Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47276.t1 | KEH25543.1 | 84.2 | 95 | 8 | 1 | 1 | 95 | 1 | 88 | 8.80E-35 | 156 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47276.t1 | A0A072U8W6 | 84.2 | 95 | 8 | 1 | 1 | 95 | 1 | 88 | 6.3e-35 | 156.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene47276 | 0.804698 | 1.91E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene47276 | 0.805934 | 1.05E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene47276 | 0.806423 | 8.29E-50 | -1.69E-46 |
| MS.gene049499 | MS.gene47276 | 0.844353 | 7.97E-59 | -1.69E-46 |
| MS.gene049711 | MS.gene47276 | 0.806612 | 7.56E-50 | -1.69E-46 |
| MS.gene051019 | MS.gene47276 | 0.846202 | 2.52E-59 | -1.69E-46 |
| MS.gene051699 | MS.gene47276 | 0.840411 | 8.84E-58 | -1.69E-46 |
| MS.gene052533 | MS.gene47276 | 0.837276 | 5.72E-57 | -1.69E-46 |
| MS.gene052793 | MS.gene47276 | 0.860282 | 2.30E-63 | -1.69E-46 |
| MS.gene053228 | MS.gene47276 | 0.812677 | 3.76E-51 | -1.69E-46 |
| MS.gene053272 | MS.gene47276 | -0.855154 | 7.63E-62 | -1.69E-46 |
| MS.gene054142 | MS.gene47276 | 0.802455 | 5.56E-49 | -1.69E-46 |
| MS.gene055150 | MS.gene47276 | 0.843021 | 1.81E-58 | -1.69E-46 |
| MS.gene055724 | MS.gene47276 | 0.807784 | 4.27E-50 | -1.69E-46 |
| MS.gene05624 | MS.gene47276 | 0.810234 | 1.28E-50 | -1.69E-46 |
| MS.gene056677 | MS.gene47276 | 0.818916 | 1.53E-52 | -1.69E-46 |
| MS.gene056699 | MS.gene47276 | 0.824401 | 8.23E-54 | -1.69E-46 |
| MS.gene05686 | MS.gene47276 | 0.802503 | 5.44E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene47276 | 0.855261 | 7.11E-62 | -1.69E-46 |
| MS.gene057132 | MS.gene47276 | 0.801275 | 9.72E-49 | -1.69E-46 |
| MS.gene058159 | MS.gene47276 | 0.80984 | 1.55E-50 | -1.69E-46 |
| MS.gene058592 | MS.gene47276 | 0.824978 | 6.02E-54 | -1.69E-46 |
| MS.gene058781 | MS.gene47276 | 0.814987 | 1.16E-51 | -1.69E-46 |
| MS.gene05952 | MS.gene47276 | 0.845319 | 4.37E-59 | -1.69E-46 |
| MS.gene05985 | MS.gene47276 | -0.847113 | 1.42E-59 | -1.69E-46 |
| MS.gene060244 | MS.gene47276 | 0.805992 | 1.02E-49 | -1.69E-46 |
| MS.gene060338 | MS.gene47276 | 0.815249 | 1.02E-51 | -1.69E-46 |
| MS.gene060512 | MS.gene47276 | 0.800461 | 1.43E-48 | -1.69E-46 |
| MS.gene060935 | MS.gene47276 | 0.836415 | 9.49E-57 | -1.69E-46 |
| MS.gene060937 | MS.gene47276 | 0.847791 | 9.25E-60 | -1.69E-46 |
| MS.gene061100 | MS.gene47276 | 0.817295 | 3.55E-52 | -1.69E-46 |
| MS.gene061188 | MS.gene47276 | 0.867804 | 1.04E-65 | -1.69E-46 |
| MS.gene061243 | MS.gene47276 | 0.874644 | 5.75E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47276.t1 | MTR_6g027480 | 84.211 | 95 | 8 | 1 | 1 | 95 | 1 | 88 | 2.27e-53 | 161 |
| MS.gene47276.t1 | MTR_7g086770 | 50.649 | 77 | 33 | 2 | 17 | 92 | 20 | 92 | 1.35e-15 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACCACTCCCAGGGGATTT+TGG | 0.070443 | 6.3:-19023821 | None:intergenic |
| GATGGGAAGAAGGGTCAATT+TGG | 0.296094 | 6.3:+19023754 | MS.gene47276:CDS |
| GGTTCATTGATGTTGGAAAA+AGG | 0.327022 | 6.3:+19023698 | MS.gene47276:CDS |
