Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45794.t1 | AAK12936.1 | 55.8 | 86 | 31 | 3 | 16 | 99 | 64 | 144 | 2.90E-08 | 68.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45794.t1 | Q9AXJ6 | 55.8 | 86 | 31 | 3 | 16 | 99 | 64 | 144 | 2.1e-08 | 68.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene45794 | 0.823817 | 1.13E-53 | -1.69E-46 |
| MS.gene049701 | MS.gene45794 | 0.831167 | 1.95E-55 | -1.69E-46 |
| MS.gene049853 | MS.gene45794 | 0.835458 | 1.66E-56 | -1.69E-46 |
| MS.gene050611 | MS.gene45794 | 0.805825 | 1.11E-49 | -1.69E-46 |
| MS.gene051710 | MS.gene45794 | 0.804533 | 2.06E-49 | -1.69E-46 |
| MS.gene051932 | MS.gene45794 | 0.809683 | 1.68E-50 | -1.69E-46 |
| MS.gene052447 | MS.gene45794 | 0.830616 | 2.66E-55 | -1.69E-46 |
| MS.gene053512 | MS.gene45794 | 0.806675 | 7.33E-50 | -1.69E-46 |
| MS.gene053974 | MS.gene45794 | 0.805672 | 1.19E-49 | -1.69E-46 |
| MS.gene054174 | MS.gene45794 | 0.844056 | 9.57E-59 | -1.69E-46 |
| MS.gene054175 | MS.gene45794 | 0.847848 | 8.92E-60 | -1.69E-46 |
| MS.gene05435 | MS.gene45794 | 0.817194 | 3.74E-52 | -1.69E-46 |
| MS.gene054484 | MS.gene45794 | 0.836223 | 1.06E-56 | -1.69E-46 |
| MS.gene055056 | MS.gene45794 | 0.847519 | 1.10E-59 | -1.69E-46 |
| MS.gene055058 | MS.gene45794 | 0.813916 | 2.01E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene45794 | 0.805068 | 1.60E-49 | -1.69E-46 |
| MS.gene05762 | MS.gene45794 | 0.802496 | 5.46E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene45794 | 0.836915 | 7.07E-57 | -1.69E-46 |
| MS.gene05925 | MS.gene45794 | 0.808576 | 2.89E-50 | -1.69E-46 |
| MS.gene059276 | MS.gene45794 | 0.844606 | 6.81E-59 | -1.69E-46 |
| MS.gene059458 | MS.gene45794 | 0.801411 | 9.12E-49 | -1.69E-46 |
| MS.gene059460 | MS.gene45794 | 0.803026 | 4.24E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene45794 | 0.803542 | 3.32E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45794.t1 | MTR_5g085870 | 83.750 | 80 | 13 | 0 | 20 | 99 | 70 | 149 | 7.95e-28 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCGAACTCGTAAGGGGTTT+AGG | 0.258839 | 4.4:+76887523 | None:intergenic |
| GTGAAACGATATCAAGATTA+TGG | 0.295295 | 4.4:-76887312 | MS.gene45794:CDS |
| TCGAACTCGTAAGGGGTTTA+GGG | 0.305117 | 4.4:+76887524 | None:intergenic |
| TTTCTTACCAATCGACCTTT+GGG | 0.371081 | 4.4:+76887380 | None:intergenic |
| TGTGACAGCGATTGCGAAAG+AGG | 0.387116 | 4.4:+76887435 | None:intergenic |
| AGAGAATCGGAAGGTGAAAC+AGG | 0.391069 | 4.4:+76887486 | None:intergenic |
| AGTGTATGGTTGTAGGTTGC+TGG | 0.451119 | 4.4:-76886513 | MS.gene45794:intron |
