AlfalfaGEDB | MS.gene55619

M. sativa cultivar XinJiangDaYe / MS.gene55619

KEGG: K02885
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01280.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55619.t1	KEH43214.1	100	159	0	0	1	159	34	192	3.90E-76	294.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55619.t1	P49693	91.8	159	13	0	1	159	34	192	2.2e-72	273.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55619.t1	A0A072VMH3	100.0	159	0	0	1	159	34	192	2.8e-76	294.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049387	MS.gene55619	0.849695	2.74E-60	-1.69E-46
MS.gene050134	MS.gene55619	0.910508	1.73E-82	-1.69E-46
MS.gene050252	MS.gene55619	0.842001	3.37E-58	-1.69E-46
MS.gene050791	MS.gene55619	0.83464	2.67E-56	-1.69E-46
MS.gene051904	MS.gene55619	0.810154	1.33E-50	-1.69E-46
MS.gene051925	MS.gene55619	0.85404	1.60E-61	-1.69E-46
MS.gene051927	MS.gene55619	0.801355	9.36E-49	-1.69E-46
MS.gene052664	MS.gene55619	0.866275	3.21E-65	-1.69E-46
MS.gene052666	MS.gene55619	0.912929	1.11E-83	-1.69E-46
MS.gene053224	MS.gene55619	0.894081	3.45E-75	-1.69E-46
MS.gene054624	MS.gene55619	0.808206	3.47E-50	-1.69E-46
MS.gene057272	MS.gene55619	0.914341	2.15E-84	-1.69E-46
MS.gene057321	MS.gene55619	0.802193	6.30E-49	-1.69E-46
MS.gene057641	MS.gene55619	0.845856	3.13E-59	-1.69E-46
MS.gene057643	MS.gene55619	0.906521	1.36E-80	-1.69E-46
MS.gene059340	MS.gene55619	0.851108	1.10E-60	-1.69E-46
MS.gene060432	MS.gene55619	0.824992	5.98E-54	-1.69E-46
MS.gene060483	MS.gene55619	0.87301	2.05E-67	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55619.t1	MTR_1g087910	98.889	180	2	0	1	180	34	213	8.17e-127	355
MS.gene55619.t1	MTR_1g087910	98.844	173	2	0	1	173	34	206	2.66e-122	343
MS.gene55619.t1	MTR_7g108955	92.857	182	11	1	1	180	34	215	2.36e-106	303
MS.gene55619.t1	MTR_7g108955	93.143	175	10	1	1	173	34	208	1.21e-102	294

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55619.t1	AT3G16780	84.571	175	25	1	1	173	34	208	3.59e-102	293
MS.gene55619.t1	AT4G02230	86.667	180	19	1	1	180	34	208	3.89e-93	270
MS.gene55619.t1	AT1G02780	94.118	153	9	0	1	153	34	186	5.30e-92	267
MS.gene55619.t1	AT4G16030	42.384	151	32	9	24	162	6	113	1.55e-15	69.3

