AlfalfaGEDB | MS.gene46066

M. sativa cultivar XinJiangDaYe / MS.gene46066

KEGG: -
GO: -
PFAM: PF14368.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46066.t1	XP_003623161.2	95.7	117	5	0	1	117	1	117	1.50E-57	231.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46066.t1	G7L5L2	95.7	117	5	0	1	117	1	117	1.1e-57	231.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04932	MS.gene46066	0.818096	2.34E-52	-1.69E-46
MS.gene050170	MS.gene46066	-0.832816	7.62E-56	-1.69E-46
MS.gene050904	MS.gene46066	-0.820055	8.39E-53	-1.69E-46
MS.gene051552	MS.gene46066	0.82366	1.23E-53	-1.69E-46
MS.gene051651	MS.gene46066	0.80313	4.04E-49	-1.69E-46
MS.gene052211	MS.gene46066	0.801446	8.97E-49	-1.69E-46
MS.gene05240	MS.gene46066	0.80121	1.00E-48	-1.69E-46
MS.gene052606	MS.gene46066	0.833472	5.23E-56	-1.69E-46
MS.gene053097	MS.gene46066	0.821671	3.57E-53	-1.69E-46
MS.gene054146	MS.gene46066	0.807597	4.68E-50	-1.69E-46
MS.gene054884	MS.gene46066	0.818676	1.73E-52	-1.69E-46
MS.gene055158	MS.gene46066	0.923946	1.36E-89	-1.69E-46
MS.gene055159	MS.gene46066	0.9296	5.50E-93	-1.69E-46
MS.gene05589	MS.gene46066	-0.822472	2.33E-53	-1.69E-46
MS.gene056198	MS.gene46066	0.811535	6.67E-51	-1.69E-46
MS.gene056387	MS.gene46066	-0.829661	4.54E-55	-1.69E-46
MS.gene056688	MS.gene46066	0.825579	4.34E-54	-1.69E-46
MS.gene056694	MS.gene46066	0.805355	1.39E-49	-1.69E-46
MS.gene056697	MS.gene46066	0.820243	7.60E-53	-1.69E-46
MS.gene056856	MS.gene46066	0.818127	2.30E-52	-1.69E-46
MS.gene058334	MS.gene46066	0.803655	3.14E-49	-1.69E-46
MS.gene058382	MS.gene46066	0.800783	1.23E-48	-1.69E-46
MS.gene058418	MS.gene46066	0.830893	2.27E-55	-1.69E-46
MS.gene058603	MS.gene46066	0.808104	3.65E-50	-1.69E-46
MS.gene05926	MS.gene46066	0.817852	2.66E-52	-1.69E-46
MS.gene05960	MS.gene46066	0.81925	1.28E-52	-1.69E-46
MS.gene059955	MS.gene46066	0.81342	2.58E-51	-1.69E-46
MS.gene06000	MS.gene46066	-0.825571	4.36E-54	-1.69E-46
MS.gene060593	MS.gene46066	0.819869	9.26E-53	-1.69E-46
MS.gene061044	MS.gene46066	0.812289	4.57E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46066.t1	MTR_7g065160	95.726	117	5	0	1	117	1	117	7.73e-80	230
MS.gene46066.t1	MTR_7g065210	87.179	117	15	0	1	117	1	117	6.74e-73	213
MS.gene46066.t1	MTR_8g070040	45.455	121	61	3	1	117	1	120	3.87e-25	92.0
MS.gene46066.t1	MTR_8g070015	42.105	114	61	3	1	110	1	113	2.30e-19	77.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene46066.t1	AT5G05960	61.261	111	36	2	7	117	13	116	1.06e-42	136
MS.gene46066.t1	AT3G53980	64.211	95	33	1	23	117	21	114	2.86e-42	135
MS.gene46066.t1	AT3G53980	64.211	95	33	1	23	117	21	114	2.86e-42	135
MS.gene46066.t1	AT2G37870	49.107	112	51	4	10	117	6	115	5.31e-26	94.0

