Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48393.t1 | XP_003608942.1 | 96.6 | 178 | 6 | 0 | 1 | 178 | 1 | 178 | 4.50E-85 | 323.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48393.t1 | Q9FYL8 | 58.3 | 144 | 60 | 0 | 30 | 173 | 42 | 185 | 8.5e-40 | 164.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48393.t1 | G7JNT6 | 96.6 | 178 | 6 | 0 | 1 | 178 | 1 | 178 | 3.3e-85 | 323.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene48393 | -0.862801 | 3.92E-64 | -1.69E-46 |
| MS.gene049166 | MS.gene48393 | -0.801564 | 8.48E-49 | -1.69E-46 |
| MS.gene04917 | MS.gene48393 | -0.854117 | 1.52E-61 | -1.69E-46 |
| MS.gene049822 | MS.gene48393 | -0.819474 | 1.14E-52 | -1.69E-46 |
| MS.gene04984 | MS.gene48393 | -0.809862 | 1.53E-50 | -1.69E-46 |
| MS.gene04991 | MS.gene48393 | 0.84141 | 4.83E-58 | -1.69E-46 |
| MS.gene050114 | MS.gene48393 | -0.860677 | 1.75E-63 | -1.69E-46 |
| MS.gene050317 | MS.gene48393 | -0.826535 | 2.57E-54 | -1.69E-46 |
| MS.gene050623 | MS.gene48393 | 0.816574 | 5.15E-52 | -1.69E-46 |
| MS.gene051184 | MS.gene48393 | -0.820045 | 8.44E-53 | -1.69E-46 |
| MS.gene051245 | MS.gene48393 | -0.819374 | 1.20E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene48393 | -0.80778 | 4.28E-50 | -1.69E-46 |
| MS.gene051987 | MS.gene48393 | -0.807649 | 4.56E-50 | -1.69E-46 |
| MS.gene052242 | MS.gene48393 | 0.80213 | 6.49E-49 | -1.69E-46 |
| MS.gene052302 | MS.gene48393 | -0.804556 | 2.04E-49 | -1.69E-46 |
| MS.gene05344 | MS.gene48393 | 0.843765 | 1.15E-58 | -1.69E-46 |
| MS.gene055441 | MS.gene48393 | -0.81391 | 2.02E-51 | -1.69E-46 |
| MS.gene056097 | MS.gene48393 | -0.811405 | 7.12E-51 | -1.69E-46 |
| MS.gene056464 | MS.gene48393 | 0.825665 | 4.14E-54 | -1.69E-46 |
| MS.gene056752 | MS.gene48393 | -0.816887 | 4.39E-52 | -1.69E-46 |
| MS.gene056787 | MS.gene48393 | -0.808446 | 3.08E-50 | -1.69E-46 |
| MS.gene056830 | MS.gene48393 | 0.823665 | 1.23E-53 | -1.69E-46 |
| MS.gene056849 | MS.gene48393 | -0.871354 | 7.28E-67 | -1.69E-46 |
| MS.gene056975 | MS.gene48393 | -0.868148 | 8.10E-66 | -1.69E-46 |
| MS.gene057451 | MS.gene48393 | 0.847642 | 1.02E-59 | -1.69E-46 |
| MS.gene057452 | MS.gene48393 | 0.867176 | 1.66E-65 | -1.69E-46 |
| MS.gene057999 | MS.gene48393 | -0.821536 | 3.83E-53 | -1.69E-46 |
| MS.gene058238 | MS.gene48393 | -0.823071 | 1.69E-53 | -1.69E-46 |
| MS.gene058661 | MS.gene48393 | 0.811266 | 7.63E-51 | -1.69E-46 |
| MS.gene059050 | MS.gene48393 | -0.82292 | 1.83E-53 | -1.69E-46 |
| MS.gene059872 | MS.gene48393 | 0.864037 | 1.62E-64 | -1.69E-46 |
| MS.gene060084 | MS.gene48393 | -0.84248 | 2.52E-58 | -1.69E-46 |
| MS.gene06010 | MS.gene48393 | -0.804522 | 2.08E-49 | -1.69E-46 |
