Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58196.t1 | XP_003610270.1 | 92.5 | 174 | 12 | 1 | 1 | 173 | 779 | 952 | 2.80E-79 | 304.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58196.t1 | Q9SUG7 | 44.3 | 174 | 91 | 3 | 2 | 173 | 764 | 933 | 3.7e-24 | 112.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58196.t1 | A0A072USH3 | 92.5 | 174 | 12 | 1 | 1 | 173 | 779 | 952 | 2.0e-79 | 304.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene58196 | 0.816819 | 4.54E-52 | -1.69E-46 |
| MS.gene049254 | MS.gene58196 | 0.803689 | 3.09E-49 | -1.69E-46 |
| MS.gene049404 | MS.gene58196 | 0.807176 | 5.74E-50 | -1.69E-46 |
| MS.gene049435 | MS.gene58196 | 0.805584 | 1.24E-49 | -1.69E-46 |
| MS.gene050273 | MS.gene58196 | 0.800711 | 1.27E-48 | -1.69E-46 |
| MS.gene050442 | MS.gene58196 | 0.812357 | 4.42E-51 | -1.69E-46 |
| MS.gene050759 | MS.gene58196 | 0.804337 | 2.27E-49 | -1.69E-46 |
| MS.gene050962 | MS.gene58196 | 0.806391 | 8.42E-50 | -1.69E-46 |
| MS.gene051198 | MS.gene58196 | 0.820161 | 7.94E-53 | -1.69E-46 |
| MS.gene051800 | MS.gene58196 | 0.809005 | 2.34E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene58196 | 0.823099 | 1.66E-53 | -1.69E-46 |
| MS.gene053621 | MS.gene58196 | 0.829106 | 6.20E-55 | -1.69E-46 |
| MS.gene054236 | MS.gene58196 | 0.809791 | 1.59E-50 | -1.69E-46 |
| MS.gene054559 | MS.gene58196 | 0.803714 | 3.06E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene58196 | 0.810954 | 8.91E-51 | -1.69E-46 |
| MS.gene055227 | MS.gene58196 | 0.803459 | 3.45E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene58196 | 0.80247 | 5.53E-49 | -1.69E-46 |
| MS.gene055926 | MS.gene58196 | 0.817471 | 3.24E-52 | -1.69E-46 |
| MS.gene056252 | MS.gene58196 | 0.800077 | 1.71E-48 | -1.69E-46 |
| MS.gene057869 | MS.gene58196 | 0.805514 | 1.29E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene58196 | 0.826571 | 2.52E-54 | -1.69E-46 |
| MS.gene058979 | MS.gene58196 | 0.814466 | 1.52E-51 | -1.69E-46 |
| MS.gene059161 | MS.gene58196 | 0.800902 | 1.16E-48 | -1.69E-46 |
| MS.gene059842 | MS.gene58196 | 0.802874 | 4.56E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene58196 | 0.808546 | 2.94E-50 | -1.69E-46 |
| MS.gene060310 | MS.gene58196 | 0.828274 | 9.85E-55 | -1.69E-46 |
| MS.gene060802 | MS.gene58196 | 0.803973 | 2.70E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58196.t1 | MTR_4g130370 | 92.529 | 174 | 12 | 1 | 1 | 173 | 779 | 952 | 1.48e-104 | 320 |
