Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55785.t1 | XP_013447177.1 | 93.4 | 181 | 12 | 0 | 1 | 181 | 1 | 181 | 6.50E-87 | 330.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55785.t1 | A0A072TUK3 | 93.4 | 181 | 12 | 0 | 1 | 181 | 1 | 181 | 4.7e-87 | 330.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051234 | MS.gene55785 | -0.819014 | 1.45E-52 | -1.69E-46 |
| MS.gene052155 | MS.gene55785 | 0.803563 | 3.28E-49 | -1.69E-46 |
| MS.gene053272 | MS.gene55785 | 0.809281 | 2.05E-50 | -1.69E-46 |
| MS.gene053495 | MS.gene55785 | 0.814659 | 1.38E-51 | -1.69E-46 |
| MS.gene054004 | MS.gene55785 | -0.802914 | 4.47E-49 | -1.69E-46 |
| MS.gene054939 | MS.gene55785 | -0.800149 | 1.65E-48 | -1.69E-46 |
| MS.gene054940 | MS.gene55785 | -0.814074 | 1.85E-51 | -1.69E-46 |
| MS.gene055221 | MS.gene55785 | -0.801985 | 6.95E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene55785 | -0.820458 | 6.79E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene55785 | -0.806163 | 9.40E-50 | -1.69E-46 |
| MS.gene057148 | MS.gene55785 | 0.828074 | 1.10E-54 | -1.69E-46 |
| MS.gene057684 | MS.gene55785 | 0.821886 | 3.18E-53 | -1.69E-46 |
| MS.gene059026 | MS.gene55785 | 0.814168 | 1.77E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene55785 | -0.816609 | 5.06E-52 | -1.69E-46 |
| MS.gene059778 | MS.gene55785 | -0.852339 | 4.93E-61 | -1.69E-46 |
| MS.gene05985 | MS.gene55785 | 0.823991 | 1.03E-53 | -1.69E-46 |
| MS.gene059900 | MS.gene55785 | 0.813173 | 2.93E-51 | -1.69E-46 |
| MS.gene059901 | MS.gene55785 | 0.843153 | 1.67E-58 | -1.69E-46 |
| MS.gene060338 | MS.gene55785 | -0.818653 | 1.75E-52 | -1.69E-46 |
| MS.gene060937 | MS.gene55785 | -0.808485 | 3.03E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene55785 | -0.814124 | 1.81E-51 | -1.69E-46 |
| MS.gene061188 | MS.gene55785 | -0.827673 | 1.37E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55785.t1 | MTR_8g099345 | 93.370 | 181 | 12 | 0 | 1 | 181 | 1 | 181 | 1.91e-122 | 343 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55785.t1 | AT1G64355 | 75.556 | 135 | 26 | 3 | 32 | 162 | 43 | 174 | 5.91e-66 | 201 |
| MS.gene55785.t1 | AT1G64355 | 66.990 | 103 | 25 | 4 | 32 | 128 | 43 | 142 | 7.14e-37 | 125 |
| MS.gene55785.t1 | AT1G64355 | 63.158 | 95 | 28 | 3 | 32 | 122 | 43 | 134 | 1.56e-31 | 111 |
Find 45 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAGTTCCAGCTGGAATTTA+TGG | 0.258898 | 4.3:-4757728 | MS.gene55785:intron |
| GGTGAGGTTAGGAGAAGTTT+TGG | 0.272109 | 4.3:+4757886 | None:intergenic |
| TTGAAGGGTTGATAAGTTTA+GGG | 0.291880 | 4.3:+4758116 | None:intergenic |
