AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55801


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55801.t1 MTR_8g099495 99.048 105 1 0 1 105 175 279 1.99e-73 225
MS.gene55801.t1 MTR_8g099515 43.011 93 48 3 17 104 174 266 2.62e-16 74.7
MS.gene55801.t1 MTR_3g091200 31.897 116 65 4 3 104 145 260 7.97e-16 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55801.t1 AT5G53110 29.412 102 61 2 14 104 157 258 3.74e-11 59.7

Find 42 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGTCCTCAATACTTCATT+TGG 0.314741 4.3:+4552975 None:intergenic
AAGAGACGAATTGGTTGTTT+TGG 0.346013 4.3:-4554289 MS.gene55801:CDS
GAGGAAGATGTGGATTGGTT+GGG 0.360207 4.3:-4554199 MS.gene55801:CDS
ATCTTCCACTTTCACCAAGC+TGG 0.369581 4.3:+4553048 None:intergenic
CTTTCAAGGAAAGTCAAGTA+TGG 0.374180 4.3:-4553263 MS.gene55801:CDS
GTATGGTTTAAGCCTAGGTT+TGG 0.409546 4.3:-4553246 MS.gene55801:CDS
TATGGTTTAAGCCTAGGTTT+GGG 0.424366 4.3:-4553245 MS.gene55801:CDS
GCTTCACAATTTCCACAATC+AGG 0.434686 4.3:+4554227 None:intergenic
TCTTCCACTTTCACCAAGCT+GGG 0.464098 4.3:+4553049 None:intergenic
AGCCTAGGTTTGGGAATACC+TGG 0.477958 4.3:-4553236 MS.gene55801:CDS
GGAGGAAGATGTGGATTGGT+TGG 0.478263 4.3:-4554200 MS.gene55801:CDS
TACCGGTGGAAGAGACGAAT+TGG 0.478685 4.3:-4554298 MS.gene55801:CDS
GTCAAGTATGGTTTAAGCCT+AGG 0.480880 4.3:-4553251 MS.gene55801:CDS
GTCTGCATGTAGGTCTTTCA+AGG 0.481860 4.3:-4553277 MS.gene55801:intron
AGGAAGATGTGGATTGGTTG+GGG 0.491695 4.3:-4554198 MS.gene55801:CDS
AAAACCCAGCTTGGTGAAAG+TGG 0.493961 4.3:-4553053 MS.gene55801:CDS
TTGGGAATACCTGGACTCCT+AGG 0.494340 4.3:-4553227 MS.gene55801:intron
ATTGACTATTTCACCTTGGG+TGG 0.497251 4.3:+4554142 None:intergenic
CAAATGTTGTCACTTGGCCT+AGG 0.507133 4.3:+4553023 None:intergenic
GATGTCCACGGTGTAGCAAA+AGG 0.511204 4.3:+4554350 None:intergenic
GAACATGAATTATTCGATTG+TGG 0.526227 4.3:-4554390 None:intergenic
TATTTCGTCAGCTTTGATAC+CGG 0.536087 4.3:-4554315 MS.gene55801:CDS
AACCAATTCGTCTCTTCCAC+CGG 0.549752 4.3:+4554296 None:intergenic
ACAATTAGACCTAGGAGTCC+AGG 0.561924 4.3:+4553218 None:intergenic
TTCAAACATACCGTTGCAGT+GGG 0.575801 4.3:-4554255 MS.gene55801:CDS
TGTTATGATCTTCCCACCCA+AGG 0.578358 4.3:-4554155 MS.gene55801:intron
ATCAGCCAAATGAAGTATTG+AGG 0.579109 4.3:-4552980 None:intergenic
AGATACCCTAAAACCCAGCT+TGG 0.586664 4.3:-4553062 MS.gene55801:CDS
ATTCAAACATACCGTTGCAG+TGG 0.592135 4.3:-4554256 MS.gene55801:CDS
CAGTGGGATGATCCTGATTG+TGG 0.594321 4.3:-4554239 MS.gene55801:CDS
TTCGTCAGCTTTGATACCGG+TGG 0.597171 4.3:-4554312 MS.gene55801:CDS
TTGACTATTTCACCTTGGGT+GGG 0.599087 4.3:+4554143 None:intergenic
GTGAAAGTGGAAGATTGCCT+AGG 0.611829 4.3:-4553040 MS.gene55801:CDS