| MS.gene58196.t1 | MTR_4g130370 | 92.529 | 174 | 12 | 1 | 1 | 173 | 779 | 952 | 1.11e-103 | 320 |
| MS.gene58196.t1 | MTR_4g130370 | 92.529 | 174 | 12 | 1 | 1 | 173 | 779 | 952 | 2.19e-103 | 321 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58196.t1 | AT4G30790 | 53.371 | 178 | 69 | 4 | 2 | 173 | 764 | 933 | 9.60e-46 | 160 |
Find 34 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGATTCATTGCGCAATT+TGG | 0.193397 | 8.4:+4774904 | MS.gene58196:CDS |
| CTCTGTGGTTTGTCCAGACT+TGG | 0.259478 | 8.4:+4774294 | MS.gene58196:CDS |
| GCGCAATTTGGCACAATCTT+TGG | 0.263599 | 8.4:+4774916 | MS.gene58196:CDS |
| CGCAATGAATCTGCAAAATC+AGG | 0.339684 | 8.4:-4774896 | None:intergenic |
| CAACTTGTCATCATTTACTT+TGG | 0.381765 | 8.4:-4774320 | None:intergenic |
| AGATAAGATAGCCGGACAAT+TGG | 0.381849 | 8.4:+4774204 | MS.gene58196:CDS |
| GATACAAAGCTAAAGCTGTT+TGG | 0.385798 | 8.4:+4774394 | MS.gene58196:CDS |
| TTTACTTTGGAACCCAAGTC+TGG | 0.396310 | 8.4:-4774307 | None:intergenic |
| GATAAGATAGCCGGACAATT+GGG | 0.429213 | 8.4:+4774205 | MS.gene58196:CDS |
| AGTAAATGATGACAAGTTGT+TGG | 0.459596 | 8.4:+4774324 | MS.gene58196:CDS |
| AGGAGGTTGCTGTGCTCAAA+GGG | 0.485099 | 8.4:+4774417 | MS.gene58196:CDS |
| GAAGCTACTTGATGAATCTC+AGG | 0.487168 | 8.4:+4774456 | MS.gene58196:CDS |
| ATAGATGAACTGCGCTCACT+TGG | 0.492512 | 8.4:+4774718 | MS.gene58196:intron |
| TGCAAAATCAGGAACACCAC+CGG | 0.497420 | 8.4:-4774885 | None:intergenic |
| AAGACTTGTGTTTATGCTCC+CGG | 0.498645 | 8.4:+4774866 | MS.gene58196:CDS |
| GAGGAGGTTGCTGTGCTCAA+AGG | 0.501720 | 8.4:+4774416 | MS.gene58196:CDS |
| TAGTGACAAACCCAATTGTC+CGG | 0.508868 | 8.4:-4774215 | None:intergenic |
| TTGTTTGCACGAGGCGAGAG+AGG | 0.516785 | 8.4:+4774745 | MS.gene58196:CDS |
| TCTGTGGTTTGTCCAGACTT+GGG | 0.529372 | 8.4:+4774295 | MS.gene58196:CDS |
| TTGCTGTGCTCAAAGGGAGT+TGG | 0.535213 | 8.4:+4774423 | MS.gene58196:CDS |
| AAGCTAAAGCTGTTTGGAGG+AGG | 0.535820 | 8.4:+4774400 | MS.gene58196:CDS |
| CTTGGAGAATTGTTTGCACG+AGG | 0.557223 | 8.4:+4774736 | MS.gene58196:CDS |
| ACAAAGCTAAAGCTGTTTGG+AGG | 0.581879 | 8.4:+4774397 | MS.gene58196:CDS |
| GCAAAATCAGGAACACCACC+GGG | 0.590887 | 8.4:-4774884 | None:intergenic |
| CACAGGCATGCTCTCAGCAG+TGG | 0.597652 | 8.4:-4774248 | None:intergenic |
| TCACATGATCTTGTACCTTG+TGG | 0.601305 | 8.4:+4774271 | MS.gene58196:CDS |
| TCTTGTACCTTGTGGCTCTG+TGG | 0.608549 | 8.4:+4774279 | MS.gene58196:CDS |
| ATACTCTGAGGCCCTCCTGT+CGG | 0.624832 | 8.4:-4774792 | None:intergenic |