| GTTGAAGGGTTGATAAGTTT+AGG | 0.306773 | 4.3:+4758115 | None:intergenic |
| CACTAACTTGTTCATTGTGT+TGG | 0.314311 | 4.3:-4757362 | MS.gene55785:CDS |
| TTAAATGAAAATCACGTTGC+AGG | 0.357937 | 4.3:+4758086 | None:intergenic |
| ACTAACTTGTTCATTGTGTT+GGG | 0.374119 | 4.3:-4757361 | MS.gene55785:CDS |
| TTGACAGCTAAGGTGCATTA+TGG | 0.386555 | 4.3:-4757442 | MS.gene55785:intron |
| TGCCCCTGGGTGTTGATCCA+TGG | 0.395407 | 4.3:-4758000 | MS.gene55785:CDS |
| AATCAGAAAAGCTGGAAATT+CGG | 0.416724 | 4.3:-4757326 | MS.gene55785:CDS |
| TCTCCTAACCTCACCCTCTT+CGG | 0.418605 | 4.3:-4757878 | MS.gene55785:CDS |
| AAACCGAAGAGGGTGAGGTT+AGG | 0.440423 | 4.3:+4757875 | None:intergenic |
| GATCGTGTGTGGATTGCCCC+TGG | 0.440978 | 4.3:-4758014 | MS.gene55785:CDS |
| TCTAATGTGGATTGGTTACA+TGG | 0.443829 | 4.3:-4757409 | MS.gene55785:CDS |
| CTTCTTTGTCTAATGTGGAT+TGG | 0.447286 | 4.3:-4757417 | MS.gene55785:CDS |
| ATGTGGATTGGTTACATGGT+GGG | 0.450371 | 4.3:-4757405 | MS.gene55785:CDS |
| TCGAAGGAGGAGAAGAAACC+TGG | 0.458784 | 4.3:-4757289 | MS.gene55785:CDS |
| TGGAACTTCTTTGTCTAATG+TGG | 0.464776 | 4.3:-4757422 | MS.gene55785:CDS |
| AGAGAGGGAATCAGAAAAGC+TGG | 0.466045 | 4.3:-4757334 | MS.gene55785:CDS |
| GCCCCTGGGTGTTGATCCAT+GGG | 0.473465 | 4.3:-4757999 | MS.gene55785:CDS |
| CGTTGCAGGAAGGTCGTTGA+AGG | 0.474175 | 4.3:+4758100 | None:intergenic |
| ATTGTGTTGGGTTTGAGAGA+GGG | 0.496509 | 4.3:-4757349 | MS.gene55785:CDS |
| AGCGATGCTCTGTGAATCAA+TGG | 0.506212 | 4.3:+4757970 | None:intergenic |
| GGAGAAGAAACCTGGGATGA+AGG | 0.519084 | 4.3:-4757281 | MS.gene55785:CDS |
| GACTCACCATAAATTCCAGC+TGG | 0.525068 | 4.3:+4757722 | None:intergenic |
| CATTGTGTTGGGTTTGAGAG+AGG | 0.529587 | 4.3:-4757350 | MS.gene55785:CDS |
| GTGCCCATGGATCAACACCC+AGG | 0.532974 | 4.3:+4757996 | None:intergenic |
| AAGTACAAGAAACCAATGTA+AGG | 0.534146 | 4.3:+4757920 | None:intergenic |
| CAAGTTAGTGAGAGTGAGAA+GGG | 0.535305 | 4.3:+4757375 | None:intergenic |
| TGTGGATTGGTTACATGGTG+GGG | 0.539691 | 4.3:-4757404 | MS.gene55785:CDS |
| AGTAGAAACCGAAGAGGGTG+AGG | 0.547358 | 4.3:+4757870 | None:intergenic |
| TGCCCATGGATCAACACCCA+GGG | 0.560771 | 4.3:+4757997 | None:intergenic |
| AGCAACTAGACCTTCATCCC+AGG | 0.562545 | 4.3:+4757271 | None:intergenic |
| ATCGTGTGTGGATTGCCCCT+GGG | 0.578639 | 4.3:-4758013 | MS.gene55785:CDS |
| AATGTGGATTGGTTACATGG+TGG | 0.588190 | 4.3:-4757406 | MS.gene55785:CDS |
| ATGAAAATCACGTTGCAGGA+AGG | 0.588711 | 4.3:+4758090 | None:intergenic |
| GATTTACAGAGAGATACAAC+AGG | 0.591539 | 4.3:+4758139 | None:intergenic |
| ACAAGTTAGTGAGAGTGAGA+AGG | 0.592216 | 4.3:+4757374 | None:intergenic |