| CACCAGGTCCTTCATAATCA+TGG | 0.394345 | 6.3:-19023781 | None:intergenic |
| ATGAAAGATTGATGGGAAGA+AGG | 0.472148 | 6.3:+19023744 | MS.gene47276:CDS |
| TGAGAAAATGATGGTACATA+TGG | 0.477957 | 6.3:+19023721 | MS.gene47276:CDS |
| TGAAAGATTGATGGGAAGAA+GGG | 0.490161 | 6.3:+19023745 | MS.gene47276:CDS |
| ACATATGGATGAAAGATTGA+TGG | 0.490804 | 6.3:+19023736 | MS.gene47276:CDS |
| TTGGAACTCCATGATTATGA+AGG | 0.505871 | 6.3:+19023773 | MS.gene47276:CDS |
| GCACTAACCTTATATAAAGA+AGG | 0.507798 | 6.3:-19022989 | None:intergenic |
| TTATGTTCTTTATTTGCACC+AGG | 0.512006 | 6.3:-19023797 | None:intergenic |
| GGAACATGGTTCATTGATGT+TGG | 0.518756 | 6.3:+19023691 | MS.gene47276:intron |
| CATATGGATGAAAGATTGAT+GGG | 0.518975 | 6.3:+19023737 | MS.gene47276:CDS |
| GAACATAACCCAAAATCCCC+TGG | 0.526511 | 6.3:+19023812 | MS.gene47276:CDS |
| AACCCAAAATCCCCTGGGAG+TGG | 0.548305 | 6.3:+19023818 | MS.gene47276:CDS |
| TTTGCACATCAACCACTCCC+AGG | 0.556615 | 6.3:-19023830 | None:intergenic |
| CTCCATGATTATGAAGGACC+TGG | 0.576982 | 6.3:+19023779 | MS.gene47276:CDS |
| TTGCACATCAACCACTCCCA+GGG | 0.587694 | 6.3:-19023829 | None:intergenic |
| AACATAACCCAAAATCCCCT+GGG | 0.609618 | 6.3:+19023813 | MS.gene47276:CDS |
| TTGCAGGGATAGCAAAGACA+TGG | 0.624053 | 6.3:-19022839 | None:intergenic |
| TGCACATCAACCACTCCCAG+GGG | 0.679834 | 6.3:-19023828 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGTATTGAATTGATTTTTT+TGG | + | chr6.3:19022922-19022941 | MS.gene47276:intron | 15.0% |
| !!! | GTTAATTACTTAAACTTTTT+TGG | - | chr6.3:19023626-19023645 | None:intergenic | 15.0% |
| !!! | TTTACTTTTCAAATTTTTGT+TGG | + | chr6.3:19023265-19023284 | MS.gene47276:intron | 15.0% |
| !!! | TTTTTTATTCTTTTAAATCG+TGG | - | chr6.3:19023399-19023418 | None:intergenic | 15.0% |
| !!! | AACTTTTTTGGCAAAAATTA+AGG | - | chr6.3:19023614-19023633 | None:intergenic | 20.0% |
| !!! | AGTTTTTCCTTCTTTATATA+AGG | + | chr6.3:19022982-19023001 | MS.gene47276:CDS | 20.0% |
| !!! | ATTTTTTCATCCAATATCTA+AGG | + | chr6.3:19023076-19023095 | MS.gene47276:intron | 20.0% |
| !!! | CTTCTTAATTTTCATCTATT+TGG | + | chr6.3:19023011-19023030 | MS.gene47276:intron | 20.0% |
| !! | AAAGTCATGGTTATGTATTT+TGG | + | chr6.3:19023051-19023070 | MS.gene47276:intron | 25.0% |
| !! | ATCTTCACCATTCAAATTTT+TGG | - | chr6.3:19022957-19022976 | None:intergenic | 25.0% |
| !! | TCATTATTTGTGTGTTTTGT+AGG | + | chr6.3:19023670-19023689 | MS.gene47276:intron | 25.0% |
| !!! | GAATTGATTTTTTTGGTTGT+AGG | + | chr6.3:19022929-19022948 | MS.gene47276:intron | 25.0% |
| ACATATGGATGAAAGATTGA+TGG | + | chr6.3:19023736-19023755 | MS.gene47276:CDS | 30.0% | |
| CATATGGATGAAAGATTGAT+GGG | + | chr6.3:19023737-19023756 | MS.gene47276:CDS | 30.0% | |
| CCAATATCTAAGGAAAAATG+AGG | + | chr6.3:19023086-19023105 | MS.gene47276:intron | 30.0% | |
| GCACTAACCTTATATAAAGA+AGG | - | chr6.3:19022992-19023011 | None:intergenic | 30.0% | |
| GTAGGAACCAAAAATTTGAA+TGG | + | chr6.3:19022947-19022966 | MS.gene47276:intron | 30.0% | |
| TGAGAAAATGATGGTACATA+TGG | + | chr6.3:19023721-19023740 | MS.gene47276:CDS | 30.0% | |