ATAGAGCAAATGTTGTCACT+TGG 0.619697 4.3:+4553017 None:intergenic
AATTCACATAATGATGTCCA+CGG 0.636628 4.3:+4554338 None:intergenic
TCAGGATCATCCCACTGCAA+CGG 0.649351 4.3:+4554245 None:intergenic
GAAGCACGTGGAGGAAGATG+TGG 0.650882 4.3:-4554209 MS.gene55801:CDS
ACGTGGAGGAAGATGTGGAT+TGG 0.656368 4.3:-4554204 MS.gene55801:CDS
GTCCACGGTGTAGCAAAAGG+AGG 0.695275 4.3:+4554353 None:intergenic
GTCCAGGTATTCCCAAACCT+AGG 0.704709 4.3:+4553234 None:intergenic
TGTGGAAATTGTGAAGCACG+TGG 0.742242 4.3:-4554221 MS.gene55801:CDS
GGAAATTGTGAAGCACGTGG+AGG 0.769055 4.3:-4554218 MS.gene55801:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATATCAAAATAGATA+CGG - chr4.3:4553380-4553399 MS.gene55801:intron 10.0%
!! AAAAAACATGAAGTTTAATT+TGG - chr4.3:4553487-4553506 MS.gene55801:intron 15.0%
!! AACAAGTTATGTAATTAATT+AGG + chr4.3:4554039-4554058 None:intergenic 15.0%
!!! TTTGATATTTTTTTTTTCCA+AGG + chr4.3:4553373-4553392 None:intergenic 15.0%
!! AAAATCAAATCAAACAAATG+CGG - chr4.3:4553529-4553548 MS.gene55801:intron 20.0%
!! ACATTTGATTCAAATGTATT+TGG + chr4.3:4553672-4553691 None:intergenic 20.0%
!!! TCATGTTTTTTTTTATCTTG+CGG + chr4.3:4553478-4553497 None:intergenic 20.0%
!!! TGCAAATTTAGATCATATTT+TGG - chr4.3:4553273-4553292 MS.gene55801:intron 20.0%
!!! TTTGGTTTTCTTTTAACTTT+TGG - chr4.3:4553291-4553310 MS.gene55801:intron 20.0%
! TGTAATTAATTAGGATCCTT+AGG + chr4.3:4554030-4554049 None:intergenic 25.0%
!!! AATAGTTGTGTTGTTTTCAT+TGG + chr4.3:4553768-4553787 None:intergenic 25.0%
!!! AGTTTCAATTTGGATTAGTT+CGG - chr4.3:4553701-4553720 MS.gene55801:intron 25.0%
!!! ATAGTTGTGTTGTTTTCATT+GGG + chr4.3:4553767-4553786 None:intergenic 25.0%
!!! ATTTTTTTGTGTGTTTAGCT+CGG - chr4.3:4553585-4553604 MS.gene55801:intron 25.0%
!!! TTTGGTTTTGTTTGATTTTG+AGG - chr4.3:4553435-4553454 MS.gene55801:intron 25.0%
!!! TTTTTTTTCCAAGGACTATT+TGG + chr4.3:4553364-4553383 None:intergenic 25.0%
AACTAAACGATTGTGTTTCA+TGG + chr4.3:4553815-4553834 None:intergenic 30.0%
AAGACCCATAACAAAAATTG+GGG + chr4.3:4554268-4554287 None:intergenic 30.0%
AATTCACATAATGATGTCCA+CGG + chr4.3:4553033-4553052 None:intergenic 30.0%
AGAAATATCCAAATAGTCCT+TGG - chr4.3:4553353-4553372 MS.gene55801:intron 30.0%
ATGTAAACACAATTAGACCT+AGG + chr4.3:4554161-4554180 None:intergenic 30.0%
CAAGACCCATAACAAAAATT+GGG + chr4.3:4554269-4554288 None:intergenic 30.0%
TAGGTCTAATTGTGTTTACA+TGG - chr4.3:4554160-4554179 MS.gene55801:CDS 30.0%
TATGCAAAAAGAAGAACACA+AGG - chr4.3:4554187-4554206 MS.gene55801:CDS 30.0%
TCAAGACCCATAACAAAAAT+TGG + chr4.3:4554270-4554289 None:intergenic 30.0%
TGAAACACAATCGTTTAGTT+TGG - chr4.3:4553815-4553834 MS.gene55801:intron 30.0%
TTGAAAATTCAGTGCTTGTT+TGG + chr4.3:4554227-4554246 None:intergenic 30.0%