| TGAAGGGTTGATAAGTTTAG+GGG | 0.614651 | 4.3:+4758117 | None:intergenic |
| CGAAGGAGGAGAAGAAACCT+GGG | 0.616213 | 4.3:-4757288 | MS.gene55785:CDS |
| GTTGCAGGAAGGTCGTTGAA+GGG | 0.617837 | 4.3:+4758101 | None:intergenic |
| AAGAGAACAGTGATCGTGTG+TGG | 0.618841 | 4.3:-4758025 | MS.gene55785:CDS |
| GAATCAATGGAAGGTGCCCA+TGG | 0.621299 | 4.3:+4757983 | None:intergenic |
| GCCCATGGATCAACACCCAG+GGG | 0.698475 | 4.3:+4757998 | None:intergenic |
| ATGCTCTGTGAATCAATGGA+AGG | 0.701730 | 4.3:+4757974 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATAAATAAAAAGGAAA+AGG | + | chr4.3:4757689-4757708 | None:intergenic | 10.0% |
| !! | TAATTTAATCATTTCAAATG+TGG | + | chr4.3:4757960-4757979 | None:intergenic | 15.0% |
| !!! | CTCTAATTGAACTTTATTTT+CGG | + | chr4.3:4757757-4757776 | None:intergenic | 20.0% |
| ! | AAGAAAAGAAAACTAACTTG+TGG | + | chr4.3:4757619-4757638 | None:intergenic | 25.0% |
| ! | ATTACTTATGTTTAGTTGTG+TGG | - | chr4.3:4757976-4757995 | MS.gene55785:CDS | 25.0% |
| ! | TTGATTTATTATGATTCAGC+TGG | - | chr4.3:4757889-4757908 | MS.gene55785:CDS | 25.0% |
| ! | TTTAATTGTTCTGTTTGGTT+CGG | - | chr4.3:4757912-4757931 | MS.gene55785:CDS | 25.0% |
| !!! | TTTTCTTTGTTTCCTTACAT+TGG | - | chr4.3:4757510-4757529 | MS.gene55785:intron | 25.0% |
| AAGTACAAGAAACCAATGTA+AGG | + | chr4.3:4757525-4757544 | None:intergenic | 30.0% | |
| AATCAGAAAAGCTGGAAATT+CGG | - | chr4.3:4758116-4758135 | MS.gene55785:CDS | 30.0% | |
| CAACGATTAAGTTATTCGAA+CGG | + | chr4.3:4757856-4757875 | None:intergenic | 30.0% | |
| TTAAATGAAAATCACGTTGC+AGG | + | chr4.3:4757359-4757378 | None:intergenic | 30.0% | |
| !! | ACTAACTTGTTCATTGTGTT+GGG | - | chr4.3:4758081-4758100 | MS.gene55785:CDS | 30.0% |
| !! | TTGAAGGGTTGATAAGTTTA+GGG | + | chr4.3:4757329-4757348 | None:intergenic | 30.0% |
| !!! | CAATGTTTTAAAAACCGAAC+TGG | - | chr4.3:4757785-4757804 | MS.gene55785:intron | 30.0% |
| !!! | GGTTCGGTTTTTAGAATATT+GGG | - | chr4.3:4757928-4757947 | MS.gene55785:CDS | 30.0% |
| !!! | TGGTTCGGTTTTTAGAATAT+TGG | - | chr4.3:4757927-4757946 | MS.gene55785:CDS | 30.0% |
| CAAAAAGTAGAAACCGAAGA+GGG | + | chr4.3:4757580-4757599 | None:intergenic | 35.0% | |
| CTTCTTTGTCTAATGTGGAT+TGG | - | chr4.3:4758025-4758044 | MS.gene55785:CDS | 35.0% | |
| GATTTACAGAGAGATACAAC+AGG | + | chr4.3:4757306-4757325 | None:intergenic | 35.0% | |
| TGGAAATTCGGAGAAAAACA+CGG | - | chr4.3:4758128-4758147 | MS.gene55785:CDS | 35.0% | |
| TGGAACTTCTTTGTCTAATG+TGG | - | chr4.3:4758020-4758039 | MS.gene55785:CDS | 35.0% | |
| ! | GCTGGTTTAATTGTTCTGTT+TGG | - | chr4.3:4757907-4757926 | MS.gene55785:CDS | 35.0% |