| TGTTAGAAGTGTATGGTTGT+AGG | 0.463926 | 4.4:-76886520 | MS.gene45794:CDS |
| AAATGTTAGCAAGGATGTGT+TGG | 0.480699 | 4.4:-76886334 | MS.gene45794:CDS |
| AATGTTAGCAAGGATGTGTT+GGG | 0.487842 | 4.4:-76886333 | MS.gene45794:CDS |
| ATTGGTAAGAAAAGCGGCTC+TGG | 0.495571 | 4.4:-76887369 | MS.gene45794:CDS |
| TGTGTTGGGTTCTACTATTG+AGG | 0.496016 | 4.4:-76886319 | MS.gene45794:intron |
| CCCCTTAGAAAATGTTAGCA+AGG | 0.500090 | 4.4:-76886343 | MS.gene45794:CDS |
| ATATCAATGTTAGAAGTGTA+TGG | 0.508506 | 4.4:-76886527 | MS.gene45794:CDS |
| AAAATCACGTGAAAGAGAAT+CGG | 0.520635 | 4.4:+76887473 | None:intergenic |
| CTTCGATCCCAAAGGTCGAT+TGG | 0.530093 | 4.4:-76887387 | MS.gene45794:CDS |
| AGAGAGAAATCGAACTCGTA+AGG | 0.532543 | 4.4:+76887515 | None:intergenic |
| GAGAGAAATCGAACTCGTAA+GGG | 0.554082 | 4.4:+76887516 | None:intergenic |
| AGAAAAGCGGCTCTGGTAGT+CGG | 0.568712 | 4.4:-76887362 | MS.gene45794:CDS |
| GCGAAAGAGGAGAGTCATCA+TGG | 0.570240 | 4.4:+76887448 | None:intergenic |
| GCTCTGGTAGTCGGCAAAGT+GGG | 0.580582 | 4.4:-76887353 | MS.gene45794:CDS |
| GGCTCTGGTAGTCGGCAAAG+TGG | 0.629147 | 4.4:-76887354 | MS.gene45794:CDS |
| ATTACGCTGGTGGTCAGAAG+AGG | 0.632700 | 4.4:-76887263 | MS.gene45794:intron |
| AGGTCGATTGGTAAGAAAAG+CGG | 0.633867 | 4.4:-76887375 | MS.gene45794:CDS |
| AGAGAAATCGAACTCGTAAG+GGG | 0.643399 | 4.4:+76887517 | None:intergenic |
| TCGCAAGAGTATTACGCTGG+TGG | 0.647638 | 4.4:-76887273 | MS.gene45794:CDS |
| TGTATAAGCTTCGATCCCAA+AGG | 0.651826 | 4.4:-76887395 | MS.gene45794:CDS |
| GAATCGCAAGAGTATTACGC+TGG | 0.665497 | 4.4:-76887276 | MS.gene45794:CDS |
| TCACGTGAAAGAGAATCGGA+AGG | 0.687567 | 4.4:+76887477 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGATTAATTGTAATATT+AGG | - | chr4.4:76886430-76886449 | MS.gene45794:intron | 10.0% |
| !! | TTAATCTATAAAATTATTGA+TGG | - | chr4.4:76886662-76886681 | MS.gene45794:intron | 10.0% |
| !!! | ATTCATTGATTTAAATAATT+TGG | - | chr4.4:76886637-76886656 | MS.gene45794:intron | 10.0% |
| !! | GTAAAAATTAAAGAAGAAAT+GGG | - | chr4.4:76887208-76887227 | MS.gene45794:intron | 15.0% |
| !! | TGTAAAAATTAAAGAAGAAA+TGG | - | chr4.4:76887207-76887226 | MS.gene45794:intron | 15.0% |
| !! | TTAATTGTAATATTAGGTTT+AGG | - | chr4.4:76886436-76886455 | MS.gene45794:intron | 15.0% |
| !! | TTAGATATAGATTAACTAAA+AGG | + | chr4.4:76887392-76887411 | None:intergenic | 15.0% |
| !! | TTTAGTTAATCTATATCTAA+TGG | - | chr4.4:76887392-76887411 | MS.gene45794:CDS | 15.0% |
| !!! | ATTGATTTTGATTTTGATTA+TGG | - | chr4.4:76886362-76886381 | MS.gene45794:CDS | 15.0% |
| !!! | TTATCTGTTATTTGTTATTT+GGG | - | chr4.4:76886718-76886737 | MS.gene45794:intron | 15.0% |
