AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50857


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50857.t1 MTR_3g005100 100.000 288 0 0 44 331 68 355 0.0 581
MS.gene50857.t1 MTR_3g008040 83.688 282 15 2 50 331 290 540 7.43e-157 450
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50857.t1 AT1G19800 78.276 290 62 1 43 331 61 350 7.57e-170 476
MS.gene50857.t1 AT1G19800 78.276 290 62 1 43 331 61 350 7.57e-170 476
MS.gene50857.t1 AT1G19800 78.276 290 62 1 43 331 61 350 7.57e-170 476
MS.gene50857.t1 AT1G19800 78.276 290 62 1 43 331 61 350 7.57e-170 476
MS.gene50857.t1 AT1G19800 78.276 290 62 1 43 331 61 350 7.57e-170 476
MS.gene50857.t1 AT1G19800 78.276 290 62 1 43 331 61 350 7.57e-170 476

Find 0 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATATTATAAAAGTATAA+AGG + 21095:75708-75727 None:intergenic 10.0%
!! AATTAGCATTAACATTATAT+TGG - 21095:76766-76785 MS.gene50857:CDS 15.0%
!! ATTCGAAATGTGAATTTAAT+AGG - 21095:76081-76100 MS.gene50857:CDS 20.0%
!! ATTTCTTTGATGTACTAAAT+TGG - 21095:75821-75840 MS.gene50857:CDS 20.0%
!!! GTCATTATGAAGATTTTAAA+GGG - 21095:76317-76336 MS.gene50857:CDS 20.0%
! TGAACAAATTGACACATTAA+GGG - 21095:76607-76626 MS.gene50857:intron 25.0%
!! GGTCATTATGAAGATTTTAA+AGG - 21095:76316-76335 MS.gene50857:CDS 25.0%
!! TTATAAAACCGAGGTATTAA+GGG + 21095:75949-75968 None:intergenic 25.0%
!!! GATTTTGAAATTGTGACATA+TGG - 21095:75757-75776 MS.gene50857:CDS 25.0%
!!! TATGAAGATTTTAAAGGGAA+AGG - 21095:76322-76341 MS.gene50857:CDS 25.0%
AACATGGGTGATATAACATT+AGG + 21095:76230-76249 None:intergenic 30.0%
AAGAGAAGCAGAATATATGA+TGG + 21095:75913-75932 None:intergenic 30.0%
ACCCTAATGTAAAACACAAT+AGG + 21095:76709-76728 None:intergenic 30.0%
AGACTCTGTTTGAATTTAAG+AGG + 21095:75851-75870 None:intergenic 30.0%
ATGGGAGAGTTATAAATATG+AGG + 21095:76110-76129 None:intergenic 30.0%
CCCTAATGTAAAACACAATA+GGG + 21095:76708-76727 None:intergenic 30.0%
CGAAATGTGAATTTAATAGG+AGG - 21095:76084-76103 MS.gene50857:CDS 30.0%
CTGAACAAATTGACACATTA+AGG - 21095:76606-76625 MS.gene50857:intron 30.0%
TATTAGTGCAATGATCAAGT+CGG - 21095:76823-76842 MS.gene50857:intron 30.0%
TTAGGATTGAATAGATCTGT+AGG - 21095:76470-76489 MS.gene50857:CDS 30.0%
! ACCCTATTGTGTTTTACATT+AGG - 21095:76704-76723 MS.gene50857:intron 30.0%
! ATTGTGTTTTACATTAGGGT+TGG - 21095:76709-76728 MS.gene50857:intron 30.0%
! CCCTATTGTGTTTTACATTA+GGG - 21095:76705-76724 MS.gene50857:intron 30.0%
! CTGTTTGGTTTAATTGAGAT+GGG + 21095:76128-76147 None:intergenic 30.0%