Find 66 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGATACTTGATTTACTTCT+TGG	0.183729	6.2:+19326091	None:intergenic
CCTCCTCATCCAAAGGATTT+TGG	0.205932	6.2:+19326451	None:intergenic
TACTTCTTGGATTTCTTTGA+TGG	0.223836	6.2:+19326104	None:intergenic
TAACCAACTTCCTGATGTTT+TGG	0.279942	6.2:+19326609	None:intergenic
CTTGGATTTCTTTGATGGTT+GGG	0.283634	6.2:+19326109	None:intergenic
GCTGTTGCAGCTGGTGCGGC+TGG	0.322299	6.2:+19326140	None:intergenic
CTCTCTAGCCTTCTCAGCCT+TGG	0.342124	6.2:+19326289	None:intergenic
ATTTCTTTGATGGTTGGGAC+TGG	0.351728	6.2:+19326114	None:intergenic
GCGGCTGGTGCTGCAGCTGC+AGG	0.351870	6.2:+19326155	None:intergenic
GGCCAAGAGGGCGAAGAATA+AGG	0.353398	6.2:-19326243	MS.gene55619:CDS
GACTGGGTTGCTGTTGCAGC+TGG	0.363015	6.2:+19326131	None:intergenic
CAAGAACAAGAGAGTCCTTA+TGG	0.366020	6.2:-19326327	MS.gene55619:CDS
TTTCTTTGATGGTTGGGACT+GGG	0.381319	6.2:+19326115	None:intergenic
AAGGGACGTCATTCTGGATA+TGG	0.390520	6.2:-19326509	MS.gene55619:CDS
AAGAGGAAGGGACGTCATTC+TGG	0.393970	6.2:-19326515	MS.gene55619:CDS
TCTTGGATTTCTTTGATGGT+TGG	0.398574	6.2:+19326108	None:intergenic
GGCTCCCCACCAAAATCCTT+TGG	0.400346	6.2:-19326460	MS.gene55619:CDS
AGTGAATCTTAGTTGGCTTC+CGG	0.400791	6.2:+19326570	None:intergenic
AGCCTTATTCTTCGCCCTCT+TGG	0.400876	6.2:+19326241	None:intergenic
TGTCAATCTTCTTTGCCTCT+CGG	0.425253	6.2:+19326396	None:intergenic
TTAAGGATGGCTTCATTATC+CGG	0.428777	6.2:-19326589	MS.gene55619:CDS
GAGCGCTTTGCTCAAGGTCC+AGG	0.439836	6.2:-19326188	MS.gene55619:CDS
GCTGCAGGAACCTTCTCTCC+TGG	0.459743	6.2:+19326170	None:intergenic
AAACATCAGGAAGTTGGTTA+AGG	0.466451	6.2:-19326606	MS.gene55619:CDS
TTCTGGATATGGTAAAAGAA+AGG	0.467098	6.2:-19326498	MS.gene55619:CDS
AGCAAAGCGCTCTTCTCTGC+GGG	0.467270	6.2:+19326199	None:intergenic
CCAATTCACGCCAAAACATC+AGG	0.468840	6.2:-19326619	MS.gene55619:CDS
GACATTATCTGATCAGTTTG+AGG	0.469564	6.2:-19326264	MS.gene55619:CDS
GGGCGAAGAATAAGGCTAGC+AGG	0.473984	6.2:-19326235	MS.gene55619:CDS
TGAGGAGGATGCGTGTCCTC+AGG	0.478520	6.2:-19326436	MS.gene55619:CDS
GAGCGGGAGTGAATCTTAGT+TGG	0.479570	6.2:+19326563	None:intergenic
TTCACTCCCGCTCCCGTGCT+AGG	0.486394	6.2:-19326553	MS.gene55619:CDS
CTCCTTCATACGCCTAGCAC+GGG	0.507928	6.2:+19326541	None:intergenic
TCTGGATATGGTAAAAGAAA+GGG	0.513865	6.2:-19326497	MS.gene55619:CDS
AGAATGACGTCCCTTCCTCT+TGG	0.519292	6.2:+19326517	None:intergenic
CTCCCGTGCTAGGCGTATGA+AGG	0.520929	6.2:-19326543	MS.gene55619:CDS
ACGCCAAAACATCAGGAAGT+TGG	0.521307	6.2:-19326612	MS.gene55619:CDS
TGCTCAAGGTCCAGGAGAGA+AGG	0.524304	6.2:-19326180	MS.gene55619:CDS
CCTCCTTCATACGCCTAGCA+CGG	0.532368	6.2:+19326540	None:intergenic
ACTTGTGAATACTCTCCATA+AGG	0.533671	6.2:+19326312	None:intergenic
ATCAGGAAGTTGGTTAAGGA+TGG	0.541752	6.2:-19326602	MS.gene55619:CDS
TCACAAGTCCAAGGCTGAGA+AGG	0.542917	6.2:-19326297	MS.gene55619:CDS
CATACGCCTAGCACGGGAGC+GGG	0.545913	6.2:+19326547	None:intergenic
CCACCAAAATCCTTTGGATG+AGG	0.555880	6.2:-19326454	MS.gene55619:CDS
TATGAAGGAGGCCAAGAGGA+AGG	0.560584	6.2:-19326528	MS.gene55619:CDS
AGAGAAGAGCGCTTTGCTCA+AGG	0.563109	6.2:-19326194	MS.gene55619:CDS
TGATATGTACATGAAGGTGA+AGG	0.563752	6.2:-19326360	MS.gene55619:CDS
GAGCAAAGCGCTCTTCTCTG+CGG	0.564950	6.2:+19326198	None:intergenic
TCATACGCCTAGCACGGGAG+CGG	0.568186	6.2:+19326546	None:intergenic
CCAAAATCCTTTGGATGAGG+AGG	0.569919	6.2:-19326451	MS.gene55619:CDS