| !!! | TTTATCTGTTATTTGTTATT+TGG | - | chr4.4:76886717-76886736 | MS.gene45794:intron | 15.0% |
| !! | GAAACTTTAACTAATAGATT+TGG | - | chr4.4:76886979-76886998 | MS.gene45794:intron | 20.0% |
| !!! | AAGTTACATATTTTTGTTGT+TGG | - | chr4.4:76887431-76887450 | MS.gene45794:CDS | 20.0% |
| !!! | AGTTACATATTTTTGTTGTT+GGG | - | chr4.4:76887432-76887451 | MS.gene45794:CDS | 20.0% |
| ! | AAATCAATCAACTAACACTA+AGG | + | chr4.4:76886932-76886951 | None:intergenic | 25.0% |
| ! | AGTTCCTTCATATTTCAATT+AGG | - | chr4.4:76886581-76886600 | MS.gene45794:intron | 25.0% |
| ! | ATGAGAACAATTACACAATA+AGG | + | chr4.4:76887518-76887537 | None:intergenic | 25.0% |
| ! | GAAACCTAATTGAAATATGA+AGG | + | chr4.4:76886588-76886607 | None:intergenic | 25.0% |
| !! | ATATCAATGTTAGAAGTGTA+TGG | - | chr4.4:76887050-76887069 | MS.gene45794:intron | 25.0% |
| !!! | ACTTAGGGTTTTAAGTTTAT+AGG | - | chr4.4:76886399-76886418 | MS.gene45794:intron | 25.0% |
| !!! | CTTAGGGTTTTAAGTTTATA+GGG | - | chr4.4:76886400-76886419 | MS.gene45794:intron | 25.0% |
| AAAATCACGTGAAAGAGAAT+CGG | + | chr4.4:76886107-76886126 | None:intergenic | 30.0% | |
| AATTGTTCTCATTGTTGTGT+AGG | - | chr4.4:76887526-76887545 | MS.gene45794:CDS | 30.0% | |
| AGCAATATACTCCTAACAAT+AGG | + | chr4.4:76887114-76887133 | None:intergenic | 30.0% | |
| GGATATCAATTAGTAAGAGA+TGG | - | chr4.4:76886769-76886788 | MS.gene45794:intron | 30.0% | |
| GTGAAACGATATCAAGATTA+TGG | - | chr4.4:76886265-76886284 | MS.gene45794:intron | 30.0% | |
| TCAATTAGTAAGAGATGGTA+CGG | - | chr4.4:76886774-76886793 | MS.gene45794:intron | 30.0% | |
| TTGCATTCATTGAATTTGTC+AGG | - | chr4.4:76887180-76887199 | MS.gene45794:intron | 30.0% | |
| ! | ATCCTTGCTAACATTTTCTA+AGG | + | chr4.4:76887239-76887258 | None:intergenic | 30.0% |
| ! | CTTCATTTTGTTCTTGCATT+TGG | - | chr4.4:76887345-76887364 | MS.gene45794:CDS | 30.0% |
| ! | TCCTTGCTAACATTTTCTAA+GGG | + | chr4.4:76887238-76887257 | None:intergenic | 30.0% |
| !! | CTACAAAAGTACCTATTGTT+AGG | - | chr4.4:76887100-76887119 | MS.gene45794:intron | 30.0% |
| !!! | TGTTATTTGGGTTTTTGAGT+TGG | - | chr4.4:76886730-76886749 | MS.gene45794:intron | 30.0% |
| AAACCTACATGTCATTGAAG+TGG | + | chr4.4:76887154-76887173 | None:intergenic | 35.0% | |
| AAATGTTAGCAAGGATGTGT+TGG | - | chr4.4:76887243-76887262 | MS.gene45794:intron | 35.0% | |
| AATGTTAGCAAGGATGTGTT+GGG | - | chr4.4:76887244-76887263 | MS.gene45794:intron | 35.0% | |
| AGATGGTACGGAATCAATTA+CGG | - | chr4.4:76886786-76886805 | MS.gene45794:intron | 35.0% | |
| GAAATGTGAGCGAATACTTA+GGG | - | chr4.4:76886384-76886403 | MS.gene45794:intron | 35.0% | |