! GCTATAAAATGTTGTTTGCA+TGG + 21095:75891-75910 None:intergenic 30.0%
! GTTATAAAACCGAGGTATTA+AGG + 21095:75950-75969 None:intergenic 30.0%
! TCTGTTTGGTTTAATTGAGA+TGG + 21095:76129-76148 None:intergenic 30.0%
! TTTTAACATCTGCCTTTGTT+GGG - 21095:76408-76427 MS.gene50857:CDS 30.0%
!! TTTTAAAGGGAAAGGTTCAT+TGG - 21095:76330-76349 MS.gene50857:CDS 30.0%
AAACACAATAGGGTCAAAAG+AGG + 21095:76698-76717 None:intergenic 35.0%
AAACCAACGTTATAAAACCG+AGG + 21095:75958-75977 None:intergenic 35.0%
ATACAAGAAGCAATCACTCT+TGG + 21095:76668-76687 None:intergenic 35.0%
ATATATTCTGCTTCTCTTGC+AGG - 21095:75915-75934 MS.gene50857:CDS 35.0%
ATTAGTGCAATGATCAAGTC+GGG - 21095:76824-76843 MS.gene50857:intron 35.0%
ATTGACACATTAAGGGTTCT+TGG - 21095:76614-76633 MS.gene50857:intron 35.0%
CTTCAACAACTTGAAAGAGT+TGG - 21095:76362-76381 MS.gene50857:CDS 35.0%
CTTCACGATACAATTTGTGA+GGG - 21095:76436-76455 MS.gene50857:CDS 35.0%
GAATCCAATTGAGAGAGATA+AGG + 21095:76065-76084 None:intergenic 35.0%
GACCAATTACTGAGAAACAT+GGG + 21095:76245-76264 None:intergenic 35.0%
GCAGAAACTTAAGCACAAAT+CGG - 21095:76012-76031 MS.gene50857:CDS 35.0%
GCTAATTCCATAAACACCAT+CGG + 21095:76753-76772 None:intergenic 35.0%
GTGATATAACATTAGGAGCA+TGG + 21095:76223-76242 None:intergenic 35.0%
GTGCTTAAGTTTCTGCAATA+TGG + 21095:76008-76027 None:intergenic 35.0%
TGACCAATTACTGAGAAACA+TGG + 21095:76246-76265 None:intergenic 35.0%
TTTGTTCTCCCTTAATACCT+CGG - 21095:75938-75957 MS.gene50857:CDS 35.0%
! CTTTTAACATCTGCCTTTGT+TGG - 21095:76407-76426 MS.gene50857:CDS 35.0%
! TGAAAGAGTTGGTCCAAAAT+CGG - 21095:76373-76392 MS.gene50857:CDS 35.0%
!! ATACCTCGGTTTTATAACGT+TGG - 21095:75952-75971 MS.gene50857:CDS 35.0%
!! CTTTCTTGTTGTTTCTTCCA+AGG - 21095:76983-77002 MS.gene50857:CDS 35.0%
!! TGTCAGTTTTAATCCTAGCA+GGG - 21095:76291-76310 MS.gene50857:CDS 35.0%
!! TTGTCAGTTTTAATCCTAGC+AGG - 21095:76290-76309 MS.gene50857:CDS 35.0%
AATTTGTGAGGGAGTTCACT+AGG - 21095:76447-76466 MS.gene50857:CDS 40.0%
ACAACTATTGCTGTAACCAC+AGG + 21095:76533-76552 None:intergenic 40.0%
ATCTATTGTGAGCTGTGCTT+GGG - 21095:76865-76884 MS.gene50857:intron 40.0%
ATTGAGAGAGATAAGGTGCA+CGG + 21095:76058-76077 None:intergenic 40.0%
CAAACAGAGTCTGTAAGTCA+AGG - 21095:75859-75878 MS.gene50857:CDS 40.0%
CAATAGGGTCAAAAGAGGTA+AGG + 21095:76693-76712 None:intergenic 40.0%
CACAAATCGGAAGAGTAGTT+TGG - 21095:76025-76044 MS.gene50857:CDS 40.0%
CACCCATGTTTCTCAGTAAT+TGG - 21095:76240-76259 MS.gene50857:CDS 40.0%