| MS.gene06011 | MS.gene48393 | -0.802824 | 4.67E-49 | -1.69E-46 |
| MS.gene060297 | MS.gene48393 | -0.847617 | 1.03E-59 | -1.69E-46 |
| MS.gene060563 | MS.gene48393 | -0.805082 | 1.58E-49 | -1.69E-46 |
| MS.gene060869 | MS.gene48393 | -0.811253 | 7.68E-51 | -1.69E-46 |
| MS.gene060870 | MS.gene48393 | -0.800186 | 1.62E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48393.t1 | MTR_4g106640 | 96.629 | 178 | 6 | 0 | 1 | 178 | 1 | 178 | 4.44e-124 | 347 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48393.t1 | AT1G24450 | 58.333 | 144 | 60 | 0 | 30 | 173 | 42 | 185 | 1.06e-56 | 177 |
Find 46 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGCAAACCCTAGTTGTTTC+TGG | 0.221287 | 8.3:+17028188 | None:intergenic |
| ACTGTTCATGGTCGTGATCT+TGG | 0.284490 | 8.3:-17027686 | MS.gene48393:CDS |
| GGTGCTATTTCTCTTGATAC+TGG | 0.322054 | 8.3:-17027740 | MS.gene48393:CDS |
| CTGTTCATGGTCGTGATCTT+GGG | 0.329301 | 8.3:-17027685 | MS.gene48393:CDS |
| CAAGCGACGGTTCAACTCTT+TGG | 0.330495 | 8.3:+17027898 | None:intergenic |
| TGCCGTTGATGCAATGCGTT+TGG | 0.351005 | 8.3:-17027846 | MS.gene48393:CDS |
| TCCCCATGCTACCATCGTTC+AGG | 0.352766 | 8.3:-17028339 | MS.gene48393:intron |
| ATGAGTCGACATTGGAGATT+AGG | 0.358387 | 8.3:+17027873 | None:intergenic |
| TTCTCTTGATACTGGTAGTT+CGG | 0.366585 | 8.3:-17027732 | MS.gene48393:CDS |
| TCTTCCACTGCTGTGGTTTG+TGG | 0.372837 | 8.3:-17027782 | MS.gene48393:CDS |
| GTTTCGAGGGCGGATGAAAA+TGG | 0.383610 | 8.3:+17028214 | None:intergenic |
| CACAGCAGTGGAAGAAGAAT+TGG | 0.384003 | 8.3:+17027790 | None:intergenic |
| GAAAGCTTTGTTGTTCTCTT+CGG | 0.417815 | 8.3:+17027994 | None:intergenic |
| TGAGTCGACATTGGAGATTA+GGG | 0.444608 | 8.3:+17027874 | None:intergenic |
| CAATTCTTCTTCCACTGCTG+TGG | 0.459397 | 8.3:-17027789 | MS.gene48393:CDS |
| GCCGTTGATGCAATGCGTTT+GGG | 0.462426 | 8.3:-17027845 | MS.gene48393:CDS |
| GTTGTTTCTGGAGGGTTTCG+AGG | 0.471552 | 8.3:+17028200 | None:intergenic |
| ACCCAAACGCATTGCATCAA+CGG | 0.482026 | 8.3:+17027844 | None:intergenic |
| AGTGGAAGAAGAATTGGTCT+TGG | 0.487457 | 8.3:+17027796 | None:intergenic |
| GTGGAAGAAGAATTGGTCTT+GGG | 0.501015 | 8.3:+17027797 | None:intergenic |
| CAAACCCTAGTTGTTTCTGG+AGG | 0.510081 | 8.3:+17028191 | None:intergenic |
| TTGTTTCTGGAGGGTTTCGA+GGG | 0.515270 | 8.3:+17028201 | None:intergenic |
| GAAACCCTCCAGAAACAACT+AGG | 0.522143 | 8.3:-17028196 | MS.gene48393:CDS |
| TACTGGTAGTTCGGAGTCTG+CGG | 0.528726 | 8.3:-17027723 | MS.gene48393:CDS |
| AACCTGAACGATGGTAGCAT+GGG | 0.530303 | 8.3:+17028337 | None:intergenic |
| AAACCCTAGTTGTTTCTGGA+GGG | 0.539563 | 8.3:+17028192 | None:intergenic |
| AAACCTGAACGATGGTAGCA+TGG | 0.544111 | 8.3:+17028336 | None:intergenic |