! ATCATTGACTATTTCACCTT+GGG + chr4.3:4553232-4553251 None:intergenic 30.0%
! GAAACACAATCGTTTAGTTT+GGG - chr4.3:4553816-4553835 MS.gene55801:intron 30.0%
! TTCTTTTCCGTTGTTCTTTT+GGG - chr4.3:4554068-4554087 MS.gene55801:intron 30.0%
! TTTCTTTTCCGTTGTTCTTT+TGG - chr4.3:4554067-4554086 MS.gene55801:intron 30.0%
!! TCGTTTTTGCAGTTTCAATT+TGG - chr4.3:4553691-4553710 MS.gene55801:intron 30.0%
!!! ATTCATAGTGCATTTTGGTT+TGG - chr4.3:4553417-4553436 MS.gene55801:intron 30.0%
!!! GTTTTTCTATTGTGTTGGTT+TGG - chr4.3:4553730-4553749 MS.gene55801:intron 30.0%
!!! TAGTTGTGTTGTTTTCATTG+GGG + chr4.3:4553766-4553785 None:intergenic 30.0%
AATCTCCACTATAAGTCCTA+AGG - chr4.3:4554011-4554030 MS.gene55801:intron 35.0%
AGACCCATAACAAAAATTGG+GGG + chr4.3:4554267-4554286 None:intergenic 35.0%
ATAGAGCAAATGTTGTCACT+TGG + chr4.3:4554354-4554373 None:intergenic 35.0%
CAAATCAAACAAATGCGGTT+TGG - chr4.3:4553534-4553553 MS.gene55801:intron 35.0%
CTTTCAAGGAAAGTCAAGTA+TGG - chr4.3:4554105-4554124 MS.gene55801:intron 35.0%
TATGGTTTAAGCCTAGGTTT+GGG - chr4.3:4554123-4554142 MS.gene55801:intron 35.0%
! AAGAGACGAATTGGTTGTTT+TGG - chr4.3:4553079-4553098 MS.gene55801:intron 35.0%
! CATCATTGACTATTTCACCT+TGG + chr4.3:4553233-4553252 None:intergenic 35.0%
! TATTTCGTCAGCTTTGATAC+CGG - chr4.3:4553053-4553072 MS.gene55801:CDS 35.0%
! TCTTTTCCGTTGTTCTTTTG+GGG - chr4.3:4554069-4554088 MS.gene55801:intron 35.0%
!! GAGGTATTCATAGTGCATTT+TGG - chr4.3:4553412-4553431 MS.gene55801:intron 35.0%
!! TTTTGTTATGGGTCTTGACA+GGG - chr4.3:4554272-4554291 MS.gene55801:CDS 35.0%
!! TTTTTGTTATGGGTCTTGAC+AGG - chr4.3:4554271-4554290 MS.gene55801:CDS 35.0%
!!! AGTTGTGTTGTTTTCATTGG+GGG + chr4.3:4553765-4553784 None:intergenic 35.0%
AGGATCCTTAGGACTTATAG+TGG + chr4.3:4554019-4554038 None:intergenic 40.0%
ATTCAAACATACCGTTGCAG+TGG - chr4.3:4553112-4553131 MS.gene55801:intron 40.0%
ATTGACTATTTCACCTTGGG+TGG + chr4.3:4553229-4553248 None:intergenic 40.0%
GACCCATAACAAAAATTGGG+GGG + chr4.3:4554266-4554285 None:intergenic 40.0%
GCAAATTGAACTGAACCATG+CGG - chr4.3:4553626-4553645 MS.gene55801:intron 40.0%
GCATGGTTCAGTTCAATTTG+CGG + chr4.3:4553627-4553646 None:intergenic 40.0%
GCTTCACAATTTCCACAATC+AGG + chr4.3:4553144-4553163 None:intergenic 40.0%
GTATGGTTTAAGCCTAGGTT+TGG - chr4.3:4554122-4554141 MS.gene55801:intron 40.0%
GTCAAGTATGGTTTAAGCCT+AGG - chr4.3:4554117-4554136 MS.gene55801:intron 40.0%
GTCTACATTTGCAACTACAG+CGG - chr4.3:4553838-4553857 MS.gene55801:intron 40.0%
TTCAAACATACCGTTGCAGT+GGG - chr4.3:4553113-4553132 MS.gene55801:intron 40.0%
TTGACTATTTCACCTTGGGT+GGG + chr4.3:4553228-4553247 None:intergenic 40.0%
! TCGCGGTTTTTCTATTGTGT+TGG - chr4.3:4553725-4553744 MS.gene55801:intron 40.0%
