTGCGTTAGTCAAAATGAGTCCTTTTTGAGTAAATTTTTCTTGCCACATTTCTACAGGTTGAAGCCACAGAAGTTTTCTTTTCCGTTACCAAGCTTTTCCAGTCTCGAGATCTTGGGTTAAGGAGAATGGTGTACCTGATAATAAAGGAACTCTCCCCCTCTGCGGACGAGGTTATAAATTTTCATGCAATTTATCTTGTTAGATTCACTTAAACGGCCTGTGTTTTACTAAATTCTGACTGTTCCATCTCTTTTATTTTTTTAAAAGGTTATTATCGTCACGAGCTCTCTCATGAAGGACATGAATAGTAAGACTGATATGTACAGGGCAAATGCCATTCGTGTGCTTTGTCGGATCACGGATGGAACCCTCCTTACCCAAATTGAGCGGTATTTGAAACAAGCAATTGTAGACAAGAATCCAGTTGTTGCAAGTGCGGCCCTAGTTAGTGGTATTCATCTACTCCAGGTATTATTAATATATATTTGTAATCCTTCTATGATCTGTATATTTCATCTACATCAGTTGCTTATTGTATATCCCCAACTTTCAGACAAATCCTGAGATTGTAAAAAGGTGGAGCAATGAGGTTCAAGAAGCTGTACAATCAAGAGCAGCTCTTGTACAATTTCACGCACTGGCTTTGCTACATCAGGTATGCAGTGTGAATCTTTGATGATGAAAGCCTTCTTTGTGTATTAAAATATATTTTCTTGAATAGCCTAATTTGTCAGTGATGCAATGTAATTCATCAGTTGCTGGGATATGTAACAAGATCAATTTCTTGCACGGTCAATTTTTCGGTTCATCACAGTTGAAGTTCATTGAATATTTAGCATGAGCTAGTGTTTTATTATTTCCTTTCAAAACCTGTTACATATTCAGCATTACATGATATCGAAGCTTTATGGTCAAGTGGTTTTAGACTTCAGTGTTAAATTCTTTTGACCTCCATCTTTCCAAATAAAAATTCAAATTCGGTACAAGGGGATGTTAAATATGAAAACCATAATATGCCTTCAGTGGACAACTTGAGCTTTTTTAATTATTGGTTCGTGTCAAAAATCAATTAACTTTTGTCTCATTTTTCTATAAGCTTCTCAAGCAAATGAATTTACCACAATAAAAGAAAGCAAAACTCCTTGTAATACAACCAAAGAAGTTGATATTTGATTATTTGGTCTCTACTCCATCTTATGACATTATGCCATCTATTAATTTAGCATACTCCTATTCATACTCTTATTTTAGCTTCAATTACAGCTGCAATCGATAACTGGCTGCATCGTATAGCACTGTGAATAGTTTTAAAGACTGATTATCTTGCTGTGTTCCCAACTTTGGCAAAGTCTGTATATGTATAAACCTGTCTGCATTTTTTGTACATCATTGTCTTCTGAAGTTACTTTGAACATGGCCATGATGGAAATTCTATTCTATCGAATCAATTCATATCACTCTTAAAGTCCTATCAATGACACGTTGATAAGTTAATTATACATTCCAAAAATCCTTCTTTTATTGGCATACCAAACAAATAAGTTCAAGCATATTTGTTGACACATGGTAACTTGCAACATTGCAGATACGGCAGAATGATCGACTAGCAGTTAGCAAGCTGGTTACTAGCTTGACAAGGGGAAGTGTTCGCTCACCTTTGGCCCAATGTCTCTTGATCCGTTATACAAGTCAGGTGTGTTTTCCTTTTCTTATAGAGTTGTCCGTGGGAGATATTCATTTGAATAATTTTATGTAGTATAATATCTGCAGGTTATTCGTGAGTCAGGAAATATTACACAATCAGGGGAACGACCCTTCTATGATTTTCTTGAAAGCTGCCTTCGTCACAAGTCAGAAATGGTGATTTTTGAAGCTGCTAAGGCAATTACAGACCTTAATGGTGTTACTAGCCGGGAATTAACTCCAGCAATTACTGTTCTTCAGCTCTTCCTAAGTTCTTCCAAGCCAGTATTGAGATTTGCTGCTGTCCGCACCTTGAACAAGGTTAACCTTCTTTAATCACATAAATTTCAGTAGAAGTACTGTTAATGCTTTCATAAAATTTGAGAAAGGATATATGTTTGATTCTCTCCCTTTTGGAGGCACATTTTGCTTTTCTGTCTGATATGATTTTTGAAAAATTGATGAAAAAAATTATTGTATTGTAGCTAATATTAAAAGATTTTTTATGGTCTAACTTTCAATGTTTTTATTAATAATTTATGAGCGTGGCTTTGAACTTTGCAGGTGGCAATGACACATCCAATGGCAGTCACCAACTGCAACATTGATATGGAAAGTTTAATCTCTGACCAGAACAGAAGCATTGCCACACTTGCTATTACTACTCTTTTGAAGACAGGAAATGAATCAAGTGTGGATCGCCTTATGAAGCAGATTACAAATTTCATGTCTGATATTGCTGACGAGTTCAAAATTGTTGTTGTTGAAGCAATAAGATCATTGTGCTTGAAGTTTCCTTTAAAATATAGATCTCTGTGAGTATATGTGTCATTCTCTATGTTTCTTTCAGCCAAATGTTACTTCTTTCCTATATTTTCCCTGATAAATGGTCTAATTTACTGGTTTCTATTATGTTATTATTTTCAGGATGAATTTCCTGAGTAACATTCTTAGGGAAGAAGGTGGTTTCGATTACAAGAAAGCAATTGTCGATTCAATAGTGATTCTTATTAGAGATATCCCTGATGCTAAGGAAAGTGGGTTACTTCATCTTTGTGAGTTCATTGAAGATTGTGAATTCACTTACCTGTCGACACAGGTGATAATTTTGGTTGTGCTTTGTAGTTTACTTTGCGTGATATTGGAATTTTACCTCTTGTTAATTTTTTCCCCTTGTACCTTAGATACTCCACTTCCTTGGCATTGAAGGACCAAAAACATTGGACCCCAGCAAATATATTCGGTATATTTATAACAGAGTACATCTCGAGAATGCAACTGTCAGGGCCAGTGCTGTGAGCACACTAGCCAAATTTGGTGCTTCTGTTGATGGATTAAAGGTGCTGTTGCCTTTATTTATTAATTATTTTCTCTAGAATTTTTGTTTATTTAGGGTGATATTTGTTATTAACTTGATTAAAAATCTCTTAATTACTCTTTTCAGCCCCGCATATTTGTTCTGCTAAGGCGATGTCTTTTTGACAGTGATGACGAGGTCAACTTCTGTCATATCTTACATTGCATTTCTATAATTTATGTGGTTCCTACGATTTTCAGGGAAGTTTATCAAATTATTATTTGCTTTGATTTATATTTTGATGGTTATGATTTTGATTTCTTTGCAGGTTCGTGATAGAGCCACACTTTATCTTAACACACTTGGAGGTGATACTGAGATTGATAAAGATGCGAGGGACTTCCTCTTCGGCTCACTTGATATCCCGCTCGTCAATCTGGAGACCAGTTTGAAAAAATACGTAAGTTCTGTATATTGTTATTTCTTTTCATTAGTTACTTTAGGACATATCTCATGTTTCCTCTTTGACTGCAGGAGCCTTCGGAAGAGGCTTTTGACATTAATTTGGTGCCCAAGGAGGTCAAGTCTCAGCCTCTTGCCGAGAAGAAAGCCCCTGGTAAAAAGCCAACTGGTGGTTTGGGTGCTCCTCCTATTGGGCCCCCATCTACTGTAGATTCATATGAAAGGCAACTTCTGTCAATTCCTGAGTTTGCAAACTTTGGGAAGCTTTTTAAGGTTCTTTATCTAAATCTTACAAAATTTCTATATATTCGTATTGTTCTTTCTATATCCGATCTAATCATATGTATTCTGATATTATATATTATATTTCTTACCAGTCCTCAGCACCTGTGGAGCTTACTGAAGCAGAGACAGAATATGCAGTTAATGTTGTTAAACACATTTTTGATACACATGTTGTGTTTCAGTACAACTGCACAAACACAATACCCAAACAACTATTGGAAGATGTAATGACTAATATAGATGATGTACAATTTCATACTATTATGCAACTTCAGGCCATCTGTGTAATATTTTTCCCTATTCCAGGTTATTGTAGTTGTGGATGCTTCAGAAGCAGAAGAATTTTCTCAGGTGATCTCCAAACCCCTCAGATCTCTTCCTTATGATTCACCTGGGCAGACTTTTGTGGCGTTTGAGAAGCCTGAGGGATTGCCAACATCTGGAAAGTTTTCTAACATTCTGAGATTTATTGTTAAAGAGGTACTTGTTCCCTTTGTTTGTGGTTTTTCTGTCCAAGATCTCCATTATATTATGTATGCGTGTGTTTAAATGTCAATCATGAAGCTTATTGTGTTTTTTTCATCTTGAAATTTGTGCAAAACTAGACCTATAATAGTTTTTGTGGAAAGTTGTTCGTTACTTCATAACTTGCATATTGACCAGGTTGACCCAACCTCTGGCGAGGCTGAAGATGATGGCGTTGAAGATGAATACCAGCTGGAGGATCTAGAGGTTGTTGCGGCAGATTACATATTGAAAGTGGGGGTGTCTAATTTCAGGAACGCATGGGAAAGCATGGACCCTGATTCTGAACGAGTGGATGAATACGGTCTTGGCCCAAGGGAAGGCTTGTCCGAAGCTGTAAATACTGTCATCACTCTTCTTGGCATGCAACCTTGTGAGGTAAGCCAATGATGTCAGTTGTGGCTTCTTAAATGCTTGTTGTTATGAGACCCATTTTATTAAACTGATCATGAGTGTTTTGCACTATTATTACTGCAACTACAACATGAGGTGATGTCCTCCCAATTAGATTCTTTCCAGAAAACTAAAAAAATGTTACTATCAGGATTCATTTTCCATACACTGATTTCGTATTCATTTTCCATGATGGGCGTTCACTTTCTCATACATCAATTTTAGGCTGAACATTATTCTGAGATGTATATAAATACTTCAATGCTTGTAGCCAGTCCGCTTAGTTCCTTTTAAAATTTTGAAGTTGATCTTCGACACATTGCTAAACTCAAGCCACAAGGACGCCCATGAGTAGCATTTAAACTTTAAAGTGCTGTTCTTTGGACAGAATGCTATTCTGTGAACATGTGCTTGCACACAGGTTCATTGACAGATAACATGAAGTAGAAGAGATTCTCTGGTGCTTCAGGAGTAAGACATTTCTTTTCCCATGGCACAAATATCAATCCCAATTGTTGAGAGTTTTGATATACCTACATAAAGAGATGCAAATACATTTAGGCTATAGAAAATGTTTGCTTTAAGTCAGTAGTTCCTTTTAATGATTTGGCATTAGAGCTTTTTGACATTTTGTCAAATCCCTGCCACGACGAGGTTATAGATGCAAGTAAAAGCCTGTGTAAGCATAGACCATTGAGAACCCGATTTAAGAGTTTCTTAGGTTCTCGTGAGAGTAATGGATTTCTCTTTCACTTCATGGCCACTCGAGATAAAGTATTGGCTGATGTCATAGCTAACATCATCTGGAACCTGGAATCTTAGACATTTTGTTCTCTAACTCCATGCTAGTACCACCAAAGAACTTTTGAAAGAAAGGAGTAGAGCATCTTGTATCGATCAATGCATGTTCATTAGTAATACAACCGTATCTTCTAGGAAGGCAATATCTTTCTCATTATTTGTAATACAACCGTATCTACTAGGAAGGCAATATCTTTCTCATTATTTTATTGTTCACTACTTGCAGGGCACAGAGGTCGTCCCACCTAATTCAAGGTCACACACATGCTTATTGTCGGGTGTATACATAGGGGGTGTGAAGGTACTTGTGCGGCTGTCTTTTGGACTTGATGGTGCAAAGGATGTTGCAATGAAACTGGCTGTCAGATCTGATGATGTAACTGTCAGTGATGCCATCCATGAGATTGTGGCAAGCGGTTAG
Protein sequence

>MS.gene45686.t1

MAQPLIKKDDDRDDEDNYSPFLGIEKGAVLQEARVFNDPQLDARRCSQVITKLLYLLNQGETFTKVEATEVFFSVTKLFQSRDLGLRRMVYLIIKELSPSADEVIIVTSSLMKDMNSKTDMYRANAIRVLCRITDGTLLTQIERYLKQAIVDKNPVVASAALVSGIHLLQTNPEIVKRWSNEVQEAVQSRAALVQFHALALLHQIRQNDRLAVSKLVTSLTRGSVRSPLAQCLLIRYTSQVIRESGNITQSGERPFYDFLESCLRHKSEMVIFEAAKAITDLNGVTSRELTPAITVLQLFLSSSKPVLRFAAVRTLNKVAMTHPMAVTNCNIDMESLISDQNRSIATLAITTLLKTGNESSVDRLMKQITNFMSDIADEFKIVVVEAIRSLCLKFPLKYRSLMNFLSNILREEGGFDYKKAIVDSIVILIRDIPDAKESGLLHLCEFIEDCEFTYLSTQILHFLGIEGPKTLDPSKYIRYIYNRVHLENATVRASAVSTLAKFGASVDGLKPRIFVLLRRCLFDSDDEVRDRATLYLNTLGGDTEIDKDARDFLFGSLDIPLVNLETSLKKYEPSEEAFDINLVPKEVKSQPLAEKKAPGKKPTGGLGAPPIGPPSTVDSYERQLLSIPEFANFGKLFKSSAPVELTEAETEYAVNVVKHIFDTHVVFQYNCTNTIPKQLLEDVIVVVDASEAEEFSQVISKPLRSLPYDSPGQTFVAFEKPEGLPTSGKFSNILRFIVKEVDPTSGEAEDDGVEDEYQLEDLEVVAADYILKVGVSNFRNAWESMDPDSERVDEYGLGPREGLSEAVNTVITLLGMQPCEGTEVVPPNSRSHTCLLSGVYIGGVKVLVRLSFGLDGAKDVAMKLAVRSDDVTVSDAIHEIVASG