| CTCTTCGGAGAAAGAAGCGT+GGG | 0.560654 | 8.3:+17028009 | None:intergenic |
| TTTCTGGAGGGTTTCGAGGG+CGG | 0.561747 | 8.3:+17028204 | None:intergenic |
| GCGACGGTTCAACTCTTTGG+CGG | 0.570405 | 8.3:+17027901 | None:intergenic |
| AAGGATCGCAATGAGGAAGA+TGG | 0.572481 | 8.3:+17028361 | None:intergenic |
| ACTGGTAGTTCGGAGTCTGC+GGG | 0.579104 | 8.3:-17027722 | MS.gene48393:CDS |
| TGTTCATGGTCGTGATCTTG+GGG | 0.587592 | 8.3:-17027684 | MS.gene48393:CDS |
| GGCACAAGATGAGTCGACAT+TGG | 0.593448 | 8.3:+17027865 | None:intergenic |
| AGCACCACAAACCACAGCAG+TGG | 0.604051 | 8.3:+17027778 | None:intergenic |
| GAAGATGGTGAAGACGATGA+AGG | 0.605415 | 8.3:+17028376 | None:intergenic |
| GAACGATGGTAGCATGGGGA+AGG | 0.610380 | 8.3:+17028342 | None:intergenic |
| GCGTTTGGGTTTGCACAAGG+TGG | 0.620407 | 8.3:-17027831 | MS.gene48393:CDS |
| AATGCGTTTGGGTTTGCACA+AGG | 0.636231 | 8.3:-17027834 | MS.gene48393:CDS |
| AAACCCTCCAGAAACAACTA+GGG | 0.642154 | 8.3:-17028195 | MS.gene48393:intron |
| CATGGGGAAGGATCGCAATG+AGG | 0.653551 | 8.3:+17028354 | None:intergenic |
| GAAGACGATGAAGGTACGAG+TGG | 0.655609 | 8.3:+17028385 | None:intergenic |
| TGGAGATTAGGGACAAGCGA+CGG | 0.678870 | 8.3:+17027885 | None:intergenic |
| ACCTGAACGATGGTAGCATG+GGG | 0.683989 | 8.3:+17028338 | None:intergenic |
| TCTCTTCGGAGAAAGAAGCG+TGG | 0.707050 | 8.3:+17028008 | None:intergenic |
| GGAAAAGGAAACCTGAACGA+TGG | 0.708272 | 8.3:+17028328 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAACAAATTAAATAGAATT+TGG | + | chr8.3:17027963-17027982 | None:intergenic | 10.0% |
| !! | ATAGAATAAAATGAAAAGAG+AGG | + | chr8.3:17027917-17027936 | None:intergenic | 20.0% |
| !! | TAGAATAAAATGAAAAGAGA+GGG | + | chr8.3:17027916-17027935 | None:intergenic | 20.0% |
| !!! | ATGAAAACTGTTTCTATTAA+TGG | + | chr8.3:17027793-17027812 | None:intergenic | 20.0% |
| !!! | TTAATGGATTGTTTCGATTT+TGG | + | chr8.3:17027777-17027796 | None:intergenic | 25.0% |
| ATGTGTATTACATGATGATC+AGG | - | chr8.3:17027987-17028006 | MS.gene48393:CDS | 30.0% | |
| GAGAAAAATAAGAGAGAAAG+GGG | + | chr8.3:17027755-17027774 | None:intergenic | 30.0% | |
| GGAGAAAAATAAGAGAGAAA+GGG | + | chr8.3:17027756-17027775 | None:intergenic | 30.0% | |
| TCATTAATCTTGTACTTGCA+GGG | - | chr8.3:17027809-17027828 | MS.gene48393:CDS | 30.0% | |
| TGGAGAAAAATAAGAGAGAA+AGG | + | chr8.3:17027757-17027776 | None:intergenic | 30.0% | |
| TTCATTAATCTTGTACTTGC+AGG | - | chr8.3:17027808-17027827 | MS.gene48393:CDS | 30.0% | |
| ! | AACAAAGCTTTCAGCATTTT+AGG | - | chr8.3:17028076-17028095 | MS.gene48393:intron | 30.0% |
| ! | AATGTTTTCTTGACTGTTCA+TGG | - | chr8.3:17028361-17028380 | MS.gene48393:CDS | 30.0% |
| AATAAGAGAGAAAGGGGAAA+AGG | + | chr8.3:17027749-17027768 | None:intergenic | 35.0% | |