| TATCCACTTCAATGACATGT+AGG | - | chr4.4:76887148-76887167 | MS.gene45794:intron | 35.0% | |
| TTTCTTACCAATCGACCTTT+GGG | + | chr4.4:76886200-76886219 | None:intergenic | 35.0% | |
| ! | CCTTGCTAACATTTTCTAAG+GGG | + | chr4.4:76887237-76887256 | None:intergenic | 35.0% |
| ! | TGTTAGAAGTGTATGGTTGT+AGG | - | chr4.4:76887057-76887076 | MS.gene45794:intron | 35.0% |
| ! | TGTTAGGAGTATATTGCTGT+AGG | - | chr4.4:76887116-76887135 | MS.gene45794:intron | 35.0% |
| ! | TTTTCTTACCAATCGACCTT+TGG | + | chr4.4:76886201-76886220 | None:intergenic | 35.0% |
| !!! | ATTTGGGTTTTTGAGTTGGT+TGG | - | chr4.4:76886734-76886753 | MS.gene45794:intron | 35.0% |
| AGAGAAATCGAACTCGTAAG+GGG | + | chr4.4:76886063-76886082 | None:intergenic | 40.0% | |
| AGAGAGAAATCGAACTCGTA+AGG | + | chr4.4:76886065-76886084 | None:intergenic | 40.0% | |
| AGGTCGATTGGTAAGAAAAG+CGG | - | chr4.4:76886202-76886221 | MS.gene45794:intron | 40.0% | |
| CCCCTTAGAAAATGTTAGCA+AGG | - | chr4.4:76887234-76887253 | MS.gene45794:intron | 40.0% | |
| GAGAGAAATCGAACTCGTAA+GGG | + | chr4.4:76886064-76886083 | None:intergenic | 40.0% | |
| GCAACGTTGACACTATAGTA+TGG | - | chr4.4:76886844-76886863 | MS.gene45794:intron | 40.0% | |
| GGAAATGTGAGCGAATACTT+AGG | - | chr4.4:76886383-76886402 | MS.gene45794:intron | 40.0% | |
| GGAGTATATTGCTGTAGGTT+CGG | - | chr4.4:76887121-76887140 | MS.gene45794:intron | 40.0% | |
| TGTATAAGCTTCGATCCCAA+AGG | - | chr4.4:76886182-76886201 | MS.gene45794:intron | 40.0% | |
| TGTGTTGGGTTCTACTATTG+AGG | - | chr4.4:76887258-76887277 | MS.gene45794:intron | 40.0% | |
| TGTTGGGTTCACTTATGTGT+TGG | - | chr4.4:76887448-76887467 | MS.gene45794:CDS | 40.0% | |
| AGAGAATCGGAAGGTGAAAC+AGG | + | chr4.4:76886094-76886113 | None:intergenic | 45.0% | |
| AGGCCTTCAATTGGATACGA+TGG | - | chr4.4:76886879-76886898 | MS.gene45794:intron | 45.0% | |
| AGTGTATGGTTGTAGGTTGC+TGG | - | chr4.4:76887064-76887083 | MS.gene45794:intron | 45.0% | |
| AGTTGGTTGGAAGTTCTGCA+AGG | - | chr4.4:76886747-76886766 | MS.gene45794:intron | 45.0% | |
| ATCGAACTCGTAAGGGGTTT+AGG | + | chr4.4:76886057-76886076 | None:intergenic | 45.0% | |
| ATTGGTAAGAAAAGCGGCTC+TGG | - | chr4.4:76886208-76886227 | MS.gene45794:intron | 45.0% | |
| GTGCCATCGTATCCAATTGA+AGG | + | chr4.4:76886885-76886904 | None:intergenic | 45.0% | |
| GTTGGTTGGAAGTTCTGCAA+GGG | - | chr4.4:76886748-76886767 | MS.gene45794:intron | 45.0% | |
| TAGTATGGTGACATGAGTGC+AGG | - | chr4.4:76886859-76886878 | MS.gene45794:intron | 45.0% | |
| TCACGTGAAAGAGAATCGGA+AGG | + | chr4.4:76886103-76886122 | None:intergenic | 45.0% | |
| TCGAACTCGTAAGGGGTTTA+GGG | + | chr4.4:76886056-76886075 | None:intergenic | 45.0% | |