| GTGCATCTGTTATTAAGATG+CGG | 0.640517 | 8.4:+4774825 | MS.gene58196:CDS |
| GGTACAAGATCATGTGACAC+AGG | 0.655675 | 8.4:-4774265 | None:intergenic |
| AAAGGGAGTTGGAAGCAAGT+CGG | 0.663136 | 8.4:+4774434 | MS.gene58196:CDS |
| GGACAAACCACAGAGCCACA+AGG | 0.692217 | 8.4:-4774286 | None:intergenic |
| ACGCAATAAACTATACTCTG+AGG | 0.729284 | 8.4:-4774804 | None:intergenic |
| ACTTGTGTTTATGCTCCCGG+TGG | 0.737377 | 8.4:+4774869 | MS.gene58196:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTTGTGTAATAAAAAAGA+TGG | - | chr8.4:4774618-4774637 | None:intergenic | 20.0% |
| !! | ATAAAAAATGTTCTCAGAAA+GGG | + | chr8.4:4774537-4774556 | MS.gene58196:intron | 20.0% |
| !! | TATAAAAAATGTTCTCAGAA+AGG | + | chr8.4:4774536-4774555 | MS.gene58196:intron | 20.0% |
| ! | GATAAAAAAGATAAGATAGC+CGG | + | chr8.4:4774196-4774215 | MS.gene58196:CDS | 25.0% |
| AGTAAATGATGACAAGTTGT+TGG | + | chr8.4:4774324-4774343 | MS.gene58196:CDS | 30.0% | |
| CAACTTGTCATCATTTACTT+TGG | - | chr8.4:4774323-4774342 | None:intergenic | 30.0% | |
| GCAGTTCATCTATATAAAAG+AGG | - | chr8.4:4774711-4774730 | None:intergenic | 30.0% | |
| ACGCAATAAACTATACTCTG+AGG | - | chr8.4:4774807-4774826 | None:intergenic | 35.0% | |
| GATACAAAGCTAAAGCTGTT+TGG | + | chr8.4:4774394-4774413 | MS.gene58196:CDS | 35.0% | |
| GTGCATCTGTTATTAAGATG+CGG | + | chr8.4:4774825-4774844 | MS.gene58196:CDS | 35.0% | |
| AAGACTTGTGTTTATGCTCC+CGG | + | chr8.4:4774866-4774885 | MS.gene58196:CDS | 40.0% | |
| ACAAAGCTAAAGCTGTTTGG+AGG | + | chr8.4:4774397-4774416 | MS.gene58196:CDS | 40.0% | |
| AGATAAGATAGCCGGACAAT+TGG | + | chr8.4:4774204-4774223 | MS.gene58196:CDS | 40.0% | |
| CGCAATGAATCTGCAAAATC+AGG | - | chr8.4:4774899-4774918 | None:intergenic | 40.0% | |
| GAAGCTACTTGATGAATCTC+AGG | + | chr8.4:4774456-4774475 | MS.gene58196:CDS | 40.0% | |
| GATAAGATAGCCGGACAATT+GGG | + | chr8.4:4774205-4774224 | MS.gene58196:CDS | 40.0% | |
| GATGGAAAACAGTGACAGAT+TGG | - | chr8.4:4774600-4774619 | None:intergenic | 40.0% | |
| TAGTGACAAACCCAATTGTC+CGG | - | chr8.4:4774218-4774237 | None:intergenic | 40.0% | |
| TCACATGATCTTGTACCTTG+TGG | + | chr8.4:4774271-4774290 | MS.gene58196:CDS | 40.0% | |
| TGCAGATTCATTGCGCAATT+TGG | + | chr8.4:4774904-4774923 | MS.gene58196:CDS | 40.0% | |
| !! | TTTACTTTGGAACCCAAGTC+TGG | - | chr8.4:4774310-4774329 | None:intergenic | 40.0% |
| AAAAAAGTTCTGCCGACAGG+AGG | + | chr8.4:4774780-4774799 | MS.gene58196:CDS | 45.0% | |