GGCGAAGAATAAGGCTAGCA+GGG	0.575881	6.2:-19326234	MS.gene55619:CDS
AGAATAAGGCTAGCAGGGAA+AGG	0.589605	6.2:-19326229	MS.gene55619:CDS
TGATCAGTTTGAGGCCAAGA+GGG	0.598337	6.2:-19326255	MS.gene55619:CDS
CCGTGCTAGGCGTATGAAGG+AGG	0.607212	6.2:-19326540	MS.gene55619:CDS
CACGCATCCTCCTCATCCAA+AGG	0.610691	6.2:+19326444	None:intergenic
GAAAGGGTACAAGAGAGGCT+AGG	0.611551	6.2:-19326481	MS.gene55619:CDS
GATATGTACATGAAGGTGAA+GGG	0.614061	6.2:-19326359	MS.gene55619:CDS
ATGAAGGAGGCCAAGAGGAA+GGG	0.617125	6.2:-19326527	MS.gene55619:CDS
GGAGAGTATTCACAAGTCCA+AGG	0.617146	6.2:-19326306	MS.gene55619:CDS
GGTTGCTGTTGCAGCTGGTG+CGG	0.620402	6.2:+19326136	None:intergenic
CTGATCAGTTTGAGGCCAAG+AGG	0.627289	6.2:-19326256	MS.gene55619:CDS
GGCGTATGAAGGAGGCCAAG+AGG	0.634053	6.2:-19326532	MS.gene55619:CDS
TAAAAGAAAGGGTACAAGAG+AGG	0.643495	6.2:-19326486	MS.gene55619:CDS
GTATCATGATATGTACATGA+AGG	0.691105	6.2:-19326366	MS.gene55619:CDS
TTTGCTTCGCAAGTACCGAG+AGG	0.708306	6.2:-19326411	MS.gene55619:CDS
ACTTGCGAAGCAAACGCCTG+AGG	0.708807	6.2:+19326420	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTATCATGATATGTACATGA+AGG	-	chr6.2:19326360-19326379	MS.gene55619:CDS	30.0%
	TACTTCTTGGATTTCTTTGA+TGG	+	chr6.2:19326625-19326644	None:intergenic	30.0%
	TCTGGATATGGTAAAAGAAA+GGG	-	chr6.2:19326229-19326248	MS.gene55619:CDS	30.0%
	TTCTGGATATGGTAAAAGAA+AGG	-	chr6.2:19326228-19326247	MS.gene55619:CDS	30.0%
	ACTTGTGAATACTCTCCATA+AGG	+	chr6.2:19326417-19326436	None:intergenic	35.0%
	GACATTATCTGATCAGTTTG+AGG	-	chr6.2:19326462-19326481	MS.gene55619:CDS	35.0%
	GATATGTACATGAAGGTGAA+GGG	-	chr6.2:19326367-19326386	MS.gene55619:CDS	35.0%
	TGATATGTACATGAAGGTGA+AGG	-	chr6.2:19326366-19326385	MS.gene55619:CDS	35.0%
	TTAAGGATGGCTTCATTATC+CGG	-	chr6.2:19326137-19326156	MS.gene55619:CDS	35.0%
!	AAACATCAGGAAGTTGGTTA+AGG	-	chr6.2:19326120-19326139	MS.gene55619:CDS	35.0%
!	CTTGGATTTCTTTGATGGTT+GGG	+	chr6.2:19326620-19326639	None:intergenic	35.0%
!	TAAAAGAAAGGGTACAAGAG+AGG	-	chr6.2:19326240-19326259	MS.gene55619:CDS	35.0%
!	TAACCAACTTCCTGATGTTT+TGG	+	chr6.2:19326120-19326139	None:intergenic	35.0%
!	TCTTGGATTTCTTTGATGGT+TGG	+	chr6.2:19326621-19326640	None:intergenic	35.0%
	CAAGAACAAGAGAGTCCTTA+TGG	-	chr6.2:19326399-19326418	MS.gene55619:CDS	40.0%
	TGTCAATCTTCTTTGCCTCT+CGG	+	chr6.2:19326333-19326352	None:intergenic	40.0%
!	AGTGAATCTTAGTTGGCTTC+CGG	+	chr6.2:19326159-19326178	None:intergenic	40.0%
!	ATCAGGAAGTTGGTTAAGGA+TGG	-	chr6.2:19326124-19326143	MS.gene55619:CDS	40.0%
!	ATTTCTTTGATGGTTGGGAC+TGG	+	chr6.2:19326615-19326634	None:intergenic	40.0%
!	TTTCTTTGATGGTTGGGACT+GGG	+	chr6.2:19326614-19326633	None:intergenic	40.0%
!!	CTCATCCAAAGGATTTTGGT+GGG	+	chr6.2:19326274-19326293	None:intergenic	40.0%
!!	TCATCCAAAGGATTTTGGTG+GGG	+	chr6.2:19326273-19326292	None:intergenic	40.0%
	AAGGGACGTCATTCTGGATA+TGG	-	chr6.2:19326217-19326236	MS.gene55619:CDS	45.0%
	ACGCCAAAACATCAGGAAGT+TGG	-	chr6.2:19326114-19326133	MS.gene55619:CDS	45.0%
	AGAATAAGGCTAGCAGGGAA+AGG	-	chr6.2:19326497-19326516	MS.gene55619:CDS	45.0%
	CCAAAATCCTTTGGATGAGG+AGG	-	chr6.2:19326275-19326294	MS.gene55619:CDS	45.0%
	CCAATTCACGCCAAAACATC+AGG	-	chr6.2:19326107-19326126	MS.gene55619:CDS	45.0%
	CCACCAAAATCCTTTGGATG+AGG	-	chr6.2:19326272-19326291	MS.gene55619:CDS	45.0%
!	CCTCATCCAAAGGATTTTGG+TGG	+	chr6.2:19326275-19326294	None:intergenic	45.0%
!	CCTCCTCATCCAAAGGATTT+TGG	+	chr6.2:19326278-19326297	None:intergenic	45.0%