| !! | CACTAACTTGTTCATTGTGT+TGG | - | chr4.3:4758080-4758099 | MS.gene55785:CDS | 35.0% |
| !! | GTTGAAGGGTTGATAAGTTT+AGG | + | chr4.3:4757330-4757349 | None:intergenic | 35.0% |
| !! | TACTTTTTGCTCGCTTTTGT+TGG | - | chr4.3:4757591-4757610 | MS.gene55785:intron | 35.0% |
| !! | TCTAATGTGGATTGGTTACA+TGG | - | chr4.3:4758033-4758052 | MS.gene55785:CDS | 35.0% |
| !! | TGAAGGGTTGATAAGTTTAG+GGG | + | chr4.3:4757328-4757347 | None:intergenic | 35.0% |
| !!! | GTTAGGAGAAGTTTTGGATT+TGG | + | chr4.3:4757553-4757572 | None:intergenic | 35.0% |
| !!! | TTATTTTGAACAGTTCCAGC+TGG | - | chr4.3:4757705-4757724 | MS.gene55785:intron | 35.0% |
| AACCGAACTGGACATTAAAC+CGG | - | chr4.3:4757797-4757816 | MS.gene55785:intron | 40.0% | |
| ACAAGTTAGTGAGAGTGAGA+AGG | + | chr4.3:4758071-4758090 | None:intergenic | 40.0% | |
| ACAGCATACAGAATGACAGT+TGG | - | chr4.3:4757829-4757848 | MS.gene55785:intron | 40.0% | |
| ACAGTTCCAGCTGGAATTTA+TGG | - | chr4.3:4757714-4757733 | MS.gene55785:intron | 40.0% | |
| ATGAAAATCACGTTGCAGGA+AGG | + | chr4.3:4757355-4757374 | None:intergenic | 40.0% | |
| ATGCTCTGTGAATCAATGGA+AGG | + | chr4.3:4757471-4757490 | None:intergenic | 40.0% | |
| ATTGTGTTGGGTTTGAGAGA+GGG | - | chr4.3:4758093-4758112 | MS.gene55785:CDS | 40.0% | |
| CAAGTTAGTGAGAGTGAGAA+GGG | + | chr4.3:4758070-4758089 | None:intergenic | 40.0% | |
| GCAAAAAGTAGAAACCGAAG+AGG | + | chr4.3:4757581-4757600 | None:intergenic | 40.0% | |
| TTGACAGCTAAGGTGCATTA+TGG | - | chr4.3:4758000-4758019 | MS.gene55785:CDS | 40.0% | |
| !! | AATGTGGATTGGTTACATGG+TGG | - | chr4.3:4758036-4758055 | MS.gene55785:CDS | 40.0% |
| !! | ATGTGGATTGGTTACATGGT+GGG | - | chr4.3:4758037-4758056 | MS.gene55785:CDS | 40.0% |
| AACTGGACATTAAACCGGCA+TGG | - | chr4.3:4757802-4757821 | MS.gene55785:intron | 45.0% | |
| AAGAGAACAGTGATCGTGTG+TGG | - | chr4.3:4757417-4757436 | MS.gene55785:CDS | 45.0% | |
| AGAGAGGGAATCAGAAAAGC+TGG | - | chr4.3:4758108-4758127 | MS.gene55785:CDS | 45.0% | |
| AGCGATGCTCTGTGAATCAA+TGG | + | chr4.3:4757475-4757494 | None:intergenic | 45.0% | |
| CATTGTGTTGGGTTTGAGAG+AGG | - | chr4.3:4758092-4758111 | MS.gene55785:CDS | 45.0% | |
| GAAAAACACGGAATCGAAGG+AGG | - | chr4.3:4758140-4758159 | MS.gene55785:CDS | 45.0% | |
| GACTCACCATAAATTCCAGC+TGG | + | chr4.3:4757723-4757742 | None:intergenic | 45.0% | |
| GGAGAAAAACACGGAATCGA+AGG | - | chr4.3:4758137-4758156 | MS.gene55785:CDS | 45.0% | |
| TGTATGCTGTTGAACCATGC+CGG | + | chr4.3:4757819-4757838 | None:intergenic | 45.0% | |
| ! | GGTGAGGTTAGGAGAAGTTT+TGG | + | chr4.3:4757559-4757578 | None:intergenic | 45.0% |