| AGAGAGGGTTTCTGAATAAA+AGG | + | chr8.3:17027901-17027920 | None:intergenic | 35.0% | |
| GGTTTCTGAATAAAAGGACA+GGG | + | chr8.3:17027895-17027914 | None:intergenic | 35.0% | |
| GTTTCTGAATAAAAGGACAG+GGG | + | chr8.3:17027894-17027913 | None:intergenic | 35.0% | |
| TTCTCTTGATACTGGTAGTT+CGG | - | chr8.3:17028327-17028346 | MS.gene48393:intron | 35.0% | |
| ! | GGTGCTTTTCGATCAATTTT+TGG | - | chr8.3:17028298-17028317 | MS.gene48393:intron | 35.0% |
| !! | GAAAGCTTTGTTGTTCTCTT+CGG | + | chr8.3:17028068-17028087 | None:intergenic | 35.0% |
| AAACCCTCCAGAAACAACTA+GGG | - | chr8.3:17027864-17027883 | MS.gene48393:CDS | 40.0% | |
| ATGAGTCGACATTGGAGATT+AGG | + | chr8.3:17028189-17028208 | None:intergenic | 40.0% | |
| GGGTTTCTGAATAAAAGGAC+AGG | + | chr8.3:17027896-17027915 | None:intergenic | 40.0% | |
| TGAGTCGACATTGGAGATTA+GGG | + | chr8.3:17028188-17028207 | None:intergenic | 40.0% | |
| ! | AGTGGAAGAAGAATTGGTCT+TGG | + | chr8.3:17028266-17028285 | None:intergenic | 40.0% |
| !! | AAACCCTAGTTGTTTCTGGA+GGG | + | chr8.3:17027870-17027889 | None:intergenic | 40.0% |
| !! | GGTGCTATTTCTCTTGATAC+TGG | - | chr8.3:17028319-17028338 | MS.gene48393:intron | 40.0% |
| !! | GTGGAAGAAGAATTGGTCTT+GGG | + | chr8.3:17028265-17028284 | None:intergenic | 40.0% |
| AAACCTGAACGATGGTAGCA+TGG | + | chr8.3:17027726-17027745 | None:intergenic | 45.0% | |
| AACCTGAACGATGGTAGCAT+GGG | + | chr8.3:17027725-17027744 | None:intergenic | 45.0% | |
| AAGGATCGCAATGAGGAAGA+TGG | + | chr8.3:17027701-17027720 | None:intergenic | 45.0% | |
| ACCCAAACGCATTGCATCAA+CGG | + | chr8.3:17028218-17028237 | None:intergenic | 45.0% | |
| ACTGTTCATGGTCGTGATCT+TGG | - | chr8.3:17028373-17028392 | MS.gene48393:CDS | 45.0% | |
| CAATTCTTCTTCCACTGCTG+TGG | - | chr8.3:17028270-17028289 | MS.gene48393:intron | 45.0% | |
| CACAGCAGTGGAAGAAGAAT+TGG | + | chr8.3:17028272-17028291 | None:intergenic | 45.0% | |
| CTGTTCATGGTCGTGATCTT+GGG | - | chr8.3:17028374-17028393 | MS.gene48393:CDS | 45.0% | |
| GAAACCCTCCAGAAACAACT+AGG | - | chr8.3:17027863-17027882 | MS.gene48393:CDS | 45.0% | |
| GAAGATGGTGAAGACGATGA+AGG | + | chr8.3:17027686-17027705 | None:intergenic | 45.0% | |
| GGAAAAGGAAACCTGAACGA+TGG | + | chr8.3:17027734-17027753 | None:intergenic | 45.0% | |
| TGTTCATGGTCGTGATCTTG+GGG | - | chr8.3:17028375-17028394 | MS.gene48393:CDS | 45.0% | |
| ! | AATGCGTTTGGGTTTGCACA+AGG | - | chr8.3:17028225-17028244 | MS.gene48393:CDS | 45.0% |
| !! | CAAACCCTAGTTGTTTCTGG+AGG | + | chr8.3:17027871-17027890 | None:intergenic | 45.0% |
| !! | TTGTTTCTGGAGGGTTTCGA+GGG | + | chr8.3:17027861-17027880 | None:intergenic | 45.0% |
| ACCTGAACGATGGTAGCATG+GGG | + | chr8.3:17027724-17027743 | None:intergenic | 50.0% | |
