AlfalfaGEDB | MS.gene53535

M. sativa cultivar XinJiangDaYe / MS.gene53535

KEGG: -
GO: -
PFAM: PF04819.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53535.t1	XP_003590149.2	95.6	160	7	0	1	160	132	291	2.30E-80	308.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53535.t1	G7I6L6	95.6	160	7	0	1	160	132	291	1.7e-80	308.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene53535	0.853568	2.19E-61	-1.69E-46
MS.gene049983	MS.gene53535	0.809603	1.74E-50	-1.69E-46
MS.gene050559	MS.gene53535	0.800318	1.52E-48	-1.69E-46
MS.gene051699	MS.gene53535	0.818879	1.56E-52	-1.69E-46
MS.gene052519	MS.gene53535	0.820423	6.91E-53	-1.69E-46
MS.gene052582	MS.gene53535	0.80551	1.29E-49	-1.69E-46
MS.gene052584	MS.gene53535	0.808581	2.89E-50	-1.69E-46
MS.gene052685	MS.gene53535	0.803045	4.20E-49	-1.69E-46
MS.gene053006	MS.gene53535	0.826305	2.92E-54	-1.69E-46
MS.gene053082	MS.gene53535	0.810999	8.72E-51	-1.69E-46
MS.gene053396	MS.gene53535	0.804921	1.71E-49	-1.69E-46
MS.gene053963	MS.gene53535	0.805307	1.42E-49	-1.69E-46
MS.gene054651	MS.gene53535	0.819326	1.23E-52	-1.69E-46
MS.gene055694	MS.gene53535	0.804501	2.10E-49	-1.69E-46
MS.gene055818	MS.gene53535	0.8013	9.61E-49	-1.69E-46
MS.gene055841	MS.gene53535	0.815528	8.83E-52	-1.69E-46
MS.gene056174	MS.gene53535	0.800756	1.24E-48	-1.69E-46
MS.gene056272	MS.gene53535	0.829496	4.98E-55	-1.69E-46
MS.gene056366	MS.gene53535	0.803497	3.39E-49	-1.69E-46
MS.gene056776	MS.gene53535	0.820607	6.27E-53	-1.69E-46
MS.gene057102	MS.gene53535	0.827153	1.83E-54	-1.69E-46
MS.gene057208	MS.gene53535	0.840311	9.38E-58	-1.69E-46
MS.gene058008	MS.gene53535	0.817042	4.05E-52	-1.69E-46
MS.gene058009	MS.gene53535	0.807019	6.20E-50	-1.69E-46
MS.gene058279	MS.gene53535	0.820567	6.41E-53	-1.69E-46
MS.gene058625	MS.gene53535	0.808793	2.60E-50	-1.69E-46
MS.gene058783	MS.gene53535	0.806393	8.41E-50	-1.69E-46
MS.gene05985	MS.gene53535	-0.811246	7.71E-51	-1.69E-46
MS.gene060365	MS.gene53535	0.800853	1.19E-48	-1.69E-46
MS.gene060521	MS.gene53535	0.805069	1.59E-49	-1.69E-46
MS.gene060634	MS.gene53535	-0.80068	1.29E-48	-1.69E-46
MS.gene060935	MS.gene53535	0.811075	8.39E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53535.t1	MTR_1g045060	95.625	160	7	0	1	160	132	291	1.27e-106	306

