AlfalfaGEDB | MS.gene50577

M. sativa cultivar XinJiangDaYe / MS.gene50577

KEGG: K00383
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004362 GO:0005488 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009055 GO:0009987 GO:0015036 GO:0015037 GO:0015038 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0019725 GO:0034641 GO:0036094 GO:0042592 GO:0043167 GO:0043168 GO:0043603 GO:0044237 GO:0045454 GO:0048037 GO:0050660 GO:0050661 GO:0050662 GO:0050789 GO:0050794 GO:0051186 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0097159 GO:1901265 GO:1901363 GO:1901564
PFAM: PF02852.25 PF07992.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50577.t1	XP_013468580.1	92	553	44	0	1	553	1	553	1.10E-286	995.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50577.t1	P27456	86.9	549	72	0	5	553	4	552	5.3e-274	944.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50577.t1	A0A072VL94	92.0	553	44	0	1	553	1	553	7.9e-287	995.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049173	MS.gene50577	0.829971	3.82E-55	-1.69E-46
MS.gene049222	MS.gene50577	0.804507	2.09E-49	-1.69E-46
MS.gene049633	MS.gene50577	0.812438	4.24E-51	-1.69E-46
MS.gene050165	MS.gene50577	0.801814	7.54E-49	-1.69E-46
MS.gene050333	MS.gene50577	0.833593	4.88E-56	-1.69E-46
MS.gene050444	MS.gene50577	0.830084	3.58E-55	-1.69E-46
MS.gene05080	MS.gene50577	0.825038	5.83E-54	-1.69E-46
MS.gene050904	MS.gene50577	0.805914	1.06E-49	-1.69E-46
MS.gene051228	MS.gene50577	0.812123	4.97E-51	-1.69E-46
MS.gene051666	MS.gene50577	0.804739	1.87E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene50577	MS.gene019928	PPI
MS.gene50577	MS.gene67036	PPI
MS.gene50577	MS.gene053443	PPI
MS.gene50577	MS.gene073888	PPI
MS.gene50577	MS.gene65554	PPI
MS.gene53517	MS.gene50577	PPI
MS.gene74672	MS.gene50577	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50577.t1	MTR_1g070505	97.830	553	12	0	1	553	1	553	0.0	1112
MS.gene50577.t1	MTR_6g033515	53.878	477	213	5	66	536	16	491	2.70e-180	517
MS.gene50577.t1	MTR_6g033515	52.449	490	212	7	66	536	16	503	2.18e-175	505
MS.gene50577.t1	MTR_4g121880	32.669	502	295	14	48	521	54	540	1.97e-50	182
MS.gene50577.t1	MTR_2g034580	31.504	492	293	14	58	521	68	543	1.14e-48	177
MS.gene50577.t1	MTR_2g034580	31.504	492	293	14	58	521	68	543	2.21e-48	177
MS.gene50577.t1	MTR_4g123070	28.176	433	291	9	104	521	59	486	1.02e-45	168
MS.gene50577.t1	MTR_0644s0030	28.529	333	223	9	116	438	336	663	1.67e-30	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50577.t1	AT3G54660	80.154	519	97	3	36	552	50	564	0.0	876
MS.gene50577.t1	AT3G24170	53.419	468	211	4	72	533	23	489	6.38e-177	509
MS.gene50577.t1	AT3G24170	53.419	468	211	4	72	533	23	489	6.38e-177	509
MS.gene50577.t1	AT3G24170	53.419	468	211	4	72	533	23	489	6.38e-177	509
MS.gene50577.t1	AT3G16950	31.729	561	323	18	3	521	2	544	7.65e-50	181
MS.gene50577.t1	AT3G16950	32.028	562	320	19	3	521	2	544	1.78e-49	181
MS.gene50577.t1	AT4G16155	31.351	555	321	17	3	521	11	541	1.19e-48	177
MS.gene50577.t1	AT1G48030	26.559	433	298	8	104	521	64	491	2.45e-42	159
MS.gene50577.t1	AT1G48030	26.559	433	298	8	104	521	64	491	2.45e-42	159
MS.gene50577.t1	AT1G48030	26.559	433	298	8	104	521	64	491	2.45e-42	159

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Find 150 sgRNAs with CRISPR-Local