| ! | TTATGTTCTTTATTTGCACC+AGG | - | chr6.3:19023800-19023819 | None:intergenic | 30.0% |
| !! | CCTCATTTTTCCTTAGATAT+TGG | - | chr6.3:19023089-19023108 | None:intergenic | 30.0% |
| AACCATGACTTTGCAAGAAT+AGG | - | chr6.3:19023043-19023062 | None:intergenic | 35.0% | |
| ATGAAAGATTGATGGGAAGA+AGG | + | chr6.3:19023744-19023763 | MS.gene47276:CDS | 35.0% | |
| GGAAAAAGGTGAGAAAATGA+TGG | + | chr6.3:19023712-19023731 | MS.gene47276:CDS | 35.0% | |
| GGTTCATTGATGTTGGAAAA+AGG | + | chr6.3:19023698-19023717 | MS.gene47276:CDS | 35.0% | |
| TGAAAGATTGATGGGAAGAA+GGG | + | chr6.3:19023745-19023764 | MS.gene47276:CDS | 35.0% | |
| TTCCTATTCTTGCAAAGTCA+TGG | + | chr6.3:19023038-19023057 | MS.gene47276:intron | 35.0% | |
| TTGGAACTCCATGATTATGA+AGG | + | chr6.3:19023773-19023792 | MS.gene47276:CDS | 35.0% | |
| ! | CAAAAACCTTACTTGTTGCA+GGG | - | chr6.3:19022857-19022876 | None:intergenic | 35.0% |
| ! | TCAAAAACCTTACTTGTTGC+AGG | - | chr6.3:19022858-19022877 | None:intergenic | 35.0% |
| ! | TTGTGTGTTTTGTAGGAACA+TGG | + | chr6.3:19023677-19023696 | MS.gene47276:intron | 35.0% |
| AACATAACCCAAAATCCCCT+GGG | + | chr6.3:19023813-19023832 | MS.gene47276:CDS | 40.0% | |
| CAACAATTGTGTCAAGCAGA+AGG | + | chr6.3:19023219-19023238 | MS.gene47276:intron | 40.0% | |
| GGAACATGGTTCATTGATGT+TGG | + | chr6.3:19023691-19023710 | MS.gene47276:intron | 40.0% | |
| CACCAGGTCCTTCATAATCA+TGG | - | chr6.3:19023784-19023803 | None:intergenic | 45.0% | |
| CTCCATGATTATGAAGGACC+TGG | + | chr6.3:19023779-19023798 | MS.gene47276:CDS | 45.0% | |
| GAACATAACCCAAAATCCCC+TGG | + | chr6.3:19023812-19023831 | MS.gene47276:CDS | 45.0% | |
| GATGGGAAGAAGGGTCAATT+TGG | + | chr6.3:19023754-19023773 | MS.gene47276:CDS | 45.0% | |
| TGCTATCCCTGCAACAAGTA+AGG | + | chr6.3:19022848-19022867 | MS.gene47276:intron | 45.0% | |
| TTGCAGGGATAGCAAAGACA+TGG | - | chr6.3:19022842-19022861 | None:intergenic | 45.0% | |
| !!! | ATTAACTTTTTAATTTATTT+TGG | + | chr6.3:19023640-19023659 | MS.gene47276:intron | 5.0% |
| ! | AACCACTCCCAGGGGATTTT+GGG | - | chr6.3:19023823-19023842 | None:intergenic | 50.0% |
| AACCCAAAATCCCCTGGGAG+TGG | + | chr6.3:19023818-19023837 | MS.gene47276:CDS | 55.0% | |
| ! | CAACCACTCCCAGGGGATTT+TGG | - | chr6.3:19023824-19023843 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 19022783 | 19023844 | 19022783 | ID=MS.gene47276 |
| chr6.3 | mRNA | 19022783 | 19023844 | 19022783 | ID=MS.gene47276.t1;Parent=MS.gene47276 |
| chr6.3 | exon | 19022783 | 19022864 | 19022783 | ID=MS.gene47276.t1.exon1;Parent=MS.gene47276.t1 |
| chr6.3 | CDS | 19022783 | 19022864 | 19022783 | ID=cds.MS.gene47276.t1;Parent=MS.gene47276.t1 |
| chr6.3 | exon | 19022951 | 19023003 | 19022951 | ID=MS.gene47276.t1.exon2;Parent=MS.gene47276.t1 |
| chr6.3 | CDS | 19022951 | 19023003 | 19022951 | ID=cds.MS.gene47276.t1;Parent=MS.gene47276.t1 |
| chr6.3 | exon | 19023692 | 19023844 | 19023692 | ID=MS.gene47276.t1.exon3;Parent=MS.gene47276.t1 |
| chr6.3 | CDS | 19023692 | 19023844 | 19023692 | ID=cds.MS.gene47276.t1;Parent=MS.gene47276.t1 |
| Gene Sequence |
| Protein sequence |