| AAAAAGTTCTGCCGACAGGA+GGG | + | chr8.4:4774781-4774800 | MS.gene58196:CDS | 45.0% | |
| AAAGGGAGTTGGAAGCAAGT+CGG | + | chr8.4:4774434-4774453 | MS.gene58196:CDS | 45.0% | |
| AAGCTAAAGCTGTTTGGAGG+AGG | + | chr8.4:4774400-4774419 | MS.gene58196:CDS | 45.0% | |
| ATAGATGAACTGCGCTCACT+TGG | + | chr8.4:4774718-4774737 | MS.gene58196:intron | 45.0% | |
| CACAAAAAAGTTCTGCCGAC+AGG | + | chr8.4:4774777-4774796 | MS.gene58196:CDS | 45.0% | |
| CTTGGAGAATTGTTTGCACG+AGG | + | chr8.4:4774736-4774755 | MS.gene58196:CDS | 45.0% | |
| GCGCAATTTGGCACAATCTT+TGG | + | chr8.4:4774916-4774935 | MS.gene58196:CDS | 45.0% | |
| GGTACAAGATCATGTGACAC+AGG | - | chr8.4:4774268-4774287 | None:intergenic | 45.0% | |
| TGCAAAATCAGGAACACCAC+CGG | - | chr8.4:4774888-4774907 | None:intergenic | 45.0% | |
| ! | TCTGTGGTTTGTCCAGACTT+GGG | + | chr8.4:4774295-4774314 | MS.gene58196:CDS | 45.0% |
| ACTTGTGTTTATGCTCCCGG+TGG | + | chr8.4:4774869-4774888 | MS.gene58196:CDS | 50.0% | |
| AGGAGGTTGCTGTGCTCAAA+GGG | + | chr8.4:4774417-4774436 | MS.gene58196:CDS | 50.0% | |
| GCAAAATCAGGAACACCACC+GGG | - | chr8.4:4774887-4774906 | None:intergenic | 50.0% | |
| TTGCTGTGCTCAAAGGGAGT+TGG | + | chr8.4:4774423-4774442 | MS.gene58196:CDS | 50.0% | |
| ! | CTCTGTGGTTTGTCCAGACT+TGG | + | chr8.4:4774294-4774313 | MS.gene58196:CDS | 50.0% |
| ! | TCTTGTACCTTGTGGCTCTG+TGG | + | chr8.4:4774279-4774298 | MS.gene58196:CDS | 50.0% |
| GAGGAGGTTGCTGTGCTCAA+AGG | + | chr8.4:4774416-4774435 | MS.gene58196:CDS | 55.0% | |
| GGACAAACCACAGAGCCACA+AGG | - | chr8.4:4774289-4774308 | None:intergenic | 55.0% | |
| TTGTTTGCACGAGGCGAGAG+AGG | + | chr8.4:4774745-4774764 | MS.gene58196:CDS | 55.0% | |
| !! | ATACTCTGAGGCCCTCCTGT+CGG | - | chr8.4:4774795-4774814 | None:intergenic | 55.0% |
| CACAGGCATGCTCTCAGCAG+TGG | - | chr8.4:4774251-4774270 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 4774178 | 4774942 | 4774178 | ID=MS.gene58196 |
| chr8.4 | mRNA | 4774178 | 4774942 | 4774178 | ID=MS.gene58196.t1;Parent=MS.gene58196 |
| chr8.4 | exon | 4774178 | 4774477 | 4774178 | ID=MS.gene58196.t1.exon1;Parent=MS.gene58196.t1 |
| chr8.4 | CDS | 4774178 | 4774477 | 4774178 | ID=cds.MS.gene58196.t1;Parent=MS.gene58196.t1 |
| chr8.4 | exon | 4774722 | 4774942 | 4774722 | ID=MS.gene58196.t1.exon2;Parent=MS.gene58196.t1 |
| chr8.4 | CDS | 4774722 | 4774942 | 4774722 | ID=cds.MS.gene58196.t1;Parent=MS.gene58196.t1 |
| Gene Sequence |
| Protein sequence |