Find 363 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCGAGGCAAAGCGAG+AGG	0.642593	1.2:-54118409	None:intergenic
AAACATCGGTTCCTTCAATT+TGG	0.171733	1.2:+54121563	MS.gene50577:CDS
AAACTACTCCAATCATGCTT+AGG	0.489785	1.2:-54120014	None:intergenic
AAACTTCATACCTTAGTATT+AGG	0.232538	1.2:-54120773	None:intergenic
AAAGACCAGCAAAGGTACCG+TGG	0.688890	1.2:+54120712	MS.gene50577:CDS
AAAGATACCAGCAAACTCCA+AGG	0.619374	1.2:-54120429	None:intergenic
AAAGCAATATTGTTAAGTTC+CGG	0.508995	1.2:-54123594	None:intergenic
AACATCGGTTCCTTCAATTT+GGG	0.350449	1.2:+54121564	MS.gene50577:CDS
AACTCCTCCAATTGGTGGTT+GGG	0.383902	1.2:-54122517	None:intergenic
AACTGAAACTAAAATGTGTT+TGG	0.416281	1.2:-54120288	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACACGAGAATTAAGAAAGT+TGG	+	chr1.2:54119078-54119097	MS.gene50577:intron	30.0%
AATCTCATTAACCACGTATT+TGG	-	chr1.2:54119729-54119748	None:intergenic	30.0%
ACAATTTCACTGCAGAATTT+AGG	+	chr1.2:54121396-54121415	MS.gene50577:intron	30.0%
ACCACATGTCATTATTCATA+TGG	+	chr1.2:54123371-54123390	MS.gene50577:intron	30.0%
ACTGAAGTCAATCTTTAATC+AGG	-	chr1.2:54121733-54121752	None:intergenic	30.0%
AGAAGAACACATATATTAGG+AGG	+	chr1.2:54119199-54119218	MS.gene50577:intron	30.0%
AGAATGTAACACAATCTGTT+AGG	-	chr1.2:54122463-54122482	None:intergenic	30.0%
AGATAAACAGACAGTTGAAA+CGG	-	chr1.2:54121326-54121345	None:intergenic	30.0%
AGCAAATTAGCAAGTCAATA+AGG	-	chr1.2:54118850-54118869	None:intergenic	30.0%
AGCGTATGAAATAATTTGGA+GGG	+	chr1.2:54120918-54120937	MS.gene50577:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	54118144	54123604	54118144	ID=MS.gene50577
chr1.2	mRNA	54118144	54123604	54118144	ID=MS.gene50577.t1;Parent=MS.gene50577
chr1.2	exon	54118144	54118505	54118144	ID=MS.gene50577.t1.exon1;Parent=MS.gene50577.t1
chr1.2	CDS	54118144	54118505	54118144	ID=cds.MS.gene50577.t1;Parent=MS.gene50577.t1
chr1.2	exon	54119908	54120133	54119908	ID=MS.gene50577.t1.exon2;Parent=MS.gene50577.t1
chr1.2	CDS	54119908	54120133	54119908	ID=cds.MS.gene50577.t1;Parent=MS.gene50577.t1
chr1.2	exon	54120242	54120520	54120242	ID=MS.gene50577.t1.exon3;Parent=MS.gene50577.t1
chr1.2	CDS	54120242	54120520	54120242	ID=cds.MS.gene50577.t1;Parent=MS.gene50577.t1
chr1.2	exon	54120608	54120784	54120608	ID=MS.gene50577.t1.exon4;Parent=MS.gene50577.t1
chr1.2	CDS	54120608	54120784	54120608	ID=cds.MS.gene50577.t1;Parent=MS.gene50577.t1
chr1.2	exon	54121411	54121464	54121411	ID=MS.gene50577.t1.exon5;Parent=MS.gene50577.t1
chr1.2	CDS	54121411	54121464	54121411	ID=cds.MS.gene50577.t1;Parent=MS.gene50577.t1
chr1.2	exon	54121547	54121695	54121547	ID=MS.gene50577.t1.exon6;Parent=MS.gene50577.t1
chr1.2	CDS	54121547	54121695	54121547	ID=cds.MS.gene50577.t1;Parent=MS.gene50577.t1
chr1.2	exon	54122494	54122557	54122494	ID=MS.gene50577.t1.exon7;Parent=MS.gene50577.t1
chr1.2	CDS	54122494	54122557	54122494	ID=cds.MS.gene50577.t1;Parent=MS.gene50577.t1
chr1.2	exon	54122663	54122833	54122663	ID=MS.gene50577.t1.exon8;Parent=MS.gene50577.t1
chr1.2	CDS	54122663	54122833	54122663	ID=cds.MS.gene50577.t1;Parent=MS.gene50577.t1
chr1.2	exon	54122920	54123057	54122920	ID=MS.gene50577.t1.exon9;Parent=MS.gene50577.t1
chr1.2	CDS	54122920	54123057	54122920	ID=cds.MS.gene50577.t1;Parent=MS.gene50577.t1
chr1.2	exon	54123563	54123604	54123563	ID=MS.gene50577.t1.exon10;Parent=MS.gene50577.t1
chr1.2	CDS	54123563	54123604	54123563	ID=cds.MS.gene50577.t1;Parent=MS.gene50577.t1