!	TGATCAGTTTGAGGCCAAGA+GGG	-	chr6.2:19326471-19326490	MS.gene55619:CDS	45.0%
!!	GGAGAGTATTCACAAGTCCA+AGG	-	chr6.2:19326420-19326439	MS.gene55619:CDS	45.0%
!!!	CCTGATGTTTTGGCGTGAAT+TGG	+	chr6.2:19326110-19326129	None:intergenic	45.0%
	AAGAGGAAGGGACGTCATTC+TGG	-	chr6.2:19326211-19326230	MS.gene55619:CDS	50.0%
	AGAATGACGTCCCTTCCTCT+TGG	+	chr6.2:19326212-19326231	None:intergenic	50.0%
	ATGAAGGAGGCCAAGAGGAA+GGG	-	chr6.2:19326199-19326218	MS.gene55619:CDS	50.0%
	GAGCGGGAGTGAATCTTAGT+TGG	+	chr6.2:19326166-19326185	None:intergenic	50.0%
	GGCGAAGAATAAGGCTAGCA+GGG	-	chr6.2:19326492-19326511	MS.gene55619:CDS	50.0%
	TATGAAGGAGGCCAAGAGGA+AGG	-	chr6.2:19326198-19326217	MS.gene55619:CDS	50.0%
	TCACAAGTCCAAGGCTGAGA+AGG	-	chr6.2:19326429-19326448	MS.gene55619:CDS	50.0%
!	CTGATCAGTTTGAGGCCAAG+AGG	-	chr6.2:19326470-19326489	MS.gene55619:CDS	50.0%
!	GAAAGGGTACAAGAGAGGCT+AGG	-	chr6.2:19326245-19326264	MS.gene55619:CDS	50.0%
!!	AGAGAAGAGCGCTTTGCTCA+AGG	-	chr6.2:19326532-19326551	MS.gene55619:CDS	50.0%
!!	AGCCTTATTCTTCGCCCTCT+TGG	+	chr6.2:19326488-19326507	None:intergenic	50.0%
!!	TTTGCTTCGCAAGTACCGAG+AGG	-	chr6.2:19326315-19326334	MS.gene55619:CDS	50.0%
	ACTTGCGAAGCAAACGCCTG+AGG	+	chr6.2:19326309-19326328	None:intergenic	55.0%
	CACGCATCCTCCTCATCCAA+AGG	+	chr6.2:19326285-19326304	None:intergenic	55.0%
	CCTCCTTCATACGCCTAGCA+CGG	+	chr6.2:19326189-19326208	None:intergenic	55.0%
	CTCCTTCATACGCCTAGCAC+GGG	+	chr6.2:19326188-19326207	None:intergenic	55.0%
	GGCCAAGAGGGCGAAGAATA+AGG	-	chr6.2:19326483-19326502	MS.gene55619:CDS	55.0%
	GGCTCCCCACCAAAATCCTT+TGG	-	chr6.2:19326266-19326285	MS.gene55619:CDS	55.0%
	GGGCGAAGAATAAGGCTAGC+AGG	-	chr6.2:19326491-19326510	MS.gene55619:CDS	55.0%
	TGCTCAAGGTCCAGGAGAGA+AGG	-	chr6.2:19326546-19326565	MS.gene55619:CDS	55.0%
!	AGCAAAGCGCTCTTCTCTGC+GGG	+	chr6.2:19326530-19326549	None:intergenic	55.0%
!	CTCTCTAGCCTTCTCAGCCT+TGG	+	chr6.2:19326440-19326459	None:intergenic	55.0%
!	GAGCAAAGCGCTCTTCTCTG+CGG	+	chr6.2:19326531-19326550	None:intergenic	55.0%
	CCGTGCTAGGCGTATGAAGG+AGG	-	chr6.2:19326186-19326205	MS.gene55619:CDS	60.0%
	CTCCCGTGCTAGGCGTATGA+AGG	-	chr6.2:19326183-19326202	MS.gene55619:CDS	60.0%
	GAGCGCTTTGCTCAAGGTCC+AGG	-	chr6.2:19326538-19326557	MS.gene55619:CDS	60.0%
	GCTGCAGGAACCTTCTCTCC+TGG	+	chr6.2:19326559-19326578	None:intergenic	60.0%
	GGCGTATGAAGGAGGCCAAG+AGG	-	chr6.2:19326194-19326213	MS.gene55619:CDS	60.0%
	TCATACGCCTAGCACGGGAG+CGG	+	chr6.2:19326183-19326202	None:intergenic	60.0%
	TGAGGAGGATGCGTGTCCTC+AGG	-	chr6.2:19326290-19326309	MS.gene55619:CDS	60.0%
!	GACTGGGTTGCTGTTGCAGC+TGG	+	chr6.2:19326598-19326617	None:intergenic	60.0%
!	GGTTGCTGTTGCAGCTGGTG+CGG	+	chr6.2:19326593-19326612	None:intergenic	60.0%
	CATACGCCTAGCACGGGAGC+GGG	+	chr6.2:19326182-19326201	None:intergenic	65.0%
	TTCACTCCCGCTCCCGTGCT+AGG	-	chr6.2:19326173-19326192	MS.gene55619:CDS	65.0%
	GCTGTTGCAGCTGGTGCGGC+TGG	+	chr6.2:19326589-19326608	None:intergenic	70.0%
!!	GCGGCTGGTGCTGCAGCTGC+AGG	+	chr6.2:19326574-19326593	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	19326103	19326645	19326103	ID=MS.gene55619
chr6.2	mRNA	19326103	19326645	19326103	ID=MS.gene55619.t1;Parent=MS.gene55619
chr6.2	exon	19326103	19326645	19326103	ID=MS.gene55619.t1.exon1;Parent=MS.gene55619.t1
chr6.2	CDS	19326103	19326645	19326103	ID=cds.MS.gene55619.t1;Parent=MS.gene55619.t1