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 30 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTATTGTTGTGTTGGTTT+TGG	0.203756	1.2:+27990393	MS.gene53535:CDS
GATGTTGTTTATGGTTTGTT+AGG	0.274275	1.2:+27990091	MS.gene53535:CDS
GGTGTTGCTGATGTTGTTTA+TGG	0.290325	1.2:+27990082	MS.gene53535:CDS
TCTTTGTATACTGATTTGTT+TGG	0.292485	1.2:+27990241	MS.gene53535:CDS
GTTATGGATAAAGGTGTTTC+AGG	0.302786	1.2:+27990061	None:intergenic
GAAAGGGTGTAAGAAGATTT+CGG	0.336903	1.2:+27990267	MS.gene53535:CDS
CTTTGTATACTGATTTGTTT+GGG	0.357797	1.2:+27990242	MS.gene53535:CDS
TTGTTTATGGTTTGTTAGGT+GGG	0.410363	1.2:+27990095	MS.gene53535:CDS
TTTACATGCTATTGTTGTGT+TGG	0.447501	1.2:+27990387	MS.gene53535:CDS
CTGATTTGTTTGGGTTGAAA+GGG	0.447568	1.2:+27990251	MS.gene53535:CDS
GAGTATGATGTTATGGATAA+AGG	0.456867	1.2:+27990052	None:intergenic
TTTGAGAGGTGTTGCTATGA+TGG	0.458550	1.2:+27990351	MS.gene53535:CDS
GTTGTTTATGGTTTGTTAGG+TGG	0.474925	1.2:+27990094	MS.gene53535:CDS
ACTGATTTGTTTGGGTTGAA+AGG	0.480665	1.2:+27990250	MS.gene53535:CDS
TGATGTGAAGGGACCGTTGT+TGG	0.496279	1.2:+27990465	MS.gene53535:CDS
TTGGAGTGTTGTCGTGGGGC+AGG	0.501601	1.2:+27990428	MS.gene53535:CDS
ATTGTTTGGAGTGTTGTCGT+GGG	0.540943	1.2:+27990423	MS.gene53535:CDS
TTTGAGAGGTGGTGATGTGA+AGG	0.548157	1.2:+27990453	MS.gene53535:CDS
GATTGTTTGGAGTGTTGTCG+TGG	0.570159	1.2:+27990422	MS.gene53535:CDS
GATGCTTCAGAGATCGAGAT+GGG	0.595874	1.2:+27990520	MS.gene53535:CDS
TCGTGGGGCAGGAATTTGAG+AGG	0.627481	1.2:+27990439	MS.gene53535:CDS
CTTGATGAGGATAGTTTGAG+AGG	0.637571	1.2:+27990337	MS.gene53535:CDS
ATCACACAATCTCATACTCG+TGG	0.648924	1.2:-27990500	None:intergenic
TTGAGAGGTGGTGATGTGAA+GGG	0.669284	1.2:+27990454	MS.gene53535:CDS
TGATGCTTCAGAGATCGAGA+TGG	0.672172	1.2:+27990519	MS.gene53535:CDS
TGGGGCAGGAATTTGAGAGG+TGG	0.673857	1.2:+27990442	MS.gene53535:CDS
ACATCGACATTCTCAATCCG+CGG	0.681866	1.2:-27990304	None:intergenic
GATTCAAGATCAGCCAACAA+CGG	0.697610	1.2:-27990478	None:intergenic
TTGTTTGGAGTGTTGTCGTG+GGG	0.712529	1.2:+27990424	MS.gene53535:CDS
ATGCTTCAGAGATCGAGATG+GGG	0.746310	1.2:+27990521	MS.gene53535:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTTTGTATACTGATTTGTTT+GGG	+	chr1.2:27990242-27990261	MS.gene53535:CDS	25.0%
!!!	TCTTTGTATACTGATTTGTT+TGG	+	chr1.2:27990241-27990260	MS.gene53535:CDS	25.0%
	TTTACATGCTATTGTTGTGT+TGG	+	chr1.2:27990387-27990406	MS.gene53535:CDS	30.0%
!	TGTTTTATGTGATCTTGATG+AGG	+	chr1.2:27990324-27990343	MS.gene53535:CDS	30.0%
!!	GATGTTGTTTATGGTTTGTT+AGG	+	chr1.2:27990091-27990110	MS.gene53535:CDS	30.0%
!!	GTACTTGGTTTTTGCAAATT+GGG	+	chr1.2:27990215-27990234	MS.gene53535:CDS	30.0%
!!	TTGTTTATGGTTTGTTAGGT+GGG	+	chr1.2:27990095-27990114	MS.gene53535:CDS	30.0%
!	GAAAGGGTGTAAGAAGATTT+CGG	+	chr1.2:27990267-27990286	MS.gene53535:CDS	35.0%
!	TAAACCTAATGCGTTTTTCG+CGG	+	chr1.2:27990159-27990178	MS.gene53535:CDS	35.0%
!	TGTTGTGGGATGGTTTTTAA+GGG	+	chr1.2:27990193-27990212	MS.gene53535:CDS	35.0%
!!	ACTGATTTGTTTGGGTTGAA+AGG	+	chr1.2:27990250-27990269	MS.gene53535:CDS	35.0%
!!	CTGATTTGTTTGGGTTGAAA+GGG	+	chr1.2:27990251-27990270	MS.gene53535:CDS	35.0%
!!	GGTACTTGGTTTTTGCAAAT+TGG	+	chr1.2:27990214-27990233	MS.gene53535:CDS	35.0%
!!	GTTGTTTATGGTTTGTTAGG+TGG	+	chr1.2:27990094-27990113	MS.gene53535:CDS	35.0%
!!!	GCTATTGTTGTGTTGGTTTT+GGG	+	chr1.2:27990394-27990413	MS.gene53535:CDS	35.0%
!!!	TGCTATTGTTGTGTTGGTTT+TGG	+	chr1.2:27990393-27990412	MS.gene53535:CDS	35.0%
	ATCACACAATCTCATACTCG+TGG	-	chr1.2:27990503-27990522	None:intergenic	40.0%
	CTTGATGAGGATAGTTTGAG+AGG	+	chr1.2:27990337-27990356	MS.gene53535:CDS	40.0%
	GATTCAAGATCAGCCAACAA+CGG	-	chr1.2:27990481-27990500	None:intergenic	40.0%
!	CGGAGTTTTTCTTGTGTTGT+GGG	+	chr1.2:27990179-27990198	MS.gene53535:CDS	40.0%
!	GTGTTGTGGGATGGTTTTTA+AGG	+	chr1.2:27990192-27990211	MS.gene53535:CDS	40.0%
!	GTTTTTCTTGTGTTGTGGGA+TGG	+	chr1.2:27990183-27990202	MS.gene53535:CDS	40.0%
!!	ATTGTTTGGAGTGTTGTCGT+GGG	+	chr1.2:27990423-27990442	MS.gene53535:CDS	40.0%
!!	GGTGTTGCTGATGTTGTTTA+TGG	+	chr1.2:27990082-27990101	MS.gene53535:CDS	40.0%
!!	GTTTTGGGTCTTGGATTGTT+TGG	+	chr1.2:27990409-27990428	MS.gene53535:CDS	40.0%
!!	TTTGAGAGGTGTTGCTATGA+TGG	+	chr1.2:27990351-27990370	MS.gene53535:CDS	40.0%
!!!	GGATGGTTTTTAAGGGTACT+TGG	+	chr1.2:27990200-27990219	MS.gene53535:CDS	40.0%
	AACTCCGCGAAAAACGCATT+AGG	-	chr1.2:27990166-27990185	None:intergenic	45.0%
	ACATCGACATTCTCAATCCG+CGG	-	chr1.2:27990307-27990326	None:intergenic	45.0%
	ATGCTTCAGAGATCGAGATG+GGG	+	chr1.2:27990521-27990540	MS.gene53535:CDS	45.0%
	GATGCTTCAGAGATCGAGAT+GGG	+	chr1.2:27990520-27990539	MS.gene53535:CDS	45.0%
	TGATGCTTCAGAGATCGAGA+TGG	+	chr1.2:27990519-27990538	MS.gene53535:CDS	45.0%
!	GCGGAGTTTTTCTTGTGTTG+TGG	+	chr1.2:27990178-27990197	MS.gene53535:CDS	45.0%
!	TTGAGAGGTGGTGATGTGAA+GGG	+	chr1.2:27990454-27990473	MS.gene53535:CDS	45.0%
!	TTTGAGAGGTGGTGATGTGA+AGG	+	chr1.2:27990453-27990472	MS.gene53535:CDS	45.0%
!!	GATTGTTTGGAGTGTTGTCG+TGG	+	chr1.2:27990422-27990441	MS.gene53535:CDS	45.0%
!!	TTGTTTGGAGTGTTGTCGTG+GGG	+	chr1.2:27990424-27990443	MS.gene53535:CDS	45.0%
!!!	GTTGTGTTGGTTTTGGGTCT+TGG	+	chr1.2:27990400-27990419	MS.gene53535:CDS	45.0%
	TGATGTGAAGGGACCGTTGT+TGG	+	chr1.2:27990465-27990484	MS.gene53535:CDS	50.0%
!!!	CGGTTTTGAGTTTGAGTCCG+CGG	+	chr1.2:27990287-27990306	MS.gene53535:CDS	50.0%
	TCGTGGGGCAGGAATTTGAG+AGG	+	chr1.2:27990439-27990458	MS.gene53535:CDS	55.0%
	TGGGGCAGGAATTTGAGAGG+TGG	+	chr1.2:27990442-27990461	MS.gene53535:CDS	55.0%
!!	TTGGAGTGTTGTCGTGGGGC+AGG	+	chr1.2:27990428-27990447	MS.gene53535:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	27990064	27990546	27990064	ID=MS.gene53535
chr1.2	mRNA	27990064	27990546	27990064	ID=MS.gene53535.t1;Parent=MS.gene53535
chr1.2	exon	27990064	27990546	27990064	ID=MS.gene53535.t1.exon1;Parent=MS.gene53535.t1
chr1.2	CDS	27990064	27990546	27990064	ID=cds.MS.gene53535.t1;Parent=MS.gene53535.t1