CCTCACAAATTGTATCGTGA+AGG + 21095:76438-76457 None:intergenic 40.0%
CCTTCACGATACAATTTGTG+AGG - 21095:76435-76454 MS.gene50857:CDS 40.0%
GAAGTAGTAAGATTACCCTG+TGG + 21095:75686-75705 None:intergenic 40.0%
GCTGTTGTGATTTCTCTTGT+TGG - 21095:76938-76957 MS.gene50857:intron 40.0%
GGATTGAATAGATCTGTAGG+CGG - 21095:76473-76492 MS.gene50857:CDS 40.0%
GTGTCACCAGATAATCAACA+GGG + 21095:76646-76665 None:intergenic 40.0%
TAAAGGGAAAGGTTCATTGG+AGG - 21095:76333-76352 MS.gene50857:CDS 40.0%
TATCTATTGTGAGCTGTGCT+TGG - 21095:76864-76883 MS.gene50857:intron 40.0%
TGCACCTTATCTCTCTCAAT+TGG - 21095:76058-76077 MS.gene50857:CDS 40.0%
TGTGTTCATTTCGTCATCCA+TGG + 21095:76198-76217 None:intergenic 40.0%
TTAGTGCAATGATCAAGTCG+GGG - 21095:76825-76844 MS.gene50857:intron 40.0%
! ATCAGACCCTGTTGATTATC+TGG - 21095:76637-76656 MS.gene50857:intron 40.0%
!! AGACATACTCCAACCGATTT+TGG + 21095:76389-76408 None:intergenic 40.0%
!! TTTAGCTCTGGCTTTCTCTA+GGG - 21095:76499-76518 MS.gene50857:intron 40.0%
!!! TTTTAGCTCTGGCTTTCTCT+AGG - 21095:76498-76517 MS.gene50857:intron 40.0%
AAACTGACAATCCCCTCCAT+AGG + 21095:76280-76299 None:intergenic 45.0%
AACAGCCACAGACCTAAACA+CGG - 21095:75657-75676 MS.gene50857:CDS 45.0%
AACATCTGCCTTTGTTGGGA+TGG - 21095:76412-76431 MS.gene50857:CDS 45.0%
AGCAGAACTAGGAACTATGC+AGG - 21095:76580-76599 MS.gene50857:intron 45.0%
ATAAACACCATCGGCGAGTA+GGG + 21095:76744-76763 None:intergenic 45.0%
CATCCATGGTAGAAGCAGTT+GGG + 21095:76184-76203 None:intergenic 45.0%
GCTTGGGGAGTTACAACAAT+GGG - 21095:76881-76900 MS.gene50857:intron 45.0%
GGCAGCACAAACAATCTGTT+TGG + 21095:76143-76162 None:intergenic 45.0%
GTTACAGCAATAGTTGTCGC+CGG - 21095:76536-76555 MS.gene50857:intron 45.0%
TAGTGCAATGATCAAGTCGG+GGG - 21095:76826-76845 MS.gene50857:intron 45.0%
TCATCCATGGTAGAAGCAGT+TGG + 21095:76185-76204 None:intergenic 45.0%
TCTATTGTGAGCTGTGCTTG+GGG - 21095:76866-76885 MS.gene50857:intron 45.0%
TGCTTGGGGAGTTACAACAA+TGG - 21095:76880-76899 MS.gene50857:intron 45.0%
! AGAGTTGGTCCAAAATCGGT+TGG - 21095:76377-76396 MS.gene50857:CDS 45.0%
! AGTGCTTTTGCAGCAGAACT+AGG - 21095:76569-76588 MS.gene50857:intron 45.0%
! GGTGTCACCAGATAATCAAC+AGG + 21095:76647-76666 None:intergenic 45.0%
! TTAGGTCTGTGGCTGTTTCA+TGG + 21095:75654-75673 None:intergenic 45.0%
! TTGTTTCTTCCAAGGAGCTG+GGG - 21095:76991-77010 MS.gene50857:CDS 45.0%
!! ACAACAATGGGAGGTGCTAA+AGG - 21095:76893-76912 MS.gene50857:intron 45.0%
!! AGTTTTAATCCTAGCAGGGC+AGG - 21095:76295-76314 MS.gene50857:CDS 45.0%