! TTTCACCAAGCTGGGTTTTA+GGG + chr4.3:4554314-4554333 None:intergenic 40.0%
!! ATTTGGATTAGTTCGGTTCG+CGG - chr4.3:4553708-4553727 MS.gene55801:intron 40.0%
!! CAAACAAATGCGGTTTGGTT+TGG - chr4.3:4553539-4553558 MS.gene55801:intron 40.0%
!! CACCCCCCAATTTTTGTTAT+GGG - chr4.3:4554261-4554280 MS.gene55801:CDS 40.0%
!!! CTTTTTTTTACTCAGCCGCA+TGG + chr4.3:4553644-4553663 None:intergenic 40.0%
AACCAATTCGTCTCTTCCAC+CGG + chr4.3:4553075-4553094 None:intergenic 45.0%
ACAATTAGACCTAGGAGTCC+AGG + chr4.3:4554153-4554172 None:intergenic 45.0%
AGATACCCTAAAACCCAGCT+TGG - chr4.3:4554306-4554325 MS.gene55801:CDS 45.0%
ATAGATACGGCGAGAACTAG+AGG - chr4.3:4553393-4553412 MS.gene55801:intron 45.0%
ATCTTCCACTTTCACCAAGC+TGG + chr4.3:4554323-4554342 None:intergenic 45.0%
GTCTGCATGTAGGTCTTTCA+AGG - chr4.3:4554091-4554110 MS.gene55801:intron 45.0%
GTGAAAGTGGAAGATTGCCT+AGG - chr4.3:4554328-4554347 MS.gene55801:CDS 45.0%
TCTTCCACTTTCACCAAGCT+GGG + chr4.3:4554322-4554341 None:intergenic 45.0%
TGCAGACCCCAAAAGAACAA+CGG + chr4.3:4554078-4554097 None:intergenic 45.0%
TGTGGAAATTGTGAAGCACG+TGG - chr4.3:4553147-4553166 MS.gene55801:intron 45.0%
TGTTATGATCTTCCCACCCA+AGG - chr4.3:4553213-4553232 MS.gene55801:intron 45.0%
! AAAACCCAGCTTGGTGAAAG+TGG - chr4.3:4554315-4554334 MS.gene55801:CDS 45.0%
! CAAATGTTGTCACTTGGCCT+AGG + chr4.3:4554348-4554367 None:intergenic 45.0%
! CTTTCACCAAGCTGGGTTTT+AGG + chr4.3:4554315-4554334 None:intergenic 45.0%
! GCACCCCCCAATTTTTGTTA+TGG - chr4.3:4554260-4554279 MS.gene55801:CDS 45.0%
!! AGGAAGATGTGGATTGGTTG+GGG - chr4.3:4553170-4553189 MS.gene55801:intron 45.0%
!! GAGGAAGATGTGGATTGGTT+GGG - chr4.3:4553169-4553188 MS.gene55801:intron 45.0%
!! GCGGTTTGGTTTGGTTGTTA+CGG - chr4.3:4553548-4553567 MS.gene55801:intron 45.0%
!!! TTCTTTTGGGGTCTGCATGT+AGG - chr4.3:4554081-4554100 MS.gene55801:intron 45.0%
ACGTGGAGGAAGATGTGGAT+TGG - chr4.3:4553164-4553183 MS.gene55801:intron 50.0%
AGCCTAGGTTTGGGAATACC+TGG - chr4.3:4554132-4554151 MS.gene55801:intron 50.0%
CAGTGGGATGATCCTGATTG+TGG - chr4.3:4553129-4553148 MS.gene55801:intron 50.0%
GATGTCCACGGTGTAGCAAA+AGG + chr4.3:4553021-4553040 None:intergenic 50.0%
GGAAATTGTGAAGCACGTGG+AGG - chr4.3:4553150-4553169 MS.gene55801:intron 50.0%
GTCCAGGTATTCCCAAACCT+AGG + chr4.3:4554137-4554156 None:intergenic 50.0%
TACCGGTGGAAGAGACGAAT+TGG - chr4.3:4553070-4553089 MS.gene55801:CDS 50.0%
TCAGGATCATCCCACTGCAA+CGG + chr4.3:4553126-4553145 None:intergenic 50.0%
TTGGGAATACCTGGACTCCT+AGG - chr4.3:4554141-4554160 MS.gene55801:intron 50.0%
! ATCCTCCTTTTGCTACACCG+TGG - chr4.3:4553013-4553032 MS.gene55801:CDS 50.0%