Gene Sequence

>MS.gene53535

ATGGATAAAGGTGTTTCAGGTGTTGCTGATGTTGTTTATGGTTTGTTAGGTGGGTTAACACTTGTGTGTGCTTTTGCATGTTTTTATTTGTCTGTTAAACCTAATGCGTTTTTCGCGGAGTTTTTCTTGTGTTGTGGGATGGTTTTTAAGGGTACTTGGTTTTTGCAAATTGGGTTTTCTTTGTATACTGATTTGTTTGGGTTGAAAGGGTGTAAGAAGATTTCGGTTTTGAGTTTGAGTCCGCGGATTGAGAATGTCGATGTTTTATGTGATCTTGATGAGGATAGTTTGAGAGGTGTTGCTATGATGGAGCTTTTGTTTATTTTACATGCTATTGTTGTGTTGGTTTTGGGTCTTGGATTGTTTGGAGTGTTGTCGTGGGGCAGGAATTTGAGAGGTGGTGATGTGAAGGGACCGTTGTTGGCTGATCTTGAATCCACGAGTATGAGATTGTGTGATGCTTCAGAGATCGAGATGGGGTGA

Protein sequence

>MS.gene53535.t1

MDKGVSGVADVVYGLLGGLTLVCAFACFYLSVKPNAFFAEFFLCCGMVFKGTWFLQIGFSLYTDLFGLKGCKKISVLSLSPRIENVDVLCDLDEDSLRGVAMMELLFILHAIVVLVLGLGLFGVLSWGRNLRGGDVKGPLLADLESTSMRLCDASEIEMG