Find 21 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTATTGCAGTTCTTGTTGC+AGG	0.361788	7.2:-42679984	MS.gene46066:CDS
AGCTTAAAAGCTTCATTGTC+TGG	0.411064	7.2:+42679915	None:intergenic
TCAAACGTTGCTAAAATGTC+TGG	0.431901	7.2:-42679780	MS.gene46066:CDS
CAGCAAGGTTGCAACGCTTA+GGG	0.465106	7.2:+42679730	None:intergenic
GGATAGTAACAGCAATTTGA+GGG	0.472152	7.2:+42679751	None:intergenic
TCAGCAAGGTTGCAACGCTT+AGG	0.488806	7.2:+42679729	None:intergenic
TGTGCTCAGGTTAAGAAACT+TGG	0.492815	7.2:-42679837	MS.gene46066:CDS
GTGAAATTGTTGTGCTTCGT+TGG	0.500117	7.2:-42680005	MS.gene46066:CDS
GGGATAGTAACAGCAATTTG+AGG	0.508534	7.2:+42679750	None:intergenic
GTCACAAAATTGTTGTGCTC+AGG	0.514410	7.2:-42679850	MS.gene46066:CDS
CGTCCACACTTGTAACCGAC+AGG	0.520932	7.2:+42679702	None:intergenic
CGCCCTGTCGGTTACAAGTG+TGG	0.534054	7.2:-42679705	MS.gene46066:CDS
GTTGAAAGTGCTGGTGAATG+TGG	0.539242	7.2:-42679951	MS.gene46066:CDS
AAAGTGCTGGTGAATGTGGA+AGG	0.549386	7.2:-42679947	MS.gene46066:CDS
AACCTTGCTGATCGCCCTGT+CGG	0.551684	7.2:-42679717	MS.gene46066:CDS
ATTCATAGAGTTGAAAGTGC+TGG	0.554882	7.2:-42679960	MS.gene46066:CDS
ATAACAGCACAAAGACAACT+TGG	0.604458	7.2:+42679807	None:intergenic
AGTGCTGGTGAATGTGGAAG+GGG	0.630405	7.2:-42679945	MS.gene46066:CDS
AACCGACAGGGCGATCAGCA+AGG	0.631783	7.2:+42679715	None:intergenic
AAGTGCTGGTGAATGTGGAA+GGG	0.641454	7.2:-42679946	MS.gene46066:CDS
GTCCACACTTGTAACCGACA+GGG	0.709589	7.2:+42679703	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATTATTTATCTTTTTTCT+TGG	-	chr7.2:42679952-42679971	MS.gene46066:CDS	10.0%
!!!	ACTTATTTGACATTTTTTTT+AGG	+	chr7.2:42679913-42679932	None:intergenic	15.0%
	AGCTTAAAAGCTTCATTGTC+TGG	+	chr7.2:42679463-42679482	None:intergenic	35.0%
	ATAACAGCACAAAGACAACT+TGG	+	chr7.2:42679571-42679590	None:intergenic	35.0%
	GGATAGTAACAGCAATTTGA+GGG	+	chr7.2:42679627-42679646	None:intergenic	35.0%
	TCAAACGTTGCTAAAATGTC+TGG	-	chr7.2:42679595-42679614	MS.gene46066:intron	35.0%
!	ATTCATAGAGTTGAAAGTGC+TGG	-	chr7.2:42679415-42679434	MS.gene46066:intron	35.0%
	GGGATAGTAACAGCAATTTG+AGG	+	chr7.2:42679628-42679647	None:intergenic	40.0%
	GTCACAAAATTGTTGTGCTC+AGG	-	chr7.2:42679525-42679544	MS.gene46066:intron	40.0%
	GTGAAATTGTTGTGCTTCGT+TGG	-	chr7.2:42679370-42679389	MS.gene46066:intron	40.0%
	TGTGCTCAGGTTAAGAAACT+TGG	-	chr7.2:42679538-42679557	MS.gene46066:intron	40.0%
	GGTATTGCAGTTCTTGTTGC+AGG	-	chr7.2:42679391-42679410	MS.gene46066:intron	45.0%
!	TCTTTTGCTGCACTTGCACA+AGG	+	chr7.2:42679490-42679509	None:intergenic	45.0%
!!	AAAGTGCTGGTGAATGTGGA+AGG	-	chr7.2:42679428-42679447	MS.gene46066:intron	45.0%
!!	AAGTGCTGGTGAATGTGGAA+GGG	-	chr7.2:42679429-42679448	MS.gene46066:intron	45.0%
!!	GTTGAAAGTGCTGGTGAATG+TGG	-	chr7.2:42679424-42679443	MS.gene46066:intron	45.0%
	CAGCAAGGTTGCAACGCTTA+GGG	+	chr7.2:42679648-42679667	None:intergenic	50.0%
	GTCCACACTTGTAACCGACA+GGG	+	chr7.2:42679675-42679694	None:intergenic	50.0%
	TCAGCAAGGTTGCAACGCTT+AGG	+	chr7.2:42679649-42679668	None:intergenic	50.0%
!!	AGTGCTGGTGAATGTGGAAG+GGG	-	chr7.2:42679430-42679449	MS.gene46066:intron	50.0%
	AACCTTGCTGATCGCCCTGT+CGG	-	chr7.2:42679658-42679677	MS.gene46066:intron	55.0%
	CGTCCACACTTGTAACCGAC+AGG	+	chr7.2:42679676-42679695	None:intergenic	55.0%
	AACCGACAGGGCGATCAGCA+AGG	+	chr7.2:42679663-42679682	None:intergenic	60.0%
!	CGCCCTGTCGGTTACAAGTG+TGG	-	chr7.2:42679670-42679689	MS.gene46066:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	42679358	42680039	42679358	ID=MS.gene46066
chr7.2	mRNA	42679358	42680039	42679358	ID=MS.gene46066.t1;Parent=MS.gene46066
chr7.2	exon	42679703	42680039	42679703	ID=MS.gene46066.t1.exon1;Parent=MS.gene46066.t1
chr7.2	CDS	42679703	42680039	42679703	ID=cds.MS.gene46066.t1;Parent=MS.gene46066.t1
chr7.2	exon	42679358	42679374	42679358	ID=MS.gene46066.t1.exon2;Parent=MS.gene46066.t1
chr7.2	CDS	42679358	42679374	42679358	ID=cds.MS.gene46066.t1;Parent=MS.gene46066.t1