| !! | TGCCGGTTTAATGTCCAGTT+CGG | + | chr4.3:4757802-4757821 | None:intergenic | 45.0% |
| !! | TGTGGATTGGTTACATGGTG+GGG | - | chr4.3:4758038-4758057 | MS.gene55785:CDS | 45.0% |
| !! | TTCAAAATAAATAAATAAAA+AGG | + | chr4.3:4757695-4757714 | None:intergenic | 5.0% |
| AAACCGAAGAGGGTGAGGTT+AGG | + | chr4.3:4757570-4757589 | None:intergenic | 50.0% | |
| AGTAGAAACCGAAGAGGGTG+AGG | + | chr4.3:4757575-4757594 | None:intergenic | 50.0% | |
| CGAAGGAGGAGAAGAAACCT+GGG | - | chr4.3:4758154-4758173 | MS.gene55785:CDS | 50.0% | |
| GGAGAAGAAACCTGGGATGA+AGG | - | chr4.3:4758161-4758180 | MS.gene55785:CDS | 50.0% | |
| GTTGCAGGAAGGTCGTTGAA+GGG | + | chr4.3:4757344-4757363 | None:intergenic | 50.0% | |
| GTTGTGTGGCTTGACAGCTA+AGG | - | chr4.3:4757990-4758009 | MS.gene55785:CDS | 50.0% | |
| TCGAAGGAGGAGAAGAAACC+TGG | - | chr4.3:4758153-4758172 | MS.gene55785:CDS | 50.0% | |
| TCTCCTAACCTCACCCTCTT+CGG | - | chr4.3:4757564-4757583 | MS.gene55785:intron | 50.0% | |
| !! | GAATCAATGGAAGGTGCCCA+TGG | + | chr4.3:4757462-4757481 | None:intergenic | 50.0% |
| CGTTGCAGGAAGGTCGTTGA+AGG | + | chr4.3:4757345-4757364 | None:intergenic | 55.0% | |
| TGCCCATGGATCAACACCCA+GGG | + | chr4.3:4757448-4757467 | None:intergenic | 55.0% | |
| ! | ATCGTGTGTGGATTGCCCCT+GGG | - | chr4.3:4757429-4757448 | MS.gene55785:CDS | 55.0% |
| GCCCATGGATCAACACCCAG+GGG | + | chr4.3:4757447-4757466 | None:intergenic | 60.0% | |
| GTGCCCATGGATCAACACCC+AGG | + | chr4.3:4757449-4757468 | None:intergenic | 60.0% | |
| ! | GATCGTGTGTGGATTGCCCC+TGG | - | chr4.3:4757428-4757447 | MS.gene55785:CDS | 60.0% |
| !! | GCCCCTGGGTGTTGATCCAT+GGG | - | chr4.3:4757443-4757462 | MS.gene55785:intron | 60.0% |
| !! | TGCCCCTGGGTGTTGATCCA+TGG | - | chr4.3:4757442-4757461 | MS.gene55785:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 4757276 | 4758188 | 4757276 | ID=MS.gene55785 |
| chr4.3 | mRNA | 4757276 | 4758188 | 4757276 | ID=MS.gene55785.t1;Parent=MS.gene55785 |
| chr4.3 | exon | 4757843 | 4758188 | 4757843 | ID=MS.gene55785.t1.exon1;Parent=MS.gene55785.t1 |
| chr4.3 | CDS | 4757843 | 4758188 | 4757843 | ID=cds.MS.gene55785.t1;Parent=MS.gene55785.t1 |
| chr4.3 | exon | 4757729 | 4757746 | 4757729 | ID=MS.gene55785.t1.exon2;Parent=MS.gene55785.t1 |
| chr4.3 | CDS | 4757729 | 4757746 | 4757729 | ID=cds.MS.gene55785.t1;Parent=MS.gene55785.t1 |
| chr4.3 | exon | 4757276 | 4757457 | 4757276 | ID=MS.gene55785.t1.exon3;Parent=MS.gene55785.t1 |
| chr4.3 | CDS | 4757276 | 4757457 | 4757276 | ID=cds.MS.gene55785.t1;Parent=MS.gene55785.t1 |
| Gene Sequence |
| Protein sequence |