!! CTGCAAAAGCACTTCCAATG+CGG + 21095:76562-76581 None:intergenic 45.0%
!! GTTGTTTCTTCCAAGGAGCT+GGG - 21095:76990-77009 MS.gene50857:CDS 45.0%
!! TGTTGTTTCTTCCAAGGAGC+TGG - 21095:76989-77008 MS.gene50857:CDS 45.0%
AATCCCCTCCATAGGTACCT+TGG + 21095:76272-76291 None:intergenic 50.0%
ACAGCCACAGACCTAAACAC+GGG - 21095:75658-75677 MS.gene50857:CDS 50.0%
ACCTAAACACGGGATCCACA+GGG - 21095:75668-75687 MS.gene50857:CDS 50.0%
CATAAACACCATCGGCGAGT+AGG + 21095:76745-76764 None:intergenic 50.0%
CTACTCGCCGATGGTGTTTA+TGG - 21095:76743-76762 MS.gene50857:CDS 50.0%
GGGTGACCATCATCTGTGTT+AGG + 21095:76164-76183 None:intergenic 50.0%
GTGAGGGAGTTCACTAGGTT+AGG - 21095:76452-76471 MS.gene50857:CDS 50.0%
GTGCTGCCTAACACAGATGA+TGG - 21095:76155-76174 MS.gene50857:CDS 50.0%
TCACCCAACTGCTTCTACCA+TGG - 21095:76178-76197 MS.gene50857:CDS 50.0%
TCAGTAATTGGTCACCGCCA+AGG - 21095:76252-76271 MS.gene50857:CDS 50.0%
TCATAATGACCTGCCCTGCT+AGG + 21095:76307-76326 None:intergenic 50.0%
TGGGGAGTTACAACAATGGG+AGG - 21095:76884-76903 MS.gene50857:intron 50.0%
! ATGATCAAGTCGGGGGCTTT+TGG - 21095:76833-76852 MS.gene50857:intron 50.0%
! CTCTAGGGAACTAAGTCCTG+TGG - 21095:76514-76533 MS.gene50857:intron 50.0%
!! AAAAGCACTTCCAATGCGGC+CGG + 21095:76558-76577 None:intergenic 50.0%
!! ATGGGAGGTGCTAAAGGTGT+TGG - 21095:76899-76918 MS.gene50857:intron 50.0%
!!! TGTAGGCGGTGTTTTAGCTC+TGG - 21095:76487-76506 MS.gene50857:intron 50.0%
ACCCTGTGGATCCCGTGTTT+AGG + 21095:75672-75691 None:intergenic 55.0%
CCAGCGATCTCTCAGAGCAT+GGG - 21095:76796-76815 MS.gene50857:intron 55.0%
CGTGAAGGCCATCCCAACAA+AGG + 21095:76423-76442 None:intergenic 55.0%
GACCTAAACACGGGATCCAC+AGG - 21095:75667-75686 MS.gene50857:CDS 55.0%
GGATCCCGTGTTTAGGTCTG+TGG + 21095:75665-75684 None:intergenic 55.0%
TTAGCTGATCCCCAGCTCCT+TGG + 21095:77003-77022 None:intergenic 55.0%
! ACCGCCAAGGTACCTATGGA+GGG - 21095:76265-76284 MS.gene50857:CDS 55.0%
!! CCATGCTCTGAGAGATCGCT+GGG + 21095:76798-76817 None:intergenic 55.0%
ATAGTTGTCGCCGGCCGCAT+TGG - 21095:76545-76564 MS.gene50857:intron 60.0%
CCCAGCGATCTCTCAGAGCA+TGG - 21095:76795-76814 MS.gene50857:intron 60.0%
CCCCTCCATAGGTACCTTGG+CGG + 21095:76269-76288 None:intergenic 60.0%
TCTAGTGCCCTACTCGCCGA+TGG - 21095:76734-76753 MS.gene50857:CDS 60.0%
! CACCGCCAAGGTACCTATGG+AGG - 21095:76264-76283 MS.gene50857:CDS 60.0%
! CCGCCAAGGTACCTATGGAG+GGG - 21095:76266-76285 MS.gene50857:CDS 60.0%
! GGTCACCGCCAAGGTACCTA+TGG - 21095:76261-76280 MS.gene50857:CDS 60.0%