| !! | GAATCGCAAGAGTATTACGC+TGG | - | chr4.4:76886301-76886320 | MS.gene45794:intron | 45.0% |
| ATTACGCTGGTGGTCAGAAG+AGG | - | chr4.4:76886314-76886333 | MS.gene45794:intron | 50.0% | |
| CATGAGTGCAGGCCTTCAAT+TGG | - | chr4.4:76886870-76886889 | MS.gene45794:intron | 50.0% | |
| CTTCGATCCCAAAGGTCGAT+TGG | - | chr4.4:76886190-76886209 | MS.gene45794:intron | 50.0% | |
| GCGAAAGAGGAGAGTCATCA+TGG | + | chr4.4:76886132-76886151 | None:intergenic | 50.0% | |
| GGTACGGAATCAATTACGGC+TGG | - | chr4.4:76886790-76886809 | MS.gene45794:intron | 50.0% | |
| TGTGACAGCGATTGCGAAAG+AGG | + | chr4.4:76886145-76886164 | None:intergenic | 50.0% | |
| ! | AGAAAAGCGGCTCTGGTAGT+CGG | - | chr4.4:76886215-76886234 | MS.gene45794:intron | 50.0% |
| !! | TCGCAAGAGTATTACGCTGG+TGG | - | chr4.4:76886304-76886323 | MS.gene45794:intron | 50.0% |
| GCTCTGGTAGTCGGCAAAGT+GGG | - | chr4.4:76886224-76886243 | MS.gene45794:intron | 55.0% | |
| GGCTGGCACCTCTAAGCCTT+GGG | - | chr4.4:76886807-76886826 | MS.gene45794:intron | 60.0% | |
| ! | GGCTCTGGTAGTCGGCAAAG+TGG | - | chr4.4:76886223-76886242 | MS.gene45794:intron | 60.0% |
| CGGCTGGCACCTCTAAGCCT+TGG | - | chr4.4:76886806-76886825 | MS.gene45794:intron | 65.0% | |
| GCACCTCTAAGCCTTGGGCG+CGG | - | chr4.4:76886812-76886831 | MS.gene45794:intron | 65.0% | |
| TCTAAGCCTTGGGCGCGGCG+CGG | - | chr4.4:76886817-76886836 | MS.gene45794:intron | 70.0% | |
| GCGCCGCGCCCAAGGCTTAG+AGG | + | chr4.4:76886818-76886837 | None:intergenic | 75.0% | |
| ! | GCTTTGCCGCGCCGCGCCCA+AGG | + | chr4.4:76886826-76886845 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 76886037 | 76887562 | 76886037 | ID=MS.gene45794 |
| chr4.4 | mRNA | 76886037 | 76887562 | 76886037 | ID=MS.gene45794.t1;Parent=MS.gene45794 |
| chr4.4 | exon | 76887264 | 76887562 | 76887264 | ID=MS.gene45794.t1.exon1;Parent=MS.gene45794.t1 |
| chr4.4 | CDS | 76887264 | 76887562 | 76887264 | ID=cds.MS.gene45794.t1;Parent=MS.gene45794.t1 |
| chr4.4 | exon | 76886514 | 76886580 | 76886514 | ID=MS.gene45794.t1.exon2;Parent=MS.gene45794.t1 |
| chr4.4 | CDS | 76886514 | 76886580 | 76886514 | ID=cds.MS.gene45794.t1;Parent=MS.gene45794.t1 |
| chr4.4 | exon | 76886320 | 76886397 | 76886320 | ID=MS.gene45794.t1.exon3;Parent=MS.gene45794.t1 |
| chr4.4 | CDS | 76886320 | 76886397 | 76886320 | ID=cds.MS.gene45794.t1;Parent=MS.gene45794.t1 |
| chr4.4 | exon | 76886037 | 76886051 | 76886037 | ID=MS.gene45794.t1.exon4;Parent=MS.gene45794.t1 |
| chr4.4 | CDS | 76886037 | 76886051 | 76886037 | ID=cds.MS.gene45794.t1;Parent=MS.gene45794.t1 |
| Gene Sequence |
| Protein sequence |