! TGGGGGGTGCGTCATAATAA+AGG + chr4.3:4554250-4554269 None:intergenic 50.0%
! TTCGTCAGCTTTGATACCGG+TGG - chr4.3:4553056-4553075 MS.gene55801:CDS 50.0%
!! GGAGGAAGATGTGGATTGGT+TGG - chr4.3:4553168-4553187 MS.gene55801:intron 50.0%
GAAGCACGTGGAGGAAGATG+TGG - chr4.3:4553159-4553178 MS.gene55801:intron 55.0%
GTCCACGGTGTAGCAAAAGG+AGG + chr4.3:4553018-4553037 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 4552982 4554408 4552982 ID=MS.gene55801
chr4.3 mRNA 4552982 4554408 4552982 ID=MS.gene55801.t1;Parent=MS.gene55801
chr4.3 exon 4554156 4554408 4554156 ID=MS.gene55801.t1.exon1;Parent=MS.gene55801.t1
chr4.3 CDS 4554156 4554408 4554156 ID=cds.MS.gene55801.t1;Parent=MS.gene55801.t1
chr4.3 exon 4553228 4553287 4553228 ID=MS.gene55801.t1.exon2;Parent=MS.gene55801.t1
chr4.3 CDS 4553228 4553287 4553228 ID=cds.MS.gene55801.t1;Parent=MS.gene55801.t1
chr4.3 exon 4552982 4553097 4552982 ID=MS.gene55801.t1.exon3;Parent=MS.gene55801.t1
chr4.3 CDS 4552982 4553097 4552982 ID=cds.MS.gene55801.t1;Parent=MS.gene55801.t1
Gene Sequence

>MS.gene55801

ATGAATTATTCGATTGTGGCTATGATGTCTGATCCTCCTTTTGCTACACCGTGGACATCATTATGTGAATTTATTTCGTCAGCTTTGATACCGGTGGAAGAGACGAATTGGTTGTTTTGGACTGATTATTATTCAAACATACCGTTGCAGTGGGATGATCCTGATTGTGGAAATTGTGAAGCACGTGGAGGAAGATGTGGATTGGTTGGGGATGATGCTCTTCGTCTTGCTTGTTATGATCTTCCCACCCAAGGTGAAATAGTCAATGATGTTTTATTTTCACTCATTTCTTGCAAATTTAGATCATATTTTGGTTTTCTTTTAACTTTTGGCGAATTCTTATTTTTAGACTTTTTTTTTTCTTACTTATTAGAAATATCCAAATAGTCCTTGGAAAAAAAAAATATCAAAATAGATACGGCGAGAACTAGAGGTATTCATAGTGCATTTTGGTTTGGTTTTGTTTGATTTTGAGGTTAAAAAGTCATATTAACCGCAAGATAAAAAAAAACATGAAGTTTAATTTGGTCAGTTGACTTTTAAAATGAAAATCAAATCAAACAAATGCGGTTTGGTTTGGTTGTTACGGTTTTTTGCGACACAATTTTTTTGTGTGTTTAGCTCGGTTGCTAAAATAAAAAACCGCAAATTGAACTGAACCATGCGGCTGAGTAAAAAAAAGAAGATCCAAATACATTTGAATCAAATGTCGTTTTTGCAGTTTCAATTTGGATTAGTTCGGTTCGCGGTTTTTCTATTGTGTTGGTTTGGTTTTGAACACCCCCAATGAAAACAACACAACTATTCATAGAAGACTGAAGAAAACACAACCATGAAACACAATCGTTTAGTTTGGGTCTACATTTGCAACTACAGCGGATTTTTTTTTTGAAAATTTTTGTACAAAAAATTATCTTGTTTAAATGTTTATGAGAAAATAAAATAAAAATTATTGATTAAAAGATATTATTTTTTGCTCTAATTAACTCAACTTTTTTTCTTACACAAGAAAATTAACAACTGACTTAGAATCTCCACTATAAGTCCTAAGGATCCTAATTAATTACATAACTTGTTTTTTTCTTTTTCTTTTCCGTTGTTCTTTTGGGGTCTGCATGTAGGTCTTTCAAGGAAAGTCAAGTATGGTTTAAGCCTAGGTTTGGGAATACCTGGACTCCTAGGTCTAATTGTGTTTACATGGATGTTATGCAAAAAGAAGAACACAAGGAATCAAGTACAACGCCAAACAAGCACTGAATTTTCAACCTTTATTATGACGCACCCCCCAATTTTTGTTATGGGTCTTGACAGGGCAACAATCGAAAGATACCCTAAAACCCAGCTTGGTGAAAGTGGAAGATTGCCTAGGCCAAGTGACAACATTTGCTCTATATGTCTTTGTGAGTATCAGCCAAATGAAGTATTGA

Protein sequence

>MS.gene55801.t1

MNYSIVAMMSDPPFATPWTSLCEFISSALIPVEETNWLFWTDYYSNIPLQWDDPDCGNCEARGGRCGLVGDDALRLACYDLPTQGLSRKVKYGLSLGLGIPGLLGQQSKDTLKPSLVKVEDCLGQVTTFALYVFVSISQMKY