| CAAGCGACGGTTCAACTCTT+TGG | + | chr8.3:17028164-17028183 | None:intergenic | 50.0% | |
| GCCGTTGATGCAATGCGTTT+GGG | - | chr8.3:17028214-17028233 | MS.gene48393:CDS | 50.0% | |
| GGCACAAGATGAGTCGACAT+TGG | + | chr8.3:17028197-17028216 | None:intergenic | 50.0% | |
| GTTTCGAGGGCGGATGAAAA+TGG | + | chr8.3:17027848-17027867 | None:intergenic | 50.0% | |
| TACTGGTAGTTCGGAGTCTG+CGG | - | chr8.3:17028336-17028355 | MS.gene48393:intron | 50.0% | |
| TCTCTTCGGAGAAAGAAGCG+TGG | + | chr8.3:17028054-17028073 | None:intergenic | 50.0% | |
| TCTTCCACTGCTGTGGTTTG+TGG | - | chr8.3:17028277-17028296 | MS.gene48393:intron | 50.0% | |
| TGCCGTTGATGCAATGCGTT+TGG | - | chr8.3:17028213-17028232 | MS.gene48393:CDS | 50.0% | |
| TGGAGATTAGGGACAAGCGA+CGG | + | chr8.3:17028177-17028196 | None:intergenic | 50.0% | |
| ! | CTCTTCGGAGAAAGAAGCGT+GGG | + | chr8.3:17028053-17028072 | None:intergenic | 50.0% |
| ! | GAAGACGATGAAGGTACGAG+TGG | + | chr8.3:17027677-17027696 | None:intergenic | 50.0% |
| ! | GGGCAAACCCTAGTTGTTTC+TGG | + | chr8.3:17027874-17027893 | None:intergenic | 50.0% |
| !! | GTTGTTTCTGGAGGGTTTCG+AGG | + | chr8.3:17027862-17027881 | None:intergenic | 50.0% |
| ACTGGTAGTTCGGAGTCTGC+GGG | - | chr8.3:17028337-17028356 | MS.gene48393:intron | 55.0% | |
| AGCACCACAAACCACAGCAG+TGG | + | chr8.3:17028284-17028303 | None:intergenic | 55.0% | |
| CATGGGGAAGGATCGCAATG+AGG | + | chr8.3:17027708-17027727 | None:intergenic | 55.0% | |
| GAACGATGGTAGCATGGGGA+AGG | + | chr8.3:17027720-17027739 | None:intergenic | 55.0% | |
| GCGACGGTTCAACTCTTTGG+CGG | + | chr8.3:17028161-17028180 | None:intergenic | 55.0% | |
| TCCCCATGCTACCATCGTTC+AGG | - | chr8.3:17027720-17027739 | MS.gene48393:CDS | 55.0% | |
| ! | GCGTTTGGGTTTGCACAAGG+TGG | - | chr8.3:17028228-17028247 | MS.gene48393:CDS | 55.0% |
| ! | TTTCTGGAGGGTTTCGAGGG+CGG | + | chr8.3:17027858-17027877 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 17027667 | 17028414 | 17027667 | ID=MS.gene48393 |
| chr8.3 | mRNA | 17027667 | 17028414 | 17027667 | ID=MS.gene48393.t1;Parent=MS.gene48393 |
| chr8.3 | exon | 17028340 | 17028414 | 17028340 | ID=MS.gene48393.t1.exon1;Parent=MS.gene48393.t1 |
| chr8.3 | CDS | 17028340 | 17028414 | 17028340 | ID=cds.MS.gene48393.t1;Parent=MS.gene48393.t1 |
| chr8.3 | exon | 17028196 | 17028251 | 17028196 | ID=MS.gene48393.t1.exon2;Parent=MS.gene48393.t1 |
| chr8.3 | CDS | 17028196 | 17028251 | 17028196 | ID=cds.MS.gene48393.t1;Parent=MS.gene48393.t1 |
| chr8.3 | exon | 17027667 | 17028072 | 17027667 | ID=MS.gene48393.t1.exon3;Parent=MS.gene48393.t1 |
| chr8.3 | CDS | 17027667 | 17028072 | 17027667 | ID=cds.MS.gene48393.t1;Parent=MS.gene48393.t1 |
| Gene Sequence |
| Protein sequence |