Gene Sequence

>MS.gene55619

ATGGCCAATTCACGCCAAAACATCAGGAAGTTGGTTAAGGATGGCTTCATTATCCGGAAGCCAACTAAGATTCACTCCCGCTCCCGTGCTAGGCGTATGAAGGAGGCCAAGAGGAAGGGACGTCATTCTGGATATGGTAAAAGAAAGGGTACAAGAGAGGCTAGGCTCCCCACCAAAATCCTTTGGATGAGGAGGATGCGTGTCCTCAGGCGTTTGCTTCGCAAGTACCGAGAGGCAAAGAAGATTGACAAACATATGTATCATGATATGTACATGAAGGTGAAGGGAAATGTCTTCAAGAACAAGAGAGTCCTTATGGAGAGTATTCACAAGTCCAAGGCTGAGAAGGCTAGAGAGAAGACATTATCTGATCAGTTTGAGGCCAAGAGGGCGAAGAATAAGGCTAGCAGGGAAAGGAAGCATGCCCGCAGAGAAGAGCGCTTTGCTCAAGGTCCAGGAGAGAAGGTTCCTGCAGCTGCAGCACCAGCCGCACCAGCTGCAACAGCAACCCAGTCCCAACCATCAAAGAAATCCAAGAAGTAA

Protein sequence

>MS.gene55619.t1

MANSRQNIRKLVKDGFIIRKPTKIHSRSRARRMKEAKRKGRHSGYGKRKGTREARLPTKILWMRRMRVLRRLLRKYREAKKIDKHMYHDMYMKVKGNVFKNKRVLMESIHKSKAEKAREKTLSDQFEAKRAKNKASRERKHARREERFAQGPGEKVPAAAAPAAPAATATQSQPSKKSKK