!! CCCATGCTCTGAGAGATCGC+TGG + 21095:76799-76818 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
21095 gene 75631 77036 75631 ID=MS.gene50857
21095 mRNA 75631 77036 75631 ID=MS.gene50857.t1;Parent=MS.gene50857
21095 exon 76978 77036 76978 ID=MS.gene50857.t1.exon1;Parent=MS.gene50857.t1
21095 CDS 76978 77036 76978 ID=cds.MS.gene50857.t1;Parent=MS.gene50857.t1
21095 exon 76731 76801 76731 ID=MS.gene50857.t1.exon2;Parent=MS.gene50857.t1
21095 CDS 76731 76801 76731 ID=cds.MS.gene50857.t1;Parent=MS.gene50857.t1
21095 exon 75631 76496 75631 ID=MS.gene50857.t1.exon3;Parent=MS.gene50857.t1
21095 CDS 75631 76496 75631 ID=cds.MS.gene50857.t1;Parent=MS.gene50857.t1
Gene Sequence

>MS.gene50857

ATGTCAGAGGTAAAAAAATTCCATGAAACAGCCACAGACCTAAACACGGGATCCACAGGGTAATCTTACTACTTCCTTTATACTTTTATAATATGTTCATTTAATCTTATCTTATTTGTTTATCTAGATTTTGAAATTGTGACATATGGATTTTAGAGATTTGATATTTTCAGTCTATCATGCTTTGTATATTTCTTTGATGTACTAAATTGGACTGCCTCTTAAATTCAAACAGAGTCTGTAAGTCAAGGATCAGACCATGCAAACAACATTTTATAGCCATCATATATTCTGCTTCTCTTGCAGGTTTGTTCTCCCTTAATACCTCGGTTTTATAACGTTGGTTTTCTTTAGCAATATTTAATTGACTGTAACCATATTGCAGAAACTTAAGCACAAATCGGAAGAGTAGTTTGGTGAAAATCCGTGCACCTTATCTCTCTCAATTGGATTCGAAATGTGAATTTAATAGGAGGCCTCATATTTATAACTCTCCCATCTCAATTAAACCAAACAGATTGTTTGTGCTGCCTAACACAGATGATGGTCACCCAACTGCTTCTACCATGGATGACGAAATGAACACAAACCATGCTCCTAATGTTATATCACCCATGTTTCTCAGTAATTGGTCACCGCCAAGGTACCTATGGAGGGGATTGTCAGTTTTAATCCTAGCAGGGCAGGTCATTATGAAGATTTTAAAGGGAAAGGTTCATTGGAGGAATACTCTTCAACAACTTGAAAGAGTTGGTCCAAAATCGGTTGGAGTATGTCTTTTAACATCTGCCTTTGTTGGGATGGCCTTCACGATACAATTTGTGAGGGAGTTCACTAGGTTAGGATTGAATAGATCTGTAGGCGGTGTTTTAGCTCTGGCTTTCTCTAGGGAACTAAGTCCTGTGGTTACAGCAATAGTTGTCGCCGGCCGCATTGGAAGTGCTTTTGCAGCAGAACTAGGAACTATGCAGGTTTCTGAACAAATTGACACATTAAGGGTTCTTGGATCAGACCCTGTTGATTATCTGGTGACACCAAGAGTGATTGCTTCTTGTATTGCCTTACCTCTTTTGACCCTATTGTGTTTTACATTAGGGTTGGCATCTAGTGCCCTACTCGCCGATGGTGTTTATGGAATTAGCATTAACATTATATTGGATTCAGCCCAGCGATCTCTCAGAGCATGGGACATTATTAGTGCAATGATCAAGTCGGGGGCTTTTGGTGCTATCATATCTATTGTGAGCTGTGCTTGGGGAGTTACAACAATGGGAGGTGCTAAAGGTGTTGGAGAATCAACAACTTCAGCTGTTGTGATTTCTCTTGTTGGCATCTTCATTGCTGATTTTGCTCTTTCTTGTTGTTTCTTCCAAGGAGCTGGGGATCAGCTAAAGAATTGCGTTTAA

Protein sequence

>MS.gene50857.t1

MSEVKKFHETATDLNTGSTGVCKSRIRPCKQHFIAIIYSASLADDGHPTASTMDDEMNTNHAPNVISPMFLSNWSPPRYLWRGLSVLILAGQVIMKILKGKVHWRNTLQQLERVGPKSVGVCLLTSAFVGMAFTIQFVREFTRLGLNRSVGGVLALAFSRELSPVVTAIVVAGRIGSAFAAELGTMQVSEQIDTLRVLGSDPVDYLVTPRVIASCIALPLLTLLCFTLGLASSALLADGVYGISINIILDSAQRSLRAWDIISAMIKSGAFGAIISIVSCAWGVTTMGGAKGVGESTTSAVVISLVGIFIADFALSCCFFQGAGDQLKNCV