Gene Sequence

>MS.gene50577

ATGAAGCAAGCAGCAATGGCTACTTCACTTTCCCTCTCTTGTTCTTCTCCCTTACTTACCCGAAACACTCTCTTCTTCACCAAAACCTTCCCATTCTCTCGCTCCTTCTCTCTCACACCTTCTCTCTCCACCAAAACCCTAATCTCTCAATCCCCTCCCCACCGCACCTTCGCTGTCCGCGCTGAATCGCAAAACGGCGTCGACCCCACCGGCCACTATGACTTCGACCTTTTTACCATCGGTGCCGGAAGCGGAGGTGTCCGTGCCTCTCGCTTTGCCTCGAATTTTGGTGCTAACGCTGCTATCTGTGAGCTTCCGTTCTCCACAATCTCATCGGATACCGCCGGAGGTGTCGGCGGCACGTAAGTTCCTCTTCCATAGCTTGGGGTTTCTTATTCCGTTTTTTAGTTAATTTCACAGTTCGTTGAAAGTGTCCAACCTAGCAATATCGGTATAACCAAATGAGCTACGTTTTACGAACAATTCCAGATGTTGTTAATATGATGTGGGTGTGAAAAATGTACTAATAGTAAGTAAATGAATAGTGCTGTTGTATACATATGTCTATCTAATAATTGTAATGAGAACGTTGTAAAAATGGAATTGGCTAATTACATGATATTATGGTGCTTTGCTTTTTTTTATGCAAATCATAAGAACAAGTGATAGAACTTTAACATGAACATGAGTTTTTACTTTTTTACCTTATTGACTTGCTAATTTGCTCCTGTTCGCTTTGGTTAAACATTGTTGATTTTTTCTCCGTGTCTTTCTGAAAGGAGATGTGTTATTTGAATAACCAACTTTTTAGGTGGTTAATTTAGTTGAAACATGTGGTTAATCATGGTATATGGTTCATGGATATAACATGTGTCTAAACTCATTAACTAATACTACTACCCTTGTCTCTAAATATAGGACCCCATTTGACAAAAACACGAGAATTAAGAAAGTTGGTTGTGATATTAAGTTTGTTGACAGTTAATACTATATTGTTTTAAAATTTTATCCCAATGATTTCATCATAGAGAGCATTTATTATAAATTGTTGTGTTAGAAGAACACATATATTAGGAGGAAGAATTAAGATTTGAACACTAGACTGGTTGGGTTTGTGGTTATTGAAGTTGAAATAAAGATGAAGAACTATATAGAGAAAGTAAAAAAACCATTTTTTTTTTCAGAGAAAAAACTCATTTTTAAGAGAAGCATTTCTTTGGCTTGTTGAAGTGTGGTATTGTGGTAGTTGTTTCTTAACAGAAGATTTTTTGTATTGATTATTAGGAGTGGGTTTACTGAGTCAGGCACAACGAAAGAGATGAGCAATATTTTTTTTGAAGTTTGAATAAAAAAATAAAATCGTGTGTATCTAAAACCAGGGAAGCCGGTTATCCCGTATTTGGTTTTTGGAAAATGGTACTTGGACCAACAGTTTAAAAATTGTTTAAGAGAGAGTTTGAGAGAGGTTGTCTTTAGCATTCCTCTAACTTCAGTAGCTGTTAATTTGAACGTGATTAACCACTACTTTCAAGTCTTTAGATTTATATGTAGTTGAACACCTGAAAATTATGGTTAACCACGCCCAAATACGTGGTTAATGAGATTTGACATCTGATATTATCCATTAACTATCTATTGAATGTAATTAACCACTCATTTAAACTGTGTTGACCACCTTAAAAGTTGTCATGAAAGTTGTTCAAATTTGGTTGTATAAAAAATCAATTCTCTTCTAAAACCATCTACATGCATATCTTTGTGCAGCTGTGTAATACGTGGATGTGTGCCAAAGAAATTACTCGTCTATGCCTCAAAATTTTCTCACGAATTTGAAGAAAGCAATGGTTTTGGATGGAAATATGACAGTGAACCTAAGCATGATTGGAGTAGTTTGATTGCTAATAAAAATGCTGAGTTGCAGAGGCTTACTGGCATCTACAAGAATATTTTGAAAAATTCTAATGTCAAGTTGATTGAAGGCCGTGGAAAGGTGGGATGATTCTTTGGATCCATTTCCATGTAATAAATAGCTTTGTAATTTTAAAAAAGAAAAAAGATCAAATTTTTATTGTGCTGATATTGCTTTTGTAATTCACAGATTGTAGATCCTCACACAGTTGATGTTGATGGGAAGTTATATACAGCCAAACACATTTTAGTTTCAGTTGGAGGTCGTCCCTTCATTCCTGATATTCCTGGAAAGGAATATGCAATAGATTCAGATGCTGCCCTTGATTTACCATCAAAACCTGAGAAGATTGCCATTGTTGGTGGGGGTTACATTGCCTTGGAGTTTGCTGGTATCTTTAATGGTTTGAAAAGCAATGTTCATGTATTTATACGACAAAAGCAGGTTTTGCGAGGATTTGATGAAGAGGTAAGCAAGCATGGACAATACAATGTATTGAATCGTTACTTTGTGTCAACTCTTTCTTCACATTCAAACTATAAATTATGTCTGCAGATTAGAGATTTTGTCGCAGAAAATATGGCTCTAAGAGGTATTGAATTCCATACTGAGGAGTCTCCTGTAGCTATCACTAAGGCAGCTGATGGCTCACTCTCTTTAAAGACCAGCAAAGGTACCGTGGATGGTTTCTCTCATATTATGTTTGCTACAGGACGCAAACCTAATACTAAGGTATGAAGTTTTGATTATGTAGTTTTACTTGTAGTTGACTGTTTCAAAACTAAGTAAAGTTCCAGCAAATACTCTTTTAGCATCACTACCAAAAAAAGATTATAAGCACTACCAAAAAAAAAAATACTCTTTTAGCGTATGAAATAATTTGGAGGGAAGATTTTTATTTGTGCCTTCCAATAATTACCTTCAATCAGAAAGTCATTGAAAAGGACATGAAAAAGAGAATATAATTTATCAAATAAAGATAGTTTAAACATAGTCTTATTTATTCACTTAAATGTCAATATTTTCCCATGATACACTTTTAGGACTTGAAATGTATGTCTTTAAAAAAATGACACACTCCTTCAGCCCCTTGCTTAATATTACATGAATAATTATGTGCTGATGCTGAAAGCTAATTTCCATATTTATCAAAATTAGGAACTATTGAACTCCAGTGTCCTTGAGGGTTGGCTAATTTTAATTCCACATGCTTCTTAGTTGGCTCAATTTCCATACGGCTTGCATGTTCTTGAAGTTTAGTTAAATTTTACCGTTTCAACTGTCTGTTTATCTTATTCATGTTACAGAAAGAGCTAATAACATGGTTGATTCATTTATGAACTACAATTTCACTGCAGAATTTAGGACTGGAGTCTGTTGGTGTGAAGATTGCTAAAGATGGATCAATAGAGGTAGTGTAAGCTTATCATTACTCATTAGTTTCTATATGATAAATATTCATATGATCGCTCAGTGTTACTCCCATTCACCCAGGTTGATGAATACTCTCAAACATCGGTTCCTTCAATTTGGGCAATCGGAGATGCTACAAACAGGATAAATCTCACTCCAGTTGCTTTGATGGAGGGAGTGGCATTAACGAAAACATTGTTTCAGAATGAGCCAACAAAACCTGATTATAGGTAATATACATTGTTTCAGAATGAGACAACAAAACCTGATTAAAGATTGACTTCAGTGACCAGTGTGTGGTTGTATATCATCAACTGACTTCTTAGTCATTATCATAATCCAACTGCTTAACCATTTATAATGATCTCCCTGATCTTGCTTAACAAGCATTAAAAATTGATTTGGTTTTTATTTGTTTGAACTTTGAAAAGTATTATGTTTATCAATCTATTTTTTCGTCTATCAATCTTAGATAATTAGCAAATTGACAATGTGTTTTTGACACGAATTTGTATGGCGAGGTTTATTACACCATTTTTTTTTTAAACTATTACACCATTGTTTCTTAAATGGTCAAGATGCTACAGTTTGTAAATATATGTGTGTAACATCATGACGAGAGCTTTAATGGTTTAAAAGAGGTGGAAAAAGGAAAATGACTAGAGGATTTTTAGCAAAGTTTGATATTTTGGTTTTTGATATGTGTAACTATGTGTGATCGAGCTTGCTGCCGGTGGTTGTTGTAGTTGCTGCGAAGTGCTATTTTTAGCAAAGTTTGATATTTTGGTTTTTGATATGTGTAACTATGTGTGATCGAGCTTGCTGCCGGTGGTTGTTGTAGTTGCTGCGAAGTGCTAACTATGCAAATTTTGTTTATTTTCCTAGTCATCTCATTTTTTGCACATATTTATGATTAAAATTTGTTTCACATTTTCATTTGTAGATATATTGAATGGCAGCTATAATCTGGTCTGCTTTAAGTATTTCATAGCCTCCTAACAGATTGTGTTACATTCTTTCTTTTATAGAGCTGTTCCTGCTGCTGTGTTCTCCCAACCACCAATTGGAGGAGTTGGTCTCACAGAGGAGCAGGTGAATGACCTATGGCAGGCTAAATCCATTGAGATCAATGATCTTCTGAATATTTTGATGAATTATACAAATTATGATTTACTAACATTTTGGTTATTTGCACAGGCTGTAGAACAATATGGCGATATTGACGTCTACACAGCAAATTTTAGGCCACTGAAGGCCACCCTCTCTGGGCTTCCAGACCGGGTTTTTATGAAACTAATAGTGTCTGCAAAAACAAATGAAGTTCTGGGTTTGCACATGTGTGGGGATGATGCTCCTGAAATTGTGCAGGTTTTTGTCTTTACTGCAGTTTTTATATTACTCAGATAATGATACATGAGTATTCTCTTATTTATAAGATTTTGCATGATAAACAGGGGTTTGCAGTTGCTATTAAAGCTGGATTGACAAAGGCAGACTTTGATGCTACTGTAGGCATTCACCCAACCGCAGCTGAGGAGTTTGTCACCATGAGGACTCCCACTAGGAAGATTCGAAAGAGCCTTGATTCACAGGTAAATCAAGGATTCTCAGCCAGTGTCTTCCGTTGATATCTTGTGCAGAAATTATCCTGCATTCTGTTCAGCATACTAAATATTGTGATGATTTCTTAACCATTAATGAATACGTCGACTTCCGTTGCCTTGTACTTGAGAATTGAAAGACTTTAGAATTCTTAAAAAAAAAAAAAAAAAAAAAAACAAAAGAAAACTTCCCTGTGCAGGCCATGATTACTTGGGTCTGTTGATCAAATCAACAGCCTGCTGTCACCCCTTTCCCTCACGAAGAAGTCTAGCAATATGATACTTAATTCATTTGGGTGATAAAACCACATGTCATTATTCATATGGAAATGATATATGGTACAAAATTGGGTACACAAAAGATAGCTTGAAAAACATGTTTTCTTAGTAGTATTTTGTTTTTCATTTCCAACCATGTTTTTATGGTTCTCTTAGCATGCTATTGGTTCTTTTCTAAAAAATTTCGTAATGGTTGTTTTGTTTTAATATATTCTAGGGAAAGCCAGACTCTGAAGCAAAAGCTGTAGCCGGAACTTAA