Gene Sequence

>MS.gene46066

ATGGAGGGTCTTGTGAAATTGTTGTGCTTCGTTGGTATTGCAGTTCTTGTTGCAGGAATTCATAGAGTTGAAAGTGCTGGTGAATGTGGAAGGGGTACTACACCAGACAATGAAGCTTTTAAGCTACTTCCTTGTGCAAGTGCAGCAAAAGATGAAAATGCTAGTGTGTCACAAAATTGTTGTGCTCAGGTTAAGAAACTTGGTCAGAATCCAAGTTGTCTTTGTGCTGTTATGCTTTCAAACGTTGCTAAAATGTCTGGTGCTAACCCTCAAATTGCTGTTACTATCCCTAAGCGTTGCAACCTTGCTGATCGCCCTGTCGGTTACAAGTGTGGACGTAAGTTACTAACTACTCTCTGTCGTTTTTGTAAAAAAATTGTTCGTATTTATTTGTTGTTTTCAAAGCTCAATGTCAGAGAAATAAGCGAAATAAAACTATAAATAGTTAAGAGTATTATAGAAAATAGATAAATAATTCTATCAAAAGTAGAAAAAAAATAAAAAAATTGCTAAAATCTACTTTCTTTTTATAGAACTAAAAACATATTTATGCCTAAAAAAAATGTCAAATAAGTATGCTGCTAATATATGTTTTAATTATTTATCTTTTTTCTTGGTAGATCAATATGCTAGCTAATTCTTTTCACAATCTCTTAATATTGCAGCTTACACTCTTCCTTGA

Protein sequence

>MS.gene46066.t1

MEGLVKLLCFVGIAVLVAGIHRVESAGECGRGTTPDNEAFKLLPCASAAKDENASVSQNCCAQVKKLGQNPSCLCAVMLSNVAKMSGANPQIAVTIPKRCNLADRPVGYKCGPYTLP