Protein sequence

>MS.gene50577.t1

MKQAAMATSLSLSCSSPLLTRNTLFFTKTFPFSRSFSLTPSLSTKTLISQSPPHRTFAVRAESQNGVDPTGHYDFDLFTIGAGSGGVRASRFASNFGANAAICELPFSTISSDTAGGVGGTCVIRGCVPKKLLVYASKFSHEFEESNGFGWKYDSEPKHDWSSLIANKNAELQRLTGIYKNILKNSNVKLIEGRGKIVDPHTVDVDGKLYTAKHILVSVGGRPFIPDIPGKEYAIDSDAALDLPSKPEKIAIVGGGYIALEFAGIFNGLKSNVHVFIRQKQVLRGFDEEIRDFVAENMALRGIEFHTEESPVAITKAADGSLSLKTSKGTVDGFSHIMFATGRKPNTKNLGLESVGVKIAKDGSIEVDEYSQTSVPSIWAIGDATNRINLTPVALMEGVALTKTLFQNEPTKPDYRAVPAAVFSQPPIGGVGLTEEQAVEQYGDIDVYTANFRPLKATLSGLPDRVFMKLIVSAKTNEVLGLHMCGDDAPEIVQGFAVAIKAGLTKADFDATVGIHPTAAEEFVTMRTPTRKIRKSLDSQGKPDSEAKAVAGT